Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,708,596 T→A 6.3% intergenic (‑891/+20) dppA ← / ← proK dipeptide/heme ABC transporter periplasmic binding protein; dipeptide chemotaxis receptor/tRNA‑Pro

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,708,5960TA 6.3% 376.7 / 11.1 179intergenic (‑891/+20)dppA/proKdipeptide/heme ABC transporter periplasmic binding protein; dipeptide chemotaxis receptor/tRNA‑Pro
Reads supporting (aligned to +/- strand):  ref base T (87/72);  new base A (6/6);  total (100/79)
Fisher's exact test for biased strand distribution p-value = 7.73e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.70e-01

Only 100 of 180 total aligned reads displayed.

TCGGTGATAGAGGATTACTCGCCACTTCGTGGCTCGCCCTGCGGGCCGTTGCTGGCGCAACGTTCTCTCGCTTCGCTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTCGAACCTCCGACCCCTTCGTCCCGAACGAAGTGCGCTACCAGGCTGCGCCAATCACCGAATGCGGGGCGCATCTTACTGCGCAGATACGCCCTCGTCAATCCCTTAATAG  >  NC_000913/3708451‑3708744
                                                                                                                                                 |                                                                                                                                                    
tCGGTGATAGCGGAGTACTCGCCACTTCGTGGCTCGCCCTGCGGGCCGGTGCTGGCGGATCGTTCTCTCGCTTCGCTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGa                                                                                                                                                   <  1:63386/149‑1 (MQ=25)
tCGGTGATAGAGGATTACTCGCCACTTGGGGGCACGCCCTGCGGGGCGTTGCGGGCTGAACGTTATCTCGCCTCGGTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGa                                                                                                                                                   <  1:955762/149‑1 (MQ=18)
    tGATAGAGGATTACTCGCCACGTCGTGGCTCGCCCTGCGGGCCGTTGCTGGCGCAACGTTCTCTCGCTTCGCTCGAGTCGAACCTCCCTGCCCCCGGCGGGTCTCAGACGCATGCGCTGCAGATGCAAAAAAGCCTGCGCGGTGAGccg                                                                                                                                               >  1:373607/1‑147 (MQ=37)
     taTAGAGGATTAAACGCCACTTGGTGGCTCTCATTGCGGTACGTAGCTGGCGCAACGTTCTCTCGCTTAGCTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAgg                                                                                                                                              <  2:641013/148‑1 (MQ=18)
      aTAGAGGATTACTCGCCACTTCGTGGCTCGCCCTGCGGGCCGTTGCTGGCGCAACGTTCTCTCGCTTCGCTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCAGGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGc                                                                                                                                             >  1:975811/1‑149 (MQ=255)
        agagGATTCCTCGCCTCTGCGTGGCTCGCCCTGCGGGCCGTTGAGGGCGCAACGTTCTCTCGCTTCGCTCGCGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCATATGCAAAAAAGCCTGCTCGTTGAGCAGGCtt                                                                                                                                           <  2:373607/149‑1 (MQ=21)
        agagGATTACTCGCCACTTCGTGGCTCGCCCTGCGGGCCGTTGCTGGCGCAACGTTCTCTCGCTTCGCTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCAACGAGCAGGCtt                                                                                                                                           <  2:537376/149‑1 (MQ=255)
          agGATTACTCGCCACTTCGTGGCTCGCCCTGCGGGCCGTTGCTGGCGCAACGTTCTCTCGCTTCGCTCGAGTCGCGCCTCCCTGCCCCCGGCGGTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCGCGGTGAGCCGGCtttt                                                                                                                                         >  1:175807/1‑149 (MQ=25)
            gATTACTCGCCACTTCGTGGCTCGCCCTGCGGGCCGTTGCTGGCGCAACGTTCTCTCGCTTCGCTCGAGTCGCACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCg                                                                                                                                       >  1:723260/1‑149 (MQ=255)
            gATTACTCGCCACTTCGTGGCTCGCCCTGCGGGCCGTTGCTGGCGCAACGTTCTCTCGCTTCGCTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCg                                                                                                                                       >  2:178760/1‑149 (MQ=255)
            ccctACTCGCCACTTCGTGGCTCGCCCAGCGGGCCGTCGCTGGCGCAACGTTCTCTCGCTTCGCTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCAACGAGCAGGCtttttt                                                                                                                                       <  1:425438/146‑3 (MQ=21)
                 cTCGCCACTTCGTGGCTCGCCCTGCGGGCCGTTGCTGGCGCAACGTTCTCTCGCTTCGCTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAAttt                                                                                                                                  <  1:62989/149‑1 (MQ=255)
                 cTCGCCACTTCGTGGCTCGCCCTGCGGGCCGTTGCTGGCGCAACGTTCTCTCGCTTCGCTCGAGTCGAACCTCCCGGCCCCCGGAGGTTCGCAGCCTCATGAGCTGCAGCTGGAAAAAAGCCTGCGCGTTGGGCAGGGGGTTCGAAttt                                                                                                                                  >  1:618090/1‑149 (MQ=255)
                  tCGCCACTTCGTGGCTCGCCCTGCGGGCCGTTGCTGGCGCACCGTTCTCTCGCTTCGCTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATCAGCTGCAGATGCAAAAAAGCCTGCACGTTGAGCAGGATTTTCGAAtttt                                                                                                                                 >  2:331756/1‑148 (MQ=255)
                  tCGCCACTTCGTGGCTCGCCCTGCGGGCCGTTGCTGGCGCAACGTTCTCTCGCTTCGCTCGAGTCGCACCTCCCTGCCCCCGGAGGTTCTCAGCCGCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCGTTTCGAATTTg                                                                                                                                 >  2:74394/1‑149 (MQ=255)
                  tCGCCACTTCGTGGCTCGCCCTGCGGGCCGTTGCTGGCGCAACGTTCTCTCGCTTCGCTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGATGCAAAAAGGCCTGCTCGGGGAGCAGGCGTTTCGAATTTg                                                                                                                                 >  1:509297/1‑149 (MQ=255)
                  tCGCCACTTCGTGGCTCGCCCTGCGGGCCGTTGCTGGCGCAACGTTCTCTCGCTTCGCTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCAGCCTCATGAGCTGCCGCTGCAAAAAAGCCTGCTCGTGGCGCAGGCTTTTCGAATTTg                                                                                                                                 >  1:331066/1‑149 (MQ=25)
                    gCCACTTCGTGGCTCGCCCTGCGGGCCGTTGCTGGCGCAACGTTCTCTCGCTTCGCTCGAGTCGAACCTCCCTGCCCCCGGCGGTTCTCATCCTCATGAGCTGGAGATGCAAAAAAGCCTGCTCGTAGAGCAGGCTTTTCGAATTTGGt                                                                                                                               >  1:627273/1‑149 (MQ=255)
                       aCTTCGTGGCTCGCCCTGCGGGCGGTTGCTGGCGCATCGTTCTCTCGCTTCGCTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCgg                                                                                                                            <  1:9857/149‑1 (MQ=255)
                        ctgcggggCTCGCCCGGCGGGCGGGTGCTGGCGCATCGTTATCTCGCTTCGCTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGt                                                                                                                           <  2:771535/143‑1 (MQ=17)
                           cGTGGCTCGCCCGGCGGGCCGTTGCTGGCGCAACGTTCTCTCGCTTCGCTCGAGTCGAACCTCCCTGCCCCCGGCGGTTCTCATCCTCATGAGCGGCAGCTGCAAAAAAGCCTGCTCGTTGAGCAGGCGTTTCGAATTTGGTCGGTGAt                                                                                                                        >  2:730052/1‑149 (MQ=21)
                             tGGCTCGCCCTGCGGGCCGTTGCTGGCGCAACGTTCTCTCGCTTCGCTCGCGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATaa                                                                                                                      >  2:209916/1‑148 (MQ=255)
                                 tCGCCCTGCGGGCCGTTGCTGGCGCAACGTTCTCTCGCTTCGCTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATGCGCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCCGGCTTGTCGAATTTGGTCGGTGATAGAGGa                                                                                                                  >  1:415858/1‑149 (MQ=255)
                                   ttggtGCGGGCCGTTGCTGGCGCATTGTTATCTCGCTTCGGTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGAtt                                                                                                                <  2:1022862/145‑1 (MQ=17)
                                     tttGCGGTCCGGTGGGGGCGCACGGTTCTCTCGCTTCGCTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTCg                                                                                                              <  2:339996/147‑1 (MQ=14)
                                     ccTGCGGGCCGTTGCTGGCGCAACGTTCTCTCGCTTCGCTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATGCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGAttag                                                                                                              >  1:516321/1‑147 (MQ=255)
                                     ccTGCGGGCCGTTGCTGGCGCAACGTTCTCTCGCTTCGCTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCGCCTCCTCAGGAGCGGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGGATTTGGTCGGTGATAGAGGATTCg                                                                                                              >  1:511999/1‑149 (MQ=18)
                                      cTGCGGGCCGTTGCTGGCGCAACGTTCTCTCGCTTCGCTCGAGTCGCACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGGAGATGCAAAAAAGCCTGCTCGTTGAGCAGGGGTTTCGATTTTGGGGGGGGATTGAGGATTGGa                                                                                                             >  1:242205/1‑149 (MQ=18)
                                      cTGCGGGCCGTTGCTGGCGCAACGTTCTCTCGCTTCGCTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTCGa                                                                                                             >  1:493723/1‑149 (MQ=255)
                                        gCGGGCCGTTGCTGGCGCAACGTTCTCTCGCTTCGATCGAGTCGAACCTCCCGGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTCGAAc                                                                                                           <  1:504282/149‑1 (MQ=255)
                                            gCCGTTGCTGGCGCAACGTTCTCTCGCTTCGCTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCAGGAGCTGCAGATGCAAAAAAGCCTGCGCGTTGAGCAGGCGTTTCGCATTTGGTCGGGGATAGAGGAGTCGAGCCTcc                                                                                                       >  1:693184/1‑149 (MQ=18)
                                             ccGTTGCTGGCGCAACGTTCTCTCGCTTCGCTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTGGAGCAGGCTTTTCGAGTTTGGTCGGTGATAGAGGATTCGCAccgccg                                                                                                      >  2:123843/1‑149 (MQ=255)
                                               gTTGCGGGCGCAACGTTATCTCGCTTCGCTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTCGAACCTCCGAc                                                                                                    <  1:818001/149‑1 (MQ=255)
                                                 tGCTGGCGCAACGTTCTCTCGCTTCGCTCGAGTCGAACCTCACTGCCCCCGGAGGTTCTCATCCTCAACAGCTGCACATTCAAAAAAGCCTGAAAGTTGAGCAGGGTTTTCTAATTTGGGAGGGGATAGAGGATTCGATCCTACGAccc                                                                                                  >  2:574452/1‑149 (MQ=18)
                                                  gCTGGCGCAACGTTCTCTCGCTTCGGTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTCGAACCTCCGAcccc                                                                                                 <  1:422211/149‑1 (MQ=255)
                                                   cTGGCGCAACGTTCTCTCGCTTCGCTCGAGTCGAACCTCCCTGCCCCCGGCGGTTCTGATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTCGAACATACGACCCCt                                                                                                >  1:9099/1‑149 (MQ=255)
                                                    tGGCGCAACGTTCTCTCTCTTCGCTCGAGTCGAACCTCACTTCACCCGGAGGTTCTCATCCTCATGAGCTGCAGATGAAAAAAAGCCTGGTCTTTGAGCGCGCGTTTGGAATTTGGTGGGTGATAGAGGATTAGAACCTCCGACCCCtg                                                                                               >  2:560980/1‑148 (MQ=17)
                                                      gcgcAACGTTCTCTCGCTTCGCTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTCGACCCTCCGACCCCTTCg                                                                                             >  1:742043/1‑149 (MQ=255)
                                                       cgcAACGTTCTCTCGCTTCGCTCGATTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTCGAACCTCCGACCCCTTCGt                                                                                            <  1:897820/149‑1 (MQ=255)
                                                       cgcAACGTGCTCTCGCTTCGCTCGCGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTCGAACCTCCGACCCCTTCGt                                                                                            <  2:212361/149‑1 (MQ=255)
                                                        gcAACGTTCTCTCGCTTCGCTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTCGAACCTCCGACCCCTTCGTc                                                                                           >  2:416253/1‑149 (MQ=255)
                                                         gaaCGTTCTCTCGCTGCTCTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTCGAACCTCCGACCCCTTCGTcc                                                                                          <  1:233813/148‑1 (MQ=255)
                                                          aaCGTTCACACGCTGCGCTCGAGTCGAACCTCCCTGCCCCCGGACGTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTCGAACCTCCGACCCCTTCGTccc                                                                                         <  1:685227/149‑1 (MQ=255)
                                                              ttCTCTCGCTTCGCTCGAGTCGCACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGGTGAGCCGGCTTTTCGAATTTGGTCGGTGATAGAGGATTAGAACCTCCGACCCCTTCGTCCcgaac                                                                                     >  2:429365/1‑149 (MQ=255)
                                                                ctctcGCTTCGCTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCCACCTCATGAGCTCCACATGCAAAAAAGCCTGCTCGTTGAGCAGGGTTTTCGAATTTGGTCGGGGATAGAGGATTCGAACCTCCGCCCCCTTCGTCCcgtacga                                                                                   >  2:809382/1‑149 (MQ=18)
                                                                ctctcGCTTCGCTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGATGCTAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGATTTTGGTCGGTGATAGAGGATTCGCACCTCCGACCCCTTCGTCCcgaacga                                                                                   >  1:835410/1‑149 (MQ=255)
                                                                 tctcGCTTAGGTCGAGGCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGGTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTCGAACCTCCGACCCCTTCGTCCcgaacgaa                                                                                  <  2:475326/149‑1 (MQ=255)
                                                                  ctcGCTTCGCTCGAGTCGAACCTCCCTGCCCCGGGAGGTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTCGAACCTCCGACCCCTTCGTCCCGAACGAAg                                                                                 <  2:191931/149‑1 (MQ=255)
                                                                   tcgcttcgctCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGGAGATGCAAAAAAGCCTGCGCGATGAGCAGGCGTTTCGAATTTGGTCGGTGATAGAGGATTCGAACCTCCGACCCCTGCGTCCCGAACGAAGt                                                                                >  1:776856/1‑149 (MQ=18)
                                                                   tcgctgcgctCGTGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTAATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTTATAGAGGATTCGAACCTCCGACCCCTTCGTCCCGAACGAAGt                                                                                <  2:756536/149‑1 (MQ=255)
                                                                    cgcttcgctCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTCGAACCTCCGACCCCTTCGTCCCGAACGAAGTg                                                                               <  1:852337/149‑1 (MQ=255)
                                                                       ttcgctCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTGGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTCGAACCTCCGACCCCTTCGTCCCGAACGAAGTgcgc                                                                            >  1:128787/1‑149 (MQ=255)
                                                                          gctCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTCGAACCTCCGACCCCTTCGTCCCGAACGAAGTGCGCTAc                                                                         <  2:820994/149‑1 (MQ=255)
                                                                            tgctgtggAACTTCCCTGCCCCCGTAGGTTCTCATCCTCATGAGCTGAAGATGCTAAAACGCCTGCTCGTGGAGCAGGCTTTTCTAATTTGGTCGGTGATAGAGGATTCGAACCTCCGACCCCTTCGTCCCGAACGAAGTGCGCTACCa                                                                       <  2:710225/142‑1 (MQ=18)
                                                                            tCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTCGAACCTCCGACCCCTTCGTCCCGAACGAAGTGCGCTAccc                                                                       >  1:1009229/1‑148 (MQ=255)
                                                                            tCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTCGAACCTCCGACCCCTTCGTCCCGAACGAAGTGCGCTACCa                                                                       >  1:962803/1‑149 (MQ=255)
                                                                              gAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTCGAACCTCCGACCCCTTCGTCCCGAACGAAGTGCGCTACCAgg                                                                     >  1:305899/1‑149 (MQ=255)
                                                                                   gAACCTCCCTGCCCCCGGAGGTTCTCAGCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTCGAACCTCCGACCCCTTCGTCCCGCACGAAGTGCGCTACCAGGCTggg                                                                >  2:328995/1‑147 (MQ=255)
                                                                                       cTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTCGAACCTCCGACCCCTTCGTCCCGAACGAAGTGCGCTACCAGGCTGCGCCaa                                                            <  2:723260/149‑1 (MQ=255)
                                                                                         cccTGCCCCCGGCGGTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTCGCACCTCCGACCCCTTCGTCCCGCACGAAGTGCGCTACCAGGCGGCGCCACTc                                                          >  1:175009/1‑149 (MQ=18)
                                                                                         cccTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTCGAACCTCCGCCCCCTTCGTCCCGAACGACGTGCGCTACCAGGCTGCGCCAATc                                                          >  1:576383/1‑149 (MQ=255)
                                                                                         cccTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTCGAACCTCCGACCCCTTCGTCCCGAACGAAGTGCGCTACCAGGCTGCGCCAATc                                                          >  2:10494/1‑149 (MQ=255)
                                                                                          ccTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGTTGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTCGAACATCCGCCCCCTTCGTCCCGAACGAAGTGCGCTACCAGGCTGCGCCAAtga                                                         >  2:828041/1‑147 (MQ=255)
                                                                                           cTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTCGAACCTCCGACCCCTTCGTCCCGAACGAAGTGCGCTACCAGGCTGCGCCAATCAc                                                        <  1:164365/149‑1 (MQ=255)
                                                                                            tGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTCGAACCTCCGACCCCTTCGTCCCGAACGAAGTGCGCTACCAGGCTGCGCCAATCAcc                                                       <  2:243762/149‑1 (MQ=255)
                                                                                               ccccGGAGGTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGATCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTCGAACCTCCGACCCCATCGTCACGAACCAAGTGCGCTACCACGCTGCGCCAATCACCGaa                                                    >  1:796807/1‑149 (MQ=21)
                                                                                                 ccGGAGGTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTc                                                                                                               >  2:569352/1‑88 (MQ=31)
                                                                                                 ccGGAGGTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTCGAACCTCCGACCCCTTCGTCCCGAACGAAGTGCGCTACCAGGCTGCGCCAATCACCGAATg                                                  >  1:1022410/1‑149 (MQ=255)
                                                                                                     cgggtctcATCCTCATGAGCGGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTCGAACCTCCGACCCCTTCGTCCCGAACGAAGTGCGCTACCAGGCTGCGCCAATCACCGAATGCggg                                              <  1:645301/145‑1 (MQ=255)
                                                                                                       gTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTCGAACCTCCGACCCCTTCGTCCCGAACGAAGTGCGCTACCAGGCTGCGCCAATCACCGAATGCGGGgc                                            >  2:28832/1‑149 (MQ=255)
                                                                                                       gTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTCGAACCTCCGACCCCTTCGTCCCGAACGAAGTGCGCTACCAGGCTGCGCCAATCACCGAATGCGGGgc                                            >  1:999931/1‑149 (MQ=255)
                                                                                                             aTCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTCGAACCTCCGCCCCCTTCGTCCCCAACGAAGAGCGCTACCAGCCTGCGCCAATCACCGAATGCGGGGCGCAACt                                      >  1:1010942/1‑149 (MQ=25)
                                                                                                             aTCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTCGAACCTCCGACCCCTTCGTCCCGAACGAAGTGCGCTACCAGGCTGCGCCAATCACCGAATGCGGGGCGCATCt                                      <  1:352462/149‑1 (MQ=255)
                                                                                                               ccTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTCGAACCTCCGACCCCTTCGTCCCGAACGAAGTGCGCTACCAGGCTGCGCCAATCACCGAATGCGGGGCGCATCTTa                                    >  2:922520/1‑149 (MQ=255)
                                                                                                                 ccctGAGCTGCCGATGCAAAAAAGCCTGCTCGTAGAGCAGGCTTTTCGAATTTGGCCGGTGATAGAGGATTCGAACCTCCGACCCCTTCGTCCCGAACGAAGTGCGCTACCAGGCTGCGCCAATCACCGAATGCGGGGCGCATCTTACt                                  <  2:51801/146‑1 (MQ=255)
                                                                                                                  cATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTCGAACCTCCGACCCCTTCGTCCCGAACGAAGTGCGCTACCAGGCTGCGCCAATCACCGAATGCGGGGCGCATCTTACTg                                 >  1:878339/1‑149 (MQ=255)
                                                                                                                   aTGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTCGAACCTCCGACCCCTTCGTCCCGAACGAAGTGCGCTACCAGGCTGCGCCAATCACCGAATGCGGGGCGCATCTTACTgc                                >  2:380363/1‑149 (MQ=255)
                                                                                                                   aTGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTCGAACCTCCGACCCCTTCGTCCCGAACGAAGTGCGCTACCAGGCTGCGCCAATCACCGAATGCGGGGCGCATCTTACTgc                                <  1:206903/149‑1 (MQ=255)
                                                                                                                   aTGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTCGAACCTCCGACCCCTTCGTCCCGAACGAAGTGCGCTACCAGGCTGCGCCAATCACAGAATGCGGGGCGCATCTTACTgc                                >  1:797641/1‑149 (MQ=255)
                                                                                                                    tGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTCGAACCTCCGACCCCTTCGTCCCGAACGAAGTGCGCTACCAGGCTGCGCCAATCACCGAATGCGGGGCGCATCTTACTgcg                               <  2:420130/149‑1 (MQ=255)
                                                                                                                           cAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTCGAACCTCCGACCCCTTCGTCCCGAACGAAGTGCGCTACCAGGCTGCGCCAATCACCGAATGCGGGGCGCATCTTACTGCGCAGATAc                        >  1:27463/1‑149 (MQ=255)
                                                                                                                                 cAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTCGAACCTCCGACCCCTTCGTCCCGAACGAAGTGCGCTACCAGGCTGCGCCAATCACCGAATGCGGGGCGCATCTTACTGCGCAGATACGCCCTc                  <  2:435666/149‑1 (MQ=255)
                                                                                                                                  aaaaaaGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTCGAACCTCCGACCCCTTCGTCCCGAACGAAGTGCGCTACCAGGCTGCGCCAATCACCGAATGCGGGGCGCATCTTACTGCGCAGATACGCCCTCg                 >  1:39626/1‑149 (MQ=255)
                                                                                                                                   aaaaaGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTCGAACCTCCGCCCCCTTCGTCCCGAACGAAGTGCGCTACCAGGCTGCGCCAATCACCGAATGCGGGGCGCATCTTACTGCGCAGATACGCCCTCGt                >  1:933213/1‑149 (MQ=255)
                                                                                                                                   aaaaaGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTCGAACCTCCGACCCCTTCGTCCCGAACGAAGTGCGCTACCAGGCTGCGCCAATCACCGAATGCGGGGCGCATCTTACTGCGCAGATACGCCCTCGt                <  1:253132/149‑1 (MQ=255)
                                                                                                                                    aaacGCCTGCGCGTGGAGCAGGCTTTTCGAATATGGTCGGTGATAGAGGATTCGAACCTCCGACCCCTTCGTCCCGAACGAAGTGCGCTACCAGGCTGCGCCAATCACCGAATGCGGGGCGCATCTTACTGCGCAGATACGCCCTCGTc               <  2:945465/149‑1 (MQ=255)
                                                                                                                                      aaGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTCGAACCTCCGACCCCTTCGTCCCGAACGAAGTGCGCTACCAGGCTGCGCCACTCACCGAATGCCGGGCGCCTCGTACTGCGCGGTAACGCCCTCGTCaa             >  1:988948/1‑149 (MQ=37)
                                                                                                                                       aGCCTGCTCAACGAGCAGGCTGTTCGAATTTGGTCGGTGATAGAGGATTCGCACCTCCGACCCCTTCGTCCCGAACGAAGTGCGCTACCAGGCTGCGCCAATCACCTAATGCGGGGCGCAACTTACTGCGCAGATACGCCCTCGTCAAt            >  2:391129/1‑149 (MQ=38)
                                                                                                                                        gCCTGCTCAACGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTCGAACCTCCGACCCCt                                                                                                <  1:603939/64‑1 (MQ=18)
                                                                                                                                        gCCTGCTCAACGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTCGAACCTCCGACCCCt                                                                                                <  1:221262/64‑1 (MQ=18)
                                                                                                                                        gCCTGCTCAACGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTCGAACCTCCGACCCCt                                                                                                >  2:221262/1‑64 (MQ=18)
                                                                                                                                         ccTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTCGAACCTCCGACCCCTTCGTCCCGAACGAAGTGCGCTACCAGGCTGCGCCAATCACCGAATGCGGGGCGCATCTTACTGCGCAGATACGCCCTCGTCAATcc          >  2:177545/1‑149 (MQ=255)
                                                                                                                                         ccTGCTCAACGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTCGAACCTCCGACCCCTTCGTCCCGAACGAAGTGCGCTACCAGGCTGCGCCAATCACCGAATGCGGGGCGCATCTTACTGCGCAGATACGCCCTCGTCAATcc          >  1:684589/1‑149 (MQ=255)
                                                                                                                                          cTGCTCGTTGAGCAGGCTTTTCTAATTTGGTCGGTGATTGAGGATTCGAACCTCCGACCCCATCGTCCCGTACGAAGTGCGCTACCAGGCTGCGCCAAGCACCGAATGCGGGGCGCATCTTACTGCGCAGATACGCCCTCGTCAATccc         <  2:966234/149‑1 (MQ=39)
                                                                                                                                           tGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTCGAACCTCCGACCCCTTCGTCCCGAACGAAGTGCGCTACCAGGCTGCGCCAATCACCGAATGCGGGGCGCATCTTACTGCGCAGATACGCCCTCGTCAATCCCt        <  2:305899/149‑1 (MQ=255)
                                                                                                                                             cgcGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTCGAACCTCCGACCCCTTCGTCCCGAACGAAGTGCGCTACCAGGCTGCGCCAATCACCGAATGCGGGGCGCATCTTACTGCGCAGATACGCCCTCGTCAATCCCTTa      <  2:600825/147‑1 (MQ=255)
                                                                                                                                               cGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTCGAACCTCCGACCCCTTCGTCCCGAACGAAGTGCGCTACCAGGCTGCGCCAATCACCGAATGCGGGGCGCATCTTACTGCGCAGATACGCCCTCGTCAATCCCTTAAt    <  1:417261/149‑1 (MQ=255)
                                                                                                                                               cGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTCGAACCTCCGACCCCTTCGTCCCGAACGAAGTGCGCTACCAGGCTGCGCCAATCACCGAATGCGGGGCGCATCTTACTGCGCAGATACGCCCTCGTCAATCCCTTAAt    <  2:653100/149‑1 (MQ=255)
                                                                                                                                               cGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTCGAACCTCCGACCCCTTCGTCCCGAACGAAGTGCGCTACCAGGCTGCGCCAATCACCGAATGCGGGGCGCATCTTACTGCGCAGATACGCCCTCGTCAATCCCTTAAt    <  2:592594/149‑1 (MQ=255)
                                                                                                                                                 ttGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTCGAACCTCCGACCCCTTCGTCCCGAACGAAGTGCGCTACCAGGCTGCGCCAATCACCGAATGCGGGGCGCATCTTACTGCGCAGATACGCCCTCGTCAATCCCTTAATAg  <  2:618090/149‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                    
TCGGTGATAGAGGATTACTCGCCACTTCGTGGCTCGCCCTGCGGGCCGTTGCTGGCGCAACGTTCTCTCGCTTCGCTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTCGAACCTCCGACCCCTTCGTCCCGAACGAAGTGCGCTACCAGGCTGCGCCAATCACCGAATGCGGGGCGCATCTTACTGCGCAGATACGCCCTCGTCAATCCCTTAATAG  >  NC_000913/3708451‑3708744

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: