Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,256,442 G→C 7.0% G70G (GGC→GGG yhaM ← putative L‑serine dehydratase alpha chain

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,256,4420GC 7.0% 167.5 / 4.3 68G70G (GGC→GGGyhaMputative L‑serine dehydratase alpha chain
Reads supporting (aligned to +/- strand):  ref base G (29/32);  new base C (2/3);  total (31/37)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.65e-01

AACGGAGACTTTCCCCGCCGCCAGCAGTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCGCAGCAACCGCCGCCGCCAGCGCCAGTGAAATCGGTTCAGTACATCCCAGCGCCGGTTTTA  >  NC_000913/3256298‑3256590
                                                                                                                                                |                                                                                                                                                    
aaCGGAGACTTTCCCCGCCGCCAGCAGTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTTACGCCCCCAGCGCCGCCGCAATCGGCCGCCCCACCATTCCCGTGCCgg                                                                                                                                                  >  1:335475/1‑149 (MQ=255)
aaCGGAGACTTTCCCCGCCGCCAGCAGTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCgg                                                                                                                                                  <  1:737193/149‑1 (MQ=255)
aaCGGAGACTTTCCCCGCCGCCAGCAGAGCTTAGGCATCGTCAATTGCCAGCGCTGTTGCGACTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCCCCGCCGCACTCGGCAGCCCCACCATACCCGTGCCgg                                                                                                                                                  >  1:415985/1‑149 (MQ=255)
aaCGGAGACTTTCCCCGCCGCCAGCAGAGCTTAGGCATCGGCAATACCAAGCGCTGAGGCTTCGATCAGGGCTGCCAGCCCGGCGTTGGCATTTCAACATACCGCCCCCAGCGCCGCCGGAATCGGCAGCCCCACCCTTCGCGGGGCgg                                                                                                                                                  >  2:274957/1‑149 (MQ=255)
   ggAGACTTTCCCCGCCGCCAGCAGTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGaa                                                                                                                                               >  1:102583/1‑149 (MQ=255)
    gagaCTTTCCCCGCCGCCAGCAGTGCTTTGGCATCGGCAATTGCCTGCGCTGTGGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAAc                                                                                                                                              <  2:144367/149‑1 (MQ=255)
     agaCTTTCCCCGCCGCCAGCAGTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACg                                                                                                                                             >  1:7050/1‑149 (MQ=255)
       ccTTTCCCCGCCGCCAGCAGTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGt                                                                                                                                           <  1:878143/148‑1 (MQ=255)
          ttCCCCGCCGCCAGCAGTGCTTTGTCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCGTTCCCGGCACGGGAATGGTggg                                                                                                                                        <  2:643940/149‑3 (MQ=255)
          ttCCCCGCCGCCAGCAGTGCTTTGGCAGCGGCAATTGCCTGCGCTGGTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTACCGCCCCCAGCGCCGCCGCTATCGTCAGCCCCACCGTTCCCGGCACGGGAATGGTggg                                                                                                                                        <  1:69698/149‑3 (MQ=255)
              ccgccgCCAGCAGTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGACGGGAACGGTGACGccc                                                                                                                                    >  2:392736/1‑149 (MQ=255)
               cgccgcCAGCACAGCTTTGGCATCGGCAATTGAAACCGCTGTTGAGTCTATCAGAACTTACAGCCCTGAGTTGGCATTTCGACATAACGCACCCAGCGCCGCCGAAATCGGCAGCCCCACCATTCCCGGGCCGGGAACGGGGACGCCCa                                                                                                                                   >  2:430494/1‑149 (MQ=255)
                  cgccAGCAGTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGAc                                                                                                                                <  1:492914/149‑1 (MQ=255)
                   gccAGCAGTGCTTTGGCATCGGCAATTGACTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCTGCTTTGGCATTTCCACCTAACGCCCACAGCGACGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGAAGCCAAGAca                                                                                                                               >  2:734789/1‑148 (MQ=255)
                     cagcagTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGt                                                                                                                             <  2:574030/149‑1 (MQ=255)
                         agTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGGCACGGGAATGGTggg                                                                                                                                        <  1:963736/134‑3 (MQ=255)
                         agTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGGCACGGGAATGGTggg                                                                                                                                        >  2:963736/1‑132 (MQ=255)
                          gTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGttcttc                                                                                                                        <  2:102583/149‑1 (MQ=255)
                            gCTTTGGCATCGGCAATTGCCTGCGCGGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTCGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCAGGGAACGGTGACGCCCAGACCGTTCTtcat                                                                                                                      <  2:181670/149‑1 (MQ=255)
                              tttGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTtcatca                                                                                                                    <  2:15754/149‑1 (MQ=255)
                                tGGCAGCGGCAATTGCCTGCGCTGTTGCGTCGTGCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGACCAGACCGTTCTTCATCAGa                                                                                                                  <  1:686911/149‑1 (MQ=255)
                                    aTCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGACCGGTGACGCCCAGACCGGTGTTCATCAGCTTTg                                                                                                              >  2:800894/1‑149 (MQ=255)
                                     tCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTgg                                                                                                             >  1:8964/1‑149 (MQ=255)
                                      cGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGc                                                                                                            <  2:97441/149‑1 (MQ=255)
                                       ggCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCg                                                                                                           >  2:389704/1‑149 (MQ=255)
                                        gCAATTGCCTGCGCTGTTTCGTCTTGCAGAACTTCCAGCCCGGCGTTGGCATTTCCACCTCACGCCCCCAGCGCCGGCGCAATCGGCCGCCCCACCATTCCCGTGCCGGGAACGGAGCCGCCCAGCCCGTGCGTCAGAAGAATTggggc                                                                                                          >  2:996467/1‑146 (MQ=255)
                                              gCCTGCGCTGTTTCGTCTTTCCGCACATCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACAGTTCTTCATCAGATTTGGCGAAACCCa                                                                                                    <  2:756529/149‑1 (MQ=255)
                                               ccTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAg                                                                                                   >  2:251584/1‑149 (MQ=255)
                                                  gcgcTGTTGCGTCTTTCAGCACTTCCCGCCCGGCGTTGGCATTTCCACCTAAAGCCCCCAGCGCCGCCGCAAACGGCAGCCCCACCATTACCGTCCCGGGCACTGTGCCGCCCAGACCGTTCTACATCAGATTTGCCGCAACCCCGGCt                                                                                                >  1:511987/1‑149 (MQ=255)
                                                   cgcttgtGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCATACCGTTCTTCATCAGATTTGGCGAAACCCAGGCtt                                                                                               <  1:234530/143‑1 (MQ=255)
                                                   cgcTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCTCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCtt                                                                                               <  2:900087/149‑1 (MQ=255)
                                                      tGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCATCATCAGATTTGGCGAAACCCAGGCTTCTa                                                                                            <  2:1022516/149‑1 (MQ=255)
                                                       gTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTac                                                                                           <  2:162473/149‑1 (MQ=255)
                                                          gCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGGGACGCCCAGCCCGTTCTTCATCAGAGTTGGCGAAACCCAGGCTTCTACACg                                                                                        >  2:771398/1‑149 (MQ=255)
                                                            ggcTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGtt                                                                                      <  2:524773/147‑1 (MQ=255)
                                                            gTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTGCTCCACGta                                                                                      >  1:827960/1‑148 (MQ=255)
                                                              cTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGCCCGTTCGGCAGCAGATTTGGCGAAACCCAGGCTTCTACACGTTCa                                                                                    >  1:353260/1‑149 (MQ=255)
                                                              cTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACAATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTGAATCAGATTTGGCGACACCCCGGCGTAGGCACGTTcc                                                                                    >  1:1004641/1‑148 (MQ=255)
                                                                   aGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCgg                                                                               >  1:388517/1‑149 (MQ=255)
                                                                    gCACTTCCGGCCCGGCGTTGGCATTTCCACCTAACGCCCCCCGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCCGGCGTCGACACGTTCAACCGGa                                                                              >  2:192609/1‑149 (MQ=255)
                                                                    gCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGa                                                                              <  1:175831/149‑1 (MQ=255)
                                                                    gCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCAACATTCCCGTGCCGGGAACGGTGACGCCCAGAGCGTTCTTCAGCAGATTTGGCGAAACCCAGGCGTCTACACGTTCAACCGGa                                                                              >  2:290211/1‑149 (MQ=255)
                                                                     cACTTCCAGCCCGGCGTTGGCATTTCCCCCTAACGCCCCCAGCGCCGCCGCACTCGGCAGCCCCACCATTCCCGTGCCGGGCTCGGTGACGCCCAGACCGTTCTTCAGCAGATTTGGGGAAACCCAGGCGTCTACACGTTCAACCGGAc                                                                             >  1:160131/1‑149 (MQ=255)
                                                                        ttCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGCACGGTGCCGCCCAGCCCGTTGGTCGTCAGATTTGGCGGAACCCAGGCTTGTACACGTTCGTCCGGACCtt                                                                          >  2:882492/1‑149 (MQ=255)
                                                                         tCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTc                                                                         <  1:712936/149‑1 (MQ=255)
                                                                         tCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTc                                                                         <  1:644062/149‑1 (MQ=255)
                                                                                cGGCGTTGGCATTTCCAACTCACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCt                                                                  <  2:193871/149‑1 (MQ=255)
                                                                                  gCGTAGTCATTTCCACATAACGCGCCCAGCGCATCCGCAATCGGCAGCCCCACCATTCCCGTGCCTGGAACGGTGACGCCAAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGc                                                                <  1:915676/149‑1 (MQ=255)
                                                                                     ttGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGCCCTTCCAGTTCTGCCgc                                                             >  2:142019/1‑149 (MQ=255)
                                                                                                aCCTAACGCCCCCAGCGCCGCCGGAATCGGCAGCCCCACCATTCCCGTGCCGGGCACGGTGACGCCCAGACCGGTCTTCATCAGATTTGGCGACACCCCGGCTTGTACACGTTCAACCGGCCCTTGGAGATCTGCCGCAGCACccgccg                                                  >  1:533522/1‑149 (MQ=255)
                                                                                                  cTAACGCCCCCAGCGCCGCCGCCATCGGCAGCCCCACCCTTCCCGTGCCGGGAACGGTGACGCCCAGACCGTGCTTCATCAGCTTTGGCGAAACCCAGGCTTCTCCACGTTCAACCGGACCTTCCAGTTCTGCCGCAGCAAccgccgcc                                                <  1:173940/149‑1 (MQ=255)
                                                                                                              ccgccgccgcCATCGGCATCCCCACCATTCACGTTCCTGTAAAGGAGACGCCCTGCCCGTTCTTCATCTGATTTGGCTAACCCCATGCTTCTACTCGTTCATCCGGACCTTCCAGTTCTGCCGCAGCATCCGCCGCCGCCAGCGCCAGt                                    <  2:671293/148‑1 (MQ=255)
                                                                                                               cgccgccgcAATCGGCAGCCCCACCATTCCCTTGCCGGGAACGGTGACGCACAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTTCACGTTCTACCGGACCTTCCAGTTCTGCCGCAGCAACCGCCGCCGCCAGCGCCAGTg                                   <  2:702255/149‑1 (MQ=255)
                                                                                                                  cgccgcAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCACCCGGACCTTCCAGTTCTGCCGCAGCAACCGCCGCCGCCAGCGCCAGTGaaa                                >  1:742752/1‑149 (MQ=255)
                                                                                                                     cgcAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCGCAGCAACCGCCGCCGCCAGCGCCAGTGAAATCg                             <  2:866078/149‑1 (MQ=255)
                                                                                                                        aaTCGGCAGCCCCACCATTCCCGTGCCGGGAACTGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCGCAGCAACCGCCGCCGCCAGCGCCAGTGAAATCGGtt                          <  2:335475/149‑1 (MQ=255)
                                                                                                                        aaTCGGCAGCCCCACCAATCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCGCAGCAACCGCCGCCGCCAGCGCCAGTGAAATCGGtt                          <  2:291512/149‑1 (MQ=255)
                                                                                                                            tgCAGCCTAACCATTCCCTTTGCGGGAACGGTGCCGCCCAGACCGTTCTTCATCAGATATGGCGAAACGCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTATGCGGCAGCAACCGCCGCCGCCAGCGCCAGTGAAATCGGTTCAGt                      <  2:24996/148‑1 (MQ=255)
                                                                                                                            ggCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCGCAGCAACCGCCGCCGCCAGCGCCAGTGAAATCGGTTCAGt                      <  2:853525/149‑1 (MQ=255)
                                                                                                                                 ccgcaccaTTGCCGTGCCGGGAACGGTGACGCACAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCTACCGGACCTTCCAGTTCTGCCGCAGCAAACGCCGCCGCCAGCGCCAGTGAAATCGGTTCAGTACATc                 <  1:734789/146‑1 (MQ=255)
                                                                                                                                  cccACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCTTCAGATTTGGCGAAACCCAGGCTTCACCACGTTCAACCGGACCTTCCAGATCAGCCGACGCAACCGCCGCCGCCAGCGCCAGTGAAATCGGTTCAGTACATcc                <  1:274957/149‑1 (MQ=255)
                                                                                                                                  cccACCATTCCCGGGCCGGGAACGGGGACGCCCAGACCGTTCTTCATCAGAGTTGGCGAACCCCAGGCTGCTACACGTTCAACCGGACCTTACAGTTCTGCCGCAGCAACCGCCGCCGCCAGCGCCAGTGAAATCGGTTCAGTACATcc                <  1:430494/149‑1 (MQ=255)
                                                                                                                                    caccaTTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCGCAGCAACCGCCGCCGCCAGCGCCAGTGAAATCGGTTCAGTACATcccc              >  1:310992/1‑148 (MQ=255)
                                                                                                                                       caTTCCCGTGCCGGGAACGGTGACGCCCAGCCCGTTCTTCATCAGATTTGGCGAAACCCAGGCGTCTACCCGTTCACCCGGACCTTCCAGTTCTGCCGCAGCAACCGCCGCCGCCAGCGCCAGTGAACTCGGTTCAGTCCATCCCcgcg           >  1:756730/1‑149 (MQ=255)
                                                                                                                                            ccGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAACCCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCGCAGCAACCGCCGCCGCCAGCGCCAGTGACATAGGTTCAGTCCATCCCCGCGCCGGt      >  2:802840/1‑149 (MQ=255)
                                                                                                                                            ccGTGCCGGGAACGGAGAGGCCCAGACCCTTCTTCATCAGATTTGGCGAAACCCCGGCTTCTACACTTTCAACCGGACCTTCCAGTTCTGCCGCAGCAACCGCCGCCGCCAGCGCCAGTGAAATCGGTTCTGTACATCCCAGCGCCGGt      <  2:806178/149‑1 (MQ=255)
                                                                                                                                             cGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCGCAGCAACCGCCGCCGCCAGCGCCAGTGAAATCGGTTCAGTACATCCCAGAGCCGGtt     >  2:61646/1‑149 (MQ=255)
                                                                                                                                              gTGCCGGGAACTGTGACGCCCAGACCGTTCTTCATCAGTTTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGGTCTTCCGCAGCAACCGCCGCCGCCAGCGCCAGTGAAATCGGTTCAGTACATCCCAGCGCCGGttt    <  2:133970/149‑1 (MQ=255)
                                                                                                                                                gCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCGCAGCAACCGCCGCCGCCAGCGCCAGTGAAATCGGTTCAGTACATCCCAGCGCCGGTTTTa  <  1:800894/149‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                                    
AACGGAGACTTTCCCCGCCGCCAGCAGTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCGCAGCAACCGCCGCCGCCAGCGCCAGTGAAATCGGTTCAGTACATCCCAGCGCCGGTTTTA  >  NC_000913/3256298‑3256590

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: