Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,269,956 T→C 20.8% intergenic (+354/+260) yhaC → / ← rnpB pentapetide repeats‑related protein/RNase P, M1 RNA component

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,269,9560TC20.8% 49.9 / 13.5 39intergenic (+354/+260)yhaC/rnpBpentapetide repeats‑related protein/RNase P, M1 RNA component
Reads supporting (aligned to +/- strand):  ref base T (5/23);  new base C (3/4);  total (12/27)
Fisher's exact test for biased strand distribution p-value = 3.12e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.14e-01

CATCCGGCATGAATAAAGCGCACTCAACAATCTGAAACCCGCCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTAC  >  NC_000913/3269812‑3270095
                                                                                                                                                |                                                                                                                                           
cATCCGGCATGAATAAAGCGCACTCAACAATCTGAAACCCGCCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGAAGCTTTCTTGAGCCTTTTGAGACGTGGACAGTAATTCATCTTTATGGCCCTCAAAAGGTaaaa                                                                                                                                         >  1:9583/1‑149 (MQ=255)
      gCATGAATAAAGCGCACTCAACAAACTGAAACCCGCCGGAACGGTTTATTACGTACCTCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGAAAAAAccacc                                                                                                                                   >  1:589079/1‑146 (MQ=255)
          gAATAAAGCGCACTCAACAATCTGAAACCCGCCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTAATAGTTCTGCGCCGCCAAAAGTAAAAACccgactaa                                                                                                                               >  1:552391/1‑144 (MQ=21)
             ttaatcccACTCAAAAATCTAAAACCCGCAGTATCGGTTTATTACGTACAACCGGTTAAACTGACCGATAAGACGATTTCTTTTGTTTATAGCGTCGTTGACAGTGATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCg                                                                                                                            <  2:352647/142‑1 (MQ=11)
              aaaGCGCACTCTACAATCTGAAACCCGCCGGAACGGTTTATTAGGTACATCAGGTAAAACTGACCGATAAGACGCTTTCTTTTGGGTATATTGTCGCGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTTAAAACCCGCCGAAGCgg                                                                                                                           <  1:933663/149‑1 (MQ=18)
                 tcgcACTCAACAATCTGAAACCCGCCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGGCGTGGACAGTCATGCATCTTTCTGCCCCTCCAAACGTAAAAACCCGCCGAAGCGGGtt                                                                                                                        <  2:487897/148‑1 (MQ=255)
                 gcgcACTCAACAATCTGAAACCCGCCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGACATTCATCTTTCTGCCCCTCCAAAAGAAAAAACCCGCCGAAGCGGGtt                                                                                                                        >  1:400667/1‑149 (MQ=255)
                  cgcACTCAACAATCTGAAACCCGCCGGAACTGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGttt                                                                                                                       <  1:443620/149‑1 (MQ=255)
                    cACTCAACACTCTGCATACCGCCGGTAGGTTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGCGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCTTCGGCGGGttttt                                                                                                                     <  2:1025190/149‑1 (MQ=17)
                    cACTCAACAATCTGAAACCCGCCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTAATTAATCGTTCTGCGCCTCCAAAAGAAAAAACCCGCCGAAGCGGGgtttt                                                                                                                     >  2:117710/1‑149 (MQ=18)
                    cACTCAACAAGCTGAACCCCGCCGGAACGGTTTATAACGTACATCACGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGGGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGttttt                                                                                                                     <  1:461201/149‑1 (MQ=18)
                      cTCAACAATCTGAAACCCGCCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTAc                                                                                                                   <  1:362538/149‑1 (MQ=255)
                      cTCAACAACCTGAAACCCGCCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTAc                                                                                                                   <  1:93690/149‑1 (MQ=255)
                           caaTCTGAAACCCGCCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCTATTAGCAGCTTACTATTGGTTATATTGTCTTTTACAGTCATTAATCTTACTTCCCCTCCAAAAGAAAAAAAAAAAAAAAGCGGGTTTTTTCGTaaa                                                                                                              >  2:14467/1‑149 (MQ=255)
                            aaTCTGAAACCCGCCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTaaaa                                                                                                             <  1:19244/149‑1 (MQ=255)
                            aaTCTGAAACCCGCCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGAAAAGTGACGAGCAACGTCAATCCAATGACTGAACCCGCAACAATAAAAACCAGCACAAGCGCGTATTTACGTaaaa                                                                                                             >  2:351502/1‑149 (MQ=12)
                               tttttaCCAGCCGTAACGGTTTATTACGTACATCAGGTTATAGTGACGGTTTTGCCGCTTTCTTTTGGGTATAGTGTCGTTGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCAGCCGAAGCGGGTTTTTACGTAAAACAg                                                                                                          <  2:584242/144‑1 (MQ=14)
                                 gAAACCCGCCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGt                                                                                                        <  1:545252/149‑1 (MQ=255)
                                   aaCCCCCCGGTACGGTTTATTACGTACATCAGGTAAAACTTACCCTTAAGCCGATTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAACCCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGa                                                                                                      <  2:282424/149‑1 (MQ=17)
                                          ctgAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGAc                                                                                               <  2:84183/147‑1 (MQ=255)
                                          cgGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGAc                                                                                               <  1:398015/149‑1 (MQ=255)
                                             aaCGTTTTATTACGTAAATCAGTTAAAACTGACCGATAAGTCGCTTTCTTTTGGGGATAGTGGCGTGGACAGTCATTCATCTTTATGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGa                                                                                            <  2:753883/149‑1 (MQ=14)
                                             aaCGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCATTTGGGAATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGa                                                                                            <  2:684701/149‑1 (MQ=255)
                                                ggTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATaa                                                                                         <  2:353749/149‑1 (MQ=255)
                                                  tttATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAg                                                                                                          >  2:744027/1‑130 (MQ=255)
                                                  gttattaCGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAg                                                                                                          <  1:744027/129‑1 (MQ=19)
                                                     attaCGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCATTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGc                                                                                    <  1:958261/149‑1 (MQ=255)
                                                       taCGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGt                                                                                                                 <  2:891747/118‑1 (MQ=25)
                                                       taCGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGt                                                                                                                 >  1:891747/1‑118 (MQ=25)
                                                          gTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGttttt                                                                                                                     >  1:731651/1‑111 (MQ=25)
                                                          gTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGttttt                                                                                                                     <  2:731651/111‑1 (MQ=25)
                                                             cataatttAACACTTACCGATAAGCCGCTTTCATGTGCTTTTAGTGTCGTCGACTGGGAATCATCTTTCGGCCCCTCCAAAAGTAAAAACCCGCCGAACCGGGTTTTTACGTAAAACAGGTCAAACTGACCGATAAGCCGCTTTCtttt                                                                            <  1:636857/142‑1 (MQ=11)
                                                             cataatgTAAAAATGACCGATAATCCGCTTTCTTTAGTTTTTAGTCTCGTGGACAGTCTTTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCtttt                                                                            <  1:59953/143‑1 (MQ=11)
                                                               tCAGGTAAAAATGAACGATCAGCCGCTTTCTTTTGGGGATTTTGTCCTTGTCAGTCATTCATCTTTCATCCCCTCCACAAGTACAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTgg                                                                          <  1:351502/149‑1 (MQ=11)
                                                                          tGACCGATATGCCGCTTTCTTTTGGTTATAGTGTCGTGGACAGTGATTCATCTTTCTGCCCCTCCAAAAGTATAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTTAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCg                                                               <  2:893825/149‑1 (MQ=11)
                                                                                     ccGCTAACTTTTTGGGATACCGTCGTGGAAAGTCATTCCTCTTTCTCCCCCTCAAAAAGTATAAACCCGCCGTAACGGTTTTTTACGTACAACAGGTCAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGtcat                                                    <  2:552391/149‑1 (MQ=11)
                                                                                                               ggACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGt                                                       >  2:463886/1‑120 (MQ=17)
                                                                                                                                       cTCAAAAATTTAAAACCCGCCTTATCGGTTTTTTACGTAAATCAGGTTAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTAc  <  2:400667/149‑1 (MQ=11)
                                                                                                                                          cAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAAc                                                                                                            >  2:209234/1‑40 (MQ=255)
                                                                                                                                                |                                                                                                                                           
CATCCGGCATGAATAAAGCGCACTCAACAATCTGAAACCCGCCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTAC  >  NC_000913/3269812‑3270095

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑