| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 1,674,955 | A→T | 11.5% | intergenic (+8/+17) | tqsA → / ← pntB | pheromone AI‑2 transporter/pyridine nucleotide transhydrogenase, beta subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,674,955 | 0 | A | T | 11.5% | 106.1 / 4.7 | 49 | intergenic (+8/+17) | tqsA/pntB | pheromone AI‑2 transporter/pyridine nucleotide transhydrogenase, beta subunit |
| Reads supporting (aligned to +/- strand): ref base A (27/15); new base T (2/4); total (29/20) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.97e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTATGCTGCTTTCCGTGCCGTTGACAATTATTGTCAAAATTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTGGGTGTTTTCCTTGAAGAACAGCGGGTTTTGCACACCAGCATAGCCAGTGTTCATCGAACGTTTAAAGACAATCAC > NC_000913/1674807‑1675103 | ttGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTATGCTGCTTTCCGTGCCGTTGACAATTATTGTCAAAATTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTca > 1:175635/1‑149 (MQ=255) tGATTTTTTGGGGATTGTTGTTAGGTCCTGTGGTTAGGCGGCTTTACGTGCCGTTGACAATTATTGTCAATATTGCGCTTGAACCAACAGCGGGAGGGCAAAGCATCGCCGTGCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTcag < 1:606357/149‑1 (MQ=255) ttttttGGGGATGGTTGTTAGGACCGGTGGGTATGCTGCTTTCCGTGCCGTTGACAATTATTGTCAAAATTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCATcagcag > 1:1021546/1‑149 (MQ=255) ttttGGGGATGGTTGTTAGGACCGGTGGGTATGCTGCTTTCCGTGCCGTTGACAATTATTGTCAAAATTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGCGTGACGGCCTcagccgcg > 1:350893/1‑145 (MQ=255) gggATGGTTGTTAGGACCGGGGGGTATGCTGCTTTCCGTGCCGTTGACAATTATTGTCAAAATTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCTGCTGAGGCCGt < 1:37997/149‑1 (MQ=255) ggATGGTTGTTAGGACCGGGGGGTATGCTGCTTTCCGTGCCGTTGACAATTATTGTCAAAATTGCGCTGGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAAAAAAGAGTGACGGCCTCTGCTGAGGCCGTc < 1:746779/149‑1 (MQ=255) aTGGTTGTGAGGACCGGGGGGTATGCTGCTTTCCGTGCCGTAGACAATTATTGTCAAAATTGCGCTTGAACAAACAGCGGGAGGGCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCTGCTGAGGCCGTCac < 1:259892/149‑2 (MQ=255) tgttATGACCGGGGGGTATGCTGCTTTCCGGGCCGTTGACAATTATTGGAAAAATTGCGCTTGAACAAACAGCGGGATGTCAAAGCATGGCCGTTCTGTTAATCGATCTCAATAAAGCGTGACGGCCTCAGCAGATGCCGTCATGGTTa < 2:962849/149‑1 (MQ=255) aGGACCGGTGGGTATGCTGCTTTCCGTGCCGTTGACAATTATTGTCAAAATAGCGCTGGAACACACAGCGGGAGGGCAAAGAATCGCCGTTCTGTTAAGCGATCTCATTAAAGAGTGACGGCCTCAGACGAGGCCCTCAGGGTTACaga > 2:582923/1‑149 (MQ=255) gACCGGTGGGTATGCTGCTTTCCGTGCCGTTGACAATTATTGTCAAAATTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGc > 1:498126/1‑149 (MQ=255) ccGGTGGGTATGCTGCTTTCCGTGCCGTTGACAATTATTGTCAAAATTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCtt > 2:497640/1‑149 (MQ=255) ctgctTTCCGTGCCGTTGACAATTATTGTCAAAATTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCGGAGGCCGTCAGGGTTACAGCGCTTTCAGGATTGCAt > 1:822363/1‑149 (MQ=255) tttCCGTGCCGTTGACAATTATTGTCAAAATTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCAc > 1:363296/1‑149 (MQ=255) tCCGTGCCGTTGACAATTATTGTCAAAATTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGc < 2:175635/149‑1 (MQ=255) gTGCCGTTGACAATTATTGTCAAAATTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGGTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCGTTCAGGATTGCATCCACGCTgg > 2:29859/1‑149 (MQ=255) tGCCGTTGACAATTATTGTCAAAATTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAAGATTGCATCCACGCTGGc > 2:907009/1‑149 (MQ=255) tCAAAATTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAg > 1:67282/1‑149 (MQ=255) aaaTTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCAt < 1:497640/149‑1 (MQ=255) aaTTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATAGCCGTTCAGATAAGCGATCTCAATAAAGAATGAAGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGAATCCATCCAAGCAGGCTTTGGCGTCACCAAAcatcatc > 1:732163/1‑148 (MQ=255) gcTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACTTCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTGGgtg > 1:473556/1‑149 (MQ=255) aacaGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTGGGTGTTTTCCTTg > 1:800558/1‑149 (MQ=255) acaGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTGGGTGGTTTCCTTga > 1:543350/1‑149 (MQ=255) caGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTAACATATCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTAACCAAAAAGCATGTGCGTTTTTTCCTTgaa > 1:605915/1‑149 (MQ=255) aGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTGGGTGTTTTCCTTgaag > 2:730455/1‑149 (MQ=255) cGGGAGGTCAAAGCATCGCCGTTCTGTTAATCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCATGGTTACAGAGCTTTCAGGATTTCCTCCACGCTGGCTTTGGCGTCACCAAACAGCATGTGGTTGTGTTCCTTgaagaa > 2:480621/1‑149 (MQ=255) aGCATGGCCGGTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTGGGTGTTTTCCTTGAAGAACAGCGGGtttt < 1:131147/149‑1 (MQ=255) aGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCAGCAAACAGCATGTGGGTGTTTTCCTTGAAGATCAGCGGGtttt > 2:535392/1‑149 (MQ=255) cATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTGGGTGTTTTCCTTGAAGAACAGCGGGTTTTGc < 1:827616/149‑1 (MQ=255) cATCGCCGTGCTGTTAAGCGATAGCAATAATGCGTGACTGCCTCAGCAGAGGCCGTCAGGGTTCCAGCGCTTTCAGGATTGCATCCACGCGGGCTTTGGCGTCACCAAACAGCATGTGGGTGTTTTCCTTGAAGAACAGCGGGTTTTGc < 1:738042/149‑1 (MQ=255) agcGCCGGTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCCGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTGGGTGTTTTCCTTGAAGAACAGCGGGTTTTGca < 2:350893/147‑1 (MQ=255) aTCGCCGTTCTGTTAAGCGATCTCAATAAAGAGGGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTGGGTGTTTTCCTTGAAGAACAGCGGGTTTTGca < 1:398276/149‑1 (MQ=255) tCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTGGGTGTTTTCCTTGAAGAACAGCGGGTTTTGcac > 1:6050/1‑149 (MQ=255) gTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGGTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTGGGTGTTTTCCTTGAACAACAGCGGGTTTTGCTCACCCGCagag > 1:51497/1‑149 (MQ=255) ttAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGGCGTCAGGGGTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTGGGTGTTTTCCTTGAAGAACAGCGGGTTTTGCACACCAGCATAGc < 2:435363/149‑1 (MQ=255) aaGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCCCCAACCAGCATGTGGGTGTTTTCCTTCAAGAACAGCGGGTTTTGCACACCCGCATAGCCa > 1:143138/1‑149 (MQ=255) aGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTGGGTGTTTTCCTTGAAGAACAGCGGGTTTTGCACACCAGCATAGCCAg < 1:730455/149‑1 (MQ=255) aGCGAGCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTGGGTGTTTTCCTTGAAGAACAGCGGGTTTTGCACACCAGCATAGCCAg < 2:67282/149‑1 (MQ=255) cGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTGGGTGTTTTCTTTGAAGCACAGCGGGTTTTGCACACCAGCATAGCCAgtg > 2:248799/1‑149 (MQ=255) aTCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTGGGTGTTTTCCTTGAAGAACAGCGGGTTTTGCACACCAGCATAGCCAGTGtt < 2:363296/149‑1 (MQ=255) tcAATAAAGCGGGTCGGCCTCTTCAGAGGCCGTCAGGGTTTCAGAGCTTTCATGATTGCATCCACGCTGGCTTTGGCGTCACCAATCAGCATTTGGGTGTTTTCCTTGAAGAACAGCGGGTTTTGCACACCAGCATAGCCAGTGTTCAt < 2:732163/149‑1 (MQ=255) cAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTGGGTGTTTTCCTTGAAGAACAGCGGGTTTTGCACACCAGCATAGCCAGTGTTCATc > 1:4118/1‑149 (MQ=255) aaTAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGTCGTCACCAAACAGCATGTGGGTGTTTTCCTTGAAGAACAGCGGGTTTTGCACACCAGCATAGCCAGTGTTAATCg > 2:356707/1‑149 (MQ=255) tAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTGGGTGTTTTCCTTGAAGAACAGCGGGTTTTGCACACCAGCATAGCCAGTGTTCATCGaa > 1:340817/1‑149 (MQ=255) gagTGACGGTCTCAGCAGAGTCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCAGTCTTGCTTTGGCGTCACCAAACAGCATGTTTGTGTTTTCCTTGAAGAACAGCGGGTTTTGCACACCAGCATAGCCAGTGTTCATCGAACGtt < 1:258017/149‑1 (MQ=255) cctgccGGCCTCTGCTGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTGTGGCGTCGCCCACCAGCATGTGGGTGTTTTCCTTGTAGATAAGCGGGTTTTGCTCACCAGCCTCGCCAGTGTTCATCGAACGttt > 1:691088/6‑149 (MQ=255) ctGACGGCCTCTGCTGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTGGGTGTTTTCCTTGAAGAACAGCGGGTTTTGCACACCAGCATAGCCAGTGTTCATCGAACGTTTa > 1:170655/2‑149 (MQ=255) gACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTGGGTGTTTTCCTTGAAGAACAGCGGGTTTTGCACACCAGCATAGCCAGTGTTCATCGAACGTTTaaa < 1:385673/149‑1 (MQ=255) aCGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTGGGTGTTTTCCTTGAAGAACAGCGGGTTTTGCACACCAGCATAGCCAGTGTTCATCGAACGTTTaa > 2:578351/1‑147 (MQ=255) aCGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTGGGTGTTTTCCTTGAAGAACAGCGGGTTTTGCACACCAGCATAGCCAGTGTTCATCGAACGTTTaa < 1:578351/147‑1 (MQ=255) aCGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTGGGTGTTTTCCTGGAAGAACAGCGGGGTTTGCACACCCGCATAGCCAGTGTTGATCGCACGTTTAAAg > 2:180616/1‑149 (MQ=255) cGGCCGCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCGGGCTTTGGCGTCACCAAACAGCATGTGGGTGTTTTCCTTGAAGAACAGCGGGTGTTGCACACCAGCATAGCCAGTGTTCATCGAACGTTTAAAGa < 1:726073/149‑1 (MQ=255) ccTCAGCAGAGGCCGTCAGCGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTGGGTGTTTTCCTTGAAGAACAGCGGGTTTTGCACACCAGCATAGCCAGTGGTCCTCGCACGTTTACAGACaa > 2:613895/1‑149 (MQ=255) cTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTACATCCACGCTGGCTTTGGCGTCACCAAACAGCTTGTGGGTGTTTTCCTTGAAGAACAGCGGGTTTTGCACACCAGCATAGCCAGTGTTCATCGAACGTTTAAAGACAAt > 1:309658/1‑149 (MQ=255) cagcagAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTGGTTGTTTTCCTTGAAGAACAGCGGGTTTTGCACACCAGCATAGCCAGTGTTCATCGAACGTTTAAAGACAATCa < 2:1021546/149‑1 (MQ=255) cagcagAGGCCGTCAGGGCTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTGGGTGTTTTCCTTGAAGAACAGCGGGTTTTGCACACCAGCATAGCCAGTGTTCATCGAACGTTTAAAGACAATCa > 1:1007459/1‑149 (MQ=255) agcagAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTGGGTGTTTTCCTTGAAGAACAGCGGGTTTTGCACACCAGCATAGCCAGTGTTCATCGAACGTTTAAAGACAATCAc > 2:384798/1‑149 (MQ=255) | TTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTATGCTGCTTTCCGTGCCGTTGACAATTATTGTCAAAATTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTGGGTGTTTTCCTTGAAGAACAGCGGGTTTTGCACACCAGCATAGCCAGTGTTCATCGAACGTTTAAAGACAATCAC > NC_000913/1674807‑1675103 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |