Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,896,471 T→G 8.2% L101R (CTG→CGG)  nudL → putative CoA pyrophosphohydrolase, weak 3‑phosphohydroxypyruvate phosphatase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,896,4710TG 8.2% 120.9 / 2.5 49L101R (CTG→CGG) nudLputative CoA pyrophosphohydrolase, weak 3‑phosphohydroxypyruvate phosphatase
Reads supporting (aligned to +/- strand):  ref base T (28/17);  new base G (2/2);  total (30/19)
Fisher's exact test for biased strand distribution p-value = 6.36e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.95e-01

ATCTGCGTAAACACGCTGGACAAGTGGCATTCCCTGGAGGTGCAGTCGATGACACGGACGCATCAGCTATCGCCGCCGCGCTGCGCGAAGCTGAAGAAGAGGTCGCTATACCGCCTTCCGCCGTTGAAGTTATCGGCGTGCTGCCGCCCGTCGATAGCGTCACTGGCTACCAGGTAACCCCAGTGGTCGGCATTATCCCGCCCGATCTGCCGTATCGCGCCAGTGAAGATGAAGTCTCGGCGGTGTTTGAAATGCCGCTCGCCCAGGCATTACATCTGGGTCGTTAT  >  NC_000913/1896330‑1896616
                                                                                                                                             |                                                                                                                                                 
aTCTGCGTAAACACGCTGGACAAGTGGCATTCCCTGGAGGTGCAGTCGATGACACGGACGCATCAGCTATCGCCGCCGCGCTGCGCGAAGCTGAAGAAGAGGTCGCTATACCGCCTTCCGCCGTTGAAGTTATCGGCGTGCTGCCGccc                                                                                                                                            >  1:629886/1‑149 (MQ=255)
 tCTGCGTAAACACGCTGGACAAGTGGCATTCCCTGGAGGTGCAGTCGATGACACGGACGCATCAGCTATCGCCGCCGCGCTGCGCGAAGCTGAAGAAGAGGTCGCTATACCGCCTTCCGCCGTTGAAGTTATCGGCGTGCTGCCGCCCg                                                                                                                                           >  2:85220/1‑149 (MQ=255)
   tGCGTAAACACGCTGGACAAGTGGCATTCCCTGGAGGTGCAGTCGATGACACGGACGCATCAGCTATCGCCGCCGCGCTGCGCGAAGCTGAAGAAGAGGTCGCTATACCGCCTTCCGCCGTTGAAGTTATCGGCGTGCTGCCGCCCGTc                                                                                                                                         >  2:250540/1‑149 (MQ=255)
   tGCGTAAACACGCTGGACAAGTGGCATTCCCTGGAGGTGCAGTCGATGACACGGACGCATCAGCTATAGCCGCCGCGCTGCGCGAAGCTGAAGAAGAGGTCGCTATACCGCCTTCCGCCGTTGAAGTTATCGGCGTGCTGCCGCCCGTc                                                                                                                                         >  2:750818/1‑149 (MQ=255)
      gTAAACACGCTGGACAAGTGGCATTCCCTGGAGGTGCAGTCGATGACACGGACGCATCAGCTATCGCCGCCGCGCTGCGCGAAGCTGAAGAAGAGGTCGCTATACCGCCTTCCGCCGTTGAAGTTATCGGCGTGCTGCCGCCCGTCGAt                                                                                                                                      >  1:295673/1‑149 (MQ=255)
       tAAACACGCTGGACAAGTGGCATTCCCTGGAGGTGCAGTCGATGACACGGACGCATCAGCTATCGCCGCCGCGCTGCGCGAAGCTGAAGAAGAGGTCGCTATACCGCCTTCCGCCGTTGAAGTTATCGGCGTGCTGCCGCCCGTCGATa                                                                                                                                     <  2:112483/149‑1 (MQ=255)
        aaaCACGCTGGACAAGTGGCATTCCCTGGAGGTGCAGTCGATGACACGGACGCATCAGCTATCGCCGCCGCGCTGCGCGAAGCTGAAGAAGAGGTCGCTATACCGCCTTCCGCCGTTGAAGTTATCGGCGTGCTGCCGCCCGTCGATAg                                                                                                                                    >  1:31025/1‑149 (MQ=255)
         aaCACGCTGGACAAGTGGCATTCCCTGGAGGTGCAGTCGATGTCACGGACGCATCAGCTATCGCCGCCGCGCTGCGCGAAGCTGAAGAAGAGGTCGCTATACCGCCTTCCGCCGTTGAAGTTATCGGCGTGCTGCCGCCCGTCGATAGc                                                                                                                                   <  2:1007987/149‑1 (MQ=255)
          acacGCTGGACAAGGGGCACTCCCTGGAGGGGCAGGCGAAGACACGGACGCATCAGCTATCGCCGCCGCGCTGCGCGAAGCTGAAGAAGAGGTCGCTATACCGCCTTCCGCCGTTGAAGTTATCGACGGGCGGCAGCACGCCGATaact                                                                                                                                  <  1:276392/149‑4 (MQ=255)
           cacGCTGGACAAGTGGCATTCCCTGGAGGTGCAGTCGATGACACGGACGCATCAGCTATCGCCGCCGCGCTGCGCGAAGCTGAAGAAGAGGTCGCTATACCGCCTTCCGCCGTTGAAGTTATCGGCGTGCTGCCGCCCGTCGATAGCGt                                                                                                                                 >  1:153580/1‑149 (MQ=255)
              gCTGGACAAGTGGCATTCCCTGGAGGTGCAGTCGATGACACGGACGCATCAGCTATCGCCGCCGCGCTGCGCGAAGCTGAAGAAGAGGTCGCTATACCGCCTTCCGCCGTTGAAGTTATCGGCGTGCTGCCGCCCGTCGATAGCGTCAc                                                                                                                              >  2:771042/1‑149 (MQ=255)
              gCTGGACAAGTGGCATTCCCTGGAGGTGCAGTCGATGACACGGACGCATCAGCTATCGCCGCCGCGCTGCGCGAAGCTGAAGAAGAGGTCGCTATACCGCCTTCCGCCGTTGAAGTTATCGGCGTGCTGCCGCCCGTCGATAGCGTCAc                                                                                                                              <  2:339603/149‑1 (MQ=255)
              gCTGGACAACTCCCAATCCCTGGCGCTGCAGCCGATCCCACGGACGCATCAGCTCCCGCCGCCGCGCTGCGCGAAGCTGAAGAAGAGGTCGCTAAACCGCCTTCCGCCGTTGAAGATATCGACGGGCGGCAGCACGCCGATAACTTCaa                                                                                                                              <  1:146891/149‑2 (MQ=255)
               cTGGACAAGTGGCATTCCCTGGAGGTGCAGTCGATGACACGGACGCATCAGCTATCGCCGCCGCGCTGCGCGAAGCTGAAGAAGAGGTCGCTATACCGCCTTCCGCCGTTGAAGTTATCGGCGTGCTGCCGCCCGTCGATAGCGTCACt                                                                                                                             >  2:214257/1‑149 (MQ=255)
                 ggACAAGTGGCATTCCCTGGAGGTGCAGTCGATGACACGGACGCATCAGCTATCGCCGCCGCGCTGCGCGAAGCTGAAGAAGAGGTCGCTATACCGCCTTCCGCCGTTGAAGTTATCTGCGTGCTGCCGCCCGTCGATAGCGTCACTgg                                                                                                                           <  2:378971/149‑1 (MQ=255)
                     aaGTGGCATTCCCTGGAGGTGCAGTCGATGACACGGACGCATCAGCTATCGCCGCCGCGCTGCGCGCAGCTGAAGCAGAGGTCGCTATACCGCCTTCCGCCGTTGAAGTTATCGGCGTGCTGCCGCCCGGCTATAGCCTCACTGGCTAc                                                                                                                       >  2:201411/1‑149 (MQ=255)
                      aGTGGCATTCCCTGGAGGTGCAGTCGATGACACGGACGCATCAGCTATCGCCGCCGCGCTGCGCGAAGCTGAAGAAGAGGTCGCTATACCGCCTTCCGCCGTTGAAGTTATCGGCGTGCTGCCGCCCGTCGATAGCGTCACTGGCTAcc                                                                                                                      <  2:629886/149‑1 (MQ=255)
                             ttCCCTGGAGGTGCAGTCGATGACACGGACGCATCAGCTATCGCCGCCGCGCTGCGCGAAGCTGAAGAAGAGGTCGCTATACCGCCTTCCGCCGTTGAAGTTATCGGCGTGCTGCCGCCCATCGATAGCGTCACTGGCTACCAGGTAAc                                                                                                               >  1:281951/1‑149 (MQ=255)
                              tCCCTGGAGGTGCAGTCGATGACACGGACGCATCAGCTATCGCCGCCGCGCTGCGCGAAGCTGAAGAAGAGGTCGCTATACCGCCTTCCGCCGTTGAAGTTATCGGCGTGCTGCCGCCCGGCGATAGCGTCACTGGCTACCAGGTAAcc                                                                                                              <  1:58436/149‑1 (MQ=255)
                               cccTGGAGGTGCAGTCGATGACACGGACGCATCAGCTATCGCCGCCGCGCTGCGCGAAGCTGAAGAAGAGGTCGCTATACCGCCTTCCGCCGTTGAAGTTATCGGCGTGCTGCCGCCCGTCGATAGCGTCACTGGCTACCAGGTAAccc                                                                                                             <  2:448049/149‑1 (MQ=255)
                                   gcaGGTGCAGTCGATGACACGGACGCATCAGCGATCGCCGCCGCGCTGCGCGAAGCTTAAGAAGAGGTAGCTAGACCGCCTTCCTCCGGCGAAGTTATCGGCGTGCTGCCGCCCGTCGATAGCGTCACTGGCTACCAGGTAACCCCAGt                                                                                                         <  2:173062/147‑1 (MQ=255)
                                           aGTCGATGACACGGACGCATCAGCTATCGCCGCCGCGCTGCGCGAAGCTGAAGAAGAGGTCGCTATACCGCCTTCCGCCGTTGAAGTTATCGGCGTGCTGCCGCCCGTCGATAGCGTCACTGGCTACCAGGTAACCCCAGTGGTCGGCa                                                                                                 >  1:547541/1‑149 (MQ=255)
                                             tCGATGACACGGACGCATCAGCTATCGCCGCCGCGCTGCGCGAAGCTGAAGAAGAGGTCGCTATACCGCCTTCCGCCGTTGAAGTTATCGGCGTGCTGCCGCCCGTCGATAGCGTCACTGGCTACCAGGTAACCCCAGTGGTCGGCAtt                                                                                               >  2:225670/1‑149 (MQ=255)
                                               gATGACACGGACGCATCAGCTATCGCCGCCGCGCTGCGCGAAGCTGAAGAAGAGGTCGCTATACCGCCTTCCGCCGTTGAAGTTATCGGCGTGCTGCGGCCCGTCGATAGCGTCACTGGCTACCAGGTAACCCCAGTGGTCGGCCTTAt                                                                                             >  1:1022887/1‑149 (MQ=255)
                                                aTGACACGGACGCATCAGCTATCGCCGCCGCGCTGCGCGAAGCTGAAGAAGAGGTCGCTATACCGCCTTGCGCCGTTGAAGTTATCGGCGTGCTGCCGCCCGTCGATAGCGTCACTGGCTACCGGGTAACCCCAGTGGTCGGCATTATc                                                                                            >  2:803451/1‑149 (MQ=255)
                                                     acGGACGCATCAGCTATCGCCGCCGCGCTGCGCGAAGCTGAAGAAGAGGTCGCTATACCGCCTTCCGCCGTTGAAGTTATCGGCGTGCTGCCGCCCGTCGATAGCGTCACTGGCTACCAGGTAACCCCAGTGGTCGGCATTATcccgcc                                                                                       >  1:918141/1‑149 (MQ=255)
                                                     acGGACGCATCAGCTATCGCCGCCGCGCTGCGCGAAGCTGAAGAAGAGGTCGCTATACCGCCTTCCGCCGTTGAAGTTATCGGCGTGCTGCCGCCCGTCGATAGCGTCACTGGCTACCAGGTAACCCCAGTGGTCGGCATTATcccgcc                                                                                       >  2:39662/1‑149 (MQ=255)
                                                              tCAGCTATCGCCGCCGCGCTGCGCGAAGCTGAAGAAGAGGTCGCTATACCGCCTTCCGCCGTTGAAGTTATCGGCGTGCTGCCGCCCGTCGATAGCGTCTCTGGCTACCAGGTCACCCCAGTGGTCGGCATTATCCCGCCCGATCTGcc                                                                              >  1:27976/1‑149 (MQ=255)
                                                                aGCAATCGCCGCCGCGCTGCGCGAAGCTGAAGAAGAGGTCGCTATACCGCCTTCCGCCGTTGAAGTTATCGGCGTGCTGCAGCCCGTCGAGCGCGTCACTGGCTACCAGGTAACCCCCGTGGTCGCCAATATCCCGCCCGATCTGCCGt                                                                            >  2:51364/1‑149 (MQ=255)
                                                                     tCGCCGCCGCGCTGCGCGAAGCTGAAGAAGAGGTCGCTATACCGCCTTCCGCCGTTGAAGTTATCGGCGTGCTGCCGCCCGTCGATAGCGTCACTGGCTACCAGGTAACCCCAGTGGTCGGCCTTATCCCGCCCGATCTGCCGTATcgc                                                                       >  1:134813/1‑149 (MQ=255)
                                                                       gccgccgcGCTGCGCGAAGCTGAAGAAGAGGTCGCTATACCGCCTTCCGCCGTTGAAGTTATCGGCGTGCTGCCGCCCGTCGATAGCGTCACTGGCTACCAGGTAACCCCAGTGGTCGGCATTATCCCGCCCGATCTGCCGTATCgcgc                                                                     <  2:765920/149‑1 (MQ=255)
                                                                        ccgccgcGCTGCGCGAAGCTGAAGAAGAGGTCGCTATACCGCCTTCCGCCGTTGAAGTTATCGGCGTGCTGCCGCCCGTCGATAGCGTCACTGGCTACCAGGTAACCCCAGTGGTCGGCATTATCCCGCCCGATCTGCCGTATCGCGcc                                                                    >  1:12887/1‑149 (MQ=255)
                                                                              cgcTGCTCGAAGCTGAAGAAGAGTGCGCTATACCGCCTTCCGCCGTTGAAGTTATCGGCGTGCTGCCGCCCGTCGATAGCGTCACTGGCTACCAGGTAACCCCAGTGGTCGGCATTATCCCGCCCGATCTGCCGTATCGCGCCAGTGaa                                                              <  2:59620/149‑1 (MQ=255)
                                                                                    gcgAAGCTGAAGAAGAGGTCGCTATACCGCCTTCCGCCGTTGAAGTTATCGGCGTGCTGCCGCCCGTCGATAGCGTCACTGGCTACCAGGTAACCCAAGTGGTCGGCATTATCCCGCCCGATCTGCAGTATCGCGCCAGTTAAGATGaa                                                        >  1:553580/1‑149 (MQ=255)
                                                                                           tGAAGAAGAGGTCGCTATACCGCCTTCCGCCGTTGAAGTTATCGGCGTGCTGCCGCCCGTCGATAGCGTCACTGGCTACCAGGTAACCCCAGTGGTCGGCATTATCCCGCCCGATCTGCCGTATCGCGCCAGTGAAGATGAAGTCTcgg                                                 >  1:712227/1‑149 (MQ=255)
                                                                                            gaagaaGAGGTCGCTATACCGCCTTCCGCCGTTGAAGTTATCGGCGTGCTGCCGCCCGTCGATGGCGTCACTGGCTACCAGGAAACCCCAGTGGTCGGCATTATCCCGCCCGCTCTGCCGGATCGCGCCAGTGAAGATGAAGGCTcggg                                                >  1:1003582/1‑148 (MQ=255)
                                                                                                 agagGTCGCTATACCGCCTTCCGCCGTTGATGTTATCGGCGTGCTGCCGCCCGTCGATAGCGTCACTGGCTACCAGGTAACCCCAGTGGTCGGCATTATCCCGCCCGATCTGCCGTATCGCGCCAGTGAAGATGAAGTCTCGGCGgtgt                                           <  2:604855/149‑1 (MQ=255)
                                                                                                  gagGTCGCTATACCGCCTTCCGCCGTTGAAGTTATCGGCGTGCTGCCGCCCGTGGATAGCGTCACTGGCTACCAGGTAACCCCAGTGGTCGGCATTATCCCGCCCGATCTGCCGTATCGCGCCAGTGAAGATGAAGTCTCGGCGGTGtt                                          <  1:214257/149‑1 (MQ=255)
                                                                                                       cGCTATACCGCCTTCCGCCGTTGACGTTATCGGCGTGCTGCCGCCCGGCGATAGCGTCACTGGCTACCAGGTAACCCCAGTGGTCGGCATTATCCCGCCCGATCTGCCGTATCGCGCCAGTGAAGATGAAGTCTCGGCGGTGTTTGaaa                                     <  1:968399/149‑1 (MQ=255)
                                                                                                              ccGCCTTCCGCCGTTGAAGTTATCGGCGTGCTGCCGCCCGTCGATAGCGTCACTGGCTACCAGGTAACCCCAGTGGTCGGCATTATCCCGCCCGATCTGCCGTATCGCGCCAGTGAAGATGAAGTCTCGGCGGTGTTTGAAATGCCGCt                              >  1:234197/1‑149 (MQ=255)
                                                                                                                    tCCGCCGTTGAAGTTATCGGCGTGCTGCCGCCCGTCGTTAGCGTCACTGGCTACCAGGTAACCCCAGTGGTCGGCATTATCCCGCCCGATCTGCCGTATCGCGCGAGTGATGATGAAGTCTCGGCGGTGTTTGAAATGCCGCTCGCCCa                        <  1:250540/149‑1 (MQ=255)
                                                                                                                          gTTGAAGTTATCGGCGTGCTGCCGCCCGTCGATAGCGTCACTGGCTACCAGGTAACCCCAGTGGTCGGCATTATCCCGCCCGATCTGCCGTATCGCGCCAGTGAAGATGAAGTCTCGGCGGTGTTTGAAATGCCGCTCGCCCAGGCAtt                  <  2:918141/149‑1 (MQ=255)
                                                                                                                           ttGAAGTTATCGGCGTGCTGCCGCCCGTCGATAGCGTCACTGGCTACCCGGTACCCCCAGTGGTCGGCATTATCCCGCCCGATCTGCCGTATCGCGCCAGGGATGATGAAGTCTCGGCGGTGTTTGAACTGCCGCTCGCCCAGGCAtgc                 >  1:495963/1‑147 (MQ=255)
                                                                                                                            tGAAGTTATCGGCGTGCTGCCGCCCGTCGATAGCGTCACTGGCTACCAGGTAACCCCAGTGGTCGGCAGTATCCCGCCCGATCTGCCGTATCGCGCCAGTGAAGATGAAGTCTCGGCGGTGTTTGAAATGCCGCTCGCCCAGGCATTAc                <  1:85220/149‑1 (MQ=255)
                                                                                                                                 ctATCGACGGGCGGCAGCACGCCGATAGCGTCACTGGCTACCAGGTAACCCCAGTGGTCGGCATTATCCCGCCCGATCTGCCGTATCGCGCCAGTGAAGATGAAGTCTCGGCGGTGTTTGAAATGCCGCTCGCCCAGGCATTACATCTg           >  2:503332/2‑149 (MQ=255)
                                                                                                                                   aTCGGCGTGCTGCCGCCCGTCGATAGCGTCACTGGCTACCAGGTAACCCCAGTGGTCGGCATTATCCCGCCCGATCTGCCGTATCGCGCCAGTGAAGATGAAGTCTCGGCGGTGTTTGCAATGCCGCTCGCCCAGGCAGTACATCTggg         >  1:940317/1‑149 (MQ=255)
                                                                                                                                   aTCGACGGGCGGCAGCACGCCGATAGCGTCACTGGCTACCAGGAAACACCAGTGGTCGGCATTATCCCGCCCGATCTGCCGTATCGCCCCAGTGAAGATGAAGTCTCGGCGGGGTTTGAAATGCCGCTCGCCCAGGAATTACATGTggg         >  1:183124/1‑149 (MQ=255)
                                                                                                                                       gCGTGCTGCCTCCCGTCGATAGCGTCACTGGCTACCAGGTAACCCCAGTGGTCGGCATTATCCCGCCCGATCTGCCGTATCGCGCCAGTGAAGATGAAGTCTCGGCGGTGTTTGTAATGCCGCTCGCCCAGGCATTACATCTGGGTCGt     <  2:586661/149‑1 (MQ=255)
                                                                                                                                       gCGTGCTGCCGCCCGTCGATAGCGTCACTGGCTACCAGGTAACCCCAGTGGTCGGCATTATCCCGCCCGATCTGCCGTATCGCGCCAGTGAAGCTGAAGTCTCGGCGGTGTTTGAAATGCCGCTCGCCCAGGCATTACATCTGGGTCGt     >  1:376848/1‑149 (MQ=255)
                                                                                                                                          tgctgcCGCCCGTCGATAGCGTCACTGGCTACCGGGTAACCCCAGTGGTCGGCATTATCCCGCCCGATCTGCCGTATCGCGCCAGTGAAGATGAAGTCTCGGCGGTGTTTGAAATGCCGCTCGCCCAGGCATTACATCTGGGTCGTTAt  <  1:803451/149‑1 (MQ=255)
                                                                                                                                             |                                                                                                                                                 
ATCTGCGTAAACACGCTGGACAAGTGGCATTCCCTGGAGGTGCAGTCGATGACACGGACGCATCAGCTATCGCCGCCGCGCTGCGCGAAGCTGAAGAAGAGGTCGCTATACCGCCTTCCGCCGTTGAAGTTATCGGCGTGCTGCCGCCCGTCGATAGCGTCACTGGCTACCAGGTAACCCCAGTGGTCGGCATTATCCCGCCCGATCTGCCGTATCGCGCCAGTGAAGATGAAGTCTCGGCGGTGTTTGAAATGCCGCTCGCCCAGGCATTACATCTGGGTCGTTAT  >  NC_000913/1896330‑1896616

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑