Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,211,612 A→T 5.2% intergenic (+35/+68) icdC → / ← iraM pseudogene, isocitrate dehydrogenase C‑terminal gene fragment; idcC' is a 54 codon 3' gene fragment created during e14 prophage insertion/RpoS stabilzer during Mg starvation, anti‑RssB factor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,211,6120AT 5.2% 273.5 / 2.5 94intergenic (+35/+68)icdC/iraMpseudogene, isocitrate dehydrogenase C‑terminal gene fragment; idcC' is a 54 codon 3' gene fragment created during e14 prophage insertion/RpoS stabilzer during Mg starvation, anti‑RssB factor
Reads supporting (aligned to +/- strand):  ref base A (50/39);  new base T (2/3);  total (52/42)
Fisher's exact test for biased strand distribution p-value = 6.53e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.71e-01

Only 100 of 101 total aligned reads displayed.

ATGGAAGGCGCAATCAATGCCAAGACCGTAACTTATGACTTCGAACGTCTGATGGAAGGCGCTAAGCTGCTGAAATGTTCAGAGTTTGGTGAAGCGATCATCGAAAACATGTAATCTCTCCATGTGTTAAATATTGAAACGGGCGTATAACACGCCCGTTGTTTTATTTATGTGGATATTATTAATAGCATATCGAGCATATTTATATGAAGCCCATTACTTGAGCCCATATGGGCATATTTTTATAATGCAACTATTATGTAAACATTTATTTGTTATTTTGCTTTCTCCTGGAGG  >  NC_000913/1211464‑1211760
                                                                                                                                                    |                                                                                                                                                    
aTGGAAGGCGCAATCAATGCCAAGACCGTAACTTATGACTTCGAACGTCTGATGGAAGGCGCTAAGCTGCTGAAATGTTCAGAGTTTGGTGAAGCGATCATCGAAAACATGTAATCTCTCCATGTGTTAAATATTGAAACGGGCGtata                                                                                                                                                      >  1:771546/1‑149 (MQ=255)
  ggAAGGCGCAATCAATGCCAAGACCGTAACTTATGACTTCGAACGTCTGATGGAAGGCGCTAAGCTGCTGAAATGTTCAGAGTTTGGTGAAGCGATCATCGAAAACATGTAATCTCTCCATGTGTTAAATATTGAAACGGGCGTATAac                                                                                                                                                    >  1:397402/1‑149 (MQ=255)
   gAAGGCGCAATCAATGCCAAGACCGTAACTTATGACTTCGAACGTCTGATGGAAGGCGCTAAGCTGCTGAAATGTTCAGAGTTTGGTGAAGCGATCATCGAAAACATGTAATCTCTCCATGTGTTAAATATTGAAACGGGCGTATAaca                                                                                                                                                   >  2:505999/1‑149 (MQ=255)
      ggCGCAATCAATGCCAAGACCGTAACTTATGACTTCGAAGCTCTGATGGACGGCGCTAAGCTGCTGAAATGTTCAGAGTTTGGTGAAGCGATCATCGAAAACATGTAATCTCTCCATGTGTTAAATATTGAAACGGGCGTATAACACGc                                                                                                                                                <  2:917001/149‑1 (MQ=255)
      ggCGCAATCAATGCCAAGACCGTAACTTATGACTTCGAACGTCTGATGGAAGGCGCTAAGCTGCTGAAATGTTCAGAGTTTGGTGAAGCGATCATCGAAAACATGTAATCTCTCCATGTGTTAAATATTGAAACGGGCGTGTTATACGc                                                                                                                                                <  1:289474/149‑1 (MQ=255)
         gcAATCAATGCCAAGCCCGTAACTTATGACTTCGAACGTCTGATGGAAGGCGCTAAGCTGCTGAAATGTTCAGAGTTTGGTGAAGCGATCATCGAAAACATGTAATCTCTCCATGTGTTAAATATTGAAACGGGCGTATAACACGCCCg                                                                                                                                             >  1:122085/1‑149 (MQ=255)
         gcAATCAATGCCAAGACCGTAACTTATGACTTCGAACGTCTGATGGAAGGCGCTAAGCTGCTGAAATGTTCAGAGTTTGGTGAAGCGATCATCGAAAACATGTAATCTCTCCATGTGTTAAATATTGAAACGGGCGTATAACACGCCCg                                                                                                                                             >  2:250355/1‑149 (MQ=255)
          caatcaatGCCAAGACCGTAACTTATGACTTCGAACGTCTGATGGAAGGCGCTAAGCTGCTGAAATGTTCAGAGTTTGGTGAAGCGATCATCGAAAACATGTAATCTCTCCATGTGTTAAATATTGAAACGGGCGTATATCACGCCCgt                                                                                                                                            >  1:470792/1‑149 (MQ=255)
          caatcaatGCCAAGACCGTAACTTATGACTTCGAACGTCTGATGGAAGGCGCTAAGCTGCTGAAATGTTCAGAGTTTGGTGAAGCGATCATCGAAAACATGTAATCTCTCCATGTGTTAAATATTGAAACGGGCGTATAACACGCCCgt                                                                                                                                            >  1:340421/1‑149 (MQ=255)
           aatcaatGCCAAGACCGTAACTTATGACTTCTAACGTCTGATGGAAGGCGCGAAGCTGCTGAAATGTTCAGAGTTTGGTGAAGCGATCATCGAAAACATGTAATCTCTCCATGTGTTAAATATTGAAACGGGCGTATAACACGcaagtt                                                                                                                                           >  1:933036/1‑144 (MQ=255)
             tcaatGCCCAGACCGTAACTTTTGACTTCGAACGTCTGATGGAAGGCGCTAAGCTGCTGAAATGTTCAGAGTTTGGTGAAGCGATCATCGAAAACATGTAATCTCTCCATGTGTTAAATATTGAACCGGGCGTGTTATACACCCgtttc                                                                                                                                         <  2:951181/149‑3 (MQ=21)
               aatGCCAAGACCGTAACTTATGACTTCGAACGTCTGATGGAAGGCGCTAAGCTGCTGAAATGTTCAGAGTTTGGTGAAGCGATCATCGAAAACATGTAATCTCTCCATGTGTTAAATATTGAAACGGGCGTATAACACGCCCGTTGttt                                                                                                                                       <  1:715789/149‑1 (MQ=255)
                 tGCCAAGACCGTAACTTATGACTTCGAACGTCTGATGGAAGGCGCTAAGCTGCTGAAATGTTCAGAGTTTGGTGAAGCGATCATCGAAAACATGTAATCTCTCCATGTGTTAAATATTGAAACGGGCGTATAACACGCCCGTTGTttta                                                                                                                                     <  2:606714/149‑1 (MQ=255)
                  gCCAAGACCGTTACTTATGACTTCGAACGTCTGATGGAAGGCGCTAAGCTGCTGAAATGTTCAGAGTTTGGTGAAGCGATCATCGAAAACATGTAATCTCTCCATGTGTTAAATATTGAAACGGGCGTATAACACGCCCGTTGTtttat                                                                                                                                    <  1:803967/149‑1 (MQ=255)
                    cAAGACCGTAACTTATGACTTCGAACGTCTGATGGAAGGCGCTAAGCTGCTGAAATGTTCAGAGTTTGGTGAAGCGATCATCGAAAACATGTAATCTCTCCATGTGTTAAATATTGAAACGGGCGTATAACACGCACGTTGTtttattt                                                                                                                                  >  2:218559/1‑149 (MQ=255)
                      aGACCGGAACATATGACTTCCACCGTCTGATGGAAGGCGCTAAGCTGCGGACATGTTCAGAGTTTGGTGAAGCGATCATCGAAAACATGTACTCTCTCCATGTGTTAAATATTGAAACGGGCGTATAACACGCCCGTTGTTttatttat                                                                                                                                <  2:864830/149‑1 (MQ=255)
                       gACCGTAACTTATGACTTCGAACGTCTGATGGAAGGCGCTAAGCTGCTGAAATGTTCAGAGTTTGGTGAAGCGATCATCGAAAACATGTAATCTCTCCATGTGTTAAATATTGAAACGGGCGTATAACACGCCCGTTGTTttatttatt                                                                                                                               >  1:140876/1‑148 (MQ=255)
                        aCCGTATCTTATGACTTCGAACGTCTGATGGAAGGCGCTAAGCTGCTGAAATGTTCAGAGTTTGGTGAAGCGATCATCGAAAACATGTAATCTCTCCTTGTGTTAAATATTGAAACGGGCGTATAACACGCCCGTTGTTTTATTTAtgt                                                                                                                              <  1:237793/149‑1 (MQ=255)
                         ccGTCACTTCTGCCTGGGAACGTCTGCTGGAAGGCGCTAAGCTGCTGAAAGGGTCCGAGTTTGGTGAAGCGATCATCGAAAACAAGTAATCTCTCCATGTGTTAAATATTGAAACGGGCGGATAACACGCCCGTTGTTTTATTTAtgtg                                                                                                                             <  2:61662/149‑1 (MQ=255)
                          cttAACTTCTGACTTCGAACGTCTGATGGAAGGCGCTATGCTGCTGAAATGTTCAGAGTTTGGTGAAGCGATCATCGAAAACAGGTAATCTCTCCATGTGTAAAATATTGAAACGGGCGTATAACACGCCCGTTGTTTTATTTATGTgg                                                                                                                            <  1:758325/147‑1 (MQ=255)
                          cGTAACTTATGACTTCGAACGTCTGATGGAAGGCGCTACGCTGCTGAAAAGTTCAGAGTTTGGTGAAGCGATCATCGAAAACATGTAATCTCTCCATGTGTTAAATATTGAAACGGGCGTATAACACGCACGTTGTATTATTTATTTgg                                                                                                                            >  1:1000215/1‑149 (MQ=25)
                            tAACTTATGACTTCGAACGTCTGATGGAAGGCGCTAAGCTGCTGAAATGTTCAGAGTTTGGTGAAGCGATCATCGAAAACATGTAATCTCTCCATGTGTTAAATATTGAAACGGGCGTATAACACGCCCGTTGTTTTATTTATGTGGat                                                                                                                          >  1:443180/1‑149 (MQ=255)
                            tAACTTATGACTTCGAACGTCTGATGGAAGGCGCTAAGCTGCTGAAATGTTCAGAGTTTGGTGAAGCGATCATCGAAAACATGTAATCTCTCCATGTGTTAAATATTGAAACGGGCGTATAACACGCCCGTTGTTTTATTTATGTGGat                                                                                                                          >  2:53859/1‑149 (MQ=255)
                             aaCTTATGACTTCGAACGTCTGATGGAAGGCGCTAAGCTGCTGAAATGTTCAGAGTTTGGTGAAGCGATCATCGAAAACATGTAATCTCTCCATGTGTTAAATATTGAAACGGGCGTATAACACGCCCGTTGTTTTATTTATGTGGata                                                                                                                         >  1:10786/1‑149 (MQ=255)
                              aCTTATGTCTTCGAACGTCTGATGGAAGGCGCTAAGCTGCTGAAATGTGCAGACTTTGGTGAAGCGATCATCGAAAACATGTAATCTCTCCATGTGTTAAATATTGAAACGGGCGTATAACACGCCCGTTGTTTTATTTATGTGGatat                                                                                                                        <  1:428903/149‑1 (MQ=255)
                                 tatttcatCGAACGTCTGTTGGAAGGCGCTAAGCTGGTGAAATGTTCAGATTTTGGTGTAGCGATCATCGAAAACATGTAATCTCTCCATGTGTTAAATATTGAAACGGGCGTATAACACGCCCGTTGTTTTATTTATGTGGAtattat                                                                                                                     <  2:949929/142‑1 (MQ=37)
                                 tATGACTTCGAACGACTGATGGAAGTCGCTAAGCTGCTTATATGTTCAGTGTTTGGTGAAGCTATCATCGAAAACATGTAATCTCTCCATGTGTTAAATATTGAAACGGGCGTATAACACGCCCGTTGTTTTATTTATGTGGAtattat                                                                                                                     <  2:50889/149‑1 (MQ=255)
                                    gACTTCGAACGTCTGATGGAAGGCGCTAAGCTGCTGAAATGTTCAGAGTTTGGTGAAGCGATCATCGAAAACATGTAATCTCTCCATGTGTTAAATATTTAAACGGGCGTATAACACGCCCGTTGTTTTATTTATGTGGATATTATTaa                                                                                                                  <  1:718381/149‑1 (MQ=255)
                                     aCTTCGAACGTCTGATGGAAGGCGCTAAGCTGCTGAAATGTTCAGAGTTTGGTGAAGCGATCATCGAAAACATGTAATCTCTCCATGTGTTAAATATTGAAACGGGCGTATAACACGCCCGTGGTTTTATTTATGTGGATATTATTAAt                                                                                                                 >  2:1003438/1‑149 (MQ=255)
                                       ttCGAACGTCTGATGGAAGGCGCTAAGCTGCTGAACTGTTCAGAGTTTGGTGGAGCGATCATCGAAAACATGTAAGCTCTCCATTGGTTAAATATTGAAACGGGCCTATAACACGCCCGTTGTTTTATTTATGTGGATATTATTAATat                                                                                                               >  2:563344/1‑148 (MQ=37)
                                             cGTCTGATGGAAGGCGCTAAGCTGCTGAAATGTTCAGAGTTTGGTGAAGCGATCATCGAAAACATGTAATCTCTCCATGTGTTAAATATTGAAACGGGCGTATAACACGCCCGTTGTTTTATTTATGTGGATATTATTAATAGCATATc                                                                                                         <  2:321779/149‑1 (MQ=255)
                                                cTGATGGAAGGCGCTAAGCTGCTGAAATGTTCAGAGTTTGGTGAAGCGATCATCGAAAACATGTAATCTCTCCATGTGTTAAATATTGAAACGGGCGTATAACACGCCCGTTGTTTTATTTATGTGGATATTATTAATAGCATATCGAg                                                                                                      <  2:797993/149‑1 (MQ=255)
                                                 tGATGGAAGGCGCTAATCTGCTGAAATGTTCAGAGTTTGGTGAAGCGATCATCGAAAACATGTAATCTCTCCATGTGTTAAATATTGAAACGGGCGTATAACACGCCCGTTGTTTTATTTATGTGGATATTATTAATAGCATATCGAGc                                                                                                     <  1:1015870/149‑1 (MQ=255)
                                                 tGATGGAAGGCGCTAAGCTGCTGAAATGTTCAGAGTTTGGTGAAGCGATCATCGAAAACATGTAATCTCTCCATGTGTTAAATATTGAAACGGGCGTATAACACGCCCGTTGTTTTATTTATGTGGATATTATTAATAGCATATCGAGc                                                                                                     >  1:512941/1‑149 (MQ=255)
                                                 tGATGGAAGGCGCTAAGCTGCTGAAATGTTCAGAGTTTGGTGAAGCGATCATCGAAAACATGTAATCTCTCCATGTGTTAAATATTGAAACGGGCGTATAACAAGCCCGTTGTGTTATTTATGTGGATATTATTAATAGAATATCGAGc                                                                                                     >  2:710154/1‑149 (MQ=255)
                                                   aTGGAAGGAGCTAAGCTGCTGAAATGTTCAGAGTTTGGTGAAGCGATCATCGAAAACATGTAATCTCTCCATGTGTTAAATATTGAAACGGGCGTATAACACGCCCGTTGTTTTATTTATGTGGATATTATTAATAGCATATCGAGCat                                                                                                   <  2:1030216/149‑1 (MQ=255)
                                                   aTGGAAGGAGCTAAGCTGATGAAATGTTCAGAGTTTGGTGAAGCGATCATCGAAAACATGTAATCTCTCCATGTGTTAAATATTGAAACGGGCGTATAACACGCCCGTTGTTTTATTTATGTGGATATTATTAATAGCATATCGAGCat                                                                                                   <  2:1027055/149‑1 (MQ=255)
                                                    tGGAAGGCGCTAAGCTGCTGAAATGTTCAGAGTTTGGTGAAGCGATCATCGAAAACATGTAATCTCTCCATGTGTTAAATATTGAAACGGGCGTATAACACGCCCGTTGTTTTATTTATGTGGATATTATTAATAGCATATCGAGCata                                                                                                  <  1:108661/149‑1 (MQ=255)
                                                        aGGCGCTAAGCTGCTGAAATGTTCAGAGTTTGGTGAAGCGATCATCGAAAACATGTAATCTCTCCATGTGTTAAATATTGAAACGGGCGTATAACACGCCCGTTGTTTTATTTATGTGGATATTATTAATAGCATATCGAGCATATTta                                                                                              >  1:820206/1‑149 (MQ=255)
                                                            gcTAAGCTGCTGAAATGTTCAGAGTTTGGTGAAGCGATCATCGAAAACATGTAATCTCTCCATGTGTTAAATATTGAAACGGGCGTATAACACGCCCGTTGTTTTATTTATGTGGATATTATTAATAGCATATCGAGCATATTTATATg                                                                                          >  1:113461/1‑149 (MQ=255)
                                                              tAAGCTGCTGAAATGTTCAGAGTTTGGTGAAGCGATCATCGAAAACATGTAATCTCTCCATGTGTTAAATATTGAAACGGGCGTATAACACGCCCGTTGTTTTATTTATGTGGATATTATTAATAGCATATCGAGCATATTTATATGaa                                                                                        >  1:939345/1‑149 (MQ=255)
                                                                  ctgctgAAATGTTCAGAGTTTGGTGAAGCGATCATCGAAAACATGTAATCTCTCCATGTGTTAAATATTGAAACGGGCGTATAACACGCCCGTTGTTTTATTTATGTGGATATTATTAATAGCATATCGAGCATATTTATATGAATccc                                                                                    >  2:547970/1‑149 (MQ=255)
                                                                  ctgctgAAATGTTCAGAGTTTGGTGAAGCGATCATCGAAAACATGTAATCTCTCCATGTGTTAAATATTGAAACGGGCGTATAACACGCCCGTTGTTTTATTTATGTGGATATTATTAATAGCATATCGAGCATATTTATATGAAGccc                                                                                    <  2:132957/149‑1 (MQ=255)
                                                                    gctgAAATGTTCAGAGTTTGGTGAAGCGATCATCGAAAACATGTAATCTCTCCATGTGTTAAATATTGAAACGGGCGTATAACACGCCCGTTGTTTTATTTTTGTGGATATTATTAATAGCATATCGAGCATATTTATATGAAGCCCAt                                                                                  >  1:636191/1‑149 (MQ=255)
                                                                      tgAAATGTTCAGAGTTTGGTGAAGCGATCATCGAAAACATGTAATCTCTCCATGTGTTAAATATTGAAACGGGCGTATAACACGCCCGTTGTTTTATTTATGTGGATATTATTAATAGCATATCGAGCATATTTATATGAAGCCCATTa                                                                                >  1:454408/1‑149 (MQ=255)
                                                                       gAAATGTTCAGAGTTTGGAGAAGCGATCATCGAAAACATGTAATCTCTCCATGTGTTAAATATTGAAACGGGCGTATAACACGCCCGTTGTTTTATTTATGTGGATATTATTAATAGCATATCGAGCATATTTATATGAAGCCCATTAc                                                                               <  1:868196/149‑1 (MQ=255)
                                                                        aaaTGTTCAGAGTTTGGTGAAGCGATCATCGAAAACATGTAATCTCTCCATGTGTTAAATATTGAAACGGGCGTATAACACGCCCGTTGTTTTATTTATGTGGATATTATTAATAGCATATCGAGCATATTTATATGAAGCCCATTTCt                                                                              >  1:647174/1‑149 (MQ=255)
                                                                         aaTGTTCAGAGTTTGGTGAAGCGATCATCGAAAACATGTAATCTCTCCATGTGTTAAATATTGAAACGGGCGTATAACACGCCCGTTGTTTTATTTATGTGGATATTATTAATAGCATATCGAGCATATTTATATGAAGCCCATTACtt                                                                             <  1:250355/149‑1 (MQ=255)
                                                                           tGTTCAGAGTTTGGTGAAGCGATCATCGAAAACATGTAATCTCTCCATGTGTTAAATATTGAAACGGGCGTATAACACGCCCGTTGTTTTATTTATGTGGATATTATTAATAGCATATCGAGCATATTTATATGAAGCCCATTACTTGa                                                                           <  2:715698/149‑1 (MQ=255)
                                                                              tCAGAGTTTGGTGAAGCGATCATCGAAAACATGTAATCTCTCCATGTGTTAAATATTGAAACGGGTGTATAACACGCCCGTTGTTTTATTTATGTGGATATTATTAATAGCATATCGAGCATATTTATATGAAGCCCATTACTTGAGcc                                                                        >  1:255929/1‑149 (MQ=255)
                                                                               cAGAGTTTGGTGAAGCGATCATCGAAAACATGTAATCTCTCCATGTGTTAAATATTGAAACGGGCGTATAACACGCCCGTTGTTTTATTTATGTGGATATTATTAATAGCATATCGAGCATATTTATATGAAGCCAATTACTTGAGccc                                                                       >  1:732480/1‑149 (MQ=255)
                                                                                agagTTTGGTGAAGCGATCATCGAAAACATGTATTCTCTCCATGTGTTTAATATTGAAACGGGCGGATAACACGCCCGTTTTGTTATTTATGTGGATATTTATAATAGCATAGCGTGATTAGTTATTTGAACCCGATTACGTACGCCCa                                                                      >  2:661580/1‑149 (MQ=255)
                                                                                     ttGGTGAAGCGATCATCGAAAACATGTAATCTCTCCATGTGTTAAATATTGAAACGGGCGTATAACCCGCCCGTTGTTTTATTTATGTGGATATTATTAATAGCATATCGAGCATATTTATATGAAGCCCATTACTTGAGCCCATATgg                                                                 <  2:696950/149‑1 (MQ=255)
                                                                                         tGAAGCGATCATCGATAACATGTAATCTCTCCATGTGTTAAATATTGAAACGGGCGTATAACACGCACGTTGTTTTATTTATGTGGATATTATTAATAGAATATAGAGCATATTTATATGAAGCCCATTACTTGAGCACATATgtgaat                                                             >  1:600614/1‑144 (MQ=255)
                                                                                         tGAAGCGATCATCGAAAACATGTAATCTCTCCATGTGTTAAATATTGAAACGGGCGTATAACACGCCCGTTGTTTTATTTATGTGGATATTATTAATAGCATATCGAGCATATTTATATGAAGCCCATTACTTGAGCCCATATGGGCat                                                             >  1:823989/1‑149 (MQ=255)
                                                                                         tGAAGCGATCATCGAAAACAAGTAATCTCTCCATGTGTTAAATATTGAAACGGGCGTATAACACGCCCGTTGTTTTATTTATGTGGATATTATTAATAGCATATCGAGCATATTTATATGAAGCCCATTACTTGAGCCCATATGGGCat                                                             >  2:1321/1‑149 (MQ=255)
                                                                                          gAAGCGATCATCGAAAACATGTAATCTCTCCATGTGTTAAATATTGAAACGGGCGTTTATCACGCCCGTTGTTTTATTTATGTGGATATTATTAATAGCATATCGAGCATATTTATATGAAGCCCATTACTTGAGCCCATATGGGCata                                                            >  2:667950/1‑149 (MQ=255)
                                                                                              cGATCATCGAAAACATGTAATCTCTCCATGTGTTAAATATTGAAACGGGCGTATAACACGCCCGTTGTTTTATTTATGTGGATATTATTAATAGCATATCGAGCATATTTATATGAAGCCCATTACTTGAGCCCATATGGGCATAtttt                                                        <  2:122085/149‑1 (MQ=255)
                                                                                               gATCATCGAAAACATGTAATCTCTCCATGTGTTAAATATTGAAACGGGCGTATAACACGCCCGTTGTTTTATTTATGTGGATATTATTAATAGCATATCGAGCATATTTATATGAAGCCCATTACTTGAGCCCATATGGGCATAttttt                                                       >  2:573182/1‑149 (MQ=255)
                                                                                                atcatcGAAAACATGTAATCTCTCCATGTGTTAAATATTGAAACGGGCGTATAACACGCCCGTTGTTTTATTTATGTGGATATTATTAATAGCATATCGAGCATATTTATATGAAGCCCATTACTTGAGCCCATATGGGCATATTTTta                                                      <  1:842943/149‑1 (MQ=255)
                                                                                                    tcGAAAACATGTAATCTCTCCATGTGTTAAATATTGAAACGGGCGTATAACACTCCCGTTGTTGTATTTATGTGGATATTATTAATAGCATATCGAGCATATTTATATGAAGCCCATTACTTGAGCCCATATGGGCATATTTTTATAAt                                                  >  2:776019/1‑149 (MQ=255)
                                                                                                     cGAAAACATGTAATCTCTCCATGTGTTAAATATTGAAACGGGCGTATAACACGCCCGTTGTTTTATTTATGTGGATATTATTAATAGCATATCGAGCATATTTATATGAAGCCCATTACTTGAGCCCATATGGGCATATTTTTATAATg                                                 <  1:985763/149‑1 (MQ=255)
                                                                                                     agAAAACATGTAATCTCTCCATGTGTTAAATATTGAAACGGGCGTATAACACGCCCGTTGTTTTATTTATGTGGATTTTATTAATAGCATATCGAGCATATTTATATGAAGCCCATTACTTGAGCCCATATGGGCATATTTTTATAATg                                                 <  2:537562/148‑1 (MQ=255)
                                                                                                      gAAAACATGTAATCTCTCCATGTGTTAAATATTGAAACGGGCGTATAACACGCCCGTTGTTTTATTTATGTGTATATTATTAATAGCATATCGAGCATATTTATATGAAGCCCATTACTTGATCCAATATTGGCATAGTTTTATAATga                                                >  1:673457/1‑148 (MQ=255)
                                                                                                      gAAAACATGTAATCTCTCCATGTGTTAAATATTGAAACGGGCGTATAACACGCCCGTTGTTTTATTTATGTGGATATTATTAATAGCATATCGAGCATATTTATATGAAGCCCATTACTTGAGCCCATATGGGCATATTTTTATAATGc                                                <  2:959634/149‑1 (MQ=255)
                                                                                                          aCATGTAATCTCTCCATGTGTTAAATATTGAAACGGGCGTATAACACGCCCGTTGTTTTATTTATGTGGATATTATTAATAGCATATCGAGCATATTTATATGAAGCCCATTACTTGAGCCCATATGGGCATATTTTTATAATGCAACt                                            <  2:842389/149‑1 (MQ=255)
                                                                                                           cATGTAATCTCTACATGTGTTAAATATTGAAACGGGCGTATAACACGCCGGTTGTTTTATTTATGTGGATATTATTAATAGCATATCGAGCATATTTATATGAAGCCCATTACTTGAGCCCATATGGGCATATTTTTATAATGCAACta                                           <  2:852133/149‑1 (MQ=255)
                                                                                                             tGTAATCTCTCCATGTGTTAAATATTGAAACGGGCGTATAACACGCCCGTTGTTTTATTTATGTGGATATTATTAATAGCATATCGAGCATATTTATATGAAGCCCATTACTTGAGCCCATATGGGCATATTTTTATAATGCAACtatt                                         <  2:340421/149‑1 (MQ=255)
                                                                                                             tGTAATCTCTCCATGTGGTAAATATTGAAACGGGCGTATAACACGCCCGTTGTTTTAATTATGTGGATATTATTAATAGCATAGCGAGCATATTTATATGAAGCCCATTACTTGAGCCCATAAGGGCATATTTTTATAATGCAACtatt                                         <  1:774816/149‑1 (MQ=255)
                                                                                                              gTAATCTCTCCATGTGTTAAATATTGAAACGGGCGTATAACACGCCCGTTGTTTTATTTATGTGGATATTATTTATAGCATATCGAGCATATTTATATGAAGCCCATTACTTGAGCCCATATGGGCATATTTTTATAATGCAACtatta                                        >  2:1012918/1‑149 (MQ=255)
                                                                                                              gTAATCTCTCCATGTGTTAAATATTGAAACGGGCGTATAACACGCCCGTTGTTTTATTTATGTGGATATTATTAATAGCATATCGAGCATATTTATATGAAGCCCATTACTTGAGCCCATATGGGCATATTTTTATAATGCAACtatta                                        >  2:573972/1‑149 (MQ=255)
                                                                                                               tAATCTCTCCATGTGTTAAATATTGAAACGGGCGTATAACACGCCAGTTGTTTTATTTATGTGGATATTATTAATAGCATATATAGCATATTTATATGAATCCCATTACTTGAGCCCATATTGTAATATTTTTATAATGAAACtattat                                       >  2:194525/1‑149 (MQ=255)
                                                                                                                aaTCTCTCCATGTGTTAAATATTGAAACGGGCGTATAACACGCCCGTTGTTTTATTTATGTGGATATTATTAATAGCATATCGAGCATATTTATATGAAGCCCATTACTTGAGCCCATATGGGCATATTTTTATAATGCAACtattatt                                      >  1:115261/1‑148 (MQ=255)
                                                                                                                      tcCATGTGTTAAATATTGAAACGGGCGTATAACACGCCCGTTGTTTTATTTATGTGGATATTATTAATAGCATATCGAGCATATTTATATGAAGCCCATTACTTGAGCCCATATGGGCATATTTTTATAATGCAACTATTATGTAAACa                                >  2:346868/1‑149 (MQ=255)
                                                                                                                        cATGTGTTAAATATTGAAACGGGCGTATAACACGCCCGTTGTTTTATTTATGTGGATATTATTAATAGCATATCGAGCATATTTATATGAAGCCCATTACTTGAGCCCATATGGGCATATTTTTATAATGCAACTATTATGTAAACatt                              >  1:108122/1‑149 (MQ=255)
                                                                                                                           gttttAAATATTGAAACGGGCGGATAACACGCACGTTGTTTTATTTATGTGTATATTATTTATAGCATATCTAGCATATTTATATGAAGCCCATTACTTGAGCCCATATGGGCATATTTTTATAATGCAACTATTATGTAAACatttat                           <  1:466063/146‑1 (MQ=255)
                                                                                                                            tgtTAAATATTGAAACGGGCGTATTACACGCCCGTTGTTTTATTTATGTGGATATTATTAATAGAATATCGAGCATATTTATATGAAGCCCATAACTTGAGCCCATATGGGCATTTTTTTATAATGCAACTATTATGTAAACatttatt                          >  1:320976/1‑149 (MQ=255)
                                                                                                                             gtTAAATATTGAAACGGGCGTATAACACGCCCGTTGTTTTATTTATGTGGATATTATTAATAGCATATCGAGCATATTTATATGAAGCCCATTACTTGAGCCCATATGGGCATATTTTTATAATGCAACTATTATGTAAACatttattt                         <  2:514500/149‑1 (MQ=255)
                                                                                                                             gtTAAATATTGAAACGGGCGTATAACACGCCCGTTGTTTAATTTATGTGGATATTATTAATAGCATATCGAGCATATTTATATGAAGCCCATTACTTGAGCCCATATGGGCATATTTTTATAATGCAACTATTATGTAAACatttattt                         <  2:470792/149‑1 (MQ=255)
                                                                                                                              ttAAATATTGAAACGGGCGTATAACACGCCCGTTGTTTTATTTATGTGGATATTATTAATAGCATATCGAGCATATTTATATGAAGCCCATTACTTGAGCCCATATGGGCATATTTTTATAATGCAACTATTATGTAAACATTTATTTg                        >  1:270644/1‑149 (MQ=255)
                                                                                                                              ttAAATATTGAAACGGGCGTATAACACGCCCGTTGTTTTATTTATGTGGATATTATTAATAGCATATCGAGCATATTTATATGAAGCCCATTACTTGAGCCCATATGGGCATATTTTTATAATGCAACTATTATGTAAACATTTATTTg                        >  2:379843/1‑149 (MQ=255)
                                                                                                                              ttAAATATTGAAACGGGCGTATAACACGCCCGTGGTTTTATTTATGTGGATATTATTAATAGCATATCGAGCATATTTATATGAAGCCCATTACTCGAGCCCATATGGGCATATTTTTATAATGCAACTATTATGTAAACATTTATTTg                        >  2:821458/1‑149 (MQ=255)
                                                                                                                               tAAATATTGAAACGGGCGTATAACACGCCCGTTGTTTTATTTATGTGGATATTATTAATAGCATATCGAGCATATTTATATGAAGCCCATTACTTGAGCCCATATGGGCATATTTTTATAATGCAACTATTATGTAAACATTTATTTGt                       >  2:536472/1‑149 (MQ=255)
                                                                                                                                aaaTATTGAAACGGGCGTATAACACGCCCGTTGTTTTATTTATGTGGATATTATTAATAGCATATCGAGCATATTTATATGAAGCCCATTACTTGAGCCCATATGGGCATATTTTTATAATGCAACTATTATGTAAACTTTTATTTGtt                      >  1:192416/1‑149 (MQ=255)
                                                                                                                                aaaTATTGAAACGGGCGTATAACACGCCCGTTGTTTTATTTATGTGGATATTATTAATAGCATATCGAGCATATTTATATGAAGCCCATTACTTGAGCCCATATGGGCATATTTTTATAATGCAACTATTATGTAAACATTTATTTGtt                      <  2:10786/149‑1 (MQ=255)
                                                                                                                                 aaTATTGAAACGGGCGTATAACACGCCCGTTGTTTTATTTATGTGGATATTATTAATAGCATATCGAGCATATTTATATGAAGCCCATTACTTGAGCCCATATGGGCATATTTTTATAATGCAACTATTATGTAAACATTTATTTGTTa                     >  1:425362/1‑149 (MQ=255)
                                                                                                                                    atTGAAACGGGCGTATAACACGCCCGTTGTTTTATTTATGTGGATATTATTAATAGCATATCGAGCATATTTATATGAAGCCCATTACTTGAGCCCATATGGGCATATTTTTATAATGCAACTATTATGTAAACATTTATTTGTTAttt                  <  2:113461/149‑1 (MQ=255)
                                                                                                                                    atTGAAACGGGCGTATAACACGCCCGTTGTTTTATTATGTGGATATTATTAATAGCATATCGAGCATATTTATATGAAGCCCATTACTTGAGCCCATATGGGCATATTTTTATAATGCAACTATTATGTAAACATTTATTTGTTAtttt                 <  2:473647/149‑1 (MQ=255)
                                                                                                                                     ttGAAACGGGCGTATAACACGCCCGTTGTTTTATTTATGTGGATATTATTAATAGCATATCGAGCATATTTATATGAAGCCCATTACTTGAGCCCATATGGGCATATTTTTATAATGCAACTATTATGTAAACATTTATTTGTTAtttt                 >  2:437637/1‑149 (MQ=255)
                                                                                                                                       gAAACGGGCGTATAACACGCCCGTTGTTTTATTTATGTGGATATTATTAATAGCATATCGAGCATATTTATATGAAGCCCATTACTTGAGCCCATATGGGCATATTTTTATAATGCAACTATTATGTAAACATTTATTTGTTATTTTGc               >  2:714275/1‑149 (MQ=255)
                                                                                                                                       gAAACGGGCGTATAACACGCCCGTTGTTTTATTTATGTGGATATTATTAATAGCATATCGAGCATATTTATATGAAGCCCATTACTTGAGCCCATATGGGCATATTTTTATAATGCAACTATTATGTAAACATTTATTTGTTATTTTGc               <  1:53859/149‑1 (MQ=255)
                                                                                                                                         aaCGGGCGTATTACAGGCGCGTTGTTTTATTTATGTGGATATTATTTATAGGATATCGAGCATATTTTTATGAAGCCCATTACTTGAGCCAATATGGGCATATTTTTATAATGCTTCTATTATGTTAACATTTATTTGTTATTTTGCtt             <  2:257541/149‑1 (MQ=255)
                                                                                                                                           cGGGCGTGTTATACGTCCGTTGTTTTATTTATGTGGATATTATTAATAGCATATCGAGCATATTTATATGAAGCCAATTACTTGAGCCCATATGGGCATATTTTTATAATGCAACTATTATGTAAACATTTATTTGTTATTTTGCttta           >  1:367566/1‑148 (MQ=255)
                                                                                                                                               cGTATAACACGCCCGTTGTTTTATTTATGTGGATATTATTAATAGCATATCGAGCATATTTATATTAAGCCCATTACTTGAGCCCATATGGGCATATTTTTATAATGCAACTATTATGTAAACATTTATTTGTTATTTTGCTTTCTCCt       >  1:589131/1‑149 (MQ=255)
                                                                                                                                                gTATAACACGCCCGTTGTTTTATTTATGTGGATATTATTAATAGCATATCGAGCATATTTATATGAAGCCCATTACTTTAGCCAATATGGGCATATTTTTATAATGCAACTATTATGTAAACATTTATTTGTTATTTTGCTTTCTCCTg      >  1:379636/1‑149 (MQ=255)
                                                                                                                                                 tataACACGCCCGTTGTTTTATTTATGTGGATATTATTAATAGCATATCGAGCATATTTATATGAAGCCCATTACTTGAGCCCATATGGGCATATTTTTATAATGCAACTATTATGTAAACATTTATTTGTTATTTTGCTTTCTCCTgg     <  1:573182/149‑1 (MQ=255)
                                                                                                                                                  ataACACGCCCGTTGTTTTATTTATGTGGATATTATTAATAGCATATCGAGCATATTTATATGAAGCCCATTACTTGAGCCCATATGGGCATATTTTTATAATGCAACTATTATGTAAACATTTATTTGTTATTTTGCTTTCTCCTggg    >  1:726415/1‑148 (MQ=255)
                                                                                                                                                  ataACACGCCCGTTGTTTTATTTATGTGGATATTATTAATAGCATATCGAGCATATTTATATGAAGCCCATTACTTGAGCCCATATGGGCATATTTTTATAATGCAACTATTATGTAAACATTTATTTGTTATTTTGCTTTCTCCTgga    <  1:505999/149‑1 (MQ=255)
                                                                                                                                                   taACACGCCCGTTGTTTTATTTATGTGGATATTATTAATAGCATATCGAGCATATTTATATGAAGCCCATTACTTGAGCCCATATGGGCATATTTTTATAATGCAACTATTATGTAAACATTTATTTGTTATTTTGCTTTCTCCTggag   >  2:265500/1‑149 (MQ=255)
                                                                                                                                                    aaCACGCCCGTTGTTTTATTTATGTGGATATTATTAATAGCATATCGAGCATATTTATATGAAGCCCATTACTTGAGCCCATATGGTCATATTTTTATAATGCAACTATTATGTAAACATTTATTTGTTATTTTGCTTTCTCCTggagg  <  2:820206/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
ATGGAAGGCGCAATCAATGCCAAGACCGTAACTTATGACTTCGAACGTCTGATGGAAGGCGCTAAGCTGCTGAAATGTTCAGAGTTTGGTGAAGCGATCATCGAAAACATGTAATCTCTCCATGTGTTAAATATTGAAACGGGCGTATAACACGCCCGTTGTTTTATTTATGTGGATATTATTAATAGCATATCGAGCATATTTATATGAAGCCCATTACTTGAGCCCATATGGGCATATTTTTATAATGCAACTATTATGTAAACATTTATTTGTTATTTTGCTTTCTCCTGGAGG  >  NC_000913/1211464‑1211760

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: