Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,715,721 T→A 9.5% L100H (CTT→CAT)  eamB → cysteine and O‑acetylserine exporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,715,7210TA 9.5% 124.2 / 3.0 56L100H (CTT→CAT) eamBcysteine and O‑acetylserine exporter
Reads supporting (aligned to +/- strand):  ref base T (29/21);  new base A (4/2);  total (33/23)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TGATGTTACTGTGTGCGGGCATTTCATTTTCACTGGCAGTGATTGACCCGGCAGCGGTACACCTTTTGAGTTGGGCGGGGGCGGCATATATTGTCTGGCTGGCGTGGAAAATCGCCACCAGCCCAACAAAGGAAGACGGACTTCAGGCAAAACCAATCAGCTTTTGGGCCAGCTTTGCTTTGCAGTTTGTGAACGTCAAAATCATTTTGTACGGTGTTACGGCACTGTCGACGTTTGTTCTGCCGCAAACACAGGCGTTAAGCTGGGTAGTTGGCGTCAGCGTTTTG  >  NC_000913/2715580‑2715866
                                                                                                                                             |                                                                                                                                                 
tgATGTTACTGTGTGCGGGCATTTCATTTTCACTGGCAGTGATTGACCCGGCAGCGGTACACCTTTTGAGTTGGTCGGGGGCGGCATATATTGTCTGGCTGGCGTGGAAAATCGCCACCAGCCAAACAAAGGAAGACGGACTTAAGGCa                                                                                                                                            >  1:215998/1‑149 (MQ=255)
tgATGTTACTGTGTGCGGGCATTTCATTTTCACTGGCAGTGATTGACCCGGCAGCGGTACACCTTTTGAGTTGGGCGGGGGCGGCATATATTGTCTGGCTGGCGTGGAAAATCGCCACCAGCCCAACAAAGGAAGACGGACTTCAGGCa                                                                                                                                            >  1:154259/1‑149 (MQ=255)
tgATGTTACTGTGTGCGGGCATTTCATTTTCACTGGCAGTGATTGACCCGGCAGCGGTACACCTTTTGAGTTGGGCGGGGGCGGCATATATTGTCTGGCTGGCGTGGAAAATCGCCACCAGCCCAACAAAGGAAGACGGACTTCAGGCa                                                                                                                                            >  1:786962/1‑149 (MQ=255)
                 gtcatttaatttTCCCTGGCAGTTATTGACCCGGCAGCGTTTCACCTTTTGAGTTGGGCTGTGGCGGCATATATTGTCTGGCTGGCGTGGAAAATAGCCACCAGACCAACAAAGGAAGACGGACTTCAGGCAAAACCAATCAGCTTTTg                                                                                                                           <  1:720733/147‑1 (MQ=255)
                    atttcatttTCACTGGCAGTGATTGACCCGGCAGCGGTACACCTTTTGAGTTGGGCGGGGGCGGCATATATTGTCTGGCTGGCGAGGAAAATCGCCACCAGCCCAACAAAGGAAGACGGACATCAGGCAAAACCAATCAGCTGTTGGGc                                                                                                                        >  2:516205/1‑149 (MQ=255)
                      ttcatttTCACTGGCAGTGATTGACCCGGCAGCGGTACACCTTTTGAGTTGGGCGGGGGCGGCATATATTGTCTGGCTGGCGTGGAAAATCGCCACCAGCCCAACAAAGGAAGACGGACTTCAGGCAAAACCAATCAGCTTTTGGTCCa                                                                                                                      >  2:628619/1‑149 (MQ=255)
                      ttcatttTCACTGGCAGTGATTGACCCGGCAGCGGTACACCTTTTGAGTTGGGCGGGGGCGGCATATATTGTCTGGCTGGCGTGGAAAATCGCCACCAGCCCAACAAAGGAAGACGGACTTCAGGCAAAACCAATCAGCTTTTGGGCCa                                                                                                                      >  2:625141/1‑149 (MQ=255)
                       tcatttTCACTGGCAGTGATTGACCCGGCAGCGGTACACCTTTTGAGTTGGGCGGGGGCGGCATATATTGTCTGGCTGGCGTGGAAAATCGCCACCAGCCCAACAAAGGAAGACGGACTTCAGGCCAAACCAATCAGCTGTTGGGCCAg                                                                                                                     >  1:1007478/1‑149 (MQ=255)
                         atttTCACTGGCAGTGATTGACCCGGCCGCGGTACACCTTTTGAGTTGGGCGGGGGCGGCATATATTGTCTGGCTGGCGTGGAAAATCGCCCCCAGCCCAACAAAGGAAGACGGACTTCAGGCAAAACCAATCAGCTTTTGGGCCAgct                                                                                                                   >  1:8642/1‑149 (MQ=255)
                         atttTCACTGGCAGTGATTGACCCGGCAGCGGTACACCTTTTGAGTTGGGCGGGGGCGGCATATATTGTCTGGCTGGCGTGGAAAATCGCCACCAGCCCAACAAAGGAAGACGGACTTCAGGCAAAACCAATCAGCTTTTGGGCCAgct                                                                                                                   >  2:945172/1‑149 (MQ=255)
                         atttTCACTGGCAGTGATTGACCCGGCAGCGGTACACCTTTTGAGTTGGGCGGGGGCGGCATATATTGTCTGGCTGGCGTGGAAAATCGCCACCAGCCCAACAAAGGAAGACGGACTTCAGGCAAAACCAATCAGCTTTTGGGCCAgct                                                                                                                   >  1:436501/1‑149 (MQ=255)
                           tttCACTGGCAGTGATTGACCCGGCAGCGGTACACCTTTTGAGTTGGGCGGGGGCGGCATATATTGTCTGGCTGGCGTGGAAAATCGCCACCAGCCCAACAAAGGAAGACGGACTTCAGGCAAAACCAATCAGCTTTTGGGCCAgcttt                                                                                                                 >  2:512042/1‑149 (MQ=255)
                           tatCACTGGGAGTCATTGACCCGGCAGCGGTACACCTTTTGAGTTGGGCGGGGGCGGCATATCTTGTCTGGGTTGCGTGGAAAATCGCCACCAGCCCAACATAGGAAGACGGACTTCAGGCAAAACCAATCAGCTTTTGGGCCAgcttt                                                                                                                 <  2:310511/147‑1 (MQ=255)
                            ttCACTGGCAGTGATTGACCCGGCAGCGGTACACCTTTTGAGTTGGGCGGGGGCGGCATATATTGTCTGGCTGGCGTGGAAAATCGCCACCAGCCCAACAAAGGAAGACGGACTTCAGGCAAAACCAATCAGCTTTTGGGCCAgctttg                                                                                                                <  1:312370/149‑1 (MQ=255)
                                     aGTGATTGACCCGGCAGCGGTACACCTTTTGAGTTGGGCGGGGGCGGCATATATTGTCTGGCTGGCGTGGAAAATCGCCACCAGCCCAACAAAGGAAGACGGACTTCAGGCAAAACCAATCAGCTTATGGGCCAGCTTTGCTTTGCAGt                                                                                                       >  1:90913/1‑149 (MQ=255)
                                         aTTGACCCGGCAGCGGTACACCTTATGAGTTGGGCGGGGGCGGCATATTTTGTCTGGCTGGCGTGGAAAATCGCCACCAGCCCAAAAATGGAAGACGGACTTCAGGCAAAACCAATCAGCTTTTGGGCCAGCTTTGCTTTGCAGTTtgg                                                                                                   <  2:51725/149‑2 (MQ=255)
                                            gACCCGGCAGCGGTACACCTTTTGAGTTGGGCGGGGGCGGCATATATTGTCTGGCTGGCGTGGAAAATCGCCACCAGCCCAACAAAGGAAGACGGACTTCAGGCAAAACCAATCAGCTTTTGGGCCAGCTTTGCTTTGCAGTTTGTGaa                                                                                                >  2:270568/1‑149 (MQ=255)
                                               ccGGCAGCGGTACACCTTTTGAGTTGGGCGGGGGCGGCATATATTGTCTGGCTGGCGTGGAAAATCGCCACCAGCCCAACAAAGGAAGCCGGCCTTCTGGCAAAACCAATCAGCTTTTGGGCCAGCTTTGCTGTGCAGTTTGTGAACGt                                                                                             >  1:180461/1‑149 (MQ=255)
                                               ccGGCAGCGGTACACCTTTTGAGTTGGGCGGGGGCGGCATATATTGTCTGGCTGGCGTGGAAAATCGCCACCAGCCCAACAAAGGAAGACGGACTTCAGGCAAAACCAATCAGCTTTTGGGCCAGCTTTGCTTTGCAGttt                                                                                                     <  1:720268/141‑1 (MQ=255)
                                               ccGGCAGCGGTACACCTTTTGAGTTGGGCGGGGGCGGCATATATTGTCTGGCTGGCGTGGAAAATCGCCACCAGCCCAACAAAGGAAGACGGACTTCAGGCAAAACCAATCAGCTTTTGGGCCAGCTTTGCTTTGCAGttt                                                                                                     >  2:720268/1‑141 (MQ=255)
                                                      cGGTACACCTTTTGAGTTGGGCGGGGGCGGCATATATTGTCTGGCTGGCGTGGAAAATCGCCACCAGCCCAACAAAGGAAGACGGACATCAGGCAAAACCCATCAGCTTTTGGGCCAGCTTTGCTTTGCAGTTTGTGAACGTCAAAATc                                                                                      >  2:577381/1‑149 (MQ=255)
                                                      cGGTACACCTTTTGAGTTGGGCGGGGGCGGCATATATTGTCTGGCTGGCGTGGAAAATCGCCACCAGCCCAACAAAGGAAGACGGACATCAGGCAAAACCAATCAGCTTTTGGGCCCGCTTTGCTTTGCAGTTTGTGAACGTCAATATc                                                                                      >  2:579878/1‑149 (MQ=255)
                                                        gTACACCTTTTGAGTTGGGCGGGGGCGGCATATATTGTCTGGCTGGCGTGGAAAATCGCCACCAGCCCAACAAAGGAAGACGGACTTCAGGCAAAGCCAATCAGCTTTTGGGCCAGCTTTGCTTTGCAGTTTGTGATCGGCAAAATCAt                                                                                    >  1:883106/1‑149 (MQ=255)
                                                        gTACACCTTTTGAGTTGGGCGGGGGCGGCATATATTGTCTGGCTGGCGTGGAAAATCGCCACCAGCCCAACAAAGGAAGACGGACTTAAGGCAAAACCAATCAGCTTTTGGGCCAGCTTTGCTTTGCAGTTTGTGAACGTCAAAATCAt                                                                                    >  1:937472/1‑149 (MQ=255)
                                                         tACACCTTTTGAGTTGGGCGGGGGCGGCATATATTGTCTGGCTGGCGTGGAAAATCGCCACCAGCCCAACAAAGGAAGACGGACTTCAGGCAAAACCAATCAGCTTTTGGGCCAGCTTTGCTTTGCAGTTTGTGAACGTCAAAATCAtt                                                                                   >  1:428197/1‑149 (MQ=255)
                                                            acCTTTTGAGTTGGGCGGGGGCGGCATATATTGTCTGGCTGGCGTGGAAAATCGCCTCCAGCCCAACAAAGGAAGAAGGACTTCATGCAAAACCAATCAGCTTTTGGGCCAGCTTTGCTTTGCAGTTTGTGAACGTCAAAATCATTTTg                                                                                <  1:740908/149‑1 (MQ=255)
                                                              cTTTTGAGTTGGGCGGGGGCGGCATATATTGTCTGGCTGGCGTGGAAAATCGCCACCAGCCCAACAAAGGAAGACGGACTTCAGGCAAAACCAATCAGCTTTTGGGCCAGCTTTGCTTTGCAGTTTGTTAACGTCAAAATCATTTTGTa                                                                              >  2:421164/1‑149 (MQ=255)
                                                                 ttGAGTTGGGCGGGGGCGGCATATATTGTCTGGCTGGCGTGGAAAATCGCCACCAGCCCAACAAAGGAAGACGGACTTCAGGCAAAACCAATCAGCTTTTGGGCCAGCTTTGCTTTGCAGTTTGTGAACGTCAAAATCATTTTGTACgg                                                                           >  1:716232/1‑149 (MQ=255)
                                                                  tGAGTTGGGCCGGGGCGGCATATATTGTCTGGCTGGCGTGGAAAATCGCCACCAGCCCAACAAAGGAAGACGGACTTCAGTCAAAACCACTCAGCTTTTGGGCCAGCTTTGCTTTGCAGTTTGTGAACGTCAAAATCCTTTTGTACGgt                                                                          <  2:640348/149‑1 (MQ=255)
                                                                      ttGGGCGGGGGCGGCATATATTGTCTGGCTGGCGTGGAAAATCGCCACCAGCCCAACAAAGGAAGACGGCCTTCAGGCAAAACCAATCAGCTTTTGGGCCAGCTTTGCTTTGCAGTTTGTGAACGTCAAAATCATTTTGTACGGTGTTa                                                                      >  1:446261/1‑149 (MQ=255)
                                                                              gggcggCATATATTGTCTGGCTGGCGGGGAAAATCGCCACCAGCCCAACAAAGGAAGACGGACTTCATGCAAAACCAATCAGCTTTTGGGCCAGCTTTGCTTTGCAGTTTGTGAACGTCAAAATCATTTTGTACGGTGTTACGGCACTg                                                              <  1:901991/149‑1 (MQ=255)
                                                                                   gcATATATTGTCTGGCTGGCGTGGAAAATCGCCCCCAGCCCAACAAAGGAAGCCGGACATCAAGCAAAACCAATCAGCTTTTGGGCCCGCTTTTCTTTGCATTTTGTGAACGTCAAAATCATTTTGTACGGTGTTACGGCACTGTCGAc                                                         >  2:568651/1‑149 (MQ=255)
                                                                                        tatTGTCTGGCTGGCGTGGAAAATCGCCACCCGCCGAACAAAGGAAGACGGACTTCAGGCAAAAGCAATCAGCTTTTGGGCCAGCTCTGCTTTGCTGGTTGTGACCGTCAAAATCATTTTGTTCGGTGTTACGGCACGGTCGACGttgg                                                    >  1:658435/1‑147 (MQ=255)
                                                                                        tatTGTCTGGCTGGCGTGGAAAATCGCCACCAGCCCAACAAAGGAAGACGGACTTCAGGCAAAACCAATCAGCTTTTGGGCCAGCTTTGCTTTGCAGTTTGTGAACGTCAAAATCATTTTGTACGGTGTTACGGCACTGTCGACGTTTg                                                    <  2:28161/149‑1 (MQ=255)
                                                                                         atTGTCTGGCTGGCGTTGAAAATCGCCACCAGCCCAACAAAGGAAGACGGACTTCAGGCAAAACCAATCAGCTTTTGGGCCAGCTTTGCTTTGCAGTTTGTGAACGTCAAAATCATTTTGTACGGTGTTACGGCACTGTCGACGTTTGt                                                   <  2:736505/149‑1 (MQ=255)
                                                                                         atTGTCTGGCTGGCGTGGAAAATCGCCACCAGCCCAACAAAGGAAGACGGACATCAGGCAAAACCAATCAGCTTTTGGGCCAGCTTTGCTTTGCAGTTTGTGAACGTCAAAATCATTTTGTACGGTGGTACGGCACTGTCGACGTTTGt                                                   <  1:577381/149‑1 (MQ=255)
                                                                                         atTGTCGGGCTGTCGAGCAAAATCTCCACCAGCCCAACAAAGGAAGACGGTCATCAGGCAAAACCAATCAGCTTTTGGGCCAGCTGTGCTTTGCAGTTTGTGAACGTCAAAATCATTTTGTACGGTGTTACGGCACTGTCGACGTTTGt                                                   <  1:579878/149‑1 (MQ=255)
                                                                                                ggctggcGTGGAAAATCGCCACCAGCCCAACAAAGGAAGACGGACTTCAGGCAAAACCAATCAGCTTTTGGGCCAGCTTTGCTTTGCAGTTTGTGAACGTCAAATTCATTTTTTACGGTGTTACGGCACTGTCGACGTTTGTTCTGCCg                                            >  2:2246/1‑149 (MQ=255)
                                                                                                     gcGTGGAAAATCGCCACCAGCCAAACAAAGGAAGACGGACTTCAGGCAAAACCAATCAGCTTTTGGGCCAGCTTTGCTTTGCAGTTTGTGAACGTCAAAATCATATTGTACGGTGTTACGGAACTGACGACGATTTTTCTGCCGCaaaa                                       >  2:461509/1‑148 (MQ=255)
                                                                                                        tGGAAAATCGCCACCAGCCCAACAAAGGAAGACGGACTTCAGGCAAAACCAATCAGCTTTTGGGCCAGCTTTGCTTTGCAGTTTGTGAACGTCAAAATCATTTTGTACGGTGTTACGGCACTGTCGACGTTTGTTCTGCCGCAAAcaca                                    <  1:512042/149‑1 (MQ=255)
                                                                                                         ggAAAATCGCCACCAGCCCAACAAAGGAAGACGGACTTCAGGCAAAACCAATCAGCTTTTGGTCCAGCTTTGCTTTGCTGTTTGTGAACGTCAAAATCATTTTGTACGGTGTTACGGCACTGTCGACGTTTGTTCTGCCGCAAACACAg                                   >  1:432822/1‑149 (MQ=255)
                                                                                                            aaaTCGCCACCAGCCCAACAAAGGAAGACGGACTTCAGGCAATACCATTCAGCTTTTGGGCCAGCTTTGCTTTGCAGTTTGTGACCGTCAAAATGATTTTGTAGGGTGTTACGGCCCTGTCGACGTTTGTTCTGCCGCAAACACAGGCg                                >  2:207310/1‑149 (MQ=255)
                                                                                                            aaaTCGCCACCAGCCCAACAAAGGAAGACGGACTTCAGGCAAAACCAATCAGCTTTTGGGCCAGCTTTGCTTTGCAGTTTGTGAACGTCAAAATCATTTTGTACGGTGTTACGGCACTGTCGACGTTTGTTCTGCCGCAAACACAGGCg                                >  2:522335/1‑149 (MQ=255)
                                                                                                              aTCGCCACCAGCCCAACAAAGGAAGACGGACTTCAGGCAAAACCAATCAGCTTTTGGGCCAGCTTTGCTTTGCAGTTTGTGAACGTCAAAATCATTTTGTACGGTGTTACGGCACTGTCGACGTTTGTTCTGCCGCAAACACAGGCGtt                              <  2:14269/149‑1 (MQ=255)
                                                                                                               tCGCCACCAGCCCAACAAAGGAAGACGGACTTCAGGCAAAACCAATCAGCTTTTGGGCCAGCTTTGCTTTGCAGTTTGTGAACGTCAAAATCATTTTGTACGGTGTTACGGCACTGTCGACGTTTGTTCTGCCGCAAACACAGGCGTTa                             <  1:945172/149‑1 (MQ=255)
                                                                                                                cGCCTCCAGCCCAACAAAGGTAGACGGACTTCAGGCAAAACCAATCAGCTTTTGGGCCAGCTTTGCTTTGCAGTTTGTGAACGTCAAAATCATTTTGTACGGTGTTACGGCACTGTCGACGTTTGTTCTGCCGCAAACACAGGCGTTaa                            <  2:215998/149‑1 (MQ=255)
                                                                                                                cGCCACCAGCCCAACAAAGGAAGACGGACTTCAGGCAAAACCAATCAGCTTTTGGGCCAGCTTTGCTTTGCAGTTTGTGAACGTCAAAATCATTTTGTACGGTGTTACGGCACTGTCGACGTTTGTTCTGCCGCAAACACAGGCGTTaa                            <  2:154259/149‑1 (MQ=255)
                                                                                                                cGCCACCAGCCCAACAAAGGAAGACGGACTTCAGGCAAAACCAATCAGCTTTTGGGCCAGCTTTGCTTTGCAGTTTGTGAACGTCAAAATCATTTTGTACGGTGTTACGGCACTGTCGACGTTTGTTCTGCCGCAAACACAGGCGTTaa                            <  2:786962/149‑1 (MQ=255)
                                                                                                                 gCCACCAGCCCAACAAAGGAAGACGGACTTCAGGCAAAACCAATCAGCTTTTGGGCCAGCTTTGCTTTGCAGTTTGTGAACGTCAAAATCATTTTGTACGGTGTTACGGCACTGTCGACGTTTGTTCTGCCGCAAACACAGGCGTTAAg                           <  2:17069/149‑1 (MQ=255)
                                                                                                                     ccaGCCCAACAAATGAAGACGGACTTCAGGCAAAACCAATCAGCTTTTGGGCCAGCTTTGCTTTGCAGTTTGTGAACGTCAAAATCATTTTGTACGGTGTTACGGCACTGTCGACGTTTGTTCTGCCGCAAACACAGGCGTTAAGCTgg                       <  1:54743/149‑1 (MQ=255)
                                                                                                                         cccAACAAAGGAAGACGGACTTCAGGCAAAACCAATCAGCTTTTGGGCCAGCTTTGCTTTGCAGTTTGTGAACGTCAAAATCATTTTGTACGGTGTTACGGCACTGTCGACGTTTGTTCTGCCGCAAACACAGGCGTTAAGCTGGGGAg                   >  1:355121/1‑149 (MQ=255)
                                                                                                                            aacaaAGGAAGACGGACTTCAGGCAAAACCAATCAGCTTTTGGGCCAGCTTTGCTTTGCAGTTTGTGAACGTCAAAATCATTTTGTACGGTGTTACGGCACTGTCGACGTTTGTTCTGCCGCAAACACAGGCGTTAAGCTGGGTAGTTg                <  1:270568/149‑1 (MQ=255)
                                                                                                                            aacaaAGGAAGACGGACTTCAGGCAAAACCAATCAGCTTTTGGGCCAGCTTTGCTTTGCAGTTTGTGAACGTCAAAATCATTTTGTACGGTGTTACGGCACTGTCGACGTATGTTCTGCCGCAAACACAGGCGTTAAGCTGGGTAGTTg                >  2:599122/1‑149 (MQ=255)
                                                                                                                                      gACGGACTTCAGGCAAAACCAATCAGCTTTTGGGCCAGCTTTGCTTTGCAGTTTGTGAACGTCAAAATCATTTTGTACGGTGTTACGGCACTGTCGACGTTTGTTCTGCCGCAAACACAGGCGTTAAGCTGGGTAGTTGGCGTCAGCGt      >  2:728804/1‑149 (MQ=255)
                                                                                                                                        cGGCCTTCAGGCAAAACCAATCCGCTTTTGGGCCAGCTTTGCTTTGCACTTTGTGAACGGCAAAATCATTGTGTACGGTGTTACGGCACTGTCGACGTTTGTTCTGCCGCAAACACAGGCGTTAAGCGGGGTAGTTGGCGTCAGCGttt    <  2:180461/149‑1 (MQ=255)
                                                                                                                                          gACTTCAGGCAAACCCACTCAGCTGTTGGGCCAGCTTTGCTTTGCAGTTTGTGAACGTCAAAATCATTTTGTACGGTGTTACGGCACTGTCGACGTTTGTTCTGCCGCAAACACAGGCGTTAAGCTGGGTAGTTGGCGTCAGCGTTTTg  <  2:90913/149‑1 (MQ=255)
                                                                                                                                             |                                                                                                                                                 
TGATGTTACTGTGTGCGGGCATTTCATTTTCACTGGCAGTGATTGACCCGGCAGCGGTACACCTTTTGAGTTGGGCGGGGGCGGCATATATTGTCTGGCTGGCGTGGAAAATCGCCACCAGCCCAACAAAGGAAGACGGACTTCAGGCAAAACCAATCAGCTTTTGGGCCAGCTTTGCTTTGCAGTTTGTGAACGTCAAAATCATTTTGTACGGTGTTACGGCACTGTCGACGTTTGTTCTGCCGCAAACACAGGCGTTAAGCTGGGTAGTTGGCGTCAGCGTTTTG  >  NC_000913/2715580‑2715866

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑