Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 619,410 T→C 10.5% T264A (ACG→GCG)  fepC ← ferrienterobactin ABC transporter ATPase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913619,4100TC10.5% 469.5 / 33.3 162T264A (ACG→GCG) fepCferrienterobactin ABC transporter ATPase
Reads supporting (aligned to +/- strand):  ref base T (84/60);  new base C (8/9);  total (92/70)
Fisher's exact test for biased strand distribution p-value = 4.41e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 163 total aligned reads displayed.

GGGTAAGGCGATTATTGTGATCCTTTCCGCGTTGATCGGCGGGATGGTGGCTTGTGGTGGCGTGCTGTTGCGCTATGCGATGGCATCCAGAAAACAGGATGCCATGATGGCAGACCACTTAGTTTAACTATTTGCGGTTGAAGGTGCCGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCAACTGGATCGTCAATGATCATGCAGCGCAGACCATAAATGCGCTCAATCAGTTCAGCAGTGACAATCTCCTTCGGCGCTCCCTGAGCAACAATTTTCCCTTCCCGCAATGCAATC  >  NC_000913/619261‑619558
                                                                                                                                                     |                                                                                                                                                    
  gTAAGGCGATTATTGTGATCCTTTCCGCGTTGATCGGCGGGATGGTGGCTTGTGGTGGCGTGCTGTTGCGCTATGCGATGGCATCCAGAAAACAGGATGCCATGATGGCAGACCACTTAGTTTAACTATTTGCGGTTGAAGGTGCCGTTc                                                                                                                                                    >  1:947861/1‑150 (MQ=255)
       gCGATTATTGTGATCCTTTCCGCGTTGATCGGCGGGATGGTGGCTTGTGGTGGCGTGCTGTTGCGCTATGCGATGGCATCCAGAAAACAGGATGCCATGATGGCAGACCACTTAGTTTAACTATTTGCGGTTGAAGGTGCCGTTCGTCCg                                                                                                                                               >  2:1061954/1‑150 (MQ=255)
       gCGATTATTGTGATCCTTTCCGCGTTGATCGGCGGGATGGTGGCTTGTGGTGGCGTGCTGTTGCGCTATGCGATGGCATCCAGAAAACAGGATGCCATGATGGCAGACCACTTAGTTTAACTATTTGCGGTTGAAGGTGCCGTTCGTCCg                                                                                                                                               >  1:2302109/1‑150 (MQ=255)
         gATTTTTGTGATCCTTTCCGCGTTGATGGGCGGGATGGTGGCTTGTTGTGGCGTTCTGTTTGGCTATTCGATGGCATACAGAAATCAGTATTCCATGTTTGCATACCACTTATTTTAACTATTTGCGGTTGAAGGTGCCGTTCGTCGGAg                                                                                                                                             <  2:656122/150‑1 (MQ=255)
            tattGTGATCCTTTCCGCGTTGATCGGCGGGATGGTGGCTTGTGGTGGCGTGCTGTTGCGCTATGCGATGGCATCCAGAAAACAGGATGCCATGATGGCAGACCACTTAGTTTAACTATTTGCGGTTGAAGGTGCCGTTCGTCCGAGCgg                                                                                                                                          <  1:1573125/150‑1 (MQ=255)
             attGTGATCCGTTCCGCGTTGATCGGCGGGATGGTGGCTTGTGGTGGCGTGCTGTTGCGCTATGCGATGGCATCCAGAAAACAGGATGCCATGATGGCAGACCACTTAGTTTAACTATTTGCGGTTGAAGGTGCCGCTCGGACGAACGGc                                                                                                                                         <  1:1091345/150‑1 (MQ=255)
               tgtgATCCTTTCCGCGTTGATCGGCGGGATGGTGGCTTGTGGTGGCGTGCTGTTGCGCTATGCGATGGCATCCAGAAAACAGGATGCCATGATGGCAGACCACTTAGTTTAACTATTTGCGGTTGAAGGTGCCGCTCGGACGAACGGcac                                                                                                                                       <  2:2446067/150‑1 (MQ=255)
                 tgATCCTTTCCGCGTTGATCGGCGGGATGGTGGCTTGTGGTGGCGTGCTGTTGCGCTATGCGATGGCATCCAGAAAACAGGATGCCATGATGGCAGACCACTTAGTTTAACTATTTGCGGTTGAAGGTGCCGTTCGTCCGAGCGGcacca                                                                                                                                     >  1:665467/1‑150 (MQ=255)
                    tCCTTTCCGCGTTGATCGGCGGGATGGTGGCTTGTGGTGGCGTGCTGTTGCGCTATGCGATGGCATCCAGAAAACAGGATGCCATGATGGCAGACCACTTAGTTTAACTATTTGCGGTTGAAGGTGCCGTTCGTCCGAGCGGCACCACaa                                                                                                                                  >  2:2089347/1‑150 (MQ=255)
                     ccTGTCCGCGTTGATCGGCGGGATGGTGGCTTGTGGTGGCGTGCTGTTGCGCTATGCGATGGCATCCAGAAAACAGGATGCCATGATGGCAGACCACTTAGTTTAACTATTTGCGGTTGAAGGTGCCGCTCGGACGAACGGcaccttcaa                                                                                                                                 <  2:1604438/150‑6 (MQ=255)
                       tttCCGCGTTGATCGGCGGGATGGTGGCTTGTGGTGGCGTGCTGTTGCGCTATGCGATGGCATCCAGAAAACAGGATGCCATGATGGCAGACCACTTAGTTTAACTATTTGCGGTTGAAGGTGCCGTTCGTCCGAGCGGCACCACAAgcg                                                                                                                               <  1:1641371/150‑1 (MQ=255)
                            gcgTTGATCGGCGGGATGGTGGCTTGTGGTGGCGTGCTGTTGCGCTATGCGATGGCATCCAGAAAACAGGATGCCATGATGGCAGACCACTTAGTTTAACTATTTGCGGTTGAAGGTGCCGTTCGTCCGAGCGGCACCACAAGCGGCGtt                                                                                                                          >  2:720107/1‑150 (MQ=255)
                            gcgTTCATCCGCGGGATGGTGGCTTGTGGTGGCGTGCTGTTGCGCTATGCGATGGCATCCAGAAAACAGGATGCCATGATGGCAGACCACTTAGTTTAACTATTTGCGGTTGAAGGTGCCGTTCGTCCGAGCGGCACCACAAGCGGCGtt                                                                                                                          >  1:1842048/1‑150 (MQ=255)
                              gTTGATCGGCGGGATGGTGGCTTGTGGTGGCGTGCTGTTGCGCTATGCGATGGCATCCAGAAAACAGGATGCCATGATGGCAGACCACTTAGTTTAACTATTTGCGGTTGAAGGTGCCGTTCGTCCGAGCGGCACCACAAGCGGCGTTcc                                                                                                                        >  1:1686715/1‑150 (MQ=255)
                              gTTGATCGGCGGGATGGTGGCTTGTGGTGGCGTGCTGTTGCGCTATGCGATGGCATCCAGAAAACAGGATGCCATGATGGCAGACAACTTAGTTTATCTATTTGCGGTTGAAGGTGCCGTTCGTCCGAGCGGCACCACAAGCGGCGTTcc                                                                                                                        <  2:2631868/150‑1 (MQ=255)
                               ttGATCGGCGGGATGGTGGCTTGTGGTGGCGTGCTGTTGCGCTATGCGATGGCATCCAGAAAACAGGATGCCATGATGGCAGACCACTGAGTTTAACTATTTGCGGTTGAAGGTGCCGTTCGTCCGAGCGGCACCACCAGCGGCGTTCCg                                                                                                                       >  2:3080371/1‑150 (MQ=255)
                                tGATCGGCGGGATGGTGGCTTGTGGTGGCGTGCTGTTGCGCTATGCGATGGCATCCAGAAAACAGGATGCCATGATGGCAGACCACTTTGTTTAACTTTTTGCGGTTGAAGGTGCCGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCgg                                                                                                                      <  1:2881184/150‑1 (MQ=255)
                                tGAGCGGCGGGTTGGTGGGTTGTGGTGGCGTGCTGTTGCGCTATGCGATGGCATCCATAAAACAGGATGCCTTGATGGCAGACCACTTAGTTTAACTATTTGCGGTTGAAGGTGCCGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCgg                                                                                                                      <  1:2881246/150‑1 (MQ=255)
                                  aTCGGCGGGATGGTGGCTTGTGGTGGCGTGCTGTTGCGCTATGCGATGGCATCCAGAAAACAGGATGCCATGATGGCAGACCACTTAGTTTAACTATTTGCGGTTGAAGGTGCCGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCa                                                                                                                    >  1:954887/1‑150 (MQ=255)
                                      gcggGATGGTGGCTTGTGGTGGCGTGCTGTTGCGCTATGCGATGGCATCCAGAAAACAGGATGCCATGATGGCAGACCACTTAGTTTAACTATTTGCGGTTGAAGGTGCCGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCAACTg                                                                                                                >  1:577814/1‑150 (MQ=255)
                                       cggGATGGTGGCTTGTGGTGGCGTGCTGTTGCGCTATGCGATGGCATCCAGAAAACAGGATGCCATGATGGCAGACCACTTAGTTTAACTTTTTGCGGTTGAAGGTGCCGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCAACTgg                                                                                                               <  1:1240661/150‑1 (MQ=255)
                                         ggATGGTGGCTTGTGGTGGCGTGCTGTTGCGCTATGCGATGGCATCCAGAAAACAGGATGCCATGATGGCAGACCACTTAGTTTAACTATTTGCGGTTGAAGGTGCCGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCAACTGGAt                                                                                                             <  1:2558924/150‑1 (MQ=255)
                                          gttggtggCTTGTGGTGGCGTGCTGTTGCGCTATGCGATGGCATCCAGAAAACAGGATGCCATGATGGCAGACCACTTAGTTTAACTATTTGCGGTTGAAGGTGCCGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCAACTGGATc                                                                                                            <  1:1166337/148‑1 (MQ=255)
                                          gctggtggCTTGTGGTGGCGTGCTGTTGCGCTATGCGATGGCATCCAGAAAACAGGATGCCATGATGGCAGACCACTTAGTTTAACTATTTGCGGTTGAAGGTGCCGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCAACTGGATc                                                                                                            >  1:3062402/3‑150 (MQ=255)
                                          gATGGTGGCTTGTGGTGGCGTGCTGTTGCGCTATGCGATGGCATCCAGAAAACAGGATGCCATGATGGCAGACCACTTAGTTTAACTATTTGCGGTTGAAGGTGCCGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCAACTGGATc                                                                                                            <  2:275002/150‑1 (MQ=255)
                                           aTGGTGGCTTGTGGTGGCGTGCTGTTGCGCTATGCGATGGCATCCAGAAAACAGGATGCCATGATGGCAGACCACTTAGTTTAACTATTTGCGGTTGAAGGTGCCGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCAACTGGATCg                                                                                                           >  2:455278/1‑150 (MQ=255)
                                                     gtggtgGCGTGCTGTTGCGCTATGCGATGGCATCCAGAAAACAGGATGCCATGATGGCAGACCACTTAGTTTAACTATTTGCGGTTGAAGGTGCCGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCACCTGGATCGTCAATGATCa                                                                                                 >  1:1235783/1‑150 (MQ=255)
                                                      tggtggCGTGCTGTTGCGCTATGCGATGGCATCCAGAAAACAGGATGCCATGATGGCAGACCACTTAGTTTCACGATTTGCGGTTGAAGGTGCCGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCAACTGGATCGTCAATGATCag                                                                                                >  2:3067377/1‑149 (MQ=255)
                                                      tggtggCGTGCTGTTGCGCTATGCGATGGCATCCAGAAAACAGGATGCCATGATGGCAGACCACTTAGTTTAACTATTTGCGGTTGAAGGTGCCGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCAACTGGATCGTCAATGATCAt                                                                                                >  1:1577578/1‑150 (MQ=255)
                                                      tggtggCGTGCTGTTGCGCTATGCGATGGCATCCAGAAAACAGGATGCCATGATGGCAGACCACTTAGTTTAACTATTTGCGGTTGAAGGTGCCGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCAACTGGATCGTCAATGATCAt                                                                                                >  1:1217613/1‑150 (MQ=255)
                                                      tggtggCGTGCTGTTGCGCTATGCGATGGCATCCAGAAAACAGGATGCCATGATGGCAGACCACTTAGTTTAACTATTTGCGGTTGAAGGTGCCGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCAACTGGATCGTCAATGATCAt                                                                                                >  1:2763879/1‑150 (MQ=255)
                                                      tggtggCGTGCTGTTGCGCTATGCGATGGCATCCAGAAAACAGGATGCCATGATGGCAGACCACTTAGTTTAACTATTTGCGGTTGAAGGTGCCGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCAACTGGATCGTCAATGATCAt                                                                                                >  2:711122/1‑150 (MQ=255)
                                                      tggtggCGTGCTGTTGCGCTATGCGATGGCATCCAGAAAACAGGATGCCATGATGGCAGACCACTTAGTTTAACTATTTGCGGTTGAAGGTGCCGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCAACTGGATCGACAATGATCAt                                                                                                >  2:3067358/1‑150 (MQ=255)
                                                      tggaggCGTGCTGTAGCGCTATGCGATGGCATCCAGAAAACAGGAAGCCATGATGTCAGACCACATAGTTTAACTATTTGCGGTTGAAGGTGCCGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCAACTGGATCGTCAAAGATCaa                                                                                                >  2:1196241/1‑149 (MQ=255)
                                                         tggCGTGCTGTTGCGCTATGCGATGGCATCCAGAAAACAGGATGCCATGATGGCAGACCACTTAGTTTAACTATTTGCGGTTGAAGGTGCCGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCAACTGGATCGTCAATGATCATGCa                                                                                             >  1:2548038/1‑150 (MQ=255)
                                                         tggCGTGCTGTTGCGCTATGCGATGGCATCCAGAAAACAGGATGCCATGATGGCAGACCACTTAGTTTAACTATTTGCGGTTGAAGGTGCCGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCAACTGGATCGTCAATGATCATGCa                                                                                             <  2:356079/150‑1 (MQ=255)
                                                         tggCGTGCTGTTGCGCTATGCGATGGCATCCAGAAAACAGGATGCCATGATGGCAGACCACTTAGTTTAACTATTTGCGGTTGAAGGTGCCGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCAACTGGATCGTCAATGATCATGCa                                                                                             >  1:233935/1‑150 (MQ=255)
                                                          ggCGTGCTGTTGCGCTATGCGATGGCATCCAGAAAACAGGATGCCATGATGGCAGACCACTTAGTTTAACTATTTGCGGTTGAAGGTGCCGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCAACTGGATCGTCAATGATCATGCAg                                                                                            >  1:1411768/1‑150 (MQ=255)
                                                           gCGTGCTGTTGCGCTATGCGATGGCATCCAGAAAACAGGATGCCATGATGGCAGACCACTTAGTTTAACTATTTGCGGTTGAAGGTGCCGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCAACTGGATCGTCAATGATCATGCAgc                                                                                           <  2:222951/150‑1 (MQ=255)
                                                           gCGTGCTGTTGCGCTATGCGATGGCATCCAGAAAACAGGATGCCATGATGGCAGACCACTTAGTTTAACTATTTGCGGTTGAAGGTGCCGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCAACTGGATCGTCAATGATCATGCAgc                                                                                           <  2:2329400/150‑1 (MQ=255)
                                                              ttcTGTTGCGCTTTGCGATGGCATCCAGATAACTGGATTCTTTGATGGCAGACCACTTAGTTTAACTATTTGCGGTTGATGGTGCCTTTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCAACTGGATCGTCATTGATCATGCAGCGCa                                                                                        <  1:1138485/148‑1 (MQ=255)
                                                              tGCTGTTGCGCTATGCGATGGCATCCAGAAAACAGGATGCCATGATGGCAGACCACTTAGTTTAACTATTTGCGGTTGAAGGTGCCGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCAACTGGATCGTCAATGATCATGCAGCGCa                                                                                        <  2:2354298/150‑1 (MQ=255)
                                                                     gcgcTATGCGATGGCATCCAGAAAACAGGATGCCATGATGGCAGACCACTTAGTTTAACTATTTGCGGTTGAAGGTGCCGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCAACTGGATCGTCCATGATCATGCAGCGCAGACCATa                                                                                 >  1:2450220/1‑150 (MQ=255)
                                                                          aTGCGATGGCATCCAGAAAACAGGATGCCATGATGGCAGACCACTTAGTTTAACTATTTGCGGTTGAAGGTGCCGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCAACTGGATCGTCAATGATCATGCAGCGCAGACCATAAATgc                                                                            <  2:288042/150‑1 (MQ=255)
                                                                           tGCGATGGCATCCAGAAAACAGGATGCCATGATGGCAGACCACTTAGTTTAACTATTTGCGGTTGAAGGTGCCGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCAACTGGATCGTCAATGATCATGCAGCGCAGACCATAAATgcg                                                                           >  1:2492388/1‑150 (MQ=255)
                                                                           tGCGATGGCATCCAGAAAACAGGATGCCATGATGGCAGACCACTTAGTTTAACTATTTGCGGTTGAAGGTGCCGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCAACTGGATCGTCAATGATCATGCAGCGCAGACCATAAATgcg                                                                           <  2:85150/150‑1 (MQ=255)
                                                                           tGCGATGGCATCCAGAAAACAGGATGCCATGATGGCAGACCACTTAGTTTAACTATTTGCGGTTGAAGGTGCCGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCAACTGGATCGTCAATGATCATGCAGCGCAGACCAGAAATgcg                                                                           >  2:523278/1‑150 (MQ=255)
                                                                              gATGGCATCCAGAAAACAGGATGCCATGATGGCAGACCACTTAGTTTAACTATTTGCGGTTGAAGGTGCCGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCAACTGGATCGTCAATGATCATGCAGCGCAGACCATAAATGCGCTc                                                                        <  1:1967565/150‑1 (MQ=255)
                                                                                   cATCCAGAAAACAGGATGCCATGATGGCAGACCACTTAGTTTAACTATTTGCGGTTGAAGGTGCCGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCAACTGGATCGTCAATGATCATGCAGCGCAGACCATAAATGCGCTCAATCa                                                                   <  1:223967/150‑1 (MQ=255)
                                                                                          aaaaCAGGATGCCATGATGGCAGACCACTTAGTTTAACTATTTGCGGTTGAAGGTGCCGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCAACTGGATCGTCAATGATCATGCAGCGCAGACCATAAATGCGCTCAATCAGTTcagc                                                            >  1:2708114/1‑150 (MQ=255)
                                                                                          aaaaCAGGATGCCATGATGGCAGACCACTTAGTTTAACTATTTGCGGTTGAAGGTGCCGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCAACTGGATCGTAAATTATCATGCAGCGCAGACCATAAAGGCGCTCAATCAGTTaagc                                                            >  1:1997631/1‑150 (MQ=255)
                                                                                           aaaCTGGATGCCATGATGGCAGACCACTTAGTTTAACTATTTGCGGTAGAAGGTGCCGTTCGTCACAGCGGCACCACAATCGGCGTTCCGGCAACTGGATCGTCAATGATCATGCAGCGCAGACCATAAATGCGCTCAATCAGTTcagca                                                           >  2:883364/1‑150 (MQ=255)
                                                                                           aaaCAGGATGCCATGATGGCAGACCACTTAGTTTAACTATTTGCGGTTGACGGTGCCGTGCGGCCGTGCGGCACCACAAGCGGCGGTCCGGCAACTGGGTCGTCAATGATCATGCAGCGCAGACCCGACATGCGCTCAATCTGTGccgca                                                           >  2:1941165/1‑150 (MQ=255)
                                                                                           aaaCAGGATGCCATGATGGCAGACCACTTAGTTTAACTATTTGCGGTTGAAGGTGCCGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCAACTGGATCGTCAATGATCATGCAGCGCAGACCATAAATGCGCTCAATCAGTTcagca                                                           >  2:1321431/1‑150 (MQ=255)
                                                                                            aaCAGGATGCCATGATGGCAGACCACTTAGTTTAACTATTTGCGGTTGAAGGTGCCGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCAACTGGATCGTCAATGATCATGCAGCGCAGACCATAAATGCGCTCAATCAGTTcagcag                                                          <  1:2654/150‑1 (MQ=255)
                                                                                             aCAGGATGCCATGATGGCAGACCACTTAGTTTAACTATTTGCGGTTGAAGGTGCCGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCAACTGGATCGTCAATGATCATGCAGCGCAGACCAGAAATGCGCTCAATCAGTTCAGCAGt                                                         >  2:2580473/1‑150 (MQ=255)
                                                                                              cAGGATGCCATGATGGCAGACCACTTAGTTTAACTATTTGCGGTTGAAGGTGCCGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCAACTGGATCGTCAATGATCATGCAGCGCAGACCATAAATGCGCTCAATCAGTTCAGCAGTg                                                        <  2:489219/150‑1 (MQ=255)
                                                                                               aGGATGCCATGATGGCAGACCACTTAGTTTAACTATTTGCGGTTGAAGGTGCCGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCAACTGGATCGTCAATGATCATGCAGCGCAGACCATAAATGCGCTCAATCAGTTCAGCAGTGa                                                       >  2:1350677/1‑150 (MQ=255)
                                                                                                  aTGCCATGATGGCAGACCACTTAGTTTAACTATTTGCGGTTGAAGGTGCCGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCAACTGGATCGTCAATGATCATGCAGCGCAGACCATAAATGCGCTCAATCAGTTCAGCAGTGACaa                                                    <  1:1024910/150‑1 (MQ=255)
                                                                                                  aTGCCATGATGGCAGACCACTTAGTTTAACTATTTGCGGTTGAAGGTGCCGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCAACTGGATCGTCAATGATCATGCAGCGCAGACCATAAATGCGCTCAATCAGTTCAGCAGTGACaa                                                    <  2:272384/150‑1 (MQ=255)
                                                                                                      cATGATGGCAGACCACTTAGTTTAACTATTTGCGGTTGAAGGTGCCGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCAACTGGATCGTCAATGATCATGCAGCGCAGACCATAAATGCGCTCAATCAGTTCAGCAGTGACAAtctc                                                >  1:28680/1‑150 (MQ=255)
                                                                                                       atgatgGCAGACCACTTAGTTTAACTATTTGCGGTTGAAGGTGCCGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCAACTGGATCGTCAATGATCATGCAGCGCAGACCATAAATGCGCTCAATCAGTTCAGCAGTGACAATCTcc                                               >  2:2773638/1‑150 (MQ=255)
                                                                                                       atgatgGCAGACCACTTAGTTTAACTATTTGCGGTTGAAGGTGCCGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCAACTGGATCGTCAATGATCATGCAGCGCAGACCATAAATGCGCTCAATCAGTTCAGCAGTGACAATCTcc                                               <  2:577814/150‑1 (MQ=255)
                                                                                                       atgatgGCAGACCACTTAGTTTAACTATTTGCGGTTGAAGGTGCCGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCAACTGGATCGTCAATGATCATGCAGCGCAGACCATAAATGCGCTCAATCAGTTCAGCAGTGACAATCTcc                                               >  2:2773611/1‑150 (MQ=255)
                                                                                                       atgatgGCAGACCACTTAGTTTAACTATTTGCGGTTGAAGGTGCCGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCAACTGGATCGTCAATGATCATGCAGCGCAGACCATAAATGCGCTCAATCAGTTCAGCAGTGACAATCTcc                                               >  2:2497263/1‑150 (MQ=255)
                                                                                                       atgatgGCAGACCACTTAGTTTAACTATTTGCGGTTGAAGGTGCCGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCAACTGGATCGTCAATGATCATGCAGCGCAGACCATAAATGCGCTCAATCAGTTCAGCAGTGACAATCTcc                                               <  2:2494298/150‑1 (MQ=255)
                                                                                                        tgatgGCAGACCACTTAGTTTAACTATTTGCGGTTGAAGGTGCCGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCAACTGGATCGTCAATGATCATGCAGCGCAGACCATAAATGCGCTCAATCAGTTCAGCAGTGACAATCTCCt                                              >  2:33541/1‑150 (MQ=255)
                                                                                                         gatgGCAGACCACTTAGTTTAACTATTTGCGGTTGAAGGTGCCGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCAACTGGATCGTCAATGATCATGCAGCGCAGACCATAAATGCGCTCAATCAGTTCAGCAGTGACAATCTCCtt                                             >  2:179351/1‑150 (MQ=255)
                                                                                                         gatgGCAGACCACTTAGTTTAACTATTTGCGGTTGAAGGTGCCGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCAACTGGATCGTCAATGATCATGCAGCGCAGACCATAAATGCGCTCAATCAGTTCAGCAGTGACAATCTCCtt                                             >  2:3107259/1‑150 (MQ=255)
                                                                                                         gatgGCAGACCACTTAGTTTAACTATTTGCGGTTGAAGGTGCCGCTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCAACTGGATCGTCAATGATCATGCAGCGCAGACCATAAATGCGCTCAATCAGTTCAGCAGTGACAATCTCCtt                                             >  2:73486/1‑150 (MQ=255)
                                                                                                         gatgGCAGACCACTTAGTTTAACTATTTGCGGTTGAAGGTGCAGTTCGTCCGAGCGGCACCACAAGCGTCGTTCCGGCAAATGGATCGTCAATGATCATGCAGCGCAGACCATAAATGCGCTCAATCAGATCAGCAGAGACAATCTCCtt                                             >  2:1838810/1‑150 (MQ=255)
                                                                                                            ggCAGACCACTTAGTTTAACTATTTGCGGTTGAAGGTGCCGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCAACTGGATCGTCAATGATCATGCAGCGCAGACCATAAATGCGCTCAATCAGTTCAGCAGTGACAATCTCCTTCgg                                          >  1:1453835/1‑150 (MQ=255)
                                                                                                               aGACCACTTAGTTTAACTATTTGCGGTTGAAGGTGCCGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCAACTGGATCGTCAATGATCATGCAGCGCAGACCATAAATGCGCTCAATCAGTTCAGCAGTGACAATCTCCTTCGgcgc                                       >  1:132203/1‑150 (MQ=255)
                                                                                                               aGACCACTTAGTTTAACTATTTGCGGTTGAAGGTGCCGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCAACTGGATCGTCAATGATCATGCAGCGCAGACCATAAATGCGCTCAATCAGTTCAGCAGTGACAATCTCCTTCGgcgc                                       >  1:2027701/1‑150 (MQ=255)
                                                                                                                gACCACTTAGTTTAACTATTTGCGGTTGAAGGTGCCGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCAACTGGATCGTCAATGATCATGCAGCGCAGACCATAAATGCGCTCAATCAGTTCAGCAGTGACAATCTCCTTCGGCGCt                                      >  2:1284158/1‑150 (MQ=255)
                                                                                                                   cACTTAGTTTAACTATTTGCGGTTGAAGGTGCCGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCAACTGGATCGTCAATGATCATGCAGCGCAGACCATAAATGCGCTCAATCAGTTCAGCAGTGACAATCTCCTTCGGCGCTccc                                   >  2:1778250/1‑150 (MQ=255)
                                                                                                                    aCTTAGTTTAACTATTTGCGGTTGAAGGTGCCGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCAACTGGATCGTCAATGATCATGCAGCGCAGACCATAAATGCGCTCAATCAGTTCAGCAGTGACAATCTCCTTCGGCGCTCCCt                                  <  2:1411768/150‑1 (MQ=255)
                                                                                                                          tttAACTATTTGCGGTTGAAGGTGCCGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCAACTGGATCGTCAATGATCATGCAGCGCAGACCATAAATGCGCTCAATCAGTTCAGCAGTGACAATCTCCTTCGGCGCTCCCTGAGcaa                            >  2:1497243/1‑150 (MQ=255)
                                                                                                                           ttAACTATTTGCGGTTGAAGGTGCCGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCAACTGGATCGTCAATGATCATGCAGCGCAGACCATAAATGCGCTCAATCAGTTCAGCAGTGACAATCTCCTTCGGCGCTCCCTGAGcaac                           >  2:1561498/1‑150 (MQ=255)
                                                                                                                                tATTTGCGGTTGAAGGTGCCGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCAACTGGATCGTCAATGATCATGCAGCGCAGACCATAAATGCGCTCAATCAGTTCAGCAGTGACAATCTCCTTCGGCGCTCCCTGAGCAACAAttt                      >  2:846932/1‑150 (MQ=255)
                                                                                                                                 aTTTGCGGTTGAAGGTGCCGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCAACTGGATCGTCAATGATCATGCAGCGCAGACCATAAATGCGCTCAATCAGTTCAGCAGTGACAATCTCCTTCGGCGCTCCCTGAGCAACAAtttt                     >  2:1249432/1‑150 (MQ=255)
                                                                                                                                 aTTTGCGGTTGAAGGTGCCGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCAACTGGATCGTCAATGATCATGCAGCGCAGACCATAAATGCGCTCAATCAGTTCAGCAGTGACAATCTCCTTCGGCGCTCCCTGAGCAACAAtttt                     >  2:2963496/1‑150 (MQ=255)
                                                                                                                                   ttGCGGTTGAAGGTGCCGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCAACTGGATCGTCAATGATCATGCAGCGCAGACCATAAATGCGCTCAATCAGTTCAGCAGTGACAATCTCCTTCGGCGCTCCCTGAGCAACAATTttcc                   <  2:1664883/150‑1 (MQ=255)
                                                                                                                                   ttGCGGTTGAAGGTGCCGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCAACTGGATCGTCAATGATCATGCAGCGCAGACCATAAATGCGCTCAATCAGTTCAGCAGTGACAATCTCCTTCGGCGCTCCCTGAGCAACAATTttcc                   <  2:2367999/150‑1 (MQ=255)
                                                                                                                                   gtGCGGTTGAAGGTGCCGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCAACTGGATCGTCAATGATCATGCAGCGCAGACCATAAATGCGCTCAATCAGTTCAGCAGTGACAATCTCCTTCGGCGCTCCCTGAGCAACAATTttcc                   <  2:1664901/149‑1 (MQ=255)
                                                                                                                                    tGCGGTTGAAGGTGCCGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCAACTGGATCGTCAATGATCATGCAGCGCAGACCATAAATGCGCTCAATCAGTTCAGCAGTGACAATCTCCTTCGGCGCTCCCTGAGCAACAATTttccc                  <  1:2089347/150‑1 (MQ=255)
                                                                                                                                       ggTTGAAGGTGCCGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCAACTGGATCGTCAATGATCATGCAGCGCAGACCATAAATGCGCTCAATCAGTTCAGCAGTGACAATCTCCTTCGGCGCTCCCTGAGCAACAATTttcccttc               >  1:2441533/1‑150 (MQ=255)
                                                                                                                                       ggTTGAAGGTGCCGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCAACTGGATCGTCAATGATCATGCAGCGCAGACCATAAATGCGCTCAATCAGTTCAGCAGTGACAATCTCCTTCGGCGCTCCCTGAGCAACAATTttcccttc               <  2:1214596/150‑1 (MQ=255)
                                                                                                                                       ggTTGAAGGTGCCGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCAACTGGATCGTCAATGATCATGCAGCGCAGACCATAAATGCGCTCAATCAGTTCAGCAGTGACAATCTCCTTCGGCGCTCCCTGAGCAACAATTttcccttc               <  2:2668776/150‑1 (MQ=255)
                                                                                                                                             tggTGCCGCTCGGACGAACGGCACCACAAGCGGCGTTCCGGCCACTGGATCGTCAATGATCAGGCAGCGCCGACCATAAATGCGCTCACTCAGGTCAGCAGTGACAATCTCCTTCGGCGCTCCCTGAGCAACAATTTTCCCTTCCCgcaa         >  2:761666/2‑150 (MQ=255)
                                                                                                                                              ggTGCGGCTCGGCCGCGCGGCACCACAAGCGGCGTTCCGGCAACTGGATCGTCAATGATCATGCAGCGCAGACCATAAATGCGCTCAATCAGTTCAGCAGTGACAATCTCCTTCGGCGCTCCCTGAGCAACAATTTTCCCTTCCCgccga        <  2:1521625/150‑4 (MQ=255)
                                                                                                                                              ggTGCCGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCAACTGGATCGTCAATGATCATGCAGCGCAGACCATAAATGCGCTCAATCAGTTCAGCAGTGACAATCTCCTTCGGCGCTCCCTGAGCAACAATTTTCCCTTCCCgcaat        >  2:3043772/1‑150 (MQ=255)
                                                                                                                                              ggTGCCGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCAACTGGATCGTCAATGATCATGCAGCGCAGACCATAAATGCGCTCAATCAGTTCAGCAGTGACAATCTCCTTCGGCGCTCCCTGAGCAACAATTTTCCCTTCCCgcaat        <  2:229485/150‑1 (MQ=255)
                                                                                                                                               gTGCCGCTCGGACGAACGGCACCACAAGCGGCGTTCCGGCAACTGGATCGTCAATGATCATGCAGCGCAGACCATAAATGCGCTCAATCAGTTCAGCAGTGACAATCTCCTTCGGCGCTCCCTGAGCAACAATTTTCCCTTCCCgcaatg       >  1:3090538/1‑150 (MQ=255)
                                                                                                                                               gTGCCGCTCGGACGAACGGCACCACAAGCGGCGTTCCGGCAACAGGATCGTCAATGATCATGCAGCGCAGACCATAAATGCGCTCAATCAGTTCAGCAGTGACAATCTCCGTCGGCGCTCCCTGAGCAACAATTTTCCCTTCCCgcaatg       >  1:2483102/1‑150 (MQ=255)
                                                                                                                                                tGCCGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCAACTGGATCGTCAATGATCATGCAGCGCAGACCATAAATGCGCTCAATCAGTTCAGCAGTGACAATCTCCTTCGGCGCTCCCTGAGCAACAAATTTCCCTTCCCgcaatgc      >  1:1682615/1‑150 (MQ=255)
                                                                                                                                                 gCCGCTCGGACGAACGGCACCACAAGCGGCGTTCCGGCAACTGGATCGTCAATGATCATGCAGCGCAGACCATAAATGCGCTCAATTAGTTCAGCAGTGACAATCTCCTTCGGCGCTCCCTGAGCAACAATTTTCCCTTCCCgcaatgca     >  2:2604434/1‑150 (MQ=255)
                                                                                                                                                 gCCGCTCGGACGAACGGCACCACAAGCGGCGTTCCGGCAACTGGATCGTCAATGATCATGCAGCGCAGACCATAAATGCGCTCAATCAGTTCAGCAGTGACAATCTCCTTCGGCGCTCCCTGAGCAACAATGTTCCCTTCCCgcaatgca     >  2:2794130/1‑150 (MQ=255)
                                                                                                                                                  ccGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCAACTGGATCGTCAATGATCATGCAGCGCAGACCATAAATGCGCTCAATCAGTTCAGCAGTGACAATCTCCTTCGGCGCTCCCTGAGCAACAATTTTCCCTTCCCgcaatgcaa    <  1:455278/150‑1 (MQ=255)
                                                                                                                                                  ccGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCAACTGGATCGTCAATGATCATGCAGCGCAGACCATAAATGCGCTCAATCAGTTCAGCAGTGACAATCTCCTTCGGCGCTCCCTGAGCAACAATTTTCCCTTCCCgcaatgcaa    <  2:665467/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
GGGTAAGGCGATTATTGTGATCCTTTCCGCGTTGATCGGCGGGATGGTGGCTTGTGGTGGCGTGCTGTTGCGCTATGCGATGGCATCCAGAAAACAGGATGCCATGATGGCAGACCACTTAGTTTAACTATTTGCGGTTGAAGGTGCCGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCAACTGGATCGTCAATGATCATGCAGCGCAGACCATAAATGCGCTCAATCAGTTCAGCAGTGACAATCTCCTTCGGCGCTCCCTGAGCAACAATTTTCCCTTCCCGCAATGCAATC  >  NC_000913/619261‑619558

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: