Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,731,580 C→T 8.8% intergenic (‑423/+20) rrsG ← / ← clpB 16S ribosomal RNA of rrnG operon/protein disaggregation chaperone

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,731,5800CT 8.8% 133.5 / 3.8 56intergenic (‑423/+20)rrsG/clpB16S ribosomal RNA of rrnG operon/protein disaggregation chaperone
Reads supporting (aligned to +/- strand):  ref base C (26/25);  new base T (2/3);  total (28/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGTTGTTCCGTGTCAGTGGTGGCGCATTATAGGGAGTTATTCCGGCCTGACAAGCGAAAAATATAAAAACTTTATCGTTCGCTCACTTTTCAGGCAAAACATCTTAAATATAGTCTTTTCCGTCTAACTTATAGACAAAAACGAGCCCCGAAGGGCTCGTTTTATCATTTACTGGACGGCGACAATCCGGTCTTCATTAACTTCCAGGCGAATCACTTTACCCGGAACCAATTCACCAGACAGTATTTGCTGTGCCAGCGGGTTTTCGATCTGCTGCTGAATTGCACGTTTCAGAGG  >  NC_000913/2731432‑2731728
                                                                                                                                                    |                                                                                                                                                    
cGTTGTTCCGTGTCAGTGGTGGCGCATTATAGGGAGTTATTCCGGCCTGACAAGCGAAAAATATAAAAACTTTATCGTTCGCTCACTTTTCAGGCAAAACATCTTAAATATAGTCTTTTCCGTCTAACTTATAGACAAAAACGAGcccc                                                                                                                                                      >  2:285167/1‑149 (MQ=255)
 gttgttCCGTGTCAGTGGTGGCGCATTATAGGGAGTTATTCCGGCCTGACAAGCGAAAAATATAAAAACTTTATCGTTCGCTCACTTTTCAGGCAAAACATCTTAAATATAGTCTTTTCCGTCTAACTTATAGACAAAAACGAGCCCCg                                                                                                                                                     >  2:263352/1‑149 (MQ=255)
   tgttCCGTGTCAGTGGTGGCGCATTATAGGGAGTTATTCCGGCCTGACAAGCGAAAAATATAAAAACTTTATCGTTCGCTCACTTTTCAGGCAAAACATCTTAAATATAGTCTTTTCCGTCTAACTTATAGACAAAAACGAGCCCCGaa                                                                                                                                                   <  2:139634/149‑1 (MQ=255)
         gtgtCAGTGGTGGCGCATTATAGGGAGTTATTCCGGCCTGACAAGCGAAAAATATAAAAACTTTATCGTTCGCTCACTTTTCAGGCAAAACATCTTAAATATAGTCTTTTCCGTCTAACTTATAGACAAAAACGAGCCCCGAAGGGCTc                                                                                                                                             <  2:917352/149‑1 (MQ=255)
            gcAGTGGTGGCGCATTATAGGGAGTTATGCCGGCCTTACAAGCGAAAAATATAAAAACTTTATCGTTCGCTCACTTTTCAGGCAAAACATCTTAAATATAGTCTTTTCCGTCTAACTTATAGACAAAAACGAGCCCCGAAGGGCTCGtt                                                                                                                                          <  1:607225/148‑1 (MQ=255)
               gtggtgGCGCATTATAGGGAGTTATTCCGGCCTGACAAGCGAAAAATATAAAAACTTTATCGTTCGCTCACTTTTCAGGCAAAACATCTTAAATATAGTCTTTTCCGTCTAACTTATAGACAAAAACGAGCCCTTCGGGGCTCGttttt                                                                                                                                       <  2:893519/149‑2 (MQ=255)
               gtggtgGCGCAATATAGGGAGTTATTCCGGCCTGACAAGCGAAAAATATAAAAACTTTATCGTTCGCTCACTTTTCAGGCAAAACATCTTAAATATAGTCTTTTCCGTCTAACTTATAGACAAAAACGAGCCCTTCGGGGCTCGttttt                                                                                                                                       <  1:507072/149‑2 (MQ=39)
                 ggtggCGCATTATAGGGAGTTATTCCGGCCTGACAAGCGAAAAATATAAAAACTTTATCGTTCGCTCACTTTTCAGGCAAAACATCTTAAATATAGTCTTTTCCGTCTAACTTATAGACAACAACGAGCCCCGAAGGGCTCGTTTTATc                                                                                                                                     <  1:401311/149‑1 (MQ=255)
                  gtggCGCATTATAGGGAGTTATTCCGGCCTGACAAGCGAAAAATATAAAAACTTTATCGTTCGCTCACTTTTCAGGCAAAACATCTTAAATATCGTCTTTTCCGTCTAACTTATAGACAAAAACGAGCCCTTCGGGGCTCGtttttgtc                                                                                                                                    <  2:874015/149‑5 (MQ=39)
                    ggCGCATTATAGGGAGTTATTCCGGCCTGACAAGCGAAAAATATAAAAACTTTATCGTTCGCTCACTTTTCAGGCAAAACATCTTAAATATAGTCTTTTCCGTCTAACTTATAGACAAAAACGAGCCCCGAAGGGCTCGTTTTATCAtt                                                                                                                                  <  1:538113/149‑1 (MQ=255)
                     gcgcATTATAGGGAGTTATTCCGGCCTGACAAGCGAAAAATATAAAAACTTTATCGTTCGCTCACTTTTCAGGCAAAACATCTTAAATATAGTCTTTTCCGTCTAACTTATAGACAAAAACGAGCCCCGAAGGGCTCGTTTTATCAttt                                                                                                                                 >  1:239350/1‑149 (MQ=255)
                     gcgcATTATAGGGAGTTATTCCGGCCTGACAAGCGAAAAATATAAAAACTTTATCGTTCGCTCACTTTTCAGGCAAAACATCTTAAATATAGTCTTTTCCGTCTAACTTATAGACAAAAACGAGCCCCGAAGGGCTCGTTTTATCAttt                                                                                                                                 >  2:60002/1‑149 (MQ=255)
                       gcATTATAGGGAGTTATTCCGGCCTGACAAGCGAAAAATATAAAAACTTTATCGTTCGCTGACTTTTCAGGCAAAACATCTTAAATATAGTCTTTTCCGTCTAACTTATAGACAAAAACGAGCCCCGAAGTGATCGTTTTATCATTTaa                                                                                                                               >  1:460236/1‑148 (MQ=255)
                         aTTATAGGGAGTTATTCCGGCCTGACAAGCGAAAAATATAAAAACTTTATCGTTCGCTCACTTTTCAGGCAAAACATCTTAAATATAGTCTTTTCCGTCTAACTTATAGACAAAAACGAGCCCCGAAGGGCTCGTTTTATCATTTACTg                                                                                                                             <  2:27739/149‑1 (MQ=255)
                           tataGGGAGTTATTCCGGCCTGACAAGCGAAAAATATAAAAACTTTATAGTTCGCTCAATTTTCAGGCAAAACATCTTAAATATAGTCTTTTCCGACTAACTTATAGACAAAAACGAGCCCCGAAGGGCTCGTTTTATCATTTACTGGa                                                                                                                           <  2:110806/149‑1 (MQ=255)
                                    ttATTCCGGCCTGACAAGCGAAAAATATAAAAACTTTATCGTTCGCTCACTTTTCAGGCAAAACATCTTAAATATAGTCTTTTCCGTCGAACTTATAGACAAAAACGAGCCCCGAAGGGCTCGTTTTATCATTTACTGGACGGCGACaa                                                                                                                  >  2:869429/1‑149 (MQ=255)
                                               tGACAAGCGAAAAATATAAAAACTTTATCGTTCGCTCACTTTTCAGGCAAAACATCTTAAATATAGTCTTTTCCGTCTAACTTATAGACAAAAACGAGCCCCGAAGGGCTCGTTTTATCATTTACTGGACGGCGACAATCCGGTCTTCa                                                                                                       <  2:130540/149‑1 (MQ=255)
                                                gaaaaGCGAAAAATATAAAAACTTTATCGTTCGCTCACTTTTCAGGCAAAACATCTTAAATATAGTCTTTTCCGTCTAACTTATAGACAAAAACGAGCCCCGAAGGGCTCGTTTTATCATTTACTGGACGGCGACAATCCGGTCTTCAt                                                                                                      <  2:995074/146‑1 (MQ=255)
                                                 aCAAGCCAAAAATATAAAAACTTTATCGTGCGCTCACTTTTCAGGCAAAACATCTTAAATATAGTCTTTTCCGTCTAACTTATAGACAAAAACGAGCCCCGAAGGGCTCGTTTTATCATTTACTGGACGGCGACAATCCGGTCTTCAtt                                                                                                     <  1:698442/149‑1 (MQ=255)
                                                     gCGAAAAATATAAAAACTTTATCGTTCGCTCACTTTTGCGGCAAAACATCTTAAATATAGTGTTTTCCGTCTAACTTATAGACAAAAACGAGCCCCGAAGGGCTCGTTTTATCATGTTCTGGACGGCGACAAGCCGGGGGTCATTAACt                                                                                                 >  2:163470/1‑149 (MQ=255)
                                                     gCGAAAAATATAAAAACTTTATCGTTCGCTCACTTTTCAGGCAAAACATCTTAAATATAGTCTTTTCCGTCTAACTTATAGACAAAAACGAGCCCCGAAGGGCTCGTTTTATCATTTACTGGACGGCGACAATCCGGTCTTCATTAACt                                                                                                 >  2:910109/1‑149 (MQ=255)
                                                        aaaaaTATAAAAACTTTATCGTTCGCTCACTTTTCAGGCAAAACATCTTAAATATAGTCTTTTCCGTCTAACTTATAGACAAAAACGAGCCCCGAAGGGCTCGTTTTATCATTTACTGGACGGCGACAATCCGGTCTTCATTAACTTcc                                                                                              >  1:995939/1‑149 (MQ=255)
                                                        aaaaaTATAAAAACTTTATCGTTCGCTCACTTTTCAGGCAAAACATCTTAAATATAGTCTTTTCCGTCTAACTTATAGACAAAAACGAGCCCCGAAGGGCTCGTTTTATCATTTACTGGACGGCGACAATCCGGTCTTCATTAACTTcc                                                                                              >  2:367416/1‑149 (MQ=255)
                                                        aaaaaTATAAAAACTTTATCGTTCGCTCACTTTTCAGGCAAAACATCTTAAATATAGTCATTTCCGTCTAACTTATAGACAAAAACGAGCCCCGAAGGGCTCGTTTTATCATTTACTGGACGGCGACAATCCGGTCTTCATTAACTTcc                                                                                              <  1:287481/149‑1 (MQ=255)
                                                         aaaaTATAAAAACTTTATCGTTCGCTCACTTTTCAGGCAAAACATCTTAAATATAGTCTTTTCCGTCTAACTTATAGACAAAAACGAGCCCCGAAGGGCTCGTTTTATCATTTACTGGACGGCCACAATCCGGTCTTCATTACCTTCCa                                                                                             >  2:706656/1‑149 (MQ=255)
                                                         aaaaTATAAAAACTTTATCGTTCGCTCACTTTTCAGGCAAAACATCTTAAATATAGTCGTTTCCGGCTAACTTATAGACAAAAACGAGCCCCGAAGGGCTCGTTTTATCATTTACTGGACGGCGACAATCCGGTCTTCATTAACTTCCa                                                                                             >  2:505375/1‑149 (MQ=255)
                                                              ataAAAACTTTATCGTTCGCTCACTTTTCAGGCAAAACATCTTAAATATAGTCTTTTCCGTCTAACTTATAGACAAAAACGAGCCCCGAAGGGCTCGTTTTATCATTTACTGGACGGCGACAATCCGGTCTTCATTAACTTCCAGGCGa                                                                                        <  1:60002/149‑1 (MQ=255)
                                                                 aaaaCTTTATCGTTCGCTCACTTTTCAGGCAAAACATCTTAAATATAGTCTTTACCGTCTAACTTATAGACAAAAACGAGCCCCGAAGGGCTCGTTTTATCATTTACTGGACGGCGACAATCCGGTCTTCATTAACTTCCAGGCGAATc                                                                                     <  1:564788/149‑1 (MQ=255)
                                                                    aCTATATTGTTCGCTCACTTTTCTGGCAAAACATCTTAAATTTTGTCTATTCCGTCTATCTTATTGTCAAAAACGAGCCCCGAAGGGCTCGTTTTTTCATTTACTGGTCGGCGACAATCCGGTCTTCATTAACTTCCAGGCGAATCaaa                                                                                  <  2:751799/149‑3 (MQ=255)
                                                                      tttATCGTTCGCTCACTTTTCAGGCAAAACATCTTAAATATAGTCTTTTCCGTCTAACTTATAGACAAAAACGACCCCCGAAGGGCTCGTTTTATCATTTACTGGACGGCGACAATCCGGTCTTCATTAACTTCCAGGCGAATCACttt                                                                                <  1:883803/149‑1 (MQ=255)
                                                                         aTCGTTCGCTCACTTTTCAGGCAAAACATCTTAAATATAGTCTTTTCCGTCTAACTTATAGACAAAAACGAGCCCCGAATGGCTCGTTTTATCATTTACTGGACGGCGACAATCCGGTCTTCATTAACTTCCAGGCGAATCACTTTAcc                                                                             <  1:230573/149‑1 (MQ=255)
                                                                          tCGTTCGCTCACTTTTCAGGCAAAACATCTTAAATATAGTCTTTTCCGTCTAACTTATAGACAAAAACGCGCCCCGAAGGGCTCGTTTTATCATTTACTGGCCGGCGACAATCCTGTCTTCATTAACTTCCTGGCGAATCACTTTAccc                                                                            >  2:1006833/1‑149 (MQ=255)
                                                                               cGCTCACTTTTCAGGCAAAACATCTTAAATATAGTCTTTTCCGTCTAACTTATAGACAAAAACGAGCCCCGAAGGGCTCGTTTTATCATTTACTGGACGGCGACAATCCGGTCTTCATTAACTTCCAGGCGAATCACTTTACCCGGAAc                                                                       <  1:285167/149‑1 (MQ=255)
                                                                               cGCTCACTTTTCAGGCAAAACATCTTAAATAAAGTCTTTTCCGTCTAACTTATAGACAAAAACGAGCCCCGAAGGGCTCGTTTTATCATTTACTGGACGGCGACAATCCGGTCTTCATTAACTTCCAGGCGAATCACTTTACCCGGAAc                                                                       <  2:990578/149‑1 (MQ=255)
                                                                                gCTCACTTTTCAGGCAAAACATCTTAAATATAGTCTTTTCCGTCTAACTTATAGACAAAAACGAGCCCCGAAGGGCTCGTTTTATCATTTACTGGACGGCGACAATCCGGTCTTCATTAACTTCCAGGCGAATCACTTTACCCGGAAcc                                                                      >  1:819339/1‑149 (MQ=255)
                                                                                      ttttCAGGCATATCATCTTAAATATAGTCTTTTCCGTCTAACTTATATACAAAAACGAGCCCCGAAGGGCTCGTTTTATCATATACTGGACGGCGACAATCCGGTCTTCATTAACTTCCAGGCGAATCACTTTACCCGGAACCAATTCa                                                                <  2:584627/149‑1 (MQ=255)
                                                                                      ttttCAGGCAAAACATCTTAAATATAGTCTTTTCCGTCTAACTTATAGACAAAAACGAGCCCCGAAGGGCTCGTTTTATCATTTACTGGACGGCGACAATCCGGTCTTCATTAACTTCCAGGCGAATCACTTTACCCGGAACCAATTCa                                                                <  1:491063/149‑1 (MQ=255)
                                                                                             gCAAAACATCTTAAATATAGTCTTTTCCGTCTAACTTATAGACAAAAACGAGCCCCGAAGGGCTCGTTTTATCATTTACTGGACGGCGACAATCCGGTCTTCATTAACTTCCAGGCGAATCACTTTACCCGGAACCAATTGACCAGACa                                                         >  1:893972/1‑149 (MQ=255)
                                                                                                    aTCTTAAATATAGTCTTTTCCGTCTAACTTATAGACAAAAACGAGCCCCGAAGGGCTCGTTTTATCATTTACTGGACGGCGACAATCCGGTCTTCATTAACTTCCAGGCGAATCACTTTACCCGGAACCAATTCACCAGACAGTATTTg                                                  <  2:838063/149‑1 (MQ=255)
                                                                                                       ttAAATATAGTCTTTTCCGTCTAACTTATAGACAAAAACGAGCCCCGAAGGGCTCGTTTTATCATTTACTGGACGGCGACAATCAGGTCTTCATTAACTTCCAGGCGAATCACTTTACCCGGAACCAATTCACCAGACAGTATTTGCtg                                               >  1:638501/1‑149 (MQ=255)
                                                                                                        tAAATATAGTCTTTTCCGTCTAAATTATAGCCAAAAACGAGCCCCGAAGGGCTCGTTTTATCATTTACTGGACGGCGACAATCCGGTCTTCATTAACTTCCAGGCGAATCACTTTACCCGGAACCAATTCACCAGACAGTATTTGCtgt                                              <  2:451416/149‑1 (MQ=255)
                                                                                                               aGTCTTTTCCGTCTAACTTATAGACAAAAACGAGCCCCGAAGGGATCGTTTTATCATTTACTGGACGGCGACGATCCGGTCTTCATTAACTTCCAGGCGAATCACTTTACCCGGAACCAAGTCACCAGACAGTATTTGCTGTGCCAGCg                                       >  2:993755/1‑149 (MQ=255)
                                                                                                                 tCTTTTCCGTCTAACTTATAGACAAAAACGAGCCCCGAAGGGCTCGTTTTATCATTTACTGGACGGCGACAATCCGGTCTTCTTTAACTTCCAGGCGAATCACTTTACCCGGAACCAATTCACCAGACAGTTTTTGCTGTGCCAGCtgg                                     >  2:702696/1‑149 (MQ=255)
                                                                                                                     ttCCGTCTAACTTATAGACAAAAACGAGCCCCGAAGGGCTCGTTTTATCATTTACTGGACGGCGACAATCCGGTCTTCATTAACTTCCAGGCGAATCACTTTACCCGGAACCAATTCACCAGACAGTATTTGCTGTGCCAGCGGGgttt                                 <  2:995939/149‑1 (MQ=255)
                                                                                                                         gTCTAACTTATAGACAAAAACGAGCCCCGAAGGGCTCGTTTTATCATTTACTGGACGGCGACAATCCGGTCTTCATTAACTTCCAGGCGAATCACTTTACCCGGAACCAATTCACCAGACAGTATTTGCTGTGCCAGCGGGTTTTCGAt                             <  2:239350/149‑1 (MQ=255)
                                                                                                                         gTCTAACTTATAGACAAAAACGAGCCCCGAAGGGCTCGTTTTATCATTTACTGGACGGCGACAATCCGGTCATCATTAACTTCCAGGCGAATCACTTTACCCGGAACCAATTCCCCAGACAGTATTTGCTGTGCCAGCGGGTTTTCGAt                             >  2:833477/1‑149 (MQ=255)
                                                                                                                          tCTAACTTATAGACAAAAACGAGCCCCGAAGGGCTCGTTTTATCATTTACTGGACGGCGACAATCCGGTCTTCATTAACTTCCAGGCGAATCACTTTACCCGGAACCAATTCACCAGACAGTATTTGCTGTGCCAGCGGGTTTTCGATc                            >  2:505826/1‑149 (MQ=255)
                                                                                                                              aCTTATAGACAAAAACGAGCCCCGAAGGGCTCGTTTTATCATTTACTGGACGGCGACAATCCGGTCTTCATTAACTTCCAGGCGAATCACTTTACCCGGAACCAATTCACCAGACAGTATTTGCTGTGCCAGCGGGTTTTCGATctgct                        >  2:407916/1‑149 (MQ=255)
                                                                                                                                ttATAGACAAAAACGAGCCCCGAAGGGCTCGTGTTATCATTTACTGGACGGCGACAATCCGGTCTTCATTAACTTCCAGGCGAATCACTTTACCCGGAACCAATTCACCAGACAGTATTTGCTGTGCCAGCGGCTTTTCGATctgctgc                      >  1:716256/1‑149 (MQ=255)
                                                                                                                                      aCAAAAACGAGCCCCGAAGGGCTCGTTTTATCATTTACTGGACGGCGACAATCCGGTCTTCATTAACTTCCAGGCGAATCACTTTACCCGGAACCAATTCACCAGACAGTATTTGCTGTGCCAGCGGGTTTTCGATCTGCTGCTGAAtt                >  2:651525/1‑149 (MQ=255)
                                                                                                                                      aCAAAAACGAGCCCCGAAGGGCTCGTTTTATCATTTACTGGACGGCGACAATCCGGTCTTCATTAACTTCCAGGCGAATCACTTTACCCGGAACCAATTCACCAGACAGTATTTGCTGTGCCAGCGGGTTTTCGATCTGCTGCTGAAtt                >  2:305653/1‑149 (MQ=255)
                                                                                                                                       cAAAAACGAGCCCCGAAGGGCTCGTTTTATCATTTACTGGACGGCGACAATCCGGTCTTCATTAACTTCCAGGCGAATCACTTTACCCGGAACCAATTCACCAGACAGTATTTGCTGTGCCAGCGGGTTTTCGATCTGCTGCTGAATTg               >  2:610359/1‑149 (MQ=255)
                                                                                                                                        taaaaCGAGCCCTTCGGGGCTCGTTTTATCATTTACTGGACGGCGACAATCCGGTCTTCATTAACTTCCAGGCGAATCACTTTACCCGGAACCAATTCACCAGACAGTATTTGCTGTGCCAGCGGGTTTTCGATCTGCTGCTGAATTGc              >  2:506863/2‑149 (MQ=255)
                                                                                                                                        aaaaaCGAGCCCCGAAGGGCTCGTTTTATCATTTACTGGACGGCGACAATCCGGTCTTCATTAACTTCCAGGCGAATCACTTTCCCCGGAACCAATTCACCAGACAGTATTTGCTGTGCCAGCGGGTTTTCGATCTGCTGCTGAATTGc              >  2:659688/1‑149 (MQ=255)
                                                                                                                                        aaaaaCGAGCCCCGAAGGGCTCGTTTTATCATTTACTGGACGGCGACAATCCGGTCTTCATTAACTTCCAGGCGAATCACTTTACCCGGAACCAATTCACCAGACAGTATTTGCTGTGCCAGCGGGTTTTCGATCTGCTGCTGAATTGc              >  2:526247/1‑149 (MQ=255)
                                                                                                                                           aaCGAGCCCTTCGGGGCTCGTTTTATCATTTACTGGACGGCGACAATCCGGTCTTCATTAACTTCCCGGCGAATCACTTTACCCGGAACCAATTCACCTGACAGTATTTGCTGTGCGAGCGGGTTTTGGATCTGCTGCTGAATTGCTCg           >  1:630657/1‑149 (MQ=255)
                                                                                                                                                gCCCCGAAGGGATCGTTTTATCATTTACTGGACGGCGACGATCCGGTCTTCATTAACTTCCAGGCGAATCACTTTACCCGGAACCAATTCACCAGACAGTATTTGCAGTGCCAGCGGGTTTTCGATCTGCTGCTGAATTGCACGTTTCa      <  1:993755/149‑1 (MQ=255)
                                                                                                                                                cccccGAACGGCTCGTTTTATCATTTACTGGACGGCGACAATCCGGTCTTCATTAACTTCCAGGCGAATCACTTTACCCGGACCCAATTCACCAGACAGTATTTGCTGTGCCAGCGGGTTTTCGATCTGCTGCTGAATTGCACGTTTCa      <  2:33429/148‑1 (MQ=255)
                                                                                                                                                  cccGAAGGGCTCGTTTTATCAGTTACTGGACGGCGACAATCCGGTCTTCATTAACTTCCAGGGGAATCACTGTACACGGATCCAAGTCTCCAGACAGTATTTTCTGTGCCAGCGGGTTTTCGAGCTGTGGATGCAATGCTCGTTTCagt    >  2:1003144/1‑148 (MQ=255)
                                                                                                                                                    cGAAGGGCTCGTTTTATCATTTACTGGACGGCGACAATCGGGTCGTCTTTAACTTCCAGGCGATTCACTGTACCCGGAACCAAGTCACCAGGGGGGGGTTGCTGTGCGAGCGGGGTGTCGAGCGGCGGCGGCATTGGACGGGTCAGTgg  >  2:1020165/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
CGTTGTTCCGTGTCAGTGGTGGCGCATTATAGGGAGTTATTCCGGCCTGACAAGCGAAAAATATAAAAACTTTATCGTTCGCTCACTTTTCAGGCAAAACATCTTAAATATAGTCTTTTCCGTCTAACTTATAGACAAAAACGAGCCCCGAAGGGCTCGTTTTATCATTTACTGGACGGCGACAATCCGGTCTTCATTAACTTCCAGGCGAATCACTTTACCCGGAACCAATTCACCAGACAGTATTTGCTGTGCCAGCGGGTTTTCGATCTGCTGCTGAATTGCACGTTTCAGAGG  >  NC_000913/2731432‑2731728

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑