Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,022,335 C→T 9.0% T156I (ACC→ATC)  fliP → flagellar biosynthesis protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,022,3350CT 9.0% 119.9 / 3.1 46T156I (ACC→ATC) fliPflagellar biosynthesis protein
Reads supporting (aligned to +/- strand):  ref base C (21/20);  new base T (2/2);  total (23/23)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.83e-01

AATTTATGTAGATGCGTACCAGCCATTCAGCGAAGAGAAAATATCAATGCAGGAGGCGCTGGAAAAAGGGGCGCAGCCGCTGCGTGAGTTTATGCTGCGTCAGACCCGTGAGGCAGATTTAGGGTTGTTTGCCAGACTGGCGAATACCGGCCCGTTGCAGGGACCTGAAGCCGTGCCGATGCGCATTTTGCTCCCGGCCTACGTGACCAGCGAGTTGAAAACCGCATTTCAGATAGGCTTCACGATTTTCATCCCTTTTTTGATTATCGACCTGGTGATAGCCAGCGTGTTGAT  >  NC_000913/2022189‑2022482
                                                                                                                                                  |                                                                                                                                                   
aaTTTATGTAGATGCGTACCAGCCATTCAGCGAAGAGAAAATATCAATGCAGGAGGCGCTGGAAAAAGGGGCGCAGCCGCTGCGTGAGTTTATGCTGCGTCAGACCCGTGAGGCAGCTTTAGGGTTGTTTGCCAGACTGGCGAATACCg                                                                                                                                                   >  1:746631/1‑149 (MQ=255)
   ttATGTAGATGCGTACCAGCCATTCAGCGAAGAGAAAATATCAATGCAGGAGGCGCTGGAAAAAGGGGCGCCGCCGCTGCGTGAGTTTATGCTGCGTCAGACCCGTGAGGCAGATTTAGGGTTGTTTGCCAGACTGGCGAATACCGGcc                                                                                                                                                >  1:366472/1‑149 (MQ=255)
         aGATGCGTACCAGCCATTCAGCGAAGAGAAAATATCAATGCAGGAGGCGCTGGAAAAAGGGGCGCAGCCGCTGCGTGAGTTTATGCTGCGGCAGACCCGTGAGGCAGATTTAGGGTTGTTTGCCAGACTGGCGAATACCGGCCCGTTGc                                                                                                                                          <  1:502792/149‑1 (MQ=255)
          gATGCGTACCAGCCATTCAGCGAAGAGAAAATATCAATGCAGGAGGCGCTGGAAAAAGGGGCGCAGCCGCTGCGTGAGTTTATGCTGCGTCAGACCCGTGAGGCAGATTTAGGGTTGTTTGCCAGACTGGCGAATATCGGCCCGTTGCa                                                                                                                                         >  2:512116/1‑149 (MQ=255)
          gATGCGTACCAGCCATTCAGCGAAGAGAAAATATCAATGCAGGAGGCGCTGGAAAAAGGGGCGCAGCCGCTGCGTGAGTTTATGCTGCGTCAGACCCGTGAGGCAGATTTAGGGTTGTTTGCCAGACTGGCGAATATCGGCCCGTTGCa                                                                                                                                         >  2:507865/1‑149 (MQ=255)
            tGCGTACCAGCCATTCAGCGAAGAGAAAATATAAATGCAGGAGGCGCTGGAAAAAGGGGCGCAGCCGCTGCGTGAGTTTATGCTGCGTCAGACCCGTGAGGCAGATTTAGGGTTGTTTGCCAGACTGGCGAATACCGGCCCGTTGCAgg                                                                                                                                       >  1:910580/1‑149 (MQ=255)
               gttccAGCCATTCATCGTAGACAAAATCTCACTCCAGGAGGCGCGGGAAAAAGGGGCCCAGCCGCTGCGTGAGTTTATGCTGCGTCAGTCCCGTGAGGCAGATTTAGGGTTGTTTGCCAGACTGGCGAATACCGGCCCGTTGCAGGGAc                                                                                                                                    <  1:153768/146‑1 (MQ=255)
                        aTTCCGCGAAGAGAAAATATCAATGCAGGAGGCGCTGGAAAAAGGGGCGCAGCCGCTGCGTGAGTTTATGCTGCGTCAGACCCGTGAGGCACATTTAGGGTTGTTTGCCAGACTGGCGAATACCGGCCCGTTGGAGGGACCTGAAGCCg                                                                                                                           >  2:781980/1‑149 (MQ=255)
                               gAAGAGAAAATATCAATGCAGGAGGCGCTGGAAAAAGGGGCGCAGCCGCTGCGTGAGTTTATGCTGCGTCAGACCCGTGAGGCAGATTTAGGGTTGTTTGCCAGACTGGCGAATACCGGCCCGTTGCAGGGACCTGAAGCCGTGCCGAt                                                                                                                    >  1:169891/1‑149 (MQ=255)
                                     aaaaTATCAATGCAGGAGGCGCTGGAAAAAGGGGCGCAGCCGCTGCGTGAGTTTATGCTGCGTCAGACCCGTGAGGCAGATTTAGGGTTGTTTGCCAGACTGGCGAATACCGGCGCGTTGCAGGGACCTGAAGCCGTGCCGATGCGCAt                                                                                                              >  2:544291/1‑149 (MQ=255)
                                       actagcAATGCCGGAGGCGCGGGAAACAGGGGCGCAGCCGCTGCGTGAGTTTATGCTGCGGCAGACCCGTGAGGCAGATTTAGGGGTGTTTGCCAGACTGGCGAATACCGGCCCGTTGCAGGGACCTGAAGCCGTGCCGATGCGCAttt                                                                                                            <  1:874792/144‑1 (MQ=255)
                                       aaTATCAATGCAGGAGGCGCTGGAAAAAGGGGCGCAGCCGCTGCGTGAGTTTATGCTGCGTCAGACCCGTGAGGCAGATTTAGGGTTGTTTGCCAGACTGGCGAATACCGGCCCGTTGCAGGGACCTGAAGCCGTGCCGATGCGCAttt                                                                                                            >  1:30636/1‑149 (MQ=255)
                                            cacttcAGGAGGCGCTGGAAAAAGGGGCGCATCCGCTGCGTGAGGTTATGCTGCGTCAGACCCGTGAGGCAGATTTAGGGGTGTTTGCCAGACTGGCGAATACCGGCCCGTTGGAGGGACCTGAAGCCGTGCCGATGCGCATTTTGCTc                                                                                                       <  2:963812/144‑1 (MQ=255)
                                              aTGCAGGAGGCGCTGGAAAACGGGGCGCAGCCGCTGCGTGAGTTTATGCTGCGTCAGACCCGTGAGGCAGATTTAGGGTTGTTTGCCAGACTGGCGAATACCGGCCCGTTGCAGGGACCTGAAGCCGTGCCGATGCGCATTTTGCTccc                                                                                                     <  1:257833/149‑1 (MQ=255)
                                                              aaaaaGGGGCGCAGCCGCTGCGTGAGTTTATGCTGCGTCAGACCCGTGAGGCAGATTTAGGGTTGTTTGCCAGACTGGCGAATACCGGCCCGTTGCAGGGACCTGAAGCCGTGCCGATGCGCATTTTGCTCCCGGCCTACGTGACCAGc                                                                                     <  2:910580/149‑1 (MQ=255)
                                                                  acgcgcgcAGCCGCTGCGTGAGTTTATGCTGCGTCAGACCCGTGAGGCAGATTTAGGGTTGTTTGCCAGACTGGCGAATACCGGCCCGTTGCAGGGACCTGAAGCCGTGCCGATGCGCATTTTGCTCCCGGCCTACGTGACCAGCGAGt                                                                                 <  2:545486/145‑1 (MQ=255)
                                                                  accggCGCAGCCGCTGCGTGAGTTTATGCGGCGTCAGACCCGTGAGGCAGATTTAGGGTTGTTTGCCAGACTGGCGAATACCGGCCCGTTGCAGGGACCTGAAGCCGTGCCGATGCGCATTTTGCTCCCGGCCTACGTGACCAGCGAGt                                                                                 <  2:549915/146‑1 (MQ=255)
                                                                  aGGGGCGCAGCCGCTGCGTGAGTTTATGCTGCGTCAGACCCGTGAGGCAGATTTAGGGTTGTTTGCCAGACTGGCGAATACCGGCCCGTTGCAGGGACCTGAAGCCGTGCCGATGCGCATTTAGCTCCCGGCCTACGTGACCAGCGAGt                                                                                 >  2:305485/1‑149 (MQ=255)
                                                                              gCTGCGTGAGTTTATGCTGCGTCAGACCCGTGAGGCAGATTTAGGGTTGTTTGCCAGACTGGCGAATACCGGCCCGTTGCAGGGACCTGAAGCCGTGCCGATGCGCATTTTGCTCCCGGCCTACGTGACCAGCGAGTTGAAAACCGCAt                                                                     >  2:287259/1‑149 (MQ=255)
                                                                               cTGCGTGAGTTTATGCTGCGTCAGACCCGTGAGGCAGATTTAGGGTTGTTTGCCAGACTGGCGAATACCGGCCCGTTGCAGGGACCTGAAGCCGTGCCGATGCGCATTTTGCTCCCGGCCTACGTGACCAGCGAGTTGAATACCGCAtt                                                                    >  2:292923/1‑149 (MQ=255)
                                                                                  cGTGAGTTTATGCTGCGTCAGACCCGTGAGGCAGATTTAGGGTTGTTTGCCAGACTGGCGAATACCGGCCCGTTGCAGGGACCTGAAGCCGTGCCGATGCGCATTTTGCTCCCGGCCTACGTGACCAGCGAGTTGAAAACCGCATTTct                                                                 >  1:901109/1‑148 (MQ=255)
                                                                                  cGTGAGTTTATGCTGCGTCAGACCCGTGAGGCAGATTTAGGGTTGTTTGCCAGACTGGCGAATACCGGCCCGTTGCAGGGACCTGAAGCCGTGCCGATGCGCATTTTGCTCCCGGCCTACGTGACCAGCGAGTTGAAAACCGCATTTCa                                                                 >  1:875834/1‑149 (MQ=255)
                                                                                   gTGAGTTTATGCTGCGTCAGACCCGTGAGGCAGATTTAGGGTTGTTTGCCAGACTGGCGAATACCGGCCCGTTGCAGGGACCTGAAGCCGTGCCGATGCGCATTTTGCTCCCGGCCTACGTGACCAGCGAGTTGAAAACCGCATTTCAg                                                                >  1:641739/1‑149 (MQ=255)
                                                                                         ttATGCTGCGTCAGACCCGTGAGGCAGATTTAGGGTTGTTTGCCAGACTGGCGAATACCGGCCCGTTGCAGGGACCTGAAGCCGTGCCGATGCGCATTTTGCTCCCGGCCTACGTGACCAGCGAGTTGAAAACCGCATTTCAGATAGGc                                                          <  2:169891/149‑1 (MQ=255)
                                                                                           aTGCTGCGGCAGACCCGTGAGGCAGATTTAGGGATGTTTGCCAGACAGGCGAATAGCGGCGCGTTGCAGGGACCTGAAGCCGTGCCGATGCGCATTTTGCTCCCGGCCTACGTGACCAGCGAGTTGAAAACCGCATTTCAGATAGGCtt                                                        <  1:727813/149‑1 (MQ=255)
                                                                                               tgcGTCAGACCCGTGAGGCAGATTTAGGGTTGTTTGCCAGACTGGCGAATACCGGCCCGTTGCAGGGACCTGAAGCCGTGCCAATGCGCATTTTGCTCCCGGCCTACGTGTCCAGCGAGTTGAAACCCGCATTTCAGATAGGCTTCACg                                                    >  1:864385/1‑149 (MQ=255)
                                                                                                   tCAGACCCGTGAGGCAGATTTAGGGTTGTTTGCCAGACTGGCGAATACCGGCCCGTTGCAGGGACCTGAAGCCGTGCCGATGCGCATTTTGCTCCCGGCCTACGTGACCAGCGAGTTGAAAACCGCATTTCAGATAGGCTTCACGAttt                                                >  2:440473/1‑149 (MQ=255)
                                                                                                      gACCCGTGAGGCAGATTTAGGGTTGTTTGCCAGACTGGCGAATACCGGCCCGTTGCAGGGACCTGAAGCCGTGCCGATGCGCATTTTGCTCCCGGCCTACGTGACCAGCGAGATGAAAACCGCAGTTCAGATAGTCTTCACGATTTTCa                                             >  1:674517/1‑149 (MQ=255)
                                                                                                       aCCCGTGAGGCAGATTTAGGGTTGTTTGCCAGACTGGCGAAAACCGGCCCGTAGCAGGGACCTGAAGCCGTGCCGATGCGCATTTTGCTCCCGGCCTACGTGACCAGCGAGTTGAAAACCGCATTTCAGATAGGCTTCACGATTTTCAt                                            >  2:285527/1‑149 (MQ=255)
                                                                                                          cGTGAGGCAGATTTAGGGTTGTTTGCCAGACTGGCGAATACCGGCCCGTTGCAGGGACCTGAAGCCGTGCCGATGCGCATTTTGCTCCCGGCCTACGTGACCAGCGAGTTGAAAACCGCATTTCAGATAGGCTTCACGATTTTCATccc                                         >  1:524436/1‑149 (MQ=255)
                                                                                                                gCAGATTTAGGGTTGTTTGCCAGACTGGCGAATATCGGCCCGTTGCAGGGACCTGATGCCGTGCCGATGCGCATTTTGCTCCCGGCCTACGTGACCAGCGAGTTGAAAACCGCATTTCAGATAGGCTTCACGATTTTCATCCCtttttt                                   <  1:507865/149‑1 (MQ=255)
                                                                                                                gCAGATTTAGGGTTGTTTGCCAGACTGGCGAATATCGGCCCGTTGCAGGGACCTGAAGCCGGGCCGATGCGCATTTTGCTCCCGGCCTACGTGACCAGCGAGTTGAAAACCGCATTTCAGATAGGCTTCACGATTTTCATCCCtttttt                                   <  1:512116/149‑1 (MQ=255)
                                                                                                                gCAGATTTAGGGTTGTTTGCCAGACTGGCGAATACCGGCCCGTTGCAGGGACCTGAAGCCGTGCCGATGCGCATTTTGCTCCCGGCCTACGTGACCAGCGAGTTGAAAACCGCATTTCAGATAGGGTTCACGATTTTCATCCCtttttt                                   >  2:85932/1‑149 (MQ=255)
                                                                                                                 cAGAATTAGGGTTGTTTGCCAGACAGGCGAATACCGGCCCGTTGCAGGGACCTTAAGCCGTGCCGAAGCGCATTTTGCTCCCGGCCTACGTGACCAGCGAGTTGAAAACCGCATTTCAGATATGCTTCACGATTTTCATACATTTTTTg                                  <  2:888243/149‑1 (MQ=255)
                                                                                                                  aGATTTAGGGTTGTTTGCCAGACTGGCGAATACCGGCCCGTTGCAGGGACCTGAAGCCGTGCCGATGCGCATTTTGCTCCCGGCCTACGTGACCAGCGAGTTGAAAACCGCATTTCAGATAGGCTTCACGATTTTCATCCCTTTTTTGa                                 <  2:641739/149‑1 (MQ=255)
                                                                                                                    aTTTAGGTTTGTTTGCCAGACTGGCGAATACCGGCCCGTTGCAGGGACCTGAAGCCGTGCCGATGCGCATTTTGCTCCCGGCCTTCGTGACCAGCGAGTTGAAAACCGCATTTCCGAGAGGCTTCACGATATTCATCCCTTTTTTGAtt                               <  1:431954/149‑1 (MQ=255)
                                                                                                                    aTTTAGGGGGGTTTGCCAGACTGGCGAATACCGGCCCGTTGCAGGGACCTGAAGCCGTGCCGATGCGCATTTTGCTCCCGGCCTACGTGACCAGCGAGTTGAAAACCGCATTTCAGATAGGCTTCACGATTTTCATCCCTTTTTTGAtt                               <  1:245361/149‑1 (MQ=255)
                                                                                                                            ttgttTGCCAGACTGGCGAATACCGGCCCGTTGCATGGACCTGAAGCCGTGCCGATGCGCATTTTGCTCCCGGCCTACGTGACCAGCGAGATGAAAACCGCATTTAAGATAGTCTGCACGATTTTCATCCATTTTTTGATTaaagaact                       >  2:572166/1‑142 (MQ=255)
                                                                                                                                   ccAGACTGGCGAATACCGGCCCGTTGCAGGGACCTGAAGCCGTGCCGATGCGCATTTTGCTCCCGGCCTACGTGACCAGCGAGTTGAAAACCGCATTTCAGATAGGCTTCACGATTTTCATCCCTTTTTTGATTATCGACCTGGTGATa                <  1:95884/149‑1 (MQ=255)
                                                                                                                                    cAGACTGGCGAATACCGGCCCGTTGCAGGGACCTGAAGCCGTGCCGATGCGCATTTTGCTCCCGGCCTACGTGACCAGCGAGTTGAAAACCGCATTTCAGATAGGCTTCACGATTTTCATCCCTTTTTTGATTATCGACCTGGTGATAg               <  1:516842/149‑1 (MQ=255)
                                                                                                                                         ttgCGAATACCGGCCCGTGGCAGGGACCTGAAGCCGTGCCGATGCGCATTTTGCTCCCGGCCTACGTGACCAGCGAGTTGAAAACCGCATTTCAGATAGGCTTCACGATTTTCATCCCTTTTTTGATTATCGAGCTGGTGATAGCCAGc          <  2:476888/147‑1 (MQ=255)
                                                                                                                                         tGGCGAATACCGGCCCGTTGCAGGGACCTGAAGCCGTGCCGATGCGCATTTTGCTCCCGGCCTACGTGACCAGCGAGTTGAAAACCGCATTTCAGATAGGCTTCACGATTTTCATCCCTTTTTTGATTATCGACCTGGTGATAGCCAGc          <  1:350961/149‑1 (MQ=255)
                                                                                                                                         gggCGAATACCGGCCCGTTGCAGGGACCTGAAGCCGTGCCGATGCGCATTTTGCTCCCGGCCTACGTGACCAGCGAGTTGAAAACCGCATTTCAGATAGGCTTCACGATTTTCATCCCTTTTTTGATTATCGACCTGGGGATAGCCAGc          <  1:551561/148‑1 (MQ=255)
                                                                                                                                           gCGAATACCGGCCCGTTGCAGGGACCTGAAGCCGTGCCGATGCGCATTTTGCTCCCGGCCTACGTGACCAGCGAGTTGAAAACCGCATTTCAGATAGGCTTCACGATTTTCATCCCTTTTTTGATTATCGACCTGGTGATAGCCAGCgt        >  1:607747/1‑149 (MQ=255)
                                                                                                                                                 aCCGGCCCGTTGCAGGGACCTGCAGCCGGGCCGATGCGCATTTTGCTCCCGGCCTTCGTGACCAGCGAGTTGAAATCCGCATTTCAGATAGGCGTCACGATTTTCATCCCTTTTTTGATTATCGACCTGGTGATAGCCAGCGTGTTGAt  <  1:434560/149‑1 (MQ=255)
                                                                                                                                                 aCCGGCCCGTTGCAGGGACCTGAAGCCGTGCCGATGCGCATTTTGCTCCCGGCCTACGTGACCAGCGAGTTGAAAACCGCATTTCAGATAGGCTTCACGATTTTCATCCCTTTTTTGATTATCGACCTGGTGATAGCCAGCGTGTTGAt  <  1:509897/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                   
AATTTATGTAGATGCGTACCAGCCATTCAGCGAAGAGAAAATATCAATGCAGGAGGCGCTGGAAAAAGGGGCGCAGCCGCTGCGTGAGTTTATGCTGCGTCAGACCCGTGAGGCAGATTTAGGGTTGTTTGCCAGACTGGCGAATACCGGCCCGTTGCAGGGACCTGAAGCCGTGCCGATGCGCATTTTGCTCCCGGCCTACGTGACCAGCGAGTTGAAAACCGCATTTCAGATAGGCTTCACGATTTTCATCCCTTTTTTGATTATCGACCTGGTGATAGCCAGCGTGTTGAT  >  NC_000913/2022189‑2022482

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑