Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,437,910 A→T 7.0% intergenic (+16/‑114) trkA → / → mscL NAD‑binding component of TrK potassium transporter/mechanosensitive channel protein, high conductance

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,437,9100AT 7.0% 300.2 / 6.6 114intergenic (+16/‑114)trkA/mscLNAD‑binding component of TrK potassium transporter/mechanosensitive channel protein, high conductance
Reads supporting (aligned to +/- strand):  ref base A (42/61);  new base T (4/5);  total (46/68)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 117 total aligned reads displayed.

TGGAAACGACGTGATGATTGCCAATGACAATCTGCGCATTGAGCAAGGCGATCACGTAATTATGTTCCTCACAGATAAAAAGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGTTAGACTTATGGTTGTCGGCTTCATAGGGAGAATAACATGAGCATTATTAAAGAATTTCGCGAATTTGCGAT  >  NC_000913/3437762‑3438058
                                                                                                                                                    |                                                                                                                                                    
tGGAAACGACGTGATGATTGCCAATGACAATCTGCGCATTGAGCAAGGCGATCACGTAATTATGTTCCTCACAGATAAAAAGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTaa                                                                                                                                                      >  2:980472/1‑149 (MQ=255)
  gAAACGAGGTGATGATTGCCAATGACAATCTGCGCAGTGAGCAAGGAGATCACGTAATTATGTTCCTCACAGATAAAAAGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTGCTTCTTGTAATTAATAAGGCGTCTAATg                                                                                                                                                    >  2:723823/1‑149 (MQ=255)
   aaaCGACGTGATGATTGCCAATGACAATCTGCGCATTGAGCAAGGCGATCACGTAATTATGTTCCTCACAGATAAAAAGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGa                                                                                                                                                   <  1:109919/149‑1 (MQ=255)
            gatgatTGCCAATGACAATCTGCGCATTGAGCAAGGCGATCACGTAATTATGTTCCTCACAGATAATAAGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCttatt                                                                                                                                          <  2:115054/149‑1 (MQ=255)
            gatgatTGCCAATGACAATCTGCGCATTGAGCAAGGCGATCACGTAATTATGTTCCTCACAGATAAAAAGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCttatt                                                                                                                                          >  1:3169/1‑149 (MQ=255)
            gatgatTGCCAATGACAATCTGCGCATTGAGCAAGGCGATCACGTAATTATGTTCCTCACAGATAAAAAGTTTATAACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCttatt                                                                                                                                          >  1:876263/1‑149 (MQ=255)
             atgatTGCCAATGACAATCTGCGCATTGAGCAAGGCGATCACGTAATTATGTTCCTCACAGATAAAAAGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCttatta                                                                                                                                         <  2:608848/149‑1 (MQ=255)
              tgatTGCCAATGACAATCTGCGCATTGAGCAAGGCGATCACGTAATTATGTTCCTCACAGATAAAAAGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCATTAGACGCCttattaa                                                                                                                                        <  2:882971/149‑2 (MQ=255)
               gatTGCCAATGACAATCTGCGCATTGAGCAAGGCGATCACGTAATTATGTTCCTCACAGATAAAAAGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTattatt                                                                                                                                       >  2:27715/1‑149 (MQ=255)
                atTGCCAATGACAATCTGCGCATTGAGCAAGGCGATCACGTAATTATGTTCCTCACAGATAAAAAGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTAttt                                                                                                                                      >  2:981039/1‑149 (MQ=255)
                atTGCCAATGACAATCTGCGCATTGAGCAAGGCGATCACGTAATTATGTTCCTCACAGATAAAAAGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTAttt                                                                                                                                      >  2:637481/1‑149 (MQ=255)
                atTGCCAATGACAATCTGCGCATTGAGCAAGGCGATCACGTAATTATGTTCCTCACAGATAAAAAGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTAttt                                                                                                                                      >  1:742984/1‑149 (MQ=255)
                 ttGCCAATGACAATCTGCGCATTGAGCAAGTCGATCACGTAATTATGTTCCTCACAGATAAAAAGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGAGGCCTTATTATTTc                                                                                                                                     >  1:325966/1‑149 (MQ=255)
                 ttGCCAATGACAATCTGCGCATTGAGCAAGGCGATCACGTAATTATGTTCCTCACAGATAAAAAGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCATTAGACGCCTTATTAatta                                                                                                                                     <  1:1007968/149‑2 (MQ=255)
                 ttGCCAATGACAATCTGCGCATTGAGCAAGGCGATCACGTAATTATGTTCCTCACAGATAAAAAGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCATTCTTCTTGTAATTAATAAGGCGTCATTAGACGCCTTATTAatta                                                                                                                                     <  1:167076/149‑2 (MQ=255)
                 ttGCCAATGACAATCTGCGCATTGAGCAAGGCGATCACGTAATTATGTACCTCACAGATAAAAAGTTTATTACAGACGTCCAAAGACTCTTCCATCAAAGCCATTTATTCTTGTAATTAATAATGCGAATAAATACGCCatataaatta                                                                                                                                     >  2:98537/1‑143 (MQ=255)
                   gCCAATGACAATCTGCGCATTGAGCAAGGCGATCACGTAATTATGTTCCTCACAGATAAAAAGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTccc                                                                                                                                   <  1:459142/149‑1 (MQ=255)
                    ccAATGACAATCTGCGCATTGAGCAAGGCGATCACGTAATTATGTTCCTCACAGATAAAAAGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCt                                                                                                                                  >  1:96825/1‑149 (MQ=255)
                    ccAATGACAATCTGCGCATTGAGCAAGGCGATCACGTAATTATGTTCCTCACAGATAAAAAGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGGCATTAGACGCCTTATTAattacaa                                                                                                                                  <  1:837338/149‑5 (MQ=255)
                    ccAATGACAATCTGCGCATTGAGCAAGGCGATCACGTAATTATGTTCCTAACAGATAAAAAGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAAAGACGCCTTATTATTTCCCt                                                                                                                                  <  2:314138/149‑1 (MQ=255)
                     cAATGACAATCTGCGCATTGAGCAAGGCGATCACGTAATTATGTTCCTCACAGATAAAAAGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCtt                                                                                                                                 <  1:637481/149‑1 (MQ=255)
                      aaTGACAATCTGCGCATTGAGCAAGGCGATCACGTAATTATGTTCCTCACAGATAAAAAGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCttt                                                                                                                                <  2:337570/149‑1 (MQ=255)
                       aTGACAATCTGCGCATTGAGCAAGGCGATCACGTAATTATGTTCCTCACAGATAAAAAGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTg                                                                                                                               >  2:124574/1‑149 (MQ=255)
                        tGACAATCTGCGCATTGAGCAAGGCGATCACGTAATTATGTTCCTCACAGATAAAAAGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGa                                                                                                                              <  1:642348/149‑1 (MQ=255)
                         gACAATCTGCGCATTGAGCAAGGCGATCACGTAATAATGTTCCTCACAGATAAAAAGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGAt                                                                                                                             <  1:975508/149‑1 (MQ=255)
                           cAATCTGCGCATTGAGCAAGGCGATCACGTAATTATGTTCCTCACAGATAAAAAGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCGTTCTTCTAGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTa                                                                                                                           >  2:428921/1‑149 (MQ=255)
                            aaTCTGCGCATTGAGCAAGGCGATCACGTAATTATGTTCCTCACAGATAAAAAGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTAt                                                                                                                          <  1:127783/149‑1 (MQ=255)
                              tCTGCGCATTGAGCAAGGCGATCACGTAATTATGTTCCTCACAGATAAAAAGTTTATTAACTACGTCGAAAGACTCTTCCAGCCAAGCCCGTTCTTCTTGTAATTAATAAGGCGTCTAATGACTCCTTATTATTTCCCTTTGATTATCa                                                                                                                        >  2:926680/1‑149 (MQ=255)
                               cTGCGCATTGAGCAAGGCGATCACGTAATTATGTTCCTCACAGATAAAAAGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCaa                                                                                                                       <  2:319441/149‑1 (MQ=255)
                                   gcATTCAGCAAGGCGATCACGTAATTATGTTCCTGACAGATAAAAAGTTTATTACAGACGTAGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGat                                                                                                                   <  1:85448/149‑1 (MQ=255)
                                     aTTGAGCAAGGCGATCACGTAATTATGTTCCTCACAGATAAAAAGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGatta                                                                                                                 >  1:871357/1‑149 (MQ=255)
                                       tGAGCAAGGCGATCACGTAATTATGTTCCTCACAGATATAAAGTTTATTACCGACGTCGAAAGACTCTTCCATCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGattaat                                                                                                               <  1:99558/149‑1 (MQ=255)
                                           cAAGGCGATCACGTAATTATGTTCCTCACAGATAAAAAGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTaaa                                                                                                           <  2:234192/149‑1 (MQ=255)
                                           cAAGGCGATCACGTAATTATGTTCCTCACAGATAAAAAGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTaaa                                                                                                           <  2:471085/149‑1 (MQ=255)
                                           cAAGGCGATCACGTAATTATGTTCCTCACAGATAAAAAGTTTATTACCGACGTCGAAAGACTCTTCCAGCAAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTaaa                                                                                                           <  1:425241/149‑1 (MQ=255)
                                                 gctCACGTAATTATGTTCCTCACAGATAAAAAGTTTATTACCGACGTCGAAAGACGCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGACTTATTATTTCCCTTTGATTATCAAGGATTAATTTAattcatt                                                                                                     >  2:352942/3‑149 (MQ=255)
                                                      cGTAATTATGTTCCTCACAGATAAAAAGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTgg                                                                                                >  2:739969/1‑149 (MQ=255)
                                                      cGTAATTATGTTCCTCACAGATAAAAAGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTgg                                                                                                >  2:736711/1‑149 (MQ=255)
                                                        tatttATTTTCATCACAGATAAAAAGTTTATTACAGACGTCGAAAGACTCTTCCAGCCATGACCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACTCCTTAATATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCa                                                                                              <  2:64446/146‑1 (MQ=255)
                                                        tAATTATGTTCCTCACAGATAAAAAGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCa                                                                                              <  1:499409/149‑1 (MQ=255)
                                                                 tCCTCACAGATAAAAAGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATgg                                                                                     <  2:713218/149‑1 (MQ=255)
                                                                  ccTCACAGATAAAAAGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGc                                                                                    <  1:147918/149‑1 (MQ=255)
                                                                      acaGATAAAAAGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTATTGACGCCTTATTATTTCCCTTTGATTATAAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGTCTTaa                                                                                >  1:307447/1‑149 (MQ=255)
                                                                      acaGATAAAAAGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATAAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTaa                                                                                >  2:143451/1‑149 (MQ=255)
                                                                       caGATAAAAAGTTTATTACCGACTTCGAAATACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAAc                                                                               <  1:226310/149‑1 (MQ=255)
                                                                        aGATACAAAGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCATGTAATTAATAATGCGACTAATGACGCCTTATTATTTACCTTTGATTATCAACGATTAATAAAATTCATTCCTGGCAGCAAAATGGCTTAACa                                                                              <  2:469105/149‑1 (MQ=255)
                                                                        aGATAAAAAGTTTATTACCGACGTCGAAAGACTCTTCCAGCCACGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATCTCGCCTTATTATTTCCCTTTGTTTATCAAGGATTATTTAAATTCATTCCTGGCAGGAAATTGGCGTAACa                                                                              >  1:682063/1‑149 (MQ=255)
                                                                        aGATAAAAAGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTACCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACa                                                                              <  2:3169/149‑1 (MQ=255)
                                                                         taTAAAAAGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGACCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACAt                                                                             <  2:482067/148‑1 (MQ=255)
                                                                           tAAAAAGTATAATACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCATCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTAACAAGCATTAATTAAATTCAAACATGGCAGGAAATTGGCTTAAAAttt                                                                           >  2:432786/1‑149 (MQ=255)
                                                                             aaaaGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGt                                                                         <  1:848576/149‑1 (MQ=255)
                                                                             aaaaGTTTATTACAGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTTATAAGGCGTCTAATGACGCCTTATGTTTTGCCTTTGATTATCTAGGATTAATTAAATTCATTACTGGCAGGAAAATGGCTTAACATTTgg                                                                         >  2:498568/1‑148 (MQ=255)
                                                                              aaaGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGtt                                                                        <  1:121460/149‑1 (MQ=255)
                                                                               aaGTTTATTTCCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTGTTGTAATTAATAATGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGTTa                                                                       <  1:510527/149‑1 (MQ=255)
                                                                                 gTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGTTAGa                                                                     <  1:211029/149‑1 (MQ=255)
                                                                                 gTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGTTAGa                                                                     <  1:498739/149‑1 (MQ=255)
                                                                                    tattaCCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATACGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATGAAGGATTAATTTAATTAATTCGTGGGAGGTAAATGGCTTTTCATTTTTTAGACtt                                                                  >  2:919986/1‑149 (MQ=255)
                                                                                    tattaCCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGTTAGACtt                                                                  >  2:923846/1‑149 (MQ=255)
                                                                                    tattaCCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGTTAGACtt                                                                  <  1:408306/149‑1 (MQ=255)
                                                                                        aCCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGTTAGACTTATgg                                                              <  1:810409/149‑1 (MQ=255)
                                                                                                 gAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTAATTCCTGGCAGTAAAATGGCTTAACATTTGTTAGACTTATGGTTGTCGGCt                                                     >  1:1019041/1‑149 (MQ=255)
                                                                                                  aaaGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGTTAGACTTATGGTTGTCGGCtt                                                    >  1:775159/1‑149 (MQ=255)
                                                                                                    tgACTCTTCCAGCCAATCCCTTTCTTCTTGTTATTAATAAGGCGTATAATGACGCCTTATTATTTCCCTTTGTTTATCAAGGATTAATTAAATTCATTGCTGGCAGGAAAATGGCTTAACATTTGTTAGACTTATGGTTGTCGTCTTCa                                                  <  1:98537/148‑1 (MQ=255)
                                                                                                     taCTCTTACAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGTTAGACTTATGGTTGTCGGCTTCAt                                                 <  2:208869/148‑1 (MQ=255)
                                                                                                     gACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGTTAGACTTATGGTTGTCGGCTTCAt                                                 <  1:587100/149‑1 (MQ=255)
                                                                                                      aCTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGTTAGACTTATGGTTGTCGGCTTCATa                                                >  1:1014849/1‑149 (MQ=255)
                                                                                                       atctTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGTTAGACTTATGGTTGTCGGCTTCATAg                                               <  2:793808/148‑1 (MQ=255)
                                                                                                        tctTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGTTAGACTTATGGTTGTCGGCTTCATAgg                                              <  2:876263/149‑1 (MQ=255)
                                                                                                        tctTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGTTAGACTTATGGTTGTCGGCTTCATAgg                                              >  1:1009966/1‑149 (MQ=255)
                                                                                                         ctTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGTTAGACTTATGGTTGTCGGCTTCATAggg                                             <  1:39172/149‑1 (MQ=255)
                                                                                                         ctTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGTTAGACTTATGGTTGTCGGCTTCATAggg                                             <  1:42872/149‑1 (MQ=255)
                                                                                                         ctTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGTTAGACTTATGGTTGTCGGCTTCATAggg                                             <  1:798739/149‑1 (MQ=255)
                                                                                                         ctTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGTTAGACTTATGGTTGTCGGCTTCATAggg                                             <  1:933067/149‑1 (MQ=255)
                                                                                                          ttCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGTTAGACTTATGGTTGTCGGATTCATAGGga                                            >  1:746934/1‑149 (MQ=255)
                                                                                                          ttCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAAAATTTGTTAGACTTATGGTTGTCGGCTTCATAGGga                                            >  2:18924/1‑149 (MQ=255)
                                                                                                           tCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTTATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGTTAGACTTATGGTTGTCGGCTTCATAGGgag                                           >  2:699856/1‑149 (MQ=255)
                                                                                                                 cAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGTTAGACTTATGGTTGTCGGCTTCATAGGGAGAATAAc                                     <  1:629043/149‑1 (MQ=255)
                                                                                                                   aGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAATGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGTTAGACTTATGGTGGTCGGCTTCATAGGGAGAATAACAt                                   <  2:717334/149‑1 (MQ=255)
                                                                                                                   aGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGTTAGACTTATGGTTGTCGGCTTCATAGGGAGAATAACAt                                   <  1:981039/149‑1 (MQ=255)
                                                                                                                    gCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGTTAGACTTATGGTTGTCGGCTTAATAGGGAGAATAACAtt                                  >  2:59394/1‑148 (MQ=255)
                                                                                                                     cccTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGTTAGACTTATGGTTGTCGGCTTCATAGGGAGAATAACATGa                                 <  1:861765/149‑1 (MQ=255)
                                                                                                                       cTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGTTAGACTTATGGTTGTCGGCTTCATAGGGAGAATAACATGAGc                               <  1:739969/149‑1 (MQ=255)
                                                                                                                        tttCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATGTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGTTAGACTTATGGTTGTCGGCTTCATAGGGAGAATAACATGAGCa                              >  1:563308/1‑149 (MQ=255)
                                                                                                                              attGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATTGCTTAACATTTGTTAGACTTATGGTTGTCGGCTTCATTGGGAGAATAACATGAGCAttatta                        <  1:27061/148‑1 (MQ=255)
                                                                                                                                tGTAATTAATTAGTCGTCTAATGTCGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGTAAATGGCTTAACCTTTGTTAGACTTATGGTTGGCGGCGTCATAGGGAGAATAACATGAGCATTATTaaa                      <  1:664659/149‑1 (MQ=255)
                                                                                                                                tGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGTTAGACTTATGGTTGTCGGCTTCATAGGGAGAATAACATGAGCATTATTaaa                      <  2:187984/149‑1 (MQ=255)
                                                                                                                                    attaatAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGTTAGACTTATGGTTGTCGGCTTCATAGGGAGAATAACATGAGCATTATTAAAGAAt                  >  1:238489/1‑149 (MQ=255)
                                                                                                                                       aataaGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGTTAGACTTTTGGTTGTCGGCTTCATAGGGAGAATAACATGAGCATTATTAAAGAATTTc               >  1:347834/1‑149 (MQ=255)
                                                                                                                                       aataaGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGTTAGACTTATGGTTGTCGGCTTCATAGGGAGAATAACATGAGCATTATTAAAGAATTTc               <  1:973865/149‑1 (MQ=255)
                                                                                                                                       aataaGGCGTCATTAGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAAGTAAATTCATTACTGGCAGGAAAATGGCTTAACAGTTGTTAGACTTATGGTTGTCGGCTTCATAGGGAGAATAACATGAGCATTATTAAAGAAtttg               >  1:557231/1‑148 (MQ=255)
                                                                                                                                        ataaGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGTTAGACTTATGGTTGTCGGCTTCATAGGTAGAATAACATGAGCATTATTAAAGAATTTcg              >  2:163164/1‑149 (MQ=255)
                                                                                                                                        ataaGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGTTAGACTTATGGTTGTCGGCTTCATAGGGAGAATAACATGAGCATTATTAAAGAATTTcg              <  1:923846/149‑1 (MQ=255)
                                                                                                                                         taaGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTTGCAGGAAAATGGCTTAACATTTGTTAGACTTATGGTTGTCTGCTTCATAGGGAGAATAACATTAGCATTATTAAAGAATTTcgc             >  2:165818/1‑149 (MQ=255)
                                                                                                                                         taaGGCGTCATTAGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGTTAGACTTATGGTTGTCGGCTTCATAGGGAGAATAACATGAGCATTATTAAAGAATTTcgc             >  1:479505/1‑149 (MQ=255)
                                                                                                                                          acggcggcTACTGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGTTAGACTTATGGTTGTCGGCTTCATAGGGAGAATAACATGAGCATTATTAAAGAATTTcgcg            <  2:946341/147‑1 (MQ=255)
                                                                                                                                           aGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGTTAGACTTATGGTTGTCGGCTTCATAGGGAGAATAACATGAGCATTATTAAAGAATTTCGCGa           >  1:287247/1‑149 (MQ=255)
                                                                                                                                                tCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTGCTGGCAGGAAAATGGCTTAACATTTGTTAGACTTATGGTTGTCGGCTTCATAGGGAGAATAACATGAGCATTATTAAAGAATTTCGCGAATTTg      <  2:96825/149‑1 (MQ=255)
                                                                                                                                                tCTAATGACGAATTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGTCATGAAAATGGCTTAAAATTTGTTAGACTTATTTTTGTCGGCTTCATAGGTAGAATAACATGAGCATTATTAAAGAATTTCGCGAATTTg      <  2:471867/149‑1 (MQ=255)
                                                                                                                                                 cTAATGACGCCTTATTATTTGCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGTTAGACTTATGGTTGTCGGCTTCATAGGGAGAATAACATGAGCATTATTAAAGAATTTCGCGAATTTGc     <  1:428921/149‑1 (MQ=255)
                                                                                                                                                    aTGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGTTAGACTTATGGTTGTCGGCTTCATAGGGAGAATAACATGAGCATTATTAAAGAATTTCGCGAATTTGCGAt  <  2:871357/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
TGGAAACGACGTGATGATTGCCAATGACAATCTGCGCATTGAGCAAGGCGATCACGTAATTATGTTCCTCACAGATAAAAAGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGTTAGACTTATGGTTGTCGGCTTCATAGGGAGAATAACATGAGCATTATTAAAGAATTTCGCGAATTTGCGAT  >  NC_000913/3437762‑3438058

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: