Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,019,460 G→A 100% V359I (GTC→ATC)  fliK → flagellar hook‑length control protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,019,4600GA100.0% 105.5 / ‑6.7 40V359I (GTC→ATC) fliKflagellar hook‑length control protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (17/21);  minor base C (2/0);  total (19/21)
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

AAGTGGCATTCAGTTAGGGCAAAGCAACATCAGTGGCGAAAGCTTTAGTGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCCGCGTCTTTTCCCCGCTTTGTTGCGCTCAAGACGCAGGATAATTAGCCGATAAGCAGTAGCGACACAGGA  >  NC_000913/2019315‑2019605
                                                                                                                                                 |                                                                                                                                                 
aaGTGGCATTCAGTTAGGGCAAAGCAACATCATTGGCGAAAGCTTTAGTGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCAtct                                                                                                                                                <  1:341549/149‑1 (MQ=255)
      cATTCAGTTAGGGCAAAGCAACATCAGTGGCGACAGCTTTAGTGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTAc                                                                                                                                          >  2:925659/1‑149 (MQ=255)
       aTTCAGTTAGGGCAAAGCAACATCAGTGGCGAAAGCTTTAGTGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACa                                                                                                                                         >  2:423535/1‑149 (MQ=255)
            gTTAGGGCAAAGCAACATCAGTGGCGAAAGCTTTAGGGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGc                                                                                                                                    <  2:883120/149‑1 (MQ=255)
               aGGGCAAAGCAACATCAGTGGCGAAAGCTTTAGTGGTCAGCAGCAGGCCGCTTCCCAGCATCAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCgtg                                                                                                                                 <  1:879114/149‑1 (MQ=255)
                   cAAAGCAACATCAGTGGCGAAAGCTTTAGTGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCACAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCATCCGGTTCCCAGCTCTTTACAAGGGCGTGTAAc                                                                                                                             >  1:303403/1‑149 (MQ=255)
                    aaaGCAACATCAGTGGCGAAAGCTTTAGTGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGTGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACa                                                                                                                            <  1:148658/149‑1 (MQ=255)
                      aGCAACATCAGTGGCGAAAGCTTTAGTGGTCAGCAACAGGCCGCTTCCCAGAAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCAGCTCTTTACAAGGGCGGGTAACAgg                                                                                                                          <  1:644319/149‑1 (MQ=255)
                       gCAACATCAGTGGCGACAGCTTTAGTGGGCAGCAGCAGGGAGATTCCCAGCAACAGCAAAGCCAACGGACAGCAAACCATGAACCTCTGGGGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGc                                                                                                                         <  2:855295/149‑1 (MQ=255)
                                gTGGCGAAAGCTTTAGTGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTGCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAgcggc                                                                                                                >  1:53447/1‑149 (MQ=255)
                                     gAAAGCTTTAGTGGTCAGCAGCAGGCCGCTTCCCTGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGa                                                                                                           <  1:217028/149‑1 (MQ=255)
                                     gAAAGCTTTAGTGGTCAGCAGCAGGCCGCTTCCCAGCACCAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGCCGCCGATACGCTTCCGGTTCCCAGCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGa                                                                                                           >  2:623535/1‑149 (MQ=255)
                                       aaGCTTTAGTGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGata                                                                                                         <  1:392524/149‑1 (MQ=255)
                                          cTTTAGTGGGCAGCCGCTTGCCTCTTCCCAGCAACATCTAAGCCATCGCACAGCAACCCATGATCCTCTGGCGGTGGAAGACGACGATACGATTCAGGTTCCCATCTCTTTACAAGGTCGTTTAACAGGAAACAGCGGCGTTGATAttt                                                                                                      <  2:473566/149‑1 (MQ=255)
                                           tttAGTGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCATAGCCTACGCAGAGAAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGTTAtttt                                                                                                     <  1:514018/149‑1 (MQ=255)
                                            ttAGTGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAAACATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTc                                                                                                    <  1:715155/149‑1 (MQ=255)
                                              aGTCGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCCTGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGc                                                                                                  <  1:1015568/149‑1 (MQ=255)
                                                  gTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTaa                                                                                              >  1:547291/1‑149 (MQ=255)
                                                   tCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAAc                                                                                             <  2:362198/149‑1 (MQ=255)
                                                           agGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAATCATGCACCTCTGGCGGGGGAAGCCGACGATACGCTTCCGGTTCCCCTCTCTTTACATGGCCGTGTAACAGGCAACAGCGGCGTAGCTATATTCGGCGACCGTCAGAgg                                                                                     >  1:662735/1‑149 (MQ=255)
                                                             gCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTAGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACCGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGtt                                                                                   >  1:350429/1‑148 (MQ=255)
                                                              ccGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAg                                                                                  >  1:956677/1‑149 (MQ=255)
                                                                              gcaaAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCCGCGTc                                                                  >  1:1016585/1‑149 (MQ=255)
                                                                              gcaaAGCCAACCCACAGCAAACCATGAACCTCTGGCGGGGGATGACGACGATACGCGTCCGGTTCCCCTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGCGGTAGCACCGTAATCCGCGTc                                                                  >  1:734985/1‑149 (MQ=255)
                                                                                  aGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCCGCGTCtttt                                                              <  2:53447/149‑1 (MQ=255)
                                                                                         gCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTACCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTACGCCAAACGTCAGAGGTAGCACCGTAATCCGCGTCTTTTCCCCGCt                                                       <  1:269363/149‑1 (MQ=255)
                                                                                              gCAAACCATGAACCTCTGGCTGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCCGCGTCTTTTCCCCGCTttgtt                                                  <  1:254920/149‑1 (MQ=255)
                                                                                               cAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTACCGTCAGAGGTAGAACCGTAATCAGCGTATTTTCCCCGCTttgttg                                                 >  2:117914/1‑149 (MQ=255)
                                                                                                  aCCATGAACCTCTGGCGGGGGAAGCCGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGGGGAACAGGCAACAGCGGCGGTGTTATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCCGCGTCTTTTCCCCTCTTTGATgcgc                                              >  1:603679/1‑149 (MQ=255)
                                                                                                  aCCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCCGCGTCTTTTCCCCGCTTTGTTgcgc                                              >  1:812670/1‑149 (MQ=255)
                                                                                                             ctGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCCGCGTCTTTTCCCCGCTTTGTTGCGCTCAAGACGCAg                                   <  1:423535/149‑1 (MQ=255)
                                                                                                                  gggggACGACGACGATACGCTTCCGGTTCCCATCTCTATACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTATCGCCTAACGTCAGAGGTAGCACCGTAATCCGCGTCTTTTCCCCGCTTTGTTGCGCTCAAGACGCAGGATaa                              <  1:320998/149‑1 (MQ=255)
                                                                                                                   ggggTAGCCGACGATACGCTTCCGGTTCGCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCCGCGTCTTTTCCCCGCTTTGTTGCGCTCAAGACGCAGGATAAt                             <  2:171850/149‑1 (MQ=255)
                                                                                                                       aaGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCCGCGTCTTTTCCCCGCTTTGTTGCGCTCAAGACGCAGGATAATTAGc                         <  2:812670/149‑1 (MQ=255)
                                                                                                                         gacgacGATACGCTTCCGGGTCCCATCTCTTTACAAGGGCGAGTAACAGGCAACAGCGGCGGTGATAATTTCGCCTAACGTCAGAGGTAGCACCGTAATCCGCGTCTTTTCGACGATTTGTTGAGCTCAAGACGCAGTATAATTAGcag                       >  1:492564/1‑147 (MQ=255)
                                                                                                                           cgacgaTACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCCGCGTCTTTTCCCCGCTTTGTTGCGCTCAAGACGCAGGATAATTAGCCGAt                     <  1:321661/149‑1 (MQ=255)
                                                                                                                            gacgaTACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACATGCAACAGCGGCGTTGATATTTTCGCCTAACGACAGAGGTAGCACCGAAATCCGCGTCTTTTCCCCGCTTTGTTGCGCTCAAGACGCAGGAGAATTAGCCGATa                    >  1:581510/1‑149 (MQ=255)
                                                                                                                                    gCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCAGCGTCTTTTCCCCGCTTTGTTGCGCTCAAGACGCAGGATAATTAGCCGATAAGCAGTAg            >  1:897709/1‑149 (MQ=255)
                                                                                                                                          ggTTCCCATCTC‑TTACAAGGGCGTGTAACAGGCAACAGCGGCGATGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCCGCGTCTTTTCCCCGCTTTGTTGAGCTCAAGACGCAGGATAATTATGCGATAAGCAGTAGCGAcaca     >  1:362402/1‑149 (MQ=255)
                                                                                                                                              cccATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCCGCGTCTATTCCCCGCTTTGTTGCGCTCAAGACGCAGGATAATTAGACGATAATCAGTAGCGACACAGGa  >  1:184183/1‑149 (MQ=255)
                                                                                                                                                 |                                                                                                                                                 
AAGTGGCATTCAGTTAGGGCAAAGCAACATCAGTGGCGAAAGCTTTAGTGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCCGCGTCTTTTCCCCGCTTTGTTGCGCTCAAGACGCAGGATAATTAGCCGATAAGCAGTAGCGACACAGGA  >  NC_000913/2019315‑2019605

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑