Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,309,402 C→T 6.4% W23* (TGG→TAG)  alsC ← D‑allose ABC transporter permease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,309,4020CT 6.4% 197.8 / 2.2 76W23* (TGG→TAG) alsCD‑allose ABC transporter permease
Reads supporting (aligned to +/- strand):  ref base C (33/38);  new base T (2/3);  total (35/41)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GAAAAACTCGCCCATGCCGATCAATACCGTCACGGAGCTTTGAACAAAAATCTGGGTAATATTATTGGTGGTCAGAAAATATTCTGGTGACAGCGAACCAAAGATGGCGACGATGATCGCCAGGATAAAAAAGGTGCCGTATTTATCCCAGAACAGCGCAAAGTTGAACGGTTTCTTCTCGCTCGCTTCGCTTTTTACTCTTGTGGTAAAGCCCATGCCATAATCTCCTCTTCGCTCATGTCATCGCGATTCGTCAGGATTTGCGTCAGTCGTCCTTCGCAGAACACGGCGATG  >  NC_000913/4309254‑4309547
                                                                                                                                                    |                                                                                                                                                 
gAAAAACTCGCCCATGCCGATCAATACCGTCACGGAGCTTTGAACAAAAATCTGGGTAATATTATTGGTGGTCAGAAAATATTCTGGTGACAGCGAACCAAAGATGGCGACGATGATCGCCAGGATAAAAAAGGTGCCGTATTTATccc                                                                                                                                                   >  2:840713/1‑149 (MQ=255)
 aaaaaCTCGCCCATGCCGATCAATACCGTCACGGCGCTTTGAACAAAAATCTGGGTAATATTATTGGTGGTCAGAAAATATTCTGGTGACAGCGAACCAAAGATGGCGACGATGATCGCCAGGATAAAAAAGGTGCCGTATTTATCCCa                                                                                                                                                  >  1:198410/1‑149 (MQ=255)
 aaaaaCTCGCCCATGCCGATCAATACCGTCACGGAGCTTTGAACAAAAATCTGGGTAATATTATTGGTGGTCAGAAAATATTCTGGTGACAGCGAACCAAAGATGGCGACGATGATCGCCAGGATAAAAAAGGTGCCGTATTTATCCCa                                                                                                                                                  >  2:431688/1‑149 (MQ=255)
  aaaaCTCGCCCATGCCGATCTATACCGTCACGGAGCTTTGAACAAAAATCTGGGTAATATTATTGGTGGTCAGAAAATATTCTGGTGACAGCGAACCAAAGATGGCGACGATGATCGCCAGGATAAAAAAGGTGCCGTATTTATCCCAg                                                                                                                                                 <  2:513763/149‑1 (MQ=255)
      cTCGCCCATGCCGATCAATACCGTCACGGAGCTTTGAACAAAAATCTGGGTAATATTATTGGTGGTCAGAAAATATTCTGGTGACAGCGAACCAAAGATGGCGACTATGATCGCCAGGATAAAAAAGGTGCCGTATTTATCCCAGAACa                                                                                                                                             >  1:728203/1‑149 (MQ=255)
         gCCCATTCCGATCAATAACGTCACGGAGCTTTGAACAAAAATCTGGGTAATATTATTGTTGGTCAGATAATATTCTTCTGACAGCGAACCAAAGATGGCGACGATGATCGACAGGATAAAAAAGGTGCCGTATTTATCACAGAACAgcc                                                                                                                                          >  2:925580/1‑148 (MQ=255)
             atcccGATCATTACCGTCACGGAGCTTTGAACAATAATCTGCGTAATTTTATTGGTGGTCAGAAAATATTCTGGTGACAGCGAACCAAAGATGGCGACGATGATCTCCAGGAAAAAAAAGGTGCCGTATTTATCCCAGAACAGCGCaaa                                                                                                                                      <  2:702241/146‑1 (MQ=255)
               gCCGATCAATACCGTCACGGAGCTTTGAACAAAAATCTGGGTAATATTATTGGTGGTCAGAAAATATTCTGGTGACAGCGAACCAAAGATGGCGACGATGATCGCCAGGATAAAAAAGGTGCCGTATTTATCCCAGAACAGCGCAAAGt                                                                                                                                    >  1:407599/1‑149 (MQ=255)
                ccGATCAATACCGTCACGGAGCTTTGAACAAAAATCTGGGTAATATTATTGGTGGTCAGAAAATATTCTGGTGACAGCGAACCAAAGATGGCGACGATGATCGCCAGGATAAAAAAGGTGCCGTATTTATCCCAGAACAGCGCAAAGtt                                                                                                                                   <  1:170711/149‑1 (MQ=255)
                  gATCAATACCGTCACGGAGCTTTGAACAAAAATCTGGGTAATATTATTGGTGGTCAGAAAATATTCTGGTGACAGCGAACCAAAGATGGCGACGATGATCGCCAGGATAAAAAAGGTGCCGTATTTATCCTAGAACAGCGCAAAGTTGa                                                                                                                                 >  1:339602/1‑149 (MQ=255)
                  gATCAATACCGTCACGGAGCTTTGAACAAAAATCTGGGTAATATTATTGGTGGTCAGAAAATATTCTGGTGACAGCGAACCAAAGATGGCGACGATGATCGCCAGGATAAAAAAGGTGCCGTATTTATCCTAGAACAGCGCAAAGTTGa                                                                                                                                 >  1:748627/1‑149 (MQ=255)
                   cacAATACCGTCACGGAGCTTTGAACAAAAATCTGGGCATTATTATTGGTGGTCAGAAAATATTCTGGTGACAGCGAACCAAAGATGGCGACGATGATCGCCAGGATAAAAAAGGTGCCGTATATATCCCAGAACAGCGCAAAGTTGaa                                                                                                                                <  2:187464/147‑1 (MQ=255)
                    tCAATACCGTCACGGATCTTTGAACAAAAATCTGGGTAATATTATTGGTGGTCAGAAAATATTCTGGTTACAGCGAACCAAAGATGGCGACGATGATCGCCAGGATAAAAAAGGTGCCGTATTTATCCCAGAACAGCGCATAGTTGAAc                                                                                                                               <  1:233184/149‑1 (MQ=255)
                         aCCGTCACGGAGCTTTGAACAAAAATCTGGGTAATATTATTGGTGGTCAGAAAATATTCTGGTGACAGCGAACCAAAGATGGCGACGATGATCGCCAGGATAAAAAAGGTGCCGTATTTATCCCAGAACAGCGCAAAGTTGAACGGttt                                                                                                                          <  1:405624/149‑1 (MQ=255)
                          ccGTCACGGAGCTTTGAACAAAAATCTTGGTAATATTATTGGTGGTCATAAAATAATCTGGTTACAGCGAACCAAAGTTGTCTACGATGATCGCCATGATAAAAAAGGTGCCGTATTTATCCCAGAACAGCGCAAAGTTGAACGGTttc                                                                                                                         <  2:146912/149‑1 (MQ=255)
                              cACGGAGCTTTGAACAAAAATCTGGGTAATATTATTGGTTGGCAGAAAATATTCTGGTGACAGCGAACCAAAGAGGGCGACGATGATCGCCAGGATAAAAAAGGTGCCGTATTTATCCCAGAACAGCGCAAAGTTGAACGGTTtcttct                                                                                                                     <  1:369492/149‑1 (MQ=255)
                                 ggAGCTTTCAACAAAAATCTGGGTAATATTATTGGTGGTCAGAAAATATTCTGGTGACAGCGAACCAAAGATGGCGACGATGATCGCCAGGATAAAAAAGGTGCCGTATTTATCCCAGAACAGCGCAAAGTTGTACGGTTTCTTctcga                                                                                                                  >  2:819887/1‑148 (MQ=255)
                                      tttGAACAAAAATCTGGGTAATATTATTGGTGGTCAGAAAATATTCTGGTGCCAGCGAACCAAAGATGGCGACGATGATCGCCAGGATAAAAAAGGTGCCGTATTTATCCCAGCACAGCGCAAAGTTGAACGGTTTCTTCTcgctcgct                                                                                                             >  2:139705/1‑149 (MQ=255)
                                       ttGAACAAAAATCTGGGTAATATTATTGGTGGTCAGAAAATATTCTGGTGACAGCGAACCAAAGATGGCGACGATGATCGCCAGGATAAAAAAGGTGCCGTATTTATCCCAGAACAGCGCAAAGTTGAACGGTTTCTTCTCGCtcgctt                                                                                                            >  1:47940/1‑149 (MQ=255)
                                          aaCAAAAATCTGGGTAATATTATTGGTGGTCAGAAAATATTCTGGTGACAGCGAACCAAAGATGGCGACGATGATCGCCAGGATAAAAAAGGTGCCGTATTTATCCCAGAACAGCGCAAAGTTGAACGGTTTCTTCTCGCtcgcttcgc                                                                                                         >  1:715859/1‑149 (MQ=255)
                                          aaCAAAAATCTGGGTAATATTATTGGTGGTCAGAAAATATTCTGGTGACAGCGAACCAAAGATGGCGACGATGAGCGCCAGGATAAAAAAGGTGCCGTATTTATCCCAGAACAGCGCAAAGTTGAACGGTTTCTTCTCGCtcgcttcgc                                                                                                         <  2:551606/149‑1 (MQ=255)
                                             aaaaaTCTGTGTAATAATATTGGTGGTCAGAAAATATTCTGGTGACAGCGAACCAAAGATGGCGACGATGATCGCCAGGATAAAAAAGGTGCCGTATTTATCCCAGAACCGCGCAAAGTTGAACGGTTTCTTCTCGCTCGCTTCGCttt                                                                                                      <  1:253627/149‑1 (MQ=255)
                                             aaaaaTCTGGGTAATATTATTGGTGGTCAGAAAATATTCTGGTGACAGCGAACCAAAGATGGCGACGATGATCGCCAGGATAAAAAAGGTGCCGTATTTATCCCAGAACAGCGCAAAGTTGAACGGTTTCTTCTCGCTCGCTTCTCttt                                                                                                      <  2:320370/149‑1 (MQ=255)
                                             aaaaaTCTGGGTAATAATATTGGTGGTAATAAAATATTCTGGTGACAGCGAACCAAAGATGGCGACGATGATCGCCAGGATAAAAAAGGTGCCGTATTTATCCCAGAACAGCGCAAAGTTGATCGGTTTCTTCTCGCGcgcttcgctta                                                                                                      <  2:812893/149‑2 (MQ=255)
                                               aaaTCTGGGTAATATTATTGGTGGTCAGAAAATATTCTGGTGACAGCGAACCAAAGATGGCGACGATGATCGCCAGGATAAAAAAGGTGCCGTATTTTTCCCAGAACAGCGCAAAGTTGAACGGTTTCTTCTCGCTCGCTTCGCttttt                                                                                                    >  1:532028/1‑149 (MQ=255)
                                               aaaTCTGGGTAATAATATTGGTGGTCAGAAAATATTCTGGTGACAGCGAACCAAAGATGGCGACGATGATCGCCAGGATAAAAAAGGTGCCGTATTTATCCCAGAACAGCGCAAAGTTGAACGGTTTCTTCCCGCTCGCTTCGCttttt                                                                                                    <  1:540196/149‑1 (MQ=255)
                                                aaTCTGGGTAATATTATTGGTGGTCAGAAAATATTCTGGTGACAGCGAACCAAAGATGGGGACGATGATCGCCAGGATAAAAAAGGTGCCGTATTTATCCCAGAACAGCGCAAAGTTGAACGGTTTCTTCTCGCTCGCTTCGCTTTTTa                                                                                                   <  1:590322/149‑1 (MQ=255)
                                                   cTGGGTACTATTATTGGTGGTCCGAAAATATTCTGGAGACAGGGAACCATAGATGGCGACGATGATCGCCAGGATAAAAAAGGTGCCGTATTTATCCCAGAACAGCGCAAAGTTGAACGGTATCTTCTCGCGCGCTTCGCTTTTTCctc                                                                                                <  1:260372/149‑1 (MQ=255)
                                                     gggTAATATTATTGGTGGTCAGAAAATATTCTGGTGCCAGCGAACCAAAGATGGCGACGATGATCGCCAGGATAAAAAAGGTGCCGTATTTATCCCAGAACAGCGCAAAGTTGAACGGTTTCTTCTCGCTCGCTTCGCTTTTTACTCtt                                                                                              >  2:456122/1‑149 (MQ=255)
                                                     gggTAATATTATTGGTGGTCAGAAAATATTCTGGTGACAGCGAACCAAAGATGGCGACGATGATCGCCAGGATAAAAAAGGTGCCGTATTTATCCCAGAACAGCGCAAAGTTGAACGGTTTCTTCTCGCTCGCTTCGCTTTTTACTCtt                                                                                              <  2:586821/149‑1 (MQ=255)
                                                      ggTAATATTATTGGTGGTCAGAAAATATTCTGGTGACAGCGAACCAAAGATGGCGACGATGATCGCCAGGATAAAAAAGGTGCCGTATTTATCCCAGAACAGCGCAAAGTTGAACGGTTTCTTCTCGCTCGCTTCGCTTTTTACTCTtg                                                                                             >  1:391421/1‑149 (MQ=255)
                                                      ggTAATATTATTGGTGGTCAGAAAATATTCTGGTGACAGCGAACCAAAGATGGCGACGAGGATCGCCAGGATAAAAAAGGTGCCGTATTTATCCCAGACCAGCGCAAAGTTGAACGGTTTCTTCTCGCTCGCTTCGCTTTTTACTCTtg                                                                                             >  1:721095/1‑149 (MQ=255)
                                                       gTACTATTATTGGCGGGCAGAAAATATTCTGCGGACAGCGAACCAAAGATGGCGACGATGATCTCCAGGATAAAAAAGGTGCCGTATTTATCCCAGAACAGCGCAAAGTTGAACGGTGTCTTCTCGCTCGCTTCGCTTTTTACTCTtgt                                                                                            <  2:721095/149‑1 (MQ=255)
                                                       gTAATATTATTGGTGGTCAGAAAATATTCTGGTGACAGCGAACCAAAGATGGCGACGATGATCGCCAGGATAAAAAAGGTGCCGTATTTATCCCAGAACAGCGCAAAGTTGAACGGTTTCTTCTCGCTCGCTTCGCTTTTTACTCTtgt                                                                                            <  2:391421/149‑1 (MQ=255)
                                                        tAATATTATTGGTGGTCAGAAAATATTCTGGTGACAGCGAACCAAAGATGGCGACGATGATCGCCAGGATAAAAAAGGTGCCGTATTTATCCTAGAACAGCGCAAAGTTGAACGGTTTCTTCTCGCTCGCTTCGCTTTTTACTCTtgtg                                                                                           <  1:213867/149‑1 (MQ=255)
                                                        tAATATTATTGGTGGTCAGAAAATATTCTGGTGACAGCGAACCAAAGATGGCGACGATGATCGCCAGGATAAAAAAGGTGCCGTATTTATCCCAGAACAGCGCAAAGTTGAACGGTTTCTTCTCGCTCGCTTCGCTTTTTACTCTtgtg                                                                                           >  1:276641/1‑149 (MQ=255)
                                                        tAAAATTATAGGTGGTCAGAAAATATTCTGGTGACACCGAACCAAAGATGGCGACGATGATCGCCAGGATAAAAAAGGTGCCGTATTTATCCCAGAACAGCTCAAAGTTGAACGGTTTCTTCTCGCTCGCTTCGCTTTTTACTCTtgtg                                                                                           <  2:358243/149‑1 (MQ=255)
                                                                   gtggtCAGAAAATATTCTGGTGACAGCGAACCAAAGATGGCGACGATGATCGCCAGGATAAAAAAGGTGCCGTATTTATCCCAGAACAGCACAAAGTTGAACGGTTTCTTCTCGCTCGCTTCGCTTTTTACTCTTGTGGTAAAGCCCAt                                                                                >  1:216159/1‑149 (MQ=255)
                                                                    tggtCAGAAAATATTCTGGTGACAGCGAACCAAAGATGGCGACGATGATCGCCAGGATAAAAAAGGTGCCGTATTTATCCCAGAACAGCGCAAAGTTGAACGGTTTCTTCTCGCTCGCTTCGCTTTTTACTCTTGTGGTAAAGCCCATg                                                                               >  2:596252/1‑149 (MQ=255)
                                                                       tCAGAAAATATTCTGGTGACAGCGAACCAAAGATGGCGACGATGATCGCCAGGATAAAAAAGGTGCCGTATTTATCCCAGAACAGCGCAAAGTTGAACGGTTTCTTCTCGCTCGCTTCGCTTTTTACTCTTGTGGTAAAGCCCATGCCa                                                                            >  1:732546/1‑149 (MQ=255)
                                                                         aGAAAATATTCTGGTGACAGCGAACCAAAGATGGCGACGATGATCGCCAGGATAAAAAAGGTGCCGTATTTATCCCAGAACAGCGCAAAGTTGAACGGTTTCTTCTCGCTCGCTTCGCTTTTTACTCTTGTGGTAAAGCCCATGCCATa                                                                          >  1:771841/1‑149 (MQ=255)
                                                                         aGAAAATATTCTGGTGACAGCGAACCAAAGATGGCGACGATGATCGCCAGGATAAAAAAGGTGCCGTATTTATCCCAGAACAGCGCAAAGTTGAACGGTTTCTTCTCGCTCGCTTCGCTTTTTACTCTTGTGGTAAAGCCCATGCCATa                                                                          >  1:653414/1‑149 (MQ=255)
                                                                         aGAAAATATTCTGGTGACAGCGAACCAAAGATGGCGACGATGATCGCCAGGATAAAAAAGGTGCCGTATTTATCCCAGAACAGCGCAAAGTTGAACGGTTTCTTCTCGCTCGCTTCGCTTTTTACTCTTGTGGTAAAGCCCATGCCATa                                                                          >  2:484502/1‑149 (MQ=255)
                                                                          gAAAATATTCTGGTGACAGCGAACCAAAGATGGCGACGAGGATCGCCAGGATAAAAAAGGTGCCGTATTTATCCCAGAACAGCGCAAAGTTGAACGGTTGCTTCTCGCTCGCGTCGCTGTTTACTCTTGTGGTAAAGCCCATGCCATaa                                                                         >  1:984271/1‑149 (MQ=255)
                                                                            aaaTATTCTGGTGACAGCGAACCAAAGATGGCGACGATGATCGCCAGGATAAAAAAGGTGCCGTATTTATCCCAGAACAGCGCAAAGTTGAACGGTTTCTTCTCGCTCGCTTCGCTTTTTACTCTTGTGGTAAAGCCCATGCCATAAtc                                                                       <  2:47940/149‑1 (MQ=255)
                                                                               tatTCTGGTGACAGCGAACCAAAGATGGCGACGATGATCGCCAGGATAAAAAAGGTGCCGTATTTATCCCAGAACAGCGCAAAGTTGAACGGTTTCTTCTCGCTCGCTTCGCTTTTTACTCTTGTGGTAAAGCCCATGCCATAATctcc                                                                    >  2:491348/1‑149 (MQ=255)
                                                                                atTCTGGTGACAGCGAACCAAAGATGGCGACGATGATCGCCAGGATAAAAAAGGTGCCGTATTTATCCCAGAACAGCGCAAAGTTGAACGGTTTCTTCTCGCTCGCTTCGCTTTTTACTCTTGTGGTAAAGCCCATGCCATAATctcct                                                                   <  2:734898/149‑1 (MQ=255)
                                                                                atTCTGGTGACAGCGAACCAAAGATGGCGACGATGATCGCCAGGATAAAAAAGGTGCCGTATTTATCCCAGAACAGCGCAAAGTTGAACGGTTTCTTCTCGCTCGCTTCGCTTTTTACTCTTGTGGTAAAGCCCATGCCATAATctcct                                                                   >  2:397710/1‑149 (MQ=255)
                                                                                 ttCTGGTGACAGCGAACCAAAGATGGCGACGATGATCGCCAGGATAAAAAAGGTGCCGTATTTATCCCAGAACAGCGCAAAGTTGAACGGTTTCTTCTCGCTCGCTTCGCTTTTTACTCTTGTGGTAAAGCCCATGCCATAATctcctc                                                                  >  1:861498/1‑149 (MQ=255)
                                                                                    tGGTGACAGCGAACCAAAGATGGCGACGATGATCGCCAGGATAAAAAAGGTGCCGTATTTATCCCAGAACAGCGCAAAGTTGAACGGTTTCTTCTCGCTCGCTTCGCTTTTTACTCTTGTGGTAAAGCCCATGCCATAATCTCCTCTTc                                                               <  2:407599/149‑1 (MQ=255)
                                                                                              gAACCAAAGATGGCGACGATGATCGCCAGGATAAAAAAGGGGCCGTATTTATCCCAGAACAGCGCAAAGTTGAACGGTTTCTTCTCGCTCGCTTCGCTTTTTACTCTTGTGGTAAAGCCCATGCCATAATCTCCTCTTCGCTCATGTCa                                                     <  2:3425/149‑1 (MQ=255)
                                                                                                  cAAAGATGGCGACGATGATCGCCAGGATAAAAAAGGTGCCGTATTTATCCCAGAACAGCGCAAAGTTGAACGGTTTCTTCTCGCTCGCTTCGCTTTTTACTCTTGTGGTAAAGCCCATGCCATAATCTCCTCTTCGCTCATGTCATcgc                                                 <  2:715859/149‑1 (MQ=255)
                                                                                                  cAAAGATGGCGACGATGATCGCCAGGATAAAAAAGGTGCCGAATTTATCCCAGAACAGCGCAAAGTTGAACGGTTTCTTCTCGCTCGCTTCGCTTTTTACTCTTGTGGTAAAGCCCATGCAATAATCTCCTCTTCGCTCATGTCATcgc                                                 >  2:265272/1‑149 (MQ=255)
                                                                                                    aaGATGGCGACGATGATCGCCAGGATAAAAAAGGTGCCGTATTTATCCCAGAACAGCGCAAAGTTGAACGGTTTCTTCTCGCTCGCTTCGCTTTTTACTCTTGTGGTAAAGCCCATGCCATAATCTCCTCTTCGCTCATGTCATCGCGa                                               <  1:170676/149‑1 (MQ=255)
                                                                                                       aTGGCGACGATGATCGCCAGGATAAAAAAGGTGCCGTATTTATCCTAGAACAGCGCAAAGTTGAACGGTTTCTTCTCGCTCGCTTCGCTTTTTACTCTTGTGGTAAAGCCCATGCCATAATCTCCTCTTCGCTCATGTCATCGCGATTc                                            <  2:339602/149‑1 (MQ=255)
                                                                                                       aTGGCGACGATGATCGCCAGGATAAAAAAGGTGCCGTATTTATCCTAGAACAGCGCAAAGTTGAACGGTTTCTTCTCGCTCGCTTCGCTTTTTACTCTTGTGGTAAAGCCCATGCCATAATCTCCTCTTCGCTCATGTCATCGCGATTc                                            <  2:748627/149‑1 (MQ=255)
                                                                                                         ggCGACGATGATCGCCAGGATAAAAAAGGTGCCGTATTTATCCCAGAACAGCGCAAAGTTGAACGCTTTCTTCTCGCTCGCTTCGCTTTTTACTCTTGTGGTAAAGCCCATGCCATAATCTCCTCTTCGCTCATTTCATCGCGATTCGt                                          >  1:947327/1‑149 (MQ=255)
                                                                                                           cgacgaTGATCGCCAGGATAAAAAAGGTGCCGTATTTATCCCAGAACAGCGCAAAGTTGAACGGTTTCTTCTCGCTCGCTTCGCTTTTTACTCTTGTGGTAAAGCCCATGCCATAATCTCCTCTTCGCTCATGTCATCGCGATTCGTCa                                        >  2:862863/1‑149 (MQ=255)
                                                                                                           cgacgaTGATCGCCAGGATAAAAAAGGTGCCGTATTTATCCCAGAACAGCGCAAAGTTGAACGGTTTCTTCTCGCTCGCTTCGCTTTTTACTCTTGTGGTAAAGCCCATGCCATAATCTCCTCTTCGCTCATGTCATCGCGATTCGTCa                                        <  1:95015/149‑1 (MQ=255)
                                                                                                           cgacgaTGATCGCCAGGATAAAAAAGGTGCCGTATTTATCCCAGAACAGCACAAAGTTGAACGGTTTCTTCTCGCTCGCTTCGCTTTTTACTCTTGTGGTAAAGCCCATGCCATAATCTCCTCTTCGCTCATGTCATCGCGATTCGTCa                                        <  2:216159/149‑1 (MQ=255)
                                                                                                                  gatCGCCAGGATAAAAAAGGTGCCGTATTTATCCCAGAACAGCGCAAAGTTGAACGGTTTCTTCTCGCTCGCTTCGCTTTTTACTCTTGTGGTAAAGCCCATGCCATAATCTCCTCTTCGCTCATGTCATCGCGATTCGTCAGGATTTg                                 <  1:941784/149‑1 (MQ=255)
                                                                                                                  gatCGCCAGGATAAAAAAGGTGCCGTATTTATCCCAGAACAGCGCAAAGTTGAACGGTTTCTTCTCGCTCGCTTCGCTTTTTACTCTTGTGGTAAAGCACATGCCATAATCTCCTCTTCGCTCATGTCATCGCGATTCGTCAGGAtttt                                 >  1:228122/1‑148 (MQ=255)
                                                                                                                   atCGCCAGGATAAAAAAGGTGCCGTATTTATCCCAGAACAGCGCAAAGTTGAACGGTTTCTTCTCGCTCGCTTCGCTTTTTACTCTTGTGGTAAAGCCCATGCCATAATCTCCTCTTCGCTCATGTCATCGCGATTCGTCAGGATTTGc                                <  2:543143/149‑1 (MQ=255)
                                                                                                                   atCGCCAGGATAAAAAAGGTGCCGTATTTATCCCAGAACAGCGCAAAGTTGAACGGTTTCTTCTCGCTCGCTTCGCTTTTTACTCTTGTGGTAAAGCCCATGCCATAATCTCCTCTTCGCTCATGTCATCGCGATTCGTCAGGATTTGc                                <  1:397710/149‑1 (MQ=255)
                                                                                                                   atCGCCAGGATAAAAAAGGTGCCGTATTTATCCCAGAACAGCGCAAAGTTGAACGGTTTCTTCTCGCTCGCTTCGCTTTTTACTCTTGTGGTAAAGCCCATGCCATAAACTCCTCTTCGCTCATGTCATCGCGATTCGTCAGGATTTGc                                >  2:236752/1‑149 (MQ=255)
                                                                                                                   atCGCCAGGATAAAAAAGGTGCCGTATATATCCCAGAACAGCGCAAAGATGAACGGTTTCTTCTCGCTCGCTTCGCTTTTTACTCTAGTGGTAAAGCCCATGCCATAATCTCCTCTTCGCTCATGTCATCGCGATTCGTCAGGATTTGc                                <  1:825969/149‑1 (MQ=255)
                                                                                                                      gCCAGGATAAAAAAGGTGCCGTATTTATCCCAGAACAGCGCAAAGTTGAACGGTTTCTTCTCGCTCGCTTCGCTTTTTACTCTTGTGGTAAAGCCCATGCCATAATCTCCTCTTCGCTCATGTCATCGCGATTCGTCAGGATTTGCGTc                             <  2:276641/149‑1 (MQ=255)
                                                                                                                          ggATAAAAAAGGTGCCGTATTTATCCCAGAACAGCGCAAAGTTGAACGGTTTCTTCTCGCTCGCTTCGCTTTTTACTCTTGTGGTAAAGCCCATGCCATAATCTCCTCTACGCTCATGTCATCGCGATTCGTCAGGATTTGCGTCAgtc                         >  1:521607/1‑149 (MQ=255)
                                                                                                                               aaaaaGGTGCCGTATTTATCCCAGAACAGCGCAAAGTTGAACGGTTTCTTCTCGCTCGCTTCGCTTTTTACTCTTGTGGTAAAGCCCATGCCATAATCTCCTCTTCGCTCATGTCATCGCGATTCGTCAGGATTTGCGTCAGTCGTCCt                    <  1:840713/149‑1 (MQ=255)
                                                                                                                                 aaaGGTGCCGTATTTATCCCAGAACAGCGCAAAGTTGAACGGTTTCTTCTCGCTCGCTTCGCTTTTTACTCTTGTGGTAAAGCCCATGCCATAATCTCCTCTTCGCTCATGTCATCGCGATTCGTCAGGATTTGCGTCAGTCGTCCTTc                  <  1:444172/149‑1 (MQ=255)
                                                                                                                                    ggTGCCGTATTTATCCCAGAACAGCGCAAAGTTGAACGGTTTCTTCTCGCTCGCTTCGCTTTTTACTCTTGTGGTAAAGCCCATGCCATAATCTCCTCTTCGCTCATGTCATCGCGATTCGTCAGGATTTGCGTCAGTCGTCCTTCGCa               <  2:198410/149‑1 (MQ=255)
                                                                                                                                    ggTGCCGTATTTATCCCAGAACAGCGCAAAGTTGAACGGTTTCTTCTCGCTCGCTTCGCTTTTTACTCTTGTGGTAAAGCCCATGCCATAATCTCCTCTTCGCTCATGTCATCGCGATTCGTCAGGATTTGCGTCAGTCGTCCTTCGCa               <  1:484502/149‑1 (MQ=255)
                                                                                                                                       gCCGTATTTATCCCAGAACAGCGCAAAGTTGAACGGTTTCTTCTCGCTCGCTTCGCTTTTTACTCTTGTGGTAAAGCCCATGCCATAATCTCCTCTTCGCTCATGTCATCGCGATTCGTCAGGATTTGCGTCAGTCGTCCTTCGCAGaa            <  1:377485/149‑1 (MQ=255)
                                                                                                                                           tATTTATCCCAGAACAGCGCAAAGTTGAACGGTTTCTTCTCGCTCGCTTCGCTTTTTACTCTTGTGGTAAAGCCCATGCCATAATATCCTCTTCGCTCATGTCATCGCGATTCGTCAGGATTTTCGTCAGTCGTCATTCGCAGAAAACg        >  1:899430/1‑149 (MQ=255)
                                                                                                                                            aTTTATCCCAGAACAGCGCAAAGTTGAACGGTTTCTTCTCGCTCGCTTCGCTTTTTACTCTTGTGGTAAAGACCATGCCATAATCTCCTCTTCGCTCATGTCATCGCGATTCGTCAGGATTTGCGTCAGTCGTCCTTCGAAGAACACgg       >  1:526142/1‑149 (MQ=255)
                                                                                                                                                aTCCCAGAACAGCGCAAAGTTGAACGGTTTCTTCTCGCTCGCTTCGCTTTTTACTCTTGTGGTAAAGCCCATGCCATAATCTCCTCTTCGCTCATGTCATCGCGATTCGTCAGGATTTGCGTCAGTCGTCCTTCGCAGAACACGGCGAt   <  2:302233/149‑1 (MQ=255)
                                                                                                                                                 tCCCAGAACAGCGCAAAGTTGAACGGTTTCTTCTCGCTCGCTTCGCTTTTTACTCTTGTGGTAAAGCCCATGCCATAATCTCCTCTTCGCTCATGTCATCGCGATTCGTCAGGATTTGCGTCAGTCGTCCTTCGCAGAACACGGCGATg  >  2:452739/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                 
GAAAAACTCGCCCATGCCGATCAATACCGTCACGGAGCTTTGAACAAAAATCTGGGTAATATTATTGGTGGTCAGAAAATATTCTGGTGACAGCGAACCAAAGATGGCGACGATGATCGCCAGGATAAAAAAGGTGCCGTATTTATCCCAGAACAGCGCAAAGTTGAACGGTTTCTTCTCGCTCGCTTCGCTTTTTACTCTTGTGGTAAAGCCCATGCCATAATCTCCTCTTCGCTCATGTCATCGCGATTCGTCAGGATTTGCGTCAGTCGTCCTTCGCAGAACACGGCGATG  >  NC_000913/4309254‑4309547

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: