Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,708,595 G→A 5.6% intergenic (‑890/+21) dppA ← / ← proK dipeptide/heme ABC transporter periplasmic binding protein; dipeptide chemotaxis receptor/tRNA‑Pro

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,708,5950GA 5.6% 450.5 / 12.3 178intergenic (‑890/+21)dppA/proKdipeptide/heme ABC transporter periplasmic binding protein; dipeptide chemotaxis receptor/tRNA‑Pro
Reads supporting (aligned to +/- strand):  ref base G (94/72);  new base A (5/5);  total (100/78)
Fisher's exact test for biased strand distribution p-value = 7.50e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.89e-01

Only 100 of 180 total aligned reads displayed.

TTGGTCGGTGATAGAGGATTACTCGCCACTTCGTGGCTCGCCCTGCGGGCCGTTGCTGGCGCAACGTTCTCTCGCTTCGCTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTCGAACCTCCGACCCCTTCGTCCCGAACGAAGTGCGCTACCAGGCTGCGCCAATCACCGAATGCGGGGCGCATCTTACTGCGCAGATACGCCCTCGTCAATCCCTTAATA  >  NC_000913/3708447‑3708743
                                                                                                                                                    |                                                                                                                                                    
ttGGGCGGTGATAGACGATTACTCGCCACTTCGTGGCTCGCCCGGCGGGCCGTTGCTTGCGCAACGTTCTCTCGCTTCGCTCGCGTCTAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCCGATGCAAAAAAGCCTGCTCg                                                                                                                                                      <  2:116199/149‑1 (MQ=25)
    tCGGTGATAGCGGAGTACTCGCCACTTCGTGGCTCGCCCTGCGGGCCGGTGCTGGCGGATCGTTCTCTCGCTTCGCTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGa                                                                                                                                                  <  1:63386/149‑1 (MQ=25)
    tCGGTGATAGAGGATTACTCGCCACTTGGGGGCACGCCCTGCGGGGCGTTGCGGGCTGAACGTTATCTCGCCTCGGTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGa                                                                                                                                                  <  1:955762/149‑1 (MQ=18)
        tGATAGAGGATTACTCGCCACGTCGTGGCTCGCCCTGCGGGCCGTTGCTGGCGCAACGTTCTCTCGCTTCGCTCGAGTCGAACCTCCCTGCCCCCGGCGGGTCTCAGACGCATGCGCTGCAGATGCAAAAAAGCCTGCGCGGTGAGccg                                                                                                                                              >  1:373607/1‑147 (MQ=37)
         taTAGAGGATTAAACGCCACTTGGTGGCTCTCATTGCGGTACGTAGCTGGCGCAACGTTCTCTCGCTTAGCTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAgg                                                                                                                                             <  2:641013/148‑1 (MQ=18)
          atatAGGATTACTCGCCAATTCGTGGCTCGACATGCGGGCCGTTGCTTGCGCAACGTTATCTCGCTTCGCTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCAACGAGCAGGc                                                                                                                                            <  2:16150/149‑1 (MQ=21)
          aTAGAGGATTACTCGCCACTTCGTGGCTCGCCCTGCGGGCCGTTGCTGGCGCAACGTTCTCTCGCTTCGCTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCAGGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGc                                                                                                                                            >  1:975811/1‑149 (MQ=255)
              agGATTACTCGCCACTTCGTGGCTCGCCCTGCGGGCCGTTGCTGGCGCAACGTTCTCTCGCTTCGCTCGAGTCGCGCCTCCCTGCCCCCGGCGGTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCGCGGTGAGCCGGCtttt                                                                                                                                        >  1:175807/1‑149 (MQ=25)
                gATTACTCGCCACTTCGTGGCTCGCCCTGCGGGCCGTTGCTGGCGCAACGTTCTCTCGCTTCGCTCGAGTCGCACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCg                                                                                                                                      >  1:723260/1‑149 (MQ=255)
                ccctACTCGCCACTTCGTGGCTCGCCCAGCGGGCCGTCGCTGGCGCAACGTTCTCTCGCTTCGCTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCAACGAGCAGGCtttttt                                                                                                                                      <  1:425438/146‑3 (MQ=21)
                  ttACTCGCCACTTCGTGGCTCGCCCTGCGGGCCGTTGCTGGCGCAACGTTCTCTCGCTTCGCTCGAGTCGAACCTCCCTGCCCCCGGCGGTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGaa                                                                                                                                    >  2:559824/1‑149 (MQ=255)
                     cTCGCCACTTCGTGGCTCGCCCTGCGGGCCGTTGCTGGCGCAACGTTCTCTCGCTTCGCTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAAttt                                                                                                                                 <  1:62989/149‑1 (MQ=255)
                     cTCGCCACTTCGTGGCTCGCCCTGCGGGCCGTTGCTGGCGCAACGTTCTCTCGCTTCGCTCGAGTCGAACCTCCCGGCCCCCGGAGGTTCGCAGCCTCATGAGCTGCAGCTGGAAAAAAGCCTGCGCGTTGGGCAGGGGGTTCGAAttt                                                                                                                                 >  1:618090/1‑149 (MQ=255)
                      tCGCCACTTCGTGGCTCGCCCTGCGGGCCGTTGCTGGCGCAACGTTCTCTCGCTTCGCTCGAGTCGCACCTCCCTGCCCCCGGAGGTTCTCAGCCGCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCGTTTCGAATTTg                                                                                                                                >  2:74394/1‑149 (MQ=255)
                      tCGCCACTTCGTGGCTCGCCCTGCGGGCCGTTGCTGGCGCAACGTTCTCTCGCTTCGCTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGATGCAAAAAGGCCTGCTCGGGGAGCAGGCGTTTCGAATTTg                                                                                                                                >  1:509297/1‑149 (MQ=255)
                      tCGCCACTTCGTGGCTCGCCCTGCGGGCCGTTGCTGGCGCAACGTTCTCTCGCTTCGCTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCAGCCTCATGAGCTGCCGCTGCAAAAAAGCCTGCTCGTGGCGCAGGCTTTTCGAATTTg                                                                                                                                >  1:331066/1‑149 (MQ=25)
                        gCCACTTCGTGGCTCGCCCTGCGGGCCGTTGCTGGCGCAACGTTCTCTCGCTTCGCTCGAGTCGAACCTCCCTGCCCCCGGCGGTTCTCATCCTCATGAGCTGGAGATGCAAAAAAGCCTGCTCGTAGAGCAGGCTTTTCGAATTTGGt                                                                                                                              >  1:627273/1‑149 (MQ=255)
                        gCCACTTCGTGGCTCGCCCTGCGGGCCGTTGCTGGCGCAACGTTCTCTCGCTTCGCTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGt                                                                                                                              >  2:597529/1‑149 (MQ=255)
                        gCCACTTCGTGGCTCGCCCTGCGGGCCGTTGCTGGCGCAACGTTCTCTCGCTTCGCTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGGTGAGCAGGCTTTTCGAATTTGGt                                                                                                                              >  2:417261/1‑149 (MQ=255)
                           aCTTCGTGGCTCGCCCTGCGGGCGGTTGCTGGCGCATCGTTCTCTCGCTTCGCTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCgg                                                                                                                           <  1:9857/149‑1 (MQ=255)
                            ctgcggggCTCGCCCGGCGGGCGGGTGCTGGCGCATCGTTATCTCGCTTCGCTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGt                                                                                                                          <  2:771535/143‑1 (MQ=17)
                               cGTGGCTCGCCCGGCGGGCCGTTGCTGGCGCAACGTTCTCTCGCTTCGCTCGAGTCGAACCTCCCTGCCCCCGGCGGTTCTCATCCTCATGAGCGGCAGCTGCAAAAAAGCCTGCTCGTTGAGCAGGCGTTTCGAATTTGGTCGGTGAt                                                                                                                       >  2:730052/1‑149 (MQ=21)
                                     tCGCCCTGCGGGCCGTTGCTGGCGCAACGTTCTCTCGCTTCGCTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATGCGCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCCGGCTTGTCGAATTTGGTCGGTGATAGAGGa                                                                                                                 >  1:415858/1‑149 (MQ=255)
                                      cGCTCTTCGGGGCGTTGCTGTAGTATGGTTCTCTCGCTTCGGTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGAt                                                                                                                <  2:243551/149‑1 (MQ=14)
                                       ttggtGCGGGCCGTTGCTGGCGCATTGTTATCTCGCTTCGGTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGAtt                                                                                                               <  2:1022862/145‑1 (MQ=17)
                                         tttGCGGTCCGGTGGGGGCGCACGGTTCTCTCGCTTCGCTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTCg                                                                                                             <  2:339996/147‑1 (MQ=14)
                                         ccTGCGGGCCGTTGCTGGCGCAACGTTCTCTCGCTTCGCTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATGCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGAttag                                                                                                             >  1:516321/1‑147 (MQ=255)
                                         ccTGCGGGCCGTTGCTGGCGCAACGTTCTCTCGCTTCGCTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCGCCTCCTCAGGAGCGGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGGATTTGGTCGGTGATAGAGGATTCg                                                                                                             >  1:511999/1‑149 (MQ=18)
                                          cTGCGGGCCGTTGCTGGCGCAACGTTCTCTCGCTTCGCTCGAGTCGCACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGGAGATGCAAAAAAGCCTGCTCGTTGAGCAGGGGTTTCGATTTTGGGGGGGGATTGAGGATTGGa                                                                                                            >  1:242205/1‑149 (MQ=18)
                                          cTGCGGGCCGTTGCTGGCGCAACGTTCTCTCGCTTCGCTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTCGa                                                                                                            >  1:493723/1‑149 (MQ=255)
                                            gCGGGCCGTTGCTGGCGCAACGTTCTCTCGCTTCGATCGAGTCGAACCTCCCGGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTCGAAc                                                                                                          <  1:504282/149‑1 (MQ=255)
                                                gCCGTTGCTGGCGCAACGTTCTCTCGCTTCGCTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCAGGAGCTGCAGATGCAAAAAAGCCTGCGCGTTGAGCAGGCGTTTCGCATTTGGTCGGGGATAGAGGAGTCGAGCCTcc                                                                                                      >  1:693184/1‑149 (MQ=18)
                                                   gTTGCGGGCGCAACGTTATCTCGCTTCGCTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTCGAACCTCCGAc                                                                                                   <  1:818001/149‑1 (MQ=255)
                                                      gCTGGCGCAACGTTCTCTCGCTTCGGTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTCGAACCTCCGAcccc                                                                                                <  1:422211/149‑1 (MQ=255)
                                                       cTGGCGCAACGTTCTCTCGCTTCGCTCGAGTCGAACCTCCCTGCCCCCGGCGGTTCTGATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTCGAACATACGACCCCt                                                                                               >  1:9099/1‑149 (MQ=255)
                                                        tGGCGCAACGTTCTCTCTCTTCGCTCGAGTCGAACCTCACTTCACCCGGAGGTTCTCATCCTCATGAGCTGCAGATGAAAAAAAGCCTGGTCTTTGAGCGCGCGTTTGGAATTTGGTGGGTGATAGAGGATTAGAACCTCCGACCCCtg                                                                                              >  2:560980/1‑148 (MQ=17)
                                                          gcgcAACGTTCTCTCGCTTCGCTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTCGACCCTCCGACCCCTTCg                                                                                            >  1:742043/1‑149 (MQ=255)
                                                           cgcAACGTTCTCTCGCTTCGCTCGATTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTCGAACCTCCGACCCCTTCGt                                                                                           <  1:897820/149‑1 (MQ=255)
                                                             gaaCGTTCTCTCGCTGCTCTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTCGAACCTCCGACCCCTTCGTcc                                                                                         <  1:233813/148‑1 (MQ=255)
                                                             cAACGTTCTCTCGCTTCGCTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGt                                                                                                                              >  2:406181/1‑112 (MQ=255)
                                                              atcggtatctcGCGTCGCTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGATGCAAAACAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTCGAACCTCCGACCCCTTCGTccc                                                                                        <  2:573641/142‑1 (MQ=255)
                                                              aaCGTTCACACGCTGCGCTCGAGTCGAACCTCCCTGCCCCCGGACGTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTCGAACCTCCGACCCCTTCGTccc                                                                                        <  1:685227/149‑1 (MQ=255)
                                                                  ttCTCTCGCTTCGCTCGAGTCGCACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGGTGAGCCGGCTTTTCGAATTTGGTCGGTGATAGAGGATTAGAACCTCCGACCCCTTCGTCCcgaac                                                                                    >  2:429365/1‑149 (MQ=255)
                                                                  ttCTCTCGCTTCGCTCGAGTCGAACCTCCGTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTCGAACCTCCGACCCCTTCGTCCcgaac                                                                                    <  2:339345/149‑1 (MQ=255)
                                                                    ctctcGCTTCGCTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCCACCTCATGAGCTCCACATGCAAAAAAGCCTGCTCGTTGAGCAGGGTTTTCGAATTTGGTCGGGGATAGAGGATTCGAACCTCCGCCCCCTTCGTCCcgtacga                                                                                  >  2:809382/1‑149 (MQ=18)
                                                                    ctctcGCTTCGCTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGATGCTAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGATTTTGGTCGGTGATAGAGGATTCGCACCTCCGACCCCTTCGTCCcgaacga                                                                                  >  1:835410/1‑149 (MQ=255)
                                                                      ctcGCTTCGCTCGAGTCGAACCTCCCTGCCCCGGGAGGTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTCGAACCTCCGACCCCTTCGTCCCGAACGAAg                                                                                <  2:191931/149‑1 (MQ=255)
                                                                       tcgcttcgctCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGGAGATGCAAAAAAGCCTGCGCGATGAGCAGGCGTTTCGAATTTGGTCGGTGATAGAGGATTCGAACCTCCGACCCCTGCGTCCCGAACGAAGt                                                                               >  1:776856/1‑149 (MQ=18)
                                                                       tcgctgcgctCGTGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTAATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTTATAGAGGATTCGAACCTCCGACCCCTTCGTCCCGAACGAAGt                                                                               <  2:756536/149‑1 (MQ=255)
                                                                        cgcttcgctCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTCGAACCTCCGACCCCTTCGTCCCGAACGAAGTg                                                                              <  1:852337/149‑1 (MQ=255)
                                                                         gcttcgctCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGATTTTGGTCGGTGATAGAGGATTCGAACCTCCGACCCCTTCGTCCCGAACGAAGTgc                                                                             <  2:506153/149‑1 (MQ=255)
                                                                           ttcgctCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTGGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTCGAACCTCCGACCCCTTCGTCCCGAACGAAGTgcgc                                                                           >  1:128787/1‑149 (MQ=255)
                                                                              gctCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTCGAACCTCCGACCCCTTCGTCCCGAACGAAGTGCGCTAc                                                                        <  2:820994/149‑1 (MQ=255)
                                                                                tgctgtggAACTTCCCTGCCCCCGTAGGTTCTCATCCTCATGAGCTGAAGATGCTAAAACGCCTGCTCGTGGAGCAGGCTTTTCTAATTTGGTCGGTGATAGAGGATTCGAACCTCCGACCCCTTCGTCCCGAACGAAGTGCGCTACCa                                                                      <  2:710225/142‑1 (MQ=18)
                                                                                tCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTCGAACCTCCGACCCCTTCGTCCCGAACGAAGTGCGCTAccc                                                                      >  1:1009229/1‑148 (MQ=255)
                                                                                tCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTCGAACCTCCGACCCCTTCGTCCCGAACGAAGTGCGCTACCa                                                                      >  1:962803/1‑149 (MQ=255)
                                                                                 ggtgTCGAACATCCCTGCCCCCGGAGTTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCTTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTCGAACCTCCGACCCCTTCGTCCCGAACGAAGTGCGCTACGAg                                                                     <  2:178289/146‑1 (MQ=18)
                                                                                  gAGTCGAACGTCCCTTCCCCCGTAGGTTCTCATCCTAATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTCGAACCTCCGACCCCTTCGTCCCGAACGAAGTGCGCTACCAgg                                                                    <  2:583086/149‑1 (MQ=255)
                                                                                  gAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTCGAACCTCCGACCCCTTCGTCCCGAACGAAGTGCGCTACCAgg                                                                    >  1:305899/1‑149 (MQ=255)
                                                                                    gTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTCGAACCTCCGACCCCTTAGTCCCGAACGAAGTGCGCTACCAGGCt                                                                  >  2:4269/1‑149 (MQ=255)
                                                                                     tCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTCGAACCTCCGACCCCTTCGTCCCGAAAGAAGTGCGCTACCAGGCTg                                                                 >  2:329440/1‑149 (MQ=255)
                                                                                           cTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTCGAACCTCCGACCCCTTCGTCCCGAACGAAGTGCGCTACCAGGCTGCGCCaa                                                           <  2:723260/149‑1 (MQ=255)
                                                                                             cccTGCCCCCGGCGGTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTCGCACCTCCGACCCCTTCGTCCCGCACGAAGTGCGCTACCAGGCGGCGCCACTc                                                         >  1:175009/1‑149 (MQ=18)
                                                                                             cccTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTCGAACCTCCGCCCCCTTCGTCCCGAACGACGTGCGCTACCAGGCTGCGCCAATc                                                         >  1:576383/1‑149 (MQ=255)
                                                                                             cccTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTCGAACCTCCGACCCCTTCGTCCCGAACGAAGTGCGCTACCAGGCTGCGCCAATc                                                         >  2:10494/1‑149 (MQ=255)
                                                                                              ccTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGTTGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTCGAACATCCGCCCCCTTCGTCCCGAACGAAGTGCGCTACCAGGCTGCGCCAAtga                                                        >  2:828041/1‑147 (MQ=255)
                                                                                               cTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTCGAACCTCCGACCCCTTCGTCCCGAACGAAGTGCGCTACCAGGCTGCGCCAATCAc                                                       <  1:164365/149‑1 (MQ=255)
                                                                                                tGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTCGAACCTCCGACCCCTTCGTCCCGAACGAAGTGCGCTACCAGGCTGCGCCAATCAcc                                                      <  2:247436/149‑1 (MQ=255)
                                                                                                   ccccGGAGGTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGATCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTCGAACCTCCGACCCCATCGTCACGAACCAAGTGCGCTACCACGCTGCGCCAATCACCGaa                                                   >  1:796807/1‑149 (MQ=21)
                                                                                                     ccGGAGGTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTCGAACCTCCGACCCCTTCGTCCCGAACGAAGTGCGCTACCAGGCTGCGCCAATCACCGAATg                                                 >  1:1022410/1‑149 (MQ=255)
                                                                                                         cgggtctcATCCTCATGAGCGGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTCGAACCTCCGACCCCTTCGTCCCGAACGAAGTGCGCTACCAGGCTGCGCCAATCACCGAATGCggg                                             <  1:645301/145‑1 (MQ=255)
                                                                                                         aGGTTCTCATCCTCATTAGCTGCAGATGCAAAAAAGCCTGCTCGATCAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTCGAACCTCCGACCCCTTCGTCCCGAACGAAGTGCGCTACCAGGCTGCGCCAATCACCGAATGCggg                                             <  2:373641/149‑1 (MQ=255)
                                                                                                           gTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTCGCACCTCCGACCCCTTCGTCCCGAACGAAGTGCGCTACCAGGCTGCGCCAATCACCGAATGCGGGgc                                           >  2:211905/1‑149 (MQ=255)
                                                                                                           gTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTCGAACCTCCGACCCCTTCGTCCCGAACGAAGTGCGCTACCAGGCTGCGCCAATCACCGAATGCGGGgc                                           >  2:28832/1‑149 (MQ=255)
                                                                                                           gTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTCGAACCTCCGACCCCTTCGTCCCGAACGAAGTGCGCTACCAGGCTGCGCCAATCACCGAATGCGGGgc                                           >  1:999931/1‑149 (MQ=255)
                                                                                                                 aTCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTCGAACCTCCGCCCCCTTCGTCCCCAACGAAGAGCGCTACCAGCCTGCGCCAATCACCGAATGCGGGGCGCAACt                                     >  1:1010942/1‑149 (MQ=25)
                                                                                                                 aTCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTCGAACCTCCGACCCCTTCGTCCCGAACGAAGTGCGCTACCAGGCTGCGCCAATCACCGAATGCGGGGCGCATCt                                     <  1:352462/149‑1 (MQ=255)
                                                                                                                   ccTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTCGAACCTCCGACCCCTTCGTCCCGAACGAAGTGCGCTACCAGGCTGCGCCAATCACCGAATGCGGGGCGCATCTTa                                   >  2:922520/1‑149 (MQ=255)
                                                                                                                      cATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTCGAACCTCCGACCCCTTCGTCCCGAACGAAGTGCGCTACCAGGCTGCGCCAATCACCGAATGCGGGGCGCATCTTACTg                                >  1:878339/1‑149 (MQ=255)
                                                                                                                       aTGAGCTGCAGATGCAAAAAATCCTGCTCGTTGAGCAGGCTTATCGAATTTTGTCGGTGATAGAGGATTCGAACCTCCGACCCCTTCGTCACGAACGAAGTGCGCTACCAGGCGGCGCCAATCACCGAATGCGAGGCGCATCTTACTgc                               >  2:190545/1‑149 (MQ=37)
                                                                                                                       aTGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTCGAACCTCCGACCCCTTCGTCCCGAACGAAGTGCGCTACCAGGCTGCGCCAATCACCGAATGCGGGGCGCATCTTACTgc                               <  1:206903/149‑1 (MQ=255)
                                                                                                                       aTGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTCGAACCTCCGACCCCTTCGTCCCGAACGAAGTGCGCTACCAGGCTGCGCCAATCACAGAATGCGGGGCGCATCTTACTgc                               >  1:797641/1‑149 (MQ=255)
                                                                                                                               cAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTCGAACCTCCGACCCCTTCGTCCCGAACGAAGTGCGCTACCAGGCTGCGCCAATCACCGAATGCGGGGCGCATCTTACTGCGCAGATAc                       >  1:27463/1‑149 (MQ=255)
                                                                                                                                  aTGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTCGAACCTCAGACCCCTTCGTACCGAACGAAGTGCGCTCCCAGGCTGCGCCAATCACCGAATGCGGGGCGCATCTTACTGCGCAGATACGcc                    >  2:383367/1‑149 (MQ=255)
                                                                                                                                    gCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTCGAACCTCCGACCCCTTCGTCCCGAACGAAGTGCGCTACCAGGCTGCGCCAATCACCGAATGCGGGGCGCATCTTACTGCGCAGATACGCCCt                  >  2:436480/1‑149 (MQ=255)
                                                                                                                                      aaaaaaGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTCGAACCTCCGACCCCTTCGTCCCGAACGAAGTGCGCTACCAGGCTGCGCCAATCACCGAATGCGGGGCGCATCTTACTGCGCAGATACGCCCTCg                >  1:39626/1‑149 (MQ=255)
                                                                                                                                       aaaaaGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTCGAACCTCCGCCCCCTTCGTCCCGAACGAAGTGCGCTACCAGGCTGCGCCAATCACCGAATGCGGGGCGCATCTTACTGCGCAGATACGCCCTCGt               >  1:933213/1‑149 (MQ=255)
                                                                                                                                       aaaaaGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTCGAACCTCCGACCCCTTCGTCCCGAACGAAGTGCGCTACCAGGCTGCGCCAATCACCGAATGCGGGGCGCATCTTACTGCGCAGATACGCCCTCGt               <  1:253132/149‑1 (MQ=255)
                                                                                                                                        aaacGCCTGCGCGTGGAGCAGGCTTTTCGAATATGGTCGGTGATAGAGGATTCGAACCTCCGACCCCTTCGTCCCGAACGAAGTGCGCTACCAGGCTGCGCCAATCACCGAATGCGGGGCGCATCTTACTGCGCAGATACGCCCTCGTc              <  2:945465/149‑1 (MQ=255)
                                                                                                                                        aaaaGCCTGCTCAACGAGCAGACTGTTCGAAGTTGGTCGGTGATAGAGGATTCGAGCCTCCGACCCCTTCGACCCGCACGCAGTGCGCTACCAGGCTGCGCCAATCACCGCATGCGGGGCGCATCTTACTGCGCAGATACGCCCTCggc              >  2:215197/1‑147 (MQ=255)
                                                                                                                                          aaGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTCGAACCTCCGACCCCTTCGTCCCGAACGAAGTGCGCTACCAGGCTGCGCCACTCACCGAATGCCGGGCGCCTCGTACTGCGCGGTAACGCCCTCGTCaa            >  1:988948/1‑149 (MQ=37)
                                                                                                                                            gCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTCGAACCTCCGACCCCTTCGTCCCGAACGAAGTGCGCTACCAGGCTGCGCCAATCACCGAATGCGGGGCGCATCTTACTGCGCAGATACGCCCTCGTCAGTc          <  2:321358/149‑1 (MQ=255)
                                                                                                                                            gCCTGCTCAACGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTCGAACCTCCGACCCCt                                                                                               >  2:603939/1‑64 (MQ=18)
                                                                                                                                            gCCTGCTCAACGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTCGAACCTCCGACCCCt                                                                                               <  1:603939/64‑1 (MQ=18)
                                                                                                                                            gCCTGCTCAACGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTCGAACCTCCGACCCCt                                                                                               <  1:221262/64‑1 (MQ=18)
                                                                                                                                             ccTGCTCAACGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTCGAACCTCCGACCCCTTCGTCCCGAACGAAGTGCGCTACCAGGCTGCGCCAATCACCGAATGCGGGGCGCATCTTACTGCGCAGATACGCCCTCGTCAATcc         >  1:684589/1‑149 (MQ=255)
                                                                                                                                              cTGCTCGTTGAGCAGGCTTTTCTAATTTGGTCGGTGATTGAGGATTCGAACCTCCGACCCCATCGTCCCGTACGAAGTGCGCTACCAGGCTGCGCCAAGCACCGAATGCGGGGCGCATCTTACTGCGCAGATACGCCCTCGTCAATccc        <  2:966234/149‑1 (MQ=39)
                                                                                                                                                   cGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTCGAACCTCCGACCCCTTCGTCCCGAACGAAGTGCGCTACCAGGCTGCGCCAATCACCGAATGCGGGGCGCATCTTACTGCGCAGATACGCCCTCGTCAATCCCTTAAt   <  1:417261/149‑1 (MQ=255)
                                                                                                                                                   cGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTCGAACCTCCGACCCCTTCGTCCCGAACGAAGTGCGCTACCAGGCTGCGCCAATCACCGAATGCGGGGCGCATCTTACTGCGCAGATACGCCCTCGTCAATCCCTTAAt   <  2:653100/149‑1 (MQ=255)
                                                                                                                                                    gTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTCGAACCTCCGACCCCTTCGTCCCGAACGAAGTGCGCTACCAGGCTGCGCCAATCACCGAATGCGGGGCGCATCTTACTGCGCAGATACGCCCTCGTCAATCCCTTAATa  <  2:536236/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
TTGGTCGGTGATAGAGGATTACTCGCCACTTCGTGGCTCGCCCTGCGGGCCGTTGCTGGCGCAACGTTCTCTCGCTTCGCTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTCGAACCTCCGACCCCTTCGTCCCGAACGAAGTGCGCTACCAGGCTGCGCCAATCACCGAATGCGGGGCGCATCTTACTGCGCAGATACGCCCTCGTCAATCCCTTAATA  >  NC_000913/3708447‑3708743

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: