Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,256,441 T→G 8.8% T71P (ACG→CCG)  yhaM ← putative L‑serine dehydratase alpha chain

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,256,4410TG 8.8% 141.1 / 2.6 68T71P (ACG→CCG) yhaMputative L‑serine dehydratase alpha chain
Reads supporting (aligned to +/- strand):  ref base T (28/33);  new base G (3/4);  total (31/37)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.51e-01

ATCTTAACGGAGACTTTCCCCGCCGCCAGCAGTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCGCAGCAACCGCCGCCGCCAGCGCCAGTGAAATCGGTTCAGTACATCCCAGCGCCGGTTT  >  NC_000913/3256293‑3256588
                                                                                                                                                    |                                                                                                                                                   
aTCTTAACGGAGACTTTCCCCGCAGCCAGCAGTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCGTTCAGCTCTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCCGCCCCCCCATTCCCGt                                                                                                                                                     >  1:671293/1‑149 (MQ=255)
     aaCGGAGACTTTCCCCGCCGCCAGCAGTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTTACGCCCCCAGCGCCGCCGCAATCGGCCGCCCCACCATTCCCGTGCCgg                                                                                                                                                >  1:335475/1‑149 (MQ=255)
     aaCGGAGACTTTCCCCGCCGCCAGCAGTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCgg                                                                                                                                                <  1:737193/149‑1 (MQ=255)
     aaCGGAGACTTTCCCCGCCGCCAGCAGAGCTTAGGCATCGTCAATTGCCAGCGCTGTTGCGACTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCCCCGCCGCACTCGGCAGCCCCACCATACCCGTGCCgg                                                                                                                                                >  1:415985/1‑149 (MQ=255)
     aaCGGAGACTTTCCCCGCCGCCAGCAGAGCTTAGGCATCGGCAATACCAAGCGCTGAGGCTTCGATCAGGGCTGCCAGCCCGGCGTTGGCATTTCAACATACCGCCCCCAGCGCCGCCGGAATCGGCAGCCCCACCCTTCGCGGGGCgg                                                                                                                                                >  2:274957/1‑149 (MQ=255)
        ggAGACTTTCCCCGCCGCCAGCAGTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGaa                                                                                                                                             >  1:102583/1‑149 (MQ=255)
         gagaCTTTCCCCGCCGCCAGCAGTGCTTTGGCATCGGCAATTGCCTGCGCTGTGGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAAc                                                                                                                                            <  2:144367/149‑1 (MQ=255)
          agaCTTTCCCCGCCGCCAGCAGTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACg                                                                                                                                           >  1:7050/1‑149 (MQ=255)
            ccTTTCCCCGCCGCCAGCAGTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGt                                                                                                                                         <  1:878143/148‑1 (MQ=255)
               ttCCCCGCCGCCAGCAGTGCTTTGTCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCGTTCCCGGCACGGGAATGGTggg                                                                                                                                      <  2:643940/149‑3 (MQ=255)
               ttCCCCGCCGCCAGCAGTGCTTTGGCAGCGGCAATTGCCTGCGCTGGTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTACCGCCCCCAGCGCCGCCGCTATCGTCAGCCCCACCGTTCCCGGCACGGGAATGGTggg                                                                                                                                      <  1:69698/149‑3 (MQ=255)
                   ccgccgCCAGCAGTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGACGGGAACGGTGACGccc                                                                                                                                  >  2:392736/1‑149 (MQ=255)
                    cgccgcCAGCACAGCTTTGGCATCGGCAATTGAAACCGCTGTTGAGTCTATCAGAACTTACAGCCCTGAGTTGGCATTTCGACATAACGCACCCAGCGCCGCCGAAATCGGCAGCCCCACCATTCCCGGGCCGGGAACGGGGACGCCCa                                                                                                                                 >  2:430494/1‑149 (MQ=255)
                       cgccAGCAGTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGAc                                                                                                                              <  1:492914/149‑1 (MQ=255)
                        gccAGCAGTGCTTTGGCATCGGCAATTGACTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCTGCTTTGGCATTTCCACCTAACGCCCACAGCGACGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGAAGCCAAGAca                                                                                                                             >  2:734789/1‑148 (MQ=255)
                          cagcagTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGt                                                                                                                           <  2:574030/149‑1 (MQ=255)
                              agTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGGCACGGGAATGGTggg                                                                                                                                      <  1:963736/134‑3 (MQ=255)
                              agTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGGCACGGGAATGGTggg                                                                                                                                      >  2:963736/1‑132 (MQ=255)
                               gTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGttcttc                                                                                                                      <  2:102583/149‑1 (MQ=255)
                                 gCTTTGGCATCGGCAATTGCCTGCGCGGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTCGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCAGGGAACGGTGACGCCCAGACCGTTCTtcat                                                                                                                    <  2:181670/149‑1 (MQ=255)
                                   tttGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTtcatca                                                                                                                  <  2:15754/149‑1 (MQ=255)
                                     tGGCAGCGGCAATTGCCTGCGCTGTTGCGTCGTGCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGACCAGACCGTTCTTCATCAGa                                                                                                                <  1:686911/149‑1 (MQ=255)
                                         aTCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGACCGGTGACGCCCAGACCGGTGTTCATCAGCTTTg                                                                                                            >  2:800894/1‑149 (MQ=255)
                                          tCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTgg                                                                                                           >  1:8964/1‑149 (MQ=255)
                                           cGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGc                                                                                                          <  2:97441/149‑1 (MQ=255)
                                            ggCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCg                                                                                                         >  2:389704/1‑149 (MQ=255)
                                             gCAATTGCCTGCGCTGTTTCGTCTTGCAGAACTTCCAGCCCGGCGTTGGCATTTCCACCTCACGCCCCCAGCGCCGGCGCAATCGGCCGCCCCACCATTCCCGTGCCGGGAACGGAGCCGCCCAGCCCGTGCGTCAGAAGAATTggggc                                                                                                        >  2:996467/1‑146 (MQ=255)
                                                   gCCTGCGCTGTTTCGTCTTTCCGCACATCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACAGTTCTTCATCAGATTTGGCGAAACCCa                                                                                                  <  2:756529/149‑1 (MQ=255)
                                                    ccTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAg                                                                                                 >  2:251584/1‑149 (MQ=255)
                                                       gcgcTGTTGCGTCTTTCAGCACTTCCCGCCCGGCGTTGGCATTTCCACCTAAAGCCCCCAGCGCCGCCGCAAACGGCAGCCCCACCATTACCGTCCCGGGCACTGTGCCGCCCAGACCGTTCTACATCAGATTTGCCGCAACCCCGGCt                                                                                              >  1:511987/1‑149 (MQ=255)
                                                        cgcttgtGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCATACCGTTCTTCATCAGATTTGGCGAAACCCAGGCtt                                                                                             <  1:234530/143‑1 (MQ=255)
                                                        cgcTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCTCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCtt                                                                                             <  2:900087/149‑1 (MQ=255)
                                                           tGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCATCATCAGATTTGGCGAAACCCAGGCTTCTa                                                                                          <  2:1022516/149‑1 (MQ=255)
                                                            gTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTac                                                                                         <  2:162473/149‑1 (MQ=255)
                                                               gCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGGGACGCCCAGCCCGTTCTTCATCAGAGTTGGCGAAACCCAGGCTTCTACACg                                                                                      >  2:771398/1‑149 (MQ=255)
                                                                 ggcTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGtt                                                                                    <  2:524773/147‑1 (MQ=255)
                                                                 gTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTGCTCCACGta                                                                                    >  1:827960/1‑148 (MQ=255)
                                                                   cTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGCCCGTTCGGCAGCAGATTTGGCGAAACCCAGGCTTCTACACGTTCa                                                                                  >  1:353260/1‑149 (MQ=255)
                                                                   cTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACAATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTGAATCAGATTTGGCGACACCCCGGCGTAGGCACGTTcc                                                                                  >  1:1004641/1‑148 (MQ=255)
                                                                        aGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCgg                                                                             >  1:388517/1‑149 (MQ=255)
                                                                         gCACTTCCGGCCCGGCGTTGGCATTTCCACCTAACGCCCCCCGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCCGGCGTCGACACGTTCAACCGGa                                                                            >  2:192609/1‑149 (MQ=255)
                                                                         gCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGa                                                                            <  1:175831/149‑1 (MQ=255)
                                                                         gCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCAACATTCCCGTGCCGGGAACGGTGACGCCCAGAGCGTTCTTCAGCAGATTTGGCGAAACCCAGGCGTCTACACGTTCAACCGGa                                                                            >  2:290211/1‑149 (MQ=255)
                                                                          cACTTCCAGCCCGGCGTTGGCATTTCCCCCTAACGCCCCCAGCGCCGCCGCACTCGGCAGCCCCACCATTCCCGTGCCGGGCTCGGTGACGCCCAGACCGTTCTTCAGCAGATTTGGGGAAACCCAGGCGTCTACACGTTCAACCGGAc                                                                           >  1:160131/1‑149 (MQ=255)
                                                                             ttCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGCACGGTGCCGCCCAGCCCGTTGGTCGTCAGATTTGGCGGAACCCAGGCTTGTACACGTTCGTCCGGACCtt                                                                        >  2:882492/1‑149 (MQ=255)
                                                                              tCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTc                                                                       <  1:712936/149‑1 (MQ=255)
                                                                              tCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTc                                                                       <  1:644062/149‑1 (MQ=255)
                                                                                     cGGCGTTGGCATTTCCAACTCACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCt                                                                <  2:193871/149‑1 (MQ=255)
                                                                                       gCGTAGTCATTTCCACATAACGCGCCCAGCGCATCCGCAATCGGCAGCCCCACCATTCCCGTGCCTGGAACGGTGACGCCAAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGc                                                              <  1:915676/149‑1 (MQ=255)
                                                                                          ttGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGCCCTTCCAGTTCTGCCgc                                                           >  2:142019/1‑149 (MQ=255)
                                                                                                     aCCTAACGCCCCCAGCGCCGCCGGAATCGGCAGCCCCACCATTCCCGTGCCGGGCACGGTGACGCCCAGACCGGTCTTCATCAGATTTGGCGACACCCCGGCTTGTACACGTTCAACCGGCCCTTGGAGATCTGCCGCAGCACccgccg                                                >  1:533522/1‑149 (MQ=255)
                                                                                                       cTAACGCCCCCAGCGCCGCCGCCATCGGCAGCCCCACCCTTCCCGTGCCGGGAACGGTGACGCCCAGACCGTGCTTCATCAGCTTTGGCGAAACCCAGGCTTCTCCACGTTCAACCGGACCTTCCAGTTCTGCCGCAGCAAccgccgcc                                              <  1:173940/149‑1 (MQ=255)
                                                                                                                   ccgccgccgcCATCGGCATCCCCACCATTCACGTTCCTGTAAAGGAGACGCCCTGCCCGTTCTTCATCTGATTTGGCTAACCCCATGCTTCTACTCGTTCATCCGGACCTTCCAGTTCTGCCGCAGCATCCGCCGCCGCCAGCGCCAGt                                  <  2:671293/148‑1 (MQ=255)
                                                                                                                    cgccgccgcAATCGGCAGCCCCACCATTCCCTTGCCGGGAACGGTGACGCACAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTTCACGTTCTACCGGACCTTCCAGTTCTGCCGCAGCAACCGCCGCCGCCAGCGCCAGTg                                 <  2:702255/149‑1 (MQ=255)
                                                                                                                       cgccgcAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCACCCGGACCTTCCAGTTCTGCCGCAGCAACCGCCGCCGCCAGCGCCAGTGaaa                              >  1:742752/1‑149 (MQ=255)
                                                                                                                          cgcAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCGCAGCAACCGCCGCCGCCAGCGCCAGTGAAATCg                           <  2:866078/149‑1 (MQ=255)
                                                                                                                             aaTCGGCAGCCCCACCATTCCCGTGCCGGGAACTGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCGCAGCAACCGCCGCCGCCAGCGCCAGTGAAATCGGtt                        <  2:335475/149‑1 (MQ=255)
                                                                                                                             aaTCGGCAGCCCCACCAATCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCGCAGCAACCGCCGCCGCCAGCGCCAGTGAAATCGGtt                        <  2:291512/149‑1 (MQ=255)
                                                                                                                                 tgCAGCCTAACCATTCCCTTTGCGGGAACGGTGCCGCCCAGACCGTTCTTCATCAGATATGGCGAAACGCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTATGCGGCAGCAACCGCCGCCGCCAGCGCCAGTGAAATCGGTTCAGt                    <  2:24996/148‑1 (MQ=255)
                                                                                                                                 ggCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCGCAGCAACCGCCGCCGCCAGCGCCAGTGAAATCGGTTCAGt                    <  2:853525/149‑1 (MQ=255)
                                                                                                                                      ccgcaccaTTGCCGTGCCGGGAACGGTGACGCACAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCTACCGGACCTTCCAGTTCTGCCGCAGCAAACGCCGCCGCCAGCGCCAGTGAAATCGGTTCAGTACATc               <  1:734789/146‑1 (MQ=255)
                                                                                                                                       cccACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCTTCAGATTTGGCGAAACCCAGGCTTCACCACGTTCAACCGGACCTTCCAGATCAGCCGACGCAACCGCCGCCGCCAGCGCCAGTGAAATCGGTTCAGTACATcc              <  1:274957/149‑1 (MQ=255)
                                                                                                                                       cccACCATTCCCGGGCCGGGAACGGGGACGCCCAGACCGTTCTTCATCAGAGTTGGCGAACCCCAGGCTGCTACACGTTCAACCGGACCTTACAGTTCTGCCGCAGCAACCGCCGCCGCCAGCGCCAGTGAAATCGGTTCAGTACATcc              <  1:430494/149‑1 (MQ=255)
                                                                                                                                         caccaTTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCGCAGCAACCGCCGCCGCCAGCGCCAGTGAAATCGGTTCAGTACATcccc            >  1:310992/1‑148 (MQ=255)
                                                                                                                                            caTTCCCGTGCCGGGAACGGTGACGCCCAGCCCGTTCTTCATCAGATTTGGCGAAACCCAGGCGTCTACCCGTTCACCCGGACCTTCCAGTTCTGCCGCAGCAACCGCCGCCGCCAGCGCCAGTGAACTCGGTTCAGTCCATCCCcgcg         >  1:756730/1‑149 (MQ=255)
                                                                                                                                                 ccGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAACCCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCGCAGCAACCGCCGCCGCCAGCGCCAGTGACATAGGTTCAGTCCATCCCCGCGCCGGt    >  2:802840/1‑149 (MQ=255)
                                                                                                                                                 ccGTGCCGGGAACGGAGAGGCCCAGACCCTTCTTCATCAGATTTGGCGAAACCCCGGCTTCTACACTTTCAACCGGACCTTCCAGTTCTGCCGCAGCAACCGCCGCCGCCAGCGCCAGTGAAATCGGTTCTGTACATCCCAGCGCCGGt    <  2:806178/149‑1 (MQ=255)
                                                                                                                                                  cGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCGCAGCAACCGCCGCCGCCAGCGCCAGTGAAATCGGTTCAGTACATCCCAGAGCCGGtt   >  2:61646/1‑149 (MQ=255)
                                                                                                                                                   gTGCCGGGAACTGTGACGCCCAGACCGTTCTTCATCAGTTTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGGTCTTCCGCAGCAACCGCCGCCGCCAGCGCCAGTGAAATCGGTTCAGTACATCCCAGCGCCGGttt  <  2:133970/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
ATCTTAACGGAGACTTTCCCCGCCGCCAGCAGTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCGCAGCAACCGCCGCCGCCAGCGCCAGTGAAATCGGTTCAGTACATCCCAGCGCCGGTTT  >  NC_000913/3256293‑3256588

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: