Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,661,884 T→C 9.6% H443H (CAT→CAC)  ynfF → S‑ and N‑oxide reductase, A subunit, periplasmic

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,661,8840TC 9.6% 110.6 / 3.8 43H443H (CAT→CAC) ynfFS‑ and N‑oxide reductase, A subunit, periplasmic
Reads supporting (aligned to +/- strand):  ref base T (19/19);  new base C (2/2);  total (21/22)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.35e-01

CCGTTCTCACCGGCAACGTCGGCATAAACGGCGGCAACTCAGGCGTACGCGAAGGTAGCTGGGATCTGGGGGTAGAATGGTTCCCGATGCTCGAGAATCCTGTTAAAACGCAGATTTCCGTCTTTACATGGACAGATGCCATCGACCATGGTACGGAAATGACCGCGACCCGTGATGGTGTTCGTGGAAAAGAAAAACTGGATGTCCCCATCAAGTTTTTATGGTGCTACGCCAGTAACACATTGATCAATCAACATGGCGACATCAATCACACCCATGAGGTGCTTCAGGATGACA  >  NC_000913/1661736‑1662032
                                                                                                                                                    |                                                                                                                                                    
tgcttCGCACCGGCTACGTCGGCAAAAACCGCGGACACACAGGCGTACCGGAAGTTACCAGGGTTCTGGGGGTAGAATGGTTCCCGATGCTCGAGAATCCTGTTAAAACGCAGATTTCCGTCTTTACATGGACAGATGCCATCGACCAt                                                                                                                                                      <  2:884854/146‑1 (MQ=255)
     ctcACCGGCAACGTCGGCATAAACGGCGGCAACTCCGGCGTACGCGAACGTAGCTGGGAGCTGGGGGTAGATTGGTTCCCGATGCTCGAGAATCCTGTTAAAACGCAGATTTCCGTCTTTACATGGACAGATGCCATCGACCATGGTAc                                                                                                                                                 <  2:179955/149‑1 (MQ=255)
       cACCTGCAACGTCGGCATAAACGGCGGCAACTCCGGCGGACGCGAAGGTAGCTGGGATCTGGGGGTAGAATGGTTCCCGATGCTCGAGAATCCTGTTAAAACGCAGATTTCCGTCTTTACATGGACAGATGCCATCGACCATGGTACgg                                                                                                                                               <  1:659288/149‑1 (MQ=255)
        aCCGGCAACGTCGGCATAAACGGCGGCAACTCAGGCGTACGCGAAGGTAGCTGGGATCTGGGGGTAGAATGGTTCCCGATGCTCGAGAATCCTGTTAAAACGCAGATTTCCGTCTTTACATGGACAGATGCCATCGACCATGGTACGGa                                                                                                                                              >  1:948401/1‑149 (MQ=255)
         ccGGCAACGTCGGCATAAACGGCGGCAACTCAGGCGTACGCGAAGGTAGCTGGGATCTGGGGGTAGATTGGTTCCCGATGCTCGAGAATCCTGTTAAAACGCAGATTTCCGTCTTTACATGGACAGATGCCATCGACCATGGTACGGaa                                                                                                                                             <  2:958726/149‑1 (MQ=255)
           gccAACGTCGGCATAACCGGCGGCAACTCAGGCGTACGCGAAGGTAGCTGGGAACTGGGGGTAGAATGGTTCCCGATGCTCGAGAATCCTGTTAAAACGCAGATATCCGTCTATACATGGACAGATGCCATCGACCATGGTACGGAAAt                                                                                                                                           <  1:908014/147‑1 (MQ=255)
                   cGGCATAAACGGCGGCAACTCACGCGTACGCGAAGGTAGCTGGGATCGGGGGGTAGAATGGATCCCGATGCGCGAGAATCCTGTTAAAACGCAGATTTCCGTCTTTACATCGACAGATGCCATCGACCATGGTACGGAAATGACCGCGa                                                                                                                                   <  2:515967/149‑1 (MQ=255)
                            cggcggCAACTCAGGCGTACGCGAAGGTAGCTGGGATCTGGGGGTAGAATGGTTCCCGATGCTCGAGAATCCTGTTAAAACGCAGATTTCCGTCTTTACATGGACAGATGCCATCGACCACGGTACGGAAATGACCGCGACCCGTGATg                                                                                                                          >  1:158671/1‑149 (MQ=255)
                            cggcggCAACTCAGGCGTACGCGAAGGTAGCTGGGATCTGGGGGTAGAATGGTTCCAGATGCTCGAGAATCCTGTTAAAACGCAGATTTCCGTCTTTACATGGACAGATGCCATCGACCACGGTACGGAAATGACCGCGACCCGTGATg                                                                                                                          >  1:154910/1‑149 (MQ=255)
                                tgcAACTCAGGCGTACGCGAAGGTAGCTGGGATCTGGGGGTAGAATGGTTCCCGATGCTCGAGAATCCTGTTAAAACGCAGATTTCCGTCTTTACATGGACAGATCCCATCGCCCATCGTACGCAAATGACCGCGACCCGTGATGgtgt                                                                                                                      >  1:999107/2‑149 (MQ=255)
                                  cAACTCAGGCGCACGCGACGGTAGCTGGGAGCTGGGGGTAGACTGGATCCCGATGCTCGAGAATCCTGTTAAAACGCAGATTTCCGTCTTTACATGGACAGATGCCATCGACCATGGTACGGAAATGACCGCGACCCGTGATGGTGTTc                                                                                                                    <  2:165241/149‑1 (MQ=255)
                                    aCTCCGGCGTACGCGAAGGTAGCGGGGATCTGGGGGTAGAATGGTTCCCGATGCTCGAGAATCCTGTTAAAACGCAGATTTCCGTCTTTACATGGACAGATGCCATCGACCATGGTACGGAAATGACCGCGACCCGTGATGGTGTTCGt                                                                                                                  <  2:380568/149‑1 (MQ=255)
                                          gCGTACGCGAAGGTAGCTGGGATCTGGGGTTTGAATGGATCCCGATGCTCGAGAATCCTGTTAAAACGCAGATTTCCGTCTTTACATGGACAGATGCCATCGACCATGGTACGGAAATGACCGCGACCCGTGATGGTGTTCGTGgaaaa                                                                                                            <  2:279013/149‑1 (MQ=255)
                                               cgcgAAGGTAGCTGGGATCTGGGGGTAGAATGGTTCCCGATGCTCGAGAATCCTGTTAAAACGCAGATTTCCGTCTTTACAAGGACAGATGCCATCGAGCATGGTACGCAAATGACCGCTACCCGTGATCGTGAGCGTGgaaaaaacaa                                                                                                       >  1:708122/1‑144 (MQ=255)
                                                  gAAGGTAGCTGGGATCTGGGGGTAGAATGGTTCCCGATGCTCGAGAATCCTGTTAAAACGCAGATTTCCGTCTTTACATGGACAGATGCCATCGACCATGGTACGGAAATGACCGCGACCCGTGATGGTGTTCGTGGAAAAGAAAAACt                                                                                                    >  1:302319/1‑149 (MQ=255)
                                                      gTAGGTGGGATCTGGGGGTAGAATGGTTCCCGATGCTCGAGAATCCTGTTAAAAGGCAGATTTCCGTCTTTACATGGACTGATGCCATCGACCATGGTACGGAAATGACCGCGACCCGTGATGGTGTTCGTGGAAAAGAAAAACTGGAt                                                                                                <  1:397149/149‑1 (MQ=255)
                                                      gTAGCTGGGATCTGGGGGTAGAATGGTTCCCGATGCTCGAGAATCCTGTTAAAACGCAGATTTCCGTCTTTACATGGACAGATGCCATCGACCATGGTACGGAAATGACCGCGACCCGTGATCGTGTTCGTGGAAAAGAAAAACTGGAt                                                                                                >  2:407959/1‑149 (MQ=255)
                                                          tggggATCTGGCGTTAGAATGGATCTCGATGCTCGTTAATCCTGTTTATAGGAAGTTTTAGGTCTTTACAATGTCAGTTGCCATCGACGATGGTACGGAAATGACCGCGACCCGTGTTTTTTTTCTTGGAATAGAAAAACTGGAGGTcc                                                                                            <  2:885512/147‑1 (MQ=255)
                                                             ggATCTGGGGGTAGAATGGGGCCCGATGCGCGAGAATCCTGTTAAAACGCAGATTACCGTCTTTACATGGACAGATGCCATCGACCAAGGTACGGAAATGACCGCGACCCGTGATGGTGTTCGTGGAAAAGAAAAACTGGATGTCCCCa                                                                                         <  1:84587/149‑1 (MQ=255)
                                                                    ggggTAGAATGGTTCCCGATGCTCGAGAATCCTGTTAAAACGCAGATTTCCGTCTTTACATGGACAGATGCCATCGACCATGGTACGGAAATGACCGCGACCCGTGATGGTGTTCGTGGAAAAGAAAAACTGGATGTCCCCATCAAGtt                                                                                  >  2:796550/1‑149 (MQ=255)
                                                                      ggTAGAATGGTTCCCGATGCTCGAGAATCCTGTTAAAACGCAGATTTCCGTCTTTACATGGACAGATGCCATCGACCATGGTACGGAAATGACAGCGACCCGTGATGGGGTTCTTGGAATAGAAAAACTGGATGTCCCCATCAAGtttt                                                                                >  1:762438/1‑149 (MQ=255)
                                                                       gtagtaTGGTTCCCGATGATCGAGAATCCTGTTAAAACGCAGATTTCCGTCTTTACATGGACAGATGCCATCGACCATGGTACGGAAATGACCGCGACCCGTGATGGTGTTCGTGGAAAAGAAAAACTGGATGTCCCCATCAAGttttt                                                                               <  1:449455/149‑1 (MQ=255)
                                                                       gTAGAATGGTTCCCGATGCTCGAGAATCCTGTTAAAACGCAGATTTCCGTCTTTACATGGACAGATGCCATCGACCATGGTACGGAAATGACCGCGACCCGTGATGGTGTTCGTGGAAAAGAAAAACTGGCTGTACCCATCAAGatttt                                                                               >  2:570123/1‑149 (MQ=255)
                                                                        tAGAATGGTTCCCGATGCGCGAGAACCCTGTTAAAACGCAGATTTCCGTCTTTACATGGACAGATGCCATAGACCATGGTACGGAAATGACCGCGACCCGTGATGGTGTTCGTGGAAAAGAAAAACTGGATGGCCCCATCAAGTTTTTa                                                                              <  1:450132/149‑1 (MQ=255)
                                                                             tGGTTCCCGATGCTCGAGAATCCTGTTAAAACGCAGATTTCCGTCTTTACATGGACAGATGCCATCGACCACGGTACGGAAATGACCGCGACCCGTGATGGTGTTCGTGGAAAAGAAAAACTGGATGTCCCCATCAAGTTTTTATGGTg                                                                         <  2:154910/149‑1 (MQ=255)
                                                                             tGGGTCCCGATGCTCGAGAATCCTGTTAAAACGCAGATTTGCGTCTTTCCATGGACAGATGCCATCGACCACGGTACGGAAATGACCGCGACCCGTGATGGTGTTCGTGGAAAAGAAAAACTGGATGTCCCCATCAAGTTTTTATGGTg                                                                         <  2:158671/149‑1 (MQ=255)
                                                                                    cGATGCTCGAGAATCCTGTTAAAACGCAGATTTCCGTCTTTACATGGACAGATGCCATCGACCATGGTACGGAAATGACCGCGACCCGTGATGGTGTTCGTGGAAAAGAAAAACTGGATGTCCCCATCAAGTTTTTATGGTGCTACGcc                                                                  >  2:261981/1‑149 (MQ=255)
                                                                                      aTGCTCGAGAATCCTGTTAAAACGCAGATTTCCGTCTTTACATGGACAGATGCCATCGACCATGGTACGGAAATGACCGCGACCCGTGATGGTGTTCGTGGAAAAGAAAAACTGGATGTCCCCATCAAGTTTTTATGGTGCTACGCCAg                                                                <  2:513819/149‑1 (MQ=255)
                                                                                            gagaATCCTGTTAAAACGCAGATTTCCGTCTTTACATGGACAGATGCCATCGACCATGGTACGGAAATGACCGCGACCCGTGATGGTGTTCGTGGAAAAGAAAAACTGGATGTCCCCATCAAGTTTTTATGGTGCTACGCCAGTAacac                                                          >  1:525614/1‑149 (MQ=255)
                                                                                                aTCCTGTTAAAACGCAGATTTCCGTCTTTACATGGACAGATGCCATCGACCATGGTACGGAAATGACCGCGACCCGTGATGGTGTTCGTGGAAAAGAAAAACTGGATGTCCCCATCAAGTTTTTATGGTGCTACGCCAGTAACACATTg                                                      <  2:948401/149‑1 (MQ=255)
                                                                                                 tCCTGTTAAAACGCAGATTTCCGTCTTTACATGGACAGATGCCATCGACCATGGTACGGAAATGACCGCGACCCGTGATGGTGTTCGTGGAAAAGAAAAACTGGATGTCCCCATCAAGTTTTTATGGTGCTACGCCAGTAACACATTGa                                                     >  2:885260/1‑149 (MQ=255)
                                                                                                    tGTTAAAACGCAGATTTCCGTCTTTAAATGGACAGATGCCATCGACCGTGGTACGGAAATGACCGCGACCCGTGATGGTGTTCGTGTAATAGAAAACCTGGATGTCCCCATCTAGTGTTTATGGTGCTACGACAGTAACACATTGatca                                                  >  2:238575/1‑149 (MQ=255)
                                                                                                       tAAAACGCAGATTTCCGTCTTTACATGGACAGATGCCATCGACCATGGTACGGAAATGACCGCGACCCGTGATGGTGTTCGTGGAAAAGAAAAACTGGATGTCCCCATCAAGTTTTTATGGTGCTACGCCAGTAACACATTGatcaatc                                               <  1:319590/149‑1 (MQ=255)
                                                                                                         aaaCGCAGATTTCCGTCTTTACATGGACAGATGCCATCGACCATGGTACGGAAATGACCGCGACCCGTTATGGTGTTAGTGGAAATGAAAAACTGGATGTCCCCATCAAGATATTATGGTGCTACGCCAGTAACACATTGAtctatcaa                                             >  1:418424/1‑149 (MQ=255)
                                                                                                          aaCGCAGATTTCCGTCTTTACTTGGACAGATGCCATCGACCATGGTACGGAAATGACCGCGACCCGTGATGGTGTTCGTGGAATAGAAAAACTGGATGTCCCCATCAAGTTTTTATGGTGCTACGCCAGTAACACATTGATCAATCAAc                                            <  2:15819/149‑1 (MQ=255)
                                                                                                          aaCGCAGATTTCCGTCTTTACATGGACAGATGCCATCGACCATGGTACGGAAATGACCGCGACCCGTGATGGTGTTCGTGGAAAAGAAAAACTGGATGTCCCCATCAAGTTTTTATGGTGCTACGCCAGTAACACATTGATCAATCAAc                                            <  1:796550/149‑1 (MQ=255)
                                                                                                              aaGATTTCAGGTTTTTCTTGGACATATTACATCTACGATTGTACTGTAATGACCCCGCCCCATGATGGTGTTCGAGGAAAAGACAAACTGGATGTCCCCATCAAGTTTTTATGGTGCTCCGCCAGTAACACATTGATAAATCAACATgg                                        <  2:102317/148‑1 (MQ=255)
                                                                                                                      cGTCTTTACATGGACAGATGCCATCGACCATGGTACGGAAATGCCCGCGACCCGTGATGGTGTTCGTGGAAAAGAAAAACTGGTTGTCCCCATCAAGTTTTTATGGTGCTACGCCAGTATCACATTGATCAATCCAAATGGCGACatca                                >  2:896174/1‑149 (MQ=255)
                                                                                                                          tttACATGGACAGATGCCATAGACCATGGTACGGAAATGACCGCGACCCGTGATGGTGGTCGTGGAAAAGAAAAACTGGATGTCCCCCTCAAGTTTTTATGGTGCTAAGCCAGTACCACATTGATCAATCAACATGGCGACatcactcc                            >  1:9146/1‑148 (MQ=255)
                                                                                                                                     aGATGCCATCGACCATGGTACGGAAATGACCGCGCCCCGTGATGGTGTTCGTGGAAAAGAAAAACTGGATGTCCCCATCAAGTTTTTATGGTGCTACGCCAGGAACACATTGATCAATCAACATGGCGACATCAATCACACCCATGAgg                 >  1:843908/1‑149 (MQ=255)
                                                                                                                                         gCCATCGACCATGGTACGGAAATGACCGCGACCCGTGATGGTGTTCGTGGAAAAGAAAAACTGGATGTCCCCATCAAGTTTTTATGGTGCTACGCCAGTAACACATTGATCAATCAACATGGCGACATCAATCACACCCATGAGGTGCt             >  1:656956/1‑149 (MQ=255)
                                                                                                                                           cATCGACCATGGTACGGAAATGACCGCGCCCCGTGATGGTGTTCGTGGAAAAGAAAAACTGGATGTCCCCATCAAGTTTTTATGGTGCTACGCCAGTAACACATTGATCAATCAACATGGCGACATCAATCACACCCATGAGGTGCTTc           >  1:752223/1‑149 (MQ=255)
                                                                                                                                           cATCGACCATGGTACGGAAATGACCGCGACCCGTGATGGTGTTCGTGGAAAAGAAAAACTGGATGTCCCCATCAAGTTTTTATGGTGCTACGCCAGTAACACATTGATCAATCAACATGGCGACATCAATCACACCCATGAGGTGCTTc           >  1:409242/1‑149 (MQ=255)
                                                                                                                                            aTCGACCATGGTACGGAAATGACCGCGACCCGTGATGGTGTTCGTGGAAAAGAAAAACTGGATGTCCCCATCAAGTTTTTATGGTGCTACGCCAGTAACACATTGATCAATCAACATGGCGACATCAATCACACCCATGAGGTGCTTCa          <  2:656956/149‑1 (MQ=255)
                                                                                                                                                    tGGTACGGAAATGACCGCGACCCGTGATGGTGTTCGTGGAAAAGAAAAACTGGATGTCCCCATCAAGTTTTTATGGTGCTACGCCAGTAACACATTGATCAATCAACATGGCGACATCAATCACACCCATGAGGTGCTTCAGGATGACa  <  2:525614/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
CCGTTCTCACCGGCAACGTCGGCATAAACGGCGGCAACTCAGGCGTACGCGAAGGTAGCTGGGATCTGGGGGTAGAATGGTTCCCGATGCTCGAGAATCCTGTTAAAACGCAGATTTCCGTCTTTACATGGACAGATGCCATCGACCATGGTACGGAAATGACCGCGACCCGTGATGGTGTTCGTGGAAAAGAAAAACTGGATGTCCCCATCAAGTTTTTATGGTGCTACGCCAGTAACACATTGATCAATCAACATGGCGACATCAATCACACCCATGAGGTGCTTCAGGATGACA  >  NC_000913/1661736‑1662032

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑