Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 619,419 G→A 6.7% L261F (CTC→TTC)  fepC ← ferrienterobactin ABC transporter ATPase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913619,4190GA 6.7% 544.0 / 17.1 164L261F (CTC→TTC) fepCferrienterobactin ABC transporter ATPase
Reads supporting (aligned to +/- strand):  ref base G (84/69);  new base A (6/5);  total (90/74)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 165 total aligned reads displayed.

GATTATTGTGATCCTTTCCGCGTTGATCGGCGGGATGGTGGCTTGTGGTGGCGTGCTGTTGCGCTATGCGATGGCATCCAGAAAACAGGATGCCATGATGGCAGACCACTTAGTTTAACTATTTGCGGTTGAAGGTGCCGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCAACTGGATCGTCAATGATCATGCAGCGCAGACCATAAATGCGCTCAATCAGTTCAGCAGTGACAATCTCCTTCGGCGCTCCCTGAGCAACAATTTTCCCTTCCCGCAATGCAATCAAATGGCTG  >  NC_000913/619270‑619567
                                                                                                                                                     |                                                                                                                                                    
gATTTTTGTGATCCTTTCCGCGTTGATGGGCGGGATGGTGGCTTGTTGTGGCGTTCTGTTTGGCTATTCGATGGCATACAGAAATCAGTATTCCATGTTTGCATACCACTTATTTTAACTATTTGCGGTTGAAGGTGCCGTTCGTCGGAg                                                                                                                                                      <  2:656122/150‑1 (MQ=255)
   tattGTGATCCTTTCCGCGTTGATCGGCGGGATGGTGGCTTGTGGTGGCGTGCTGTTGCGCTATGCGATGGCATCCAGAAAACAGGATGCCATGATGGCAGACCACTTAGTTTAACTATTTGCGGTTGAAGGTGCCGTTCGTCCGAGCgg                                                                                                                                                   <  1:1573125/150‑1 (MQ=255)
    attGTGATCCGTTCCGCGTTGATCGGCGGGATGGTGGCTTGTGGTGGCGTGCTGTTGCGCTATGCGATGGCATCCAGAAAACAGGATGCCATGATGGCAGACCACTTAGTTTAACTATTTGCGGTTGAAGGTGCCGCTCGGACGAACGGc                                                                                                                                                  <  1:1091345/150‑1 (MQ=255)
      tgtgATCCTTTCCGCGTTGATCGGCGGGATGGTGGCTTGTGGTGGCGTGCTGTTGCGCTATGCGATGGCATCCAGAAAACAGGATGCCATGATGGCAGACCACTTAGTTTAACTATTTGCGGTTGAAGGTGCCGCTCGGACGAACGGcac                                                                                                                                                <  2:2446067/150‑1 (MQ=255)
        tgATCCTTTCCGCGTTGATCGGCGGGATGGTGGCTTGTGGTGGCGTGCTGTTGCGCTATGCGATGGCATCCAGAAAACAGGATGCCATGATGGCAGACCACTTAGTTTAACTATTTGCGGTTGAAGGTGCCGTTCGTCCGAGCGGcacca                                                                                                                                              >  1:665467/1‑150 (MQ=255)
        tgATCCTTTCCGCGTTGATCGGCGGGATGGTGGCTTGTGGTGGCGTGCTGTTGCGCTATGCGATGGCATCCAGAAAACAGGATGCCATGATGGCAGACCACTTAGTTTAACTATTTGCGGTTGAAGGTGCCGTTCGTCCGAGCGGcacca                                                                                                                                              >  2:2602953/1‑150 (MQ=255)
          aTCCTTTCCGCGTTGATCGGCGGGATGGTGGCTTGTGGTGGCGTGCTGTTGCGCTATGCGATGGCATCCAGAAAACAGGATGCCATGATGGCAGACCACTTAGTTTAACTATTTGCGGTTGAAGGTGCCGTTCGTCCGAGCGGCACcaca                                                                                                                                            >  1:1400459/1‑150 (MQ=255)
            ccTGTCCGCGTTGATCGGCGGGATGGTGGCTTGTGGTGGCGTGCTGTTGCGCTATGCGATGGCATCCAGAAAACAGGATGCCATGATGGCAGACCACTTAGTTTAACTATTTGCGGTTGAAGGTGCCGCTCGGACGAACGGcaccttcaa                                                                                                                                          <  2:1604438/150‑6 (MQ=255)
              tttCCGCGTTGATCGGCGGGATGGTGGCTTGTGGTGGCGTGCTGTTGCGCTATGCGATGGCATCCAGAAAACAGGATGCCATGATGGCAGACCACTTAGTTTAACTATTTGCGGTTGAAGGTGCCGTTCGTCCGAGCGGCACCACAAgcg                                                                                                                                        <  1:1641371/150‑1 (MQ=255)
                   gcgTTGATCGGCGGGATGGTGGCTTGTGGTGGCGTGCTGTTGCGCTATGCGATGGCATCCAGAAAACAGGATGCCATGATGGCAGACCACTTATTTTAACTATTTGCGGTTGAAGGTGCCGTTCGTCCGAGAGGCACCACAAGCGGCGtt                                                                                                                                   >  2:2122718/1‑150 (MQ=255)
                   gcgTTGATCGGCGGGATGGTGGCTTGTGGTGGCGTGCTGTTGCGCTATGCGATGGCATCCAGAAAACAGGATGCCATGATGGCAGACCACTTAGTTTAACTATTTGCGGTTGAAGGTGCCGTTCGTCCGAGCGGCACCACAAGCGGCGtt                                                                                                                                   >  2:720107/1‑150 (MQ=255)
                   gcgTTCATCCGCGGGATGGTGGCTTGTGGTGGCGTGCTGTTGCGCTATGCGATGGCATCCAGAAAACAGGATGCCATGATGGCAGACCACTTAGTTTAACTATTTGCGGTTGAAGGTGCCGTTCGTCCGAGCGGCACCACAAGCGGCGtt                                                                                                                                   >  1:1842048/1‑150 (MQ=255)
                    cgTTGATCGGCGGGATGGTGGCTTGTGGTGGCGTGCTGTTGCGCTATGCGATGGCATCCAGAAAACAGGATGCCATGATGGCAGACCACTTAGTTTAACTATTTGCGGTTGAAGGTGCCGTTCGTCCGAGCGGCACCACAAGCGGCGTTc                                                                                                                                  >  1:2252679/1‑150 (MQ=255)
                     gTTGATCGGCGGGATGGTGGCTTGTGGTGGCGTGCTGTTGCGCTATGCGATGGCATCCAGAAAACAGGATGCCATGATGGCAGACCACTTAGTTTAACTATTTGCGGTTGAAGGTGCCGTTCGTCCGAGCGGCACCACAAGCGGCGTTcc                                                                                                                                 >  1:1686715/1‑150 (MQ=255)
                      ttGATCGGCGGGATGGTGGCTTGTGGTGGCGTGCTGTTGCGCTATGCGATGGCATCCAGAAAACAGGATGCCATGATGGCAGACCACTTAGTTTAACTATTTGCGGTTGAAGGTGCCGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCg                                                                                                                                <  2:1771482/150‑1 (MQ=255)
                      ttGATCGGCGGGATGGTGGCTTGTGGTGGCGTGCTGTTGCGCTATGCGATGGCATCCAGAAAACAGGATGCCATGATGGCAGACCACTGAGTTTAACTATTTGCGGTTGAAGGTGCCGTTCGTCCGAGCGGCACCACCAGCGGCGTTCCg                                                                                                                                >  2:3080371/1‑150 (MQ=255)
                       tGATCGGCGGGATGGTGGCTTGTGGTGGCGTGCTGTTGCGCTATGCGATGGCATCCAGAAAACAGGATGCCATGATGGCAGACCACTTTGTTTAACTTTTTGCGGTTGAAGGTGCCGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCgg                                                                                                                               <  1:2881184/150‑1 (MQ=255)
                       tGATCGGCGGGATGGTGGCTTGTGGTGGCGTGCTGTTGCGCTATGCGAGGGAATCCAGAAAACAGGAGGCCATGTTGGCAGACCACTTTGTTTAACTTTTTGCGGTTGAAGGTGCCGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCgg                                                                                                                               <  1:2881234/150‑1 (MQ=255)
                         aTCGGCGGGATGGTGGCTTGTGGTGGCGTGCTGTTGCGCTATGCGATGGCATCCAGAAAACAGGATGCCATGATGGCAGACCACTTAGTTTAACTATTTGCGGTTGAAGGTGCCGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCa                                                                                                                             >  1:954887/1‑150 (MQ=255)
                           cggcggGATGGTGGCTTGTGGTGGCGTGCTGTTGCGCTATGCGATGGCATCCAGAAAACAGGATGCCATGATGGCAGACCACTTAGTTTAACTATTTGCGGTTGAAGGTGCCGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCAAc                                                                                                                           <  1:2223809/150‑1 (MQ=255)
                                ggATGGTGGCTTGTGGTGGCGTGCTGTTGCGCTATGCGATGGCATCCAGAAAACAGGATGCCATGATGGCAGACCACTTAGTTTAACTATTTGCGGTTGAAGGTGCCGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCAACTGGAt                                                                                                                      <  1:2558924/150‑1 (MQ=255)
                                 gctggtggCTTGTGGTGGCGTGCTGTTGCGCTATGCGATGGCATCCAGAAAACAGGATGCCATGATGGCAGACCACTTAGTTTAACTATTTGCGGTTGAAGGTGCCGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCAACTGGATc                                                                                                                     >  1:3062402/3‑150 (MQ=255)
                                 gATGGTGGCTTGTGGTGGCGTGCTGTTGCGCTATGCGATGGCATCCAGAAAACAGGATGCCATGATGGCAGACCACTTAGTTTACCTATTTGCGGTTGAAGGTGCCGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCAACTGGATc                                                                                                                     <  1:1166400/150‑1 (MQ=255)
                                 gATGGTGGCTTGTGGTGGCGTGCTGTTGCGCTATGCGATGGCATCCAGAAAACAGGATGCCATGATGGCAGACCACTTAGTTTAACTATTTGCGGTTGAAGGTGCCGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCAACTGGATc                                                                                                                     <  2:274917/150‑1 (MQ=255)
                                  aTGGTGGCTTGTGGTGGCGTGCTGTTGCGCTATGCGATGGCATCCAGAAAACAGGATGCCATGATGGCAGACCACTTAGTTTAACTATTTGCGGTTGAAGGTGCCGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCAACTGGATCg                                                                                                                    >  2:455278/1‑150 (MQ=255)
                                            gtggtgGCGTGCTGTTGCGCTATGCGATGGCATCCAGAAAACAGGATGCCATGATGGCAGACCACTTAGTTTAACTATTTGCGGTTGAAGGTGCCGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCACCTGGATCGTCAATGATCa                                                                                                          >  1:1235783/1‑150 (MQ=255)
                                             tggtggCGTGCTGTTGCGCTATGCGATGGCATCCAGAAAACAGGATGCCATGATGGCAGACCACTTAGTTTCACGATTTGCGGTTGAAGGTGCCGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCAACTGGATCGTCAATGATCag                                                                                                         >  2:3067377/1‑149 (MQ=255)
                                             tggtggCGTGCTGTTGCGCTATGCGATGGCATCCAGAAAACAGGATGCCATGATGGCAGACCACTTAGTTTAACTATTTGCGGTTGAAGGTGCCGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCAACTGGATCGTCAATGATCAt                                                                                                         >  2:711122/1‑150 (MQ=255)
                                             tggtggCGTGCTGTTGCGCTATGCGATGGCATCCAGAAAACAGGATGCCATGATGGCAGACCACTTAGTTTAACTATTTGCGGTTGAAGGTGCCGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCAACTGGATCGTCAATGATCAt                                                                                                         >  1:1577578/1‑150 (MQ=255)
                                             tggtggCGTGCTGTTGCGCTATGCGATGGCATCCAGAAAACAGGATGCCATGATGGCAGACCACTTAGTTTAACTATTTGCGGTTGAAGGTGCCGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCAACTGGATCGTCAATGATCAt                                                                                                         >  1:2763879/1‑150 (MQ=255)
                                             tggtggCGTGCTGTTGCGCTATGCGATGGCATCCAGAAAACAGGATGCCATGATGGCAGACCACTTAGTTTAACTATTTGCGGTTGAAGGTGCCGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCAACTGGATCGACAATGATCAt                                                                                                         >  2:3067358/1‑150 (MQ=255)
                                             tggaggCGTGCTGTAGCGCTATGCGATGGCATCCAGAAAACAGGAAGCCATGATGTCAGACCACATAGTTTAACTATTTGCGGTTGAAGGTGCCGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCAACTGGATCGTCAAAGATCaa                                                                                                         >  2:1196241/1‑149 (MQ=255)
                                                tggCGTGCTGTTGCGCTATGCGATGGCATCCAGAAAACAGGATGCCATGATGGCAGACCACTTAGTTTAACTATTTGCGGTTGAAGGTGCCGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCAACTGGATCGTCAATGATCATGCa                                                                                                      >  1:2548038/1‑150 (MQ=255)
                                                tggCGTGCTGTTGCGCTATGCGATGGCATCCAGAAAACAGGATGCCATGATGGCAGACCACTTAGTTTAACTATTTGCGGTTGAAGGTGCCGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCAACTGGATCGTCAATGATCATGCa                                                                                                      >  1:233935/1‑150 (MQ=255)
                                                tggCGTGCTGTTGCGCTATGCGATGGCATCCAGAAAACAGGATGCCATGATGGCAGACCACTTAGTTTAACTATTTGCGGTTGAAGGTGCCGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCAACTGGATCGTCAATGATCATGCa                                                                                                      <  2:356079/150‑1 (MQ=255)
                                                tggCGTGCTGTTGCGCTATGCGATGGCATCCAGAAAACAGGATGCCATGATGGCAGACCACTTAGTTTAACTATTTGCGGTTGAAGGTGCCGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCAACTGGATCGTCAATGATCATGCa                                                                                                      >  1:1232161/1‑150 (MQ=255)
                                                     ttcTGTTGCGCTTTGCGATGGCATCCAGATAACTGGATTCTTTGATGGCAGACCACTTAGTTTAACTATTTGCGGTTGATGGTGCCTTTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCAACTGGATCGTCATTGATCATGCAGCGCa                                                                                                 <  1:1138485/148‑1 (MQ=255)
                                                     tGCTGTTGCGCTATGCGATGGCATCCAGAAAACAGGATGCCATGATGGCAGACCACTTAGTTTAACTATTTGCGGTTGAAGGTGCCGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCAACTGGATCGTCAATGATCATGCAGCGCa                                                                                                 <  2:2354298/150‑1 (MQ=255)
                                                            gcgcTATGCGATGGCATCCAGAAAACAGGATGCCATGATGGCAGACCACTTAGTTTAACTATTTGCGGTTGAAGGTGCCGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCAACTGGATCGTCCATGATCATGCAGCGCAGACCATa                                                                                          >  1:2450220/1‑150 (MQ=255)
                                                               cTATGCGATGGCATCCAGAAAACAGGATGCCATGATGGCAGACCACTTAGTTTAACTATTTGCGGTTGAAGGTGCCGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCAACTGGATCGTCAATGATCATGCAGCGCAGACCATAAAt                                                                                       >  2:1055914/1‑150 (MQ=255)
                                                                  tGCGATGGCATCCAGAAAACAGGATGCCATGATGGCAGACCACTTAGTTTAACTATTTGCGGTTGAAGGTGCCGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCAACTGGATCGTCAATGATCATGCAGCGCAGACCATAAATgcg                                                                                    >  1:2492388/1‑150 (MQ=255)
                                                                  tGCGATGGCATCCAGAAAACAGGATGCCATGATGGCAGACCACTTAGTTTAACTATTTGCGGTTGAAGGTGCCGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCAACTGGATCGTCAATGATCATGCAGCGCAGACCATAAATgcg                                                                                    <  2:85150/150‑1 (MQ=255)
                                                                  tGCGATGGCATCCAGAAAACAGGATGCCATGATGGCAGACCACTTAGTTTAACTATTTGCGGTTGAAGGTGCCGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCAACTGGATCGTCAATGATCATGCAGCGCAGACCAGAAATgcg                                                                                    >  2:523278/1‑150 (MQ=255)
                                                                     gATGGCATCCAGAAAACAGGATGCCATGATGGCAGACCACTTAGTTTAACTATTTGCGGTTGAAGGTGCCGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCAACTGGATCGTCAATGATCATGCAGCGCAGACCATAAATGCGCTc                                                                                 <  1:1967565/150‑1 (MQ=255)
                                                                            tCCAGAAAACAGGATGCCATGATGGCAGACCACTTAGTTTAACTATTTGCGGTTGAAGGTGCCGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCAACTGGATCGTCAATGATCATGCAGCGCAGACCATAAATGCGCTCAATCAGt                                                                          >  2:3044144/1‑150 (MQ=255)
                                                                                 aaaaCAGGATGCCATGATGGCAGACCACTTAGTTTAACTATTTGCGGTTGAAGGTGCCGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCAACTGGATCGTAAATTATCATGCAGCGCAGACCATAAAGGCGCTCAATCAGTTaagc                                                                     >  1:1997631/1‑150 (MQ=255)
                                                                                  aaaCTGGATGCCATGATGGCAGACCACTTAGTTTAACTATTTGCGGTAGAAGGTGCCGTTCGTCACAGCGGCACCACAATCGGCGTTCCGGCAACTGGATCGTCAATGATCATGCAGCGCAGACCATAAATGCGCTCAATCAGTTcagca                                                                    >  2:883364/1‑150 (MQ=255)
                                                                                  aaaCAGGATGCCATGATGGCAGACCACTTAGTTTAACTATTTGCGGTTGACGGTGCCGTGCGGCCGTGCGGCACCACAAGCGGCGGTCCGGCAACTGGGTCGTCAATGATCATGCAGCGCAGACCCGACATGCGCTCAATCTGTGccgca                                                                    >  2:1941165/1‑150 (MQ=255)
                                                                                  aaaCAGGATGCCATGATGGCAGACCACTTAGTTTAACTATTTGCGGTTGAAGGTGCCGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCAACTGGATCGTCAATGATCATGCAGCGCAGACCATAAATGCGCTCAATCAGTTcagca                                                                    >  2:1321431/1‑150 (MQ=255)
                                                                                   aaCAGGATGCCATGATGGCAGACCACTTAGTTTAACTATTTGCGGTTGAAGGTGCCGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCAACTGGATCGTCAATGATCATGCAGCGCAGACCATAAATGCGCTCAATCAGTTcagcag                                                                   <  1:2654/150‑1 (MQ=255)
                                                                                     cAGGATGCCATGATGGCAGACCACTTAGTTTAACTATTTGCGGTTGAAGGTGCCGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCAACTGGATCGTCAATGATCATGCAGCGCAGACCATAAATGCGCTCAATCAGTTCAGCAGTg                                                                 <  2:489219/150‑1 (MQ=255)
                                                                                      aGGATGCCATGATGGCAGACCACTTAGTTTAACTATTTGCGGTTGAAGGTGCCGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCAACTGGATCGTCAATGATCATGCAGCGCAGACCATAAATGCGCTCAATCAGTTCAGCAGTGa                                                                >  2:1350677/1‑150 (MQ=255)
                                                                                         aTGCCATGATGGCAGACCACTTAGTTTAACTATTTGCGGTTGAAGGTGCCGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCAACTGGATCGTCAATGATCATGCAGCGCAGACCATAAATGCGCTCAATCAGTTCAGCAGTGACaa                                                             <  1:1024910/150‑1 (MQ=255)
                                                                                         aTGCCATGATGGCAGACCACTTAGTTTAACTATTTGCGGTTGAAGGTGCCGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCAACTGGATCGTCAATGATCATGCAGCGCAGACCATAAATGCGCTCAATCAGTTCAGCAGTGACaa                                                             <  2:272384/150‑1 (MQ=255)
                                                                                              tttttgGCAGACAATTTTTTTTATTTTTTGGGGGTTTATGGTGCCGCTGTCCCGAGCGCCACCACAAGCGGCGTTCCGGCAACTGGATCGTCAATGATCATGCAGCGCAGACCATAAATGCGCTCAATCAGTTCAGCAGTGACAATCTcc                                                        <  1:372241/146‑1 (MQ=255)
                                                                                              atgatgGCAGACCACTTAGTTTAACTATTTGCGGTTGAAGGTGCCGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCAACTGGATCGTCAATGATCATGCAGCGCAGACCATAAATGCGCTCAATCAGTTCAGCAGTGACAATCTcc                                                        >  2:2497263/1‑150 (MQ=255)
                                                                                              atgatgGCAGACCACTTAGTTTAACTATTTGCGGTTGAAGGTGCCGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCAACTGGATCGTCAATGATCATGCAGCGCAGACCATAAATGCGCTCAATCAGTTCAGCAGTGACAATCTcc                                                        <  2:2494298/150‑1 (MQ=255)
                                                                                              atgatgGCAGACCACTTAGTTTAACTATTTGCGGTTGAAGGTGCCGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCAACTGGATCGTCAATGATCATGCAGCGCAGACCATAAATGCGCTCAATCAGTTCAGCAGTGACAATCTcc                                                        >  2:2773638/1‑150 (MQ=255)
                                                                                               tgatgGCAGACCACTTAGTTTAACTATTTGCGGTTGAAGGTGCCGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCAACTGGATCGTCAATGATCATGCAGCGCAGACCATAAATGCGCTCAATCAGTTCAGCAGTGACAATCTCCt                                                       >  2:33541/1‑150 (MQ=255)
                                                                                                gatgGCAGACCACTTAGTTTAACTATTTGCGGTTGAAGGTGCCGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCAACTGGATCGTCAATGATCATGCAGCGCAGACCATAAATGCGCTCAATCAGTTCAGCAGTGACAATCTCCtt                                                      >  2:1838828/1‑150 (MQ=255)
                                                                                                gatgGCAGACCACTTAGTTTAACTATTTGCGGTTGAAGGTGCCGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCAACTGGATCGTCAATGATCATGCAGCGCAGACCATAAATGCGCTCAATCAGTTCAGCAGTGACAATCTCCtt                                                      >  2:3107259/1‑150 (MQ=255)
                                                                                                gatgGCAGACCACTTAGTTTAACTATTTGCGGTTGAAGGTGCCGCTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCAACTGGATCGTCAATGATCATGCAGCGCAGACCATAAATGCGCTCAATCAGTTCAGCAGTGACAATCTCCtt                                                      >  2:73486/1‑150 (MQ=255)
                                                                                                   ggCAGACCACTTAGTTTAACTATTTGCGGTTGAAGGTGCCGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCAACTGGATCGTCAATGATCATGCAGCGCAGACCATAAATGCGCTCAATCAGTTCAGCAGTGACAATCTCCTTCgg                                                   >  1:1453835/1‑150 (MQ=255)
                                                                                                      aGACCACTTAGTTTAACTATTTGCGGTTGAAGGTGCCGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCAACTGGATCGTCAATGATCATGCAGCGCAGACCATAAATGCGCTCAATCAGTTCAGCAGTGACAATCTCCTTCGgcgc                                                >  1:2027701/1‑150 (MQ=255)
                                                                                                      aGACCACTTAGTTTAACTATTTGCGGTTGAAGGTGCCGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCAACTGGATCGTCAATGATCATGCAGCGCAGACCATAAATGCGCTCAATCAGTTCAGCAGTGACAATCTCCTTCGgcgc                                                >  1:132203/1‑150 (MQ=255)
                                                                                                        aCCACTTAGTTTAACTATTTGCGGTTGAAGGTGCCGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCAACTGGATCGTCAATGATCATGCAGCGCAGACCATAAATGCGCTCAATCAGTTCAGCAGTGACAATCTCCTTCGGCGCTc                                              >  1:1299261/1‑150 (MQ=255)
                                                                                                         ccACTTAGTTTAACTATTTGCGGTTGAAGGTGCCGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCAACTGGATCGTCAATGATCATGCAGCGCAGACCATAAATGCGCTCAATCAGTTCAGCAGTGACAATCTCCTTCGGCGCTcc                                             >  2:2755687/1‑150 (MQ=255)
                                                                                                          cACTTAGTTTAACTATTTGCGGTTGAAGGTGCCGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCAACTGGATCGTCAATGATCATGCAGCGCAGACCATAAATGCGCTCAATCAGTTCAGCAGTGACAATCTCCTTCGGCGCTccc                                            >  2:1778250/1‑150 (MQ=255)
                                                                                                                gTTTAACTATTTGCGGTTGAAGGTGCCGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCAACTGGATCGTCAATGATCATGCAGCGCAGACCATAAATGCGCTCAATCAGTTCAGCAGTGACAATCTCCTTCGGCGCTCCCTGAGca                                      >  2:1567919/1‑150 (MQ=255)
                                                                                                                 tttAACTATTTGCGGTTGAAGGTGCCGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCAACTGGATCGTCAATGATCATGCAGCGCAGACCATAAATGCGCTCAATCAGTTCAGCAGTGACAATCTCCTTCGGCGCTCCCTGAGcaa                                     >  2:1497243/1‑150 (MQ=255)
                                                                                                                      cTATTTGCGATTGAAGGTGCCGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCAACTGGATCGTCAATGATCATGCAGCGCAGACCATAAATGCGCTCAATCAGTTCAGCAGTGACAATCTCCTTCGGCGCTCCCTGAGCAACAAtt                                >  2:1291829/1‑150 (MQ=255)
                                                                                                                       tATTTGCGGTTGAAGGTGCCGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCAACTGGATCGTCAATGATCATGCAGCGCAGACCATAAATGCGCTCAATCAGTTCAGCAGTGACAATCTCCTTCGGCGCTCCCTGAGCAACAAttt                               >  2:846932/1‑150 (MQ=255)
                                                                                                                        aTTTGCGGTTGAAGGTGCCGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCAACTGGATCGTCAATGATCATGCAGCGCAGACCATAAATGCGCTCAATCAGTTCAGCAGTGACAATCTCCTTCGGCGCTCCCTGAGCAACAAtttt                              >  2:1249432/1‑150 (MQ=255)
                                                                                                                        aTTTGCGGTTGAAGGTGCCGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCAACTGGATCGTCAATGATCATGCAGCGCAGACCATAAATGCGCTCAATCAGTTCAGCAGTGACAATCTCCTTCGGCGCTCCCTGAGCAACAAtttt                              >  2:2963496/1‑150 (MQ=255)
                                                                                                                          ttGCGGTTGAAGGTGCCGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCAACTGGATCGTCAATGATCATGCAGCGCAGACCATAAATGCGCTCAATCAGTTCAGCAGTGACAATCTCCTTCGGCGCTCCCTGAGCAACAATTttcc                            <  2:2367999/150‑1 (MQ=255)
                                                                                                                          ttGCGGTTGAAGGTGCCGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCAACTGGATCGTCAATGATCATGCAGCGCAGACCATAAATGCGCTCAATCAGTTCAGCAGTGACAATCTCCTTCGGCGCTCCCTGAGCAACAATTttcc                            <  2:1664883/150‑1 (MQ=255)
                                                                                                                              ggTTGAAGGTGCCGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCAACTGGATCGTCAATGATCATGCAGCGCAGACCATAAATGCGCTCAATCAGTTCAGCAGTGACAATCTCCTTCGGCGCTCCCTGAGCAACAATTttcccttc                        <  2:1475187/150‑1 (MQ=255)
                                                                                                                              ggTTGAAGGTGCCGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCAACTGGATCGTCAATGATCATGCAGCGCAGACCATAAATGCGCTCAATCAGTTCAGCAGTGACAATCTCCTTCGGCGCTCCCTGAGCAACAATTttcccttc                        >  1:2441533/1‑150 (MQ=255)
                                                                                                                              ggTTGAAGGTGCCGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCAACTGGATCGTCAATGATCATGCAGCGCAGACCATAAATGCGCTCAATCAGTTCAGCAGTGACAATCTCCTTCGGCGCTCCCTGAGCAACAATTttcccttc                        >  1:2719957/1‑150 (MQ=255)
                                                                                                                              ggTTGAAGGTGCCGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCAACTGGATCGTCAATGATCATGCAGCGCAGACCATAAATGCGCTCAATCAGTTCAGCAGTGACAATCTCCTTCGGCGCTCCCTGAGCAACAATTttcccttc                        <  2:3015613/150‑1 (MQ=255)
                                                                                                                              ggTTGAAGGTGCCGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCAACTGGATCGTCAATGATCATGCAGCGCAGACCATAAATGCGCTCAATCAGTTCAGCAGTGACAATCTCCTTCGGCGCTCCCTGAGCAACAATTttcccttc                        <  2:1214596/150‑1 (MQ=255)
                                                                                                                                 tGAAGGTGCCGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCAACTGGATCGTCAATGATCATGCAGCGCAGACCATAAATGCGCTCAATCAGTTCAGCAGTGACAATCTCCTTCGGCGCTCCCTGAGCAACAATTTTCCCTTCCCg                     <  2:2288145/150‑1 (MQ=255)
                                                                                                                                    tggTGCCGCTCGGACGAACGGCACCACAAGCGGCGTTCCGGCCACTGGATCGTCAATGATCAGGCAGCGCCGACCATAAATGCGCTCACTCAGGTCAGCAGTGACAATCTCCTTCGGCGCTCCCTGAGCAACAATTTTCCCTTCCCgcaa                  >  2:761666/2‑150 (MQ=255)
                                                                                                                                     ggTGCGGCTCGGCCGCGCGGCACCACAAGCGGCGTTCCGGCAACTGGATCGTCAATGATCATGCAGCGCAGACCATAAATGCGCTCAATCAGTTCAGCAGTGACAATCTCCTTCGGCGCTCCCTGAGCAACAATTTTCCCTTCCCgccga                 <  2:1521625/150‑4 (MQ=255)
                                                                                                                                     ggTGCCGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCAACTGGATCGTCAATGATCATGCAGCGCAGACCATAAATGCGCTCAATCAGTTCAGCAGTGACAATCTCCTTCGGCGCTCCCTGAGCAACAATTTTCCCTTCCCgcaat                 <  2:229485/150‑1 (MQ=255)
                                                                                                                                      gTGCCGCTCGGACGAACGGCACCACAAGCGGCGTTCCGGCAACAGGATCGTCAATGATCATGCAGCGCAGACCATAAATGCGCTCAATCAGTTCAGCAGTGACAATCTCCGTCGGCGCTCCCTGAGCAACAATTTTCCCTTCCCgcaatg                >  1:2483102/1‑150 (MQ=255)
                                                                                                                                       tGCCGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCAACTGGATCGTCAATGATCATGCAGCGCAGACCATAAATGCGCTCAATCAGTTCAGCAGTGACAATCTCCTTCGGCGCTCCCTGAGCAACAATTTTCCCTTCCCgcaatgc               >  2:2800299/1‑150 (MQ=255)
                                                                                                                                       tGCCGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCAACTGGATCGTCAATGATCATGCAGCGCAGACCATAAATGCGCTCAATCAGTTCAGCAGTGACAATCTCCTTCGGCGCTCCCTGAGCAACAAATTTCCCTTCCCgcaatgc               >  1:1682615/1‑150 (MQ=255)
                                                                                                                                        gCCGCTCGGACGAACGGCACCACAAGCGGCGTTCCGGCCCCTGGATCGTCAATGATCATGCAGCGCAGACCAGCAATGCGCTCAATCAGTTCAGCAGTGACAAGCTCCGGCGGCGCTCCCTGAGCACCAATTTTCCCTTCCCgcacggca              >  2:2794035/1‑145 (MQ=255)
                                                                                                                                        gCCGCTCGGACGAACGGCACCACAAGCGGCGTTCCGGCAACTGGATCGTCAATGATCATGCAGCGCAGACCATAAATGCGCTCAATTAGTTCAGCAGTGACAATCTCCTTCGGCGCTCCCTGAGCAACAATTTTCCCTTCCCgcaatgca              >  2:2604434/1‑150 (MQ=255)
                                                                                                                                         ccGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCAACTGGATCGTCAATGATCATGCAGCGCAGACCATAAATGCGCTCAATCAGTTCAGCAGTGACAATCTCCTTCGGCGCTCCCTGAGCAACAATTTTCCCTTCCCgcaatgcaa             <  2:665467/150‑1 (MQ=255)
                                                                                                                                         ccGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCAACTGGATCGTCAATGATCATGCAGCGCAGACCATAAATGCGCTCAATCAGTTCAGCAGTGACAATCTCCTTCGGCGCTCCCTGAGCAACAATTTTCCCTTCCCgcaatgcaa             >  2:1799110/1‑150 (MQ=255)
                                                                                                                                         ccGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCAACTGGATCGTCAATGATCATGCAGCGCAGACCATAAATGCGCTCAATCAGTTCAGCAGTGACAATCTCCTTCGGCGCTCCCTGAGCAACAATTTTCCCTTCCCgcaatgcaa             <  1:455278/150‑1 (MQ=255)
                                                                                                                                           gTTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCAACTGGATCGTCAATGATCATGCAGCGCAGACCATAAATGCGCTCAATCAGTTCAGCAGTGACAATCTCCTTCGGCGCTCCCTGAGCAACAATTTTCCCTTCCCGCAATGCAATc           <  2:2663524/150‑1 (MQ=255)
                                                                                                                                               gTCCGAGCGGCACCACAAGCGGCGTTCCGGCAACTGGATCGTCAATGATCATGCAGCGCAGACCATAAATGCGCTCAATCAGTTCAGCAGTGACAATCTCCTTCGGCGCTCCCTGAGCAACAATTTTCCCTTCCCGCAATGCAATCAAAt       >  2:588779/1‑150 (MQ=255)
                                                                                                                                                tCCGAGCGGCACCACAAGCGGCGTTCCGGCAACTGGATCGTCAATGATCATGCAGCGCAGACCATAAATGCGCTCAATCAGTTCAGCAGTGACAATCTCCTTCGGCGCTCCCTGAGCAACAATTTTCCCTTCCCGCAATGCAATCAAAtg      <  1:1159032/150‑1 (MQ=255)
                                                                                                                                                 ccGAGCGGCACCACAAGCGGCGTTCCGGCAACTGGATCGTCAATGATCATGCAGCGCAGACCATAAATGCGCTCAATCAGTTCAGCAGTGACAATCTCCTTCGGCGCTCCCTGAGCAACAATTTTCCCTTCCCGCAATGCAATCAAAtgg     <  1:222913/150‑1 (MQ=255)
                                                                                                                                                 ccGAGCGGCACCACAAGCGGCGTTCCGGCAACTGGATCGTCAATGATCATGCAGCGCAGACCATAAATGCGCTCAATCAGTACAGCAGTGACAATCTCCTTCGGCGCTCCCTGAGCAACAATTTTCCCTTCCCGCAATGCAATCAAAtgg     <  2:573884/150‑1 (MQ=255)
                                                                                                                                                   gAGCGGCACCACAAGCGGCGTTCCGGCAACTGGATCGTCAATGATCATGCAGCGCAGACCATAAATGCGCTCAATCAGTTCAGCAGTGACAATCTCCTTCGGCGCTCCCTGAGCAACAATTTTCCCTTCCCGCAATGCAATCAAAtggct   <  2:2548038/150‑1 (MQ=255)
                                                                                                                                                    aGCGGCACCACAAGCGGCGTTCCGGCAACTGGATCGTCAATGATCATGCAGCGCAGACCATAAATGCGCTCAATCAGTTCAGCAGTGACAATCTCCTTCGGCGCTCCCTGAGCAACAATTTTCCCTTCCCGCAATGCAATCAAAtggctg  >  1:2796441/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
GATTATTGTGATCCTTTCCGCGTTGATCGGCGGGATGGTGGCTTGTGGTGGCGTGCTGTTGCGCTATGCGATGGCATCCAGAAAACAGGATGCCATGATGGCAGACCACTTAGTTTAACTATTTGCGGTTGAAGGTGCCGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCAACTGGATCGTCAATGATCATGCAGCGCAGACCATAAATGCGCTCAATCAGTTCAGCAGTGACAATCTCCTTCGGCGCTCCCTGAGCAACAATTTTCCCTTCCCGCAATGCAATCAAATGGCTG  >  NC_000913/619270‑619567

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: