Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,406,129 T→C 100% intergenic (+144/‑61) purA → / → nsrR adenylosuccinate synthetase/nitric oxide‑sensitive repressor for NO regulon

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,406,1290TC100.0% 292.2 / ‑6.7 109intergenic (+144/‑61)purA/nsrRadenylosuccinate synthetase/nitric oxide‑sensitive repressor for NO regulon
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base C (49/59);  minor base A (1/0);  total (50/59)
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 109 total aligned reads displayed.

TAATTCTGGTACGCCTGGCAGATATTTTGCCTGCCGGGCGAACAGTGTGATACATTGCTGTGTCGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCTTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGAGTTTCACTGATTACGGATTACGTGCGCTGATCTACATGGCGTCATTGCCAGAAGGGCGGATGACCAGTA  >  NC_000913/4405983‑4406271
                                                                                                                                                  |                                                                                                                                              
tAATTCTGGTACGCCTGGCAGATATTTTGCATGCCGGGCGAACAGTGTGATACATTGCTGTGTCGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCCtg                                                                                                                                              >  1:681781/1‑149 (MQ=255)
 aaTTCTGGTACGCCTGGCAGATATTTTGCCTGCCGGGCGAACAGTGTGATACATTGCTGTGTCGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCCtgt                                                                                                                                             >  1:301093/1‑149 (MQ=255)
 aaTTCTGGTACGCCTGGCAGATATTTAGACTGCCGGGCGAACAGTGTGATACATTGCTGTGTCGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTCAAATAAATTAGCGACACAGCCtgt                                                                                                                                             <  2:88382/149‑1 (MQ=255)
    tCTGGTACGCCTGGCAGATATTTTGCCTGCCGGGCGATCAGTGTGATACATTGCTGTGTCGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTGTAATAAATTAGCGACACAGCCTGTGGc                                                                                                                                          <  2:403215/149‑1 (MQ=255)
      tGGTACGCCTGGCAGATATTTTGCCTGCCGGGCGAACAGTGTGATACATTGCTGTGTCGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTg                                                                                                                                        <  2:1000917/149‑1 (MQ=255)
       ggTACGCCTGGCAGATATTTTGCCTGCCGGGCGAACAGTGTGATACATTGCTGTGTCGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTgg                                                                                                                                       <  2:384426/149‑1 (MQ=255)
         tACGCCTGGCAGATATTTTGCCTGCCGGGCGAACAGTGTGATACATTGCTGTGTCGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTGAGATAAATTAGCGACACAGCCTGTGGCTGGtt                                                                                                                                     <  2:408791/149‑1 (MQ=255)
         tACGCCTGGCAGATATTTTGCCTGCCGGGCGAACAGTGTGATACATTGCTGTGTCGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGtt                                                                                                                                     >  1:442971/1‑149 (MQ=255)
           cGCCTGGCAGATATTTTGCCTGCCGGGCGAACAGTGTGATACATTGCTGTGTCGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTa                                                                                                                                   >  1:269808/1‑149 (MQ=255)
            gCCTGGCAGATATTTTGCCTGCCGGGCGAACAGTGTGATACATTGCTGTGTCGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTat                                                                                                                                  >  2:508766/1‑149 (MQ=255)
            gCCTGGCAGATATTTTGCCTGCCGGGCGAACAGTGTGATACATTGCTGTGTCGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTat                                                                                                                                  <  1:855057/149‑1 (MQ=255)
             ccTGGCATATATTTTGCCTGCCGGGCGAACAGTGTGATACATTGCTGTGTCGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTTAAATAAATTAGCGACACAGCCTGTGGCTGGTTTatc                                                                                                                                 <  1:365072/149‑1 (MQ=255)
             ccTGGCAGATATTTTGCCTGCCGGGCGAACAGTGTGATACATTGCTGTGTCGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTatc                                                                                                                                 <  2:711850/149‑1 (MQ=255)
               tGGCAGATATTTTGCCTGCCGGGCGAACAGTGTGATACATTGCTGTGTCGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTatcat                                                                                                                               >  1:486169/1‑149 (MQ=255)
                   aGATATTTTGCCTGCCGGGCGAACAGTGTGATACATTGCTGTGTCGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAat                                                                                                                           <  1:552583/149‑1 (MQ=255)
                     atatTTTGCCTGCCGGGCGAACAGTGTGATACATTGCTGTGTCGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGTCACAGCCTGTGGCTGGTTTATCATCAatat                                                                                                                         <  2:972242/149‑1 (MQ=255)
                       atTTTGCCTGCCGGGCGAACAGTGTGATACATTGCTGTGTCGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATaa                                                                                                                       <  1:1007599/149‑1 (MQ=255)
                        ttttGCCTGCCGGGCGAACAGTGTGATACATTGCTGTGTCGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATaaa                                                                                                                      <  2:486169/149‑1 (MQ=255)
                        ttttGCCTGCCGGGCGAACAGTGTGATACATTGCTGTGTCGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATaaa                                                                                                                      >  2:726068/1‑149 (MQ=255)
                        ttttGCCTGCCGGGCGAACAGTGTGATACATTGCTGTGTCGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCATCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATaaa                                                                                                                      <  1:506884/149‑1 (MQ=255)
                         tttGCCTGCCGGGCGAACAGTGTGATACATTGCTGTGTCGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAAt                                                                                                                     <  1:508766/149‑1 (MQ=255)
                                  ctggCGAACAGTGTGATACATTGCTGTGTCGGGTAAGCCATTACGCTATCCGCAACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTAtttttt                                                                                                            <  1:1010623/147‑1 (MQ=255)
                                  cGGGCGAACAGTGTGATACATTGCTGTGTCGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTAtttttt                                                                                                            >  1:678482/1‑149 (MQ=255)
                                   gggCGAACAGTGTGATACATTGCTGTGTCGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTc                                                                                                           <  1:540784/149‑1 (MQ=255)
                                    ggCGTACAGTGTGATACATTGCTGTGGCGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTGGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTcc                                                                                                          <  1:814534/149‑1 (MQ=255)
                                    ggCGAACAGTGTGATACATTGCTGTGTCGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTcc                                                                                                          <  2:874629/149‑1 (MQ=255)
                                        aaCAGTGTGATACATTGCTGTGTCGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATTTAATTTTTCACGAt                                                                                                      >  1:427751/1‑149 (MQ=255)
                                             tgtgATACATTGCTGTGTCGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTccc                                                                                                 <  2:305588/149‑1 (MQ=255)
                                                   aCATTGCTGTGTCGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGa                                                                                           >  1:837204/1‑149 (MQ=255)
                                                   aCATTGCTGTGTCGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGa                                                                                           >  1:458902/1‑149 (MQ=255)
                                                    cATTGCTGAGGCGTGTAAGCCATTACGCTATCCGACACAGTGTTAACTCCTCGCTTCTTTCCTTCCCCGAACTGAAATAAATTAGCGCCACAGCCTGTGGCTGGTTTATCCTCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAg                                                                                          <  2:574185/149‑1 (MQ=255)
                                                     aaagCTGTGTCGGCTAACCCATTCCTCTAACCTATACATAGTTAACACCTCTCTTTTATCATACCCCGAACTCTAATAAAATAGCCACACAGCCTGTGGCTGGTGTATCCTCAATATAAATGTATTTTTTCCCTATTTCCCTTTTGatg                                                                                         <  2:662987/146‑3 (MQ=255)
                                                         cTGTGTCGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGa                                                                                     >  2:239547/1‑149 (MQ=255)
                                                            tgtCGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGAtgt                                                                                  <  1:982196/149‑1 (MQ=255)
                                                            tgtCGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTGAAAAAAATTAGCGACACAGCCTGAGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGAtgt                                                                                  >  2:843742/1‑149 (MQ=255)
                                                             gtCGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGAtgtg                                                                                 <  2:681781/149‑1 (MQ=255)
                                                              tCGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGc                                                                                >  1:510288/1‑149 (MQ=255)
                                                               cGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCa                                                                               <  1:354426/149‑1 (MQ=255)
                                                                  gTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGtt                                                                            <  2:726160/149‑1 (MQ=255)
                                                                   tAAGCCATTACGCTATGCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTGATATAAATTAGCGACACAGGCTGTGGCGGGTTTATCTTCAGTATAATTGTATTTTTTCCCGATTTCCCTGTTGAGGTTGATGTGCAggtg                                                                           >  2:483944/1‑146 (MQ=255)
                                                                   tAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGATGTTCAGTTa                                                                           >  2:782388/1‑149 (MQ=255)
                                                                     aGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAAc                                                                         <  1:1029594/149‑1 (MQ=255)
                                                                     aGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAAc                                                                         <  1:482959/149‑1 (MQ=255)
                                                                        cATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGAg                                                                      >  1:1005945/1‑149 (MQ=255)
                                                                        cATTACGCTATCCGACACAGTGTGAACTCCTCGCTTTTTTCCGTCCCCGAACTGAAATAACTTAGCGACACAGCCTGTGGCTGGTTTATCATCAAGATAAATGTATTTTTTACCGATTTCCCTGTTGAGGTTGATGGGCAGTGAACGAg                                                                      >  2:797679/1‑149 (MQ=255)
                                                                         aTTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGAGt                                                                     <  1:910239/149‑1 (MQ=255)
                                                                           tACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTATTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGAGttt                                                                   <  2:492896/149‑1 (MQ=255)
                                                                             cGCTATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGtt                                                                                       >  2:5002/1‑127 (MQ=255)
                                                                               cTATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGGTCATGTGCAGTTAACGAGTTTCACt                                                               <  1:900051/149‑1 (MQ=255)
                                                                                  tcagACACAGTGTTAAATCCTCGATTTTTTCCTTCCCAGAACTGAAATAAATTAGCGACACAGCCTGAGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTTAGGTTGATGTGCAGTTAACGAGTTTCACTgat                                                            <  1:904977/146‑1 (MQ=255)
                                                                                    cGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGAGTTTCACTgatta                                                          <  1:678289/149‑1 (MQ=255)
                                                                                     gACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCCGGTGGCTGGTTTATCATCAATATAACTGTATTTTTTCCCTCTTTCCCTTTTGAGGGTGATGTGCAGTTAACGAGTTTCACTcaatac                                                         >  2:181865/1‑149 (MQ=255)
                                                                                      acacAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGAGTTTCACTgattacg                                                        <  2:347155/149‑1 (MQ=255)
                                                                                          aGTGTTAACTCCTCGCTTTTTTCCTTCCCCTAACTGAAATAAATTAGCGACACCGCCTGTGGCTGGTTTATCATCAATATACATGTATTTTTTCCCGATTTCCCTTTTGAGTTTGATGTGCAGTTAACGAGTTTCACTgattacggatt                                                    <  2:319635/149‑1 (MQ=255)
                                                                                          aGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCATTTTGAGGTTGATGTGCAGTTAACGAGTTTAACTgattacggatt                                                    >  1:562835/1‑149 (MQ=255)
                                                                                          aGTGTGAAATCCTCGCTGTTTTCCTACCCCGAACTGAAATAAAATATCGACACAGCCTCTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGAGTTTCACTgattacggatt                                                    <  2:315673/149‑1 (MQ=255)
                                                                                              ttAAATCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGAGTTTCACTGATTACGGATTACGt                                                >  1:383332/1‑149 (MQ=255)
                                                                                                 taTCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTATCGAAACAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGAGTTCCCTTTTGAGGTTGATGTGCAGTTACCGAGTTTCACTTATTACGGATTACGTgcg                                             >  1:77623/2‑149 (MQ=255)
                                                                                                  aTCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTACCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGAGTTTCACTGATTACGGATTACGTgcgc                                            <  2:54140/149‑1 (MQ=255)
                                                                                                     cTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGAGTTTCACTGATTACGGATTACGTGCGCTGa                                         <  1:704450/149‑1 (MQ=255)
                                                                                                      tCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGAGTTTCACTGATTACGGATTACGTGCGCTGAt                                        <  2:242815/149‑1 (MQ=255)
                                                                                                      tCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGAGTTTCACTGATTACGGATTACGTGCGCTGAt                                        >  2:1016708/1‑149 (MQ=255)
                                                                                                      tCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGAGTTTCACTGATTACGGATTACGTGCGCTGAt                                        >  1:250813/1‑149 (MQ=255)
                                                                                                      tCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGAGTTTCACTGATTACGGATTACGTGCGCTGAt                                        >  1:254671/1‑149 (MQ=255)
                                                                                                      acGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCTACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCTATTTACCTTTTGAGGTTGATGTGCAGTTAACGAGTTTCACTCATTACGAATTACGTGCCATGAt                                        >  2:356370/2‑149 (MQ=255)
                                                                                                       cGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGAGTTTCACTGATTACGGATTACGTGCGCTGATc                                       <  2:800121/149‑1 (MQ=255)
                                                                                                        gCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCAGCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGAGTTTCACTGATTACGGATTACGTGCGCTGATCt                                      >  1:269149/1‑149 (MQ=255)
                                                                                                         cTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGAGTTTCACTGATTACGGATTACGTGCGCTGATCTa                                     <  2:639890/149‑1 (MQ=255)
                                                                                                         cTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCAGATTTCCCTATTGAGGTTGATGTGCAGTTAACGAGTTTCACTAATTACGGATTACGTGCGCTGATCTa                                     >  2:504992/1‑149 (MQ=255)
                                                                                                          ttttatCCTTCCCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTAGTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCTGTTAACGAGTTTCACTGATTACGGATTACGTGCGCTGATCTAc                                    >  2:614562/1‑149 (MQ=255)
                                                                                                            ttttCCTTCCCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGAGTTTCACTGATTACGGATTACGTGCGCTGATCTACAt                                  >  1:866024/1‑149 (MQ=255)
                                                                                                             tttCCTTCCCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGAGTTTCACTGATTACGGATTACGTGCGCTGATCTACATg                                 <  2:471099/149‑1 (MQ=255)
                                                                                                             tttCCTTCCACGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGAGTTTCACTGATTACGTAATACGTGAGCTGATCTACATg                                 >  2:570886/1‑149 (MQ=255)
                                                                                                               tccttccCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCATTTTGAGGTTGATGTGCAGTTAACGAGTTTCACTGATTACGGATTACGTGCGCTGATCTACATGGc                               >  2:767626/1‑149 (MQ=255)
                                                                                                               tccttccCAGAACTGAATTAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCGTTTGAGGTTGATGTGCAGGTAACGAGTTTCACTGATTACGGATTACGGGCGCTGATCTACATGGc                               <  1:583603/149‑1 (MQ=255)
                                                                                                                    ccccGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGAGTTTCACTGATTACGGATTACGTGCGCTGATCTACATGGCGTCAt                          >  1:150909/1‑149 (MQ=255)
                                                                                                                     cccGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTATGATCAATATAATTGTATTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGAGTTTCACTGATTACGGATTACGTGCGCTGATCTACATGGCGTCAtt                         <  1:104609/149‑1 (MQ=255)
                                                                                                                     cccGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAACTGTATTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGAGTTTCACTGATTACGGATTACGTGCGCTGATCTACATGGCGTCAtt                         <  2:228621/149‑1 (MQ=255)
                                                                                                                      ccGAACTGAAATAAATTAGCGACACAGAATGTGGCTGGTTTATCATAAATATAAATGTATTTTTTCCCGAGTTCCAGTTTGATGTTGATGTGCAGTTAACTAGTTTCACTGATTACTGCTTACGAGAGCTGATCTACATGGCTTCATTg                        >  2:67415/1‑149 (MQ=255)
                                                                                                                       cGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGAGTTTCACTGATTACGGATTACGTGCGCTGATCTACATGGCGTCATTGc                       <  2:250813/149‑1 (MQ=255)
                                                                                                                       cGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGAGTTTCACTGATTACGGATTACGTGCGCTGATCTACATGGCGTCATTGc                       <  2:254671/149‑1 (MQ=255)
                                                                                                                       cGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGAGTTTCACTGATTACGGATTACGTGCGCTGATCTACATGGCGTCATTGc                       <  1:980337/149‑1 (MQ=255)
                                                                                                                       cGAACTGAAATAAAGTAGCGACACAGCCTGTCGCTGGTTTATCATCAATATAACTGTATTTTTTCCCGAATTCCCTTTTGAGGTTGATGTGCAGTAAACGAGTTGCACTGATTACGGATTACGTGCGCTGATCTACATGGCGTCATTGc                       <  1:566414/149‑1 (MQ=255)
                                                                                                                         aaCTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGAGTTTCACTGATTACGGATTACGTGCGCTGATCTACATGGCGTCATTGCCa                     <  2:1005945/149‑1 (MQ=255)
                                                                                                                            tGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGAGTTTCACTGATTACGGATTACGTGCGCTGATCTACATGGCGTCATTGCCAGaa                  <  1:317324/149‑1 (MQ=255)
                                                                                                                            tGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGAGTTTCACTGATTACGGATTACGTGCGCTGATCTACATGGCGTCATTGCCAGaa                  >  1:618931/1‑149 (MQ=255)
                                                                                                                              aaataaatTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATTAATGTATTTTTTCCCGATTTCCATTTTGAGGTTGATGTGCAGTTAACGAGTTTCACTGATTACGGATTACGTGCGATGATATACATGTCGTCATTGCCAGaatg                >  1:676956/1‑147 (MQ=255)
                                                                                                                              aaataaatTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGAGTTTCACTGATTACGGATTACGTGCGCTGATCTACATGGCGTCATTGCCAGAAgg                >  1:521066/1‑149 (MQ=255)
                                                                                                                               aataaatTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGAGTTTCACTGATTACGGATTACGTGAGCTGATCTACATGGCGTCATTGCCAGAAggg               >  1:625951/1‑149 (MQ=255)
                                                                                                                                ataaatTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGAGTTTCACTGATTACGGATTACGTGCGCTGATCTACATGGCGTCATTGCCAGAAGGGc              <  1:1016708/149‑1 (MQ=255)
                                                                                                                                ataaatTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGAGTTTCACTGATTACGGATTACGTGCGCTGATCTACATGGCGTCATTGCCAGAAGGGc              >  2:14798/1‑149 (MQ=255)
                                                                                                                                ataaatTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTATCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGAGTTTCACTGATTACGTATTACTTGCCCTGATCTACATGGCGTCATTGCCAGAAGGGc              >  1:758529/1‑149 (MQ=255)
                                                                                                                                 taaatTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGAGTTTCACTGATTATGGATTACGTGCGCTGATCTACATGGCGTCATTGCCAGAAGGGCg             <  1:239547/149‑1 (MQ=255)
                                                                                                                                 taaatTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGAGTTTCACTGATTACGAATTACGTGCGCTGATCTACATGGCGTCATTGCCAGAAGGGCg             >  2:501461/1‑149 (MQ=255)
                                                                                                                                        gCGACACAGCCTGTGGCTGGTTTATCATCAGTATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGAGTTTCACTGATTACGGATTACGTGCGCTGATCTACATGGCGTCATTGCCAGAAGGGCGGATGAcc      >  1:299271/1‑149 (MQ=255)
                                                                                                                                         cGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGAGTTTCCCTGATTACGGATTACGTGCGCTGATCTACATGGCGTCATTGACAGAAGGGCGGATGACCa     >  1:770798/1‑149 (MQ=255)
                                                                                                                                         cGACACAGACTGTGGCTTGTTTATCAACAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGAGTTTCACTGATTACGGATTACGTGCGCTGATCTACATGGCGTCATTGCCAGAAGGGCGGATGACCa     <  2:562835/149‑1 (MQ=255)
                                                                                                                                           actcaGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCGCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGAGTTTCACTGATTACGGATTACGTGCGCTGTTCTACATGGCGTCATTGCCAGAAGGGCGGATGACCAGt   <  2:355724/146‑1 (MQ=255)
                                                                                                                                           aataaGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGAGTTTCACTGATTACGGATTACGTGCGCTGATCTACATGGCGTCATTGCCAGAAGGGCGGATGACCAGt   <  2:668952/145‑1 (MQ=255)
                                                                                                                                            cacaGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTGGATGTGCAGTTAACGAGTTTCACTGATTACGGATTACGTGCGATGATCTACATTGCGTCATTGCCAGAAGGGCGGATGACCAGTa  >  2:638149/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                              
TAATTCTGGTACGCCTGGCAGATATTTTGCCTGCCGGGCGAACAGTGTGATACATTGCTGTGTCGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCTTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGAGTTTCACTGATTACGGATTACGTGCGCTGATCTACATGGCGTCATTGCCAGAAGGGCGGATGACCAGTA  >  NC_000913/4405983‑4406271

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: