Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,953,432 C→T 7.7% intergenic (+19/‑46) ilvE → / → ilvD branched‑chain amino acid aminotransferase/dihydroxyacid dehydratase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,953,4320CT 7.7% 161.9 / 2.2 68intergenic (+19/‑46)ilvE/ilvDbranched‑chain amino acid aminotransferase/dihydroxyacid dehydratase
Reads supporting (aligned to +/- strand):  ref base C (30/29);  new base T (2/3);  total (35/33)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GTAGACGGTATTCAGGTTGGCGAAGGCCGTTGTGGCCCGGTTACCAAACGCATTCAGCAAGCCTTCTTCGGCCTCTTCACTGGCGAAACCGAAGATAAATGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGACACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGCCACCACCACTCATGGTCGTAATATGGCGGGTGCTCGTGCGCTGTGGCGCGCCACCGGAATGACCGACGCCGATTTCGG  >  NC_000913/3953285‑3953575
                                                                                                                                                   |                                                                                                                                               
gTAGACGGTATTCAGCTTGGCGAAGGCCGTTGTGGCCCGGTTACCAAACGCATTCAGCAAGCCTTGTTCGGCCTCTTCACTGGCGAAACCGAAGAGAAATGGGGCTGGGTAGATCAAGTTAAGCATTAAATACAAAAAATGGGACGgca                                                                                                                                                >  1:355226/1‑149 (MQ=255)
  aGACGGTATTCAGGTTGGCGAAGGCCGTTGTGGCCCGGTTACCAAACGCATTCAGCAAGCCTTCTTCGGCCTCTTCACTGGCGAAACCGAAGATAAATGGGGCTGGGTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGgcacg                                                                                                                                              >  2:749263/1‑149 (MQ=255)
    aCGGTATTCAGGTTGGCGAAGGCCGTTGTGGCCCGGTTACCAAACGCATTCAGCAAGCCTTCTTCGGCCTCTTCACTGGCGAAACCGAAGATAAATGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGgcacgca                                                                                                                                            >  2:930207/1‑149 (MQ=255)
    aCGGTATTCAGGTTGGCGAAGGCCGTTGTGGCCCGGTTACCAAACGCATTCAGCAAGCCTTCTTCGGCCTCTTCACTGGCGAAACCGAAGATAAATGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGgcacgca                                                                                                                                            >  1:215141/1‑149 (MQ=255)
         aTTCAGGTTGGCGAAGGCCGTTGTGGCCCGGTTACCAAACGCATTCAGCAAGCCTTCTTCGGCCTCTTCACTGTCGAAACCGAAGATAAATGGGGCTGTTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTc                                                                                                                                       >  1:392865/1‑149 (MQ=255)
         aTTCAGGTTGGCGAAGGCCGTTGTGGCCCGGTTACCAAACGCATTCAGCAAGCCTTCTTCGGCCTCTTCACTGGCGAAACCGAAGATAAATGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTc                                                                                                                                       >  2:258802/1‑149 (MQ=255)
           tccggTAGGCGAAGGCCGTTGTGGCCCGGTTACCAAACGCATTCATCAAGCCTTCTTCGGCCTCTTGACTGGCGAAACCGAAGATAACTGGGGCTGGATAGATCACGTTAATCAATAAATACAAAAAATGGGACGGTGCGCGCCGTccc                                                                                                                                     <  2:635495/146‑1 (MQ=255)
           tCAGGTTGGCGAAGGCCGTGGTGGCCCGGTTACCAAACGCATTCAGCAAGCCTTCTTCGGCCTCTTCACTGGCGAAACCGAAGATAAATGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTccc                                                                                                                                     <  2:113789/149‑1 (MQ=255)
            catgTTGGCGAAGGCCGTTGTGGCCCGGTTAACAAAAGCATTCAGCAAGCCTTCTTCGGCCTCTTCACTGGCTAAACCGAAGATAAATGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCa                                                                                                                                    <  1:652343/146‑1 (MQ=255)
            cAGGTTGGCGAAGGCCGTTGTGGCCCTGTTACCAAACGCATTCAGCAAGCCTTCTTCGGCCTCTTCACTGGCGAAACCGAAGATAAATGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCa                                                                                                                                    <  1:655707/149‑1 (MQ=255)
            cAGGTTGGCGAAGGCCGTTGTGGCCCGGTTACCAAACGCATTCAGCAAGCCTTCTTCGGCCTCTTCACTGGCGAAACCGAAGATAAATGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCa                                                                                                                                    >  2:386326/1‑149 (MQ=255)
            aaGGTTGGCGAAGGCCGTTGTGGCACGGTTACCAAACGCTTTCAGCAAGCCTTCTTCGTCCTCTTCACTGGCGAAACCGAAGATAAATGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGTGCGTGCCGTCCCa                                                                                                                                    <  1:916738/148‑1 (MQ=255)
              ggTTGGCGAAGGCCGTTGTGGCCCGGTTACCAAACGCATTCAGCAAGCCTTCTTCGGCCTCTTCACTGGCGAACCCGAAGATAAATGGGGCTGGTGAGCAGAAGGGAATCAATTAATAAAAATAATTGGACGGACCGCTCCGTACCagg                                                                                                                                  >  1:694221/1‑147 (MQ=255)
               gTTGGCGAAGGCCGTTGTGGCCCGGTTACCAAACGCATTCAGCAAGCCTTCTTCGGCCTCTTCACTGGCGAAACCGAAGATAAATGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCAttt                                                                                                                                 >  2:164138/1‑149 (MQ=255)
                  ggCGAAGGCCGTTGTGGCCCGGTTACCAAACGCATTCAGCAAGCCTTCTTCGGCCTCTTCACTGGCGAAACCGAAGATAAATGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGTGCGTGCCGTCCCAtttttt                                                                                                                              <  2:710643/149‑4 (MQ=255)
                    cGAAGGCCGTTGTGGCCCGGTTACCAAACGCATTCAGCAAGCCTTCTTCGGCCTCTTCACTGGCGAAACCGAAGATAAATGGGGCTGGTTAGAACAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACgag                                                                                                                            <  1:73660/149‑1 (MQ=255)
                           cGTTGTGGCCCGGTTACCAAACGCATTCAGCAAGCCTTCTTCGGCCTCTTCACTGGCGAAAGCGAAGTTAAATGTGGCTGGTTAGATCAAGTTAATCAATAAATACTAAAAATGGGACGGCACGCACCGTCGCATTTACGAgccagaca                                                                                                                     >  1:598669/1‑149 (MQ=255)
                              tgtgGCCCGGTTACCAAACGCATTCAGCAAGCCTTCTTCGGCCTCTTCACTGGCGAAACCGAAGATAAATGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGACACTg                                                                                                                  <  2:140998/149‑1 (MQ=255)
                               gtgGCCCGGTTACCAAACGCATTCAGCAAGCCTTCTTCGGCCTCTTCACTGGCGAAACCGAAGATAAATGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGACACTgg                                                                                                                 <  1:253774/149‑1 (MQ=255)
                               gtgGCCCGGTTAACAAACGCATTCAGCAAGCCTTCTTCGGCCGCTTCACTGGCGTATCCGAAGATAATTGGGGCTGGTTAGATCAAGTTAATCAATAATTACAAAAATTGGGAGGGCAGGCACCGTCCCATTTACGAGACAGACACTgg                                                                                                                 <  2:900157/149‑1 (MQ=255)
                                  gCCCGGTTACCAAACGCATTCAGCAAGCCTTCTTCGGCCTCTTCACTGGCGAAACCGAAGATAAATGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCAGTTACGAGCCAGCCACTGGGAg                                                                                                              >  1:423689/1‑149 (MQ=255)
                                    ccGGTTACCAACCGCATTCAGCAAGCCTTCTTCGGCCTCTTCCCTGGCGAAACCGAAGATAAATGGGGCGGGTTAGATCAAGTTAAGCAATAAATACAAAAAATGGGACGGGACGACCCGTCCCAGTTACGAGCCTGACACTGGGAgga                                                                                                            >  2:679327/1‑147 (MQ=255)
                                     cGGTTACCAAACGCATTCAGCAAGCCTTCTTCGGCCTCTTCACTGGCGAAACCGAAGATAAATGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGACACTGGGAGtaa                                                                                                           <  1:258802/149‑1 (MQ=255)
                                          aCCAAACGCATTCAGCAAGCCTTCTTCGGCCTCTTCACTGGCGAAACCGAAGATAAATGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGACACTGGGAGtaaataaa                                                                                                      <  2:156319/149‑1 (MQ=255)
                                           ccAAACGCATTCAGCAAGCCTTCTTCTGCCTCTTCACTGGCGAAACCGAAGATAAATGGGGCTGGTTAGATCAAGTTAATCAATAAAAACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGACACTGGGAGTAAATAAAg                                                                                                     <  2:937235/149‑1 (MQ=255)
                                            cAAACGCATTCAGCAAGCCTTCTTCGGCCTCTTCACTGGCGAAACCGAAGATAAATGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGACACTGGGAGTAAATAAAg                                                                                                     >  1:643692/1‑148 (MQ=255)
                                            cAAACGCATTCAGCAAGCCTTCTTCGGCCTCTTCACTGGCGAAACCGAAGATAAATGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGACACTGGGAGTAAATAAAg                                                                                                     <  2:643692/148‑1 (MQ=255)
                                            cAAACGCATTCAGCAAGCCTTCTTCGGCCTCTTCACTGGCGAAACCGAAGATAAATGGGGCTGGTAAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGACACTGGGAGTAAATAAAGt                                                                                                    >  1:82599/1‑149 (MQ=255)
                                             aaaCGCATTCAGCAAGCCTTCTTCGGCCTCTTCACTGGCGAAACCGAAGATAAATGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGACACTGGGAGTAAATAAAGTa                                                                                                   <  1:137478/149‑1 (MQ=255)
                                                    ttCAGCAAGCCTTCTTCGGCCTCTTCACTGGCGAAACCGAAGATAAATGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGACACTGGGAGTAAATAAAGTATGCCTaa                                                                                            >  1:520494/1‑149 (MQ=255)
                                                        ggaaGCCTTCTTCGGCCTCTTCTCTGGCGAAACCGAAGATAAATGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGACACTGTGAGTAAATAAAGTATGCCTAAGTAc                                                                                        <  2:356232/147‑1 (MQ=255)
                                                        gCAAGCCTTCTTCGGCCTCTTCACTGGCGAACCCGAAGATAAATGGGGCTGGTTAGATCAAGTTACTCAATAAATACAAAAAATGGGACGGCACGCACCGACCCATTTACGAGAAAGACACTGGGAGTAAATAAAGTATGACTAAGTAc                                                                                        >  2:569866/1‑149 (MQ=255)
                                                         ctatcattcttcTGCCTATTCACTGGCGAAACCGAAGATAAATGGGGCTGGTTAGATAAAGTTAATCAATAAATACAAAAAATGGTACGGCACGCACCGTCCCATTTACGAGACAGACACTGGGAGTAAATAAAGTATGCCTAAGTAcg                                                                                       <  2:949848/143‑2 (MQ=255)
                                                                tcttcGGCCTCTTCACTGGCGAAACCGAAGATAAATGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGACACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGc                                                                                <  2:711253/149‑1 (MQ=255)
                                                                      gCCTCTTCACTGGCGAAACCGAAGATAAATGTGGCTGGTTAGAACAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGACACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGccaccac                                                                          <  1:872349/149‑1 (MQ=255)
                                                                      gCCTCTTCACTGGCGAAACCGAAGATAAATGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGACACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCTccaccac                                                                          >  2:40516/1‑149 (MQ=255)
                                                                       ccTCTTCACTGGCGAAACCGAAGATAAATGGGGCTGGTTAGATCAACTTAAACAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGGCACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTACTccaccacc                                                                         >  1:323217/1‑149 (MQ=255)
                                                                        ctctTCACTGGCGAAACCGATGAAAAATGGGGCTGGTTAGATCAAGTTAATCAATTAATACAAAAAATGGGACGTAACGAACAGTCCAATTTACGAGACAGACACTGGGAGTTAATAAAGTATGCCTAAGTAGCGTTCCGccaccacca                                                                        >  2:730953/1‑149 (MQ=255)
                                                                               cTGGCGAAACCGAAGATAAATGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGACACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGCCACCACCACTCATgg                                                                 <  2:498770/149‑1 (MQ=255)
                                                                               cTGGCGAAACCGAAGATAAATGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGACACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGCCACCACCACTCATgg                                                                 >  2:444042/1‑149 (MQ=255)
                                                                                     aaaCCGAATATAATTGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATTGGACGGCACGCACCGTCCCATTTACGAGACAGACACTGGGATTAAATAAAGTATGCCTAAGTTCCGTTCCGCCACCACCACTCATGGGCGTaa                                                           <  2:211277/149‑1 (MQ=255)
                                                                                          gAAGATAAATGGGGCTGGTTAGATCAAGATAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGACACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGCCACCACCACTCATGGTCGTAATATgg                                                      <  1:340694/149‑1 (MQ=255)
                                                                                          gAAGATAAATGGGGCTGGTTAGAACAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTAACATTTACGAGACAGACACTGGGAGTAAATAAAGTATGCCTAAGAACCGTTCCGCCACCACCACTAAAGGTCGTAATATgg                                                      >  1:43204/1‑149 (MQ=255)
                                                                                                      ggCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGACACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGCCACCACCACTCATGGTCGTAATATGGCGGGTGATCGTg                                          >  2:72583/1‑149 (MQ=255)
                                                                                                          ggTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGACACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGCCACCACCACTCATGGTCGTAATATGGCGGGTGCTCGTGCGCt                                      >  2:436126/1‑149 (MQ=255)
                                                                                                            ttAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGCCACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGCCACCACCACTCATGGTCTTAATATGGCGGTTGCTCCTGCACttt                                    >  2:198333/1‑147 (MQ=255)
                                                                                                             tAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGACACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGCCACCACCACTCATGGTCGTAATATGGCGGGTGCTCGTGCGCtgtg                                   <  1:930207/149‑1 (MQ=255)
                                                                                                             tAGATCAAGCTAATCAATAAATACAAAAACTGGGACGGCACGCACCGTCCCATTTACGAGACAGACACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGCCACCACCACTCATGGTCGTAATATGGCGGGTGCTCGTGCGCtgtg                                   <  2:4044/149‑1 (MQ=255)
                                                                                                                aTCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACACACACTGGGAGTAAATAAAGTATGCCTAAGTACCGTACCGCCACCACCACTCATGGTCGTAATATGGCGGGTGCTCGTGCGCTGTGgcg                                >  2:140393/1‑149 (MQ=255)
                                                                                                                 tCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGACACTGGGAGTAAATAAAGTATGCCTAAGTTCCGTTCCGCCACCACCACTCATGGTCGTAATATGGCGGGTGCTCGTGCGCTGTGgcgc                               <  2:347620/149‑1 (MQ=255)
                                                                                                                   aaGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGACACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGCCACCACCACTCATGGTCGTAATATGGCGGGTGCTCGTGCGCTGTGgcgcgc                             >  1:913364/1‑149 (MQ=255)
                                                                                                                    aGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGACACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGCCACCACCACTCATGGTCGTAATATGGCGGGTGCTCGTGCGCTGTGGCGCGcc                            >  1:6623/1‑149 (MQ=255)
                                                                                                                     gTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGACACTGGGAGTAAATAAAGTAAGCCTAAGTACCGTACCGCCACCACCCCGCATGGGCGAAATATGGCGGCGGATCTTGCGCTGTGGCGCGGCa                           >  2:421997/1‑149 (MQ=255)
                                                                                                                        aaTCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGCCACTGGGAGTAAATAAAGTATGCCTCAGTACCGTTCCGCCACCCCCCCTCATGGTCGTAATAGGGCGGGTGCTCGTGCGCTGTGGCGCGCCCCCg                        >  2:271719/1‑149 (MQ=255)
                                                                                                                        aaTCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGACACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGCCACCACCACTCATGGTCGTAATATGGCGGGTGCTCGTGCGCTGTGGCGCGCCACCg                        >  2:403789/1‑149 (MQ=255)
                                                                                                                          tCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGACACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGCCACCACCACTCATGGTCGTAAGATGGCGGGTGCTCGTGCGCTGTGGCGCGCCACCGGa                      >  2:337372/1‑149 (MQ=255)
                                                                                                                           cAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGACACTGGGAGTAATTAAAGTATGCCTAAGTACCGTTCCGCCCCCACCACTCATGGTCGGAATATGGCGGGTGCTCGTGCGCTGTGGCGCGCCACCGGaa                     >  2:291169/1‑149 (MQ=255)
                                                                                                                           cAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGACACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGCCACCACCACTCATGGTCGTAATATGGCGGGTGCTCGTGCGCTGTGGCGCGCCACCGGaa                     <  1:749263/149‑1 (MQ=255)
                                                                                                                           cAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGACACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGCCACCACCACTCATGGTCGTAATATGGCGGGTGCTCGTGCGCTGTGGCGCGCCACCGGaa                     <  1:805093/149‑1 (MQ=255)
                                                                                                                            aataaataCAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGACACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGCCACCACCACTCATGGTCGTAATATGGCGGGTGCTCGTGCGCTGTGGCGCGCCACCGGAAt                    <  2:392865/149‑1 (MQ=255)
                                                                                                                             ataaataCAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGACACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGCCACCAACACTCATGTTCGTAATATGGCTGGTGATCGTGCGCTGTGGCGCGCCACCGGAATg                   >  2:995742/1‑149 (MQ=255)
                                                                                                                               aaataCAAAAAATGGGACGGCCCGCACCGTCCCATTTACGAGACAGACACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGCCACCACCACTCATGGTCGTAATATGGCGGGTGCTCGTGCGCTGTGGCGCGCCACCGGAATGAc                 <  2:114310/149‑1 (MQ=255)
                                                                                                                                aataCAAAAAATGGGACCGCACGGACCGGCCCATTTACGAGACAGACACTGGGAGTAACTAAAGTATGCCTAAGTACCGTTCCGCCACCACCACTCATGGTCGTAATATGGCGGGTGCTCGTGCGCTGTGGCGCGCCACCGGAATGAcc                <  2:1029245/149‑1 (MQ=255)
                                                                                                                                 ataCAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGACACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGCCACCACCACTCATGGTCGTAATATGGCGGGTGCTCGTGCGCTGTGGCGCGCCACCGGAATGACCg               >  1:122440/1‑149 (MQ=255)
                                                                                                                                   aCAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGACACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGCCACCACCACTCATGGGCGTATTATGGCGGGTGCTCGTGCGCTGTGGCGCGCCACCGGAATGACCGAc             >  1:154357/1‑149 (MQ=255)
                                                                                                                                     aaaaaaTGGTACGGGACGCCACGTCCCATTTACGAGACAGGCACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCTCCACCACCACTCATGGTCGTAATATGGCGGGTGCTCGTGCGCTGTGGCGCGCCACCGGAATGACCTACGc           <  2:323217/149‑1 (MQ=255)
                                                                                                                                     aaaaaaTGGGACGGCACGCACCGTCCCATTTACGAGACAGACACTGGGAGTAAATAAAGTATGCGTAAGTACCGTTCCGCCACCACCACTCATGGTCGTAATATGGCGGGTGCTCGTGCGCTGTGGCGCGCCTCCGGAATGAGCGCCgg           >  2:421843/1‑148 (MQ=255)
                                                                                                                                        aaaTGGGACGGCACGCACCGTCCCATTTACGAGACAGACACTGGGAGTAAATAAAGTATGCCTAAGTCCCGTTCCGCCACCACCACTCATGGTCGTAATATGGCGGGTGCTCGTGCGCTGTGGCGCGCCCCCGGAATGACCGACGCCGa        >  1:97957/1‑149 (MQ=255)
                                                                                                                                          aTGGGACGGTGCGTGCCGTCCCATTTACGAGACAGACACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGCCACCACCACTCATGGTCGTAATATGGCGGGTGCTCGTGCGCTGTGGCGCGCCACCGGAATGTCCGACGCCGAtt      >  2:157121/1‑149 (MQ=255)
                                                                                                                                          aTGGGACGGTGCGTGCCGTCCCATTTACGAGACAGACACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGCCACCACCACTCATGGTCGTAATATGGCGGGTGCTCGTGCGCTGTGGCGCGCCACCGGAATGACCGACGCCGAtt      >  2:645072/1‑149 (MQ=255)
                                                                                                                                            gggACGGCACGCACCGTCCCATTTACGAGACAGACACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGCCACCACCACTCATGGTCGTAATATGGCGGGTGCTCGTGCGCTGTGGCGCGCCACCGGAATGACCGACGCCGATTTc    <  2:82599/149‑1 (MQ=255)
                                                                                                                                              gACGGCACGCACCGTCCCATTTACGAGACAGACACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGCCACCACCACTCATGGTCGTAATATGGCGGGTGCTCGTGCGCTGTGGCGCGCCACCGGAATGACCGACGCCGATTTCgg  <  1:386326/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                               
GTAGACGGTATTCAGGTTGGCGAAGGCCGTTGTGGCCCGGTTACCAAACGCATTCAGCAAGCCTTCTTCGGCCTCTTCACTGGCGAAACCGAAGATAAATGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGACACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGCCACCACCACTCATGGTCGTAATATGGCGGGTGCTCGTGCGCTGTGGCGCGCCACCGGAATGACCGACGCCGATTTCGG  >  NC_000913/3953285‑3953575

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑