BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
MC JC	NC_000913	1,299,499	Δ1,199 bp	intergenic (+254/‑485)	ychE → / → oppA	UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein

Missing coverage evidence...
			seq id	start	end	size	←reads	reads→	gene	description
*	*	÷	NC_000913	1299499–1300697	1300697	1–1199	124 [0]	[0] 124	ychE/oppA	UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	= 1299498	0 (0.000)	122 (1.640)	87/288	0.0	100%	intergenic (+253/‑1684)	ychE/oppA	UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein
*	?	NC_000913	1300698 =	0 (0.000)	122 (1.640)	87/288	0.0	100%	intergenic (+1453/‑484)	ychE/oppA	UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein

Only 100 of 124 total aligned reads displayed.

CTGGGGATAACGCCTTAAATGGCGAAGAAACGGTCTAAATAGGCTGATTCAAGGCATTTACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCCTAAATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCTATAGATTCTGATACGGTCAATA  >  NC_000913/1300551‑1300845

tctcaaattacagttagatataaggatttccttaactgcttctcctcacaatcaagttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgactttggtagaggaaagtgctaaat                                                                                                                                                    >  2:417150‑M2/148‑149 (MQ=255)

 ctcaaattacagttagttataaggatttccttaactgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaattgctaaata                                                                                                                                                   >  2:992927‑M2/147‑149 (MQ=255)

 ctcaaattacagttagttataaggatttccttaactgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaata                                                                                                                                                   <  1:894967‑M2/3‑1 (MQ=255)

  tcaaattacagttagttataaggatttccttaactgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaataa                                                                                                                                                  >  2:133301‑M2/146‑149 (MQ=255)

  tcaaattacagttagttataaggatttccttaactgcttctactcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataatttaaatgatattattaattacactgccattggtagaggaaagtgctaaataa                                                                                                                                                  >  1:1068681‑M2/146‑149 (MQ=255)

  tcaaattacagttagttataaggatttccttaactgcttatccacaccatcatgttattttcgacacatcataatcctgtgctgtctgaagaataattgaaaagatattattaattccactgcctttggtagatgaaagtgctaaataa                                                                                                                                                  >  1:1073650‑M2/146‑149 (MQ=255)

    aaattacagttagttataaggatttccttaactgcttctcctcaccatcatgttattttcgccacatcatactcctgcgcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaataata                                                                                                                                                <  2:1137117‑M2/6‑1 (MQ=255)

    aaattacagttagttataaggatttccttaactgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaataata                                                                                                                                                >  2:59426‑M2/144‑149 (MQ=255)

    aaattacagttagttataaggatttcctaaactgcttctcctaaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaataata                                                                                                                                                >  2:966716‑M2/144‑149 (MQ=255)

      attacagttagttataaggatttccttaactgcttctcctcaccatcatgttattttcgccacatcataatcctgtgcttgctgaagaataattgaaatgaaattattaattccactgcctttggtagaggaaagtgctaaataataat                                                                                                                                              <  1:818577‑M2/8‑1 (MQ=255)

      attacagttagttataaggatttccttaactgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaataataat                                                                                                                                              <  1:301456‑M2/8‑1 (MQ=255)

             ttagttataaggatttccttaactgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGt                                                                                                                                       >  2:441022‑M2/135‑149 (MQ=255)

               agttataaggatttccttaactgcttctcctaaccatcatgttattttcgccacatcataatcctgggctttctgaagaataattgaaatgatattattaattccactgaatttggtagaggaaagtgctaaATAATAATCAATTGTTa                                                                                                                                     <  2:354137‑M2/17‑1 (MQ=255)

                tttataaggatttccttaactgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTaa                                                                                                                                    <  1:627332‑M2/18‑1 (MQ=255)

                gttataaggatttccttaactgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTaa                                                                                                                                    >  2:1053851‑M2/132‑149 (MQ=255)

                gttataaggatttccttaactgcttctcctcaccatcatgttattttagccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattacactgcatttggtagaggaaagtgctaaATAATAATCAATTGTTaa                                                                                                                                    >  2:928919‑M2/132‑149 (MQ=255)

                 ttataaggatttccttaactgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTaaa                                                                                                                                   <  2:521648‑M2/19‑1 (MQ=255)

                    taaggatttccttaactgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAatta                                                                                                                                >  1:522898‑M2/128‑149 (MQ=255)

                     aaggatttccttaactgcttctcctcaccatgatgttattttcgccgcataataatcctgggcttgctgaagaataattgaaatgatattattaattcacctgcgattggtagaggaaagtccgaaATAATAATCAATTGGtaaatatt                                                                                                                               >  2:1151608‑M2/127‑146 (MQ=255)

                      agtatttccttaactgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAattatt                                                                                                                              <  1:936747‑M2/24‑1 (MQ=255)

                       ggatttccttaactgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATtg                                                                                                                             >  2:269426‑M2/125‑149 (MQ=255)

                         atttccttaactgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATtgtg                                                                                                                           <  2:844038‑M2/27‑1 (MQ=255)

                         atttccttaactgcttctcctcaccatcatgatattttcgccacatcataatcctggccttgctgaagaataattgaaatgatattattaattccactgcctttcgtagaggaaagtgcgaaATAATAATCAAATGTtaaattattttt                                                                                                                           >  1:238179‑M2/123‑146 (MQ=255)

                           ttccttaactgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCa                                                                                                                         >  1:701829‑M2/121‑149 (MQ=255)

                              cttaactgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCAttt                                                                                                                      >  2:601117‑M2/118‑149 (MQ=255)

                              cttaactgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCAttt                                                                                                                      >  2:763092‑M2/118‑149 (MQ=255)

                              cttaactgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCAttt                                                                                                                      <  1:17784‑M2/32‑1 (MQ=255)

                              cttaactgcttctcctcaccataattttattttcgacacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCAttt                                                                                                                      <  1:16105‑M2/32‑1 (MQ=255)

                               ttaactgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTc                                                                                                                     >  1:919971‑M2/117‑149 (MQ=255)

                                taactgctgctcctcaccatcatgttattttcgccacatcaaaatcctgggcgtgctgaagaataattgtaatgatattattaattccactgcctttagtagggtaaagtgctaaATAATAATCATTTGTTAAATTATTGTGCATTTCa                                                                                                                    <  2:415985‑M2/34‑1 (MQ=255)

                                   ctgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCacta                                                                                                                 >  2:285738‑M2/113‑149 (MQ=255)

                                    tgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcatttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCactac                                                                                                                >  2:34926‑M2/112‑149 (MQ=255)

                                     tcttctcctcaacatcatgttattttctccaaatcataatccttggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCactact                                                                                                               <  1:545172‑M2/39‑1 (MQ=255)

                                     gcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCactact                                                                                                               <  2:1020091‑M2/39‑1 (MQ=255)

                                     gcttctcctcacaatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCactact                                                                                                               <  2:1097287‑M2/39‑1 (MQ=255)

                                      cttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTg                                                                                                              <  1:134825‑M2/40‑1 (MQ=255)

                                       ttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTgg                                                                                                             >  1:859564‑M2/109‑149 (MQ=255)

                                       ttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTgg                                                                                                             >  1:235875‑M2/109‑149 (MQ=255)

                                         ctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGaa                                                                                                           <  1:59426‑M2/43‑1 (MQ=255)

                                           cctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACt                                                                                                         <  1:518294‑M2/45‑1 (MQ=255)

                                            ctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTg                                                                                                        >  1:390778‑M2/104‑149 (MQ=255)

                                            ctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTg                                                                                                        >  2:439754‑M2/104‑149 (MQ=255)

                                             tcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGt                                                                                                       >  2:1135734‑M2/103‑149 (MQ=255)

                                                 catcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATc                                                                                                   <  2:522898‑M2/51‑1 (MQ=255)

                                                 cataatgttattttcgccacatcataatcctggtcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctatATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATc                                                                                                   <  1:748975‑M2/51‑1 (MQ=255)

                                                      tgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGaaa                                                                                              <  1:1167274‑M2/56‑1 (MQ=255)

                                                       gttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGaaaa                                                                                             <  2:123224‑M2/57‑1 (MQ=255)

                                                           ttttagccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATa                                                                                         <  1:190352‑M2/61‑1 (MQ=255)

                                                                gccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGAATTTCACTACTGGAACTGTAATCAGAAAAGATAGACAt                                                                                    >  2:1234577‑M2/84‑149 (MQ=255)

                                                                 ccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccacagcctttggttgaggaaagtgcgaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGCCATg                                                                                   >  2:717081‑M2/83‑149 (MQ=255)

                                                                      tcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAg                                                                              <  1:992927‑M2/72‑1 (MQ=255)

                                                                       cataatcctgggcttgctgaagaataaatgaaatcatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGAAATCAGAAAAGATAGACATGCTTAGc                                                                             <  2:1152070‑M2/73‑1 (MQ=255)

                                                                            tcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAAtc                                                                        >  1:302288‑M2/72‑149 (MQ=255)

                                                                               tgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATctct                                                                     >  2:1218833‑M2/69‑149 (MQ=255)

                                                                                     tgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATttga                                                               <  2:784198‑M2/87‑1 (MQ=255)

                                                                                     tgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATttga                                                               <  2:928490‑M2/87‑1 (MQ=255)

                                                                                     tgctgaagaataattgaaatgatattattaattacactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATttga                                                               <  1:522446‑M2/87‑1 (MQ=255)

                                                                                          aagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGattgaa                                                          >  1:56286‑M2/58‑149 (MQ=255)

                                                                                           ataataattgaaatgatattattaattccactgcctttggtagagtaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGattgaat                                                         <  1:1210535‑M2/93‑1 (MQ=255)

                                                                                           agaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGattgaat                                                         >  1:735629‑M2/57‑149 (MQ=255)

                                                                                            gaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGattgaatt                                                        <  1:269426‑M2/94‑1 (MQ=255)

                                                                                              ataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGattgaattga                                                      >  2:266086‑M2/54‑149 (MQ=255)

                                                                                                aattgaaatgatattattaattccactgcctttggtagatgaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGaaa                                                    <  2:701829‑M2/98‑1 (MQ=255)

                                                                                                aattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGaaa                                                    <  2:390778‑M2/98‑1 (MQ=255)

                                                                                                      aatgatattattaattccacttccttttgtagaggaaagtgctaaATAATAATCAATTGTTATATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATCGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGAtgtt                                              <  2:671482‑M2/104‑1 (MQ=255)

                                                                                                      aatgaaattattaattccactgcctttggtattcgaaagtgctaaATAATAATCATTTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGAtgtt                                              <  1:1151608‑M2/104‑1 (MQ=255)

                                                                                                       attatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGAtgttt                                             <  1:100438‑M2/105‑1 (MQ=255)

                                                                                                       attatattattaattcaactgactttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTTCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTATATTTGATTGAATTGAAAGAtgttt                                             <  1:354701‑M2/105‑1 (MQ=255)

                                                                                                       atgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGAtgttt                                             <  1:601117‑M2/105‑1 (MQ=255)

                                                                                                       atgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGAtgttt                                             <  1:937904‑M2/105‑1 (MQ=255)

                                                                                                          atattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGAtgtttgtt                                          >  1:993107‑M2/42‑149 (MQ=255)

                                                                                                            attattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGAtgtttgtt                                          >  1:759409‑M2/40‑147 (MQ=255)

                                                                                                            attattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGAtgtttgtt                                          <  2:759409‑M2/108‑1 (MQ=255)

                                                                                                             ttattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAg                                       >  1:385437‑M2/39‑149 (MQ=255)

                                                                                                             ttattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAg                                       >  2:1034551‑M2/39‑149 (MQ=255)

                                                                                                              tattaattccactgccgttggtagaggaaagtgctaaATAATAATCAATTGTTTAATTATTGTGCATGTCACTACTGGAACTGGAATCAGAACAGATAGACATGCTAAGCCAATCTCTATTAGATTGAATTGAAAGATGTTTGTTTAgg                                      >  2:904103‑M2/38‑149 (MQ=255)

                                                                                                               tttatttgcactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGc                                     <  1:928919‑M2/113‑1 (MQ=255)

                                                                                                                ttaattccactgccttttgtagtggtaagtgctaaATAATATTCTATTGTTATATTATTGTGCATTTCACTACTGGAAGTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCa                                    <  1:1057806‑M2/114‑1 (MQ=255)

                                                                                                                 taattacacttcctttgggatatgaaattgctacATTATAATCAATTTTTAAATTATTTTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTATCCAATCTATATTTGATTGAATTGAAAGATGTTTGTTAAGGCAt                                   <  2:1068681‑M2/115‑1 (MQ=255)

                                                                                                                  aattcgtctgcctttggtagaggaaagtgctaaATAATAATCTATTGTTAAATTATTGTGAATTTCACTACTGGAACTGTAATCAGAAAAGATAGACTTGCTTAGCCAATCTCTTTTTGATTTAATTGAAAGATGTTTGTTAAGTCATg                                  <  2:97231‑M2/116‑1 (MQ=255)

                                                                                                                  aattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAATATGTTTGTTAAGGCATg                                  <  2:249443‑M2/116‑1 (MQ=255)

                                                                                                                  aattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATg                                  <  1:494932‑M2/116‑1 (MQ=255)

                                                                                                                    ttccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGa                                >  2:941952‑M2/32‑149 (MQ=255)

                                                                                                                     tccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGAt                               <  1:133301‑M2/119‑1 (MQ=255)

                                                                                                                     tccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGAt                               >  2:158625‑M2/31‑149 (MQ=255)

                                                                                                                       cactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGc                             >  1:870092‑M2/29‑149 (MQ=255)

                                                                                                                        actgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTTAATTGAAAGATGTTTGTTAAGGCATGGATGCa                            >  2:1231595‑M2/28‑149 (MQ=255)

                                                                                                                        actgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCa                            >  2:124546‑M2/28‑149 (MQ=255)

                                                                                                                             ctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCta                       <  2:311943‑M2/127‑1 (MQ=255)

                                                                                                                                 ggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCTATAGa                   >  2:346311‑M2/19‑149 (MQ=255)

                                                                                                                                   tagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCaa                           <  1:998911‑M2/123‑1 (MQ=255)

                                                                                                                                     gaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCTATAGATTCt               >  2:848098‑M2/15‑149 (MQ=255)

                                                                                                                                      aggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCTATAGATTCTg              >  1:318290‑M2/14‑149 (MQ=255)

                                                                                                                                      aggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCTATAGATTCTg              >  1:989856‑M2/14‑149 (MQ=255)

                                                                                                                                       ggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCTATAGATTCTGa             <  2:913207‑M2/137‑1 (MQ=255)

                                                                                                                                         aaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGACGCAAGCTATAGATTCTGATa           <  1:1207967‑M2/139‑1 (MQ=255)

                                                                                                                                           agtgctaaATAATAATAAATTGTTAAATTTTTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCTATAGATTCTGATACg         <  1:34926‑M2/141‑1 (MQ=255)

                                                                                                                                             tgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCTATAGATTCTGATACGGt       <  1:1053851‑M2/143‑1 (MQ=255)

                                                                                                                                                 aaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCTATAGATTCTGATACGGTCAAt   >  2:187001‑M2/3‑149 (MQ=255)

                                                                                                                                                  aATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCTATAGATTCTGATACGGTCAATa  >  1:307412‑M2/2‑149 (MQ=255)

CTGGGGATAACGCCTTAAATGGCGAAGAAACGGTCTAAATAGGCTGATTCAAGGCATTTACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCCTAAATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCTATAGATTCTGATACGGTCAATA  >  NC_000913/1300551‑1300845

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`