Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,809,356 C→A 6.2% R273S (CGC→AGC)  waaA → 3‑deoxy‑D‑manno‑octulosonic‑acid transferase (KDO transferase)

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,809,3560CA 6.2% 280.8 / 5.6 115R273S (CGC→AGC) waaA3‑deoxy‑D‑manno‑octulosonic‑acid transferase (KDO transferase)
Reads supporting (aligned to +/- strand):  ref base C (55/51);  new base A (4/4);  total (59/56)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 116 total aligned reads displayed.

GCTGCGCCGCCAGTGGGCACCACACCGCCCGGTATGGATTGCCACCAGCACTCACGAAGGCGAAGAGAGTGTGGTGATCGCCGCACATCAGGCATTGTTACAGCAATTCCCGAATTTATTGCTCATCCTGGTACCCCGTCATCCGGAACGCTTCCCGGATGCGATTAACCTTGTCCGCCAGGCTGGACTAAGCTATATCACACGCTCTTCAGGGGAAGTCCCCTCCACCAGCACGCAGGTTGTGGTTGGCGATACGATGGGCGAGTTGATGTTACTGTATGGCATTGCCGATCTC  >  NC_000913/3809208‑3809502
                                                                                                                                                    |                                                                                                                                                  
    cgccgcCAGTGGGCACCACACCGCCCGGTATGGATTGCCACCAGCACTCACGCAGGCGAAGAGCGTGTGGTGATCGCCGCACATCAGGCATTGTTACAGCAATGCCCGAATTTATTGCTCATCCTGGTACCCCGTCATCCGGAACGCtt                                                                                                                                                >  2:578410/1‑149 (MQ=255)
     gccgccAGTGGGCACCACACCGCCGGGTATGGATTGCCACCAGCACTCACGACAGCGAAGAGAGTGTGGTGATCGCCGAACATCAGGAAATGTTACAGCAATTCCCGAATTTTTTGCTCATCCTGGTACCCAGTCATCCGGAACGCTTc                                                                                                                                               >  1:578979/1‑149 (MQ=255)
       cgccAGTGGGCACCACCCCGCCCGGTATGGATTGCCCCCAGCACTCACGAAGGCGAAGAGAGTGTGGTGATCGCCGCACATCAGGCATAGTTACCGCAATTCCCGCATTTATTGGTCATCCTGGTACACCGTCTTCCGGAACGCTTcgc                                                                                                                                             >  1:602082/1‑147 (MQ=255)
       cgccAGTGGGCACCACACCGCCCGGTAGGGATTGCCACCAGCACTCCCGAAGGCGAAGCGAGTGTGGTGATCGCCGCACATCAGGCATTGTTACAGCACTTCCCGAATGTATTGCTCCTCCTGGTACCCCGTCATCCGGAACGCTTccc                                                                                                                                             >  2:1013447/1‑149 (MQ=255)
          cAGTGGGCACCCCACCGCCCGGTATGGATTGCCACCAGCACTCACGAAGGCGAAGAGAGTGTGGTGATCGCCGCACATCAGGCATTGTGACAGCACTTCCCGAATTTATTGCTCATCCAGGTACCCCGTCATCCGGCTCGCTTCCCggt                                                                                                                                          >  1:31339/1‑148 (MQ=255)
             tGGGCACCACACCGCCCGGTATGGATTGCCACCAGCACTCACGAAGGCGAAGAGAGTGTGGTGATCGCCGCACATCAGGCATTGTTACAGCAATTCCCGAATTTATTGCTCATCCTGGTACCCCGTCATCCGGGAAGCGTTCCGGATga                                                                                                                                       <  1:21785/149‑2 (MQ=255)
             tGGGCACCACACCGCCCGGTATGGATTGCCACCAGCACTCACGAAGGCGAAGAGAGTGTGGTGATCGCCGCACATCAGGCATTGTTACAGCAATTCCCGAATTTATTGCTCATCCTGGTACCCCGTCATCCGGAACGCTTCCCGGATGc                                                                                                                                       <  1:283083/149‑1 (MQ=255)
             ggggCACCACACCGCCCGTTAAGGATTGCCACCAGCACTCACGAAGGCGAAGAGAGTGTGGTGATCGCCGCACATCAGGCATTGTTACAGCAATTCCCGAATTTATTGCTCATCCTGGTACCCCGTCATCCGGGAAGCGTTCCGGATga                                                                                                                                       <  2:622081/148‑2 (MQ=255)
               ggCACCCCACCGCCCGGTATGGATTGCCACCAGCACTCACGCAGGCGAAGAGAGTGTGGGGATCGCCGCACATCAGGCATTGTTACAGCAATTCCCGATTTTATTGCTCATCCTGGTACCCCGTCATCCGGAACGCTTCCCGGATGCGa                                                                                                                                     <  2:661619/149‑1 (MQ=255)
               ggCACCACACCGCCCGGTATGGATTGCCACCAGCTCTCACGAAGGCGATGAGATTGTGGTGATCGCCGCAGATCAGGCATTGTTACAGCAATTCCCGAATTTATTGCTCTTCCTGGTAGCCCGTCATCCGGAACGCTTCCCGGATGCGa                                                                                                                                     <  2:749656/149‑1 (MQ=255)
               ggCACCACACCGCCCGGTATGGATTGCCACCAGCACTAACGAAGGCGAAGAGAGTGTGGTGATCGCCGCACATCAGGCATTGTTACAGCAATTCCCGAATTTATTGCTCATCCTGGTACCCCGTCATCCGGAACGCTTCCCGGATGCGa                                                                                                                                     <  1:106326/149‑1 (MQ=255)
                 caccacACCGCCCGGTATGGATTGCCACCAGCACTCACGATGGCGAAGAGAGTGTGGTGATCGCCGCACAACAGGCATAGTTACAGCAATTCCCGAATTTATTGCTAATCCTGGTACCCCGTCAACAGGAAAGCTTCAAGGAAGCGaat                                                                                                                                   >  1:765250/1‑147 (MQ=255)
                 caccacACCGCCCGGTATGGATTGCCACCAGCACTCACGACGGCGAAGAGAGTGTGGTGATCGCCGCACATCAGGCATTGTTACAGCAATTCCCGAATTTATTGCTCATCCTGGTACCCCGTCATCCGGAACGCTTCCCGGATGCGAtt                                                                                                                                   >  1:731656/1‑149 (MQ=255)
                   caccacCGCCCGGTATGGCTTGCCACCAGCACTCACGAAGGCGAAGAGAGTGTGGTGATCGCCGCACATCAGGCATTGTTCCAGCAATTCACTAATTTATTGCTCATCCTGGTACCCCGTCATCCGGAACGCTTCCCGGATGCGATTaa                                                                                                                                 <  1:67650/146‑1 (MQ=255)
                       acCGCCCGGTATGGATTGCCACCAGCACTCACGAAGGCGAAGAGAGTGTGGTGATCGCCGCACATCAGGCATTGTTACAGCAATTCCCGAATTTATTGCTCATCCTGGTACCCCGTCATCCGGAACGCTTCCCGGATGCGATTAACCtt                                                                                                                             >  2:472830/1‑149 (MQ=255)
                        ccGCCCGGTATGGATTGCCACCAGCACTCACGACGGCGAAGAGAGTGTGGTGATCGCCGCACATCAGGCATTGTTACAGCAATTCCCGAATTTATTGCTCATCCTGGTACCCCGTCATCCGGAACGCTTCCCGGATGCGATTAACCTTg                                                                                                                            <  1:458377/149‑1 (MQ=255)
                         cGCCCGGTATGGATTGCCACCAGCACTCACGAAGGCGAAGAGATTGTGGTGATCTCCGCACATCAGGCATTGTTACAGCAATTCCCGAATTTATTGCTCATCCTGGTACCCCGTCATCCGGAACGCTTCCCGGATGAGATTTACCTTGt                                                                                                                           >  2:504503/1‑149 (MQ=255)
                         cGCCCGGTATGGATTGCCACCAGCACTCACGAAGGCGAAGAGAGTGTGGTGATCGCCGCACATCAGGCATTGTTACAGCAATTCCCGAATTTATTGCTCATCCTGGTACCCCGTCATCCGGAACGCTTCCCGGATGCGATTAACCTTGt                                                                                                                           >  1:981317/1‑149 (MQ=255)
                         cGCCCGGTATGGATTGCCACCAGCACTCACGAAGGCGAAGAGAGTGTGGTGATCGCCGCACATCAGGCATTGTTACAGCAATTCCCGAATTTATTGCTCATCCTGGTACCCCGTCATCCGGAACGCTTCCCGGATGCGATTAACCTTGt                                                                                                                           >  2:480936/1‑149 (MQ=255)
                          gCCCGGTATGGATTGCCACCAGCACTCACGAAGGCGAAGAGAGTGTGGTGATCGCCGCACATCAGGCATTGTTACAGCAATTCCCGAATTTATTGCTCATCCTGGTACCCCGTCATCCGGAACGCTTCCCGGATGCGATTAACCTTGTc                                                                                                                          <  2:215994/149‑1 (MQ=255)
                           cccGGTATGGATTGCCACCAGCACTCACGAAGGCGAAGAGAGTGTGGTGCTCGCCGCGCATCAGGCATTGTTACAGCAATTCCCGAATTTATTGCTCATCCTGGTACCCCGTCATCCGGAACGCTTCCCGGATGCGATTAACCTTCTcc                                                                                                                         >  1:135813/1‑149 (MQ=255)
                              ggTATGGATTGCCACCAGCACTCACGAAGGCGAAGAGAGTGTGGTGATCGCCGCACATCAGGCATTGTTACAGCAATTCCCGAATTTATTGCTCATCCTGGTACCCCGTCATCCGGAACGCTTCCCGGATGCGATTAACCTTGTCCGcc                                                                                                                      <  1:187282/149‑1 (MQ=255)
                               gctaggAATGACACCATCACTCACGATGGCGTAGAGAGTTTCTGGATCGCCGCACAGCAGCCATTGTTACAGCAATTCCCGAATTTCTTGCTCCTCCTGGTACCCCGGCATCGGGAACGCTTCCCGGATGCGATTAACCTTGTCCGCCa                                                                                                                     <  2:748281/145‑1 (MQ=255)
                                tATGGATTGCCACCAGCACTCACGAAGTCGAAGAGAGTGTGGTGATCGCCGCACATCAGGCATTGTTACAGCAATTCCCGAATTTATTGCTCATCCTGGTACCCCGTCATCCGGAACGCTTCCCGGATGCGATTAACCTTGTCCGCCAg                                                                                                                    <  2:697769/149‑1 (MQ=255)
                                   ggATTGCCACCAGCACTCACGAAGGCGAAGAGAGTGTGGTGATCGCCGCACAGCAGGCATTGTTACAGCAATTCCCGAATTTATTGCTAATCCTCAAACCCCGTCAACCATAACGATTCACGAATTCTATTAACCTTGACAGCCacaaa                                                                                                                 >  2:680107/1‑145 (MQ=255)
                                    gATTGCCACCAGCACTCACGAAGGCGAAGAGAGTGTGGTGATCGCCGCACATCAGGCATTGTTACAGCAATTCCCGAATTTATTGCTCATCCTGGTACCCCGTCANCCGGAACNCTTCCCGGATGCGATTAACCTTGTCCGCCAGGCTg                                                                                                                <  1:356532/149‑1 (MQ=255)
                                    gATTGCCACCAGCACTCACGAAGGCGAAGAGAGTGTGGTGATCGCCGCACATCAGGCATTGTTACAGCAAGTCCCGAATTTATTGCTCATCCTGGTACCCCGTCATCCGGAACGCTTCCGGGATGCGATTAACCTTGTCCGCCAGGCTg                                                                                                                >  2:833585/1‑149 (MQ=255)
                                         ccaccaGCACTCACGAAGGCGAAGAGAGTGTGGTGATCGCCGCACATCAGGCATTGTTACAGCAATTCCCGAATTTATTGCTCATCCTGGTACCCCGTCATCCGGAACGCTTCCCGGATGCGATTAACCTTGTCCGCCAGGCTGGACTa                                                                                                           >  1:494328/1‑149 (MQ=255)
                                         ccaccaGCACTCACGAAGGCGAAGAGAGTGTGGTGATCGCCGCACATCAGGCATTGTTACAGCAATTCCCGAATTTATTGCTCATCCTGGTACCCCGTCATCCGGAACGCATCCCGGATGCGATTAACCTTGTCCGCCAGGCTGGACTa                                                                                                           >  2:949395/1‑149 (MQ=255)
                                           accaGCACTCACGAAGGCGAAGAGAGTGTGGTGATCGCCGCACATCAGGCATTGTTACACCAATTCCCGAATTTATTGCTCATCCTGGTACCCCGTCATCCGGAACGCTTCCCGGATGCGATTAACCTTGTCCGCCAGGCTGGACTAAg                                                                                                         <  1:437796/149‑1 (MQ=255)
                                           accaGCACTACCGAAGGCGAAGAGAGTGTGGTGATCGCCGCACATCAGGCATTGTTACAGCAATTCCCGCATTTATTGCTCATCCTGGTACCCCGTCATCCGGAACGCTTCCCGGATGCGATTATCCTTGTCCGCCAGGCTGGACTAAg                                                                                                         >  2:777319/1‑149 (MQ=255)
                                            ccaGCACTCACGAAGGCGAAGAGAGTGTGGTGATCGCCGCACATCAGGCATTGTTACAGCAATTCCCGAATTTATTGCTCATCCTGGTACCCCGTCATCCGGAACGCTTCCCGGATGCGATTAACCTTGTCCGCCAGGCTGGACTAAGc                                                                                                        >  1:153406/1‑149 (MQ=255)
                                            ccaGCACTCACGAAGGCGAAGAGAGTGTGGTGATCGCCGCACATCAGGCATTGTTACAGCAATTCCCGAATTTATTGCTCATCCTGGTACCCCGTCATCCGGAACGCTTCCCGGATGCGATTAACCTTGTCCGCCAGGCTGGACTAAGc                                                                                                        <  2:269217/149‑1 (MQ=255)
                                            ccaGCACTCACGAAGGCGAAGAGAGTGTGGGTATCGCCGCACATCAGGCATTGTTACAGCAATTCCCGAATTTATTGCTCATCCTGGTACCCCGTCATCCGGAACGCTTCCCGGATGCGATTAACCTTGTCCGCCAGGCTGGACTAAGc                                                                                                        <  1:728160/149‑1 (MQ=255)
                                             caGCACTCACGAAGGCGAAGAGAGTGTGGTGATCGCCGCACATCAGGCATTGTTACAGCAATTCCCGAATTTATTGCTCATCCTGGTACCCCGTCATCCGGAACGCTTCCCGGATGCGATTAACCTTGTCCGCCAGGCTGGACTAAGCt                                                                                                       <  1:711708/149‑1 (MQ=255)
                                             caGCACTCACGAAGGCGAAGAGAGTGTGGTGATCGCCGCACATCAGGCATTGTTACAGCAATTCCCGAATTTATTGCTCATCCTGGTACCCCGTCATCCGGAACGCTTCCCGGATGCGATTAACCTTGTCCGCCAGGCTGGACTAAGCt                                                                                                       >  1:413770/1‑149 (MQ=255)
                                             caGCACTCACGAAGGCGAAGAGAGTGTGGTGATCGCCGCACATCAGGCATTGTTACAGCAATTCCCGAATTTATTGCTCATCCTGGTACCCCGTCATCCGGAACGCTTCCCGGATGCGATTAACCTTGTCCGCCAGGCTGGACTAAGCt                                                                                                       <  1:18265/149‑1 (MQ=255)
                                               gCACTCACGAAGGCGAATAGAGTGTGGTGATGGCCGCACATCAGGCATTGTTACAGCAATTCCCGAATTTATTGCTCATCCTGGTACCCCGTCATCCGGACCGCTTCCCGTATGCGTTTACCCTTGTCCGCCAGGCTGGACTAAGCtat                                                                                                     <  2:868024/149‑1 (MQ=255)
                                                  cTCACGAAGGCGAAGAGAGTGTGGTGATCGCCGCACATCAGGCATTGTTACAGCAATTCCCGAATTTATTGCTCATCCTTGTACCCCGTCATCCGGAACGCTTCCCGGATGCGATTAACCTTGTCCGCCAGGCTGGACTAAGCTATATc                                                                                                  <  1:473106/149‑1 (MQ=255)
                                                   tCACGAAGGCGAAGAGAGTGTGGTGATCGCCGCACATCAGGCATTGTTACAGCAATTCCCGAATTTATTGCTCATCCTGGTACCCCGTCATCCGGAACGCTTCCCGGATGCGATTAACCTTGTCCGCCAGGCTGGACTAAGCTATATca                                                                                                 >  2:224517/1‑149 (MQ=255)
                                                    cACGAAGGCGAAGAGAGTGTGGTGATCGCCGCACATCAGGCATTGTTACAGCAATTCCCGAATTTATTGCTCATCCTGGTACCCCGTCATCCGGAACGCTTCCCGGATGCGATTAACCTTGTCCGCCAGGCTGGACTAAGCTATATcac                                                                                                <  1:886672/149‑1 (MQ=255)
                                                     aCGAAGGCGAAGAGAGTGTGGTGATCGCCGCACATCAGGCATTGTTACAGCAATTCCCGAATTTATTGCTCATCCTGGTACCCCGTCATCCGGAACGCTTCCCGGATGCGATTAACCTTGTCCGCCAGGCTGGACTAAGCTATATcacc                                                                                               >  2:798993/1‑148 (MQ=255)
                                                     aCGAAGGCGAAGAGAGTGTGGTGATCGCCGCACATCAGGCATTGTTACAGCAATTCCCGAATTTATTGCTCATCCTGGTACCCCGTCATCCGGAACGCTTCCCGGATGCGATTAACCTTGTCCGCCAGGCTGGACTAAGCTATATcaca                                                                                               <  2:392619/149‑1 (MQ=255)
                                                       gAAGGCGAAGAGAGTGTGGTGATCGCCGCACATCAGGCATTGTTACAGCAATTCCCGAATTTATTGCTCATCCTGGTACCCCGTCATCCGGACCTCGTCCCGGATGCGATTAACCTTGTCCGCCAGGCTGGACTACGCTATATCACACg                                                                                             >  1:745368/1‑149 (MQ=255)
                                                       gAAGGCGAAGAGAGTGTGGTGATCGCCGCACATCAGGCATTGTTACAGCAATTCCCGAATTTATTGCTCATCCTGGTACACCGTCATCCGGAACGCTTCCCGGATGCGATTAACCTTGTCCGCCAGGCTGGACTAAGCTATATCACACg                                                                                             >  1:221137/1‑149 (MQ=255)
                                                       gAAGGCGAAGAGAGTGGGGTGATCGCCGCACATCAGGCATGGTTACAGCAATTCCCGAATTTATTGCTCATCCTGGTACCCCGTCATCCGGAACGCTTCCCGGATGCGATTAACCTTGTCCGCCAGGCTGGACTAAGCTATATCACACg                                                                                             <  2:377037/149‑1 (MQ=255)
                                                            cGAAGAGAGTGTGGTGATCGCCGCACATCAGGCATTGTTACAGCAATTCCCGAATTTATTGCTCATCCTGGTACCCCGTCATCCGGAACGCTTCCCGGATGCGATTAACCTTGTCCGCCAGGCTGGACTAAGCTATATCACACGCTCtt                                                                                        <  2:307909/149‑1 (MQ=255)
                                                             gAAGAGAGTGTGGTGATCGCCGCACATCAGGCATTGTTACAGCAATTCCCGAATTTATTGCTCATCCTGTTACCCCGTCATCCGGAACGCTGCCCGGCTGCGATTTACCTTGTCCGCCAGGCTGGACTTAGCTATATCACACGCTCTTc                                                                                       >  2:314194/1‑149 (MQ=255)
                                                             gAAGAGAGTGTGGTGATCGCCGCACATCAGGCATTGTTACAGCAATTCCCGAATTTATTGCTCATCCTGGTACCCCGTCATCCGGAACGCTTCCCGGATGCGATTAACCTTGTCCGCCAGGCTGGCCTAAGCTATATCACACGCTCTTc                                                                                       >  1:182393/1‑149 (MQ=255)
                                                             gAAGAGAGTGTGGTGATCGCCGCACATCAGGCATTGTTACAGCAATTCCCGAATTTATTGCTCATCCTGGTACCCCGTCATCCGGAACGCTTCCCGGATGCGATTAACCTTGTCCGCCAGGCTGGACTAAGCTATATCACACGCTCTTc                                                                                       >  1:809678/1‑149 (MQ=255)
                                                                 tgagTGTGGTGATCGCCGCACATCAGGCATTGTTACAGCACTTCCCGAATTTATTGCTCAACCTGGTACCCCGTCATCCGGAACGCTTTCCGGATGCGATTAACCTTGTCCGCCAGGCTGGACTAAGCTATATCACACGCTATTCAggg                                                                                   <  2:283156/148‑1 (MQ=255)
                                                                 agagTGTGGTGATCGCCGCACATCAGGCATTGTTACAGCAATTCCCGAATTTATTGCTCATCCTGGTACCCCGTCATCCGGAACGCTTCCCGGATGCGATTAACCTTGTCCGCCAGGCTGGACTAAGCTATATCACACGCTCTTCAggg                                                                                   <  2:392717/149‑1 (MQ=255)
                                                                  gtgtgtGGTGATCGCCGCAGATCAGGCATTGTTACAGCAATTCCCGAATTTATTGCTCATCCTGGTACCCCGTCATCCGGAACGCTTCCGGGATGCGATTAACCTTGTCCGCCAGGCTGGACTAAGCTATATCACACGCTCTTCAgggg                                                                                  <  2:983850/147‑1 (MQ=255)
                                                                      gtggtgATCGCCGCACATCAGGCATTGTTACAGCAATTCCCGAATTTATTGCTCATCCTGGTACCCCGTCATCCGGAACGCTTCCCGGATGCGATTAACCTTGTCCGCCAGGCTGGACTAAGCTATATCACACGCTCTTCAGGGGAAGt                                                                              <  1:543844/149‑1 (MQ=255)
                                                                       tggtgATCGCCGCACATCAGGCATTGTTACAGCAATTCCCGAATTTATTGCTCATCCTGGTCCCCCGTCCTCCGGAACGCTTCCCGGATGCGATTACCCTTGTCCGCCAGGCTGGACTAAGCTATATCACACGCTCTTCAGGGGAAGTc                                                                             >  1:893890/1‑149 (MQ=255)
                                                                       tggtgATCGCCGCACATCAGGCATTGTTACAGCAATTCCCGAATTTATTGCTCATCCTGGTACCCCGTCATCCGGATCGCTTCCCGGATGCGATTAACCTTGTCCTCCAGGCTGGACTAAGCTATATCACACGCTCTACAGGGGAAGTc                                                                             >  1:313575/1‑149 (MQ=255)
                                                                         gtgATCGCCGCACATCAGGCATTGTTACAGCAATTCCCGAATTTATTGCTCATCCTGGTACCCCGTCATCCGGAACGCTTCCCGGATGCGATTAACCTTGTCCGCCAGGCTGGACTAAGCTATATCACACGCTCTTCAGGGGAAGTccc                                                                           <  2:12606/149‑1 (MQ=255)
                                                                           gATCGCCGCACATCAGGCATTGTTACAGCAATTCCCGAATTTATTGCTCATCCTGGTACCCCGTCATCCGGAACGCTTCCCGGATGCGATTAACCTTGTCCGCCAGGCTGGACTAAGCTATATCACACGCTCTTCAGGGGAAGTccccg                                                                         >  2:884894/1‑148 (MQ=255)
                                                                            aTCGCCGCACATCAGGCATTGTTACAGCAATTCCCGAATTTATTGCTCATCCTGGTACCCCGTCATCCGGAACGCTACCCGGATGCGATTAACCTTGTCCGCCAGGCTGGACTAAGCTATATCACACGCTCTTCAGGGGAAGTCCCCTc                                                                        <  1:560072/149‑1 (MQ=255)
                                                                            aTCGCCGCACATCAGGCATTGTTACAGCAATTCCCGAATTTATTGCTCATCCTGGTACCCCGTCATCCGGAACGCTACCCGGATGCGATTAACCTTGTCCGCCAGGCTGGACTAAGCTATATCACACGCTCTTCAGGGGAAGTCCCCTc                                                                        <  1:154141/149‑1 (MQ=255)
                                                                               gccgcACATCAGGCATTGTTACAGCAATTCCCGAATTTATTGCTCATCCTGGTACACCGTCATCCGGAACGCTTCCCGGATGCGATTAACCTTGTCCGCCAGGCTGGACTAAGCTATATCACACGCTCTTCAGGGGAAGTCCCCCccac                                                                     <  1:504503/149‑1 (MQ=255)
                                                                                 cgcACATCAGGCATTGTTACAGCAATTCCCGAATTTATTGCTCCTCCTGGTACCCCGTCATCCGGAACGCTTCCCGGATGCGATTAACCTTGTCCGCCAGGCTGGACTAAGCTATATCACACGCTCTTCAGGGGAAGTCCCCTccacca                                                                   <  2:503723/149‑1 (MQ=255)
                                                                                   cacaTCAGGCATTGTTACAGCAATTCCCGAATTTATTGCTCATCCTGGTACCCCTTCATCCGGAACGCTTCCCGGATGCGATTAACCTTGGCCGCCAGGCTGGACTAAGCTATATCACACGCTCTTCAGGGGAAGTCCCCTCCACCAGc                                                                 <  2:731656/149‑1 (MQ=255)
                                                                                      aTCAGGCATTGTTACAGCAATTCCCGAATTTATTGCTCATCCTGGTACCCCGTCATCCGGAACGCTTCCCGGATGCGATTACCCTTGTCCGCCAGGCTGGACTAAGCTATATCACACGCTCTTCAGGGGAAGTCCCCTCCACCAGCACg                                                              >  2:419025/1‑149 (MQ=255)
                                                                                      aTCAGGCATTGTTACAGCAATTCCCGAATTTATTGCTCATCCTGGTACCCCGTCATCCGGAACGCTTCCCGGATGCGATTAACCTTGTCCGCCAGGCTGGCCTAAGCTATATCACGCGCTCTTCAGGGGAAGTCCCCTCCACCAGCACg                                                              >  1:945343/1‑149 (MQ=255)
                                                                                       tCAGGCATTGTTACAGCAATTCCCGAATTTATTGTTCATCCTGGTACCCCGTCATCCGGAACGCTTCCCGGATGCGATTAACCTTGTCCGCCAGGCTGGACTAAGCTATATCACACGCTCTTCAGGGGAAGTCCCCTCCACCAGCACGc                                                             >  1:890920/1‑149 (MQ=255)
                                                                                       tCAGGCATTGTTACAGCAATTCCCGAATTTATTGCTCATCCTGGAACCCCGTCATCCGGAACGCTTACCGGATGCGATTAACCTTGTCCGCCAGGCTGGACTAAGGTTTATCACACGCTCTTCAGGGGAAGTCCCCTCCACCAGCACGc                                                             >  2:616392/1‑149 (MQ=255)
                                                                                        cAGGCATTGTTACAGCAATACCCGAATTTATAGCTCATCCTGGTACGCCGTCATCCGTAACGCTTCCCGGATGCGATTAACCTTGTCCGCCAGGCTGGACTAAGCTATATCACACGCTCTTCAGGGGAAGTCCCCTCCACCAGCACGct                                                            <  1:52650/149‑2 (MQ=255)
                                                                                          ggCATTGTTACAGCAATTCCCGAATTTATTGCTCATCCTGTTACCCCGTCATCCGGAACGCTTCCCGGATGCGATTAACCTTGTCCGCCAGGCTGGACTAAGCTATATCAAACGCTCTTCACGGGATGTCCCCTCCACACGCACGCAgg                                                          >  2:472848/1‑149 (MQ=255)
                                                                                                gTTACAGCAATTCCCGAATTTATTGCTCATCCTGGTACCCCGTCATCCGGAACGCTTCCCGGATGCGATTAACCTTGTCCGCCAGGCTGGACTAAGCTATATCACCCGCTCTACAGGGGAAGTCCCCTCCACCAGCACGCAGGTTGTgg                                                    >  2:244486/1‑149 (MQ=255)
                                                                                                 ttACAGCAATTCCCGAATTTATTGCTCATCCTGGTACCCCGTCATCCGGAACGCTTCCCGGATGCGATTACCCTTGTCCGCCAGGCTGGACTAAGCTATATCACACGCTCTTCAGGGGAAGTCCCCTCCACCAGCACGCAGGTTGTGGt                                                   >  2:161009/1‑149 (MQ=255)
                                                                                                  tACAGCAATTCCCGAATTTATTGCTCATCCTGGTACCCCGTCATCCGGAACGCTTCCCGGATGCGATTAACCTTGTCCGCCAGGCTGGACTAAGCTATATCACACGCTCTTCAGGGGAAGTCCCCTCCACCAGCACGCAGGTTGTGGtt                                                  <  1:480936/149‑1 (MQ=255)
                                                                                                  tACAGCAATTCCCGAATTTATTGCTCATCCTCGTACCCCGTCATCCGGAACGCTTCCCGGATGCGATTAACCTTGTCCGCCAGGCTGGACTAAGCTATATCACACGCTCTTCAGGGGAAGTCCCCTCCACCAGCACGCAGGGTGTGGtt                                                  <  2:66417/149‑1 (MQ=255)
                                                                                                   aCAGCAATTCCCGAATTTATTGCTCATCCTGGTACCCCGTCATCCGGAACGCTTCCCGGATGCGATTAACCTTGTCCGCCAGGCTGGACTAAGCTATATCACACGCTCTTCAGGGGAAGTCCCCTCCACCAGCACGCAGGTTGTGGTTg                                                 >  2:813510/1‑149 (MQ=255)
                                                                                                   aCAGCAATTCCCGAATATATTGCTCATCCTGGTACCCCGGCATCCGGAACGCTTCCCGGATGCGATTAACCTTGTCCGCCAGGCTGGACTAAGCTATATCACACGCTCTTCAGGGGAAGTCCCCTCCACCAGCACGCAGGTTGTGGTTg                                                 <  2:534498/149‑1 (MQ=255)
                                                                                                     cgCAAATCCCGAATTTATTGCTCATCCTGGTACCCCGTCATCCGGAACGCGTCCCGGATGCGATTAACCTGGTCCGCCAGGCTGGACTACGCTATATCCCACGCTCTTCAGGGGCAGTCCCCTCCCCCCGCCCGCAGGTTGTGGTTGGc                                               <  2:970587/148‑1 (MQ=255)
                                                                                                     aGCAATTCCCGAATTTATTGCTCATCCTGGTACCCCGTCATCCGGAACGCTTCCCGGATGCGATTAACCTTGTCCGCCAGGCTGGACTAAGCTATATCACCCGCTCTTCAGGGGAAGTCCCCTCCACCAGCACGCAGGTTGTGGTTGGc                                               <  2:118800/149‑1 (MQ=255)
                                                                                                     aGCAATTCCCGAATTTATTGCTCATCCTCGTACCCCGTCATCCGGAACCCTTCCCGGAACAGAATAACCTTGTCCGACAGGCTGGACTAAGCAAAATCACAAGCTCTTCTGCGGAAGTCCCCTCCACCAGCACGCAGGTTGTGGATGGc                                               >  1:589444/1‑149 (MQ=255)
                                                                                                       cAATTCCCGAATTTATTGCTCATCCTGGTACCCCGTCATCCGGAACGCTTCCCGGATGCGATTAACCTTGTCCGCCAGGCTGGACTAAGCTATATCACACGCTCTTCAGGGGAAGTCGCCTCCACCAGCACGGAGGTTGTGGTTGGCGa                                             >  1:476747/1‑149 (MQ=255)
                                                                                                             ccGAATTTATTGCTCATCCTGGTACCCCGTCATCCGGAACGCTTCCCGGATGCGATTAACCTTGTCCGCCAGGCTGGACTAAGCTATATCACACGCTCTTCAGGGGAAGTCCCCTCCCACAGCACGCAGGTTGTGGTGGGCGATACGAt                                       >  2:382512/1‑149 (MQ=255)
                                                                                                             ccGAATTTATTGCTCATCCTGGTACCCCGTCATCCGGAACGCTTCCCGGATGCGATTAACCTTGTCCGCCAGGCTGGACTAAGCTATATCACACGCTCTTCAGGGGAAGTCCCCTCCACCAGCACGCAGGTGGTGGTTGGCGAGACGAt                                       >  2:636090/1‑149 (MQ=255)
                                                                                                             ccGAATTTATTGCTCATCCTGGTACCCCGTCATCCGGAACGCTTCCCGGATGCGATTAACCTTCTCCGCAAGGCTGGACTAAGCTAGATCACACGCTCTTCAGGGGAAGACCCCTCCAGCAGCACGCAGGGGGTGGTAGGCGAGACGAt                                       >  1:669703/1‑149 (MQ=255)
                                                                                                              cGAATTTATTGCTCATCCTGGTACCCCGTCATCCGGAACGCTTCCCGGATGCGATTAACCTTGTCCGCCAGGCTGGACTAAGCTATATCACACGCTCTTCAGGGGAAGTCCCCTCCACCAGCACGCAGGTTGTGGTTGGCGATACGATg                                      >  1:838818/1‑149 (MQ=255)
                                                                                                               gAATTTATTGCTCATCCTGGTACCCCGTCATCCGGAACGCTACCCGGATGCGATTAACCTTGTCCGCCAGGCTGGACTAAGCTATATCACACGCTCTTCAGGGGAAGTCCCCTCCACCAGCACGCAGGTTGGGGGTGGCGATAGGATgg                                     >  2:464770/1‑149 (MQ=255)
                                                                                                                aaTTAAATGCGCATCCTGGTACCCCGTCATCCGGAACGCTTCCCGGATGCGATTAACCTTGTCCGCCAGGCTGGACTAAGCTATATCACACGCTCTTCAGGGGAAGTCCCCTCCACCAGCACGCAGGTTGTGGTTGGCGTTACGATggg                                    <  1:1033250/149‑1 (MQ=255)
                                                                                                                        gCTGATCCTGGTACCCCGTCCTCCGGAACGCTTCCCGGATGCGATTAACCTTGACCGCCAGGCTGGACTAAGCTATATCACACGCTCTTCAGGTGAAGTCTCATCCACCAGCTCGGAGGTTGTGGTTGGCGATACGATGTGCGAGTTGa                            <  2:765250/149‑1 (MQ=255)
                                                                                                                        gCTCATCCTGGTACCCCGTCATCGGGAACGCTTCCCGGATGCGATTAACCTTGTCCGCCAGGCTGGACTAAGCTATATCACACGCTCTTCAGGGGAAGTCCCCTCCACCAGCACGCAGGGTGTGGTTGGCGATACGATGGGCGAGTTGa                            <  1:814382/149‑1 (MQ=255)
                                                                                                                        gCTCATCCTGGTACCCCGTCATCCGGAACGCTTCCCGGATGCGATTAACCTTGTCCGCCAGGCTGGACTAAGCTATATCACACGCTCTTCAGGGGAAGTCCCCTCCACCAGCACGCAGGTTGTGGTTGGCGATACGATGGGCGAGTTGa                            <  1:161009/149‑1 (MQ=255)
                                                                                                                        gCTCATCCTGGTACACCGTCATACGGAACGCTTCCCGGATGCGATTACACTTGTCCTCCAGGCTGGACTAAGCGATATAACACGCACTTCAGAGGAAGTCCCCTCAACCAGCACGCAGTGTGTGGTTGGCGATCCGATGGGCTAGTGGa                            >  2:927021/1‑149 (MQ=255)
                                                                                                                         cTCATCCTGGTACCCCGTCATCCGGCACGCTTCCCGGATGCGATTAACCTTGTCCGCCAGGCTGGACTATGCTATATCACACGCTCTTCAGGGGAAGTCCCCTGCACCATCACGCAGGTTGTGGTGGGCGATACGATGTGAGAGTTGAt                           >  1:583607/1‑149 (MQ=255)
                                                                                                                         cTCATCCTGGTACACCGTCATCCGGAACGCTTCCCGGATGCGATTAACCTTGTCCGCCAGGCTGGACTAAGCTATATCACACGCTCTTCAGGGGAAGTCCCCTCCACCAGCACGCAGGATGAGGTTGGCGATACGATGGGCGAGTTGAt                           >  2:367828/1‑149 (MQ=255)
                                                                                                                           cATCCTGGTACCCCGTCATCCGGAACGCTTCCCGGATGCGATTAACCTTGTCCGCCAGGCTGGACTAAGCTATATCACACGCTCTTCAGGGGAAGTCCCCTCCACCAGCACGCAGGTTGTGGTTGGCGATACGATGGGCGAGTTGATGt                         <  1:126565/149‑1 (MQ=255)
                                                                                                                             tgcTGGGACCCCCGCCTCCGGAACGGTTGCCGGTTGGGGTTAACCTTTTGCGCCATGCTGGTCTTAGCTATATCACACGCTCTTCAGGGGAAGTCCCCTCCACCAGCACGCAGGTTGTGGTTGGCGATACGATGGGCGAGTTGATGTTa                       <  2:981317/147‑1 (MQ=255)
                                                                                                                                     ccccGTCATCCGGAACGCTTCCCGGATGCGATTAACCTTGACCGCCAGGCTGGACTAAGCTATATCACACGCTCTTCAGGGGAAGTCCCCTCCACCTGCACGCAGCTTGTGGTTGGCGATACGATGGGCGCCTTGATGTTACTGTATgg               >  1:844820/1‑149 (MQ=255)
                                                                                                                                       ccGTCATCCGGAACGCTTGCCGGATGCGATTAACCTTGTCCGCCAGGCTGGACTAAGCTATATCACACGCTCTTCAGGGGAAGTCCCCTCCACCAGCACGCAGGTTGTGGTTGGCGATTCGATGGGCGAGTTGATGTTACTGTATGGCa             >  1:717165/1‑149 (MQ=255)
                                                                                                                                        tcgcATCCGGGAAGCGTTCCGGATGCGATTAACCTTGTCCGCCAGGCTGGACTAAGCTATATCACACGCTCTTCAGGGGAAGTCCCCTCCACCAGCACGCAGGTTGTGGTTGGCGATACGATGGGCGAGTTGATGTTACTGTATGGCAt            >  1:868466/4‑149 (MQ=255)
                                                                                                                                         cgcATCCGGGAAGCGTTCCGGATGCGATTAACCTTGTCCGCCAGGCTGGACTAAGCTATATCACACGCTCTTCAGGGGAAGTCCCCTCCACCAGCACGCAGGTTGTGGTTGGCGATACGATGGGCGAGTTGATGTACTGTATGGCATTg          >  2:777444/3‑149 (MQ=255)
                                                                                                                                            aTCCGGGAAGCGTTCCGGATGCGATTAACCTTGTCCGCCAGGCTGGACTAAGCTATATCACACGCTCTTCAGGGGAAGTCCCCTCCACCAGCACGCAGGTTGTGGTTGGCGATACGATGGGCGAGTTGATGTTACTGTATGGCATTGcc        >  2:262695/1‑149 (MQ=255)
                                                                                                                                             tCCGGAACGCTTCCCGGATGCGATTAACCTTGTCCGCCAGGCTGGACTAAGCTATATCCCACGCTCTTCAGGGGAAGTCCCCTCCACCAGCACGCAGGTTGTGGTTGGCGATACGATGGGCGAGTTGATGTTACTGTATGGCATTGCCg       >  1:202809/1‑149 (MQ=255)
                                                                                                                                                  aaCGCTTCCCGGATGCGATTAACCTTGTCCGCCAGGCTGGACTAAGCTATATCACACGCTCTTCAGGGGAAGTACCCTCCACCAGCACGCAGGTTGTGGTTGGCGATACGATGGGCGAGTTGATGTTACTGTATGGCATTGCCGAtctc  <  2:413770/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                  
GCTGCGCCGCCAGTGGGCACCACACCGCCCGGTATGGATTGCCACCAGCACTCACGAAGGCGAAGAGAGTGTGGTGATCGCCGCACATCAGGCATTGTTACAGCAATTCCCGAATTTATTGCTCATCCTGGTACCCCGTCATCCGGAACGCTTCCCGGATGCGATTAACCTTGTCCGCCAGGCTGGACTAAGCTATATCACACGCTCTTCAGGGGAAGTCCCCTCCACCAGCACGCAGGTTGTGGTTGGCGATACGATGGGCGAGTTGATGTTACTGTATGGCATTGCCGATCTC  >  NC_000913/3809208‑3809502

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: