Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	3,095,711	C→T	6.3%	S205L (TCG→TTG)	yggS →	UPF0001 family protein, PLP‑binding

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,095,711	0	C	T	6.3%	168.5 / 2.4	64	S205L (TCG→TTG)	yggS	UPF0001 family protein, PLP‑binding
Reads supporting (aligned to +/- strand):  ref base C (34/25);  new base T (2/2);  total (37/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.87e-01

CCGCGTTTACGTCTGCGCGGGTTGATGGCAATCCCTGCGCCTGAGTCAGAATATGTAAGGCAGTTTGAAGTTGCACGCCAAATGGCTGTAGCATTTGCCGGACTGAAAACGCGCTACCCGCATATCGACACGCTCTCTCTGGGAATGTCGGACGATATGGAAGCCGCCATTGCGGCAGGTAGCACGATGGTTCGTATCGGCACTGCAATTTTTGGTGCGCGTGATTACTCTAAAAAATAAGGAATTAAAGGAACGCCATGAATACGTTGACTTTCCTGCTTTCAACGGTCATTGAGC  >  NC_000913/3095563‑3095859                                                                                                                                                     |                                                                                                                                                     ccGCGTTTACGTCTGCGCGGGTTGATGGCAATCCCTGCGCCTGAGTCAGAATATGTAAGGCAGTTTGAAGTTGCACGCCAAATGGCTGTAGCATTTGCCGGACTGAAAACGCGCTACCCGCATATCGACACGCTCTCTCTGGGAATGTc                                                                                                                                                      >  1:1027535/1‑149 (MQ=255) ccGCGTTTACGTCTGCGCGGGTTGATGGCAATCCCTGCGCCTGAGTCAGAATATGTAAGGCAGTTTGAAGTTGCACGCCAAATGGCTGTAGCATTTGCCGGACTGAAAACGCGCTACCCGCATATCGACACGCTCTCTCTGGGAATGTc                                                                                                                                                      >  1:808487/1‑149 (MQ=255) ccGCGTTTACGTCTGCGCGGGTTGATGGCAATCCCTGCGCCTGAGTCAGAATATGTAAGGCAGTTTGAAGTTGCACGCCAAATGGCTGTAGCATTTGCCGGACTGAAAACGCGCTACCCGCATATCGACACGCACTCTCTGGGAATGTc                                                                                                                                                      >  1:259280/1‑149 (MQ=255)   gcgTTTACGTCTGCGCGGGTTGATGGCAATCCCTGCGCCTGAGTCAGAATATGTAAGGCAGTTTGAAGTTGCACGCCAAATGGCTGTAGCATTTGCCGGACTGAAAACGCGCTACCCGCATATCGACACGCTCTCTCTGGGAATGTCgg                                                                                                                                                    >  1:485283/1‑149 (MQ=255)           gTCTGCGCGGGTTGATGGCAATCCCTGCGCCTGAGTCAGAATATGTAAGGCAGTTTGAAGTTGCACGCCAAATGGCTGTAGCATTTGCCGGACTGAAAACGCGCTACCCGCATATCGACACGCTCTCTCTGGGAATGTCGGACGATATg                                                                                                                                            >  1:905520/1‑149 (MQ=255)           gTCTGCGCGGGTTGATGGCAATCCCTGCGCCTGAGTCAGAATATGTAAGGCAGTTTGAAGTTGCACGCCAAATGGCTGTAGCATTTGCCGGACTGAAAACGCGCTACCCGCATATCGACACGCTCTCTCTGGGAATGTCGGACGATATg                                                                                                                                            >  1:861719/1‑149 (MQ=255)              tGCGCGGGTTGATGGCAATCCCTGCGCCTGAGTCAGAATATGTAAGGCAGTTAGAAGTTGCACGCCAAATGGCTGTAGCATTTGCCGGACTGAAAACGCGCTACCCGCATATCGACACGCTCTCTATGGGAATGTCGGACGATATGGaa                                                                                                                                         >  2:189623/1‑149 (MQ=255)               gcgcGGGTTGATGGCAATCCCTGCGCCTGAGTCAGAATATGTAAGGCAGTTTGAAGTTGCACGCCAAATGGCTGTAGCATTTGCCGGACTGAAAACGCGCTACCCGCATATCGACACGCTCTCTCTGGGAATGTCGGACGATATGGAAg                                                                                                                                        <  1:956943/149‑1 (MQ=255)                        gATGGCAATCCCTGCGCCTGAGTCAGAATATGTAAGGCAGTTTGAAGTTGCACGCCAAATGGCTGTAGCATTTGCCGGACTGAAAACGCGCTACCCGCATATCGACACGCTCTCTCTGGGAATGTCGGACGATATGGAAGCCGCCATTg                                                                                                                               <  1:865435/149‑1 (MQ=255)                           ggCAATCCCTGCGCCTGAGTCAGAATATGTAAGGCAGTTTGAAGTTGCACGCCAAATGGCTGTAGCATTTGCCGGACTGAAAACGCGCTACACGCAAATCGACACGCTCTCGCTGGGAATGTAGGACGATATGGAAGCCGCCATTGAgg                                                                                                                            >  2:949798/1‑149 (MQ=255)                           ggCAATCCAGGCGCCTGAGTCAGAATATGTAAGGCAGTTTGAAGTTGCACGCCAAATGGCTGTAGCATTTGCCGGACTGAAAACGCGCTACCCGCATATCGACACGCTCTCTCTGGGAATGTCGGACGATATGGAAGCCGCCATTGCgg                                                                                                                            <  2:462509/149‑1 (MQ=255)                            gCAATCCCTGCGCCTGAGTCAGAATATGTAAGGCAGTTTGAAGTTGCACGCCAAATGGCTGTAGCATTTGCCGGACTGAAAACGCGCTACCCGCATATCGACACGCTCTCTCTGGGAATGTCGGACGATATGGAAGCCGCCATTGCGGc                                                                                                                           >  1:258206/1‑149 (MQ=255)                            gCAATCCCTGCGCCTGAGTCAGAATATGTAAGGCAGTTTGAAGTTGCACGCCAAATGGCTGTAGCATTTGCCGGACTGAAAACGCGCTACCCGCATATCGACACGCTCTCTCTGGGAATGTCGGACGATATGGAAGCCGCCATTGCGGc                                                                                                                           <  1:937933/149‑1 (MQ=255)                             cAATCCCTGCGCCTGAGTCAGAATATGTAAGGCAGTTTGAAGTTGCACGCCAAATGGCTGTAGCATTTGCCGGACTGAAAACGCGCTACCCGCATATCGACACGCTCTCTCTGGGAATGTCGGACGATATGGAAGCCGCCATTGCGGCa                                                                                                                          >  1:396223/1‑149 (MQ=255)                                acccTGCGCCTGAGTCAGAATATGTAAGGCAGTTTGAAGTTGCACGCCAAATGGCTGTAGCATTTGCCGGACTGAAAACGCGCTACCCGCATATCGACACGCTCTCTCTGGGAATGTCGGACGATATGGAAGCCGCCATTGCGGCAGGt                                                                                                                       <  1:924628/148‑1 (MQ=255)                                  ccTGCGCCTGAGTCAGAATATGTAAGGCAGTTTGAAGTTGCACGCCAAATGGCTGTAGCATTTGCCGGACTGAAAACGCGCTACCCGCATATCGACACGCTCTCTCTGGGAATGTCGGACGATATGGAAGCCGCCATTGCGGCAGGTAg                                                                                                                     <  1:185380/149‑1 (MQ=255)                                   cTGCGCCTGAGTCAGAATATGTAAGGCAGTTTGAAGTTGCACGCCAAATGGCTGTAGCATTTGCCGGACTGAAAACGCGCTACCCGCATATCGACACGCTCTCTCTGGGAATGTCGGACGATATGGAAGCCGCCATTGCGGCAGGTAGc                                                                                                                    >  1:715800/1‑149 (MQ=255)                                    tGCGCCTGAGTCAGAATATGTAAGGCAGTTTGAAGTTGCACGCCAAATGGCTGTAGCATTTGCCGGACTGAAAACGCGCTACCCGCATATCGACACGCTCTCTCTGGGAATGTCGGACGATATGGAAGCCGCCATTGCGGCAGGTAGCa                                                                                                                   >  2:59344/1‑149 (MQ=255)                                    tGCGCCTGAGTCAGAATATGTAAGGCAGTTTGAAGTTGCACGCCAAATGGCTGTAGCATTTGCCGGACTGAAAACGCGCTACCCGCATATCGACACGCTCTCTCTGGGAATGTCGGACGATATGGAAGCCGCCATTGCGGCAGGTAGCa                                                                                                                   <  2:83348/149‑1 (MQ=255)                                          tGAGTCAGAATATGTAAGGCAGTTTGAAGTTGCACGCCAAATGGCTGTAGCATTTGCCGGACTGAAAACGCGCTACACGCATATCGACACGCTCTCTCTGGGAATGTCGGACGATATGGAAGCCGCCATTGCGGCAGGTAGCACGATgg                                                                                                             >  1:124253/1‑149 (MQ=255)                                            aGTCAGAATATGTAAGGCAGTTTGAAGTTGCACGCCAAATGGCTGTAGCATTTGCCGGACTGAAAACGCGCTACCCGCATATCGACACGCTCTCTCAGGGAATGTCGGACGATATGGAAGCCGCCATTTCGGCAGCTAGCACGATGCtt                                                                                                           >  2:135065/1‑149 (MQ=255)                                              tCAGAATATGTAAGGCAGTTTGAAGTTGCACGCCAAATGGCTGTAGCATTTGCCGGACTGAAAACGCGCTACCCGCATATCGACACGCTCTCTCTGGGAATGTCGGACGATATGGAAGCCGCCATTGCGGCAGGTAGCACGATGGTTCg                                                                                                         >  1:135372/1‑149 (MQ=255)                                               cAGAATATGTAAGGCAGTTTGAAGTTGCACGCCAAATGGCTGTAGCATTTGCCGGACTGAAAACGCGCTACCCGCATATCGACACGCTCTCTCTGGGAATGTCGGACGATATGGAAGCCGCCATTGCGGCAGGTAGCACGATGGTTCGt                                                                                                        >  1:430500/1‑149 (MQ=255)                                               cAGAATATGTAAGGCAGTTTGAAGTTGCACGCCAAATGGCTGTAGCATTTGCCGGACTGAAAACGCGCTACCCGCATATCGACACGCTCTCTCTGGGAATGTCGGACGATATGGAAGCCGCCATTGCGGCAGGTAGCACGATGGTTCGt                                                                                                        <  1:52148/149‑1 (MQ=255)                                                aGAATATGTAAGGCAGTTTGAAGTTACACGCCAAATGGCTGTAGCATTTGCCGGACTGAAAACGCGCTACCCGCATATCGACACGCTCTCTCTGGGAATGTCGGACGATATGGAAGCCGCCATTGCGGCAGGTAGCACGATGGTTCGTa                                                                                                       >  1:963094/1‑149 (MQ=255)                                                 gAATATGTAAGGCAGTTTGAAGTGGCACGCCAACTGGCTGTAGCATTTGCCGGACTGAAAACGCGCTACCCGCATATCGACACGCTCTCTCTGGGAATGTCGGACGATATGGAAGCCGCCATTGCGGCAGGTAGCACGATGGTTCGTAt                                                                                                      <  1:571953/149‑1 (MQ=255)                                                     atGTAAGGCAGTTTGAAGTTGCACGCCAAATGGCTGTAGCATTTGCCGGACTGAAAACGCGCTACCCGCATATCGACACGCTCTCTCTGGGAATGTCGGACGATATGGAAGCCGCCATTGCGGCAGGTAGCACGATGGTTCGTATCGGc                                                                                                  <  2:1027535/149‑1 (MQ=255)                                                       gTAAGGCAGTTTGAAGTTGCACGCCAAATGGCTGTAGCATTTGCCGGACTGAAAACGCGCTACCCGCATATCGACACGCTCTCTCTGGGAATGTCGGCCGATATGGCCGCCGCCATTGCGGCAGGTAGAACGATGGTTCGTATCGGCAc                                                                                                >  2:57681/1‑149 (MQ=255)                                                         aaGGCAGTTTGAAGTGGCACGCCAAATGGCTGTATCATTTGCCGGACGGAAAACGCGCTACCCGCATATCGACCCGCGCTCTCTGGGAATGACGGACGATATGGAAGCCGCCATTGCTGCAGTTAGCACGATGGTTCGTATCGGCACTg                                                                                              >  2:213467/1‑149 (MQ=255)                                                            gCAGTTTGAAGTTGCACGCCAAATGGCTGTAGCATTTGCCGGACTGAAAACGCGCTACCCGCATATCGACACGCTCTCTCTGGGAATGTCGGACGATATGGAAGCCGCCATTGCGGCAGGTAGCACGATGGTTCGTATCGGCACTGCaa                                                                                           >  2:198341/1‑149 (MQ=255)                                                            gCAGTTTGAAGTTGCACGCCAAATGGCTGTAGCATTTGCCGGACTGAAAACGCGCTACCCGCATATCGACACGCTCTCTCTGGGAATGTCGGACGATATGGAAGCCGCCATTGCGGCAGGTAGCACGATGGTTCGTATCGGCACTGCaa                                                                                           >  2:480371/1‑149 (MQ=255)                                                              aGTTTGAAGTTGCACGCCAAATGGCTGTAGCATTTGCCGGACTGAAAACGCGCTACCCGCATATCGACACGCTCTCTCTGGGAATGTCGGACGATATGGAAGCCGCCATTGCGGCAGGTAGCCCGATGGTTCGTATCGGCACTGCAAtt                                                                                         >  1:482037/1‑149 (MQ=255)                                                                tttGAAGTTGCACGCCAAATGGCTGTAGCATTTGCCGGACTGAAAACGCGCTACCCGCATATCGACACGCGCTCGCTGGGAAGGTCGGACGATTTGGAAGCCGCCATTGCGGCAGGTAGGACGATGGTTCGTATCGGCACGGCAAtttt                                                                                       >  2:849053/1‑149 (MQ=255)                                                                  tGAAGTTGCACGCCAAATGGCTGTTGCATTTGCCGGACTGAAAACGCGCTACCCGCATATCGACACGCTCTCTCTGGGAATGTCGGACGATATGGAATCCGCCATTGCGGTAGGTAGCACGATGGTTCGTATCGGCACTGCAATTTTTg                                                                                     <  1:71150/149‑1 (MQ=255)                                                                  tGAAGTTGCACGCCAAATGGCTGTAGCATTTGCCTGACTGAAAACGCGCTACCCGCATATCGACACGCTCTCTCTGGGAATGTCGGACGATATGGAAGCCGCCATTGCGGCAGGTAGCACGATGGTTCGTATCGGCACTGCAATTTTTg                                                                                     <  1:433965/149‑1 (MQ=255)                                                                   gAAGTTGCACGCCAAATGGCTGTAGCATTTGCCGGACTGAAAACGCGCTACCCGCATATCGACACGCTCTCTCTGGGAATGTCGGACGATATGGAAGCCGCCATTGCGGCAGGTAGCACGATGGTTCGTATCGGCAGTGCAATTTTTgg                                                                                    >  1:549417/1‑149 (MQ=255)                                                                   gAAGTTGCACACCAAATGGCTGTAGCATTTTCCGGACTGAAAACGCGCTACCCGCATATCGACACGCTCTCTCTGGGAATGTCGGACGTTATCGAAGCCTCCATTGCGGCAGGTAGCACGATGGTTCGTATCGGCACTGCAATTTTTgg                                                                                    >  2:919426/1‑149 (MQ=255)                                                                       ttGCACGCCAAATGGCTGTAGCATTTGCCGGACTGAAAACGCGCTACCCGCATATCGACACGCTCTCTCAGGGAATGTCGGACGATATGGAAGCCGGCATTGCGGCAGGTAGCACGAAGGTTCGTATCGGCACTGCAATTTTTGGTgcg                                                                                >  1:431476/1‑149 (MQ=255)                                                                       ttGCACGCCAAATGGCTGTAGCATTTGCCGGAATGAAAACGCGCTACCCGCATATCGACACGCTCTCTCTGGGATTGTCGGACGATATGGAAGCCGCCATTGCGGCAGGTAGCACGATGGTTCGTATCGGCACTGCAATTTTTGGTgcg                                                                                >  1:435439/1‑149 (MQ=255)                                                                                      cTGTAGCATTTGCCGGACTGAAAACGCGCTAGCCGCATATCGACACGCTCTGTCTGGGAATGTCGGACGATATGGAAGCCGCCATTGCGGCAGGTAGCACGATGGTTCGTATCGGCACTGCAATTTTTGGTGCGCGGGATTACTCTaaa                                                                 <  1:470444/149‑1 (MQ=255)                                                                                         tAGCATTTGCCGGACTGAAAACGCGCTACCCGCATATCGACACGCTCTCTCTGGGAATGTCGGACGATATGGAAGCCGCCATTGCGGCAGGTAGCACGATGGTTCGTATCGGCACTGCAATTTTTGGTGCGCGTGATTACTCTaaaaaa                                                              <  2:861719/149‑1 (MQ=255)                                                                                         aaGCATTTGCCGGACTGAAAACGCGCTACCCGCATATCGACACGCTCTCGCTGGGAATGTCGGACGATATGGAAGCCGCCATTGCGGCAGGTAGCACGATGGTTCGTATCGGCACTGCAATTTTTGGTGCCCGTGATTACTCTaaaaaa                                                              >  2:622620/2‑149 (MQ=255)                                                                                            cATTTGCCGGACTGAAAACGCGCTACCCGCATATCGCCACGCTCTCTCTGGGAATGTCGGACGATATGGAAGCCGCCATAGCGGCAGGTAGCACGAAGGTTCGTATCGGCACTGCTATTTTTGGTGCGCGTGATTACTCTAAAAAATaa                                                           >  1:631108/1‑149 (MQ=255)                                                                                            cATTTGCCGGACTGAAAACGCGCTACCCGCATATCGACACGCTCTCTCTGGGAATGTCGGACGATATGGAAGCCGCCATTGCGGCAGGTAGCACGATGGTTCGTATCGGCACTGCAATTTTTGGTGCGCGTGATTACTCTAAAAAATaa                                                           <  1:59344/149‑1 (MQ=255)                                                                                             aTTTGCCGGACTGAAAACGCGCTACCCGCATATCGACACGCTCTCTCTGGGAATGTCGGACGATATGGAAGCCGCCATTGCGGCAGGTAGCACGATGGTTCGTATCGGCACTGCAATTTTTGGTGCGCGTGATTACTCTAAAAAATAAg                                                          <  2:232364/149‑1 (MQ=255)                                                                                              tttGACGGACTGAAAACGCGCTACCCGCATATCGACACGCTCTCTCTGGGAATGCCGGACGATATGGAAGCCGCCATTGCGGCAGGTAGCACGATGGTTCGTATCGGCACTGCAATTTTTGGTGCGCGTGATTACTCTAAAAAATAAgg                                                         <  2:939126/149‑1 (MQ=255)                                                                                                tGCCGGACTGAAAACGCGCTACCCGCATATCGACACGCTCTCTCTGGGAATGTCGGACGATATGGAAGCCGCCATTGCGGCAGGTAGCACGATGGTTCGTATCGGCACTGCAATTTTTGGTGCGCGTGATTACTCTAAAAAATAAGGaa                                                       <  1:455046/149‑1 (MQ=255)                                                                                                  ccGGACTGAAAACGCGCTACCCGCATATCGACACGCTCTCTCTGGGAATGTCGGACGATATGGAAGCCGCCATTGCGGCAGGTAGCACGATGGTTCGTATCGGCACTGCAATTTTTGGTGCGCGTGATTACTCTAAAAAATAAGGAAtt                                                     <  2:396223/149‑1 (MQ=255)                                                                                                         gAAAACGCGCTACCCGCATATCGACACGCTCTCTCTGGGAATGTCGGACGATATGGAAGCCGCCATTGCGGCAGGTAGCACGATGGTTCGTATCGGCACTGCAATTTTTGGTGCGCGTCATTACTCTAAATAATAAGGAATTAAAGGaa                                              >  2:97185/1‑149 (MQ=255)                                                                                                          aaaaCGCGCTACCCGCATATCGACACGCTCTCTCTGGGAATGTCGGACGATATGGAAGCCGCCATTGCGGCAGGTAGCACGATGGTTCGTATCGGCACTGCAATTTTTGGTGCGCGTGATTACTCTAAAAAATAAGGAATTAAAGGAAc                                             >  1:299659/1‑149 (MQ=255)                                                                                                          aaaaCGCGCTACCCGCATATCGACACGCTCTCTCTGGGAATGTCGGACGATATGGAAGCCGCCATTGCGGCAGGTAGCACGATGGTTCGTATCGGCACTGCAATTTTTGGTGCGCGTGATTACGCTAAAAAATAAGGAATTAAAGGAAc                                             >  2:112708/1‑149 (MQ=255)                                                                                                          aaaaCGCGCTACCCGCATATCGACACGCTCTCTCTGGGAATGTCGGACGATATGGAAGCCGCCATTGCGGCAGGTAGCACGATGGTTCGTATCGGCACTGCAATTTTTGGTGCGCGTGATTACACTAAAAAATATGGAATTAAAGGAAc                                             >  1:329768/1‑149 (MQ=255)                                                                                                                 gcTACCCGCATATCGACACGCTCTCTCTGGGAATGTTGGACGATATGGAAGCCGCCATTGCGGCAGGTAGCACGATGGTTCGTATCGGCACTGCAATTTTTGGTGCGCGTGATTACTCTAAAAAATAAGGATTTAAAGGAACCACATGa                                      >  2:755292/1‑149 (MQ=255)                                                                                                                 gcTACCCGCATATCGACACGCTCTCTCTGGGAATGTTGGACGATATGGAAGCCGCCATTGCGGCAGGTAGCACGATGGTTAGTATCGGCACTGCAATTTTTGGTGCGCGTGATTACTCTAAAAAATAAGGAATTAAAGGAACGCCATGa                                      >  2:1030084/1‑149 (MQ=255)                                                                                                                  cTACCCGCATATCGACACGCTCTCTCTGGGAATGTCGGACGATATGGAAGCCGCCATTGCGGCAGGTAGCACGATGGTTCGTATCGGCACTGCAATTTTTGGTGCGCGTGATTACTCTAAAAAATAAGGAATTAAAGGAACGCCATGaa                                     <  2:963094/149‑1 (MQ=255)                                                                                                                    aCCCGCATTTCGACACGCTCTCTCTGGGAATGTCGGACGATATGGAAGCCGCCATTGCGGCAGGTAGCACGATGGTTCGTATCGGCACTGCAATTTTTGGTGCGCGTGATTACTCTAAAAAATAAGGAATTAAAGGAACGCCATGAATa                                   >  2:828721/1‑149 (MQ=255)                                                                                                                           tatCGACACGCTCTCTCTGGGAATGTCGGACGATATGGAAGCCGCCATTGCGGCAGGTAGCACGATGGTTCGTATCGGCACTGCAATTTTTGGTGCGCGTGATTACTCTAAAAAATAAGGAATTAAAGGAACGCCATGAATACGTTGAc                            <  1:637585/149‑1 (MQ=255)                                                                                                                                acacGCTCTCTCTGGGAATGTCGGACGATATGGAAGCCGCCATTGCGGCAGGTAGCACGATGGTACGTATCGGCACTGCAATTTTTGGTGCGCGTGATGACTCTAAAAAATAAGGAATTAAAGGAACGCCATGAATACGTTGACTTTcc                       >  2:215095/1‑149 (MQ=255)                                                                                                                                 caggCTCTCTGTGGGAATGTTGGACGATTTGGAAGCCGCCTTTGCGGCAGGTAGCACGATGGTTCGTATCGGCACTGCAATTTTTGGTGCGCGTGATTACTCTAAAAAATAAGGAATTAAAGGAACGCCATGAATACGTTGACTTTCCt                      <  1:755292/146‑1 (MQ=255)                                                                                                                                 cacGCTCTCTCTGGGAATGTTGGACGATATGGAAGCCGCCATTGCGGCAGGTAGCACGATGGTTCGTATCGGCACTGCAATTTTTGGTGCGCGTGATTACTCTAAAAAATAAGGAATTAAAGGAACGCCATGAATACGTTGACTTTCCt                      <  1:1030084/149‑1 (MQ=255)                                                                                                                                 cacGCGCTCTCTTGGAATGGCGGACGATATGGAAGCCGCCATTGCGGCAGGTAGCACGATGGTTCGTATCGGCACTGCAATTTATGGTGCGCGTGATTACTCTAAAAAATAAGGAATTAAAGGAACGCCATGAATACGTTGACTTTCCt                      <  2:459229/149‑1 (MQ=255)                                                                                                                                    gCTCTCTCTGGGAATGTCGGACGATATGGAAGCCGCCATTGCGGCAGGTAGCACGATGGTTCGTATCGGCACTGCAATTTTTGGTGCGCGTGATTACTCTAAAAAATAAGGAATTAAAGGAACGCCATGAATACGTTGACTTTCCTGCt                   >  2:798188/1‑149 (MQ=255)                                                                                                                                         ctctGGGAATGTCGGACGATATGGAAGCCGCCATTGCGGCAGGTAGCACGATGGTTCGTATCGGCACTGCAATTTTTGGTGCGCGTGATTACTCTAAAAAATAAGGAATTAAAGGAACGCCATGAATACGTTGACTTTCCTGCTTTCaa              >  1:275407/1‑149 (MQ=255)                                                                                                                                          tctGGGAATGTCGGACGATATGGAAGCCGCCATTGCGGCAGGTAGCACGATGGTTCGTATCGGCACTGCAATTTTTGGTGCGCGTGATTACTCTAAAAAATAAGGAATTAAAGGAACGCCATGAATACGTTGACTTTCCTGCTTTCAAc             <  1:867118/149‑1 (MQ=255)                                                                                                                                                 ctGTCGGACGATCTGGAAGCCGCCCTTGCGGCAGGTCGCACGATGGTTCGGATCGGCACTGCAATTTTTGGTGCGCGTGATTACTCTAAAAAATAAGGAATTAAAGGAACGCCATGAATACGTTGACTTTCCTGCTTTCAACGGTCAtt      <  2:485283/148‑1 (MQ=255)                                                                                                                                                 aTGTCGTACGATATTGAAGCCGCCATTGCGGCAGGTAGCACGATTGTTCGTATCGGCACTGCAATTTTTGGTGCGCGTGATTACTCTAAAAAATAAGGAATTAAAGGAACGCCATGAATACGTTGACTTTCCTGCTTTCAACgggcctt      <  1:654609/149‑6 (MQ=255)                                                                                                                                                   gTCGGACGATATGGAAGCCGCCATTGCGGCAGGTAGCACGATGGTTCGTATCGGCACTGCAATTTTTGGTGCGCGTGATTACTCTAAAAAATAAGGAATTAAAGGAACGCCATGAATACGTTGACTTTCCTGCTTTCAACGGTCATTGa    >  1:375307/1‑149 (MQ=255)                                                                                                                                                     cGGACGATATGGAAGCCGCCATTGCGGCAGGTAGCACGATGGTTCGTATCGGCACTGCAATTTTTGGTGCGCGTGATTACTCTAAAAAATAAGGAATTAAAGGAACGCCATGAATACGTTGACTTTCCTGCTTTCAACGGTCATTGAGc  <  1:949798/149‑1 (MQ=255)                                                                                                                                                     |                                                                                                                                                     CCGCGTTTACGTCTGCGCGGGTTGATGGCAATCCCTGCGCCTGAGTCAGAATATGTAAGGCAGTTTGAAGTTGCACGCCAAATGGCTGTAGCATTTGCCGGACTGAAAACGCGCTACCCGCATATCGACACGCTCTCTCTGGGAATGTCGGACGATATGGAAGCCGCCATTGCGGCAGGTAGCACGATGGTTCGTATCGGCACTGCAATTTTTGGTGCGCGTGATTACTCTAAAAAATAAGGAATTAAAGGAACGCCATGAATACGTTGACTTTCCTGCTTTCAACGGTCATTGAGC  >  NC_000913/3095563‑3095859

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑