Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,437,908 T→A 6.4% intergenic (+14/‑116) trkA → / → mscL NAD‑binding component of TrK potassium transporter/mechanosensitive channel protein, high conductance

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,437,9080TA 6.4% 300.0 / 6.8 116intergenic (+14/‑116)trkA/mscLNAD‑binding component of TrK potassium transporter/mechanosensitive channel protein, high conductance
Reads supporting (aligned to +/- strand):  ref base T (45/63);  new base A (3/5);  total (48/68)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.61e-01

Only 100 of 117 total aligned reads displayed.

CGTGGAAACGACGTGATGATTGCCAATGACAATCTGCGCATTGAGCAAGGCGATCACGTAATTATGTTCCTCACAGATAAAAAGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGTTAGACTTATGGTTGTCGGCTTCATAGGGAGAATAACATGAGCATTATTAAAGAATTTCGCGAATTTGC  >  NC_000913/3437760‑3438055
                                                                                                                                                    |                                                                                                                                                   
cGTGGAAACGACGTGATGATTGCCAATGACAATCTGCGCATTGAGCAAGGCGATCACGTAATTATGTTCCTCACAGATAAAAAGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCt                                                                                                                                                     <  2:1018537/149‑1 (MQ=255)
  tGGAAACGACGTGATGATTGCCAATGACAATCTGCGCATTGAGCAAGGCGATCACGTAATTATGTTCCTCACAGATAAAAAGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTaa                                                                                                                                                   >  2:980472/1‑149 (MQ=255)
  tGGAAACGACGTGATGATTGCCAATGACAATCTGCGCATTGAGCAAGGCGATCACGTAATTATGTTCCTCACAGATAAAAAGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTaa                                                                                                                                                   >  2:27061/1‑149 (MQ=255)
    gAAACGAGGTGATGATTGCCAATGACAATCTGCGCAGTGAGCAAGGAGATCACGTAATTATGTTCCTCACAGATAAAAAGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTGCTTCTTGTAATTAATAAGGCGTCTAATg                                                                                                                                                 >  2:723823/1‑149 (MQ=255)
     aaaCGACGTGATGATTGCCAATGACAATCTGCGCATTGAGCAAGGCGATCACGTAATTATGTTCCTCACAGATAAAAAGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGa                                                                                                                                                <  1:109919/149‑1 (MQ=255)
              gatgatTGCCAATGACAATCTGCGCATTGAGCAAGGCGATCACGTAATTATGTTCCTCACAGCTAAAAAGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCATTAGACGCCttatt                                                                                                                                       <  2:483012/149‑1 (MQ=255)
              gatgatTGCCAATGACAATCTGCGCATTGAGCAAGGCGATCACGTAATTATGTTCCTCACAGATAATAAGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCttatt                                                                                                                                       <  2:115054/149‑1 (MQ=255)
              gatgatTGCCAATGACAATCTGCGCATTGAGCAAGGCGATCACGTAATTATGTTCCTCACAGATAAAAAGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCttatt                                                                                                                                       >  1:3169/1‑149 (MQ=255)
              gatgatTGCCAATGACAATCTGCGCATTGAGCAAGGCGATCACGTAATTATGTTCCTCACAGATAAAAAGTTTATAACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCttatt                                                                                                                                       >  1:876263/1‑149 (MQ=255)
               atgatTGCCAATGACAATCTGCGCATTGAGCAAGGCGATCACGTAATTATGTTCCTCACAGATAAAAAGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCttatta                                                                                                                                      <  2:608848/149‑1 (MQ=255)
                tgatTGCCAATGACAATCTGCGCATTGAGCAAGGCGATCACGTAATTATGTTCCTCACAGATAAAAAGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCATTAGACGCCttattaa                                                                                                                                     <  2:882971/149‑2 (MQ=255)
                 gatTGCCAATGACAATCTGCGCATTGAGCAAGGCGATCACGTAATTATGTTCCTCACAGATAAAAAGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTattatt                                                                                                                                    >  2:27715/1‑149 (MQ=255)
                  atTGCCAATGACAATCTGCGCATTGAGCAAGGCGATCACGTAATTATGTTCCTCACAGATAAAAAGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTAttt                                                                                                                                   >  2:637481/1‑149 (MQ=255)
                  atTGCCAATGACAATCTGCGCATTGAGCAAGGCGATCACGTAATTATGTTCCTCACAGATAAAAAGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTAttt                                                                                                                                   >  2:981039/1‑149 (MQ=255)
                  atTGCCAATGACAATCTGCGCATTGAGCAAGGCGATCACGTAATTATGTTCCTCACAGATAAAAAGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTAttt                                                                                                                                   >  1:742984/1‑149 (MQ=255)
                   ttGCCAATGACAATCTGCGCATTGAGCAAGTCGATCACGTAATTATGTTCCTCACAGATAAAAAGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGAGGCCTTATTATTTc                                                                                                                                  >  1:325966/1‑149 (MQ=255)
                   ttGCCAATGACAATCTGCGCATTGAGCAAGGCGATCACGTAATTATGTTCCTCACAGATAAAAAGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCATTAGACGCCTTATTAatta                                                                                                                                  <  1:1007968/149‑2 (MQ=255)
                   ttGCCAATGACAATCTGCGCATTGAGCAAGGCGATCACGTAATTATGTTCCTCACAGATAAAAAGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCATTCTTCTTGTAATTAATAAGGCGTCATTAGACGCCTTATTAatta                                                                                                                                  <  1:167076/149‑2 (MQ=255)
                   ttGCCAATGACAATCTGCGCATTGAGCAAGGCGATCACGTAATTATGTACCTCACAGATAAAAAGTTTATTACAGACGTCCAAAGACTCTTCCATCAAAGCCATTTATTCTTGTAATTAATAATGCGAATAAATACGCCatataaatta                                                                                                                                  >  2:98537/1‑143 (MQ=255)
                     gCCAATGACAATCTGCGCATTGAGCAAGGCGATCACGTAATTATGTTCCTCACAGATAAAAAGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTccc                                                                                                                                <  1:459142/149‑1 (MQ=255)
                      ccAATGACAATCTGCGCATTGAGCAAGGCGATCACGTAATTATGTTCCTCACAGATAAAAAGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCt                                                                                                                               >  1:96825/1‑149 (MQ=255)
                      ccAATGACAATCTGCGCATTGAGCAAGGCGATCACGTAATTATGTTCCTCACAGATAAAAAGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGGCATTAGACGCCTTATTAattacaa                                                                                                                               <  1:837338/149‑5 (MQ=255)
                      ccAATGACAATCTGCGCATTGAGCAAGGCGATCACGTAATTATGTTCCTAACAGATAAAAAGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAAAGACGCCTTATTATTTCCCt                                                                                                                               <  2:314138/149‑1 (MQ=255)
                       cAATGACAATCTGCGCATTGAGCAAGGCGATCACGTAATTATGTTCCTCACAGATAAAAAGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCtt                                                                                                                              <  1:637481/149‑1 (MQ=255)
                        aaTGACAATCTGCGCATTGAGCAAGGCGATCACGTAATTATGTTCCTCACAGATAAAAAGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCttt                                                                                                                             <  2:337570/149‑1 (MQ=255)
                         aTGACAATCTGCGCATTGAGCAAGGCGATCACGTAATTATGTTCCTCACAGATAAAAAGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTg                                                                                                                            >  2:124574/1‑149 (MQ=255)
                          tGACAATCTGCGCATTGAGCAAGGCGATCACGTAATTATGTTCCTCACAGATAAAAAGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGa                                                                                                                           <  2:156878/149‑1 (MQ=255)
                          tGACAATCTGCGCATTGAGCAAGGCGATCACGTAATTATGTTCCTCACAGATAAAAAGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGa                                                                                                                           <  1:642348/149‑1 (MQ=255)
                           gACAATCTGCGCATTGAGCAAGGCGATCACGTAATAATGTTCCTCACAGATAAAAAGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGAt                                                                                                                          <  1:975508/149‑1 (MQ=255)
                             cAATCTGCGCATTGAGCAAGGCGATCACGTAATTATGTTCCTCACAGATAAAAAGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCGTTCTTCTAGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTa                                                                                                                        >  2:428921/1‑149 (MQ=255)
                              aaTCTGCGCATTGAGCAAGGCGATCACGTAATTATGTTCCTCACAGATAAAAAGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTAt                                                                                                                       <  1:127783/149‑1 (MQ=255)
                                tCTGCGCATTGAGCAAGGCGATCACGTAATTATGTTCCTCACAGATAAAAAGTTTATTAACTACGTCGAAAGACTCTTCCAGCCAAGCCCGTTCTTCTTGTAATTAATAAGGCGTCTAATGACTCCTTATTATTTCCCTTTGATTATCa                                                                                                                     >  2:926680/1‑149 (MQ=255)
                                 cTGCGCATTGAGCAAGGCGATCACGTAATTATGTTCCTCACAGATAAAAAGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCaa                                                                                                                    <  2:319441/149‑1 (MQ=255)
                                     gcATTCAGCAAGGCGATCACGTAATTATGTTCCTGACAGATAAAAAGTTTATTACAGACGTAGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGat                                                                                                                <  1:85448/149‑1 (MQ=255)
                                       aTTGAGCAAGGCGATCACGTAATTATGTTCCTCACAGATAAAAAGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGatta                                                                                                              >  1:871357/1‑149 (MQ=255)
                                         tGAGCAAGGCGATCACGTAATTATGTTCCTCACAGATATAAAGTTTATTACCGACGTCGAAAGACTCTTCCATCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGattaat                                                                                                            <  1:99558/149‑1 (MQ=255)
                                             cAAGGCGATCACGTAATTATGTTCCTCACAGATAAAAAGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTaaa                                                                                                        <  2:471085/149‑1 (MQ=255)
                                             cAAGGCGATCACGTAATTATGTTCCTCACAGATAAAAAGTTTATTACCGACGTCGAAAGACTCTTCCAGCAAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTaaa                                                                                                        <  1:425241/149‑1 (MQ=255)
                                                        cGTAATTATGTTCCTCACAGATAAAAAGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTgg                                                                                             >  2:736711/1‑149 (MQ=255)
                                                        cGTAATTATGTTCCTCACAGATAAAAAGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTgg                                                                                             >  2:739969/1‑149 (MQ=255)
                                                          tatttATTTTCATCACAGATAAAAAGTTTATTACAGACGTCGAAAGACTCTTCCAGCCATGACCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACTCCTTAATATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCa                                                                                           <  2:64446/146‑1 (MQ=255)
                                                          tAATTATGTTCCTCACAGATAAAAAGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCa                                                                                           <  1:499409/149‑1 (MQ=255)
                                                                   tCCTCACAGATAAAAAGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATgg                                                                                  <  2:713218/149‑1 (MQ=255)
                                                                    ccTCACAGATAAAAAGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGc                                                                                 <  1:147918/149‑1 (MQ=255)
                                                                      tCACAGATAAAAGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTa                                                                              >  2:861765/1‑149 (MQ=255)
                                                                        acaGATAAAAAGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTATTGACGCCTTATTATTTCCCTTTGATTATAAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGTCTTaa                                                                             >  1:307447/1‑149 (MQ=255)
                                                                         caGATAAAAAGTTTATTACCGACTTCGAAATACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAAc                                                                            <  1:226310/149‑1 (MQ=255)
                                                                          aGATACAAAGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCATGTAATTAATAATGCGACTAATGACGCCTTATTATTTACCTTTGATTATCAACGATTAATAAAATTCATTCCTGGCAGCAAAATGGCTTAACa                                                                           <  2:469105/149‑1 (MQ=255)
                                                                          aGATAAAAAGTTTATTACCGACGTCGAAAGACTCTTCCAGCCACGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATCTCGCCTTATTATTTCCCTTTGTTTATCAAGGATTATTTAAATTCATTCCTGGCAGGAAATTGGCGTAACa                                                                           >  1:682063/1‑149 (MQ=255)
                                                                          aGATAAAAAGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTACCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACa                                                                           <  2:3169/149‑1 (MQ=255)
                                                                             tAAAAAGTATAATACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCATCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTAACAAGCATTAATTAAATTCAAACATGGCAGGAAATTGGCTTAAAAttt                                                                        >  2:432786/1‑149 (MQ=255)
                                                                               aaaaGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGt                                                                      <  1:848576/149‑1 (MQ=255)
                                                                               aaaaGTTTATTACAGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTTATAAGGCGTCTAATGACGCCTTATGTTTTGCCTTTGATTATCTAGGATTAATTAAATTCATTACTGGCAGGAAAATGGCTTAACATTTgg                                                                      >  2:498568/1‑148 (MQ=255)
                                                                                aaaGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGtt                                                                     <  1:121460/149‑1 (MQ=255)
                                                                                 aaGTTTATTTCCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTGTTGTAATTAATAATGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGTTa                                                                    <  1:510527/149‑1 (MQ=255)
                                                                                   gTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGTTAGa                                                                  <  1:211029/149‑1 (MQ=255)
                                                                                   gTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGTTAGa                                                                  <  1:498739/149‑1 (MQ=255)
                                                                                      tattaCCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATACGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATGAAGGATTAATTTAATTAATTCGTGGGAGGTAAATGGCTTTTCATTTTTTAGACtt                                                               >  2:919986/1‑149 (MQ=255)
                                                                                      tattaCCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGTTAGACtt                                                               <  1:408306/149‑1 (MQ=255)
                                                                                      tattaCCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGTTAGACtt                                                               >  2:923846/1‑149 (MQ=255)
                                                                                          aCCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGTTAGACTTATgg                                                           <  1:810409/149‑1 (MQ=255)
                                                                                                   gAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTAATTCCTGGCAGTAAAATGGCTTAACATTTGTTAGACTTATGGTTGTCGGCt                                                  >  1:1019041/1‑149 (MQ=255)
                                                                                                    aaaGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGTTAGACTTATGGTTGTCGGCtt                                                 >  1:775159/1‑149 (MQ=255)
                                                                                                      tgACTCTTCCAGCCAATCCCTTTCTTCTTGTTATTAATAAGGCGTATAATGACGCCTTATTATTTCCCTTTGTTTATCAAGGATTAATTAAATTCATTGCTGGCAGGAAAATGGCTTAACATTTGTTAGACTTATGGTTGTCGTCTTCa                                               <  1:98537/148‑1 (MQ=255)
                                                                                                       taCTCTTACAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGTTAGACTTATGGTTGTCGGCTTCAt                                              <  2:208869/148‑1 (MQ=255)
                                                                                                       gACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGTTAGACTTATGGTTGTCGGCTTCAt                                              <  1:587100/149‑1 (MQ=255)
                                                                                                        aCTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGTTAGACTTATGGTTGTCGGCTTCATa                                             >  1:1014849/1‑149 (MQ=255)
                                                                                                          tctTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGTTAGACTTATGGTTGTCGGCTTCATAgg                                           >  1:1009966/1‑149 (MQ=255)
                                                                                                          tctTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGTTAGACTTATGGTTGTCGGCTTCATAgg                                           <  2:876263/149‑1 (MQ=255)
                                                                                                           ctTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGTTAGACTTATGGTTGTCGGCTTCATAggg                                          <  1:39172/149‑1 (MQ=255)
                                                                                                           ctTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGTTAGACTTATGGTTGTCGGCTTCATAggg                                          <  1:42872/149‑1 (MQ=255)
                                                                                                           ctTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGTTAGACTTATGGTTGTCGGCTTCATAggg                                          <  1:798739/149‑1 (MQ=255)
                                                                                                           ctTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGTTAGACTTATGGTTGTCGGCTTCATAggg                                          <  1:933067/149‑1 (MQ=255)
                                                                                                            ttCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGTTAGACTTATGGTTGTCGGATTCATAGGga                                         >  1:746934/1‑149 (MQ=255)
                                                                                                            ttCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAAAATTTGTTAGACTTATGGTTGTCGGCTTCATAGGga                                         >  2:18924/1‑149 (MQ=255)
                                                                                                                   cAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGTTAGACTTATGGTTGTCGGCTTCATAGGGAGAATAAc                                  <  1:629043/149‑1 (MQ=255)
                                                                                                                     aGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAATGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGTTAGACTTATGGTGGTCGGCTTCATAGGGAGAATAACAt                                <  2:717334/149‑1 (MQ=255)
                                                                                                                     aGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGTTAGACTTATGGTTGTCGGCTTCATAGGGAGAATAACAt                                <  1:981039/149‑1 (MQ=255)
                                                                                                                      gCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGTTAGACTTATGGTTGTCGGCTTAATAGGGAGAATAACAtt                               >  2:59394/1‑148 (MQ=255)
                                                                                                                       cccTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGTTAGACTTATGGTTGTCGGCTTCATAGGGAGAATAACATGa                              <  1:861765/149‑1 (MQ=255)
                                                                                                                         cTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGTTAGACTTATGGTTGTCGGCTTCATAGGGAGAATAACATGAGc                            <  1:739969/149‑1 (MQ=255)
                                                                                                                          tttCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATGTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGTTAGACTTATGGTTGTCGGCTTCATAGGGAGAATAACATGAGCa                           >  1:563308/1‑149 (MQ=255)
                                                                                                                                attGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATTGCTTAACATTTGTTAGACTTATGGTTGTCGGCTTCATTGGGAGAATAACATGAGCAttatta                     <  1:27061/148‑1 (MQ=255)
                                                                                                                                 ttGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGTTAGACTTATGGTTGTCGGCTTCATAGGGAGAATAACATGAGCATTATTaa                    >  2:357670/1‑149 (MQ=255)
                                                                                                                                  tGTAATTAATTAGTCGTCTAATGTCGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGTAAATGGCTTAACCTTTGTTAGACTTATGGTTGGCGGCGTCATAGGGAGAATAACATGAGCATTATTaaa                   <  1:664659/149‑1 (MQ=255)
                                                                                                                                  tGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGTTAGACTTATGGTTGTCGGCTTCATAGGGAGAATAACATGAGCATTATTaaa                   <  2:187984/149‑1 (MQ=255)
                                                                                                                                      attaatAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGTTAGACTTATGGTTGTCGGCTTCATAGGGAGAATAACATGAGCATTATTAAAGAAt               >  1:238489/1‑149 (MQ=255)
                                                                                                                                       ataataaGGCGTCATTTGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAAATAAATTCATTACTGGCAGGAAAATGGCTTAACATTTGTTAGACTTATGGTTGACGGCTTCATAGGGTGAATAACATGAGCATTATTAAAGAAtt              >  2:701340/2‑149 (MQ=255)
                                                                                                                                         aataaGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGTTAGACTTTTGGTTGTCGGCTTCATAGGGAGAATAACATGAGCATTATTAAAGAATTTc            >  1:347834/1‑149 (MQ=255)
                                                                                                                                         aataaGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGTTAGACTTATGGTTGTCGGCTTCATAGGGAGAATAACATGAGCATTATTAAAGAATTTc            <  1:973865/149‑1 (MQ=255)
                                                                                                                                         aataaGGCGTCATTAGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAAGTAAATTCATTACTGGCAGGAAAATGGCTTAACAGTTGTTAGACTTATGGTTGTCGGCTTCATAGGGAGAATAACATGAGCATTATTAAAGAAtttg            >  1:557231/1‑148 (MQ=255)
                                                                                                                                          ataaGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGTTAGACTTATGGTTGTCGGCTTCATAGGTAGAATAACATGAGCATTATTAAAGAATTTcg           >  2:163164/1‑149 (MQ=255)
                                                                                                                                          ataaGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGTTAGACTTATGGTTGTCGGCTTCATAGGGAGAATAACATGAGCATTATTAAAGAATTTcg           <  1:923846/149‑1 (MQ=255)
                                                                                                                                           taaGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTTGCAGGAAAATGGCTTAACATTTGTTAGACTTATGGTTGTCTGCTTCATAGGGAGAATAACATTAGCATTATTAAAGAATTTcgc          >  2:165818/1‑149 (MQ=255)
                                                                                                                                           taaGGCGTCATTAGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGTTAGACTTATGGTTGTCGGCTTCATAGGGAGAATAACATGAGCATTATTAAAGAATTTcgc          >  1:479505/1‑149 (MQ=255)
                                                                                                                                            acggcggcTACTGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGTTAGACTTATGGTTGTCGGCTTCATAGGGAGAATAACATGAGCATTATTAAAGAATTTcgcg         <  2:946341/147‑1 (MQ=255)
                                                                                                                                             aGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGTTAGACTTATGGTTGTCGGCTTCATAGGGAGAATAACATGAGCATTATTAAAGAATTTCGCGa        >  1:287247/1‑149 (MQ=255)
                                                                                                                                                  tCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTGCTGGCAGGAAAATGGCTTAACATTTGTTAGACTTATGGTTGTCGGCTTCATAGGGAGAATAACATGAGCATTATTAAAGAATTTCGCGAATTTg   <  2:96825/149‑1 (MQ=255)
                                                                                                                                                  tCTAATGACGAATTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGTCATGAAAATGGCTTAAAATTTGTTAGACTTATTTTTGTCGGCTTCATAGGTAGAATAACATGAGCATTATTAAAGAATTTCGCGAATTTg   <  2:471867/149‑1 (MQ=255)
                                                                                                                                                   cTAATGACGCCTTATTATTTGCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGTTAGACTTATGGTTGTCGGCTTCATAGGGAGAATAACATGAGCATTATTAAAGAATTTCGCGAATTTGc  <  1:428921/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
CGTGGAAACGACGTGATGATTGCCAATGACAATCTGCGCATTGAGCAAGGCGATCACGTAATTATGTTCCTCACAGATAAAAAGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGTTAGACTTATGGTTGTCGGCTTCATAGGGAGAATAACATGAGCATTATTAAAGAATTTCGCGAATTTGC  >  NC_000913/3437760‑3438055

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: