Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,079,618 A→G 5.8% intergenic (‑288/+26) cmtB ← / ← tktA putative mannitol‑specific enzyme IIA component of PTS/transketolase 1, thiamine triphosphate‑binding

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,079,6180AG 5.8% 273.9 / 6.7 103intergenic (‑288/+26)cmtB/tktAputative mannitol‑specific enzyme IIA component of PTS/transketolase 1, thiamine triphosphate‑binding
Reads supporting (aligned to +/- strand):  ref base A (43/54);  new base G (2/4);  total (45/58)
Fisher's exact test for biased strand distribution p-value = 6.94e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.72e-01

Only 100 of 104 total aligned reads displayed.

AGATATAACAACAAATGTCAATACGCATATCGTGATGCATATTACATAATTGATATTTATTGCATATAAATATTTGTGATCTACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATCAACAGTGAAGCCGAACTCTTCAAACAGCAGCTCTGCCGGAGCAGATTCACCGAAGGTGGTCATACCGACGATAGCACCGTTCAGGC  >  NC_000913/3079471‑3079765
                                                                                                                                                   |                                                                                                                                                   
aGATATAACAACAAATGTCAATACGCATTTCGTGATGCATATTACATAATTGATATTTATTGCATTTAAATATTTGTGATCTACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAg                                                                                                                                                    <  1:662691/149‑1 (MQ=255)
  atatAACAACAAATGTCAATACGCATATCGTGATGCATATTACATAATTGATATTTATTGCATATAAATATTTGTGATCTACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCg                                                                                                                                                  >  1:345911/1‑149 (MQ=255)
     taACAACAAATGTCAATACGCATATCTTGATGCATATTACATTATTGATATTTATTGCATATAAATATTTGTGATCTAAAACACGCATTATCTATTGCTTGTCCGCAAACGGACATATCAAGGAAATAAAAAAGTTAGCCGAAGCGAcc                                                                                                                                               >  2:751313/1‑149 (MQ=255)
      aacaacAAATGTCAATACGCATATCGTGATGCATATTACATAATTGATATTTATTGCATATAAATATTTGTGATCTACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGTTAATAAAAAAGTTCTCAGAAGAGACCt                                                                                                                                              >  2:594484/1‑149 (MQ=255)
       acaacaAATGTCAATACGCATATCGTGATGCATATTACATAATTGATATTTAATGCATATAAATATTTGTGATCTACAACACGCCTTATCTATTGCTTGTCCGCAAAAGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCtt                                                                                                                                             >  2:480919/1‑149 (MQ=255)
        caacaaATGTCAATACGCATATCGTGATGCATATTACATAATTGATATTTATTGCATATAAATATTTGTGATCTACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCTTCGGCGACCttt                                                                                                                                            <  2:733753/149‑1 (MQ=255)
        caacaaATGTCAATACGCATATCGTGATGCATATTACATAATTGATATTTATTGCATATAAATATTTGTGATCTACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCttt                                                                                                                                            >  2:935022/1‑149 (MQ=255)
         aacaaATGTCAATACGCATATCGTGATGCATATTACATAATTGATATTTATTGCATATAAATATTTGTGATCTACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCTTCGGCGACCtttt                                                                                                                                           <  1:511729/149‑1 (MQ=255)
         aacaaATGTCAATACGCATATCGTGATGCATATTACATAATTGATATTTATTGCATATAAATATTTGTGATCTACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCtttt                                                                                                                                           <  2:38295/149‑1 (MQ=255)
         aacaaATGTCAATACGCATATCGTGATGCATATTACATAATTGATATTTATTGCATATAAATATTTGTGATCTACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCAAAGCGACCtttt                                                                                                                                           <  1:867239/149‑1 (MQ=255)
          acaaATGTCAATACGCATATCGTGATGCATATTACATAATTGATATTTCTTGCATATAAATATTTGTGATCTACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCTTCGGCGACCttttt                                                                                                                                          <  1:788884/149‑1 (MQ=255)
          acaaATGTCAATACGCATATCGTGATGCATATTACATAATTGATATTTATTGCATATAAATATTTGTGATCTACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCttttt                                                                                                                                          <  2:846585/149‑1 (MQ=255)
           caaATGTCAATACGCATATCGTGATGCATATTACATAATTGATATTTATTGCATATAAATATTTGTGATCTACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCTTCGGCGACCtttttt                                                                                                                                         <  1:1005140/149‑2 (MQ=255)
           caaATGTCAATACGCATATCGTGATCCATATTACATAATTGATATTTATTGCATATAAATATTTGTGATCTACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTa                                                                                                                                         >  2:175593/1‑149 (MQ=255)
            aaaTGTCAATACGCATATCGTGATGCATATTACATAATTGATATTTATTGCATATAAATATTTGTGATCTACAACACGCCTTATCTATTGCTTGTCCGCCAACGGACATATCAAGGTAATAAAAAAGGGCGCCGAAGCGACCTTTTTAc                                                                                                                                        <  1:496376/149‑1 (MQ=255)
            aaaTGTCAATACGCATATCGTGATGCATATTACATAATTGATATTTATTGCATATAAATATTTGTGATCTACAACACGCATTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCTCCGAAGCGACCTTTTTAc                                                                                                                                        >  1:954251/1‑149 (MQ=255)
              aTGTCAATACGCATATCGTGATGCATATTACATAATTGATATTTATCGCATATAAATATTTGTGATATACAACACGCCTTATCTATTGCTTGTCCGCAAACGGAAATATCAAGGTAATAAAAAAGGTCGCCGAAGTGACCTTTTTAcac                                                                                                                                      >  1:77190/1‑147 (MQ=255)
                  cAATACGCATATCGTGATGCATATTACATAATTGATATTTATTGCATATAAATATTTGTGATCTACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGaaa                                                                                                                                  <  1:158537/149‑1 (MQ=255)
                       cGCATATCGTGATGCATATTACATAATTGATATTTATTGCATATAAATATTTGTGATCTACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTa                                                                                                                             <  1:220532/149‑1 (MQ=255)
                          atatCGTGATGCATATTACATAATTGATATTTATTGCATATAAATATTTGTGATCTACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGCCGCCGAAGCGACCTTTTTACCCGAAATGCTAAtt                                                                                                                          <  1:476774/149‑1 (MQ=255)
                          aaatCGTGATGCATATTAAATAATTGATATTTATTGAATATAAATATTTGTGATCTACAACACGCATAATATATTCCTTGTCAGCAAACGGACATAAAAAGGTAATAAAAAAGGTCGCCGAAGCTACCTTATTACAAGAAATTCTAAta                                                                                                                          >  2:380708/3‑148 (MQ=255)
                            atagttaTGCATATTACATAATTGATATTTATTGCATATAAATATTTGTGATCTACCACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTAc                                                                                                                        <  1:488181/143‑1 (MQ=255)
                            atCGTGATGCATATTACATAATTGATATTTATTGCATATAAATATTTGTGATCTACAACCCGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAACAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTAc                                                                                                                        <  2:960170/149‑1 (MQ=255)
                            atCGTGATGCATATTACATAATTGATATTTATTGCATATAAATATTTGTGATCTACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTAc                                                                                                                        <  1:1011010/149‑1 (MQ=255)
                             tCGTGATGCATATTACATAATTGATATTTATTGCATATAAATATTTGTGATCTACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTAca                                                                                                                       >  2:80734/1‑149 (MQ=255)
                              cGTGATGCATATTACATAATTGATATTTATTGCATATAAATATTTGTGATCTACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTAcag                                                                                                                      <  2:345911/149‑1 (MQ=255)
                                tGATGCATATTACATAATTGATATTTATTGCATATAAATATTTGTGATCTACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTAcagca                                                                                                                    <  2:659172/149‑1 (MQ=255)
                                tGATGCATATTACATAATTGATATTTATTGCATATAAATATTTGTGATCTACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGAGACCTTTTTACCCGAAATGCTAATTAcagca                                                                                                                    >  1:189446/1‑149 (MQ=255)
                                 gATGCATATTACATAATTGATATTTATTGCATATAAATATTTGTGATCTACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTAcagcag                                                                                                                   <  1:642181/149‑1 (MQ=255)
                                    gCATATTACATAATTGATATTTATTGCATATAAATATTTGTGATCTACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTc                                                                                                                >  1:905553/1‑149 (MQ=255)
                                     cATATTACATAATTGATATTTATTGCATATAAATATTTGTGATCTACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACACGAAATGCTAATTACAGCAGTTCt                                                                                                               >  2:644978/1‑149 (MQ=255)
                                      atatTACATAATTGATATTTATTGCATATAAATATTTGTGATCTACAACACGCCTTATCTATTGCCTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCtt                                                                                                              <  1:58369/149‑1 (MQ=255)
                                        atTACATAATTGATATTTATTGCATATAAATATTTGTGATCTACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCtttt                                                                                                            <  1:512059/149‑1 (MQ=255)
                                        atTACATAATAGATATTTATTGCATATAAATATTTGTGATCTACAACACTCCTTATCTATTGCTTGTCCGCAAACGGAAATATCAAGGTAATAAAAAAGGGCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCtttt                                                                                                            <  2:175903/149‑1 (MQ=255)
                                           aCATAATTGATATTTATTGCATATAAATATTTGTGATCTACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATAAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCt                                                                                                         >  1:115787/1‑149 (MQ=255)
                                              tAATTGATAATTATTGCATATAAATTTTTGTGATCTACAACACGCCTTATCTATTGCTTGGCCGCAAACGGACATATCAAGGTAATAAAAAAGGGCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTc                                                                                                      <  2:1015140/149‑1 (MQ=255)
                                               aaTTGCTATTTATTGCATATAAATAATTGTTATCTACAAAACGCCTTATCTTTTGCTTGTCCGCATACGGACATATCAAGGTAATACAAAAGGTCTCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTct                                                                                                     <  1:575350/149‑2 (MQ=255)
                                               aaTTGATATTTATTGCATATAAATATTTGTGATCTACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCg                                                                                                     <  1:575348/149‑1 (MQ=255)
                                                aTTGATATTTATTGCATATAAATATTTGTGATCTACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGc                                                                                                    >  1:822832/1‑149 (MQ=255)
                                                  tGATATTTATTGCATATAAATATTTGTGATCTACAACACGCCTTATCTATTGCTTGTCCTCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGcaa                                                                                                  <  1:17766/149‑1 (MQ=255)
                                                            tgaatatAAATATTTGTGATCTACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATc                                                                                        <  1:302139/146‑1 (MQ=255)
                                                            tGCATATAAATATTTGTGATCTACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATc                                                                                        <  1:889170/149‑1 (MQ=255)
                                                            tGCATATAAATATTTGTGATCTACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATc                                                                                        <  2:34863/149‑1 (MQ=255)
                                                             gCATATAAATATTTGTGATCTACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATAACAGCAGTTCTTTTGCTTTCGCAACAACGTTATCa                                                                                       >  2:518391/1‑149 (MQ=255)
                                                                tataAATATTTGTGATCTACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATCAACa                                                                                    >  1:929626/1‑149 (MQ=255)
                                                                 ataAATATTTGTGATCTACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATATAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATCAACAg                                                                                   <  2:817785/149‑1 (MQ=255)
                                                                  taAATATTTGTGATCTACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATCAACAGt                                                                                  <  1:735570/149‑1 (MQ=255)
                                                                   aaaTATTTGTGATCTACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATCAACAGTg                                                                                 <  1:935022/149‑1 (MQ=255)
                                                                   aaaTATTTGTGATCTACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATCAACAGTg                                                                                 >  1:55908/1‑149 (MQ=255)
                                                                     atatTTGTGATCTACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATCAACAGTGaa                                                                               >  2:20044/1‑149 (MQ=255)
                                                                           gtgATCTACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACTTATCAAGGTAATAAAAAAGGTCGCCGCAGCGACCTGAGTACCCGAAATGCTAAGTACCGCCGTGCTTTTGCTTACGCAACAACGTTATCAACAGTGATGCCGac                                                                         >  1:575862/1‑148 (MQ=255)
                                                                            tgATCTACAACACACCTTATCTATTGCTTGTCCTCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCTACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATCAACAGTGAAGCCGAAc                                                                        <  2:444577/149‑1 (MQ=255)
                                                                               tCTACAACTCGCTTTATGTTTTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAATGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTAGAGCAGTTCTTTTGCTTTCGCAACAACGTTATCAACAGTGAAGCCGAActct                                                                     <  2:954251/149‑1 (MQ=255)
                                                                               tCTACAACAAGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCAGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATCAACAGTGAAGCCGAActct                                                                     >  2:512190/1‑149 (MQ=255)
                                                                                cTACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATCAACAGTGAAGCCGAACTCtt                                                                    <  2:951417/149‑1 (MQ=255)
                                                                                cTACAACACGCCTTATCTAATGCTTGTCCGCAAACGGACATAACAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTAACCGAAATGCTAATTACAGCACTTCTTTTGCTTTCGCAACAACGTTATCAACAGTGAAGCCGAACTCtt                                                                    >  1:706125/1‑149 (MQ=255)
                                                                                 ttcaacaCGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATCAACAGTGAAGCCGAACTCTTc                                                                   <  2:464053/147‑1 (MQ=255)
                                                                                 tACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATCAACAGTGAAGCCGAACTCTTc                                                                   <  2:11602/149‑1 (MQ=255)
                                                                                 tACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATCAACAGTGAAGCCGAACTCTTc                                                                   <  2:799249/149‑1 (MQ=255)
                                                                                  acaacaCGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATCAACAGTGAAGCCGAACTCTTCa                                                                  <  2:142640/149‑1 (MQ=255)
                                                                                   caacaCGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATCAACAGTGAAGCCGAACTCTTCaa                                                                 >  1:154693/1‑149 (MQ=255)
                                                                                    aacaCGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATCAACAGTGAAGCCGAACTCTTCaaa                                                                >  1:810866/1‑149 (MQ=255)
                                                                                     acacGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATCAACAGTGAAGCCGAACTCTTCAAAc                                                               >  2:88778/1‑149 (MQ=255)
                                                                                       acGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATCAACAGTGAAGCCGAACTCTTCAAAcag                                                             <  1:69491/149‑1 (MQ=255)
                                                                                          ccTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATCAACAGTGAAGCCGAACTCTTCAAAcagcag                                                          <  2:651818/149‑1 (MQ=255)
                                                                                           ctgaTCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCAGAAGCGACCTTTTTACACGAATTGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATCAACAGTGAAGCCGCACTCTTCAAACagcagc                                                         >  2:280788/4‑149 (MQ=255)
                                                                                               tCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATCAACAGTGAAGCCGAACTCTTCAAACAGCAGCTCTg                                                     >  1:650207/1‑149 (MQ=255)
                                                                                                  aTTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATCAACAGTGAAGCCGAACTCTTCAAACAGCAGCTCTGCCg                                                  >  1:674680/1‑149 (MQ=255)
                                                                                                  aTTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATCAACAGTGAAGCCGAACTCTTCAAACAGCAGCTCTGCCg                                                  >  2:1005403/1‑149 (MQ=255)
                                                                                                      cTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCTGTTGTTTTGCTTTCGCAACTACGTTATCAACAGTGAAGGCGAACTCTTCAAACAGCAGCTCTGCCGGAGc                                              >  1:655059/1‑149 (MQ=255)
                                                                                                      cTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCAGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATCAACAGTGAAGCCGAACTCTTCAAACAGCAGCTCTGCCGGAgt                                              <  2:185022/149‑2 (MQ=255)
                                                                                                       ttGTCCGCATACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATCAACAGTGAAGCCGAACTCTTCAAACAGCAGCTCTGCCGGAGCa                                             <  1:589824/149‑1 (MQ=255)
                                                                                                          tCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATCAACAGTGAAGCCGACCTCTTCAAACAGCAGCTCTGCCGGAGCAGAt                                          >  2:207401/1‑149 (MQ=255)
                                                                                                             gCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATCAACAGTGAAGCCGAACTCTTCAAACAGCAGCTCTGCCGGAGCAGATTCa                                       >  1:718086/1‑149 (MQ=255)
                                                                                                             gCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATCAACAGTGAAGCCGAACTCTTCAAACAGCAGCTCTGCCGGAGCAGATTCa                                       >  2:331857/1‑149 (MQ=255)
                                                                                                              cAAACGGACATATCAAGGTATTAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTATTTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATCAACAGTGAAGCCGAACTCTTCAAACAGCAGCTCTGCCGGAGCAGATTCAc                                      <  2:164348/149‑1 (MQ=255)
                                                                                                              cAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATCAACAGTGAAGCCGAACTCTTCAAACAGCAGCTCTGCCGGAGCAGATTCAc                                      <  1:175593/149‑1 (MQ=255)
                                                                                                               aaaCGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATCAACAGTGAAGCCGAACTCTTCAAACAGCAGCTCTGCCGGAGCAGATTCAcc                                     <  2:292363/149‑1 (MQ=255)
                                                                                                               aaaCGGACATATCAAGGTAATAAAAAAGGACGCCGAAGCGACCGTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATCACCATTGAAGCCGAACTCTTCAAACAGCAGCTCTGCCGGAGCCGATTCAcc                                     >  1:913167/1‑149 (MQ=255)
                                                                                                                     aCATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTCCCCGAAATGCTAATTACAGCAGTTGTTTTGCTTTCGCACCCACGTTATCAACAGTGAAGCCGAACTCTTGAAACAGCAGCTCTGCCGGAGCAGATTCACCGAAggt                               >  1:676605/1‑149 (MQ=255)
                                                                                                                     aCATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATCAACAGTGAAGCCGAACTCTTCAAACAGCAGCTCTGCCGGAGCAGATTCACCGAAggt                               <  2:822832/149‑1 (MQ=255)
                                                                                                                      cATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATCAACAGTGAAGCCGAACTCTTCAAACAGCAGCTCTGCCGGAGCAGATTCACCGAAggtg                              <  2:674680/149‑1 (MQ=255)
                                                                                                                      aatatCAAGGTAATAAATTAGGTCGCCGAAGTGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATCAACAGTGAAGCCGAACTCTTCAAACAGCAGCTCTGCCGGAGCAGATTCACCGAAggtg                              <  2:77190/148‑1 (MQ=255)
                                                                                                                        tatCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATCAACAGTGAAGCCGAACTCTTCAAACAGCAGCTCTGCCGGAGCAGATTCACCGAAggtggt                            >  1:503538/1‑149 (MQ=255)
                                                                                                                        tatCAAGGTAATAAAAAAGGGCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATCAACAGTGAAGCCGAACTCTTCAAACAGCAGCTCTGCCGGAGCAGATTCACCGAAggtggt                            <  1:80734/149‑1 (MQ=255)
                                                                                                                         atCAAGTAAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCGAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATCAACAGTGAAGCCGAACTCTTCAAACAGCAGCTCTGCCGGAGCTGATTCACCGAAggtggta                           >  2:389913/1‑148 (MQ=255)
                                                                                                                         atCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATCAACAGTGAAGCCGAACTCTTCAAACAGCAGCTCTGCCGGAGCAGATTCACCGAAGGTGGTc                           >  2:512224/1‑149 (MQ=255)
                                                                                                                         atCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTAAAGCAGTTCTTTTGCTTTCGCAACAACGTTATCAACAGTGAAGCCGAACTCTTCAAACAGCAGCTCTGCCGGAGCAGATTCACCGAAGGTGGTc                           <  2:87830/149‑1 (MQ=255)
                                                                                                                          tCAAGGTAATAAAAAAGGTCGCCGAAGCGCCCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATCAACAGTGAAGCCGAACTCTTCAAACAGCAGCTCTGCCGGAGCAGATTCACCGAAGGTGGTCa                          >  2:887476/1‑149 (MQ=255)
                                                                                                                           cAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATCAACAGTGAAGCCGAACTCTTCAAACAGCAGct                                                        >  2:861854/1‑118 (MQ=255)
                                                                                                                           cAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATCAACAGTGAAGCCGAACTCTTCAAACAGCAGct                                                        <  1:861854/118‑1 (MQ=255)
                                                                                                                                  ggtaaaaaGGTCGCTTCGGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATCAACAGTGAAGCCGAACTCTTCAAACAGCAGCTCTGCCGGAGCAGATTCACCGAAGGTGGTCATACcgacg                  >  1:545789/4‑149 (MQ=255)
                                                                                                                                    taaaaaGGTCGCTTCGGCGACCGTTTTACCCGAAATGCTACTTACAGCAGTTCTTTTGCTTTCGCACCAACGGTATCAACAGTGAAGACGCACTCTTCAAACAGCAGCTCTGCCGGAGCAGAGTCCCCGCAGGTGGTCATACCGACGAt                >  1:472222/2‑149 (MQ=255)
                                                                                                                                    aaaaaaTGTCGACGAAGCGACCTTTTTACCCGAAATGCTAAATACAGCAGTTCTTTTGCTATCGCAACTACGTTATCAACAGTCAAGCCGAACTCTTCAAACAGCAGCTCTGCCGGAGCAGATTCACCGAAGGTGGTCATACCGACGAt                <  1:751313/149‑1 (MQ=255)
                                                                                                                                    aaaaaaGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATCAACAGTGAAGCCGAACTCTTCAAACAGCAGCTCTGCCGGAGCAGATTCACCGAAGGTGGTCATACCGACGAt                >  2:19350/1‑149 (MQ=255)
                                                                                                                                        aaGGTCGCCGAATCGACCTTTTTACCAGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATCAACAGTGATGCCGAACTCTTCAAACAGCAGCTCTGCCGGAGCAGATTCACCGAAGGTGGTCATACCGACGATAGCa            <  2:585696/149‑1 (MQ=255)
                                                                                                                                             cGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATCAACAGTGAAGCCGAACTCTTCAAACAGCAGCTCTGCCGGAGCAGATTCACCGAAGGTGGTCATACCGACGATAGCACCGtt       <  2:905553/149‑1 (MQ=255)
                                                                                                                                               ccGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATCAACAGTGAAGCCGAACTCTTCAATCAGCAGCTCTGCCGGAGCAGATTCACCGAAGGTGGTCATACCGACGATAGCACCGTTCa     >  2:813699/1‑149 (MQ=255)
                                                                                                                                                cGAAGCGACCTTTTTACCCGAATTGCTAATTACAGCAGTTCTTTTGCTTTCGCAACATCGTTATCAACAGTGAAGCCGAACTCTTCAATCAGCAGCTCTGCCGGAGCAGATTCACCGAAGGTGGTCATACCGACGATAGCACCGTTCAg    <  2:706125/149‑1 (MQ=255)
                                                                                                                                                  aaGCTACATTTTTACCAGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATCAACAGTGAAGCCGAACTCTTCAAACAGCAGCTCTGCCGGAGCAGATTCACCGAAGGTGGTCATACCGACGATAGCACCGTTCAGGc  <  1:644978/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
AGATATAACAACAAATGTCAATACGCATATCGTGATGCATATTACATAATTGATATTTATTGCATATAAATATTTGTGATCTACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATCAACAGTGAAGCCGAACTCTTCAAACAGCAGCTCTGCCGGAGCAGATTCACCGAAGGTGGTCATACCGACGATAGCACCGTTCAGGC  >  NC_000913/3079471‑3079765

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: