Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,953,436 C→T 6.2% intergenic (+23/‑42) ilvE → / → ilvD branched‑chain amino acid aminotransferase/dihydroxyacid dehydratase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,953,4360CT 6.2% 168.5 / 2.3 68intergenic (+23/‑42)ilvE/ilvDbranched‑chain amino acid aminotransferase/dihydroxyacid dehydratase
Reads supporting (aligned to +/- strand):  ref base C (31/30);  new base T (2/2);  total (35/33)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.46e-01

ACGGTATTCAGGTTGGCGAAGGCCGTTGTGGCCCGGTTACCAAACGCATTCAGCAAGCCTTCTTCGGCCTCTTCACTGGCGAAACCGAAGATAAATGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGACACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGCCACCACCACTCATGGTCGTAATATGGCGGGTGCTCGTGCGCTGTGGCGCGCCACCGGAATGACCGACGCCGATTTCGGTAAGCCG  >  NC_000913/3953289‑3953582
                                                                                                                                                   |                                                                                                                                                  
aCGGTATTCAGGTTGGCGAAGGCCGTTGTGGCCCGGTTACCAAACGCATTCAGCAAGCCTTCTTCGGCCTCTTCACTGGCGAAACCGAAGATAAATGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGgcacgca                                                                                                                                                   >  1:215141/1‑149 (MQ=255)
aCGGTATTCAGGTTGGCGAAGGCCGTTGTGGCCCGGTTACCAAACGCATTCAGCAAGCCTTCTTCGGCCTCTTCACTGGCGAAACCGAAGATAAATGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGgcacgca                                                                                                                                                   >  2:930207/1‑149 (MQ=255)
     aTTCAGGTTGGCGAAGGCCGTTGTGGCCCGGTTACCAAACGCATTCAGCAAGCCTTCTTCGGCCTCTTCACTGTCGAAACCGAAGATAAATGGGGCTGTTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTc                                                                                                                                              >  1:392865/1‑149 (MQ=255)
     aTTCAGGTTGGCGAAGGCCGTTGTGGCCCGGTTACCAAACGCATTCAGCAAGCCTTCTTCGGCCTCTTCACTGGCGAAACCGAAGATAAATGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTc                                                                                                                                              >  2:258802/1‑149 (MQ=255)
       tccggTAGGCGAAGGCCGTTGTGGCCCGGTTACCAAACGCATTCATCAAGCCTTCTTCGGCCTCTTGACTGGCGAAACCGAAGATAACTGGGGCTGGATAGATCACGTTAATCAATAAATACAAAAAATGGGACGGTGCGCGCCGTccc                                                                                                                                            <  2:635495/146‑1 (MQ=255)
       tCAGGTTGGCGAAGGCCGTGGTGGCCCGGTTACCAAACGCATTCAGCAAGCCTTCTTCGGCCTCTTCACTGGCGAAACCGAAGATAAATGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTccc                                                                                                                                            <  2:113789/149‑1 (MQ=255)
        catgTTGGCGAAGGCCGTTGTGGCCCGGTTAACAAAAGCATTCAGCAAGCCTTCTTCGGCCTCTTCACTGGCTAAACCGAAGATAAATGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCa                                                                                                                                           <  1:652343/146‑1 (MQ=255)
        cAGGTTGGCGAAGGCCGTTGTGGCCCTGTTACCAAACGCATTCAGCAAGCCTTCTTCGGCCTCTTCACTGGCGAAACCGAAGATAAATGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCa                                                                                                                                           <  1:655707/149‑1 (MQ=255)
        cAGGTTGGCGAAGGCCGTTGTGGCCCGGTTACCAAACGCATTCAGCAAGCCTTCTTCGGCCTCTTCACTGGCGAAACCGAAGATAAATGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCa                                                                                                                                           >  2:386326/1‑149 (MQ=255)
        aaGGTTGGCGAAGGCCGTTGTGGCACGGTTACCAAACGCTTTCAGCAAGCCTTCTTCGTCCTCTTCACTGGCGAAACCGAAGATAAATGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGTGCGTGCCGTCCCa                                                                                                                                           <  1:916738/148‑1 (MQ=255)
          ggTTGGCGAAGGCCGTTGTGGCCCGGTTACCAAACGCATTCAGCAAGCCTTCTTCGGCCTCTTCACTGGCGAACCCGAAGATAAATGGGGCTGGTGAGCAGAAGGGAATCAATTAATAAAAATAATTGGACGGACCGCTCCGTACCagg                                                                                                                                         >  1:694221/1‑147 (MQ=255)
           gTTGGCGAAGGCCGTTGTGGCCCGGTTACCAAACGCATTCAGCAAGCCTTCTTCGGCCTCTTCACTGGCGAAACCGAAGATAAATGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCAttt                                                                                                                                        >  2:164138/1‑149 (MQ=255)
              ggCGAAGGCCGTTGTGGCCCGGTTACCAAACGCATTCAGCAAGCCTTCTTCGGCCTCTTCACTGGCGAAACCGAAGATAAATGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGTGCGTGCCGTCCCAtttttt                                                                                                                                     <  2:710643/149‑4 (MQ=255)
                cGAAGGCCGTTGTGGCCCGGTTACCAAACGCATTCAGCAAGCCTTCTTCGGCCTCTTCACTGGCGAAACCGAAGATAAATGGGGCTGGTTAGAACAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACgag                                                                                                                                   <  1:73660/149‑1 (MQ=255)
                       cGTTGTGGCCCGGTTACCAAACGCATTCAGCAAGCCTTCTTCGGCCTCTTCACTGGCGAAAGCGAAGTTAAATGTGGCTGGTTAGATCAAGTTAATCAATAAATACTAAAAATGGGACGGCACGCACCGTCGCATTTACGAgccagaca                                                                                                                            >  1:598669/1‑149 (MQ=255)
                          tgtgGCCCGGTTACCAAACGCATTCAGCAAGCCTTCTTCGGCCTCTTCACTGGCGAAACCGAAGATAAATGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGACACTg                                                                                                                         <  2:140998/149‑1 (MQ=255)
                           gtgGCCCGGTTACCAAACGCATTCAGCAAGCCTTCTTCGGCCTCTTCACTGGCGAAACCGAAGATAAATGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGACACTgg                                                                                                                        <  1:253774/149‑1 (MQ=255)
                           gtgGCCCGGTTAACAAACGCATTCAGCAAGCCTTCTTCGGCCGCTTCACTGGCGTATCCGAAGATAATTGGGGCTGGTTAGATCAAGTTAATCAATAATTACAAAAATTGGGAGGGCAGGCACCGTCCCATTTACGAGACAGACACTgg                                                                                                                        <  2:900157/149‑1 (MQ=255)
                              gCCCGGTTACCAAACGCATTCAGCAAGCCTTCTTCGGCCTCTTCACTGGCGAAACCGAAGATAAATGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCAGTTACGAGCCAGCCACTGGGAg                                                                                                                     >  1:423689/1‑149 (MQ=255)
                                ccGGTTACCAACCGCATTCAGCAAGCCTTCTTCGGCCTCTTCCCTGGCGAAACCGAAGATAAATGGGGCGGGTTAGATCAAGTTAAGCAATAAATACAAAAAATGGGACGGGACGACCCGTCCCAGTTACGAGCCTGACACTGGGAgga                                                                                                                   >  2:679327/1‑147 (MQ=255)
                                 cGGTTACCAAACGCATTCAGCAAGCCTTCTTCGGCCTCTTCACTGGCGAAACCGAAGATAAATGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGACACTGGGAGtaa                                                                                                                  <  1:258802/149‑1 (MQ=255)
                                      aCCAAACGCATTCAGCAAGCCTTCTTCGGCCTCTTCACTGGCGAAACCGAAGATAAATGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGACACTGGGAGtaaataaa                                                                                                             <  2:156319/149‑1 (MQ=255)
                                       ccAAACGCATTCAGCAAGCCTTCTTCTGCCTCTTCACTGGCGAAACCGAAGATAAATGGGGCTGGTTAGATCAAGTTAATCAATAAAAACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGACACTGGGAGTAAATAAAg                                                                                                            <  2:937235/149‑1 (MQ=255)
                                        cAAACGCATTCAGCAAGCCTTCTTCGGCCTCTTCACTGGCGAAACCGAAGATAAATGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGACACTGGGAGTAAATAAAg                                                                                                            >  1:643692/1‑148 (MQ=255)
                                        cAAACGCATTCAGCAAGCCTTCTTCGGCCTCTTCACTGGCGAAACCGAAGATAAATGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGACACTGGGAGTAAATAAAg                                                                                                            <  2:643692/148‑1 (MQ=255)
                                        cAAACGCATTCAGCAAGCCTTCTTCGGCCTCTTCACTGGCGAAACCGAAGATAAATGGGGCTGGTAAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGACACTGGGAGTAAATAAAGt                                                                                                           >  1:82599/1‑149 (MQ=255)
                                         aaaCGCATTCAGCAAGCCTTCTTCGGCCTCTTCACTGGCGAAACCGAAGATAAATGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGACACTGGGAGTAAATAAAGTa                                                                                                          <  1:137478/149‑1 (MQ=255)
                                                ttCAGCAAGCCTTCTTCGGCCTCTTCACTGGCGAAACCGAAGATAAATGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGACACTGGGAGTAAATAAAGTATGCCTaa                                                                                                   >  1:520494/1‑149 (MQ=255)
                                                    ggaaGCCTTCTTCGGCCTCTTCTCTGGCGAAACCGAAGATAAATGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGACACTGTGAGTAAATAAAGTATGCCTAAGTAc                                                                                               <  2:356232/147‑1 (MQ=255)
                                                    gCAAGCCTTCTTCGGCCTCTTCACTGGCGAACCCGAAGATAAATGGGGCTGGTTAGATCAAGTTACTCAATAAATACAAAAAATGGGACGGCACGCACCGACCCATTTACGAGAAAGACACTGGGAGTAAATAAAGTATGACTAAGTAc                                                                                               >  2:569866/1‑149 (MQ=255)
                                                     ctatcattcttcTGCCTATTCACTGGCGAAACCGAAGATAAATGGGGCTGGTTAGATAAAGTTAATCAATAAATACAAAAAATGGTACGGCACGCACCGTCCCATTTACGAGACAGACACTGGGAGTAAATAAAGTATGCCTAAGTAcg                                                                                              <  2:949848/143‑2 (MQ=255)
                                                            tcttcGGCCTCTTCACTGGCGAAACCGAAGATAAATGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGACACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGc                                                                                       <  2:711253/149‑1 (MQ=255)
                                                                  gCCTCTTCACTGGCGAAACCGAAGATAAATGTGGCTGGTTAGAACAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGACACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGccaccac                                                                                 <  1:872349/149‑1 (MQ=255)
                                                                  gCCTCTTCACTGGCGAAACCGAAGATAAATGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGACACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCTccaccac                                                                                 >  2:40516/1‑149 (MQ=255)
                                                                   ccTCTTCACTGGCGAAACCGAAGATAAATGGGGCTGGTTAGATCAACTTAAACAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGGCACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTACTccaccacc                                                                                >  1:323217/1‑149 (MQ=255)
                                                                    ctctTCACTGGCGAAACCGATGAAAAATGGGGCTGGTTAGATCAAGTTAATCAATTAATACAAAAAATGGGACGTAACGAACAGTCCAATTTACGAGACAGACACTGGGAGTTAATAAAGTATGCCTAAGTAGCGTTCCGccaccacca                                                                               >  2:730953/1‑149 (MQ=255)
                                                                           cTGGCGAAACCGAAGATAAATGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGACACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGCCACCACCACTCATgg                                                                        <  2:498770/149‑1 (MQ=255)
                                                                           cTGGCGAAACCGAAGATAAATGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGACACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGCCACCACCACTCATgg                                                                        >  2:444042/1‑149 (MQ=255)
                                                                                 aaaCCGAATATAATTGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATTGGACGGCACGCACCGTCCCATTTACGAGACAGACACTGGGATTAAATAAAGTATGCCTAAGTTCCGTTCCGCCACCACCACTCATGGGCGTaa                                                                  <  2:211277/149‑1 (MQ=255)
                                                                                      gAAGATAAATGGGGCTGGTTAGATCAAGATAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGACACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGCCACCACCACTCATGGTCGTAATATgg                                                             <  1:340694/149‑1 (MQ=255)
                                                                                      gAAGATAAATGGGGCTGGTTAGAACAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTAACATTTACGAGACAGACACTGGGAGTAAATAAAGTATGCCTAAGAACCGTTCCGCCACCACCACTAAAGGTCGTAATATgg                                                             >  1:43204/1‑149 (MQ=255)
                                                                                                  ggCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGACACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGCCACCACCACTCATGGTCGTAATATGGCGGGTGATCGTg                                                 >  2:72583/1‑149 (MQ=255)
                                                                                                      ggTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGACACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGCCACCACCACTCATGGTCGTAATATGGCGGGTGCTCGTGCGCt                                             >  2:436126/1‑149 (MQ=255)
                                                                                                        ttAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGCCACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGCCACCACCACTCATGGTCTTAATATGGCGGTTGCTCCTGCACttt                                           >  2:198333/1‑147 (MQ=255)
                                                                                                         tAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGACACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGCCACCACCACTCATGGTCGTAATATGGCGGGTGCTCGTGCGCtgtg                                          <  1:930207/149‑1 (MQ=255)
                                                                                                         tAGATCAAGCTAATCAATAAATACAAAAACTGGGACGGCACGCACCGTCCCATTTACGAGACAGACACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGCCACCACCACTCATGGTCGTAATATGGCGGGTGCTCGTGCGCtgtg                                          <  2:4044/149‑1 (MQ=255)
                                                                                                            aTCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACACACACTGGGAGTAAATAAAGTATGCCTAAGTACCGTACCGCCACCACCACTCATGGTCGTAATATGGCGGGTGCTCGTGCGCTGTGgcg                                       >  2:140393/1‑149 (MQ=255)
                                                                                                             tCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGACACTGGGAGTAAATAAAGTATGCCTAAGTTCCGTTCCGCCACCACCACTCATGGTCGTAATATGGCGGGTGCTCGTGCGCTGTGgcgc                                      <  2:347620/149‑1 (MQ=255)
                                                                                                               aaGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGACACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGCCACCACCACTCATGGTCGTAATATGGCGGGTGCTCGTGCGCTGTGgcgcgc                                    >  1:913364/1‑149 (MQ=255)
                                                                                                                aGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGACACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGCCACCACCACTCATGGTCGTAATATGGCGGGTGCTCGTGCGCTGTGGCGCGcc                                   >  1:6623/1‑149 (MQ=255)
                                                                                                                 gTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGACACTGGGAGTAAATAAAGTAAGCCTAAGTACCGTACCGCCACCACCCCGCATGGGCGAAATATGGCGGCGGATCTTGCGCTGTGGCGCGGCa                                  >  2:421997/1‑149 (MQ=255)
                                                                                                                    aaTCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGCCACTGGGAGTAAATAAAGTATGCCTCAGTACCGTTCCGCCACCCCCCCTCATGGTCGTAATAGGGCGGGTGCTCGTGCGCTGTGGCGCGCCCCCg                               >  2:271719/1‑149 (MQ=255)
                                                                                                                    aaTCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGACACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGCCACCACCACTCATGGTCGTAATATGGCGGGTGCTCGTGCGCTGTGGCGCGCCACCg                               >  2:403789/1‑149 (MQ=255)
                                                                                                                      tCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGACACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGCCACCACCACTCATGGTCGTAAGATGGCGGGTGCTCGTGCGCTGTGGCGCGCCACCGGa                             >  2:337372/1‑149 (MQ=255)
                                                                                                                       cAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGACACTGGGAGTAATTAAAGTATGCCTAAGTACCGTTCCGCCCCCACCACTCATGGTCGGAATATGGCGGGTGCTCGTGCGCTGTGGCGCGCCACCGGaa                            >  2:291169/1‑149 (MQ=255)
                                                                                                                       cAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGACACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGCCACCACCACTCATGGTCGTAATATGGCGGGTGCTCGTGCGCTGTGGCGCGCCACCGGaa                            <  1:749263/149‑1 (MQ=255)
                                                                                                                       cAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGACACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGCCACCACCACTCATGGTCGTAATATGGCGGGTGCTCGTGCGCTGTGGCGCGCCACCGGaa                            <  1:805093/149‑1 (MQ=255)
                                                                                                                        aataaataCAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGACACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGCCACCACCACTCATGGTCGTAATATGGCGGGTGCTCGTGCGCTGTGGCGCGCCACCGGAAt                           <  2:392865/149‑1 (MQ=255)
                                                                                                                         ataaataCAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGACACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGCCACCAACACTCATGTTCGTAATATGGCTGGTGATCGTGCGCTGTGGCGCGCCACCGGAATg                          >  2:995742/1‑149 (MQ=255)
                                                                                                                           aaataCAAAAAATGGGACGGCCCGCACCGTCCCATTTACGAGACAGACACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGCCACCACCACTCATGGTCGTAATATGGCGGGTGCTCGTGCGCTGTGGCGCGCCACCGGAATGAc                        <  2:114310/149‑1 (MQ=255)
                                                                                                                            aataCAAAAAATGGGACCGCACGGACCGGCCCATTTACGAGACAGACACTGGGAGTAACTAAAGTATGCCTAAGTACCGTTCCGCCACCACCACTCATGGTCGTAATATGGCGGGTGCTCGTGCGCTGTGGCGCGCCACCGGAATGAcc                       <  2:1029245/149‑1 (MQ=255)
                                                                                                                             ataCAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGACACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGCCACCACCACTCATGGTCGTAATATGGCGGGTGCTCGTGCGCTGTGGCGCGCCACCGGAATGACCg                      >  1:122440/1‑149 (MQ=255)
                                                                                                                               aCAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGACACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGCCACCACCACTCATGGGCGTATTATGGCGGGTGCTCGTGCGCTGTGGCGCGCCACCGGAATGACCGAc                    >  1:154357/1‑149 (MQ=255)
                                                                                                                                 aaaaaaTGGTACGGGACGCCACGTCCCATTTACGAGACAGGCACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCTCCACCACCACTCATGGTCGTAATATGGCGGGTGCTCGTGCGCTGTGGCGCGCCACCGGAATGACCTACGc                  <  2:323217/149‑1 (MQ=255)
                                                                                                                                 aaaaaaTGGGACGGCACGCACCGTCCCATTTACGAGACAGACACTGGGAGTAAATAAAGTATGCGTAAGTACCGTTCCGCCACCACCACTCATGGTCGTAATATGGCGGGTGCTCGTGCGCTGTGGCGCGCCTCCGGAATGAGCGCCgg                  >  2:421843/1‑148 (MQ=255)
                                                                                                                                    aaaTGGGACGGCACGCACCGTCCCATTTACGAGACAGACACTGGGAGTAAATAAAGTATGCCTAAGTCCCGTTCCGCCACCACCACTCATGGTCGTAATATGGCGGGTGCTCGTGCGCTGTGGCGCGCCCCCGGAATGACCGACGCCGa               >  1:97957/1‑149 (MQ=255)
                                                                                                                                      aTGGGACGGTGCGTGCCGTCCCATTTACGAGACAGACACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGCCACCACCACTCATGGTCGTAATATGGCGGGTGCTCGTGCGCTGTGGCGCGCCACCGGAATGTCCGACGCCGAtt             >  2:157121/1‑149 (MQ=255)
                                                                                                                                      aTGGGACGGTGCGTGCCGTCCCATTTACGAGACAGACACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGCCACCACCACTCATGGTCGTAATATGGCGGGTGCTCGTGCGCTGTGGCGCGCCACCGGAATGACCGACGCCGAtt             >  2:645072/1‑149 (MQ=255)
                                                                                                                                        gggACGGCACGCACCGTCCCATTTACGAGACAGACACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGCCACCACCACTCATGGTCGTAATATGGCGGGTGCTCGTGCGCTGTGGCGCGCCACCGGAATGACCGACGCCGATTTc           <  2:82599/149‑1 (MQ=255)
                                                                                                                                          gACGGCACGCACCGTCCCATTTACGAGACAGACACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGCCACCACCACTCATGGTCGTAATATGGCGGGTGCTCGTGCGCTGTGGCGCGCCACCGGAATGACCGACGCCGATTTCgg         <  1:386326/149‑1 (MQ=255)
                                                                                                                                                acgcacCGTCCCATTTACGAGACAGACACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGCCCCCACCACTCATGGTAGTAAGATTGCGGTTGCTCGTGCGCTGTGGCGGGCCACCGGAATGACCGACGCCGATTTCGGTAAGcc   >  2:710834/1‑149 (MQ=255)
                                                                                                                                                 cgcaaCGTACCATTTACGAGACAGACACTGGGAGTAAATAAAGTATGCCTAAGTACCGATCCGCCACCACCACTCATGGTCGTAATATGGCGGGTGCTCGTGCGCTGTGGCGCGCCACCGGAATGACCGACGCCGATTTCGGTAAGCCg  <  2:423689/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
ACGGTATTCAGGTTGGCGAAGGCCGTTGTGGCCCGGTTACCAAACGCATTCAGCAAGCCTTCTTCGGCCTCTTCACTGGCGAAACCGAAGATAAATGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGACACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGCCACCACCACTCATGGTCGTAATATGGCGGGTGCTCGTGCGCTGTGGCGCGCCACCGGAATGACCGACGCCGATTTCGGTAAGCCG  >  NC_000913/3953289‑3953582

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: