Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,842,780 G→A 9.1% G137E (GGA→GAA)  gdhA → glutamate dehydrogenase, NADP‑specific

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,842,7800GA 9.1% 102.3 / 3.2 44G137E (GGA→GAA) gdhAglutamate dehydrogenase, NADP‑specific
Reads supporting (aligned to +/- strand):  ref base G (21/19);  new base A (2/2);  total (23/21)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.35e-01

GCCCGTACAAAGGCGGTATGCGCTTCCATCCGTCAGTTAACCTTTCCATTCTCAAATTCCTCGGCTTTGAACAAACCTTCAAAAATGCCCTGACTACTCTGCCGATGGGCGGTGGTAAAGGCGGCAGCGATTTCGATCCGAAAGGAAAAAGCGAAGGTGAAGTGATGCGTTTTTGCCAGGCGCTGATGACTGAACTGTATCGCCACCTGGGCGCGGATACCGACGTTCCGGCAGGTGATATCGGGGTTGGTGGTCGTGAAGTCGGCTTTATGGCGGGGATGATGAAA  >  NC_000913/1842636‑1842922
                                                                                                                                                |                                                                                                                                              
gCCCGTACAAAGGCGGTATGCGCTTCCCTCCGTCAGTTAACCTTTCCATTCTCAAATTCCTCGGCTTTGAACAAACCTTCAAAAATGCCCTGACTACTCTGCCGATGGGCGCTGGTAAAGGCGGCAGCGATTTCGATCCGATAGGaaaa                                                                                                                                            >  2:189809/1‑149 (MQ=255)
 cccGTACAAAGGCGGTATGCGCTTCCATCCGTCAGTTAACCTTTCCATTCTCAAATTCCTCGGCTTTGAACAAACCTGCAAAAATGCCCTGACTACTCTGCCGATGGGCGGTGGTAAAGGCGGCAGCGATTTCGATCCGAAAGGaaaaa                                                                                                                                           >  1:17073/1‑149 (MQ=255)
  ccGTACAAAGGCGGTATGCGCTTCCATCCGTCAGTTAACCTTTCCATTCTCAAATTCCTCGGCTTTGAACAAACCTTCAAAAATGCCCTGACTACTCTGCCGATGGGCGGTGGTAAAGGCGGCAGCGATTTCGATCCGAAAGAAAAAAg                                                                                                                                          >  1:96531/1‑149 (MQ=255)
  ccGTACAAAGGCGGTATGCGCTTCCATCCGTCAGTTAACCTTNCCATTCTCAAATTCCTCGGCTTTGAACAAACCATCAAAAATGCCCTGACTACTCTGCCGATGGGCGGTGGTAAAGGCGGCAGCGATTTCGATCCGAAAGAAAAAAg                                                                                                                                          >  1:100157/1‑149 (MQ=255)
      aCAACGGCGGGATGCGCTTCCATCCGTCAGTTAACCTTTCCATTCTCAAATTCCTCGGCTTTGAACAAACCTTCAAAAATGCCCTGACTACTCTGCCGATGGGCGGTGGTAAAGGCGGCAGCGATTTCGATCCGAAAGGAAAAAGCGaa                                                                                                                                      <  2:594862/149‑1 (MQ=255)
      aCAAAGGCGGTATGCGCTTCCATCCGTCAGTTAACCTTTCCATTCTCAAATTCCTCGGCTTTGAACACACCTTCAAAAATGCCCTGACTACTCTGCCGATGGGCGGTGGTAAAGGCGGCAGCGATTTCGATCCGAAAGGAAAAAGCGat                                                                                                                                      >  1:332258/1‑148 (MQ=255)
        aaaGGCGGTATGCGCTTCCATCCGTCAGTTAACCTTTCCATTCTCAAATTCCTCGGCTTTGAACAAACCTTCAAAAATGCCCTGACTACTCTGCCGATGGGCGGTGGTAAAGGCGGCAGCGATTTCGATCCGAAAGGAAAAAGCGAAgg                                                                                                                                    >  2:383946/1‑149 (MQ=255)
                 aTGCGCTTCCATCCGTCAGTTAACCTTTCCATTCTCAAATTCCTCGGCTTTGAACAAACCTTCAAAAATGCCCTGACTACTCTGCCGATGGGCGGTGGTAAAGGCGGCAGCGATTTCGATCCGAAAGGAAAAAGCGAAGGTGAAGTGAt                                                                                                                           <  2:894042/149‑1 (MQ=255)
                  tGCGCTTCCATCCGTCAGTTAACCTTTCCATTCTCAATTTCCTCGGCTTTGAACAAACCTTCAAAAATGCCCTGACTACTCTGCCGATGGGCGGTGGTAAAGGCGGCAGCGATTTCGATCCGAAAGGAAAAAGCGAAGGTGAAGTGATg                                                                                                                          >  2:589421/1‑149 (MQ=255)
                         ccATCCGTCAGTTAACCTTTCCATTCTCAAATTCCTCGGCTTTGAACAAACCTTCAAAAATGCCCTGACTACTCTGCCGATGGGCGGTGGTAAAGGCGGCAGCGATTTCGATCCGAAAGGAAAAAGCGAAGGTGAAGTGATGCGttttt                                                                                                                   <  2:311709/149‑1 (MQ=255)
                            tCCCTCATTTAACCTTTCCATTCTCAAATTCCTCTGCTTTGAACAAACCTTCAAAAATGCCCTGACTACTCTGCCGATGGGCGGTGGTAAAGGCGGCAGCGATTTCGATCCGAAAGGAAAAAGCGAAGGTGAAGTGATGCGTTTTTGcc                                                                                                                <  2:687833/149‑1 (MQ=255)
                                   gTTAACCTTTCCATTCTCAAATTCCTCGGCTTTGAACAAACCTTCAAAAATGCCCTGACTACTCTGCCGATGGGCGGTGGTAAAGGCGGCAGCGATTTCGATCCGAAAGGAAAAAGCGAAGGTGAACTGATGCGTTTTTGCCAGGCGCt                                                                                                         >  2:897471/1‑149 (MQ=255)
                                      aaCCTTTCCATTCTCAAATTCCTCGGCGTTGAACAAACCTTCAAAAATGCCCTGAGTACTCTGCCGATGGGCGGTGGTAAAGGCGGCAGCGATTTCGATCCGAAAGGAAAAAGCGAAGGTGAAGTGATGCGTTTTTGCCAGGCGCtgat                                                                                                      <  2:192968/149‑1 (MQ=255)
                                      aaCCTTTCCATTCTCAAATACCTCGGCTGTGAACAAACCTGCAAAACTGCCCTGACTACTCTGCGGATGTGCGGTGGTAACGGCGGCAGCGATTTCGATCCGAAAGGAAACAGCGAAGGTGACGTGATGCGTTTTTGCCAGGCGCtgat                                                                                                      <  2:922773/149‑1 (MQ=255)
                                        ccTTTCCATTCTCAAATTCTTCGTATTTGAACAAACCTTGAAAAATTCCCAGACTACTCTGCCTATGGGCGGTGGTTAAGGCGGCAGCGATTTCGATCCGAATGGAAAAAGCGAAGGTTAAGTGATGCGTTTTTGCCAGGCGCtgatga                                                                                                    <  1:256787/149‑1 (MQ=255)
                                        ccTTTCCATTCTCAAATTCTTCGGCTTTGAACAAACCTTCAATAATGCCCTGACTACTCTGCCGATGGGCGGTGGTATAGGCGGCAGCGATTTCGATCCGAAAGGAAATAGCGAAGGTGAAGTGATGCGTTTTTGCCAGGCGCtgatga                                                                                                    <  2:695263/149‑1 (MQ=255)
                                        ccTTTCCATTCTCAAATTCCTCGGCTTTGAACAAACCTTCAAAAATGCCCTGACTACTCTGCCGATGGGCGGTGGTAAAGGCGGCAGCGATTTCGATCCGAAAGGAAAAAGCGAAGGTGAAGTGATGCGTTTTTGCCAGGCGCtgatga                                                                                                    <  1:114723/149‑1 (MQ=255)
                                                  ctcAAATTCCTCGGCTTTGAACAAACCTTCAAAAATGCCCTGACTACTCTGCCGATGGGCGGGGGTAAAGGCGGCAGCGATTTCGATCCGAAAGGAAAAAGCGAAGGTGAAGTGATGCGTTTTTGCCAGGCGCTGATGACTGAACTGTa                                                                                          <  1:315469/149‑1 (MQ=255)
                                                   tcAAATTCCTCGGCTTTGAACCAACCTTCAAAAATGCCCTGACTACTCTGCCGATGGGCGGAGGTACAGGCGGCAGCGATTTCGAACCGAAAGGAAAAAGCGAAGGAGAAGTGTTGGGTTGTTGCGAGGCGCGGATGCCTGAACTGTAt                                                                                         >  2:967820/1‑149 (MQ=255)
                                                      aaTTCCTCGGCTTTGAACAAACCTTCAAAAATGCCCTGACTACTCTGCCGATGGGCGGTGGTAAAGGCGGCAGCGATTTCGATCCGAAAGGAAAAAGCGAAGGTGAAGTGATGCGTTTTTGCCAGGCGCTGATGACTGAACTGTATCGc                                                                                      >  1:448480/1‑149 (MQ=255)
                                                         tCCTCGGCTTTGAACAAACCTTCAAAAATGCCCTGACTACTCTGCCGATGGGCGGTGGTAAAGGCGGCAGCGATTTCGATCCGAAAGGAAAAAGCGAAGGTGAAGTGATGCGTTTTTGCCAGGCGCTGATGACTGAACTGTATCGCCAc                                                                                   <  2:828048/149‑1 (MQ=255)
                                                                 tttGAACAAACCTTCAAAAATGCCCTGACTACTCTGCCGATGGGCGGTGGTAAAGGCGGCAGCTATTTCGATCCGAAAGGAAAAAGCGAAGGTGAAGTGATGCGTTTTTGCCAGGCGCTGATGACTGAACTGTATCGCCACCTGGgcgc                                                                           >  1:855667/1‑149 (MQ=255)
                                                                    gAACAACCCTTCAAAAATGCCCTGACTACTCTGCCGATGGGCGGTGGTAAAGGCGGCAGCGATTTCGATCCGAAAGGAAAAAGCGAAGGTGAAGTGATGCGTTTTTGCCAGGCGCTGATGACTGAACTGTATCGCCACCTGGGCGCGGa                                                                        >  2:542448/1‑149 (MQ=255)
                                                                     aaCAAACCTTCAAAAATGCCCTGACTACTCTGCCGATGGGCGGTGGTAAAGGCGGCAGCGATTTCGATCCGAAAGGAAAAAGCGAAGGTGAAGTGATGCGTTTTTGCCAGGCGCTGGCGACTGAAc                                                                                              <  2:254795/126‑1 (MQ=255)
                                                                     aaCAAACCTTCAAAAATGCCCTGACTACTCTGCCGATGGGCGGTGGTAAAGGCGGCAGCGATTTCGATCCGAAAGGAAAAAGCGAAGGTGAAGTGATGCGTTTTTGCCAGGCGCTGGAGACTGAAc                                                                                              >  1:254795/1‑126 (MQ=255)
                                                                     aaCAAACCTTCAAAAATGCCCTGACTACTCTGCCGATGGGCGGTGGTAAAGGCGGCAGCGATTTCGATCCGAAAGGAAAAAGCGAAGGTGAAGTGATGCGTTTTTGCCAGGCGCTGATGACTGAACTGTATCGCCCCCTGGGCGCGGAt                                                                       >  1:180869/1‑149 (MQ=255)
                                                                            cTTCAAAAATGCCCTGACTACTCTGCCGATGGGCGGTGGTAAAGGCGGCAGCGATTTCGATCCGAAAGGAAAAAGCGAAGGTGAAGTGATGCGTTTTTGCCAGGCGCTGATGCCTGAACTGTATCGCCACCTGGGCGCGGATACCGACg                                                                >  2:515350/1‑149 (MQ=255)
                                                                               cAAAAATGCCCTGACTACTCTGCCGATGGGCGGTGGTAAAGGCGGCAGCGATTTCGATCCGAAAGGAAAAAGCGAAGGTGAAGTGATGCGTTTTTGCCAGGCGCTGATGACTGAACTGTATCGCCACCTGGGCGCGGATACAGACGTTc                                                             >  1:102257/1‑149 (MQ=255)
                                                                                aaacaggCCCGGACTCCTCTGCCGAGCGGCGGTGGTAAAGGCGGCAGCGAGTTCGATCCGAAAGGAACCAGCGAAGGGGAAGTGATGCGTGTTTGCCAGGAGCTGATGACTGAACTGTATCGCCACCTGGGCGCGGAAACCGACGTTcc                                                            <  1:1024096/143‑1 (MQ=255)
                                                                                aaaaaTGCCCTGACTACTCTGCCGATGGGCGGTGGTAAAGGCGGCAGCGATTTCGATCCGAAAGGAAAAAGCGAAGGTGAAGTGATGCGTTTTTGCCAGGCGCTGATGACTGAACTGTATCGCCACCTGGGCGCGGATACCGACGTTcc                                                            >  1:865363/1‑149 (MQ=255)
                                                                                aaaaaTGCCCTGACTACTCTGCCGATGGGCGGTGGTAAAGGCGGCAGCGATTTCGATCCGAAAGGAAAAAGCGAAGGTGAAGTGATGCGTTTTTGCCAGGCGCTGATGACTGAACTGTATCGCCACCTGGGCGCGGATACCGACGTTcc                                                            >  1:861309/1‑149 (MQ=255)
                                                                                aaaaaTGCCCTGACTACTCTGCCGATGGGCGGTGGTAAAGGCGGCAGCGATTTCGATCCGAAAGAAAAAAGCGAAGGTGAAGTGATGCGTTTTTGCCAGGCGCTGATGACTGAACTGTATCGCCACCTGGGCGCGGATACCGACGTTcc                                                            <  2:100157/149‑1 (MQ=255)
                                                                                aaaaaTGCCCTGACTACTCTGCCGATGGGCGGTGGTAAAGGCGGCAGCGATTTCGATCCGAAAGAAAAAAGCGAAGGTGAAGTGATGCGTTTTTGCCAGGCGCTGATGACTGAACTGTATCGCCACCTGGGCGCGGATACCGACGTTcc                                                            <  2:96531/149‑1 (MQ=255)
                                                                                 aaaaTGCCCTGACTACTCTGCCGATGGGCGGTTGGAAAGGCGTCAGCGATTTCGATCCGAAAGGAAAAAGCGAAGGTGAAGTGATGCGTTTTTGCCAGGCGCTGATGACTGAACTGTATCGCCACCTGGGCGCGGATACCGACGTTCCg                                                           <  2:651506/149‑1 (MQ=255)
                                                                                  aaaTGCCCTGACTACTCTGCCGATGGGCGGTGGTAAAGGCGGCAGCGATTTCGATCCGAAAGGAAAAAGCGAAGGTGAAGTGATGCGTTTTTGCAAGGCGCTGATGACTGAACTGTATCGCAACCTGGGCGCGTATACCGACTTTCCgg                                                          >  2:938855/1‑149 (MQ=255)
                                                                                                ctctGCCGATTGGCGGGTGTAAAGGCGGCAGCGATTGAGATCCGACAGGAAAAAGCGAACGTGAATTGATGCTTTTTTGCCAGGCGCTGATGACTGAACTGTATCGCCACCTGGGCGCGGATACCGACGTTCCGGCAGGTGATATCggg                                            <  2:374123/149‑1 (MQ=255)
                                                                                                     ccGATGGGCGGTGGTAAAGGCGGCAGCGATTTCGATCCGAAAGGAAAAAGCGAAGGTGAAGTGATGCGTTTTTGCCAGGCGCTGATGACTGAACTGTATCGCCACCTGGGCGCGGATACCGACGTTCCGGCAGGTGATATCGGGGTtgg                                       >  2:279596/1‑149 (MQ=255)
                                                                                                     ccGATGGGCGGTGGTAAAGGCGGCAGCGATTTCGATCCGAAAGGAAAAAGCGAAGGTGAAGTGATGCGTTTTTGCCAGGCGCTGATGACTGAACTGTATCGCCACCTGGGCGCGGATACCGACGTTCCGGCAGGTGATATCGGGGTtgg                                       <  1:897471/149‑1 (MQ=255)
                                                                                                      cGATGGGCGGTGGTAAAGGCGGCAGCGATTTCGATCCGAAAGGAAAAAGCGAAGGTGAAGTGCTGCGTTTTTGCCAGGCGCTGATGACTGAACTGTATCGCCACCTGGGCGCGGATACCGACGTTCCGGCAGGTGATATCGGGGTtggt                                      >  1:992799/1‑149 (MQ=255)
                                                                                                      cGATGGGCGGTGGTAAAGGCGGCAGCGATTTCGATCCGAAAGGAAAAAGCGAAGGTGAAGTGATGCGTTTTTGCCAGGCGCTGATGACTGAACTGTATCGCCACCTGGGCGCGGATACCGACGTTCCGGCAGGTGATATCGGGGTtggt                                      <  1:845194/149‑1 (MQ=255)
                                                                                                             cGGTGGTAAAGGCGGCAGCGATTTCGATCCGAAAGGAAAAAGCGAAGGTGAAGTGATGCGTTTTTGCCCTGCGCTGATGACTGAACTGTATCGCCACCTGGGCGCGTATACCGACGTTCCGGCAGGTGATATCGGGGTTGGTGGTCGTg                               <  1:589421/149‑1 (MQ=255)
                                                                                                                                gATTTCGATCCGAAAGGAAAAAGCGAAGGTGAAGTGATGCGTTTTTGCCAGGCGCTGATGACTGTACTGTATCGCCACCTGGGCGCGGATACCGACGTTCCGGCAGGTGATATCGGGGTTGGTGGTCGTGAAGTCGGCTTTATGGCggg            <  2:17073/149‑1 (MQ=255)
                                                                                                                                gATTTCGATCCGAAAGGAAAAAGCGAAGGTGAAGTGATGCGTTTTTGCCAGGCGCTGATGACTGAACTGTATCGCCACCTGGGCGCGGATACCGACGTTCCGGCAGGTGATATCGGGGTTGGTGGTCGTGAAGTCTGCTTTATGGCggg            >  1:514471/1‑149 (MQ=255)
                                                                                                                                          cGAAAGGAAAAAGCGAAGGTGAAGTGCTGCGTTTTTGCCAGGCGCTGATGACTGAACTGTATCGCCACCTGGGCGCGGATACCGCCGTTCCGGCAGGTGATATCGGGTTGGGTGGTCGTGAAGTCGGCTTTATGGCGGGGATGATGaaa  >  2:122390/1‑149 (MQ=255)
                                                                                                                                                |                                                                                                                                              
GCCCGTACAAAGGCGGTATGCGCTTCCATCCGTCAGTTAACCTTTCCATTCTCAAATTCCTCGGCTTTGAACAAACCTTCAAAAATGCCCTGACTACTCTGCCGATGGGCGGTGGTAAAGGCGGCAGCGATTTCGATCCGAAAGGAAAAAGCGAAGGTGAAGTGATGCGTTTTTGCCAGGCGCTGATGACTGAACTGTATCGCCACCTGGGCGCGGATACCGACGTTCCGGCAGGTGATATCGGGGTTGGTGGTCGTGAAGTCGGCTTTATGGCGGGGATGATGAAA  >  NC_000913/1842636‑1842922

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑