Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,635,236 G→A 6.1% L132L (CTG→CTA)  yhiM → acid resistance protein, inner membrane

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,635,2360GA 6.1% 159.2 / 2.2 65L132L (CTG→CTA) yhiMacid resistance protein, inner membrane
Reads supporting (aligned to +/- strand):  ref base G (32/29);  new base A (2/2);  total (34/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.72e-01

GTCAATTATCACCATGATATGGGGTTGGGCACTGTTAGCAGGCAATGATGTGATGGCAGACGAGTTTGTCGCCGGCCATGTTATTTTCGGCGTTGGTATGATTGCCGCCTGTGTATCGACGGTGGCAGCGTCATCCGGTCACTTTCTGCTCATTCCCAAAAATGCAGCGGGGAGCAAGAGCGACGGAACACCGGTACAGGCTTATTCTTCATTAATCGGTAACTGCCTCATTGCCGTTCCCGTTTTACTCACCCTGCTCGGTTTCATTTGGTCTATTACGCTGTTACGTAGTG  >  NC_000913/3635089‑3635381
                                                                                                                                                   |                                                                                                                                                 
gTCAATTATCACCATGATATGGGGTTGGGCACTGTTAGCAGGCAATGATGTGATGGCAGACGAGTTTGTCGCCGGCCATGTTATTTTCGGCGTTGGTATGATTGCCGCCCGTGTATCGACGGTGGCAGCGTCATCCGGTCACTTTCTGc                                                                                                                                                  <  1:362143/149‑1 (MQ=255)
   aaTTATCACCATGATATGGGGTTGGGCACTGTTAGCAGGCAATGATGTGATGGCAGACGAGTTTGTCGCCGGCCATGTTATTTTCGGCGTTGGTATGATTGCCGCCTGTGTATCGACGGTGGCAGCGTCATCCGGTCACCTTCTGCTCa                                                                                                                                               <  1:893963/149‑1 (MQ=255)
    aTTATCACCATGATATGGGGTTGGGCACTGTTAGCAGGCAATGATGTGATGGCAGACGAGTTTGTCGCCGGCCATGTTATTTTCGGCGTTGGTATGATTGCCGCCTGTGTATCGACGGTGGCAGCGTCATCCGGTCACTTTCTGCTCAt                                                                                                                                              >  2:84973/1‑149 (MQ=255)
    aTTATCACCATGATATGGGGTTGGGCACTGTTAGCAGGCAATGATGTGATGGCAGACGAGTTTGTCGCCGGCCATGTTATTTTCGGCGTTGGTATGATTGCCGCCTGTGTATCGACGGTGGCAGCGTCATCCGGTCACTTTCTGCTCAt                                                                                                                                              <  2:27751/149‑1 (MQ=255)
      tATCACCATGATATGGGGTTTGGCACTGTTAGCAGGCAATGATGTGATGGGAGACGAGTTTGTCGCCGGCCATGTTATTTTCGGCGTTTGTATGATTGCCGCCTGTGTATCGACGGTGGCAGCGTCATCCGGTCACTTTCTGCCCATTc                                                                                                                                            <  1:330620/149‑1 (MQ=255)
         cACCATGATATGGGGTTGGGCACTGTTAGCAGGCAATGATGTGATGGCAGACGAGTTTGTCGCCGGCCATGTTATTTTCGGCGTTGGTATGATTGCCGCCTGTGTATCGACGGTGGCAGCGTCATCCGGTCACTTTCTGCTCATTCCCa                                                                                                                                         >  1:953530/1‑149 (MQ=255)
            cATGATATGGGGTTGGGCACTGTTAGCAGGCAATGATGTGATGGCAGACGAGTTTGTCGCCGGCCATGTTATTTTCGGCGTTGGTATGATTGCCGCCTGTGTATCGACGGTGGCAGCGTCATCCGGTCACTTTCTGCTCATTCCCaaaa                                                                                                                                      >  1:796891/1‑149 (MQ=255)
             aagATATGGGGTTGGCCACTGTTAGCAGGCAATGATGGGATGGCAGACGAGGTTGTCGCCGGCCATGTTATTTTCGGCGTTGGCATGATTGCCGCCTGTGTATCGACGGTGGCAGCGTCATCCGGTCACTTTCTGCTCATTCCCaaaaa                                                                                                                                     <  1:54124/147‑1 (MQ=255)
               gATATGGGGTTGGGCACTGTTAGCAGGCAATGATGTGATGGCAGACGAGTTTGTCGCCGGCCATGATATTTTCGGCGTTGGTATGATTGCCGCCTGTGTATCGACGGTGGCAGCGTCATCCGGTCACTTTCTGCTCATTCCCAAAAATg                                                                                                                                   >  2:732781/1‑149 (MQ=255)
                 tatGGGGGTTGGCACTGTTTGCACGCATTGATGTGATGGCAGACGAGTTTGTCGCCGGCCATGTTATTTTCGGCGTTCGTATGATTGCCGCCTGTGTATCGACGGTGGCAGCGTCATCCAGTCACTTTCTGCTCAATCCCAAAAATGCa                                                                                                                                 <  1:964163/149‑1 (MQ=255)
                   tGGGGTTGGGCACTGTTAGCATGCAATGATGTGATGGGAGACGATTTTGTCGCCGGCAAAGTTATTTTCGGCGTTGGTATGATTGCCGCCTGTGTATCGACGGTGGCAGCGTCATCCGGTCACTTTCTGCTCATTCCCAAAAATGCAGc                                                                                                                               <  1:902992/149‑1 (MQ=255)
                   tGGGGTTGGGCACTGTTAGCAGGCATTGATGTGATGGCAGCCGAGGTTGTCGCCGGCCAGGTTATTTTCGGCGGTGGGATGATTGCGGCGTGTGTATCGACGGTGGCAGCGTCATCCGGTCACTTTGTGCTCATTCCCAACAATGCCGc                                                                                                                               >  2:81016/1‑149 (MQ=255)
                      ggTTGGGCACTGTTAGCAGGCAATGATGTGATGGCAGACGAGGTTGTCGCCGGCCATGTTATTTTCGGCGTTGGTATGATTGCCGCCTGTGTATCGACGGTGGCAGCGTCATCCGGTCACTTTCTGCTCATTCCCAAAAATGCAGCggg                                                                                                                            <  1:393386/149‑1 (MQ=255)
                            gCACTGTTAGCAGGCAATGATGTGATGGCAGACGAGTTTGTCGCCGGCCATGTTATTTTCGGCGTTGGTATGATTGCCGCCTGTGTATCGACGGTGGCAGCGTCATCCGGTCACTTTCTGCTCATTCCCAAAAATGCAGCGGGGAGCaa                                                                                                                      >  1:757232/1‑149 (MQ=255)
                             cACTGTTAGCAGGCAATGATGTGATGGCAGACGAGTTTGTCGCCGGCCATGTTATTTTCGGCGTTGGTATGATTGCCGCCTGTGTATCGACGGTGGCAGCGTCATCCGGTCACTTTCTACTCATTCCCAAAAATGCAGCGGGGAGCAag                                                                                                                     >  1:369403/1‑149 (MQ=255)
                             cACTGTTAGCAGGCAATGATGTGATGGCAGACGAGTTTGTCGACGGCCATGTTATTTTCGGCGTTGGTATGATTGACGCCTGTGTATCGACGGTGGCAGCGTCATCCGGTAACTTTCTACTCATTCCCAAAAATGAAGCGGGGAGCAag                                                                                                                     >  1:978988/1‑149 (MQ=255)
                                       tggCATTGATGTGATGGCAGAGGAGTTTGTCGCCGGCATTGTTATTTTCGGCGTTGGTATGAATGCCGCCTGTGTATCGACGGTGGCAGCGTCATCCGGTCACTTTCTGCTCATTCCCAAAAATGCAGAGGGGAGCAAGAGCGACGGaa                                                                                                           <  2:142863/148‑1 (MQ=255)
                                       aGGCAATGATGTGATGGCAGACGAGTTTGTCGCCGGCCATGTTATTTTCGGCGTTGGTATGATTGCCGCCTGTGAATCGACGGTGGCAGCTTCATCCGGTCACTTTCTGCTCATTCCCTAAAATGCAGCGGGGAGCAAGAGCGACTGaa                                                                                                           >  2:260906/1‑149 (MQ=255)
                                          cAATGATGTGATGGCAGACGAGTTTGTCGCCGGCCATGTTATTTTCGGCGTTGGTATGATTGCCGCCTGTGTATCGACGGTTGCAGCGTCATCCGGTCACTTTCTGCTCATTCCCAAAAATGCAGCGGGGAGCAAGAGCGACGGAacac                                                                                                        >  1:851839/1‑149 (MQ=255)
                                            atgatgTGATGGCAGACGAGTTTGGCGCCGGCCATGTTATTTTCGGCGTTGGTATGATTGCCGCCTGTGTATCGACGGTGGCAGCGTCATCCGGTCACTTTCTGCTCATTCCCAAAAATGCAGCGGGGAGCAAGAGCGACGGAACACCg                                                                                                      <  1:430386/149‑1 (MQ=255)
                                             tgatgtgatgGCAGACGAGTTTGTCGCCGGCCATGTTATTTTCGGCGTTGGTATGATTGCCGCCTGTGTATCGACGGTGGCAGCGTCATCCGGTCACTTTCTGCTCATTCCCAAAAATGCAGCGGGGAGCAAGAGCGACGGAACACCgg                                                                                                     >  1:223550/1‑149 (MQ=255)
                                             tgatgtgatgGCAGACGAGTTTGTCGCCGGCCATGTTATTTTCGGCGTTGGTATGATTGCCGCCTGTGTATCGACGGTGGCAGCGTCATCCGGTCACTTTCTACTCATTCCCAAAAATGCAGCGGGGAGCAAGAGCGACGGAACACCgg                                                                                                     <  2:369403/149‑1 (MQ=255)
                                             tgatgtgatgGCAGACGAGTTTGTCGCAGGCCATGTTAATTTCGGCGTTGGTATGATTGCCGCCTGGGTATCGACGGTGGCAGCGTCATCCGGTCACTTTCTACTCATTCCCAAAAATGCAGCGGGGAGCAAGAGCGACGGAACACCgg                                                                                                     <  2:978988/149‑1 (MQ=255)
                                                 gtgatgGCAGACGAGTTTGTCGCCGGCCATGTTATTTTCGGCGTTGGTATGATTGCCGCCTGTGTATCGACGGTGGCAGCGTCATCCGGTCACTTTCTGCTCATTCCCAAAAATGCAGCGGTGAGCAAGAGCGACGGAACACCGGTACa                                                                                                 >  1:721134/1‑149 (MQ=255)
                                                        cAGACGAGTTTGTCGCCGGCCATGTTATTTTCGGCGTTGGTATGATTGCCGCCTGTGTATCGACGGTGGCAGCGTCATCCGGTCACTTTCTGCTCATTCCCAAAAATGCAGCGGGGAGCAAGAGCGACGGAACACCGGTACAGGCTTAt                                                                                          >  2:1005529/1‑149 (MQ=255)
                                                        cAGACGAGTTTGTCGCCGGCCATGTTATTTTCGGCGTTGGTATGATTGCCGCCTGTGTATCGACGGTGGCAGCGTCATACGGTCACTTTCTGCTCATTCCCAAAAATGCAGCGGGGAGCAAGAGCGACGGACCACCGGTACAGGCTTAt                                                                                          >  2:1001616/1‑149 (MQ=255)
                                                             gAGTTTGTCGCCGGCCATGTTATTTTCGGCGTTGGTATGATTGCCGCCTGTGTATCGACGGTGGCAGCGTCATCCGGTCACTTTCTGCTCATTCCCAAAAATGCAGCGGGGAGCAAGAGCGACGGAACACCGGTACAGGCTTAttcttc                                                                                     >  1:416768/1‑149 (MQ=255)
                                                                tttGTCGCCGGCCATGTTATTTTCGGCGTTGGTATGATTGCCGCCTGTGTATCGACGGTGGCAGCGTCATCCGGTCACTTTCTGCTCATTCCCAAAAATGCAGCGGGGAGCAAGAGCGACGGAACACCGGTACAGGCTTATTCTTCAtt                                                                                  >  1:336516/1‑149 (MQ=255)
                                                                  tGTCGCCGGCCATGTTATTTTCGGCGTTGGTATGATTGCCGCCTGTGTATCGACGGTGGCAGCGTCATCCGGTCACTTTCTGCTCATTCCCAAAAATGCAGTGGGGAGCAAGAGCGACGGAACACCGGTACAGGCTTATTCTTCATTaa                                                                                <  1:478009/149‑1 (MQ=255)
                                                                   ggcGCCGGCCATGGTATTTTCGGCGTTGGTATGATTGCCGCCTGTGTATCGTCGGTGGCAGCGACATCCGGTCACTTTCTGCTCAATCCCAAAAATGCAGCGGGGAGCAAGAGCGACGGAACACCGGTACAGGCTTATTCTTCATTAAt                                                                               <  2:633122/147‑1 (MQ=255)
                                                                    tCGCCGGCCATGTTATTTTCGGCGTTGGTATGATTGCCGCCTGTGTATCGACGGTGGCAGCGTCATCCGGTCACTTTCTGCTCATTCCCAAAAATGCAGCGGGGAGCAAGAGCGACGGAACACCGGTACAGGCTTATTCTTCATTAATc                                                                              >  1:269677/1‑149 (MQ=255)
                                                                     ggCCTGCCATGTTATTTTCGGCGTTGGTTTGATTGCCGCCTGTGTATCGACGGTGGCAGCGTCATCCGGTCACTTTCTGCTCATTCCCAAAAATGCAGCGGGGAGCAAGAGCGACGGAACACCGGTACAGGCTTATTCTTCATTAATCg                                                                             <  1:86994/148‑1 (MQ=255)
                                                                       ccGGCCATGTTATTTTCGGCGTTGGTATGATTGCCGCCTGTGTATCGACGGTGGCAGCGTCATCCGGTCACTTTCTGCTCATTCCCAAAAATGCAGCGGGGAGCAAGAGCGACGGAACACCGGTACAGGCTTATTCTTCATTAATCGGt                                                                           >  1:901206/1‑149 (MQ=255)
                                                                        cGGCCATGTTATTTTCGGCGTTGGTATGATTGCCGCCTGTGTATCGACGGTGGCAGCGTCATCCGGTCACTTTCTGCTCATTCCCAAAAATGCAGCGGGGAGCAAGAGCGACGGAACACCGGTACAGGCTTATTCTTCATTAATCGGTa                                                                          >  2:528340/1‑149 (MQ=255)
                                                                            cATGTTATTTTCGGCGTTGGTATGATTGCCGCCTGTGTATCGACGGTGGCAGCGTCATCCGGTCACTTTCTGCTCATTCCCAAAAATGCAGCGGGGAGCAAGAGAGACGGAACACCGGTACAGGCTTATTCTTCATTAATCGGTAACTg                                                                      >  2:685770/1‑149 (MQ=255)
                                                                              tGTTATTTTCGGCGTTGGTATGATTGCCGCCTGTGTATCGACGGTGGCAGCGTCATCCGGTCACTTTCTGCTCATTCCCAAAAATGCAGCGGGGAGCAAGAGCGACGGAACACCGGTACAGGCTTATTCTTCATTAATCGGTAACTGcc                                                                    <  1:84973/149‑1 (MQ=255)
                                                                                ttATTTTCGGCGTTGGTATGATTGCCGCCTGTGTATCGACGGTGGCAGCGTCATCCGGTCACTTTCTGCTCATTCCCAAAAATGCAGCGGGGAGCAAGAGCGACGGAACACCGGTACAGGCTTATTCTTCATTAATCGGTAACTGCCTc                                                                  <  2:416768/149‑1 (MQ=255)
                                                                                        ggCGTTGGTATGATTGCCGCCTGTGTATCGACGGTGGCAGCGTCATCCGGTCACTTTCTGCTCATTCCCAAAAATGCAGCGGGGAGCAAGAGCGACGGAACACCGGTACAGGCTTATTCTTCATTAATCGGTAACTGCCTCATTGCCGt                                                          >  2:955347/1‑149 (MQ=255)
                                                                                         gCGTTGGTATGATTGCCGCCTGTGTATCGACGGTGGCAGCGTCATCCGGTCACTTTCTGCTCATTCCCAAAAATGCAGCGGGGAGCAAGAGCGACGGAACACCGGTACAGGCTTATTCTTCATTAATCGGTAACTGCCTCATTGCCGtt                                                         >  1:852256/1‑149 (MQ=255)
                                                                                                 aTGATTGCCGCCTGTGTATCGACGGTGGCAGCGTCATCCGGTCACTTTCTGCTCATTCCCAAAAATGCAGCGGGGAGCAAGAGCGACGGTACACCGGTACAGGCTTATTCTTCATTAATCGGTAACTGCCTCATTGCCGTTCCCGtttt                                                 >  2:779831/1‑149 (MQ=255)
                                                                                                    aTTGCCGCCTGTTTATCGACGGTGGCAGCGTCATCCGGTCACTTTCTGCTCATTCCCAAAAATGCAGCGGGGAGCAAGAGCGACGGAACACCGGTACAGGCTTATTCATCATTAATCGGTAACTGCCTCAATGCCGTTCCCGTTTTACt                                              <  1:820948/149‑1 (MQ=255)
                                                                                                      tGCCGCCTGTGTATCGACGGTGGCAGCGTCATCCGGTCACTTTCTGCTCATTCCCAAAAATGCAGCGGGGAGCAAGAGCGACGGAACACCGGTACAGGCTTATTCTTCATTAATCGGTAACTGCCTCATTGCCGTTCACGTTTTACTCa                                            >  2:31671/1‑149 (MQ=255)
                                                                                                      tGCCGCCTGTGTATCGACGGTGGCAGCGTAATCCGGTCACTTTCTGCTCATTCCCAAAAATGCAGCGGGGAGCAAGAGCGACGGAACACCGGTACAGGCTTATTCTTCATTAATCGGTAACTGCCTCATTGCCGTTCCCGTTTTACTCa                                            <  2:796891/149‑1 (MQ=255)
                                                                                                         cgccTGGGTATCGACGGTGGCAGCGTCATCCGGTCACTTTCTGCTCATTCCCAAAAATGCAGCGGGGAGCAAGAGCGACGGAACACCGGTACAGGCTTATTCTTCATTAATCGGTAACTGCCTCATTGCCGTTCCCGTTTTACTCAccc                                         <  2:223550/149‑1 (MQ=255)
                                                                                                            cTGTGTATCGACGGTGGCAGCGTCATCCGGTCACTTTCTGCTCATTCCCAAAAATGCAGCGGGGAGCTAGAGCGACGGCACACCGGTACAGGCTTATTCTTCATTAATCGGTAACTGCCTCATTGCCGTTCCCGTTTTTCTCACCCTGc                                      >  1:253251/1‑149 (MQ=255)
                                                                                                            cTGTGTATCGACGGTGGCAGCGTCATCCGGTCACTTTCTGCTCATTCCCAAAAATGCAGCGGGGAGCAAGAGCGCCGGAACACCGGTACAGGCTTATTCTTCATTAATCGGTAACTGCCTCATTGCCGTTCCCGTTTTACTCACCCTGc                                      >  1:314340/1‑149 (MQ=255)
                                                                                                             tgtgTATCGACGGTGGCAGCGTCATCCGGTCACTTTCTGCTCATTCCCAAAAATGCAGCGGGGAGCAAGAGCGACGGAACACCGGTACAGGCTTATTCTTCATTAATCGGTAACTGCCTCATTGCCGTTCCCGTTTTACTCACCCTGCt                                     <  1:973887/149‑1 (MQ=255)
                                                                                                              gtgtATCTACGGTGGCAGCGTGATCGGGTCTGTTTCTGGTCATTCCCTAAAATGCAGCGGGGAGCAAGAGCGACGGTACACCGGTACATGCTTATTCTTCATTAATCGGTAACTGCCTCATTGCCGTTCCCGTTTTACTCACCCTGCTc                                    <  2:71010/149‑1 (MQ=255)
                                                                                                              gtgtATCGACGGTGGCAGCGTCATCCGGTCACTTTCTGCTCATTCCCAAAAATGCAGCGGGGAGCAAGAGCGACGGAACACCGGTACAGGCTTATTCTTCATTAATCGGTAACTGCCTCATTGCCGTTCCCGTTTTACTCACCCTGCTc                                    <  2:314990/149‑1 (MQ=255)
                                                                                                                 tATCGACGGTGGCAGCGTCATCCGGTCACTTTCTGCTCATTCCCAAAAATGCAGCGGGGAGCAAGAGCGACGGAACACCGGTACAGGCTTATTCTTCATTAATCGGTAACTGCCTCATTGCCGTTCCCGTTTTACTCACCCTGCTCGGt                                 <  1:1005529/149‑1 (MQ=255)
                                                                                                                 tATCGACGGTGGCAGCGTCATCCGGTCACTTTCTGCTCATTCCCAAAAATGCAGCGGGGAGCAAGAGCGACGGAACACCGGTACAGGCTTATTCTTCATTAATCGGTAACTGCCTCATTGCCGTTCCCGGTTTACTCACCCTGCTCGGt                                 <  1:1001616/149‑1 (MQ=255)
                                                                                                                  aTCGACGGTGGCAGCGTCATCCGGTCACTTTCTGCTCATTCCCAAAAATGCAGCGGGGAGCAAGAGCGACGGAACACCGGTACAGGCTTATTATTCATTAATCGGTAACTGCCTCATTGCCGTTCCCGTTTTACTCACCCTGCTAGGtt                                >  1:572324/1‑149 (MQ=255)
                                                                                                                   tCGACGGTGGCAGCGTCATCCGGTCACTTTCTGCTCATTCCCAAAAATGCAGCGGGGAGCAAGAGCGACGGAACACCGGTACAGGCGTATTCTTCATTAATCGGTAACTGCCTCATTGCCGTTCCCGTTTTACTCACCCTGCTCGGttt                               >  1:333311/1‑149 (MQ=255)
                                                                                                                    cGACGGTGGCAGCGTCATCCGGTCACTTTCTGCTCATTCCCAAAAATGCAGCGGGGAGCAAGAGCGACGGACCACCGGTACAGGCTTATTCTTCATTAATCGGTAACTGCCTCATTGCCGTTCCCGTTTTACTCACCCTGCTCGGTTTc                              <  2:336516/149‑1 (MQ=255)
                                                                                                                      aCGGTGGCAGCGTCATCCGGTCACTTTCTGCTCATTCCCAAAAATGCAGCGGGGAGCAAGAGCGACGGAACACCGGTACAGGCTTATTCTTCATTAATCGGTAACTGCCTCATTGCCGTTCCCGTTTTACTCACCCTGCTCGGTTTCAt                            <  1:844871/149‑1 (MQ=255)
                                                                                                                         gTGGCAGCGTCATCCGGTCACTTTCTGCTCATTCCCAAAAATGCAGCGGGGAGCAAGAGAGACGGAACACCGGTACAGGCTTATTCTTCATTAATCGGTAACTGCCTCATTGCCGTTCCCGTTTTACTCACCCTGCTCGGTTTCATTTg                         <  1:685770/149‑1 (MQ=255)
                                                                                                                          tGGCAGCGTCATCCGGTCACTGTCTGCTCATTCCCAAAAATGCAGCGGGTAGCAAGAGCGACGGAACACCGGTACAGGCTTATTCTTCATTAATCGGAAACTGCCTCATTGCCGTTCCCGATTTACTCACCCTGCTCGGTTTCATTTgg                        <  1:732781/149‑1 (MQ=255)
                                                                                                                            gCAGCGTCATCCGGTCACTTTCTGCTCATTCCCAAAAATGCAGCGGGGCGCAAGAGCGACGGAACACCGGTACAGGCTTATTCTTCATTAATCGGTAACTGCCTCATTGCCGTTCCCGTTTTACTCACCCTGCTCGGTTTCATTTGGTc                      >  1:657701/1‑149 (MQ=255)
                                                                                                                            gCAGCGTCATCCGGTCACTTTCTGCTCATTCCCAAAAATGCAGCGGGGAGCAAGAGCGACGGAACACCGGTACAGGCTTATTCTTCATTAATCGGTAACTGCCTCATTGCCGTTCCCGTTTTACTCACCCTGCTCGGTTTCATTTgggg                      >  2:976612/1‑147 (MQ=255)
                                                                                                                              aGCGTCATCCGGTCACTTTCTGCTCATTCCCAAAAATGCAGCGGGGAGCAAGAGCGACGGAACACCGGTACAGGCTTATTCTTCATTAATCGGTAACTGCCTCATTGCCGTTCCCGTTTTACTCACCCTGCTCGGTTTCATTTGGTCTa                    >  1:539651/1‑149 (MQ=255)
                                                                                                                                 gTCATCCGGTCACTTTCTGCTCATTCCCAAAAATGCAGCGGGGAGCAAGAGCGACGGAACACCGGTACAGGCTTATTCTTCATTAATCGGTAACTGCCTCATTGCCGTTCCCGTTTTACTCACCCTGCTCGGTTTCATTTGGTCTATTa                 >  1:71096/1‑149 (MQ=255)
                                                                                                                                  tCATCCGGTCACTTTCTGCTCATTCCCAAAAATGAAGCGGAGAGCAAGCGCGACGTAACACCGGTACAGGCGTATTCTTCATTACTCGCTCACTTCCTCATTGCCGTTACCGAGTTAGTCACCCTGCTCGGTCTCATTTGGTCAAATAc                >  1:662015/1‑149 (MQ=255)
                                                                                                                                    atacGGTCACTTTCTGCTCATTCCCAAAAATGCAGCGGGGAGCTAGAGCGCCGGTAAACCGGTACAGGCTTATTCGTCATTAATCGGTAACTGCCTCAGTGCCGTGCCCGGTTTACTCACCCTGCTCGGGTTACTGTGGTCTATTACGc              >  2:676531/4‑149 (MQ=255)
                                                                                                                                              tttCTGCCCATTCCCAAAAATGCAGCGGGGAGCAAGAGCGACGGCACACCGGTACAGGCTTATTCTTCATTAATCGGAAACTGCCTCATTGCCGTTCCCTTTTTACTCACCCTGCTCGGTTTCTTTTGGTCTATTACGCTGTTACGTAg    <  1:663181/149‑1 (MQ=255)
                                                                                                                                                tCTGCTCATTCCCAAAAATGCAGCGGGTAGCATGATCGACGGAACACCTGTACAGGCTTATTCTTCATAAATCGGTAACTGACGCATTGCCGTTCCCGTTTTACTCACCCTGCTCGGTTTCATATGGTCTATTACGCTGTTACGTAGTg  <  2:930601/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                 
GTCAATTATCACCATGATATGGGGTTGGGCACTGTTAGCAGGCAATGATGTGATGGCAGACGAGTTTGTCGCCGGCCATGTTATTTTCGGCGTTGGTATGATTGCCGCCTGTGTATCGACGGTGGCAGCGTCATCCGGTCACTTTCTGCTCATTCCCAAAAATGCAGCGGGGAGCAAGAGCGACGGAACACCGGTACAGGCTTATTCTTCATTAATCGGTAACTGCCTCATTGCCGTTCCCGTTTTACTCACCCTGCTCGGTTTCATTTGGTCTATTACGCTGTTACGTAGTG  >  NC_000913/3635089‑3635381

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑