Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,809,359 T→G 6.9% F274V (TTC→GTC) ‡ waaA → 3‑deoxy‑D‑manno‑octulosonic‑acid transferase (KDO transferase)

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,809,3590TG 6.9% 257.8 / 5.2 113F274V (TTC→GTC) ‡waaA3‑deoxy‑D‑manno‑octulosonic‑acid transferase (KDO transferase)
Reads supporting (aligned to +/- strand):  ref base T (52/51);  new base G (5/4);  total (58/55)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.58e-01

Only 100 of 114 total aligned reads displayed.

CGCCGCCAGTGGGCACCACACCGCCCGGTATGGATTGCCACCAGCACTCACGAAGGCGAAGAGAGTGTGGTGATCGCCGCACATCAGGCATTGTTACAGCAATTCCCGAATTTATTGCTCATCCTGGTACCCCGTCATCCGGAACGCTTCCCGGATGCGATTAACCTTGTCCGCCAGGCTGGACTAAGCTATATCACACGCTCTTCAGGGGAAGTCCCCTCCACCAGCACGCAGGTTGTGGTTGGCGATACGATGGGCGAGTTGATGTTACTGTATGGCATTGCCGATCTC  >  NC_000913/3809212‑3809502
                                                                                                                                                   |                                                                                                                                               
cgccgcCAGTGGGCACCACACCGCCCGGTATGGATTGCCACCAGCACTCACGCAGGCGAAGAGCGTGTGGTGATCGCCGCACATCAGGCATTGTTACAGCAATGCCCGAATTTATTGCTCATCCTGGTACCCCGTCATCCGGAACGCtt                                                                                                                                                >  2:578410/1‑149 (MQ=255)
   cgccAGTGGGCACCACCCCGCCCGGTATGGATTGCCCCCAGCACTCACGAAGGCGAAGAGAGTGTGGTGATCGCCGCACATCAGGCATAGTTACCGCAATTCCCGCATTTATTGGTCATCCTGGTACACCGTCTTCCGGAACGCTTcgc                                                                                                                                             >  1:602082/1‑147 (MQ=255)
   cgccAGTGGGCACCACACCGCCCGGTATGGATTGCCACCAGCACTCACGAAGGCGAAGAGAGTGGGGTGCTCGCCGGGCATCAGGCATTGTTACAGCAATTCCCGAGTTTATTGCTCATCCTGGTACCCCGTCATCCGGAACGCTTccc                                                                                                                                             >  1:234619/1‑149 (MQ=255)
         tGGGCACCACACCGCCCGGTATGGATTGCCACCAGCACTCACGAAGGCGAAGAGAGTGTGGTGATCGCCGCACATCAGGCATTGTTACAGCAATTCCCGAATTTATTGCTCATCCTGGTACCCCGTCATCCGGGAAGCGTTCCGGATga                                                                                                                                       <  1:21785/149‑2 (MQ=255)
         tGGGCACCACACCGCCCGGTATGGATTGCCACCAGCACTCACGAAGGCGAAGAGAGTGTGGTGATCGCCGCACATCAGGCATTGTTACAGCAATTCCCGAATTTATTGCTCATCCTGGTACCCCGTCATCCGGAACGCTTCCCGGATGc                                                                                                                                       <  1:283083/149‑1 (MQ=255)
         ggggCACCACACCGCCCGTTAAGGATTGCCACCAGCACTCACGAAGGCGAAGAGAGTGTGGTGATCGCCGCACATCAGGCATTGTTACAGCAATTCCCGAATTTATTGCTCATCCTGGTACCCCGTCATCCGGGAAGCGTTCCGGATga                                                                                                                                       <  2:622081/148‑2 (MQ=255)
           ggCACCCCACCGCCCGGTATGGATTGCCACCAGCACTCACGCAGGCGAAGAGAGTGTGGGGATCGCCGCACATCAGGCATTGTTACAGCAATTCCCGATTTTATTGCTCATCCTGGTACCCCGTCATCCGGAACGCTTCCCGGATGCGa                                                                                                                                     <  2:661619/149‑1 (MQ=255)
           ggCACCACACCGCCCGGTATGGATTGCCACCAGCACTAACGAAGGCGAAGAGAGTGTGGTGATCGCCGCACATCAGGCATTGTTACAGCAATTCCCGAATTTATTGCTCATCCTGGTACCCCGTCATCCGGAACGCTTCCCGGATGCGa                                                                                                                                     <  1:106326/149‑1 (MQ=255)
            gCACCACACCGCCCGGTATGGATTGCCACCAGCACTCACGACGGCGAAGAGAGTGTGGTGATCGCCGCACATCAGGCATTGTTACAGCAATTCCCGAATTTATTGCTCATCCTGGTACCCCGTCATCCGGGAAGCGTTCCGGATgacgg                                                                                                                                    <  1:16125/149‑5 (MQ=255)
             caccacACCGCCCGGTATGGATTGCCACCAGCACTCACGATGGCGAAGAGAGTGTGGTGATCGCCGCACAACAGGCATAGTTACAGCAATTCCCGAATTTATTGCTAATCCTGGTACCCCGTCAACAGGAAAGCTTCAAGGAAGCGaat                                                                                                                                   >  1:765250/1‑147 (MQ=255)
             caccacACCGCCCGGTATGGATTGCCACCAGCACTCACGACGGCGAAGAGAGTGTGGTGATCGCCGCACATCAGGCATTGTTACAGCAATTCCCGAATTTATTGCTCATCCTGGTACCCCGTCATCCGGAACGCTTCCCGGATGCGAtt                                                                                                                                   >  1:731656/1‑149 (MQ=255)
               caccacCGCCCGGTATGGCTTGCCACCAGCACTCACGAAGGCGAAGAGAGTGTGGTGATCGCCGCACATCAGGCATTGTTCCAGCAATTCACTAATTTATTGCTCATCCTGGTACCCCGTCATCCGGAACGCTTCCCGGATGCGATTaa                                                                                                                                 <  1:67650/146‑1 (MQ=255)
                   acCGCCCGGTATGGATTGCCACCAGCACTCACGAAGGCGAAGAGAGTGTGGTGATCGCCGCACATCAGGCATTGTTACAGCAATTCCCGAATTTATTGCTCATCCTGGTACCCCGTCATCCGGAACGCTTCCCGGATGCGATTAACCtt                                                                                                                             >  2:472830/1‑149 (MQ=255)
                    ccGCCCGGTATGGATTGCCACCAGCACTCACGACGGCGAAGAGAGTGTGGTGATCGCCGCACATCAGGCATTGTTACAGCAATTCCCGAATTTATTGCTCATCCTGGTACCCCGTCATCCGGAACGCTTCCCGGATGCGATTAACCTTg                                                                                                                            <  1:458377/149‑1 (MQ=255)
                     cGCCCGGTATGGATTGCCACCAGCACTCACGAAGGCGAAGAGATTGTGGTGATCTCCGCACATCAGGCATTGTTACAGCAATTCCCGAATTTATTGCTCATCCTGGTACCCCGTCATCCGGAACGCTTCCCGGATGAGATTTACCTTGt                                                                                                                           >  2:504503/1‑149 (MQ=255)
                     cGCCCGGTATGGATTGCCACCAGCACTCACGAAGGCGAAGAGAGTGTGGTGATCGCCGCACATCAGGCATTGTTACAGCAATTCCCGAATTTATTGCTCATCCTGGTACCCCGTCATCCGGAACGCTTCCCGGATGCGATTAACCTTGt                                                                                                                           >  1:981317/1‑149 (MQ=255)
                      gCCCGGTATGGATTGCCACCAGCACTCACGAAGGCGAAGAGAGTGTGGTGATCGCCGCACATCAGGCATTGTTACAGCAATTCCCGAATTTATTGCTCATCCTGGTACCCCGTCATCCGGAACGCTTCCCGGATGCGATTAACCTTGTc                                                                                                                          <  2:215994/149‑1 (MQ=255)
                       cccGGTATGGATTGCCACCAGCACTCACGAAGGCGAAGAGAGTGTGGTGCTCGCCGCGCATCAGGCATTGTTACAGCAATTCCCGAATTTATTGCTCATCCTGGTACCCCGTCATCCGGAACGCTTCCCGGATGCGATTAACCTTCTcc                                                                                                                         >  1:135813/1‑149 (MQ=255)
                          ggTATGGATTGCCACCAGCACTCACGAAGGCGAAGAGAGTGTGGTGATCGCCGCACATCAGGCATTGTTACAGCAATTCCCGAATTTATTGCTCATCCTGGTACCCCGTCATCCGGAACGCTTCCCGGATGCGATTAACCTTGTCCGcc                                                                                                                      <  1:187282/149‑1 (MQ=255)
                           gctaggAATGACACCATCACTCACGATGGCGTAGAGAGTTTCTGGATCGCCGCACAGCAGCCATTGTTACAGCAATTCCCGAATTTCTTGCTCCTCCTGGTACCCCGGCATCGGGAACGCTTCCCGGATGCGATTAACCTTGTCCGCCa                                                                                                                     <  2:748281/145‑1 (MQ=255)
                            tATGGATTGCCACCAGCACTCACGAAGTCGAAGAGAGTGTGGTGATCGCCGCACATCAGGCATTGTTACAGCAATTCCCGAATTTATTGCTCATCCTGGTACCCCGTCATCCGGAACGCTTCCCGGATGCGATTAACCTTGTCCGCCAg                                                                                                                    <  2:697769/149‑1 (MQ=255)
                               ggATTGCCACCAGCACTCACGAAGGCGAAGAGAGTGTGGTGATCGCCGCACAGCAGGCATTGTTACAGCAATTCCCGAATTTATTGCTAATCCTCAAACCCCGTCAACCATAACGATTCACGAATTCTATTAACCTTGACAGCCacaaa                                                                                                                 >  2:680107/1‑145 (MQ=255)
                                gATTGCCACCAGCACTCACGAAGGCGAAGAGAGTGTGGTGATCGCCGCACATCAGGCATTGTTACAGCAATTCCCGAATTTATTGCTCATCCTGGTACCCCGTCANCCGGAACNCTTCCCGGATGCGATTAACCTTGTCCGCCAGGCTg                                                                                                                <  1:356532/149‑1 (MQ=255)
                                gATTGCCACCAGCACTCACGAAGGCGAAGAGAGTGTGGTGATCGCCGCACATCAGGCATTGTTACAGCAAGTCCCGAATTTATTGCTCATCCTGGTACCCCGTCATCCGGAACGCTTCCGGGATGCGATTAACCTTGTCCGCCAGGCTg                                                                                                                >  2:833585/1‑149 (MQ=255)
                                     ccaccaGCACTCACGAAGGCGAAGAGAGTGTGGTGATCGCCGCACATCAGGCATTGTTACAGCAATTCCCGAATTTATTGCTCATCCTGGTACCCCGTCATCCGGAACGCTTCCCGGATGCGATTAACCTTGTCCGCCAGGCTGGACTa                                                                                                           >  1:494328/1‑149 (MQ=255)
                                     ccaccaGCACTCACGAAGGCGAAGAGAGTGTGGTGATCGCCGCACATCAGGCATTGTTACAGCAATTCCCGAATTTATTGCTCATCCTGGTACCCCGTCATCCGGAACGCATCCCGGATGCGATTAACCTTGTCCGCCAGGCTGGACTa                                                                                                           >  2:949395/1‑149 (MQ=255)
                                       cccaGCACTCACGAAGGCGAAGAGAGTGTGGTGCTCGCGGCACATCCGCCATGGGTACAGCAAGTCCCGACGAGCTTGCGCATCCTGGTCCCCCGTCAGCCGGAACATGTCGGTGTTGCTATTAACCTTGTGCGCGAGGCTGGAGTAAg                                                                                                         >  2:673836/2‑149 (MQ=255)
                                       accaGCACTCACGAAGGCGAAGAGAGTGTGGTGATCGCCGCACATCAGGCATTGTTACACCAATTCCCGAATTTATTGCTCATCCTGGTACCCCGTCATCCGGAACGCTTCCCGGATGCGATTAACCTTGTCCGCCAGGCTGGACTAAg                                                                                                         <  1:437796/149‑1 (MQ=255)
                                       accaGCACTCACGAAGGCGAAGAGAGTGTGGTGAACGCCGCACATAATGCATTGTTACAGCAATTCCCGAATTTATTGCTCATCCTGGTACCCCGTCATCCGGAACGCTTCCCGGATGCGATTAACCTTGTCCGCCAGGCTGGACTAAg                                                                                                         <  2:882592/149‑1 (MQ=255)
                                       accaGCACTACCGAAGGCGAAGAGAGTGTGGTGATCGCCGCACATCAGGCATTGTTACAGCAATTCCCGCATTTATTGCTCATCCTGGTACCCCGTCATCCGGAACGCTTCCCGGATGCGATTATCCTTGTCCGCCAGGCTGGACTAAg                                                                                                         >  2:777319/1‑149 (MQ=255)
                                        ccaGCACTCACGAAGGCGAAGAGAGTGTGGTGATCGCCGCACATCAGGCATTGTTACAGCAATTCCCGAATTTATTGCTCATCCTGGTACCCCGTCATCCGGAACGCTTCCCGGATGCGATTAACCTTGTCCGCCAGGCTGGACTAAGc                                                                                                        >  1:153406/1‑149 (MQ=255)
                                         caGCACTCACGAAGGCGAAGAGAGTGTGGTGATCGCCGCACATCAGGCATTGTTACAGCAATTCCCGAATTTATTGCTCATCCTGGTACCCCGTCATCCGGAACGCTTCCCGGATGCGATTAACCTTGTCCGCCAGGCTGGACTAAGCt                                                                                                       >  1:413770/1‑149 (MQ=255)
                                         caGCACTCACGAAGGCGAAGAGAGTGTGGTGATCGCCGCACATCAGGCATTGTTACAGCAATTCCCGAATTTATTGCTCATCCTGGTACCCCGTCATCCGGAACGCTTCCCGGATGCGATTAACCTTGTCCGCCAGGCTGGACTAAGCt                                                                                                       <  1:18265/149‑1 (MQ=255)
                                         caGCACTCACGAAGGCGAAGAGAGTGTGGTGATCGCCGCACATCAGGCATTGTTACAGCAATTCCCGAATTTATTGCTCATCCTGGTACCCCGTCATCCGGAACGCTTCCCGGATGCGATTAACCTTGTCCGCCAGGCTGGACTAAGCt                                                                                                       <  1:711708/149‑1 (MQ=255)
                                            cACTCACGAAGGCGAAGAGAGTGTGGTGATCGCCGCACATCAGGCATTGTTACAGCAATTCCCGAATTTATTGCTCATCCTGGTACCCCGTCATCCGGAACGCTTCCCGGATGCGATTAACCTTGTCCGCCAGGCTGGACTAAGCtata                                                                                                    <  1:984317/149‑1 (MQ=255)
                                              cTCACGAAGGCGAAGAGAGTGTGGTGATCGCCGCACATCAGGCATTGTTACAGCAATTCCCGAATTTATTGCTCATCCTTGTACCCCGTCATCCGGAACGCTTCCCGGATGCGATTAACCTTGTCCGCCAGGCTGGACTAAGCTATATc                                                                                                  <  1:473106/149‑1 (MQ=255)
                                               tCACGAAGGCGAAGAGAGTGTGGTGATCGCCGCACATCAGGCATTGTTACAGCAATTCCCGAATTTATTGCTCATCCTGGTACCCCGTCATCCGGAACGCTTCCCGGATGCGATTAACCTTGTCCGCCAGGCTGGACTAAGCTATATca                                                                                                 >  2:224517/1‑149 (MQ=255)
                                                cACGAAGGCGAAGAGAGTGTGGTGATCGCCGCACATCAGGCATTGTTACAGCAATTCCCGAATTTATTGCTCATCCTGGTACCCCGTCATCCGGAACGCTTCCCGGATGCGATTAACCTTGTCCGCCAGGCTGGACTAAGCTATATcac                                                                                                <  1:886672/149‑1 (MQ=255)
                                                 aCGAAGGCGAAGAGAGTGTGGTGATCGCCGCACATCAGGCATTGTTACAGCAATTCCCGAATTTATTGCTCATCCTGGTACCCCGTCATCCGGAACGCTTCCCGGATGCGATTAACCTTGTCCGCCAGGCTGGACTAAGCTATATcacc                                                                                               >  2:798993/1‑148 (MQ=255)
                                                   gAAGGCGAAGAGAGTGTGGTGATCGCCGCACATCAGGCATTGTTACAGCAATTCCCGAATTTATTGCTCATCCTGGTACCCCGTCATCCGGACCTCGTCCCGGATGCGATTAACCTTGTCCGCCAGGCTGGACTACGCTATATCACACg                                                                                             >  1:745368/1‑149 (MQ=255)
                                                   gAAGGCGAAGAGAGTGTGGTGATCGCCGCACATCAGGCATTGTTACAGCAATTCCCGAATTTATTGCTCATCCTGGTACACCGTCATCCGGAACGCTTCCCGGATGCGATTAACCTTGTCCGCCAGGCTGGACTAAGCTATATCACACg                                                                                             >  1:221137/1‑149 (MQ=255)
                                                   gAAGGCGAAGAGAGTGGGGTGATCGCCGCACATCAGGCATGGTTACAGCAATTCCCGAATTTATTGCTCATCCTGGTACCCCGTCATCCGGAACGCTTCCCGGATGCGATTAACCTTGTCCGCCAGGCTGGACTAAGCTATATCACACg                                                                                             <  2:377037/149‑1 (MQ=255)
                                                        cGAAGAGAGTGTGGTGATCGCCGCACATCAGGCATTGTTACAGCAATTCCCGAATTTATTGCTCATCCTGGTACCCCGTCATCCGGAACGCTTCCCGGATGCGATTAACCTTGTCCGCCAGGCTGGACTAAGCTATATCACACGCTCtt                                                                                        <  2:307909/149‑1 (MQ=255)
                                                         gAAGAGAGTGTGGTGATCGCCGCACATCAGGCATTGTTACAGCAATTCCCGAATTTATTGCTCATCCTGTTACCCCGTCATCCGGAACGCTGCCCGGCTGCGATTTACCTTGTCCGCCAGGCTGGACTTAGCTATATCACACGCTCTTc                                                                                       >  2:314194/1‑149 (MQ=255)
                                                         gAAGAGAGTGTGGTGATCGCCGCACATCAGGCATTGTTACAGCAATTCCCGAATTTATTGCTCATCCTGGTACCCCGTCATCCGGAACGCTTCCCGGATGCGATTAACCTTGTCCGCCAGGCTGGACTAAGCTATATCACACGCTCTTc                                                                                       >  1:809678/1‑149 (MQ=255)
                                                             tgagTGTGGTGATCGCCGCACATCAGGCATTGTTACAGCACTTCCCGAATTTATTGCTCAACCTGGTACCCCGTCATCCGGAACGCTTTCCGGATGCGATTAACCTTGTCCGCCAGGCTGGACTAAGCTATATCACACGCTATTCAggg                                                                                   <  2:283156/148‑1 (MQ=255)
                                                             agagTGTGGTGATCGCCGCACATCAGGCATTGTTACAGCAATTCCCGAATTTATTGCTCATCCTGGTACCCCGTCATCCGGAACGCTTCCCGGATGCGATTAACCTTGTCCGCCAGGCTGGACTAAGCTATATCACACGCTCTTCAggg                                                                                   <  2:392717/149‑1 (MQ=255)
                                                             agagTGTGGTGATCGCCGCACATCAGGCATTGTTACAGCAATTCCCGAATTTATTGCTCATCCTGGTACCCCGTCATCCGGAACGCTTCCCGGATGCGATTAACCTTGTCCGCCAGGCTGGACTAAGCTATATCACACGCTCTTCAggg                                                                                   <  2:372382/149‑1 (MQ=255)
                                                              gtgtgtGGTGATCGCCGCAGATCAGGCATTGTTACAGCAATTCCCGAATTTATTGCTCATCCTGGTACCCCGTCATCCGGAACGCTTCCGGGATGCGATTAACCTTGTCCGCCAGGCTGGACTAAGCTATATCACACGCTCTTCAgggg                                                                                  <  2:983850/147‑1 (MQ=255)
                                                               agTGTGGTGATCGCCGCACATCAGGCATTGTTACAGCAATTCCCGAATTTATTGCTCATCCTGGTACCCCGTCATCCGGAACGCTTCCCGGATGCGATTAACCTTGTCCGCCAGGCTGGACTAAGCTATATCACACGCTCTTCAGGGGa                                                                                 <  1:523928/149‑1 (MQ=255)
                                                                  gtggtgATCGCCGCACATCAGGCATTGTTACAGCAATTCCCGAATTTATTGCTCATCCTGGTACCCCGTCATCCGGAACGCTTCCCGGATGCGATTAACCTTGTCCGCCAGGCTGGACTAAGCTATATCACACGCTCTTCAGGGGAAGt                                                                              <  1:543844/149‑1 (MQ=255)
                                                                   tggtgATCGCCGCACATCAGGCATTGTTACAGCAATTCCCGAATTTATTGCTCATCCTGGTCCCCCGTCCTCCGGAACGCTTCCCGGATGCGATTACCCTTGTCCGCCAGGCTGGACTAAGCTATATCACACGCTCTTCAGGGGAAGTc                                                                             >  1:893890/1‑149 (MQ=255)
                                                                   tggtgATCGCCGCACATCAGGCATTGTTACAGCAATTCCCGAATTTATTGCTCATCCTGGTACCCCGTCATCCGGATCGCTTCCCGGATGCGATTAACCTTGTCCTCCAGGCTGGACTAAGCTATATCACACGCTCTACAGGGGAAGTc                                                                             >  1:313575/1‑149 (MQ=255)
                                                                     gtgATCGCCGCACATCAGGCATTGTTACAGCAATTCCCGAATTTATTGCTCATCCTGGTACCCCGTCATCCGGAACGCTTCCCGGATGCGATTAACCTTGTCCGCCAGGCTGGACTAAGCTATATCACACGCTCTTCAGGGGAAGTccc                                                                           <  2:12606/149‑1 (MQ=255)
                                                                       gATCGCCGCACATCAGGCATTGTTACAGCAATTCCCGAATTTATTGCTCATCCTGGTACCCCGTCATCCGGAACGCTTCCCGGATGCGATTAACCTTGTCCGCCAGGCTGGACTAAGCTATATCACACGCTCTTCAGGGGAAGTccccg                                                                         >  2:884894/1‑148 (MQ=255)
                                                                        aTCGCCGCACATCAGGCATTGTTACAGCAATTCCCGAATTTATTGCTCATCCTGGTACCCCGTCATCCGGAACGCTACCCGGATGCGATTAACCTTGTCCGCCAGGCTGGACTAAGCTATATCACACGCTCTTCAGGGGAAGTCCCCTc                                                                        <  1:560072/149‑1 (MQ=255)
                                                                        aTCGCCGCACATCAGGCATTGTTACAGCAATTCCCGAATTTATTGCTCATCCTGGTACCCCGTCATCCGGAACGCTACCCGGATGCGATTAACCTTGTCCGCCAGGCTGGACTAAGCTATATCACACGCTCTTCAGGGGAAGTCCCCTc                                                                        <  1:154141/149‑1 (MQ=255)
                                                                           gccgcACATCAGGCATTGTTACAGCAATTCCCGAATTTATTGCTCATCCTGGTACACCGTCATCCGGAACGCTTCCCGGATGCGATTAACCTTGTCCGCCAGGCTGGACTAAGCTATATCACACGCTCTTCAGGGGAAGTCCCCCccac                                                                     <  1:504503/149‑1 (MQ=255)
                                                                             cgcACATCAGGCATTGTTACAGCAATTCCCGAATTTATTGCTCCTCCTGGTACCCCGTCATCCGGAACGCTTCCCGGATGCGATTAACCTTGTCCGCCAGGCTGGACTAAGCTATATCACACGCTCTTCAGGGGAAGTCCCCTccacca                                                                   <  2:503723/149‑1 (MQ=255)
                                                                               cacaTCAGGCATTGTTACAGCAATTCCCGAATTTATTGCTCATCCTGGTACCCCTTCATCCGGAACGCTTCCCGGATGCGATTAACCTTGGCCGCCAGGCTGGACTAAGCTATATCACACGCTCTTCAGGGGAAGTCCCCTCCACCAGc                                                                 <  2:731656/149‑1 (MQ=255)
                                                                                acaTCAGGCATTGTTACAGCAATTCCCGAATTTATTGCTCATCCTGGTACCCCGTCATCCGGAACGCTTCCCGGATGCGATTAACCTTGTCCGCCAGGCTGGACTAAGCTATATCCCACGCTCTTCAGGGGAAGGCCCCTCCACCAGcc                                                                >  2:510867/1‑148 (MQ=255)
                                                                                  aTCAGGCATTGTTACAGCAATTCCCGAATTTATTGCTCATCCTGGTACCCCGTCATCCGGAACGCTTCCCGGATGCGATTACCCTTGTCCGCCAGGCTGGACTAAGCTATATCACACGCTCTTCAGGGGAAGTCCCCTCCACCAGCACg                                                              >  2:419025/1‑149 (MQ=255)
                                                                                  aTCAGGCATTGTTACAGCAATTCCCGAATTTATTGCTCATCCTGGTACCCCGTCATCCGGAACGCTTCCCGGATGCGATTAACCTTGTCCGCCAGGCTGGCCTAAGCTATATCACGCGCTCTTCAGGGGAAGTCCCCTCCACCAGCACg                                                              >  1:945343/1‑149 (MQ=255)
                                                                                   tCAGGCATTGTTACAGCAATTCCCGAATTTATTGTTCATCCTGGTACCCCGTCATCCGGAACGCTTCCCGGATGCGATTAACCTTGTCCGCCAGGCTGGACTAAGCTATATCACACGCTCTTCAGGGGAAGTCCCCTCCACCAGCACGc                                                             >  1:890920/1‑149 (MQ=255)
                                                                                   tCAGGCATTGTTACAGCAATTCCCGAATTTATTGCTCATCCTGGAACCCCGTCATCCGGAACGCTTACCGGATGCGATTAACCTTGTCCGCCAGGCTGGACTAAGGTTTATCACACGCTCTTCAGGGGAAGTCCCCTCCACCAGCACGc                                                             >  2:616392/1‑149 (MQ=255)
                                                                                    cAGGCATTGTTACAGCAATACCCGAATTTATAGCTCATCCTGGTACGCCGTCATCCGTAACGCTTCCCGGATGCGATTAACCTTGTCCGCCAGGCTGGACTAAGCTATATCACACGCTCTTCAGGGGAAGTCCCCTCCACCAGCACGct                                                            <  1:52650/149‑2 (MQ=255)
                                                                                      ggCATTGTTACAGCAATTCCCGAATTTATTGCTCATCCTGTTACCCCGTCATCCGGAACGCTTCCCGGATGCGATTAACCTTGTCCGCCAGGCTGGACTAAGCTATATCAAACGCTCTTCACGGGATGTCCCCTCCACACGCACGCAgg                                                          >  2:472848/1‑149 (MQ=255)
                                                                                            gTTACAGCAATTCCCGAATTTATTGCTCATCCTGGTACCCCGTCATCCGGAACGCTTCCCGGATGCGATTAACCTTGTCCGCCAGGCTGGACTAAGCTATATCACCCGCTCTACAGGGGAAGTCCCCTCCACCAGCACGCAGGTTGTgg                                                    >  2:244486/1‑149 (MQ=255)
                                                                                            gTTACAGCAATTCCCGAATTTATTGCTCATCCTGGTACCCCGTCATCCGGAACGCTTCCCGGATGCGATTAACCTTGTCCGCCAGGCTGGACTAAGCTATATCACACGCTCTTCAGGGGAAGTCCCCTCCACCAGCACGCAGGTTGTgg                                                    >  2:248194/1‑149 (MQ=255)
                                                                                             ttACAGCAATTCCCGAATTTATTGCTCATCCTGGTACCCCGTCATCCGGAACGCTTCCCGGATGCGATTACCCTTGTCCGCCAGGCTGGACTAAGCTATATCACACGCTCTTCAGGGGAAGTCCCCTCCACCAGCACGCAGGTTGTGGt                                                   >  2:161009/1‑149 (MQ=255)
                                                                                              tACAGCAATTCCCGAATTTATTGCTCATCCTGGTACCCCGTCATCCGGAACGCTTCCCGGATGCGATTAACCTTGTCCGCCAGGCTGGACTAAGCTATATCACACGCTCTTCAGGGGAAGTCCCCTCCACCAGCACGCAGGTTGTGGtt                                                  <  1:480936/149‑1 (MQ=255)
                                                                                              tACAGCAATTCCCGAATTTATTGCTCATCCTCGTACCCCGTCATCCGGAACGCTTCCCGGATGCGATTAACCTTGTCCGCCAGGCTGGACTAAGCTATATCACACGCTCTTCAGGGGAAGTCCCCTCCACCAGCACGCAGGGTGTGGtt                                                  <  2:66417/149‑1 (MQ=255)
                                                                                               aCAGCAATTCCCGAATTTATTGCTCATCCTGGTACCCCGTCATCCGGAACGCTTCCCGGATGCGATTAACCTTGTCCGCCAGGCTGGACTAAGCTATATCACACGCTCTTCAGGGGAAGTCCCCTCCACCAGCACGCAGGTTGTGGTTg                                                 >  2:813510/1‑149 (MQ=255)
                                                                                               aCAGCAATTCCCGAATATATTGCTCATCCTGGTACCCCGGCATCCGGAACGCTTCCCGGATGCGATTAACCTTGTCCGCCAGGCTGGACTAAGCTATATCACACGCTCTTCAGGGGAAGTCCCCTCCACCAGCACGCAGGTTGTGGTTg                                                 <  2:534498/149‑1 (MQ=255)
                                                                                                 cgCAAATCCCGAATTTATTGCTCATCCTGGTACCCCGTCATCCGGAACGCGTCCCGGATGCGATTAACCTGGTCCGCCAGGCTGGACTACGCTATATCCCACGCTCTTCAGGGGCAGTCCCCTCCCCCCGCCCGCAGGTTGTGGTTGGc                                               <  2:970587/148‑1 (MQ=255)
                                                                                                 aGCAATTCCCGAATTTATTGCTCATCCTGGTACCCCGTCATCCGGAACGCTTCCCGGATGCGATTAACCTTGTCCGCCAGGCTGGACTAAGCTATATCACCCGCTCTTCAGGGGAAGTCCCCTCCACCAGCACGCAGGTTGTGGTTGGc                                               <  2:118800/149‑1 (MQ=255)
                                                                                                 aGCAATTCCCGAATTTATTGCTCATCCTCGTACCCCGTCATCCGGAACCCTTCCCGGAACAGAATAACCTTGTCCGACAGGCTGGACTAAGCAAAATCACAAGCTCTTCTGCGGAAGTCCCCTCCACCAGCACGCAGGTTGTGGATGGc                                               >  1:589444/1‑149 (MQ=255)
                                                                                                  gCAATTCCCGAATTTATTGCTCATCCTGGTACCCCGTCATCCGGAACGCTTCCCGGATGCGATTAACCTTGTCCGCCAGGCTGGACTAAGCTATATCACACGCTCTTCAGGGGAAGTCCCCTCCCCCAGCACGCAGGTTGGGGTTGGCg                                              >  2:775734/1‑149 (MQ=255)
                                                                                                         ccGAATTTATTGCTCATCCTGGTACCCCGTCATCCGGAACGCTTCCCGGATGCGATTAACCTTGTCCGCCAGGCTGGACTAAGCTATATCACACGCTCTTCAGGGGAAGTCCCCTCCCACAGCACGCAGGTTGTGGTGGGCGATACGAt                                       >  2:382512/1‑149 (MQ=255)
                                                                                                         ccGAATTTATTGCTCATCCTGGTACCCCGTCATCCGGAACGCTTCCCGGATGCGATTAACCTTCTCCGCAAGGCTGGACTAAGCTAGATCACACGCTCTTCAGGGGAAGACCCCTCCAGCAGCACGCAGGGGGTGGTAGGCGAGACGAt                                       >  1:669703/1‑149 (MQ=255)
                                                                                                          cGAATTTATTGCTCATCCTGGTACCCCGTCATCCGGAACGCTTCCCGGATGCGATTAACCTTGTCCGCCAGGCTGGACTAAGCTATATCACACGCTCTTCAGGGGAAGTCCCCTCCACCAGCACGCAGGTTGTGGTTGGCGATACGATg                                      >  1:838818/1‑149 (MQ=255)
                                                                                                           gAATTTATTGCTCATCCTGGTACCCCGTCATCCGGAACGCTACCCGGATGCGATTAACCTTGTCCGCCAGGCTGGACTAAGCTATATCACACGCTCTTCAGGGGAAGTCCCCTCCACCAGCACGCAGGTTGGGGGTGGCGATAGGATgg                                     >  2:464770/1‑149 (MQ=255)
                                                                                                            aaTTAAATGCGCATCCTGGTACCCCGTCATCCGGAACGCTTCCCGGATGCGATTAACCTTGTCCGCCAGGCTGGACTAAGCTATATCACACGCTCTTCAGGGGAAGTCCCCTCCACCAGCACGCAGGTTGTGGTTGGCGTTACGATggg                                    <  1:1033250/149‑1 (MQ=255)
                                                                                                                    gCTGATCCTGGTACCCCGTCCTCCGGAACGCTTCCCGGATGCGATTAACCTTGACCGCCAGGCTGGACTAAGCTATATCACACGCTCTTCAGGTGAAGTCTCATCCACCAGCTCGGAGGTTGTGGTTGGCGATACGATGTGCGAGTTGa                            <  2:765250/149‑1 (MQ=255)
                                                                                                                    gCTCATCCTGGTACCCCGTCATCGGGAACGCTTCCCGGATGCGATTAACCTTGTCCGCCAGGCTGGACTAAGCTATATCACACGCTCTTCAGGGGAAGTCCCCTCCACCAGCACGCAGGGTGTGGTTGGCGATACGATGGGCGAGTTGa                            <  1:814382/149‑1 (MQ=255)
                                                                                                                    gCTCATCCTGGTACCCCGTCATCCGGAACGCTTCCCGGATGCGATTAACCTTGTCCGCCAGGCTGGACTAAGCTATATCACACGCTCTTCAGGGGAAGTCCCCTCCACCAGCACGCAGGTTGTGGTTGGCGATACGATGGGCGAGTTGa                            <  1:161009/149‑1 (MQ=255)
                                                                                                                    gCTCATCCTGGTACACCGTCATACGGAACGCTTCCCGGATGCGATTACACTTGTCCTCCAGGCTGGACTAAGCGATATAACACGCACTTCAGAGGAAGTCCCCTCAACCAGCACGCAGTGTGTGGTTGGCGATCCGATGGGCTAGTGGa                            >  2:927021/1‑149 (MQ=255)
                                                                                                                     cTCATCCTGGTACCCCGTCATCCGGCACGCTTCCCGGATGCGATTAACCTTGTCCGCCAGGCTGGACTATGCTATATCACACGCTCTTCAGGGGAAGTCCCCTGCACCATCACGCAGGTTGTGGTGGGCGATACGATGTGAGAGTTGAt                           >  1:583607/1‑149 (MQ=255)
                                                                                                                     cTCATCCTGGTACACCGTCATCCGGAACGCTTCCCGGATGCGATTAACCTTGTCCGCCAGGCTGGACTAAGCTATATCACACGCTCTTCAGGGGAAGTCCCCTCCACCAGCACGCAGGATGAGGTTGGCGATACGATGGGCGAGTTGAt                           >  2:367828/1‑149 (MQ=255)
                                                                                                                       cATCCTGGTACCCCGTCATCCGGAACGCTTCCCGGATGCGATTAACCTTGTCCGCCAGGCTGGACTAAGCTATATCACACGCTCTTCAGGGGAAGTCCCCTCCACCAGCACGCAGGTTGTGGTTGGCGATACGATGGGCGAGTTGATGt                         <  1:126565/149‑1 (MQ=255)
                                                                                                                         tgcTGGGACCCCCGCCTCCGGAACGGTTGCCGGTTGGGGTTAACCTTTTGCGCCATGCTGGTCTTAGCTATATCACACGCTCTTCAGGGGAAGTCCCCTCCACCAGCACGCAGGTTGTGGTTGGCGATACGATGGGCGAGTTGATGTTa                       <  2:981317/147‑1 (MQ=255)
                                                                                                                           cTGGTACCCCGTCATCCGGAACGCTTCCCGGATGCGATTAACCTTGTCCGCCAGGCTGGACTAAGCTATATCACACGCTCTTCAGGGGAAGTCCCCTCCACCAGCACGCAGGTTGTGGTTGGCGATACGATGGGCGAGTTGATGTTACt                     <  1:454879/149‑1 (MQ=255)
                                                                                                                                 ccccGTCATCCGGAACGCTTCCCGGATGCGATTAACCTTGACCGCCAGGCTGGACTAAGCTATATCACACGCTCTTCAGGGGAAGTCCCCTCCACCTGCACGCAGCTTGTGGTTGGCGATACGATGGGCGCCTTGATGTTACTGTATgg               >  1:844820/1‑149 (MQ=255)
                                                                                                                                   ccGTCATCCGGAACGCTTGCCGGATGCGATTAACCTTGTCCGCCAGGCTGGACTAAGCTATATCACACGCTCTTCAGGGGAAGTCCCCTCCACCAGCACGCAGGTTGTGGTTGGCGATTCGATGGGCGAGTTGATGTTACTGTATGGCa             >  1:717165/1‑149 (MQ=255)
                                                                                                                                    tcgcATCCGGGAAGCGTTCCGGATGCGATTAACCTTGTCCGCCAGGCTGGACTAAGCTATATCACACGCTCTTCAGGGGAAGTCCCCTCCACCAGCACGCAGGTTGTGGTTGGCGATACGATGGGCGAGTTGATGTTACTGTATGGCAt            >  1:868466/4‑149 (MQ=255)
                                                                                                                                     cgcATCCGGGAAGCGTTCCGGATGCGATTAACCTTGTCCGCCAGGCTGGACTAAGCTATATCACACGCTCTTCAGGGGAAGTCCCCTCCACCAGCACGCAGGTTGTGGTTGGCGATACGATGGGCGAGTTGATGTACTGTATGGCATTg          >  2:777444/3‑149 (MQ=255)
                                                                                                                                        aTCCGGGAAGCGTTCCGGATGCGATTAACCTTGTCCGCCAGGCTGGACTAAGCTATATCACACGCTCTTCAGGGGAAGTCCCCTCCACCAGCACGCAGGTTGTGGTTGGCGATACGATGGGCGAGTTGATGTTACTGTATGGCATTGcc        >  2:262695/1‑149 (MQ=255)
                                                                                                                                         tCCGGAACGCTTCCCGGATGCGATTAACCTTGTCCGCCAGGCTGGACTAAGCTATATCCCACGCTCTTCAGGGGAAGTCCCCTCCACCAGCACGCAGGTTGTGGTTGGCGATACGATGGGCGAGTTGATGTTACTGTATGGCATTGCCg       >  1:202809/1‑149 (MQ=255)
                                                                                                                                           cGGAAAGCTTCCCGGATGCGATTATCCTTGAGCGCCCGGCTGGACTAAGCTATAGCACCCGCTCTTCAGGGGAAGTCCCCTCCACCAGCACGCAGGTTGTGGTTGGCGATTCGATGGGCGAGTTGATGTTACTGTATGGCATTGCCGAt     <  2:442811/149‑1 (MQ=255)
                                                                                                                                              aaCGCTTCCCGGATGCGATTAACCTTGTCCGCCAGGCTGGACTAAGCTATATCACACGCTCTTCAGGGGAAGTACCCTCCACCAGCACGCAGGTTGTGGTTGGCGATACGATGGGCGAGTTGATGTTACTGTATGGCATTGCCGAtctc  <  2:413770/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                               
CGCCGCCAGTGGGCACCACACCGCCCGGTATGGATTGCCACCAGCACTCACGAAGGCGAAGAGAGTGTGGTGATCGCCGCACATCAGGCATTGTTACAGCAATTCCCGAATTTATTGCTCATCCTGGTACCCCGTCATCCGGAACGCTTCCCGGATGCGATTAACCTTGTCCGCCAGGCTGGACTAAGCTATATCACACGCTCTTCAGGGGAAGTCCCCTCCACCAGCACGCAGGTTGTGGTTGGCGATACGATGGGCGAGTTGATGTTACTGTATGGCATTGCCGATCTC  >  NC_000913/3809212‑3809502

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: