Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,406,717 C→T 7.1% I267I (ATC→ATT)  accC → acetyl‑CoA carboxylase, biotin carboxylase subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,406,7170CT 7.1% 142.0 / 2.8 56I267I (ATC→ATT) accCacetyl‑CoA carboxylase, biotin carboxylase subunit
Reads supporting (aligned to +/- strand):  ref base C (21/31);  new base T (2/2);  total (23/33)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.17e-01

TCAGGGCAACGCTATCTATCTGGCGGAACGTGACTGCTCCATGCAACGCCGCCACCAGAAAGTGGTCGAAGAAGCGCCAGCACCGGGCATTACCCCGGAACTGCGTCGCTACATCGGCGAACGTTGCGCTAAAGCGTGTGTTGATATCGGCTATCGCGGTGCAGGTACTTTCGAGTTCCTGTTCGAAAACGGCGAGTTCTATTTCATCGAAATGAACACCCGTATTCAGGTAGAACACCCGGTTACAGAAATGATCACCGGCGTTGACCTGATCAAAGAACAGCTGCGTATCGC  >  NC_000913/3406570‑3406863
                                                                                                                                                   |                                                                                                                                                  
tCAGGGCAACGCTATCTATCTGGCGGAACGTGACTGCTCCATGCAACGCCGCCACCAGAAAGTGGTCGAAGAAGCGCCAGCACCGGGCATTACCCCGGAACTGCGTCGCTACATCGGCGAACGTTGCGCTAAAGCGTGTGTTGATATCg                                                                                                                                                   >  2:419413/1‑149 (MQ=255)
    ggCAACGCTATCTATCTGGCGGAACGTGACTGCTCCATGCAACGCCGCCACCAGAAAGTGGTCGAAGAAGCGACAGCACCGGGCATTACACCGGAACTGCGTCGCAACATCCTCGAACGTTGCGCTAAAGCGTGTGTTGATATCGGATa                                                                                                                                               >  1:479754/1‑149 (MQ=255)
         cGCTATCTATCTGGCGGAACGTCACTGCTCCATGCAACGCCGCCACCAGAAAGTGGGCGAAGAAGCGCCAGCACCGGGCATTACCCCGGAACTGCGTCGCTACATCGGCGAACGTTGCGCTAAAGCGTGTGTTGATATCGGCTATcgcg                                                                                                                                          <  2:728913/149‑1 (MQ=255)
           ctatctatCTGGCGGCACGTGACTGCTCCATGCAACGCCGCCACCAGAAAGTGGTCGAAGAAGCGCCAGCACCGGGAATTACCCCGGAACTGCGTCGCTACATCGGCGAACGTTGCGCTAAAGCGTGTGTTGATATCGGCTATCGCGGt                                                                                                                                        <  1:808126/149‑1 (MQ=255)
                 tgctGGCGGAACGTGAGTGCTCCATGCACCGCCGCCACCAGATAGGGGTCGAAGAAGCGCCAGCACCGGGCATTACGCCGGAACTGCGTCGCTACATCGGCGAACGTTGCGCTAAAGCGTGTGTTGATATCGGCTATCGCGGTGCAGGt                                                                                                                                  <  1:81006/147‑1 (MQ=255)
                   ctGGCTGAACGTGTCTGCTCCATGCAACGCCGCAACCTGACAGTGGTCGAAGAAGCGCCAGCACCGGGCATTACCCCGGAACTGCGGCGCTACATCGGCGAACGTTGCGCTAAAGCGTGTGTTGATATCGGCTATCGCGGTGCAGGTAc                                                                                                                                <  2:758202/149‑1 (MQ=255)
                      gCGGACCGTGACTGCTACATGCAACGCCGACACCAGAAAGTGGTCGATGATGCGCCAGCACCGGGCATTACCCCGGTACTGCGTCGCTACATCGGCGAACGTTGCGCTAAAGGGTGTGTTGATATCGGCTTTCGCGGTGCTGGTACttt                                                                                                                             <  2:934576/149‑1 (MQ=255)
                      gCGGAACGTGACTGCTCCATGCAACGCCGCCACCAGAAAGTGGTCGAAGAAGCGCCAGCACCGGGCATTACCCCGGAACTGCGTCGCTACATCGGCGAACGTTGCGCTAAAGCGTGTGTTGATATCGGCTATCGCGGTGCAGGTACttt                                                                                                                             <  2:606627/149‑1 (MQ=255)
                        ggAACGTGACTGCTCCATGCAACGCCGCCACCAGAAAGTGGTCGAAGAAGCGCCAGCACCGGGCATTACCCCGGAACTGCGTCGCTACATCGGCGAACGTTGCGCTAAAGCGTGTGTTGATATCGGCTATCGCGGTGCAGGTACTTTCg                                                                                                                           >  2:762768/1‑149 (MQ=255)
                         gAACGTGACTGCTCCATTCAACGCCTCCACCAGAAAGTGGTCGAAGAAGCGCCAGCACCGGGCAGTACCCCGGAACTGCGTCGCTACATCGGCGAACGTTGCGCTAAAGCGTGTGATGATATCGGCTATCGCGGTGCAGGTACTTTCGa                                                                                                                          <  2:74968/149‑1 (MQ=255)
                             gTGACTGCTCCATGCAACGCCGCCACCAGAAAGTGGTCGAAGAAGCGCCAGCACCGGGCATTACCCCGGAACTGCGTCGCTACATCGGCGAACGTTGCGCTAAAGCGTGTGTTGATATCGGCTATCGCTGTGCAGGTACTTTCGAGTTc                                                                                                                      <  1:919886/149‑1 (MQ=255)
                             gTGACTGCTCCATGCAACGCCGCCACCAGAAAGTGGTCGAAGAAGCGCCAGCACCGGGCATTACCCCGGAACTGCGTCGCTACATCGGCGAACGTTGCGCTAAAGCGTGTGTTGATATCGGCTATCGCGGTGCAGGTACTTTCGAGTTc                                                                                                                      <  1:998313/149‑1 (MQ=255)
                              tGACTGCTCCATGCAACGCCGCCACCAGAAAGTGGTCGAAGAAGCGCCAGCACCGGGCATTACCCCGGAACTGCGTCGCTACATCGGCGAACGTTGCGCTAAAGCGTGTGTTGATATCGGCTATCGCGGTGCAGGTACTTTCGAGttac                                                                                                                     >  2:705728/1‑147 (MQ=255)
                                  tGCTCCATGCAACGCCGCCACCAGAAAGTGGTCGAAGAAGCGCCAGCACCGGGCATTACCCCGGACCTGCGTCGCTACATCGGCGAACGTTGCGCTAAAGCGTGTGTTGATATCGGCTATCGCGGTGCAGGTACTTTCGAGTTCCTGtt                                                                                                                 >  1:708403/1‑149 (MQ=255)
                                  tGCTCCATGCAACGCCGCCACCAGAAAGTGGTCGAAGAAGCGCCAGCACCGGGCATTACCCCGGAACTGCGTCGCTACATCGGCGAACGTTGCGCTAAAGCGTGTGTTGATTTCGGCTATCGCGGTGCATGTACTTTGGAGTTCCTGtt                                                                                                                 >  2:583651/1‑149 (MQ=255)
                                    cTCCATGCAACGCCGCCACCAGAAAGTGGTCGAAGAAGCGCCAGCACCGGGCATTACCCCGGAACTGCGTCGCTACATCGGCGAACGTTGCGCTAAAGCGTGTGTTGATATCGGCTATCGCGGTGCAGGTACTTTCGAGTTCCTGTTCg                                                                                                               <  1:29281/149‑1 (MQ=255)
                                           cAACGCCGCCACCAGAAAGTGGTCGAAGAAGCGCCAGCACCGGGCAGTCCCCCGGAACTGCGGCGCTACATCGGCGAACGTTGCGCTAAAGCGTGTGTTGATATCGGCTATCGCGGTGCAGGTACTTTCGAGTTCCTGTTCGAAAACgg                                                                                                        <  2:812999/149‑1 (MQ=255)
                                                     accaGAAAGTGGTCGAAGAAGCGCCAGCACCGGGCATTACCCCGGAACTGCGTCGCTACATCGGCGAACGTTGCGCTAAAGCGTGTGTTGATATCGGCTATCGCGGTGCAGGTACTTTCGAGTTCCTGTTCGAAAACGGCGAGTTCTAt                                                                                              <  2:945477/149‑1 (MQ=255)
                                                       caGAAAGTGGTCGAAGAAGCGCCAGCACCGGGCATTACCCCGGAACTGCGTCGCTACATCGGCGAACGTTGCGCTAAAGCGTGTGTTGATATCGGCTATCGCGGTGCAGGTACTTTCGAGTTCCTGTTCGAAAACGGCGAGTTCTAttt                                                                                            >  1:874352/1‑149 (MQ=255)
                                                       caGAAAGTGGTCGAAGAAGCGCCAGCACCGGGCATTACCCCGGAACTGCGTCGCTACATCGGCGAACGTTGCGCTAAAGCGTGTGTAGATATCGGATATCGCCGTAAAATTAAAAAAAAATTCCTGTTCGAAAACGGCGAGATCTAttt                                                                                            >  2:528081/1‑149 (MQ=255)
                                                               ggTCGAAGAAGCGCCAGCACCGGGCATTACCCCGGAACTGCGTCGCTACATCGGCGAACGTTGCGCTAAAGCGTGTGTTGATATCGGCTATCGCGGTGCAGGTACTTTCGAGTTCCTGTTCGAAAACGGCGAGTTCTATTTCATCGaaa                                                                                    <  2:830163/149‑1 (MQ=255)
                                                                gTCGAAGAAGCGCCAGCACCGGGCATTACCCCGGAACTGCGTCGCTACATCGGCGAACGTTGCGCTAAAGCGTGTGTTGATATTGGCTATCGCGGTGCAGGTACTTTCGAGTTCCTGTTCGAAAACGGCGAGTTCTATTTCATCGAAAt                                                                                   >  2:734066/1‑149 (MQ=255)
                                                                gTCGAAGAAGCGCCAGCACCGGGCATTACCCCGGAACTGCGTCGCTACATCGGCGAACGTTGCGCTAAAGCGTGTGTTGATATTGGCTATCGCGGTGCAGGTACTTTCGAGTTCCTGTTCGAAAACGGCGAGTTCTATTTCATCGAAAt                                                                                   >  2:830455/1‑149 (MQ=255)
                                                                 tCGAAGAAGCGCCAGCACCGGGCATTACCCCGGAACTGCGTCGCTACATCGGCGAACGTTGCGCTAAAGCGTGTGTTGATATCGGCTATCGCGGTGCAGGTACTTTCGAGGTCCTGTTCGAAAACGGCGAGTTCTATTTCATCGAAATg                                                                                  >  1:757265/1‑149 (MQ=255)
                                                                   gaagaaGCGCCAGCACCGGGCATTACCCCGGAACTGCGTCGCTACATCGGCGAACGTTGCGCTAAAGCGTGTGTTGATATCGGCTATCGCGGTGCAGGTACTTTCGAGTTCCTGTTCGAAAACGGCGAGTTCTATTTCATCGAAATGaa                                                                                >  1:2443/1‑149 (MQ=255)
                                                                   gaagaaGCGCCAGCACCGGGCATTACCCCGGAACTGCGTCGCTACATCGGCGAACGTTGCGCTAAAGCGTGTGTTGATATCGGCTATCGCGGGGCAGGTACTTTCGAGTTCCTGTTCGAAAACGGCGAGTTCTATTTCATCGAAATGaa                                                                                >  1:35982/1‑149 (MQ=255)
                                                                        tgcgcCATCACCGGTCATTACCCGGGAACTGCGTCGGTACATCGTCGAACGTTGCGCTAAAGCGTGTGTTGATATCGGCTATCGCGGTGCAGGTACTTTCGAGTTCCTGTTCGAAAACGTCGAGTTCTATTTCATCGAAATTAACAccc                                                                           <  2:114749/148‑1 (MQ=255)
                                                                           gcCAGCACCGGGCATTACCACGGAACTGCGTCGCTACATCGGCGAACGTTGCGCTAAAGCGTGTGTTGATATCGGCTATCGCGGTGCTGGTACTTTCGAGTTCCTGTTCGAAAACGGCGAGTTCTATTTCATCGAAATGAACACCCGTa                                                                        >  1:341684/1‑149 (MQ=255)
                                                                           gcCAGCACCGGGCATTAACACTGAACTGCGTCTCTTCATCGGCGAACGTTGCGCTAAAGCGTGTGTTGATATCGGCTATCGCGTGGCAGGGACTTTCGAGTTCCTGTTCGAAAACGGCGAGGTCTATTTCATCGAAATGAACACCCGTa                                                                        <  1:77489/149‑1 (MQ=255)
                                                                            ccAGCACCGGGCATTACCCCGGAACTGCGTCGCTACATCGGCGAACGTTGCGCTAAAGCGTGTGTTGATATCGGCTATCGCGGTGCAGGTACTTTCGAGTTCCTGTTCGAAAACGGCGAGTTCTATTTCATCGAAATGAACACCCGTAt                                                                       <  2:2443/149‑1 (MQ=255)
                                                                             cAGCACCGGGCATTACCCCGGAACTGCGTCGCTACATCGGCGAACGTTGCGCTAAAGCGTGTGTTGATATCGGCTATCGCGGTGCAGGTACTTTCGAGTTCCTGTTCGAAAACGGCGAGTTCTATTTCATCGAAATGAACACCCGTAtt                                                                      <  1:546784/149‑1 (MQ=255)
                                                                             cAGCACCGGGCATTACCCCGGAACTGCGTCGCTACATCGGCGAACGTTGCGCTAAAGCGTGTGTTGATATCGGCTATCGCGGTGCAGGTACTTTCGAGTTCCTGTTCGAAAACGGCGAGTTCAATTTCATCGAAATGAACACCCGTAtt                                                                      <  1:419413/149‑1 (MQ=255)
                                                                              aGCACCGGGCATTACCCCGGAACTGCGTCGCTACATCGGCGAACGTTGCGCTAAAGCGTGTGTTGATATCGGCTATCGCGGTGCAATTACATTCTAGTTCCTGTTCGAAAACGGCGAGTTCTATTTCATCGAAATGAACACCCGTATTc                                                                     >  2:393517/1‑149 (MQ=255)
                                                                                  ccGGGCATTACCCCGGAACTGCGTCGCTACATCGGCGAACGTTGCGCTAAAGCGTGTGTTGATATCGGCTATCGCGGTGCAGGTACTTTCGAGTTCCTGTTCGAAAACGGCGAGTTCTATTTCATCGAAATGAACACCCGTATTCAGGt                                                                 <  1:199577/149‑1 (MQ=255)
                                                                                    gggCATTACCCCGGAACTGCGTCGCTACATCGGCGAACGTTGCGCTAAAGCGTGTGTTGATATCGGCTATCGCGGTGCAGGTACTTTCGAGATCCTGTTCGAAAACGGCGAGTTCTATTTCATCGAAATGAACACCCGTATTCAGGTAg                                                               <  1:774865/149‑1 (MQ=255)
                                                                                         ttACCCCGGAACTGCGTCGCTACATCGGCGAACGTTGCGCTAAAGCGTGTGTTGATATCGGCTATCGCGGTGCAGGTACTTTCGAGTTCCTGTTCGAAAACGGCGAGTTCTATTTCATCGAAATGAACACCCGTATTCAGGTAGAacac                                                          <  1:56029/149‑1 (MQ=255)
                                                                                          tACCCCGGAACTGCGTCGCTACATCGGCGAACGTTGCGCTAAAGCGTGTGTTGATATCGGCTATCGCGGTGCAGGTACTTTCGAGTTCCTGTTCGAAAACGGCGAGTTCTATTTCATCGAAATGAACACCCGTATTCAGGTAGAACAcc                                                         <  2:997077/149‑1 (MQ=255)
                                                                                              ccGGAACTGCGGCGGTACTTCGGCGAACGTTGCGCTAAAGCGTGTGTTGATATCGGCTATCGCGGTGCAGGTACTTTCGATTTGCTGTTCGAAAACGGCGAGTTCTATTTCATCGAAATGAACACCCGTATTCAGGTATAACACCCGGt                                                     <  2:479754/149‑1 (MQ=255)
                                                                                                    ttccggcGCTACATCGGCGTCCGTGGCGATAAAGCGTGTGTTTATATCGCCTTTGGCGGTGCAGTTACTTACGTGTTCCTGTTCTAAAACGGCTAGTTATATTTCATCGAAATGAACACCCGTATTCAGGTAGAACACCCGGTTACAGa                                               <  2:84710/143‑1 (MQ=255)
                                                                                                       cgactcTACATCGGCGAACGTTGCGCTAAAGCTTGTGTCGATATCGGCTATCGCGGTGCAGGTAATTTCGAGTTCCTGTTCGAAAACGGAGAGTTCTATTTCATCGAAATGAACACCCATATTCAGGTAGAACACCCGGTTACAGAAAt                                            <  2:849831/144‑1 (MQ=255)
                                                                                                          cGCTACATCGGCGAACGTTGCGCTAAAGCGTGTGTTGATATCGGCTATCGCGGTGCAGGTACTTTCGAGTTCCTGTTCGAAAACGGCGAGTTCTATTTCATCGAAATGAACACCCGTATTCAGGTAGAACACCCGGTTACAGAAATGAt                                         <  1:762768/149‑1 (MQ=255)
                                                                                                            cTACATCGGCGAACGTTGCGCTAAAGCGTGTGTTGATATCGGCTATCGCGGTGCAGGTACTTTCGAGTTCCTGTTCGAAAACGGCGAGTTCTATTTCATCGAAATGAACACCCGTATTCAGGTAGAACACCCGGTTACAGAAATGATCa                                       >  1:139837/1‑149 (MQ=255)
                                                                                                                aTCGGCGAACGTTGCGCTAAAGCGTGTGTTGATATCGGCTATCGCGGTGCAGGTACTTTCGAGTTCCTGTTCGAAAACGGCGAGTTCTATTTCATCGAAATGAACACCCGTATTCAGGTAGAACACCCGGTTACAGAAATGATCACCgg                                   >  1:722568/1‑149 (MQ=255)
                                                                                                                    gCGAACGTTGCGCTAAAGCGTGTGTTGATATCGGCTATCGCGGTGCAGGTACTTTCGAGTTCCTGTTCGAAAACGGCGAGTTCTATTTCATCGAAATGAACACCCGTATTCAGGTAGAACACCCGGTTACAGAAATGATCACCGGCGtt                               >  1:220160/1‑149 (MQ=255)
                                                                                                                            ttcgcTAATGCGTGTGTAGATATCGGCTATCGCGGTGCAGGTACTTTCGAGTTCCTGTTCGAAAACGGCGAGTTCTATTTCATCGAAATGAACACCCGTATTCAGGTAGAACACGCGGTTTCAGAAATGTTCACCGGCGTTGACCTGAt                       <  1:238508/147‑1 (MQ=255)
                                                                                                                               gcTAAAGCGTGTGTTGATATCGGCTATCGCGGTGCAGGTACTTTCGAGTTCCTGTTCGAAAACGGCGAGTTCTATTTCATCGAAATGAACACCCGTATTCAGGTAGAACACCCGGTTACAGAAATGATCACCGGCGTTGACCTGATCaa                    >  2:882262/1‑149 (MQ=255)
                                                                                                                                 tAAAGCGTGTGTTGATATCGGCTATCGCGGTGCAGGTACTTTCGAGTTCCTGTTCGAAAACGGCGAGTTCTATTTCATCGAAATGAACACCCGTATTCAGGTAGAACACCCGGTTACAGAAATGATCACCGGCGTTGACCTGATCAAAg                  >  1:645348/1‑149 (MQ=255)
                                                                                                                                 tAAAGCGTGTGTTGATATCGGCTATCGCGGTGCAGGTACTTTCGAGTTCCTGTTCGAAAACGGCGAGTTCTATTTCATCGAAATGAACACCCGTATTCAGGTAGAACACCCGGTTACAGAAATGATCACCGGCGTTGACCTGATCAAAg                  <  1:297205/149‑1 (MQ=255)
                                                                                                                                   aaGCGTGTGTTGATATTGGCTATCGCGGTGCAGGTACTTTCGAGTTCCTGTTCGAAAACGGCGAGTTCTATTTCATCGAAATGAACACCCGTATTCAGGTAGAACACCCGGTTACAGAAATGATCACCGGCGTTGACCTGATCAAAGaa                <  1:734066/149‑1 (MQ=255)
                                                                                                                                   aaGCGTGTGTTGATATTGGCTATCGCGGTGCAGGTACTTTCGAGTTCCTGTTCGAAAACGGCGAGTTCTATTTCATCGAAATGAACACCCGTATTCAGGTAGAACACCCGGTTACAGAAATGATCACCGGCGTTGACCTGATCAAAGaa                <  1:830455/149‑1 (MQ=255)
                                                                                                                                   aaGCGTGTGTTGATATCGGCTATCGCCGTCGATGTACATTCTACTTCCGGTTCGAAAACGGCGATTTCTATTTCATCGAAATGAACACCCGTATTCAGGTAGCACACCCGGTTAAAGAAATGATCCCCGGCGTTGACCTGATCAAAGaa                >  2:562602/1‑149 (MQ=255)
                                                                                                                                   aaGCGGGTGTTGATAGCGGCTAGCGCGGGGCAGGGACTTTCGAGTTCCTGTTCGAAAACGGCGAGTTCTATTTCCTCGAAATGAACACCCGTATTCAGGTAGAACACCCGGTTACAGAAATGATCACCGGCGTGGACCTGATCAAAGaa                <  1:777249/149‑1 (MQ=255)
                                                                                                                                           gtTGATATCGGCTATCGCGGTGCAGGTACTTTCGAGTTCCTGTTCGAAAACGGCGAGTTCTATTTCATCGAAATGAACACCCGTATTAAGGTAGAACACCCGGTTACTGAAATGATCACCGGCGTTGACAGGCTCTAAGAACAGCTGCg        >  2:666700/1‑149 (MQ=255)
                                                                                                                                              gctatCGCCTATCGCGGTCCAGGCACTTTCGAGTTCCTGTTCGAAAACGGCGAGTTCTATTTCATCGAAATCAACACCCGTATTCAGGTAGATCACCCTGTTACAGAAATGATCACCGGCGTTGACCTGATCAAAGAACAGCTGCGTAt     <  2:708403/147‑1 (MQ=255)
                                                                                                                                                tatCGGCTATCGCGGTGCAGGTACTTTCGAGTTCCTGTTCGAAAAAGGCGAGTTCTATTTCATCGAAATGAACACCCGTATTCAGGTAGAACACCCGGTTACAGAAATGATCACCGGCGTTGACCTGATCAAAGAACAGCTGCGTATcc   >  1:682899/1‑148 (MQ=255)
                                                                                                                                                 atCGGCTATCGCGGTGCAGGTACTTTCGATTTCCTGTTCGAAAACGGCGAGTTCTATTTCATCGAAATGAACACCCGTATTCAGGTAGAACACCCGGTTACAGAAATGATCACCGGCGTTTACCTGATCAAAGAACAGCTGCGTATCGc  <  1:518205/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
TCAGGGCAACGCTATCTATCTGGCGGAACGTGACTGCTCCATGCAACGCCGCCACCAGAAAGTGGTCGAAGAAGCGCCAGCACCGGGCATTACCCCGGAACTGCGTCGCTACATCGGCGAACGTTGCGCTAAAGCGTGTGTTGATATCGGCTATCGCGGTGCAGGTACTTTCGAGTTCCTGTTCGAAAACGGCGAGTTCTATTTCATCGAAATGAACACCCGTATTCAGGTAGAACACCCGGTTACAGAAATGATCACCGGCGTTGACCTGATCAAAGAACAGCTGCGTATCGC  >  NC_000913/3406570‑3406863

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑