Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,437,911 T→A 7.1% intergenic (+17/‑113) trkA → / → mscL NAD‑binding component of TrK potassium transporter/mechanosensitive channel protein, high conductance

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,437,9110TA 7.1% 277.7 / 5.1 115intergenic (+17/‑113)trkA/mscLNAD‑binding component of TrK potassium transporter/mechanosensitive channel protein, high conductance
Reads supporting (aligned to +/- strand):  ref base T (43/63);  new base A (3/6);  total (46/69)
Fisher's exact test for biased strand distribution p-value = 7.39e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 116 total aligned reads displayed.

GAAACGACGTGATGATTGCCAATGACAATCTGCGCATTGAGCAAGGCGATCACGTAATTATGTTCCTCACAGATAAAAAGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGTTAGACTTATGGTTGTCGGCTTCATAGGGAGAATAACATGAGCATTATTAAAGAATTTCGCGAATTTGCGATG  >  NC_000913/3437764‑3438059
                                                                                                                                                   |                                                                                                                                                    
gAAACGAGGTGATGATTGCCAATGACAATCTGCGCAGTGAGCAAGGAGATCACGTAATTATGTTCCTCACAGATAAAAAGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTGCTTCTTGTAATTAATAAGGCGTCTAATg                                                                                                                                                     >  2:723823/1‑149 (MQ=255)
 aaaCGACGTGATGATTGCCAATGACAATCTGCGCATTGAGCAAGGCGATCACGTAATTATGTTCCTCACAGATAAAAAGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGa                                                                                                                                                    <  1:109919/149‑1 (MQ=255)
          gatgatTGCCAATGACAATCTGCGCATTGAGCAAGGCGATCACGTAATTATGTTCCTCACAGCTAAAAAGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCATTAGACGCCttatt                                                                                                                                           <  2:483012/149‑1 (MQ=255)
          gatgatTGCCAATGACAATCTGCGCATTGAGCAAGGCGATCACGTAATTATGTTCCTCACAGATAAAAAGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCttatt                                                                                                                                           >  1:3169/1‑149 (MQ=255)
          gatgatTGCCAATGACAATCTGCGCATTGAGCAAGGCGATCACGTAATTATGTTCCTCACAGATAAAAAGTTTATAACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCttatt                                                                                                                                           >  1:876263/1‑149 (MQ=255)
           atgatTGCCAATGACAATCTGCGCATTGAGCAAGGCGATCACGTAATTATGTTCCTCACAGATAAAAAGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCttatta                                                                                                                                          <  2:608848/149‑1 (MQ=255)
            tgatTGCCAATGACAATCTGCGCATTGAGCAAGGCGATCACGTAATTATGTTCCTCACAGATAAAAAGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCATTAGACGCCttattaa                                                                                                                                         <  2:882971/149‑2 (MQ=255)
             gatTGCCAATGACAATCTGCGCATTGAGCAAGGCGATCACGTAATTATGTTCCTCACAGATAAAAAGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTattatt                                                                                                                                        >  2:27715/1‑149 (MQ=255)
              atTGCCAATGACAATCTGCGCATTGAGCAAGGCGATCACGTAATTATGTTCCTCACAGATAAAAAGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTAttt                                                                                                                                       >  2:981039/1‑149 (MQ=255)
              atTGCCAATGACAATCTGCGCATTGAGCAAGGCGATCACGTAATTATGTTCCTCACAGATAAAAAGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTAttt                                                                                                                                       >  2:933067/1‑149 (MQ=255)
              atTGCCAATGACAATCTGCGCATTGAGCAAGGCGATCACGTAATTATGTTCCTCACAGATAAAAAGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTAttt                                                                                                                                       >  2:637481/1‑149 (MQ=255)
               ttGCCAATGACAATCTGCGCATTGAGCAAGTCGATCACGTAATTATGTTCCTCACAGATAAAAAGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGAGGCCTTATTATTTc                                                                                                                                      >  1:325966/1‑149 (MQ=255)
               ttGCCAATGACAATCTGCGCATTGAGCAAGGCGATCACGTAATTATGTTCCTCACAGATAAAAAGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCATTAGACGCCTTATTAatta                                                                                                                                      <  1:1007968/149‑2 (MQ=255)
               ttGCCAATGACAATCTGCGCATTGAGCAAGGCGATCACGTAATTATGTTCCTCACAGATAAAAAGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCATTCTTCTTGTAATTAATAAGGCGTCATTAGACGCCTTATTAatta                                                                                                                                      <  1:167076/149‑2 (MQ=255)
               ttGCCAATGACAATCTGCGCATTGAGCAAGGCGATCACGTAATTATGTACCTCACAGATAAAAAGTTTATTACAGACGTCCAAAGACTCTTCCATCAAAGCCATTTATTCTTGTAATTAATAATGCGAATAAATACGCCatataaatta                                                                                                                                      >  2:98537/1‑143 (MQ=255)
                 gCCAATGACAATCTGCGCATTGAGCAAGGCGATCACGTAATTATGTTCCTCACAGATAAAAAGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTccc                                                                                                                                    <  1:459142/149‑1 (MQ=255)
                  ccAATGACAATCTGCGCATTGAGCAAGGCGATCACGTAATTATGTTCCTCACAGATAAAAAGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCt                                                                                                                                   >  1:96825/1‑149 (MQ=255)
                  ccAATGACAATCTGCGCATTGAGCAAGGCGATCACGTAATTATGTTCCTCACAGATAAAAAGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGGCATTAGACGCCTTATTAattacaa                                                                                                                                   <  1:837338/149‑5 (MQ=255)
                  ccAATGACAATCTGCGCATTGAGCAAGGCGATCACGTAATTATGTTCCTAACAGATAAAAAGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAAAGACGCCTTATTATTTCCCt                                                                                                                                   <  2:314138/149‑1 (MQ=255)
                   cAATGACAATCTGCGCATTGAGCAAGGCGATCACGTAATTATGTTCCTCACAGATAAAAAGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCtt                                                                                                                                  <  1:637481/149‑1 (MQ=255)
                    aaTGACAATCTGCGCATTGAGCAAGGCGATCACGTAATTATGTTCCTCACAGATAAAAAGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCttt                                                                                                                                 <  1:82824/149‑1 (MQ=255)
                     aTGACAATCTGCGCATTGAGCAAGGCGATCACGTAATTATGTTCCTCACAGATAAAAAGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTg                                                                                                                                >  2:124574/1‑149 (MQ=255)
                      tGACAATCTGCGCATTGAGCAAGGCGATCACGTAATTATGTTCCTCACAGATAAAAAGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGa                                                                                                                               <  2:156878/149‑1 (MQ=255)
                      tGACAATCTGCGCATTGAGCAAGGCGATCACGTAATTATGTTCCTCACAGATAAAAAGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGa                                                                                                                               <  1:642348/149‑1 (MQ=255)
                       gACAATCTGCGCATTGAGCAAGGCGATCACGTAATAATGTTCCTCACAGATAAAAAGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGAt                                                                                                                              <  1:975508/149‑1 (MQ=255)
                         cAATCTGCGCATTGAGCAAGGCGATCACGTAATTATGTTCCTCACAGATAAAAAGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCGTTCTTCTAGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTa                                                                                                                            >  2:428921/1‑149 (MQ=255)
                            tCTGCGCATTGAGCAAGGCGATCACGTAATTATGTTCCTCACAGATAAAAAGTTTATTAACTACGTCGAAAGACTCTTCCAGCCAAGCCCGTTCTTCTTGTAATTAATAAGGCGTCTAATGACTCCTTATTATTTCCCTTTGATTATCa                                                                                                                         >  2:926680/1‑149 (MQ=255)
                             cTGCGCATTGAGCAAGGCGATCACGTAATTATGTTCCTCACAGATAAAAAGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCaa                                                                                                                        <  2:319441/149‑1 (MQ=255)
                                 gcATTCAGCAAGGCGATCACGTAATTATGTTCCTGACAGATAAAAAGTTTATTACAGACGTAGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGat                                                                                                                    <  1:85448/149‑1 (MQ=255)
                                   aTTGAGCAAGGCGATCACGTAATTATGTTCCTCACAGATAAAAAGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGatta                                                                                                                  >  1:871357/1‑149 (MQ=255)
                                     tGAGCAAGGCGATCACGTAATTATGTTCCTCACAGATATAAAGTTTATTACCGACGTCGAAAGACTCTTCCATCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGattaat                                                                                                                <  1:99558/149‑1 (MQ=255)
                                         cAAGGCGATCACGTAATTATGTTCCTCACAGATAAAAAGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTaaa                                                                                                            <  2:471085/149‑1 (MQ=255)
                                         cAAGGCGATCACGTAATTATGTTCCTCACAGATAAAAAGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTaaa                                                                                                            <  2:234192/149‑1 (MQ=255)
                                         cAAGGCGATCACGTAATTATGTTCCTCACAGATAAAAAGTTTATTACCGACGTCGAAAGACTCTTCCAGCAAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTaaa                                                                                                            <  1:425241/149‑1 (MQ=255)
                                               gctCACGTAATTATGTTCCTCACAGATAAAAAGTTTATTACCGACGTCGAAAGACGCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGACTTATTATTTCCCTTTGATTATCAAGGATTAATTTAattcatt                                                                                                      >  2:352942/3‑149 (MQ=255)
                                                    cGTAATTATGTTCCTCACAGATAAAAAGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTgg                                                                                                 >  2:736711/1‑149 (MQ=255)
                                                      tAATTATGTTCCTCACAGATAAAAAGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCa                                                                                               <  1:499409/149‑1 (MQ=255)
                                                               tCCTCACAGATAAAAAGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATgg                                                                                      <  1:586325/149‑1 (MQ=255)
                                                               tCCTCACAGATAAAAAGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATgg                                                                                      <  2:713218/149‑1 (MQ=255)
                                                                ccTCACAGATAAAAAGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGc                                                                                     <  1:147918/149‑1 (MQ=255)
                                                                  tCACAGATAAAAGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTa                                                                                  >  2:861765/1‑149 (MQ=255)
                                                                    acaGATAAAAAGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTATTGACGCCTTATTATTTCCCTTTGATTATAAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGTCTTaa                                                                                 >  1:307447/1‑149 (MQ=255)
                                                                    acaGATAAAAAGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATAAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTaa                                                                                 >  2:143451/1‑149 (MQ=255)
                                                                     caGATAAAAAGTTTATTACCGACTTCGAAATACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAAc                                                                                <  1:226310/149‑1 (MQ=255)
                                                                      aGATACAAAGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCATGTAATTAATAATGCGACTAATGACGCCTTATTATTTACCTTTGATTATCAACGATTAATAAAATTCATTCCTGGCAGCAAAATGGCTTAACa                                                                               <  2:469105/149‑1 (MQ=255)
                                                                      aGATAAAAAGTTTATTACCGACGTCGAAAGACTCTTCCAGCCACGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATCTCGCCTTATTATTTCCCTTTGTTTATCAAGGATTATTTAAATTCATTCCTGGCAGGAAATTGGCGTAACa                                                                               >  1:682063/1‑149 (MQ=255)
                                                                      aGATAAAAAGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTACCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACa                                                                               <  2:3169/149‑1 (MQ=255)
                                                                       taTAAAAAGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGACCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACAt                                                                              <  2:482067/148‑1 (MQ=255)
                                                                         tAAAAAGTATAATACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCATCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTAACAAGCATTAATTAAATTCAAACATGGCAGGAAATTGGCTTAAAAttt                                                                            >  2:432786/1‑149 (MQ=255)
                                                                          aaaaaGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTATTTAAATTCATTCCTGGCAGGAAAATGGCTTAAAATTTg                                                                           >  1:471867/1‑149 (MQ=255)
                                                                           aaaaGTTTATTACAGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTTATAAGGCGTCTAATGACGCCTTATGTTTTGCCTTTGATTATCTAGGATTAATTAAATTCATTACTGGCAGGAAAATGGCTTAACATTTgg                                                                          >  2:498568/1‑148 (MQ=255)
                                                                            aaaGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGtt                                                                         <  1:121460/149‑1 (MQ=255)
                                                                             aaGTTTATTTCCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTGTTGTAATTAATAATGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGTTa                                                                        <  1:510527/149‑1 (MQ=255)
                                                                               gTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGTTAGa                                                                      <  1:211029/149‑1 (MQ=255)
                                                                               gTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGTTAGa                                                                      <  1:498739/149‑1 (MQ=255)
                                                                                  tattaCCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATACGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATGAAGGATTAATTTAATTAATTCGTGGGAGGTAAATGGCTTTTCATTTTTTAGACtt                                                                   >  2:919986/1‑149 (MQ=255)
                                                                                  tattaCCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGTTAGACtt                                                                   <  1:408306/149‑1 (MQ=255)
                                                                                      aCCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGTTAGACTTATgg                                                               <  1:810409/149‑1 (MQ=255)
                                                                                               gAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTAATTCCTGGCAGTAAAATGGCTTAACATTTGTTAGACTTATGGTTGTCGGCt                                                      >  1:1019041/1‑149 (MQ=255)
                                                                                                aaaGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGTTAGACTTATGGTTGTCGGCtt                                                     >  1:775159/1‑149 (MQ=255)
                                                                                                  tgACTCTTCCAGCCAATCCCTTTCTTCTTGTTATTAATAAGGCGTATAATGACGCCTTATTATTTCCCTTTGTTTATCAAGGATTAATTAAATTCATTGCTGGCAGGAAAATGGCTTAACATTTGTTAGACTTATGGTTGTCGTCTTCa                                                   <  1:98537/148‑1 (MQ=255)
                                                                                                   taCTCTTACAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGTTAGACTTATGGTTGTCGGCTTCAt                                                  <  2:208869/148‑1 (MQ=255)
                                                                                                   gACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGTTAGACTTATGGTTGTCGGCTTCAt                                                  <  1:587100/149‑1 (MQ=255)
                                                                                                    aCTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGTTAGACTTATGGTTGTCGGCTTCATa                                                 >  1:1014849/1‑149 (MQ=255)
                                                                                                     atctTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGTTAGACTTATGGTTGTCGGCTTCATAg                                                <  2:793808/148‑1 (MQ=255)
                                                                                                      tctTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGTTAGACTTATGGTTGTCGGCTTCATAgg                                               >  1:1009966/1‑149 (MQ=255)
                                                                                                      tctTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGTTAGACTTATGGTTGTCGGCTTCATAgg                                               <  2:876263/149‑1 (MQ=255)
                                                                                                      tcatCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTACTGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGTTAGACTTATGGTTGTCGGCTGCATAgg                                               <  1:258611/146‑1 (MQ=255)
                                                                                                       ctTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGTTAGACTTATGGTTGTCGGCTTCATAggg                                              <  1:798739/149‑1 (MQ=255)
                                                                                                       ctTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGTTAGACTTATGGTTGTCGGCTTCATAggg                                              <  1:42872/149‑1 (MQ=255)
                                                                                                        ttCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGTTAGACTTATGGTTGTCGGATTCATAGGga                                             >  1:746934/1‑149 (MQ=255)
                                                                                                         tCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTTATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGTTAGACTTATGGTTGTCGGCTTCATAGGgag                                            >  2:699856/1‑149 (MQ=255)
                                                                                                                 aGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAATGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGTTAGACTTATGGTGGTCGGCTTCATAGGGAGAATAACAt                                    <  2:717334/149‑1 (MQ=255)
                                                                                                                 aGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGTTAGACTTATGGTTGTCGGCTTCATAGGGAGAATAACAt                                    <  1:981039/149‑1 (MQ=255)
                                                                                                                  gCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGTTAGACTTATGGTTGTCGGCTTAATAGGGAGAATAACAtt                                   >  2:59394/1‑148 (MQ=255)
                                                                                                                   cccTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGTTAGACTTATGGTTGTCGGCTTCATAGGGAGAATAACATGa                                  <  1:861765/149‑1 (MQ=255)
                                                                                                                     cTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGTTAGACTTATGGTTGTCGGCTTCATAGGGAGAATAACATGAGc                                <  1:736711/149‑1 (MQ=255)
                                                                                                                     cTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGTTAGACTTATGGTTGTCGGCTTCATAGGGAGAATAACATGAGc                                <  1:739969/149‑1 (MQ=255)
                                                                                                                      tttCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATGTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGTTAGACTTATGGTTGTCGGCTTCATAGGGAGAATAACATGAGCa                               >  1:563308/1‑149 (MQ=255)
                                                                                                                            cttGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGTTAGACTTATGGTTGTCGGCTTCATAGGGAGAATAACATGAGCAttatta                         <  1:980472/149‑1 (MQ=255)
                                                                                                                             ttGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGTTAGACTTATGGTTGTCGGCTTCATAGGGAGAATAACATGAGCATTATTaa                        >  2:357670/1‑149 (MQ=255)
                                                                                                                              tGTAATTAATTAGTCGTCTAATGTCGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGTAAATGGCTTAACCTTTGTTAGACTTATGGTTGGCGGCGTCATAGGGAGAATAACATGAGCATTATTaaa                       <  1:664659/149‑1 (MQ=255)
                                                                                                                              tGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGTTAGACTTATGGTTGTCGGCTTCATAGGGAGAATAACATGAGCATTATTaaa                       <  2:187984/149‑1 (MQ=255)
                                                                                                                               gTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGTTAGACTTATGGTTGTCGGCTTCATAGGGAGAATAACATGAGCATTATTAAAg                      >  1:37508/1‑149 (MQ=255)
                                                                                                                                  attaatAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGTTAGACTTATGGTTGTCGGCTTCATAGGGAGAATAACATGAGCATTATTAAAGAAt                   >  1:238489/1‑149 (MQ=255)
                                                                                                                                   ataataaGGCGTCATTTGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAAATAAATTCATTACTGGCAGGAAAATGGCTTAACATTTGTTAGACTTATGGTTGACGGCTTCATAGGGTGAATAACATGAGCATTATTAAAGAAtt                  >  2:701340/2‑149 (MQ=255)
                                                                                                                                     aataaGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGTTAGACTTTTGGTTGTCGGCTTCATAGGGAGAATAACATGAGCATTATTAAAGAATTTc                >  1:347834/1‑149 (MQ=255)
                                                                                                                                     aataaGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGTTAGACTTATGGTTGTCGGCTTCATAGGGAGAATAACATGAGCATTATTAAAGAATTTc                <  1:973865/149‑1 (MQ=255)
                                                                                                                                     aataaGGCGTCATTAGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAAGTAAATTCATTACTGGCAGGAAAATGGCTTAACAGTTGTTAGACTTATGGTTGTCGGCTTCATAGGGAGAATAACATGAGCATTATTAAAGAAtttg                >  1:557231/1‑148 (MQ=255)
                                                                                                                                      ataaGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGTTAGACTTATGGTTGTCGGCTTCATAGGTAGAATAACATGAGCATTATTAAAGAATTTcg               >  2:163164/1‑149 (MQ=255)
                                                                                                                                      ataaGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGTTAGACTTATGGTTGTCGGCTTCATAGGGAGAATAACATGAGCATTATTAAAGAATTTcg               <  1:919986/149‑1 (MQ=255)
                                                                                                                                      ataaGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGTTAGACTTATGGTTGTCGGCTTCATAGGGAGAATAACATGAGCATTATTAAAGAATTTcg               <  1:923846/149‑1 (MQ=255)
                                                                                                                                       taaGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTTGCAGGAAAATGGCTTAACATTTGTTAGACTTATGGTTGTCTGCTTCATAGGGAGAATAACATTAGCATTATTAAAGAATTTcgc              >  2:165818/1‑149 (MQ=255)
                                                                                                                                       taaGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGTTAGACTTATGGTTGTCGGCTTCATAGGGAGAATAACATGAGCATTATTAAAGAATTTcgc              <  1:124574/149‑1 (MQ=255)
                                                                                                                                        acggcggcTACTGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGTTAGACTTATGGTTGTCGGCTTCATAGGGAGAATAACATGAGCATTATTAAAGAATTTcgcg             <  2:946341/147‑1 (MQ=255)
                                                                                                                                         aGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGTTAGACTTATGGTTGTCGGCTTCATAGGGAGAATAACATGAGCATTATTAAAGAATTTCGCGa            >  1:287247/1‑149 (MQ=255)
                                                                                                                                              tCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTGCTGGCAGGAAAATGGCTTAACATTTGTTAGACTTATGGTTGTCGGCTTCATAGGGAGAATAACATGAGCATTATTAAAGAATTTCGCGAATTTg       <  2:96825/149‑1 (MQ=255)
                                                                                                                                               cTAATGACGCCTTATTATTTGCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGTTAGACTTATGGTTGTCGGCTTCATAGGGAGAATAACATGAGCATTATTAAAGAATTTCGCGAATTTGc      <  1:428921/149‑1 (MQ=255)
                                                                                                                                                  aTGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGTTAGACTTATGGTTGTCGGCTTCATAGGGAGAATAACATGAGCATTATTAAAGAATTTCGCGAATTTGCGAt   <  2:871357/149‑1 (MQ=255)
                                                                                                                                                   tGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGTTAGACTTATGGTTGTCGGCTTCATAGGGAGAATAACATGAGCATTATTAAAGAATTTCGCGAATTTGCGATg  >  2:642135/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
GAAACGACGTGATGATTGCCAATGACAATCTGCGCATTGAGCAAGGCGATCACGTAATTATGTTCCTCACAGATAAAAAGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGTTAGACTTATGGTTGTCGGCTTCATAGGGAGAATAACATGAGCATTATTAAAGAATTTCGCGAATTTGCGATG  >  NC_000913/3437764‑3438059

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: