Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 619,414 T→G 7.7% G262G (GGA→GGC) ‡ fepC ← ferrienterobactin ABC transporter ATPase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913619,4140TG 7.7% 527.6 / 27.6 162G262G (GGA→GGC) ‡fepCferrienterobactin ABC transporter ATPase
Reads supporting (aligned to +/- strand):  ref base T (85/62);  new base G (7/6);  total (92/70)
Fisher's exact test for biased strand distribution p-value = 7.79e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 163 total aligned reads displayed.

GCGATTATTGTGATCCTTTCCGCGTTGATCGGCGGGATGGTGGCTTGTGGTGGCGTGCTGTTGCGCTATGCGATGGCATCCAGAAAACAGGATGCCATGATGGCAGACCACTTAGTTTAACTATTTGCGGTTGAAGGTGCCGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCAACTGGATCGTCAATGATCATGCAGCGCAGACCATAAATGCGCTCAATCAGTTCAGCAGTGACAATCTCCTTCGGCGCTCCCTGAGCAACAATTTTCCCTTCCCGCAATGCAATCAAATG  >  NC_000913/619268‑619563
                                                                                                                                                  |                                                                                                                                                     
gCGATTATTGTGATCCTTTCCGCGTTGATCGGCGGGATGGTGGCTTGTGGTGGCGTGCTGTTGCGCTATGCGATGGCATCCAGAAAACAGGATGCCATGATGGCAGACCACTTAGTTTAACTATTTGCGGTTGAAGGTGCCGTTCGTCCg                                                                                                                                                    >  2:1061954/1‑150 (MQ=255)
gCGATTATTGTGATCCTTTCCGCGTTGATCGGCGGGATGGTGGCTTGTGGTGGCGTGCTGTTGCGCTATGCGATGGCATCCAGAAAACAGGATGCCATGATGGCAGACCACTTAGTTTAACTATTTGCGGTTGAAGGTGCCGTTCGTCCg                                                                                                                                                    >  1:653342/1‑150 (MQ=255)
  gATTTTTGTGATCCTTTCCGCGTTGATGGGCGGGATGGTGGCTTGTTGTGGCGTTCTGTTTGGCTATTCGATGGCATACAGAAATCAGTATTCCATGTTTGCATACCACTTATTTTAACTATTTGCGGTTGAAGGTGCCGTTCGTCGGAg                                                                                                                                                  <  2:656122/150‑1 (MQ=255)
      attGTGATCCGTTCCGCGTTGATCGGCGGGATGGTGGCTTGTGGTGGCGTGCTGTTGCGCTATGCGATGGCATCCAGAAAACAGGATGCCATGATGGCAGACCACTTAGTTTAACTATTTGCGGTTGAAGGTGCCGCTCGGACGAACGGc                                                                                                                                              <  1:1091345/150‑1 (MQ=255)
        tgtgATCCTTTCCGCGTTGATCGGCGGGATGGTGGCTTGTGGTGGCGTGCTGTTGCGCTATGCGATGGCATCCAGAAAACAGGATGCCATGATGGCAGACCACTTAGTTTAACTATTTGCGGTTGAAGGTGCCGCTCGGACGAACGGcac                                                                                                                                            <  2:2446236/150‑1 (MQ=255)
          tgATCCTTTCCGCGTTGATCGGCGGGATGGTGGCTTGTGGTGGCGTGCTGTTGCGCTATGCGATGGCATCCAGAAAACAGGATGCCATGATGGCAGACCACTTAGTTTAACTATTTGCGGTTGAAGGTGCCGTTCGTCCGAGCGGcacca                                                                                                                                          >  2:2602953/1‑150 (MQ=255)
            aTCCTTTCCGCGTTGATCGGCGGGATGGTGGCTTGTGGTGGCGTGCTGTTGCGCTATGCGATGGCATCCAGAAAACAGGATGCCATGATGGCAGACCACTTAGTTTAACTATTTGCGGTTGAAGGTGCCGTTCGTCCGAGCGGCACcaca                                                                                                                                        >  1:1400459/1‑150 (MQ=255)
             tCCTTTCCGCGTTGATCGGCGGGATGGTGGCTTGTGGTGGCGTGCTGTTGCGCTATGCGATGGCATCCAGAAAACAGGATGCCATGATGGCAGACCACTTAGTTTAACTATTTGCGGTTGAAGGTGCCGTTCGTCCGAGCGGCACCACaa                                                                                                                                       >  2:2089347/1‑150 (MQ=255)
              ccTGTCCGCGTTGATCGGCGGGATGGTGGCTTGTGGTGGCGTGCTGTTGCGCTATGCGATGGCATCCAGAAAACAGGATGCCATGATGGCAGACCACTTAGTTTAACTATTTGCGGTTGAAGGTGCCGCTCGGACGAACGGcaccttcaa                                                                                                                                      <  2:1604438/150‑6 (MQ=255)
                tttCCGCGTTGATCGGCGGGATGGTGGCTTGTGGTGGCGTGCTGTTGCGCTATGCGATGGCATCCAGAAAACAGGATGCCATGATGGCAGACCACTTAGTTTAACTATTTGCGGTTGAAGGTGCCGTTCGTCCGAGCGGCACCACAAgcg                                                                                                                                    <  1:1641371/150‑1 (MQ=255)
                     gcgTTGATCGGCGGGATGGTGGCTTGTGGTGGCGTGCTGTTGCGCTATGCGATGGCATCCAGAAAACAGGATGCCATGATGGCAGACCACTTAGTTTAACTATTTGCGGTTGAAGGTGCCGTTCGTCCGAGCGGCACCACAAGCGGCGtt                                                                                                                               >  2:720107/1‑150 (MQ=255)
                     gcgTTCATCCGCGGGATGGTGGCTTGTGGTGGCGTGCTGTTGCGCTATGCGATGGCATCCAGAAAACAGGATGCCATGATGGCAGACCACTTAGTTTAACTATTTGCGGTTGAAGGTGCCGTTCGTCCGAGCGGCACCACAAGCGGCGtt                                                                                                                               >  1:1842048/1‑150 (MQ=255)
                      cgTTGATCGGCGGGATGGTGGCTTGTGGTGGCGTGCTGTTGCGCTATGCGATGGCATCCAGAAAACAGGATGCCATGATGGCAGACCACTTAGTTTAACTATTTGCGGTTGAAGGTGCCGTTCGTCCGAGCGGCACCACAAGCGGCGTTc                                                                                                                              >  1:2252679/1‑150 (MQ=255)
                       gTTGATCGGCGGGATGGTGGCTTGTGGTGGCGTGCTGTTGCGCTATGCGATGGCATCCAGAAAACAGGATGCCATGATGGCAGACCACTTAGTTTAACTATTTGCGGTTGAAGGTGCCGTTCGTCCGAGCGGCACCACAAGCGGCGTTcc                                                                                                                             >  1:1686715/1‑150 (MQ=255)
                       gTTGATCGGCGGGATGGTGGCTTGTGGTGGCGTGCTGTTGCGCTATGCGATGGCATCCAGAAAACAGGATGCCATGATGGCAGACAACTTAGTTTATCTATTTGCGGTTGAAGGTGCCGTTCGTCCGAGCGGCACCACAAGCGGCGTTcc                                                                                                                             <  2:2631868/150‑1 (MQ=255)
                        ttGATCGGCGGGATGGTGGCTTGTGGTGGCGTGCTGTTGCGCTATGCGATGGCATCCAGAAAACAGGATGCCATGATGGCAGACCACTTAGTTTAACTATTTGCGGTTGAAGGTGCCGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCg                                                                                                                            >  2:3080359/1‑150 (MQ=255)
                         tGATCGGCGGGATGGTGGCTTGTGGTGGCGTGCTGTTGCGCTATGCGATGGCATCCAGAAAACAGGATGCCATGATGGCAGACCACTTTGTTTAACTTTTTGCGGTTGAAGGTGCCGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCgg                                                                                                                           <  1:2881184/150‑1 (MQ=255)
                         tGAGCGGCGGGTTGGTGGGTTGTGGTGGCGTGCTGTTGCGCTATGCGATGGCATCCATAAAACAGGATGCCTTGATGGCAGACCACTTAGTTTAACTATTTGCGGTTGAAGGTGCCGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCgg                                                                                                                           <  1:2881246/150‑1 (MQ=255)
                           aTCGGCGGGATGGTGGCTTGTGGTGGCGTGCTGTTGCGCTATGCGATGGCATCCAGAAAACAGGATGCCATGATGGCAGACCACTTAGTTTAACTATTTGCGGTTGAAGGTGCCGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCa                                                                                                                         >  1:954887/1‑150 (MQ=255)
                            tCGGCGGGATGGTGGCTTGTGGTGGCGTGCTGTTGCGCTATGCGATGGCATCCAGAAAACAGTATGCCATGATGGCAGACCACTTAGTTTAACTATTTGCGGTTGAAGGTGACGCTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCac                                                                                                                        >  1:1521625/1‑149 (MQ=255)
                               gcggGATGGTGGCTTGTGGTGGCGTGCTGTTGCGCTATGCGATGGCATCCAGAAAACAGGATGCCATGATGGCAGACCACTTAGTTTAACTATTTGCGGTTGAAGGTGCCGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCAACTg                                                                                                                     >  1:2494298/1‑150 (MQ=255)
                                  ggATGGTGGCTTGTGGTGGCGTGCTGTTGCGCTATGCGATGGCATCCAGAAAACAGGATGCCATGATGGCAGACCACTTAGTTTAACTATTTGCGGTTGAAGGTGCCGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCAACTGGAt                                                                                                                  <  1:2558924/150‑1 (MQ=255)
                                   gctggtggCTTGTGGTGGCGTGCTGTTGCGCTATGCGATGGCATCCAGAAAACAGGATGCCATGATGGCAGACCACTTAGTTTAACTATTTGCGGTTGAAGGTGCCGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCAACTGGATc                                                                                                                 >  1:3062402/3‑150 (MQ=255)
                                   gATGGTGGCTTGTGGTGGCGTGCTGTTGCGCTATGCGATGGCATCCAGAAAACAGGATGCCATGATGGCAGACCACTTAGTTTACCTATTTGCGGTTGAAGGTGCCGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCAACTGGATc                                                                                                                 <  1:1166400/150‑1 (MQ=255)
                                   gATGGTGGCTTGTGGTGGCGTGCTGTTGCGCTATGCGATGGCATCCAGAAAACAGGATGCCATGATGGCAGACCACTTAGTTTAACTATTTGCGGTTGAAGGTGCCGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCAACTGGATc                                                                                                                 <  2:274917/150‑1 (MQ=255)
                                    aTGGTGGCTTGTGGTGGCGTGCTGTTGCGCTATGCGATGGCATCCAGAAAACAGGATGCCATGATGGCAGACCACTTAGTTTAACTATTTGCGGTTGAAGGTGCCGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCAACTGGATCg                                                                                                                >  2:455278/1‑150 (MQ=255)
                                        tggCTTGTGGTGGCGTGCTGTTGCGCTATGCGATGGCATCCAGAAAACAGGATGCCATGATGGCAGACCACTTAGTTTAACTATTTGCGGTTGAAGGTGCCGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCAACTGGATCGTCaa                                                                                                            <  2:472792/150‑1 (MQ=255)
                                             tgtgGTGGCGTGCTGTTGCGCTATGCGATGGCATCCAGAAAACAGGATGCCATGATGGCAGACCACTTAGTTTAACTATTTGCGGTTGAAGGTGCCGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCAACTGGATCGTCAATGATc                                                                                                       <  1:1916867/150‑1 (MQ=255)
                                              gtggtgGCGTGCTGTTGCGCTATGCGATGGCATCCAGAAAACAGGATGCCATGATGGCAGACCACTTAGTTTAACTATTTGCGGTTGAAGGTGCCGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCACCTGGATCGTCAATGATCa                                                                                                      >  1:1235783/1‑150 (MQ=255)
                                               tggtggCGTGCTGTTGCGCTATGCGATGGCATCCAGAAAACAGGATGCCATGATGGCAGACCACTTAGTTTCACGATTTGCGGTTGAAGGTGCCGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCAACTGGATCGTCAATGATCag                                                                                                     >  2:3067377/1‑149 (MQ=255)
                                               tggtggCGTGCTGTTGCGCTATGCGATGGCATCCAGAAAACAGGATGCCATGATGGCAGACCACTTAGTTTAACTATTTGCGGTTGAAGGTGCCGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCAACTGGATCGTCAATGATCAt                                                                                                     >  2:711122/1‑150 (MQ=255)
                                               tggtggCGTGCTGTTGCGCTATGCGATGGCATCCAGAAAACAGGATGCCATGATGGCAGACCACTTAGTTTAACTATTTGCGGTTGAAGGTGCCGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCAACTGGATCGTCAATGATCAt                                                                                                     >  1:2763879/1‑150 (MQ=255)
                                               tggtggCGTGCTGTTGCGCTATGCGATGGCATCCAGAAAACAGGATGCCATGATGGCAGACCACTTAGTTTAACTATTTGCGGTTGAAGGTGCCGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCAACTGGATCGTCAATGATCAt                                                                                                     >  1:1577578/1‑150 (MQ=255)
                                               tggaggCGTGCTGTAGCGCTATGCGATGGCATCCAGAAAACAGGAAGCCATGATGTCAGACCACATAGTTTAACTATTTGCGGTTGAAGGTGCCGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCAACTGGATCGTCAAAGATCaa                                                                                                     >  2:1196241/1‑149 (MQ=255)
                                                  tggCGTGCTGTTGCGCTATGCGATGGCATCCAGAAAACAGGATGCCATGATGGCAGACCACTTAGTTTAACTATTTGCGGTTGAAGGTGCCGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCAACTGGATCGTCAATGATCATGCa                                                                                                  >  1:1232161/1‑150 (MQ=255)
                                                  tggCGTGCTGTTGCGCTATGCGATGGCATCCAGAAAACAGGATGCCATGATGGCAGACCACTTAGTTTAACTATTTGCGGTTGAAGGTGCCGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCAACTGGATCGTCAATGATCATGCa                                                                                                  <  2:356078/150‑1 (MQ=255)
                                                  tggCGTGCTGTTGCGCTATGCGATGGCATCCAGAAAACAGGATGCCATGATGGCAGACCACTTAGTTTAACTATTTGCGGTTGAAGGTGCCGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCAACTGGATCGTCAATGATCATGCa                                                                                                  >  1:233935/1‑150 (MQ=255)
                                                    gCGTGCTGTTGCGCTATGCGATGGCATCCAGAAAACAGGATGCCATGATGGCAGACCACTTAGTTTAACTATTTGCGGTTGAAGGTGCCGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCAACTGGATCGTCAATGATCATGCAgc                                                                                                <  2:2329400/150‑1 (MQ=255)
                                                    gCGTGCTGTTGCGCTATGCGATGGCATCCAGAAAACAGGATGCCATGATGGCAGACCACTTAGTTTAACTATTTGCGGTTGAAGGTGCCGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCAACTGGATCGTCAATGATCATGCAgc                                                                                                <  2:222951/150‑1 (MQ=255)
                                                    gCGTGCTGTTGCGCTATGCGATGGCATCCAGAAAACAGGATGCCATGATGGCAGACCACTTAGTTTAACTATTTGCGGTTGAAGGTGCCGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCAACTGGATCGTCAATGATCATGCAgc                                                                                                <  1:2612710/150‑1 (MQ=255)
                                                       ttcTGTTGCGCTTTGCGATGGCATCCAGATAACTGGATTCTTTGATGGCAGACCACTTAGTTTAACTATTTGCGGTTGATGGTGCCTTTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCAACTGGATCGTCATTGATCATGCAGCGCa                                                                                             <  1:1138485/148‑1 (MQ=255)
                                                              gcgcTATGCGATGGCATCCAGAAAACAGGATGCCATGATGGCAGACCACTTAGTTTAACTATTTGCGGTTGAAGGTGCCGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCAACTGGATCGTCAATGATCATGCAGCGCAGACCATa                                                                                      >  1:2450211/1‑150 (MQ=255)
                                                                  tATGCGATGGCATCCAGAAAACAGGATGCCATGATGGCAGACCACTTAGTTTAACTATTTGCGGTTGAAGGTGCCGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCAACTGGATCGTCAATGATCATGCAGCGCAGACCATAAATg                                                                                  >  2:3118230/1‑150 (MQ=255)
                                                                   aTGCGATGGCATCCAGAAAACAGGATGCCATGATGGCAGACCACTTAGTTTAACTATGTGCGGTTGAAGGTGCCGGTCGTCCGAGCGGCACCACCAGCGGCGTTCCGGCAACTGGATCGTCAATGATCATGCAGCGCAGACCATAAATgc                                                                                 <  1:1647235/150‑1 (MQ=255)
                                                                    tGCGATGGCATCCAGAAAACAGGATGCCATGATGGCAGACCACTTAGTTTAACTATTTGCGGTTGAAGGTGCCGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCAACTGGATCGTCAATGATCATGCAGCGCAGACCATAAATgcg                                                                                >  1:2492388/1‑150 (MQ=255)
                                                                    tGCGATGGCATCCAGAAAACAGGATGCCATGATGGCAGACCACTTAGTTTAACTATTTGCGGTTGAAGGTGCCGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCAACTGGATCGTCAATGATCATGCAGCGCAGACCATAAATgcg                                                                                <  2:85150/150‑1 (MQ=255)
                                                                            cATCCAGAAAACAGGATGCCATGATGGCAGACCACTTAGTTTAACTATTTGCGGTTGAAGGTGCCGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCAACTGGATCGTCAATGATCATGCAGCGCAGACCATAAATGCGCTCAATCa                                                                        <  1:223967/150‑1 (MQ=255)
                                                                              tCCAGAAAACAGGATGCCATGATGGCAGACCACTTAGTTTAACTATTTGCGGTTGAAGGTGCCGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCAACTGGATCGTCAATGATCATGCAGCGCAGACCATAAATGCGCTCAATCAGt                                                                      >  2:3044144/1‑150 (MQ=255)
                                                                                   aaaaCAGGATGCCATGATGGCAGACCACTTAGTTTAACTATTTGCGGTTGAAGGTGCCGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCAACTGGATCGTCAATGATCATGCAGCGCAGACCATAAATGCGCTCAATCAGTTcagc                                                                 >  1:2708114/1‑150 (MQ=255)
                                                                                   aaaaCAGGATGCCATGATGGCAGACCACTTAGTTTAACTATTTGCGGTTGAAGGTGCCGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCAACTGGATCGTAAATTATCATGCAGCGCAGACCATAAAGGCGCTCAATCAGTTaagc                                                                 >  1:1997631/1‑150 (MQ=255)
                                                                                    aaaCTGGATGCCATGATGGCAGACCACTTAGTTTAACTATTTGCGGTAGAAGGTGCCGTTCGTCACAGCGGCACCACAATCGGCGTTCCGGCAACTGGATCGTCAATGATCATGCAGCGCAGACCATAAATGCGCTCAATCAGTTcagca                                                                >  2:883364/1‑150 (MQ=255)
                                                                                    aaaCAGGATGCCATGATGGCAGACCACTTAGTTTAACTATTTGCGGTTGACGGTGCCGTGCGGCCGTGCGGCACCACAAGCGGCGGTCCGGCAACTGGGTCGTCAATGATCATGCAGCGCAGACCCGACATGCGCTCAATCTGTGccgca                                                                >  2:1941165/1‑150 (MQ=255)
                                                                                    aaaCAGGATGCCATGATGGCAGACCACTTAGTTTAACTATTTGCGGTTGAAGGTGCCGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCAACTGGATCGTCAATGATCATGCAGCGCAGACCATAAATGCGCTCAATCAGTTcagca                                                                >  2:1321431/1‑150 (MQ=255)
                                                                                        aGGATGCCATGATGGCAGACCACTTAGTTTAACTATTTGCGGTTGAAGGTGCCGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCAACTGGATCGTCAATGATCATGCAGCGCAGACCATAAATGCGCTCAATCAGTTCAGCAGTGa                                                            >  2:1350677/1‑150 (MQ=255)
                                                                                           aTGCCATGATGGCAGACCACTTAGTTTAACTATTTGCGGTTGAAGGTGCCGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCAACTGGATCGTCAATGATCATGCAGCGCAGACCATAAATGCGCTCAATCAGTTCAGCAGTGACaa                                                         <  1:1024910/150‑1 (MQ=255)
                                                                                           aTGCCATGATGGCAGACCACTTAGTTTAACTATTTGCGGTTGAAGGTGCCGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCAACTGGATCGTCAATGATCATGCAGCGCAGACCATAAATGCGCTCAATCAGTTCAGCAGTGACaa                                                         <  2:272384/150‑1 (MQ=255)
                                                                                                tttttgGCAGACAATTTTTTTTATTTTTTGGGGGTTTATGGTGCCGCTGTCCCGAGCGCCACCACAAGCGGCGTTCCGGCAACTGGATCGTCAATGATCATGCAGCGCAGACCATAAATGCGCTCAATCAGTTCAGCAGTGACAATCTcc                                                    <  1:372241/146‑1 (MQ=255)
                                                                                                atgatgGCAGACCACTTAGTTTAACTATTTGCGGTTGAAGGTGCCGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCAACTGGATCGTCAATGATCATGCAGCGCAGACCATAAATGCGCTCAATCAGTTCAGCAGTGACAATCTcc                                                    >  2:2773638/1‑150 (MQ=255)
                                                                                                atgatgGCAGACCACTTAGTTTAACTATTTGCGGTTGAAGGTGCCGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCAACTGGATCGTCAATGATCATGCAGCGCAGACCATAAATGCGCTCAATCAGTTCAGCAGTGACAATCTcc                                                    <  2:577814/150‑1 (MQ=255)
                                                                                                atgatgGCAGACCACTTAGTTTAACTATTTGCGGTTGAAGGTGCCGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCAACTGGATCGTCAATGATCATGCAGCGCAGACCATAAATGCGCTCAATCAGTTCAGCAGTGACAATCTcc                                                    >  2:2497315/1‑150 (MQ=255)
                                                                                                atgatgGCAGACCACTTAGTTTAACTATTTGCGGTTGAAGGTGCCGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCAACTGGATCGTCAATGATCATGCAGCGCAGACCATAAATGCGCTCAATCAGTTCAGCAGTGACAATCTcc                                                    >  2:2497263/1‑150 (MQ=255)
                                                                                                  gatgGCAGACCACTTAGTTTAACTATTTGCGGTTGAAGGTGCCGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCAACTGGATCGTCAATGATCATGCAGCGCAGACCATAAATGCGCTCAATCAGTTCAGCAGTGACAATCTCCtt                                                  >  2:179351/1‑150 (MQ=255)
                                                                                                  gatgGCAGACCACTTAGTTTAACTATTTGCGGTTGAAGGTGCCGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCAACTGGATCGTCAATGATCATGCAGCGCAGACCATAAATGCGCTCAATCAGTTCAGCAGTGACAATCTCCtt                                                  >  2:3107259/1‑150 (MQ=255)
                                                                                                  gatgGCAGACCACTTAGTTTAACTATTTGCGGTTGAAGGTGCCGCTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCAACTGGATCGTCAATGATCATGCAGCGCAGACCATAAATGCGCTCAATCAGTTCAGCAGTGACAATCTCCtt                                                  >  2:73486/1‑150 (MQ=255)
                                                                                                  gatgGCAGACCACTTAGTTTAACTATTTGCGGTTGAAGGTGCAGTTCGTCCGAGCGGCACCACAAGCGTCGTTCCGGCAAATGGATCGTCAATGATCATGCAGCGCAGACCATAAATGCGCTCAATCAGATCAGCAGAGACAATCTCCtt                                                  >  2:1838810/1‑150 (MQ=255)
                                                                                                     ggCAGACCACTTAGTTTAACTATTTGCGGTTGAAGGTGCCGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCAACTGGATCGTCAATGATCATGCAGCGCAGACCATAAATGCGCTCAATCAGTTCAGCAGTGACAATCTCCTTCgg                                               >  1:1453835/1‑150 (MQ=255)
                                                                                                        aGACCACTTAGTTTAACTATTTGCGGTTGAAGGTGCCGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCAACTGGATCGTCAATGATCATGCAGCGCAGACCATAAATGCGCTCAATCAGTTCAGCAGTGACAATCTCCTTCGgcgc                                            >  1:132203/1‑150 (MQ=255)
                                                                                                         gACCACTTAGTTTAACTATTTGCGGTTGAAGGTGCCGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCAACTGGATCGTCAATGATCATGCAGCGCAGACCATAAATGCGCTCAATCAGTTCAGCAGTGACAATCTCCTTCGGCGCt                                           >  2:1284158/1‑150 (MQ=255)
                                                                                                          aCCACTTAGTTTAACTATTTGCGGTTGAAGGTGCCGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCAACTGGATCGTCAATGATCATGCAGCGCAGACCATAAATGCGCTCAATCAGTTCAGCAGTGACAATCTCCTTCGGCGCTc                                          >  1:1299261/1‑150 (MQ=255)
                                                                                                           ccACTTAGTTTAACTATTTGCGGTTGAAGGTGCCGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCAACTGGATCGTCAATGATCATGCAGCGCAGACCATAAATGCGCTCAATCAGTTCAGCAGTGACAATCTCCTTCGGCGCTcc                                         >  2:2755687/1‑150 (MQ=255)
                                                                                                            cACTTAGTTTAACTATTTGCGGTTGAAGGTGCCGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCAACTGGATCGTCAATGATCATGCAGCGCAGACCATAAATGCGCTCAATCAGTTCAGCAGTGACAATCTCCTTCGGCGCTccc                                        >  2:1778250/1‑150 (MQ=255)
                                                                                                             aCTTAGTTTAACTATTTGCGGTTGAAGGTGCCGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCAACTGGATCGTCAATGATCATGCAGCGCAGACCATAAATGCGCTCAATCAGTTCAGCAGTGACAATCTCCTTCGGCGCTCCCt                                       <  2:1411768/150‑1 (MQ=255)
                                                                                                                  gTTTAACTATTTGCGGTTGAAGGTGCCGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCAACTGGATCGTCAATGATCATGCAGCGCAGACCATAAATGCGCTCAATCAGTTCAGCAGTGACAATCTCCTTCGGCGCTCCCTGAGca                                  <  1:808149/150‑1 (MQ=255)
                                                                                                                   tttAACTATTTGCGGTTGAAGGTGCCGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCAACTGGATCGTCAATGATCATGCAGCGCAGACCATAAATGCGCTCAATCAGTTCAGCAGTGACAATCTCCTTCGGCGCTCCCTGAGcaa                                 >  2:1497243/1‑150 (MQ=255)
                                                                                                                    ttAACTATTTGCGGTTGAAGGTGCCGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCAACTGGATCGTCAATGATCATGCAGCGCAGACCATAAATGCGCTCAATCAGTTCAGCAGTGACAATCTCCTTCGGCGCTCCCTGAGcaac                                >  2:1561498/1‑150 (MQ=255)
                                                                                                                         tATTTGCGGTTGAAGGTGCCGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCAACTGGATCGTCAATGATCATGCAGCGCAGACCATAAATGCGCTCAATCAGTTCAGCAGTGACAATCTCCTTCGGCGCTCCCTGAGCAACAAttt                           >  2:846932/1‑150 (MQ=255)
                                                                                                                          aTTTGCGGTTGAAGGTGCCGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCAACTGGATCGTCAATGATCATGCAGCGCAGACCATAAATGCGCTCAATCAGTTCAGCAGTGACAATCTCCTTCGGCGCTCCCTGAGCAACAAtttt                          >  2:2963496/1‑150 (MQ=255)
                                                                                                                          aTTTGCGGTTGAAGGTGCCGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCAACTGGATCGTCAATGATCATGCAGCGCAGACCATAAATGCGCTCAATCAGTTCAGCAGTGACAATCTCCTTCGGCGCTCCCTGAGCAACAAtttt                          >  2:1249432/1‑150 (MQ=255)
                                                                                                                            ttGCGGTTGAAGGTGCCGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCAACTGGATCGTCAATGATCATGCAGCGCAGACCATAAATGCGCTCAATCAGTTCAGCAGTGACAATCTCCTTCGGCGCTCCCTGAGCAACAATTttcc                        <  2:1664883/150‑1 (MQ=255)
                                                                                                                            ttGCGGTTGAAGGTGCCGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCAACTGGATCGTCAATGATCATGCAGCGCAGACCATAAATGCGCTCAATCAGTTCAGCAGTGACAATCTCCTTCGGCGCTCCCTGAGCAACAATTttcc                        <  2:2368242/150‑1 (MQ=255)
                                                                                                                            ttGCGGTTGAAGGTGCCGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCAACTGGATCGTCAATGATCATGCAGCGCAGACCATAAATGCGCTCAATCAGTTCAGCAGTGACAATCTCCTTCGGCGCTCCCTGAGCAACAATTttcc                        <  2:2367999/150‑1 (MQ=255)
                                                                                                                            gtGCGGTTGAAGGTGCCGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCAACTGGATCGTCAATGATCATGCAGCGCAGACCATAAATGCGCTCAATCAGTTCAGCAGTGACAATCTCCTTCGGCGCTCCCTGAGCAACAATTttcc                        <  2:1664901/149‑1 (MQ=255)
                                                                                                                             tGCGGTTGAAGGTGCCGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCAACTGGATCGTCAATGATCATGCAGCGCAGACCATAAATGCGCTCAATCAGTTCAGCAGTGACAATCTCCTTCGGCGCTCCCTGAGCAACAATTttccc                       <  1:2089347/150‑1 (MQ=255)
                                                                                                                                ggTTGAAGGTGCCGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCAACTGGATCGTCAATGATCATGCAGCGCAGACCATAAATGCGCTCAATCAGTTCAGCAGTGACAATCTCCTTCGGCGCTCCCTGAGCAACAATTttcccttc                    <  2:3015613/150‑1 (MQ=255)
                                                                                                                                ggTTGAAGGTGCCGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCAACTGGATCGTCAATGATCATGCAGCGCAGACCATAAATGCGCTCAATCAGTTCAGCAGTGACAATCTCCTTCGGCGCTCCCTGAGCAACAATTttcccttc                    <  2:1214596/150‑1 (MQ=255)
                                                                                                                                ggTTGAAGGTGCCGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCAACTGGATCGTCAATGATCATGCAGCGCAGACCATAAATGCGCTCAATCAGTTCAGCAGTGACAATCTCCTTCGGCGCTCCCTGAGCAACAATTttcccttc                    >  1:2441533/1‑150 (MQ=255)
                                                                                                                                      tggTGCCGCTCGGACGAACGGCACCACAAGCGGCGTTCCGGCCACTGGATCGTCAATGATCAGGCAGCGCCGACCATAAATGCGCTCACTCAGGTCAGCAGTGACAATCTCCTTCGGCGCTCCCTGAGCAACAATTTTCCCTTCCCgcaa              >  2:761666/2‑150 (MQ=255)
                                                                                                                                       ggTGCGGCTCGGCCGCGCGGCACCACAAGCGGCGTTCCGGCAACTGGATCGTCAATGATCATGCAGCGCAGACCATAAATGCGCTCAATCAGTTCAGCAGTGACAATCTCCTTCGGCGCTCCCTGAGCAACAATTTTCCCTTCCCgccga             <  2:1521625/150‑4 (MQ=255)
                                                                                                                                       ggTGCCGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCAACTGGATCGTCAATGATCATGCAGCGCAGACCATAAATGCGCTCAATCAGTTCAGCAGTGACAATCTCCTTCGGCGCTCCCTGAGCAACAATTTTCCCTTCCCgcaat             <  2:229485/150‑1 (MQ=255)
                                                                                                                                        gTGCCGCTCGGACGAACGGCACCACAAGCGGCGTTCCGGCAACAGGATCGTCAATGATCATGCAGCGCAGACCATAAATGCGCTCAATCAGTTCAGCAGTGACAATCTCCGTCGGCGCTCCCTGAGCAACAATTTTCCCTTCCCgcaatg            >  1:2483102/1‑150 (MQ=255)
                                                                                                                                         tGCCGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCAACTGGATCGTCAATGATCATGCAGCGCAGACCATAAATGCGCTCAATCAGTTCAGCAGTGACAATCTCCTTCGGCGCTCCCTGAGCAACAATTTTCCCTTCCCgcaatgc           >  2:2800299/1‑150 (MQ=255)
                                                                                                                                          gCCGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCAACTGGATCGTCAATGATCATGCAGCGCAGACCATAAATGCGCTCAATCAGTTCAGCAGTGACAATCTCCTTCGGCGCTCCCTGAGCAACAATTTTCCCTTCCCgcaatgca          <  2:514636/150‑1 (MQ=255)
                                                                                                                                          gCCGCTCGGACGAACGGCACCACAAGCGGCGTTCCGGCCCCTGGATCGTCAATGATCATGCAGCGCAGACCAGCAATGCGCTCAATCAGTTCAGCAGTGACAAGCTCCGGCGGCGCTCCCTGAGCACCAATTTTCCCTTCCCgcacggca          >  2:2794035/1‑145 (MQ=255)
                                                                                                                                           ccGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCAACTGGATCGTCAATGATCATGCAGCGCAGACCATAAATGCGCTCAATCAGTTCAGCAGTGACAATCTCCTTCGGCGCTCCCTGAGCAACAATTTTCCCTTCCCgcaatgcaa         <  1:2003158/150‑1 (MQ=255)
                                                                                                                                           ccGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCAACTGGATCGTCAATGATCATGCAGCGCAGACCATAAATGCGCTCAATCAGTTCAGCAGTGACAATCTCCTTCGGCGCTCCCTGAGCAACAATTTTCCCTTCCCgcaatgcaa         <  2:665467/150‑1 (MQ=255)
                                                                                                                                           ccGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCAACTGGATCGTCAATGATCATGCAGCGCAGACCATAAATGCGCTCAATCAGTTCAGCAGTGACAATCTCCTTCGGCGCTCCCTGAGCAACAATTTTCCCTTCCCgcaatgcaa         <  1:2764538/150‑1 (MQ=255)
                                                                                                                                           ccGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCAACTGGATCGTCAATGATCATGCAGCGCAGACCATAAATGCGCTCAATCAGTTCAGCAGTGACAATCTCCTTCGGCGCTCCCTGAGCAACAATTTTCCCTTCCCgcaatgcaa         <  1:455278/150‑1 (MQ=255)
                                                                                                                                             gTTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCAACTGGATCGTCAATGATCATGCAGCGCAGACCATAAATGCGCTCAATCAGTTCAGCAGTGACAATCTCCTTCGGCGCTCCCTGAGCAACAATTTTCCCTTCCCGCAATGCAATc       <  2:2663524/150‑1 (MQ=255)
                                                                                                                                                 gTCCGAGCGGCACCACAAGCGGCGTTCCGGCAACTGGATCGTCAATGATCATGCAGCGCAGACCATAAATGCGCTCAATCAGTTCAGCAGTGACAATCTCCTTCGGCGCTCCCTGAGCAACAATTTTCCCTTCCCGCAATGCAATCAAAt   >  2:588779/1‑150 (MQ=255)
                                                                                                                                                  tCCGAGCGGCACCACAAGCGGCGTTCCGGCAACTGGATCGTCAATGATCATGCAGCGCAGACCATAAATGCGCTCAATCAGTTCAGCAGTGACAATCTCCTTCGGCGCTCCCTGAGCAACAATTTTCCCTTCCCGCAATGCAATCAAAtg  <  1:1159032/150‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                     
GCGATTATTGTGATCCTTTCCGCGTTGATCGGCGGGATGGTGGCTTGTGGTGGCGTGCTGTTGCGCTATGCGATGGCATCCAGAAAACAGGATGCCATGATGGCAGACCACTTAGTTTAACTATTTGCGGTTGAAGGTGCCGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCAACTGGATCGTCAATGATCATGCAGCGCAGACCATAAATGCGCTCAATCAGTTCAGCAGTGACAATCTCCTTCGGCGCTCCCTGAGCAACAATTTTCCCTTCCCGCAATGCAATCAAATG  >  NC_000913/619268‑619563

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: