Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 346,566 C→T 5.1% A28V (GCG→GTG)  yahO → periplasmic protein, function unknown, YhcN family

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913346,5660CT 5.1% 194.3 / 2.1 79A28V (GCG→GTG) yahOperiplasmic protein, function unknown, YhcN family
Reads supporting (aligned to +/- strand):  ref base C (41/33);  new base T (2/2);  total (44/35)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.32e-01

CTAATTATTAATCAACCCAATAATGTGGGTGGGTGATAGTGTGATAACAACTCTGGAGCCGTAATATGAAAATAATCTCTAAAATGTTAGTCGGTGCGTTAGCGTTAGCCGTTACCAATGTCTATGCCGCTGAATTGATGACCAAAGCGGAATTTGAAAAAGTTGAATCGCAGTATGAAAAAATAGGTGATATTTCAACCAGCAATGAAATGTCGACTGCAGATGCAAAAGAAGATTTGATCAAAAAAGCGGATGAAAAAGGGGCTGATGTGTTGGTACTGACCTCCGGTCAAACT  >  NC_000913/346419‑346714
                                                                                                                                                   |                                                                                                                                                    
cTAATTATTAATCAACCCAATAATGTGGGTGGGTGATAGTGTGATAACAACTCTGGAGCCGTAATATGAAAATAATCTCTAAAATGTTAGTCGGTGCGTTAGCGTTAGCCGTTACAAATGTCTATGCCGCTGAATTGATGACCAAAGCg                                                                                                                                                     >  2:496801/1‑149 (MQ=255)
      tttaATCAACCCATTAATGTGGTTGGGTGATAGTTTGATAACATCTCTGGAGCCGTAATATGAAAATAATCTCTAAAATGTTCGTCGGTGCGTTAGCGTTAGCCGTTACCAATGTCTATGCCGCTGAATTGATGACCAAAGCGGAAttt                                                                                                                                               <  1:161717/148‑1 (MQ=255)
      attaATCAACCCAATAATGTGGGTGGGTGATAGTGTGATAACAACTCTGGAGCCGTAATATGAAAATAATCTCTAAAATGTTAGTCGGTGACATAGCGTTAGCCCTTACCAATGTCTATGCCGCGGAATTGATGACCAAAGCGGAAttt                                                                                                                                               >  1:900984/1‑149 (MQ=255)
       ttaATCAACCCAATAATGTGGGTGGGTGATAGTGTGATAACAACTCTGGAGCCGTAATATGAAAATAATCTCTAAAATGTTAGTCGGTGCGTTAGCGTTAGCCGTTACCAATGTCTATGCCGCTGAATTGATGACCAAAGCGGAATTTg                                                                                                                                              <  1:915035/149‑1 (MQ=255)
        taATCAACCCAATAATGTGGGTGGGTGATAGTGTGATAACAACTCTGGAGCCGTAATATGAAAATAATCTCTAAAATGTTAGTCGGTGCGTTAGCGTTAGCCATTACCAATGTATATGCCGCTGAATTGATGACCAAAGCGGAAATTGa                                                                                                                                             >  2:836423/1‑149 (MQ=255)
          aTCAACCCAATAATGTGGGTGGGTGATAGTGTGATAACAACTCTGGAGCCGTAATATGAAAATAATCTCTAAAATGTTAGTCGGTGCGTTAGCGTTAGCCGTTACCAATGTCTATGCCGCTGAATTGATGACCAAAGCGGAATTTGaaa                                                                                                                                           >  1:494540/1‑149 (MQ=255)
          aTCAACCCAATAATGTGGGTGGGTGATAGTGTGATAACAACTCTGGAGCAGTAATATGAAAATAATCACTAAAATTTTAGTAGGTGCGTTAGCGTTAGCCGTTACCAATGTCTATGCCGCTGAATTGATGACCAAAGCGGAATTTGaaa                                                                                                                                           >  2:749251/1‑149 (MQ=255)
           tCAACCCAATAATGTGGGTGGGTGATAGTGTGATAACAACTCTGGAGCCGTAATATGAAAATAATCTCTAAAATGTTAGTCGGTGCGTTAGCGTTAGCCGTTACCAATGTCTATGCCGCTGAATTGATGACCAAAGCGGAATTTGaaaa                                                                                                                                          >  1:971612/1‑149 (MQ=255)
              aCCCAATAATGTGGGTGGGTGATAGTGTGATAACAACTCTGGAGCCGTAATATGAAAATAATCTCTAAAATGTTAGTCGGTGCGTTAGCGTTAGCCGTTACCAATGTCTATGCCGCTGAATTGATGACCAAAGCGGAATTTGAAAAAGt                                                                                                                                       >  2:534946/1‑149 (MQ=255)
               cccAATAATGTGGGGGCGTGATAGTGTGAACACAACTCGGGAGCCGTAATCTGAAAAAAATCTCTAAACAGTAAGTCGGTGCGGTAGCGTTAGCCGTTACCAATGTCTATGGCGCTGAATTGATGACCAAAGCGGAATTTGAAAAAGtt                                                                                                                                      <  1:584323/149‑1 (MQ=255)
               acaaataatGTGGTTGGGTGATAGTGTGATATCAACTCTGGATGCGTAATATGAAAATAATCTCTAAAATGATAGTCGTTGCGTTAGCGTTAGCCGTTACCAATGTCTATGCCGCTGATTTGATGACCAAAGCTGATTTTGAAAAAGtt                                                                                                                                      <  1:143621/146‑1 (MQ=255)
                ccAATAATGTGGGTGGGTGATAGTGTGATAACAACTCTGGAGCCGTAATATGAAAATAATCTCTAAAATGTTAGTCGGTGCGTTAGCGTTAGCCGTTACCAATGTCTATGCCGCTGAATTGATGACCAAAGCGGAATTTGAAAATGTTg                                                                                                                                     >  2:732752/1‑149 (MQ=255)
                  aataatGTGGGTGGGTGATAGTGTGATAACAACTCTGGAGCCGTAATATGAAAATAATCTCTAAAATGTTAGTCGGTGCGTTAGCGTTAGCCGTTACCAATGTCTATGCCGCTGAATTGATGACCAAAGCGGAATTTGAAAAAGTTGaa                                                                                                                                   <  1:893987/149‑1 (MQ=255)
                    taatGTGGGTGGGTGATAGTGTGATAACAACTCTGGAGCCGTAATATGAAAATAATCTCTAAAATGTTAGTCGGTGCGTTAGCGTTAGCCGTTACCAATGTCTATGCCGCTGAATTGATGACCAAAGCGGAATTTGAAAAAGTTGAATc                                                                                                                                 <  2:390543/149‑1 (MQ=255)
                       tgtgGGTGGGTGATAGTGTGATAACAACTCTGGAGCCGTAATATGAAAATAATCTCTAAAATGTTAGTCGGTGCGTTAGCGTTAGCCGTTACCAATGTCTATGCCGCTGAATTGATGACCAAAGCGGAATTTGAAAAAGTTGAATCGCa                                                                                                                              <  2:151572/149‑1 (MQ=255)
                        gtgggtggGTGATAGTGTGATAACAACTCTGGAGCCGTAATATGAAAATAATCTCTAAAATGTTAGTCGGTGCTTTAGCGTTAGCCGTTACCAATGTCTATGCCGCTGAATTGATGACCAAAGCGGAATTTGAAAAAGTTGAATCGCat                                                                                                                             >  2:309827/1‑148 (MQ=255)
                         ggtgtgtgtgATAGTGTGATAACAACTCTGTCGCCGTAATATGAAAATAATCTCTAAAATCTTCGTCGGTGCGATAGCGTTAGCCCTTACCACTGGCTATGCCGCTGAATTGATGACCAAAGCGGAATTTGAAAAAGTTGAATCGCAga                                                                                                                            <  2:488909/146‑2 (MQ=255)
                          gggtgggtGATAGTGTGATAACAACTCTGGAGCCGTAATATGAAAATAATCTCTAAAATGATAGTCGGTGCGTTAGCGTTAGCCGTTACCAATGTCTATGCCGCTGAATTGATGACCAAAGCGGAATTTGAAAAAGTTGAATCGCAGTa                                                                                                                           <  2:778422/149‑1 (MQ=255)
                            gtgggtgATAGTGTGATAACAACTCTGGAGCCGTAATATGAAAATAATCTCTAAAATGTTAGTCGGTGCGTTAGCGTTAGCCGTTACCAATGTCTATGCCGCTGAATTGATGACCACAGCGGAATTTGAAAAAGTTGAATCGCAGTATg                                                                                                                         <  1:28823/149‑1 (MQ=255)
                             tgggtgATAGTGTGATAACAACTCTGGAGCCGTAATATGAAAATAATCTCTAAAATGTTAGTCGGTGCGTTAGCGTTAGCCGTTACCAATGTCTATGCCGCTGAATTGATGACCAAAGCGGAATTTGAAAAAGTTGAATCGCATTATGa                                                                                                                        >  2:813340/1‑149 (MQ=255)
                                gtgATAGTGTGATAACAACTCTGGAGCCGTAATATGAAAATAATCTCTAAAATGTTAGTCGGTGCGTTAGCGTTAGCCGTTACCAATGTCTATGCCGCTGAATTGATGACCAAAGCGGATTTTGAAAAAGTTGAATCGCAGTATGaaaa                                                                                                                     >  1:929832/1‑149 (MQ=255)
                                 tgATAGTGTGATAACAACTCTGGAGCCGTAATATGAAAATAATCTCTAAAATGTTAGTCGGTGCGTTAGCGTTAGCCGTTACCAATGTCTATGCCGCTGAATTGATGACCAAAGCGGAATTTGAAAAAGTTGAATCGCAGTATGaaaaa                                                                                                                    >  1:272715/1‑149 (MQ=255)
                                      gtgtGATAACAACTCTGGAGCCGTAATATGAAAATAATCTCTAAAATGTTAGTCGGTGCGTTAGCGTTAGCCGTTACCACTGTCTATGCCGCTGAATTGATGACCAAAGCGGAATTTGAAAAAGTTGAATCGCAGTATGAAAAAATAgg                                                                                                               <  2:592016/149‑1 (MQ=255)
                                       tgtgATAACAACTCTGGAGCCGTAATATGAAAATAATCTCTAAAATGTTAGTCGGTGCGTTAGCGTTAGCCGTTACCAATGTCTATGCCGCTGAATTGATGCCCAAAGCGGAATTTGAAAAAGTTGAATCGCAGTATGAAAAAATAGGt                                                                                                              >  2:536463/1‑149 (MQ=255)
                                         tgATAACAACTCTGGAGCCGTAATATGAAAATAATCTCTAAAATGTTAGTCGGTGCGTTAGCGTTAGCCGTTACCAATGTCTATGCCGCTGAATTGATGACCAAAGTGGAATTTGAAAAAGTTGAATCGCAGTATGAAAAAATAGGTGa                                                                                                            >  2:304812/1‑149 (MQ=255)
                                         tgATAACAACTCTGGAGCCGTAATATGAAAATAATCTCTAAAATGTTAGTCGGTGCGTTAGCGTTAGCCGTTACCAATGTCTATGCCGCTGAATTGATGACCAAAGTGGAATTTGAAAAAGTTGAATCGCAGTATGAAAAAATAGGTGa                                                                                                            >  2:854553/1‑149 (MQ=255)
                                         tgATAACAACTCTGGAGCCGTAATATGAAAATAATCTCTAAAATGTTAGTCGGTGCGTTAGCGTTAGCCGTTACCAATGTCTATGCCGCTGAATTGATGACCAAAGCGGAATTTGAAAAAGTTGAATCGCAGTATGAAAAAATAGGTGa                                                                                                            >  2:178150/1‑149 (MQ=255)
                                             aacaacTCTGGAGCCGTAATATGAAAATAATCTCTAAAATGTTAGTCGGTGCGTTAGCGTTAGCCTTTACCAATGTCTATGCCGCTGAATTGATGAACAAAGCGGAATTTGAAAAAGTTGAATCGCAGTATGAAAAAATAGGTGATAtt                                                                                                        <  1:836423/149‑1 (MQ=255)
                                             aacaaaACTGGAGACGTAATATGAAAATAATCTATAAAATGTTAGTCGGTGCTTAAGCGTTAGCCGTTACCAATGTCTATGCCGCTGAATTGATGACCAAAGCGGAATTTGAAAAAGTTGAATCGCAGTATGAAAAAATAGGTGATAtt                                                                                                        <  1:940646/149‑1 (MQ=255)
                                               caacTCTGGAGCCGTAATATGAAAATAATCTCTAAAATGTTAGTCTGTGCGTTAGAGTTAGCCGTTACCAATGTCTATGCCGCTGAAGAGATGACCAAAGCGGAATATGAAAAAGTTGAATCGCAGTATGAAAAAATACGTGATATTTc                                                                                                      >  2:591619/1‑149 (MQ=255)
                                                aacTCTGGAGCCGTAATATGAGAATAATCTCTAAAATGTTAGTCGGTGCGTTAGCGTTAGCCGTTACCAATGTCTATGCCGCTGAATTGATGACCAAAGCGGAATTTGAAAAAGTTGAATCGCAGTATGAAAAAATAGGTGATATTTCa                                                                                                     >  1:279402/1‑149 (MQ=255)
                                                aacTCTGGAGCCGTAATATGAAAATAATCTCTAAAATGTTAGTCGGTGCGTTAGCGTTAGCCGTTACCAATGTCTATGCCGCTGAATTGATGACCAAAGCGGAATTTGAAAAAGTTGAATCGCAGTATGAAAAAATAGGTGATATTTCa                                                                                                     >  1:506547/1‑149 (MQ=255)
                                                aacTCTGGAGCCGTAATATGAAAATAATCTCTAAAATGTTACTCGGTGCGTTAGCGATAGCAGTTACCAATGTCAATGCCGCTGAATTGATGACCAAATCGGAATTTGAAAAAGTTGAATCGCAGTATGAAAAAATAGGTGATATTTCa                                                                                                     >  2:638253/1‑149 (MQ=255)
                                                 acTCTGGAGCCGTAATATGAAAATAATCTCTAAAATGTTAGTCGGTGCGTTAGCGTTAGCCGTTACCAATGTCTATGCCGCTGAATTGATGACCAAAGCGGAATTTGAAAAAGTTGAATCGCAGTATGAAAAAATAGGTGATATTTCaa                                                                                                    <  2:783077/149‑1 (MQ=255)
                                                 acTCTGGAGCCGTAATATGAAAATAATCTCTAAAATGTTAGTCGGTGCGTTAGCGTTAGCCGTTACCAATGTCTATGCCGCTGAATTGATGACCAAAGCGGAATTTGAAAAAGTTGAATCGCAGTATGAAAAAATAGGTGATATTTCaa                                                                                                    <  1:875894/149‑1 (MQ=255)
                                                   tctGGAGCCGTAATATGAAAATAATCTCTAAAATGTTAGTCGGTGCGTTAGCGTTAGCCGTTACCAATGTCTATGCCGCTGAATTGATGACCAAATCGGAAATTGAAAAAGTTGAATCGCAGTATGAAAAAATAGGTGATATTTCAAcc                                                                                                  >  2:854209/1‑149 (MQ=255)
                                                            gTAATATGAAAATAATCTCTAAAATGTTAGTCGGTGCGTTAGCGTTAGCCGTTACCAATGTCTATGCCGCTGAATTGATGACCAAAGCGGAATTTGAAAAAGTTGAATCGCAGTATGAAAAAATAGGTGATATTTCAACCAGCAATGaa                                                                                         <  1:475179/149‑1 (MQ=255)
                                                              aaTATGAAAATAATCTCTAAAATGTTAGTCGGTGCGTTAGCGTTTGCCGTAACCAATGTCTATGCCGCTGAATTGATGACCAAAGCGGAATTTGAAAAAGTTGAATCGCAGTATGAAAAAATAGGTGATATTTCAACCAGCAATGAAAt                                                                                       <  1:579496/149‑1 (MQ=255)
                                                              aaTATGAAAATAATCTCTAAAATGTTAGTCGGTGCGTTAGCGTTAGCCGTTACCAATGTCTATGCCGCTGAATTGATGACCAAAGCGGAATTTGAAAAAGTTGAATCGCAGTATGAAAAAATAGGTGATATTTCAACCAGCAATGAAAt                                                                                       >  2:332818/1‑149 (MQ=255)
                                                                   gAAAATAATCTCTAAAATGTTAGTCGGTGCGTTAGCGTTAGCCGTTACCAATGTCTATGCCGCTGAATTGATGACCAAAGCGGAATTTGAAAAAGTTGAATCGCAGTATGAAAAAATAGGTGATATTTCAACCAGCAATGAAATGTCGa                                                                                  <  2:494540/149‑1 (MQ=255)
                                                                   gAAAATAATCTCTAAAATGTTAGTCGGTGCGTTAGCGTTAGCCGTTACCAATGTCTATGCCGCTGAATTGATGACCAAAGCGGAATTTGAAAAAGTTGAATCGCAGTATGAAAAAATAGGTGATATTTCAACCAGCAATGAAATGTCGa                                                                                  >  1:922053/1‑149 (MQ=255)
                                                                    aaaaTAATCTCTAAAATGTTAGTCGGTGCGTTAGCGTTAGCCGTTACCAATGTCTATGCCGCTGAATTGATGACCAAAGCGGAATTTGAAAAAGTTGAATCGCAGTATGAAAAAATAGGTGATATTTCAACCAGCAATGAAATGTCGAc                                                                                 >  1:632920/1‑149 (MQ=255)
                                                                        taatCTCAAAAATGTAAGTCGGTGCCTTAGCGTTAGCCGTTACCAATGTCTATGCCGCTGAATTGATGACCACAGCGGAATTTGAAAAAGTTGAATCGCACTATGAAAAAATAGGTGAAATTGCAACCCGCAATGAAATGTCGACTGCa                                                                             <  2:213512/149‑1 (MQ=255)
                                                                            ctctAAAATGTTAGTCGGTGCGTTAGCGTTAGCCGTTACCAATGTCTATGCCGCTGAATTGATGACCAAAGTGGAATTTGAAAAAGTTGAATCGCAGTATGAAAAAATAGGTGATATTTCAACCAGCAATGAAATGTCGACTGCAGATg                                                                         <  1:854553/149‑1 (MQ=255)
                                                                            ctctAAAATGTTAGTCGGTGCGTTAGCGTTAGCCGTTACCAATGTCTATGCCGCTGAATTGATGACCAAAGTGGAATTTGAAAAAGTTGAATCGCAGTATGAAAAAATAGGTGATATTTCAACCAGCAATGAAATGTCGACTGCAGATg                                                                         <  1:304812/149‑1 (MQ=255)
                                                                             tctAAAATGTTAGTCGGTGCGTTAGCGTTAGCCGTTACCAATGTCTATGCCGCTGAATTGATGACCAAAGCGGAATTTGAAAAAGTTGAATCGCAGTATGAAAAAATAGGTGATATTTCATCCAGCAATGAAATGTCGACTGCAGATGc                                                                        >  1:140719/1‑149 (MQ=255)
                                                                             tctAAAATGTTAGTCGGTGCGTTAGCGTTAGCCGTTACCAATGTCTATGCCGCTGAATTGATGACCAAAGCGGAATTTGAAAAAGTTGAATCGCAGTATGAAAAAATAGGTGATATTTCAACCAGCAATGAAATGTCGACTGCAGATGc                                                                        <  2:971612/149‑1 (MQ=255)
                                                                              ctAAAATGTTAGTCGGTGCGTTAGCGTTAGCCGTTACCAATGTCTATGCCGCTTAATTGATGACCAAAGCGGAATTTGAAAAAGTTTAATCGCAGTATGAAAAAAGAGGTGATATTTCACCCAGCAATGAAATGTCGACTGCAGAAGCa                                                                       >  1:682653/1‑149 (MQ=255)
                                                                              ctAAAATGTTAGTCGGTGCGTTAGCGTTAGCCGGTACCAATGTCTATGCCGCTCAATTGATGACCAAAGCGGAATTTGAAAAAGTTGAATCGCAGTATGAAAAAATAGGTGATAATTCAACCAGCAATGAAATTTCGACTGAACATGCa                                                                       >  1:617310/1‑149 (MQ=255)
                                                                               aaaaaTGTTAGTCGGTGCGTAAGCGATAGCCGTTACCAATGTCTATGCCGCTGAATTGATGACCAAAGCGGAATTTGAAAAAGTTGAATCGCAGTATGAAAAAATAGGTGATATTTCAACCAGCAATGAAATGTCGACTGCAGATGCaa                                                                      <  1:553618/148‑1 (MQ=255)
                                                                                       tAGTCGGTGCGTTAGCGTTAGCCGTTACCAATGTCTATGCCGCTGAATTGATGACCAAAGCGGAATTTGAAAAAGTTGAATCGCAGTATGAAAAAATAGGTGATATTTCAACCAGCAATGAAATGTCGACTGCAGATGCAAAAGAAGAt                                                              >  1:835620/1‑149 (MQ=255)
                                                                                       tAGTCGGTGCGTTAGCGTTAGCCGTTACCAATGTCTATGCCGCTGAATTGATGACCAAAGCGGAATTTGAAAAAGTTGAATCGCAGTATGAAAAAATAGGTGATATTTCAACCAGCAATGAAATGTCGACTGCAGATGCAAAAGAAGAt                                                              <  1:457117/149‑1 (MQ=255)
                                                                                         gTCGGTGCGTTAGCGTTAGCCGTTACCAATGTCTATGCCGCTGAATTGATGACCAAAGCGGAATTTGAAAAAGTTTAATCGGAGTATGAAAAAATAGGTGATATTACAACCAGCTATGAAATGTCGACTGCAGATGCAAAAGAAGAttt                                                            >  1:377303/1‑149 (MQ=255)
                                                                                                cgttagcgttagCCGTTACCAATGTCTATGCCGCTTAATTTATGACCAAAGCGGAATTTGAAAAATTTGAATCGCAGTATGAAAAAATAGGTGATATTTCAACCAGCAATGAAATGTCGACTGCAGATGCAAAAGAAGATTTGATCaaa                                                     <  1:496801/149‑1 (MQ=255)
                                                                                                      cgttagcCGTTACCAATGTCTATGCCGCTGAATTGATGACCAAAGCGGAATTTGAAAAAGTTGAATCGCAGTATGAAAAAATAGGTGATATTTCAACCAGCAATGAAATGTCGACTGCAGAGGCTAAAGAAGATTTGATCAAAAAAGCg                                               >  2:900079/1‑149 (MQ=255)
                                                                                                        ttagcCGTTACCAATGTCTATGCCGCTGAATTGATGACCAAAGCGGAATTTGATAAAGTTGAATCGCAGTATGAAAAAATAGGTGATATTTCAACCAGCAATGAAATGTAGACTCAAGATGCAAAAGTAGATTTGATCAAAAAAGCCGa                                             >  1:665973/1‑149 (MQ=255)
                                                                                                        ttagcCGTTACCAATGTCTATGCCGCTGAATTGATGACCAAAGCGGAATTTGAAAAAGTTGAATCGCAGTATGAAAAAATAGGTGATATTTCAACCAGCAATGAAATGTCGACTGCAGATGCAAAAGAAGATTTGATCAAAAAAGCGGa                                             >  1:310624/1‑149 (MQ=255)
                                                                                                        ttagcCGTTACCAATGTCTATGCCGCTGAATTGATGACCAAAGCGGAATTTGAAAAAGTTGAATCGCAGTATGAAAAAATAGGTGATATTTCAACCAGCAATGAAATGTCGACTGCAGATGCAAAAGAAGATTTGATCAAAAAAGCGGa                                             >  1:634359/1‑149 (MQ=255)
                                                                                                            ccGTTACCAATGTCTATGCCGCTGAATTGAAGACCAAAGAGGAAGTTGAAAAAGTTGAATCGCAGTATGAAAAAATAGGTGATATTTCAACCAGCAATGAAATGACGACTGCACATGCAAAAGAAGATTTGATCAAAAAAGCGGATCaa                                         >  1:234224/1‑149 (MQ=255)
                                                                                                                      tGTCTATGCCGCTGAATTGATGACCAAAGCGGAATTTGAAAAAGTTGAATCGCAGTATGAAAAAATAGGTGATATTTCAACCAGGAATGAAATGTGGACTGCAGATGCTAAAGAAGATTTGATCAAAAAAGCGGATGAAAAAGGGGCTg                               <  1:749251/149‑1 (MQ=255)
                                                                                                                        tCTATGCCGCTGAATTGATGACCAAAGCGGAATTTGAAAAAGTTGAATCGCAGTATGAAAAAATAGGTGATATTTCAACCAGCAATGAAATGTCGACTGCAGATGCAAAAGAAGATTTGATCAAAAAAGCGGATGAAAAAGGGGCTGAt                             <  2:929832/149‑1 (MQ=255)
                                                                                                                         cTATGCCGCTGAATTGATGACCAAAGCGGAATTTGAAAAAGTTGAATCGCAGTATGAAAAAATAGGTGATATTTCAACCAGCAATGAAATGTCGACTGCAGATGCAAAAGAAGATTTGATCAAAAAAGCGGATGAAAAAGGGGCTGAtg                            <  2:279402/149‑1 (MQ=255)
                                                                                                                            tgcagatGAATTGATGACCAAATCTGAATTTCAAAAAGTTGAATCGCAGTATGAAAAAATAGCTGATATTTCAACCAGCAATGAAATGTCGACTGGAGATGCAAAAGAAGATTTCATCAAAAAAGCGGATGAAAAAGGGGCTGATgtgt                         <  1:329319/143‑1 (MQ=255)
                                                                                                                              cctcTGAATTGATTACCAATCCGTAATTTGAAAAATTTGAATCGCAGTATTAAAAAATAGGTGATATTTCTACCAGCAATGAAATGTCGACTGCAGATGCAAAAGAAGATTTGATCAAAAAAGCGGATGAAATAGGGGCTGATGTGTTg                       <  1:293408/146‑1 (MQ=255)
                                                                                                                              ccGCTGAATTGATGTCCAATGCGGATTTTGAAAAATGTGAATCGCATTATGAAAAACTAGGGGATATTTCACCCCGCACTGAAATGTCGACTGAAGATGCAAAAGAAGATTTGATCAAAAAAGCGGATGAAACAGGGGCTGATgtgtgg                       <  2:900984/149‑3 (MQ=255)
                                                                                                                              ccGCTGAATTGATGACCAAAGCGGAATTTGAAAAAGTTGGATCTCAGTATGAAAAAATAGGTGATATTTCAACCAGCAATGAAATGTCGACTGCAGATGCAAAAGAAGATTTGATCAAAAAAGCGTATGAAAAAGGTGCTGATGTGTTg                       >  1:212094/1‑149 (MQ=255)
                                                                                                                               cGCTGAATTGATGACCAAAGCGGAATTTGAAAAAGTTGAATCGCAGTATGAAAAAATAGGTGATATTTCAACCAGCAATGAAATGTCGACTGCAGATGCAAAAGAAGATTTGATCAAAAAAGCGGATGAAAAAGGGGCTGATGTGTTgg                      >  2:717335/1‑149 (MQ=255)
                                                                                                                               cGCTGAATTGATGACCAAAGCGGAATTTGAAAAAGTTGAATCGCAGTATGAAAAAATAGGTGATATTTCAACCAGCAATGAAATGTCGACTGCAGATGCAAAAGAAGATTTGATCAAAAAAGCGGATGAAAAAGGGGCTGATGTGTTgg                      >  2:821894/1‑149 (MQ=255)
                                                                                                                                    aaTTGATGACCAAAGCGGAATTTGAAAAAGTTGAATCGCAGTATGAAAAAATAGGTCATATTTCAACCAGCAATGAAATGTCGACTGCAGATGCAAAAGAAGATTTGATAAAAAAAGCGGATGAAAAAGGGGCTGATGTGTTGGTACTg                 <  1:813340/149‑1 (MQ=255)
                                                                                                                                       tgatgaCCAAAGCGGAATTTGAAAAAGTTGAATCGCAGTATGAAAAAATAGGTGATATTTCAACCAGCAATGAAATTTCGACTGCAGATGCAAAAGAAGATTTGATCAAAAAAGCGGATCAAAAAGGGGCTGATGTGTTTGTACTGAca              >  1:429960/1‑148 (MQ=255)
                                                                                                                                         atgaCCAAAGCGGATTTTGAAAAAGTTGAATCGCAGTATGAAAATATAGGTGATATTTCAACCAGCAATGAAATGTCGACTGCAGATGCAAAAGAAGATTTGATAAAAAAAGCGGATGAAAAAGGGGCTGATGTGTTGGTACTGACCTc            >  2:553312/1‑149 (MQ=255)
                                                                                                                                           gtccAACGCGGATTTTGAAAAATTTGAATCGCACTATGAAAAAATAGGTGATATTTCAACCAGCAATGAACTGTCGACTGCAGATGCAAAAGAAGATTTGATCAAAAAAGCGGATGAACAAGGGGCGGATGTGTTGGTACTGACCTCCg          <  2:447428/147‑1 (MQ=255)
                                                                                                                                           gaCCAAAGCGGAATTTGAAAAAGTTTAATCGCAGTATGAAAAAATAGGTGATATTTCAACCAGCAATGAAATGTCGACTGCAGATGCAAAAGAAGGTTTGATCAAAAAAGCGGATGAAAAAGGGGCTGATGTGTTGGTACTGACCTCCg          <  1:534946/149‑1 (MQ=255)
                                                                                                                                           gaCCAAAGCGGAATTTGAAAAAGTTGAATCGCAGTATGAAAAAATAGGTGATATTTCAACCAGCAATGAAATGTCGACTGCAGATGCAAAAGAAGATTTGATCAAAAAAGCGGATGAAAAAGGGGCTGATGTGTTGGTACTGACCTCCg          >  1:574235/1‑149 (MQ=255)
                                                                                                                                            aCCAAAGCGGAATTTGAAAAAGTTGAATCGCAGTATGAAAAAATAGGTGATATTTCAACCAGCAATGAAATGTCGACTGCAGATGCAAAAGAAGATTTGATCAAAAAAGCGGATGAAAAAGGGGCTTATGTGTTGGTACTGACCTCCgg         >  1:862184/1‑149 (MQ=255)
                                                                                                                                              cAAAGCGGAATTTGAAAAAGTTGAATCGCAGTATGAAAAAATAAGTGATATTTCAACCAGCAATGAAATGTCGACTGCAGATGCAAAAGAAGATTTGATCAAAAAAGCGGATGAAAAAGGGTCTGATGTGTTGGTACTGACCTCCGGta       >  2:736852/1‑148 (MQ=255)
                                                                                                                                               aaaGCGGAATTTGAAAAAGTTGAATCGCAGTATGAAAAAATAGGTGATATTTCAACCAGCAATGAAATGTCGACTGCAGATGCAAAAGAAGATTTGATCAAAAAAGCGGATGAAAAAGGGGCTGATGTGTTGGTACTGACCTCCGGTCa      >  1:71965/1‑149 (MQ=255)
                                                                                                                                                aaGCGGAATTTGAAAAAGTTGAATCGCAGTATCAAAAAATAGGTGATATTTCAACCAGCAATGAAATGTCGACTGCAGATGCAAAAGAAGATTTGAACAAAAAAGCGGAAGAAAAAGGGACTGATTTCTTGGTACTGAACACCGGTCaa     >  2:997309/1‑149 (MQ=255)
                                                                                                                                                  gCGGAATTTGAAAATGTTGAATCGCAGTATGAAAAAATAGGTGATATTTCAACCAGCAATGAAATGTCGACTGCAGATGCAAAAGAAGATTTGATCAAAAAAGCGGATGAAAAAGGGGCTGATGTGTTGGTACTGACCTCCGGTCAAAc   <  1:732752/149‑1 (MQ=255)
                                                                                                                                                   cGGAATTTGAAAAAGTTGAATCGCAGTATGAAAAAATAGGTGATATTTCAACCAGCAATGAAATGTCGACTGCAGATGCAAAAGAAGATTTGATCAATAAAGCGGATGAAAAAGGGGCTGATGTGTTGGTACTGACCTCCGGTCAAACt  >  1:562696/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
CTAATTATTAATCAACCCAATAATGTGGGTGGGTGATAGTGTGATAACAACTCTGGAGCCGTAATATGAAAATAATCTCTAAAATGTTAGTCGGTGCGTTAGCGTTAGCCGTTACCAATGTCTATGCCGCTGAATTGATGACCAAAGCGGAATTTGAAAAAGTTGAATCGCAGTATGAAAAAATAGGTGATATTTCAACCAGCAATGAAATGTCGACTGCAGATGCAAAAGAAGATTTGATCAAAAAAGCGGATGAAAAAGGGGCTGATGTGTTGGTACTGACCTCCGGTCAAACT  >  NC_000913/346419‑346714

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: