Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,896,480 T→C 8.2% V104A (GTC→GCC)  nudL → putative CoA pyrophosphohydrolase, weak 3‑phosphohydroxypyruvate phosphatase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,896,4800TC 8.2% 101.1 / 3.5 50V104A (GTC→GCC) nudLputative CoA pyrophosphohydrolase, weak 3‑phosphohydroxypyruvate phosphatase
Reads supporting (aligned to +/- strand):  ref base T (25/18);  new base C (2/2);  total (28/22)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.63e-01

TGCGTAAACACGCTGGACAAGTGGCATTCCCTGGAGGTGCAGTCGATGACACGGACGCATCAGCTATCGCCGCCGCGCTGCGCGAAGCTGAAGAAGAGGTCGCTATACCGCCTTCCGCCGTTGAAGTTATCGGCGTGCTGCCGCCCGTCGATAGCGTCACTGGCTACCAGGTAACCCCAGTGGTCGGCATTATCCCGCCCGATCTGCCGTATCGCGCCAGTGAAGATGAAGTCTCGGCGGTGTTTGAAATGCCGCTCGCCCAGGCATTACATCTGGGTCGTTATCACCCTTTAGA  >  NC_000913/1896333‑1896627
                                                                                                                                                   |                                                                                                                                                   
tGCGTAAACACGCTGGACAAGTGGCATTCCCTGGAGGTGCAGTCGATGACACGGACGCATCAGCTATCGCCGCCGCGCTGCGCGAAGCTGAAGAAGAGGTCGCTATACCGCCTTCCGCCGTTGAAGTTATCGGCGTGCTGCCGCCCGTc                                                                                                                                                    >  2:250540/1‑149 (MQ=255)
tGCGTAAACACGCTGGACAAGTGGCATTCCCTGGAGGTGCAGTCGATGACACGGACGCATCAGCTATAGCCGCCGCGCTGCGCGAAGCTGAAGAAGAGGTCGCTATACCGCCTTCCGCCGTTGAAGTTATCGGCGTGCTGCCGCCCGTc                                                                                                                                                    >  2:750818/1‑149 (MQ=255)
   gTAAACACGCTGGACAAGTGGCATTCCCTGGAGGTGCAGTCGATGACACGGACGCATCAGCTATCGCCGCCGCGCTGCGCGAAGCTGAAGAAGAGGTCGCTATACCGCCTTCCGCCGTTGAAGTTATCGGCGTGCTGCCGCCCGTCGAt                                                                                                                                                 >  1:295673/1‑149 (MQ=255)
    tAAACACGCTGGACAAGTGGCATTCCCTGGAGGTGCAGTCGATGACACGGACGCATCAGCTATCGCCGCCGCGCTGCGCGAAGCTGAAGAAGAGGTCGCTATACCGCCTTCCGCCGTTGAAGTTATCGGCGTGCTGCCGCCCGTCGATa                                                                                                                                                <  2:112483/149‑1 (MQ=255)
     aaaCACGCTGGACAAGTGGCATTCCCTGGAGGTGCAGTCGATGACACGGACGCATCAGCTATCGCCGCCGCGCTGCGCGAAGCTGAAGAAGAGGTCGCTATACCGCCTTCCGCCGTTGAAGTTATCGGCGTGCTGCCGCCCGTCGATAg                                                                                                                                               >  1:31025/1‑149 (MQ=255)
      aaCACGCTGGACAAGTGGCATTCCCTGGAGGTGCAGTCGATGTCACGGACGCATCAGCTATCGCCGCCGCGCTGCGCGAAGCTGAAGAAGAGGTCGCTATACCGCCTTCCGCCGTTGAAGTTATCGGCGTGCTGCCGCCCGTCGATAGc                                                                                                                                              <  2:1007987/149‑1 (MQ=255)
       acacGCTGGACAAGGGGCACTCCCTGGAGGGGCAGGCGAAGACACGGACGCATCAGCTATCGCCGCCGCGCTGCGCGAAGCTGAAGAAGAGGTCGCTATACCGCCTTCCGCCGTTGAAGTTATCGACGGGCGGCAGCACGCCGATaact                                                                                                                                             <  1:276392/149‑4 (MQ=255)
        cacGCTGGACAAGTGGCATTCCCTGGAGGTGCAGTCGATGACACGGACGCATCAGCTATCGCCGCCGCGCTGCGCGAAGCTGAAGAAGAGGTCGCTATACCGCCTTCCGCCGTTGAAGTTATCGGCGTGCTGCCGCCCGTCGATAGCGt                                                                                                                                            >  1:153580/1‑149 (MQ=255)
           gCTGGACAAGTGGCATTCCCTGGAGGTGCAGTCGATGACACGGACGCATCAGCTATCGCCGCCGCGCTGCGCGAAGCTGAAGAAGAGGTCGCTATACCGCCTTCCGCCGTTGAAGTTATCGGCGTGCTGCCGCCCGTCGATAGCGTCAc                                                                                                                                         <  2:339603/149‑1 (MQ=255)
           gCTGGACAAGTGGCATTCCCTGGAGGTGCAGTCGATGACACGGACGCATCAGCTATCGCCGCCGCGCTGCGCGAAGCTGAAGAAGAGGTCGCTATACCGCCTTCCGCCGTTGAAGTTATCGGCGTGCTGCCGCCCGTCGATAGCGTCAc                                                                                                                                         >  2:771042/1‑149 (MQ=255)
           gCTGGACAACTCCCAATCCCTGGCGCTGCAGCCGATCCCACGGACGCATCAGCTCCCGCCGCCGCGCTGCGCGAAGCTGAAGAAGAGGTCGCTAAACCGCCTTCCGCCGTTGAAGATATCGACGGGCGGCAGCACGCCGATAACTTCaa                                                                                                                                         <  1:146891/149‑2 (MQ=255)
            cTGGACAAGTGGCATTCCCTGGAGGTGCAGTCGATGACACGGACGCATCAGCTATCGCCGCCGCGCTGCGCGAAGCTGAAGAAGAGGTCGCTATACCGCCTTCCGCCGTTGAAGTTATCGGCGTGCTGCCGCCCGTCGATAGCGTCACt                                                                                                                                        >  2:214257/1‑149 (MQ=255)
              ggACAAGTGGCATTCCCTGGAGGTGCAGTCGATGACACGGACGCATCAGCTATCGCCGCCGCGCTGCGCGAAGCTGAAGAAGAGGTCGCTATACCGCCTTCCGCCGTTGAAGTTATCTGCGTGCTGCCGCCCGTCGATAGCGTCACTgg                                                                                                                                      <  2:378971/149‑1 (MQ=255)
                  aaGTGGCATTCCCTGGAGGTGCAGTCGATGACACGGACGCATCAGCTATCGCCGCCGCGCTGCGCGCAGCTGAAGCAGAGGTCGCTATACCGCCTTCCGCCGTTGAAGTTATCGGCGTGCTGCCGCCCGGCTATAGCCTCACTGGCTAc                                                                                                                                  >  2:201411/1‑149 (MQ=255)
                   aGTGGCATTCCCTGGAGGTGCAGTCGATGACACGGACGCATCAGCTATCGCCGCCGCGCTGCGCGAAGCTGAAGAAGAGGTCGCTATACCGCCTTCCGCCGTTGAAGTTATCGGCGTGCTGCCGCCCGTCGATAGCGTCACTGGCTAcc                                                                                                                                 <  2:629886/149‑1 (MQ=255)
                          ttCCCTGGAGGTGCAGTCGATGACACGGACGCATCAGCTATCGCCGCCGCGCTGCGCGAAGCTGAAGAAGAGGTCGCTATACCGCCTTCCGCCGTTGAAGTTATCGGCGTGCTGCCGCCCATCGATAGCGTCACTGGCTACCAGGTAAc                                                                                                                          >  1:281951/1‑149 (MQ=255)
                           tCCCTGGAGGTGCAGTCGATGACACGGACGCATCAGCTATCGCCGCCGCGCTGCGCGAAGCTGAAGAAGAGGTCGCTATACCGCCTTCCGCCGTTGAAGTTATCGGCGTGCTGCCGCCCGGCGATAGCGTCACTGGCTACCAGGTAAcc                                                                                                                         <  1:58436/149‑1 (MQ=255)
                            cccTGGAGGTGCAGTCGATGACACGGACGCATCAGCTATCGCCGCCGCGCTGCGCGAAGCTGAAGAAGAGGTCGCTATACCGCCTTCCGCCGTTGAAGTTATCGGCGTGCTGCCGCCCGTCGATAGCGTCACTGGCTACCAGGTAAccc                                                                                                                        <  2:448049/149‑1 (MQ=255)
                                gcaGGTGCAGTCGATGACACGGACGCATCAGCGATCGCCGCCGCGCTGCGCGAAGCTTAAGAAGAGGTAGCTAGACCGCCTTCCTCCGGCGAAGTTATCGGCGTGCTGCCGCCCGTCGATAGCGTCACTGGCTACCAGGTAACCCCAGt                                                                                                                    <  2:173062/147‑1 (MQ=255)
                                        aGTCGATGACACGGACGCATCAGCTATCGCCGCCGCGCTGCGCGAAGCTGAAGAAGAGGTCGCTATACCGCCTTCCGCCGTTGAAGTTATCGGCGTGCTGCCGCCCGTCGATAGCGTCACTGGCTACCAGGTAACCCCAGTGGTCGGCa                                                                                                            >  1:547541/1‑149 (MQ=255)
                                          tCGATGACACGGACGCATCAGCTATCGCCGCCGCGCTGCGCGAAGCTGAAGAAGAGGTCGCTATACCGCCTTCCGCCGTTGAAGTTATCGGCGTGCTGCCGCCCGTCGATAGCGTCACTGGCTACCAGGTAACCCCAGTGGTCGGCAtt                                                                                                          >  2:225670/1‑149 (MQ=255)
                                            gATGACACGGACGCATCAGCTATCGCCGCCGCGCTGCGCGAAGCTGAAGAAGAGGTCGCTATACCGCCTTCCGCCGTTGAAGTTATCGGCGTGCTGCGGCCCGTCGATAGCGTCACTGGCTACCAGGTAACCCCAGTGGTCGGCCTTAt                                                                                                        >  1:1022887/1‑149 (MQ=255)
                                             aTGACACGGACGCATCAGCTATCGCCGCCGCGCTGCGCGAAGCTGAAGAAGAGGTCGCTATACCGCCTTGCGCCGTTGAAGTTATCGGCGTGCTGCCGCCCGTCGATAGCGTCACTGGCTACCGGGTAACCCCAGTGGTCGGCATTATc                                                                                                       >  2:803451/1‑149 (MQ=255)
                                                  acGGACGCATCAGCTATCGCCGCCGCGCTGCGCGAAGCTGAAGAAGAGGTCGCTATACCGCCTTCCGCCGTTGAAGTTATCGGCGTGCTGCCGCCCGTCGATAGCGTCACTGGCTACCAGGTAACCCCAGTGGTCGGCATTATcccgcc                                                                                                  >  1:918141/1‑149 (MQ=255)
                                                  acGGACGCATCAGCTATCGCCGCCGCGCTGCGCGAAGCTGAAGAAGAGGTCGCTATACCGCCTTCCGCCGTTGAAGTTATCGGCGTGCTGCCGCCCGTCGATAGCGTCACTGGCTACCAGGTAACCCCAGTGGTCGGCATTATcccgcc                                                                                                  >  2:39662/1‑149 (MQ=255)
                                                           tCAGCTATCGCCGCCGCGCTGCGCGAAGCTGAAGAAGAGGTCGCTATACCGCCTTCCGCCGTTGAAGTTATCGGCGTGCTGCCGCCCGTCGATAGCGTCTCTGGCTACCAGGTCACCCCAGTGGTCGGCATTATCCCGCCCGATCTGcc                                                                                         >  1:27976/1‑149 (MQ=255)
                                                             aGCAATCGCCGCCGCGCTGCGCGAAGCTGAAGAAGAGGTCGCTATACCGCCTTCCGCCGTTGAAGTTATCGGCGTGCTGCAGCCCGTCGAGCGCGTCACTGGCTACCAGGTAACCCCCGTGGTCGCCAATATCCCGCCCGATCTGCCGt                                                                                       >  2:51364/1‑149 (MQ=255)
                                                                  tCGCCGCCGCGCTGCGCGAAGCTGAAGAAGAGGTCGCTATACCGCCTTCCGCCGTTGAAGTTATCGGCGTGCTGCCGCCCGTCGATAGCGTCACTGGCTACCAGGTAACCCCAGTGGTCGGCCTTATCCCGCCCGATCTGCCGTATcgc                                                                                  >  1:134813/1‑149 (MQ=255)
                                                                    gccgccgcGCTGCGCGAAGCTGAAGAAGAGGTCGCTATACCGCCTTCCGCCGTTGAAGTTATCGGCGTGCTGCCGCCCGTCGATAGCGTCACTGGCTACCAGGTAACCCCAGTGGTCGGCATTATCCCGCCCGATCTGCCGTATCgcgc                                                                                <  2:765920/149‑1 (MQ=255)
                                                                     ccgccgcGCTGCGCGAAGCTGAAGAAGAGGTCGCTATACCGCCTTCCGCCGTTGAAGTTATCGGCGTGCTGCCGCCCGTCGATAGCGTCACTGGCTACCAGGTAACCCCAGTGGTCGGCATTATCCCGCCCGATCTGCCGTATCGCGcc                                                                               >  1:12887/1‑149 (MQ=255)
                                                                           cgcTGCTCGAAGCTGAAGAAGAGTGCGCTATACCGCCTTCCGCCGTTGAAGTTATCGGCGTGCTGCCGCCCGTCGATAGCGTCACTGGCTACCAGGTAACCCCAGTGGTCGGCATTATCCCGCCCGATCTGCCGTATCGCGCCAGTGaa                                                                         <  2:59620/149‑1 (MQ=255)
                                                                                 gcgAAGCTGAAGAAGAGGTCGCTATACCGCCTTCCGCCGTTGAAGTTATCGGCGTGCTGCCGCCCGTCGATAGCGTCACTGGCTACCAGGTAACCCAAGTGGTCGGCATTATCCCGCCCGATCTGCAGTATCGCGCCAGTTAAGATGaa                                                                   >  1:553580/1‑149 (MQ=255)
                                                                                        tGAAGAAGAGGTCGCTATACCGCCTTCCGCCGTTGAAGTTATCGGCGTGCTGCCGCCCGTCGATAGCGTCACTGGCTACCAGGTAACCCCAGTGGTCGGCATTATCCCGCCCGATCTGCCGTATCGCGCCAGTGAAGATGAAGTCTcgg                                                            >  1:712227/1‑149 (MQ=255)
                                                                                         gaagaaGAGGTCGCTATACCGCCTTCCGCCGTTGAAGTTATCGGCGTGCTGCCGCCCGTCGATGGCGTCACTGGCTACCAGGAAACCCCAGTGGTCGGCATTATCCCGCCCGCTCTGCCGGATCGCGCCAGTGAAGATGAAGGCTcggg                                                           >  1:1003582/1‑148 (MQ=255)
                                                                                              agagGTCGCTATACCGCCTTCCGCCGTTGATGTTATCGGCGTGCTGCCGCCCGTCGATAGCGTCACTGGCTACCAGGTAACCCCAGTGGTCGGCATTATCCCGCCCGATCTGCCGTATCGCGCCAGTGAAGATGAAGTCTCGGCGgtgt                                                      <  2:604855/149‑1 (MQ=255)
                                                                                               gagGTCGCTATACCGCCTTCCGCCGTTGAAGTTATCGGCGTGCTGCCGCCCGTGGATAGCGTCACTGGCTACCAGGTAACCCCAGTGGTCGGCATTATCCCGCCCGATCTGCCGTATCGCGCCAGTGAAGATGAAGTCTCGGCGGTGtt                                                     <  1:214257/149‑1 (MQ=255)
                                                                                                    cGCTATACCGCCTTCCGCCGTTGACGTTATCGGCGTGCTGCCGCCCGGCGATAGCGTCACTGGCTACCAGGTAACCCCAGTGGTCGGCATTATCCCGCCCGATCTGCCGTATCGCGCCAGTGAAGATGAAGTCTCGGCGGTGTTTGaaa                                                <  1:968399/149‑1 (MQ=255)
                                                                                                           ccGCCTTCCGCCGTTGAAGTTATCGGCGTGCTGCCGCCCGTCGATAGCGTCACTGGCTACCAGGTAACCCCAGTGGTCGGCATTATCCCGCCCGATCTGCCGTATCGCGCCAGTGAAGATGAAGTCTCGGCGGTGTTTGAAATGCCGCt                                         >  1:234197/1‑149 (MQ=255)
                                                                                                                 tCCGCCGTTGAAGTTATCGGCGTGCTGCCGCCCGTCGTTAGCGTCACTGGCTACCAGGTAACCCCAGTGGTCGGCATTATCCCGCCCGATCTGCCGTATCGCGCGAGTGATGATGAAGTCTCGGCGGTGTTTGAAATGCCGCTCGCCCa                                   <  1:250540/149‑1 (MQ=255)
                                                                                                                       gTTGAAGTTATCGGCGTGCTGCCGCCCGTCGATAGCGTCACTGGCTACCAGGTAACCCCAGTGGTCGGCATTATCCCGCCCGATCTGCCGTATCGCGCCAGTGAAGATGAAGTCTCGGCGGTGTTTGAAATGCCGCTCGCCCAGGCAtt                             <  2:918141/149‑1 (MQ=255)
                                                                                                                        ttGAAGTTATCGGCGTGCTGCCGCCCGTCGATAGCGTCACTGGCTACCCGGTACCCCCAGTGGTCGGCATTATCCCGCCCGATCTGCCGTATCGCGCCAGGGATGATGAAGTCTCGGCGGTGTTTGAACTGCCGCTCGCCCAGGCAtgc                            >  1:495963/1‑147 (MQ=255)
                                                                                                                         tGAAGTTATCGGCGTGCTGCCGCCCGTCGATAGCGTCACTGGCTACCAGGTAACCCCAGTGGTCGGCAGTATCCCGCCCGATCTGCCGTATCGCGCCAGTGAAGATGAAGTCTCGGCGGTGTTTGAAATGCCGCTCGCCCAGGCATTAc                           <  1:85220/149‑1 (MQ=255)
                                                                                                                              ctATCGACGGGCGGCAGCACGCCGATAGCGTCACTGGCTACCAGGTAACCCCAGTGGTCGGCATTATCCCGCCCGATCTGCCGTATCGCGCCAGTGAAGATGAAGTCTCGGCGGTGTTTGAAATGCCGCTCGCCCAGGCATTACATCTg                      >  2:503332/2‑149 (MQ=255)
                                                                                                                                aTCGGCGTGCTGCCGCCCGTCGATAGCGTCACTGGCTACCAGGTAACCCCAGTGGTCGGCATTATCCCGCCCGATCTGCCGTATCGCGCCAGTGAAGATGAAGTCTCGGCGGTGTTTGCAATGCCGCTCGCCCAGGCAGTACATCTggg                    >  1:940317/1‑149 (MQ=255)
                                                                                                                                aTCGACGGGCGGCAGCACGCCGATAGCGTCACTGGCTACCAGGAAACACCAGTGGTCGGCATTATCCCGCCCGATCTGCCGTATCGCCCCAGTGAAGATGAAGTCTCGGCGGGGTTTGAAATGCCGCTCGCCCAGGAATTACATGTggg                    >  1:183124/1‑149 (MQ=255)
                                                                                                                                    gCGTGCTGCCTCCCGTCGATAGCGTCACTGGCTACCAGGTAACCCCAGTGGTCGGCATTATCCCGCCCGATCTGCCGTATCGCGCCAGTGAAGATGAAGTCTCGGCGGTGTTTGTAATGCCGCTCGCCCAGGCATTACATCTGGGTCGt                <  2:586661/149‑1 (MQ=255)
                                                                                                                                    gCGTGCTGCCGCCCGTCGATAGCGTCACTGGCTACCAGGTAACCCCAGTGGTCGGCATTATCCCGCCCGATCTGCCGTATCGCGCCAGTGAAGCTGAAGTCTCGGCGGTGTTTGAAATGCCGCTCGCCCAGGCATTACATCTGGGTCGt                >  1:376848/1‑149 (MQ=255)
                                                                                                                                       tgctgcCGCCCGTCGATAGCGTCACTGGCTACCGGGTAACCCCAGTGGTCGGCATTATCCCGCCCGATCTGCCGTATCGCGCCAGTGAAGATGAAGTCTCGGCGGTGTTTGAAATGCCGCTCGCCCAGGCATTACATCTGGGTCGTTAt             <  1:803451/149‑1 (MQ=255)
                                                                                                                                            ccgccCGTCGATAGCGTCACTGGCTACCAGGTAACCCCAGTGGTCGGCATTATCCCGCCCGATCTGCCGTATCGCGCCAGTGAAGATGAAGTCTCGGCGGTGTTTGAAATGCCGCTCGCCCAGGCATTACATCTGGGTCGTTATCAccc        <  2:712227/149‑1 (MQ=255)
                                                                                                                                                  gTCGATAGCGTCACTGGCTACCAGGTAACCCCAGTGGTCGGCATTATCCCGCCCGATCTGCCGTATCGCGCCAGTGAAGATGAAGTCTCGGCGGTGTTTGAAATGCCGCTCGCCCAGGCATTACATCTGGGTCGTTATCACCCTTTAGa  <  1:395623/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
TGCGTAAACACGCTGGACAAGTGGCATTCCCTGGAGGTGCAGTCGATGACACGGACGCATCAGCTATCGCCGCCGCGCTGCGCGAAGCTGAAGAAGAGGTCGCTATACCGCCTTCCGCCGTTGAAGTTATCGGCGTGCTGCCGCCCGTCGATAGCGTCACTGGCTACCAGGTAACCCCAGTGGTCGGCATTATCCCGCCCGATCTGCCGTATCGCGCCAGTGAAGATGAAGTCTCGGCGGTGTTTGAAATGCCGCTCGCCCAGGCATTACATCTGGGTCGTTATCACCCTTTAGA  >  NC_000913/1896333‑1896627

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑