Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 373,332 C→G 6.4% P138A (CCA→GCA)  mhpF → acetaldehyde‑CoA dehydrogenase II, NAD‑binding

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913373,3320CG 6.4% 177.5 / 4.9 74P138A (CCA→GCA) mhpFacetaldehyde‑CoA dehydrogenase II, NAD‑binding
Reads supporting (aligned to +/- strand):  ref base C (41/27);  new base G (3/2);  total (44/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.20e-01

CGCTTTACGCGAAGCGAAACCGGATATTCGCTTAATTGACCTGACGCCTGCTGCCATCGGCCCTTACTGCGTGCCGGTGGTTAACCTCGAGGCGAACGTCGATCAACTGAACGTCAACATGGTCACCTGCGGCGGCCAGGCCACCATTCCAATGGTGGCGGCAGTTTCACGCGTGGCGCGTGTTCATTACGCCGAAATTATCGCTTCTATCGCCAGTAAATCTGCCGGACCTGGCACGCGTGCCAATATCGATGAATTTACGGAAACCACTTCCCGAGCCATTGAAGTGGTGGGCG  >  NC_000913/373184‑373479
                                                                                                                                                    |                                                                                                                                                   
cGCTTTACGCGAAGCGAAACCGGATATTCGCTTAATTGACCAGACGCCTGCTGCCATCGGCCCTTACTGCGTGCCGGTGGTTAACCTCGAGGCGAACGTCGATCAACTGAACGGCAACATGGTCACCTGCGGCGGCCAGGCCACCATTc                                                                                                                                                     <  2:811909/149‑1 (MQ=255)
     tACGCGAAGCGAAACCGGATATTCGCTTAATTGACCTGACGCCTGCTGCCATCGGCCCTTACTGCGTGCCGGTGGTTAACCTCGAGGCGAACGTCGATCAACTGAACGTCAACATGGTCACCTGCGGCGGCCAGGCCACCATTCCAAtg                                                                                                                                                <  2:158607/149‑1 (MQ=255)
        gcgaagcgaaACCGGATATTCGCTTAATTGACCTGACGCCTGCTGCCATCGGCCCTTACTGCGTGCCGGTGGTTAACCTCGCGGCGAACGTCGATCAACTGAACGTCAACATGGTCACCTGCGGCGGCCAGGCCACCATTCCAAgggag                                                                                                                                             >  2:223356/1‑147 (MQ=255)
        gcgaagcgaaACCGGATATTCGCTTAATTGACCTGACGCCTGCTGCCATCGGCCCTTACTGCGTGCCGGTGGTTAACCTCGAGGCGAACGTCGATCAACTGAGCGTCAACATGGTCACCTGCGGCGGCCAGGCCAGCATTCCAAtggtg                                                                                                                                             >  1:205706/1‑149 (MQ=255)
        gcgaagcgaaACCGGATATTCGCTTAATTGACCTGACGCCTGCTGCCATCGGCCCTTACTGCGTGCCGGTGGTTAACCTCGAGGCGAACGTCGATCAACTGAACGTCAACATGGTCACCTGCGGCGGCCCGGCCACCATTCCAAtggtg                                                                                                                                             >  1:318332/1‑149 (MQ=255)
          gatgcgacACCGGATATTCGCTTAATTGACCTGACGCAAGCTGCCAACGGCCCTTAGTGCTTCCCGGTGGTTAACCTCGAGGCGACCGTCGAAACACTGAACGTCAACATGGTCACCTGCGGCGGCCAGGCCCCCAGTCCCATGGTggc                                                                                                                                           >  2:319459/4‑149 (MQ=255)
           aagcgaaACCGGATATTCGCTTAATTGACCTGACGCCTTCTGCCATATGACCTTACTGCGTGCCGGTCGTTAACCTCGATGAGAACTTCGATCAACTGAACGTCAACATGGTCACCTGCGGCGGCCAGGCCACCATTCCAATTGTggcg                                                                                                                                          >  2:719282/1‑149 (MQ=255)
            agcgaaACCGGATATTGGCTTAATTGACCTGACGCCTGCTGCCATCGGCCCTTACTGCGTGCCGGTTGTTAACCTCGAGGCGAACGTCGATCAACTGAACTTCAACATGGTCACCTGCGGCGGCCAGGCAACCATTGGAATGGTggcct                                                                                                                                         <  2:621232/149‑3 (MQ=255)
             gcgaaACCGGATATTCGCTTAATTGACCTGACGCCTGCTGCCATCGGCCCTTACTGCGTGCCGGTGGTTAACCTCGAGGCGAACGTCGATCAACTGAACGTCAACATGGTCACCTGCGGCGGCCAGGCCACCATTGGAATGGTggcctg                                                                                                                                        <  1:136309/149‑4 (MQ=255)
               gaaACCGGATATTCGCTTAATTGACCTGACGCCTGCTGCCATCGGCCCTTACTGCGTGCCGGTGGTTAACCTCGAGGCGAACGTCGATCAACTGAACGTCAACATGGTCACCTGCGGCGGCCAGGCCACCATTGCAAGGGTGGCGGCAg                                                                                                                                      >  2:695949/1‑149 (MQ=255)
                aaaCCGGATATTCGCTTAATTGACCTGACGCCTGCTGCCATCGGCCCTTACTGCGTGCCGGTGGTTAACCTCGAGGCGAACGTCGATCAACTGAACGTCAACATGGTCACCTGCGGCGGCCAGGCCACCATTCCAATGGTGGCGGCAGt                                                                                                                                     >  1:154765/1‑149 (MQ=255)
                  aCCGGATATTCGCTTAATTGACCTGACGCCTGCTGCCATCGGCCCTTACTGCGTGCCGGTGGTTAACCTCGAGGCGAACGTCGATCAACTGAACGTCAACATGGTCACCTGCGGCGGCCAGGCCACCATTCCAATGGTGGCGGCAGttt                                                                                                                                   <  1:832889/149‑1 (MQ=255)
                   ccGGATATTCGCTTAATTGACCTGACGCCTGCTGCCATCGGCCCTTACTGCGTGCCGGTGGTTAACCTCGAGGCGAACGTCGATCAACTGAACGTCAACATGGTCACCTGCGGCGGCCAGGCCACCATTCCAAAGGTGGCGGCAGctta                                                                                                                                  >  1:969353/1‑148 (MQ=255)
                    cGGATATTCGCTTAATTGACCTGACGCCTGCTGCCATCGGCCCTTACTGCGTGCCGGTGGTTAACCTCGAGGCGAACGTCGATCAACTGAACGTCAACATGGTCACCTGCGGCGGCCAGGCCACCATTCCAATGGTGGCGGCAGTTTCa                                                                                                                                 <  2:402526/149‑1 (MQ=255)
                     ggATATTCGCTTAATTGACCTGACGCCTGCTGCCATCGGCCCTTACTGCGTGCCGGTGGTTAACCTCGAGGCGAACGTCGATCAACTGAACGTCAACATGGTCACCTGCGGCGGCCAGGCCACCATTCCAATGGTGGCGGCAGTTTCAc                                                                                                                                <  2:355909/149‑1 (MQ=255)
                            cGCTTAATTGACCTGACGCCTGCTGCCATCGGCCCTTACTGCGTGCCGGTGGTTAACCTCGAGGCGAACGTCGATCAACTGAACGTCAACATGGTCACCTGCGGCGGCCAGGCCACCATTCCAATGGTGGCGGCAGTTTCACGCGTGgc                                                                                                                         <  2:42758/149‑1 (MQ=255)
                              cTTAATTGACCTGACGCCTGCTGCCATCGGCCCTTACTGCGGGCCGGTGGTTAACCTCGAGGCGAACGTCGATCAACTGAACGTCAACATGGTCACCTGCGGCGGCCAGGCCACCATTCCAATGGTGGCGGCAGTTTCACGCGTGgcgc                                                                                                                       <  1:821008/149‑1 (MQ=255)
                               ttAATTGACCTGACGCCTGCTGCCATCGGCCCTTACTGCGTGCCGGTGGTTAACCTCGAGGCGAACGTCGATCAACTGAACGTCACCATGGTCACCTGCGGCGGCCAGGCCACCATTCCAATGGTGGCGGCAGTTTCACGCGTGGcgcg                                                                                                                      >  1:271068/1‑149 (MQ=255)
                                         tGACGCCTGCTGCCATCGGCCCTTACTGCGTGCCGGTGGTTAACCTCGAGGCGAACGTCGATCAACTGAACGTCAACATGGTCACCTGCGGCGGCCAGGCCACCATTCCAATGGTGGCGGCAGTTTCACGCGTGGCGCGTGTTCATTAc                                                                                                            <  1:869588/149‑1 (MQ=255)
                                          gACGCCTGCTGCCATCGGCCCTTACTGCGTGCCGGTGGTTAACCTCGAGGCGAACGTCGATCAACTGAACGTCAACATGGTCACCTGCGGCGGCCCGGCCACCATTCCAATGGTGGCGGCAGTTTCACGCGTGGCGCGTGTTCATTACg                                                                                                           >  1:528775/1‑149 (MQ=255)
                                             gCCTGCTGCCATCGGCCCTTACTGCGTGCCGGTGGTTAACCTCGAGGCGAACGTCGAGCAACTGAACGTCAACATGGTCACCTGCGGCGGCCAGGCCCCCATTCCAATGGTGGCGGCAGTGTCACGCGTGGCGCGTGTGCATTACGCCg                                                                                                        >  1:884279/1‑149 (MQ=255)
                                                tgctgcCATCGGCCCTTACTGCGTGCCGGTGGTTAACCTCGAGGCGAACGTCGATCAACTGAACGGCAACATGGTCACCTGCGGCGGCCAGGCCACCATTCCAATGGTGGCGGCAGTTTCACGCGTGGCGCGTGTTCATTACGCCGaaa                                                                                                     <  2:352022/149‑1 (MQ=255)
                                                tgctgcCACCGGCCCTTACTGCGTGCCGGTGGTTAACCTCGAGGCGAACGTCGATCAACTGAACGTCAACATGGTCACCTGCGGCGGCCAGGCCACCATTCCAATGGTGGCGGCAGTTTCACGCGTGGCGCGTGTTCATTACGCCGaaa                                                                                                     >  2:581963/1‑149 (MQ=255)
                                                 gctgcCATCGGCCCTTACTGCGTGCCGGTGGTTAACCTCGAGGCGAACGGCGATCAACTGAACGTCAACAAGGTCACCTGCGGCGGCCAGGCCACCATTCCAATGGTGGCGGCAGATTCACGCGTGGCGCGTGTTCATTACGCCGAAAt                                                                                                    >  2:408359/1‑149 (MQ=255)
                                                    gcCATCGGCCCTTACTGCGTGCCGGTGGTTAACCTCGAGGCGAACGTCGATCAACTGAACGTCAACATGGTCACCTGCGGCGGCCAGGCCACCATTCCAATGCTGGCGGCAGTTTCACGCGTGGCGCGTGTTCATTACGCCGAAATTAt                                                                                                 >  2:159202/1‑149 (MQ=255)
                                                         cGGCCCTTACTGCGTGCCGGTGGTTAACCTCGAGGCGAACGTCGATCAACTGAACGTCAACATGGTCACCTGCGGCGGCCAGGCCACCATTCCAATGGTGGCGGCAGTTTCACGCGTGGCGCGTGTTCATTACGCCGAAATTATCGCtt                                                                                            <  1:716131/149‑1 (MQ=255)
                                                              cTTACTGCGTGCCGGTGGTTAACCTCGAGGCGAACGTCGATCAACTGAACGGCAACATGGGCACCTGCGGCGGCCAGGCCACCATTCCAATGGTGGCGGCAGTTTCACGCGTGGCGCGTGTTCATTACGCCGAAATTATCGCTTCTATc                                                                                       <  1:350147/149‑1 (MQ=255)
                                                                 aCTGCGTGCCGGTGGTTCACCTCGAGGCGAACGTCGATCAACTGAACGGCACCATGGTCACCTGCGGCGGCCAGGACACCATTCCAATGGTGGCGGCAGTTTCACGCGTGGCGCGTGTTCATTACGCCGAAATTATCGCTTCTATCGcc                                                                                    <  2:741340/149‑1 (MQ=255)
                                                                   tGCGTGCCGGTGGTTAACCTCGAGGCGAACGTCGATCAACTGAACGTCAACATGGTCACCTGCGGCGGCCAGGCCACCATTCCAATGGTTGCGGCAGTTTCACGCGTGGCGCGTGTTCATTACGCCGAAATTATCGCTTCTATCGCCAg                                                                                  >  1:188149/1‑149 (MQ=255)
                                                                        gCCGCTGGTTAACCTCGAGGCGAACGTCGATCAACTGAACGTCAACATGGTCACCGGCGGCGGCCAGGCCACCATTCCAATGGGGGCGGCAGTTTCACGCGTGGCGCGTGTTCATTACGCCGAAATTATCGCTGCTATCGCCAGTAAAt                                                                             <  2:929200/149‑1 (MQ=255)
                                                                          cGGTGGTTAACCTCGAGGCGAACGTCGATCAACTGAACGTCAACATGGTCACCTGCGGCGGCCAGGCCCCCATTCCAATGGTGGCGGCAGTTTCCCGCGTGGCGCGGGTTCATTACGCCGAAATTATCGCTTCTATCGCCAGTAAATCt                                                                           >  1:497156/1‑149 (MQ=255)
                                                                              ggtTAACCTCGAGGCGAACGTCGATCAACTGAACGTCAACATGGTCACCTGCGGCGGCCAGGCCACCATTCCAATGGTGGCGGCAGTTTCACGCGTGGCGCGTGTTCATTACGCCGAAATTATCGCTTCTATCGCCAGTAAATCTGCCg                                                                       <  2:373909/149‑1 (MQ=255)
                                                                                ttAACCTCGAGGCGAACGTCGATCAACTGAACGTCAACATGGTCACCTGCGGCGGCCAGGCCACCATTCCAATGGTGGCGGCAGTTTCACGCGTGGCGCGTGTTCATTACGCCGAAATTATCGCTTCTATCGCCAGTAAATCTGCCGGa                                                                     >  1:1021080/1‑149 (MQ=255)
                                                                                 tacccTCGAGGCGAACGTCGCTCAACTGAACGTCACCATGCGCCCCTGCGGCGGCCAGGCCACCATACCAATGGTGGCGGCAGCTGCACGCGTGGCGCGTGTTCATTACGCCGAAATTATCGCTTCTATCGCCAGTAACTCTGCCGGAc                                                                    <  2:896788/146‑1 (MQ=255)
                                                                                 tAACCTCGAGGCGAACGTCGATAAACTGAACGTCAACATGGTCACCTGCGGCGGCCAGGCCACCATTCCAATGGTGGCGGCAGTTTCACGCGTGGCGCGTGTTCATTAGGCCGAAATTATCGCTTCTATCGCCAGTAAATCTGCCggcc                                                                    >  2:971411/1‑147 (MQ=255)
                                                                                  aaCCTCGAGGCGAACGTCGATCAACTGAACGTCAACATGGTCACCTGCGGCGGCCAGGCCACCATACCAATGGTGGCGGCAGTTTCACGCGTGGCGCGTGTTCATTACGCCGAAATTATCGCTTCTATCGCCAGTAAATCTGCCGGAcc                                                                   <  1:434432/149‑1 (MQ=255)
                                                                                    ccTCGAGGCGAACGTCGATCAACTGAACGTCAACATGGTCACCTGCGGCGGCCAGGCCACCATTCCAATGGTGGCGGCAGTTTCCCGCGTGGCGCGTGTTCATTACGCCGAAATTATCGCTTCTATCGCCAGTAAATCTGCCGGACCTg                                                                 >  2:1015648/1‑149 (MQ=255)
                                                                                    ccTCGAGGCGAACGTCGATCAACTGAACGTCAACATGGTCACCTGCGGCGGCCAGGCCACCATTCCAATGGTGGCGGCAGTTTCACGCGTGGCGCGTGTTCATTCCGCCGAATTTATCGCTTCTATCGCCAGTAAATCTGCCGGACCTg                                                                 >  2:851702/1‑149 (MQ=255)
                                                                                      tCGAGGCGAACGTCGATCAACTTAACGTCAACATGGTCACCTGCGGCGGCCAGGCCACCATTCCAATTGTCGCGGCAGTTTCAAGCGTGACGCGTCTTCATTAAGCCGAAATTATCGCTTCTATCGCCAGTAAATCTGCCGGACCTGGc                                                               >  2:77463/1‑149 (MQ=255)
                                                                                      tCGAGGCGAACGTCGATCAACTGAACGTCAACATGGTCACCTGCGGCGGCCAGGCCACCATTCCAATGGTGGCGGCAGTTTCACGCGTGGCGCGTGTTCATTACGCCGAAATTATCGCTTCTATCGCCAGTAAATCTGCCGGACCTGGc                                                               >  2:19729/1‑149 (MQ=255)
                                                                                        gAGGCGAACGTCGATCAACTGAACGTCAACATGGTCACCTGCGGCGGCCAGGCCACCATTCCAATGGTGGCGGCAGTTTCACGCGTGGCGCGTGTTCATTACGCCGAAATTATCGCTTCTATCGCCAGTAAATCTGCCGGACCTGGCAc                                                             <  1:344969/149‑1 (MQ=255)
                                                                                            cGAACGTCGATCAACTGAACGTCAACATGGTCACCTGCGGCGGCCAGGCCACCATTCCAATGGTGGCGGCAGTTTCACGCGTGGCGCGTGTTCATTACGCCGAAATTATCGCTTCTATCGCCAGTAAATCTGCCGGACCTGGCACGCGt                                                         <  1:290078/149‑1 (MQ=255)
                                                                                             gAACGTCGCTCAACTGAACGTCAACATGGTCACCTGCGGCGGCCAGGCCACCATTCCAATGGTGGCGGCAGTTTCACGCGTGGCGCGTGTTCATTACGCCGAAATTATCGCTTCTATCGCCAGTAAATCTGCCGGACCTGGCACGCGTg                                                        >  2:34444/1‑149 (MQ=255)
                                                                                             gAACGTCGATCAACTGAACGTCAACATGGTCACCTGCGGCGGCCAGGCCACCATTCCAATGGTGGCGGCAGTTTCACGCGTGGCGCGTGTTCATTACGCCGAAATTATCGCTTCTATCGCCAGTAAATCTGCCGGACCTGGCACGCGTg                                                        >  1:463750/1‑149 (MQ=255)
                                                                                                    gATCAACTGAACGTCAACATGGTCACCTGCGGCGGCCAGGCCACCATTCCAATGGTGGCGGCAGTTTCACGCGTGGCGCGTGTTCATTACGCCGAAATTATCGCTTCTATCGCCAGTAAATCTGCCGGACCTGGCACGCGTGCCaggtc                                                 <  1:581963/149‑5 (MQ=255)
                                                                                                     ctCAACTGAACGTCAACAAGGTCCCCAGCGGAGGCCAGGCCCCCATTCCAATGGTGGCGGCAGTTTCACGCGTTGCGCGTGGTCCTTACCCCGAAAATATAGCGTCTATCGCCAGTAAAAATGCCGGACCTGGCCCGCGTGCCAATATc                                                >  2:225349/2‑149 (MQ=255)
                                                                                                     agcatcTGCACGTCAACATGGTCACCTGCGGCGGCCAGGCCACCATTCCATTGGTGGCGGCAGTTTCACGCGTGGCGCGTGTTCAGTACGCCGAAATGATCGCTTCTCTCGCGAGGAAATCGGCCGGACCGTGCACGCGTGCCAATATc                                                >  1:598885/6‑149 (MQ=255)
                                                                                                     aacAACTGAACGTCAACATGGTCACCTGCGGCGGCCAGGCCACCATTCCAATGGTGGCGGCAGTTTAACGCGTGGCGCGTGTTCATTACGCCGAAATTATCGCTTCTATCGCCAGTAAATCTGACGGACCTGGCACGCGTGCCAATATc                                                >  2:24081/3‑149 (MQ=255)
                                                                                                     aTCAACTGAACGTCACCCTGGTCACCTGCGGCGGCCAGGCCACCATTCCAATGGTGGCGGCAGTTTCCCGCGTGGCGCGTGTTCATTACGCCGAAATTATCGCTTCTATCGCCAGTATATCTGCCGGACCTGGAACGCGTGCCAATATc                                                >  1:598883/1‑149 (MQ=255)
                                                                                                     aTCAACTGAACGTCAACATGGACACCTGCGGCGGCCAGGCCACCATTCAAATTTTGTCGGCAGTTTCACGCGTGGCGCGTGATCATTACGCCGAAATTATCGCTTCTATCGCCAGTAAATCTGCCGGACCTGGCACGCGAGCCAATATc                                                >  1:740808/1‑149 (MQ=255)
                                                                                                      tCAACTGAACGTCAACATGGTCACCTGCGGCGGCCAGGCCACCATTCCAATGGTGGCGGCACTTTCACGCGTGGCGCGTGTTCATTACGCCGAAATTATCGCTTCTATCGCAAGTAAATCAGCCGGACCTGGCACGCGTGCCAAAATCg                                               >  2:256750/1‑149 (MQ=255)
                                                                                                       cAACTTACCGTAACCATGGTCCCCTGCGGCGGACAGGCCACCAATACAATGCTGGCGGCAGTATCCCGCGTTGCGCGTGTTCATTCATCAGAAATTATCGCATCTATCGCCAGTAAATCTGCCGGACCTGGCACTCGTGCCAATATCGa                                              <  1:208339/149‑1 (MQ=255)
                                                                                                       cAACTGAACGTCACCATCGTCACCTGCGGCGGCCAGGCCACCATTCCAATGCTGGCGGCAGTTTCACGCGTGGCGCGTGGTCATTACGCCGAAATACTCGCTTCTATCGCCAGTAAATCTGCCGGACCTGGCACGCGTGCCAATATCGa                                              <  1:23442/149‑1 (MQ=255)
                                                                                                        aaCTGAACGTCATCATGGTCACCTGCGGCGGCCAGGCCACCATTCCAATGGTGGCGGCAGTTTCAGGCGTGGCGCGTGTTCATTACGCCGAAATTATCGCTTCTATCGCCAGTAAATCTGCCGGACCTGGCACGCGTGCCAATATCGAt                                             <  2:393058/149‑1 (MQ=255)
                                                                                                        aaCTGAACGTCAACATGGTCACCTGCGGCGGCCAGGCCACCATTCCAATGGTGGCGGCAGTTTCACGCGTGGCGCGTGTTCATTACGCCGAAATTCTCGCTTCTATCGCCAGTAAATCTGCCGGACCTGGCACGCGTGCCAATATCGAt                                             <  1:256278/149‑1 (MQ=255)
                                                                                                         aCTGAACGACAACATGGTCACCTGCGGCGGCCAGGCCACCATTCCAATGGTGGCGGCAGTTTCACGCGTGGCGCGTGTTCATTACGCCGAAATTATAGCTTCTATCGCCAGTAAATCTGACGGACCAGGCACGCGTGCCAAAATCGAtc                                            >  2:763746/1‑148 (MQ=255)
                                                                                                           tcaaCGTCAACATGGTCACCTGCGGCGGCCAGGCCACCATACCAATGGTGGCGGCACTTTCACGCGTGTGTCGAGTTCATTACTCTGAAATTAACGCTTCTATCGCCAGTAAATCTCACGGACCTGACACGCGTGCCAATATCGATGaa                                          >  2:511215/3‑149 (MQ=255)
                                                                                                                gTCAACATGGTCACCTGCGGCGGCCAGGCCACCATTCCAATGGTGGCGGCAGTTTCACGCGTGGCGCGTGTTCATTACGCCGAAATTATCGCTTCTATCGCCAGTAAATCTGCCGGACCTGGCACGCGTGCCAATATCGATGAATTTAc                                     >  1:461468/1‑149 (MQ=255)
                                                                                                                   aaCATGGTCACCTGCGGCGGCCAGGCCACCATTCAAATGGTGGCGGCAGTTTCACGCGTGGCGCGTGTTCATTACGCCGAAATTATCGCTTCTATCGCCAGTAAATCTGCCGGACCTGGCACGCGTGCCATTATCGATGAATTTACGGa                                  >  2:790257/1‑149 (MQ=255)
                                                                                                                        ggTCACCTGCGGCGGCCAGGCCACCATTCCAATGGTGGCGGCAGTTTCACGCGTGTCGCGTGTTCATTACGCCGAAATTATCGCTTCTATCGCCAGTAAATCTGCCGGACCAGGCACGCGTGCCAATATCGATGAATTTACGGAAAccc                             >  2:195363/1‑148 (MQ=255)
                                                                                                                            cgcTGCGGCGGCCAGGCCACCATTCCACTGGTGGCGGCAGTTTCACGCGTGGCGCGTGTTCATTACGCCGAAATTATCGATTCTATCGAAAGTAAATCTGCCGGACCTGGCACGCGTGCCAATATCGATGAATTTACGGAAACCACTTc                         <  2:146351/147‑1 (MQ=255)
                                                                                                                               tGCGGCGGCCAGGCCACCATTCCAATGGTGGCGGCAGTTTCACGCGTGGCGCGTGTTCATTACGCCGAAATTATCGCTTCTATCGCCAGTAATTCTGCCGGACCTGGCACGCGTGCCAATTTCGATGCATTTACGGAAACCACTTCCCg                      >  1:763931/1‑149 (MQ=255)
                                                                                                                               tGCGGCGGCCAGGCCACCATTCCAATGGTGGCGGCAGTTTCACGCGTGGCGCGTGTTCATTACGCCGAAATTATCGCTTCTATCGCCAGTAAATCTGCCGGACCTGGCACGCGTGCCAATATCGATGAATTTACGGAAACCACTTCCCg                      <  1:294798/149‑1 (MQ=255)
                                                                                                                                      gccaggccaCCATTCCAATGGTGGCGGCAGTTTCACGCGTGGCGCGTGTTCATTACGCCGAAATTATCGCTTCTATCGCCAGTAAATCAGCCGGACCTGGCACGCGTGCCAATATCGATGAATTTACGGAAACCACTTCCCGAGCCAtt               <  1:870785/149‑1 (MQ=255)
                                                                                                                                      gccaggccaCCATTCCAATGGTGGCGGCAGTTTCACGCGTGGCGCGTGTTCATTACGCCGAAATTATAGCTTCTATCGCCAGTAAATCTGCCGGACCTGGCACGCGTGCCAATATCGATGAATTTACGGAAACCACTTCCCGAGCCAtt               >  2:346876/1‑149 (MQ=255)
                                                                                                                                      gccaggcaaCCATTCCAATGGTTGCGGCAGTTTCACGCGTGGCGCGTGTTCATTACTCCGAAAGTATCGCTTCTATCGCCAGTAAATCTGCCGGACCTGGCACGCGTGCCAATATCGATGAATTTACGGAAACCACTTCCCGAGCAAtt               <  1:866664/149‑1 (MQ=255)
                                                                                                                                       tgccgccaCCATTGGAATGGTGGCGGCAGTTTCACGCGTGGCGCGTGTTCATTACGCCGAAATTATCGCTTCTATCGCCAGTAAATCTGCCGGACCTGGCCCGCGGGCCAATATCGAGGAATTTACGGAAACCACTTCCCGAGCCATTg              >  1:814237/5‑149 (MQ=255)
                                                                                                                                       tgccgccaCCATTGGAATGGTGGCGGCAGTTTCACGCGTGGCGCGTGTTCATTACGCCGAAATTATCGCGTCTATCGCCAGTACATCTGCCGGACCTGGCACGCGTGACAATATCGAAGAATTTACGGAACCCACTTCCGGAGCCATTg              >  2:672604/5‑149 (MQ=255)
                                                                                                                                         aggcgaCCATTCCAATGGTGGCGGCAGTTTCACGGGTGGCGCGTGTTCATTACGCCGAAATTATCGCTTCTATCGCCAGTAAATCTGCCGGACCTGGCACGCGTGCCAATATCGATGAATTTACGGAAACCACTTCCCGAGCCATTGaa            <  1:223356/149‑1 (MQ=255)
                                                                                                                                         aggccaCCATTCCAATGGTGGCGGCAGTTTCACGCGTGGCGCGTGTTCATTACGCCGTAATTATCTCTTCTATCGCCAGGAAATCTGCCGGACCTGGCACGCGTGCCATTATCTATGAATTTACGGAAACCACTTCCCGCGCCATTGaa            >  2:725299/1‑149 (MQ=255)
                                                                                                                                              accaTTCCAATGGTGGCGGCAGTTTCACGCGTGGCGCGTGTTCATTACGCCGAAATTATCGCTTCTATCGCCAGTAAATCTGCCGGACCTGGCACGCGTGCCAATATCGATGAATTTACGGAAACCACTTCCCGAGCCATTGAAgtggt       >  1:213513/1‑149 (MQ=255)
                                                                                                                                              accaTTCCAATGGTGGCGGCAGTTTCACGCGTGGCGCGTGTTCATTACGCCGAAATTATCGCTTCTATCGCCAGTAAATCTGCCGGACATGGCACGCGTGCCAATATCGATGAATTTAAGGAAACCACTTCCCGAGCCAGTGAAgtggt       >  1:884756/1‑149 (MQ=255)
                                                                                                                                                  ttCCAATCGTGGCGGCAGTTTCACGCGTGGCGCGTGTTCATTACGCCGAAATTATCGCTTCTATCGCCAGTAAATCTGCCGGACCTGGCACGCGTGCCAATATCGATGAATTTACGGAAACCACTACCCGAGCCATTGAACTGGTGggc   >  2:630676/1‑149 (MQ=255)
                                                                                                                                                   tCCAATGGTGGCGGCAGTTTCACGCGTGGCGCGAGTTCATTACGCCGAAATTATCGCTTCTATCGCCAGTAAATCTGCCTGACCTGGCAAGCGTGCCAATATCGAAGAATTTACGGAAACAACTTCCCGAGCCATTGAAGTGGTGggcg  >  2:1002499/1‑149 (MQ=255)
                                                                                                                                                   tCCAATGGGGGCGGCAGGTGCACGCGTGGCGCGTGTTCATTACGCCGAAATTCTCGCTTCTATCGCCAGTAAATCTGCCGGACCTGGCACGCGTGCCAATATCGAGGAATTTACGGAAACCACTTCCCGAGCCATTGACGTGGTGggcg  <  2:459754/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
CGCTTTACGCGAAGCGAAACCGGATATTCGCTTAATTGACCTGACGCCTGCTGCCATCGGCCCTTACTGCGTGCCGGTGGTTAACCTCGAGGCGAACGTCGATCAACTGAACGTCAACATGGTCACCTGCGGCGGCCAGGCCACCATTCCAATGGTGGCGGCAGTTTCACGCGTGGCGCGTGTTCATTACGCCGAAATTATCGCTTCTATCGCCAGTAAATCTGCCGGACCTGGCACGCGTGCCAATATCGATGAATTTACGGAAACCACTTCCCGAGCCATTGAAGTGGTGGGCG  >  NC_000913/373184‑373479

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: