Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,286,944 T→G 6.2% intergenic (+418/+122) narI → / ← rttR nitrate reductase 1, gamma (cytochrome b(NR)) subunit/rtT sRNA, processed from tyrT transcript

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,286,9440TG 6.2% 307.8 / 6.6 117intergenic (+418/+122)narI/rttRnitrate reductase 1, gamma (cytochrome b(NR)) subunit/rtT sRNA, processed from tyrT transcript
Reads supporting (aligned to +/- strand):  ref base T (39/63);  new base G (4/5);  total (48/69)
Fisher's exact test for biased strand distribution p-value = 7.33e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 120 total aligned reads displayed.

GTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCG  >  NC_000913/1286795‑1287092
                                                                                                                                                     |                                                                                                                                                    
gTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCGGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGACt                                                                                                                                                      >  1:2981451/1‑150 (MQ=255)
ctCGAAACCTGGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATAATTGTCTGGGTATTAGTTTGAGTATTGGGGGGCTTTTGCAAGGGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCt                                                                                                                                                      <  2:1181300/149‑1 (MQ=14)
    aaCCTTGTTCGTAGCTTCTCATCGTTACCCGCTTGGGCAGATTTTTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCgg                                                                                                                                                  <  2:421883/150‑1 (MQ=14)
        ttGGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAAc                                                                                                                                              <  2:2630235/150‑1 (MQ=255)
        ttGGTCGAAGCGTCTCATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAAc                                                                                                                                              <  2:2630126/150‑1 (MQ=255)
        ttGGTCGAAGCGTCTCATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCGCAGGCAAc                                                                                                                                              <  2:1595661/150‑1 (MQ=255)
          ggTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCGCAGGCAACga                                                                                                                                            <  2:299044/150‑2 (MQ=255)
          ggTCGAAGATTCTCATCCTTCCCCGCTTGGGCATAATATTTGATTGCGGATTCGTTTGAGAATTCGGGGGCTTTTGAATGTGTTGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCGCAGGCAACga                                                                                                                                            <  2:1319864/150‑2 (MQ=14)
             cGAAGCTTCTGATCCTTACCCGCTTGGGCAGATTATTTGATTGCGGATTAGTTTGAGATTTCGGGTGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCATTGCCTGCGGCAACGctct                                                                                                                                         <  2:62564/150‑1 (MQ=11)
             cGAAGCTTCTCATCCTTCCCCGCTTGGGCAGACTTAATTATTTCGTATTAGTTTGAGTATTCCGGGTCTTTTGTAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCATTGCCTGCGGCAACGctct                                                                                                                                         <  2:62696/150‑1 (MQ=14)
             cGAAGCGTCTCATCCTTCCCCGCTTGGGCAGAATATATGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCATTGCCTGCGGCAACGctct                                                                                                                                         <  2:62690/150‑1 (MQ=255)
                  cTTCTCATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGc                                                                                                                                    >  2:387423/1‑150 (MQ=255)
                    tctcATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGAGCTGCGCGCTTCGCCGTTCGGGTCGTTGCCTGCGGCACCGCTATCTCGCTg                                                                                                                                  >  2:2738383/1‑150 (MQ=255)
                    tctcATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCAGGGGCAAATGAAATTCATCATTGACGGGTAAGCATTACTCATCGCTGCGCGCTTCGCCCATCGGGTCGTTGCCTGCGGGGACGCTGGCTCGCTg                                                                                                                                  >  1:507391/1‑150 (MQ=11)
                    tatcATCCTTTGCCGCTTGGGGAGTATATTCGTTTGCGTAGTAGTTTGAGTTTTCCTTTATTTTTGTAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTg                                                                                                                                  <  2:2879699/148‑1 (MQ=11)
                     ctcATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAGTTCAGGGGCTTTTGAAAGTGATGGTGGTGGGGTAAGGGATACACATCTCTACTCGATTCGCCCTTAGGGAAGTTGCATGCGGCAAAGCTCTCTAGCTGa                                                                                                                                 >  1:1993060/1‑150 (MQ=11)
                     ctcATCCTTCCCCGCTTGGGCAGAATATTTGAGTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGa                                                                                                                                 <  2:2390763/150‑1 (MQ=255)
                        aTCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGgcgc                                                                                                                              >  2:2777347/1‑150 (MQ=255)
                         tcgttCCCCGCTTGGTCAGAATATTTGATTGCGGATTCGTGTGTGTTTTCCGGGGCTTTTGCTAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCt                                                                                                                             <  2:2977097/147‑1 (MQ=14)
                         ccgttCCCCGCTCGGGCAGAATATTTGATTGCGGACTCGTGTGAGAAGTGCGGGTCTTATGCAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCt                                                                                                                             <  2:739049/147‑1 (MQ=14)
                            ttCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGa                                                                                                                          <  1:2777347/150‑1 (MQ=255)
                            ttCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGa                                                                                                                          >  2:488080/1‑150 (MQ=255)
                            ttCCCCGCTTGGGCAGAATAGTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGa                                                                                                                          >  2:1035674/1‑150 (MQ=255)
                               cccGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCCACGCACTCACGCAGACCCACGAGcc                                                                                                                       >  1:924569/1‑150 (MQ=14)
                                ccGCTTTGGCATATTATTTGATTGCGGATTCGTTTGAGAATTCCGGGTCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCg                                                                                                                      <  2:323838/150‑1 (MQ=255)
                                ccGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCAGCGGCAACGCACTCACGCAGACGCTCGAGccc                                                                                                                      >  1:2953414/1‑149 (MQ=255)
                                    ttGGCCAGAATATTTGATTGCGGATTCGATTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAAcc                                                                                                                  <  1:2725770/150‑1 (MQ=255)
                                       ggCAGAATTTTTGATTGCGGATTCGTTTGAGAATTACGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTa                                                                                                               <  2:1807764/150‑1 (MQ=255)
                                       ggCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTa                                                                                                               <  1:1143847/150‑1 (MQ=255)
                                       ggCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCGGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTa                                                                                                               <  1:387423/150‑1 (MQ=255)
                                         cAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGt                                                                                                             >  2:286188/1‑150 (MQ=255)
                                         cAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGt                                                                                                             >  1:203453/1‑150 (MQ=255)
                                         cAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGt                                                                                                             >  2:74595/1‑150 (MQ=255)
                                         cAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCATAGt                                                                                                             >  1:1280814/1‑150 (MQ=255)
                                         cAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCATAGt                                                                                                             >  1:2827083/1‑150 (MQ=255)
                                             atatTTGTTTGTGGATGCGTTTGAGTATTCGTGTTATTTTGTAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGaa                                                                                                         <  1:1920383/150‑1 (MQ=14)
                                             atatTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGAGCGCTTCGCCCTTCGGGTCGTTTCCTCCGGCAACGCTCTCTAGCTGACCCTCGAGCCGAACCGTTGTCGaa                                                                                                         >  2:983023/1‑150 (MQ=14)
                                              tatTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAg                                                                                                        <  1:1353383/150‑1 (MQ=255)
                                              tatTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCAGCGGCAACGCTCTCTCGCTGACGCTCGAGACGAACCTTAGTCGAAg                                                                                                        >  2:616580/1‑150 (MQ=255)
                                                  ttaTTTCGGATTCGTTTGAGTATTCGTGTTCTTTTGTAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTtc                                                                                                    <  2:690654/148‑1 (MQ=14)
                                                    aTTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCAGCGGCACCCCTCTCACGCAGACGCACGAGCCGCACCCTAGTCGAAGCAtctc                                                                                                  >  1:1812026/1‑150 (MQ=14)
                                                     ttGCTGATTCGTTTGAGAATTCCGGTTCTTTTGTAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCa                                                                                                 <  2:2529109/150‑1 (MQ=255)
                                                     ttGCGGATTGGTTTGAGTATTCGGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCa                                                                                                 <  1:2791746/150‑1 (MQ=255)
                                                     ttGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCa                                                                                                 >  2:353352/1‑150 (MQ=255)
                                                      tGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGTTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCAt                                                                                                <  1:1183791/150‑1 (MQ=255)
                                                         ggATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCGCGAGACGAACCATAGTAGAAGCTTCTCaaact                                                                                             >  2:1129414/1‑146 (MQ=14)
                                                           aTTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAAAGCTCTCACGCTGCCGCTCGAGCCGAACCATAGTAGAAGCTTCTcatccatc                                                                                           >  2:611037/1‑150 (MQ=14)
                                                                tttGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCTCTGCGCGCTTCGCCCTTCGGGTCGTTGCCAGCCGCAACGCTCACTCGCTGGCGCTCGAGCCGAACCATGGTCGAAGCTTCTCAGCCTGCCCCGc                                                                                      >  1:856462/1‑150 (MQ=11)
                                                                tttGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCCCTTCGCCCTTCGGGTCGTTTCCTGAGGCAAAGATCTCTCGCTGACGCACGATCCGAACCTTATTCGAAGCTTCTAATACATCCCCGc                                                                                      >  2:684830/1‑150 (MQ=14)
                                                                  tGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCAt                                                                                    <  2:966282/150‑1 (MQ=255)
                                                                      aaTTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCCGCAACGCACACACCCTGACGCACCACCCGAACCTTAGTCGAACCTTCTCAACCGTACCCCCAAGGGc                                                                                >  2:2876899/1‑150 (MQ=14)
                                                                               gCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTTGTCGTAGCTTCTCATCCTTCCCCGCATGGGCAGAATAttt                                                                       <  1:2395314/150‑1 (MQ=25)
                                                                                 ttttGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGa                                                                     <  1:660983/150‑1 (MQ=38)
                                                                                  tttGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGATGCCAGCGGCAACCCTCTCTCCCTGACGCTCGAGCCGAACCATAGTCGAAGCAACTCAACCAACCCCCCAACGGCAGAAAATTTGaa                                                                    >  2:2269287/1‑149 (MQ=11)
                                                                                  tttGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTCCGCCCATCGCCCAACGGCTCGATGCCTTCCGCAACGCACGCACCCAGACGCCCCAACCGAACCTTACTCGAAGCTACACATCAAACCCCCCATGGGCACACTAATTGAt                                                                    >  2:2269309/1‑150 (MQ=2)
                                                                                      gaaGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCg                                                                <  1:2521080/149‑1 (MQ=39)
                                                                                      aaaGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCg                                                                >  2:921930/1‑150 (MQ=39)
                                                                                       aaGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCgg                                                               >  2:1991381/1‑150 (MQ=21)
                                                                                        aGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGa                                                              <  2:1280814/150‑1 (MQ=39)
                                                                                        aGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGa                                                              <  2:2827083/150‑1 (MQ=39)
                                                                                        aGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGa                                                              <  2:203453/150‑1 (MQ=39)
                                                                                                      gggAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGtt                                                <  1:74595/150‑1 (MQ=255)
                                                                                                      gggAAGGAGTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCGTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCttccttaaa                                                <  2:39241/150‑8 (MQ=255)
                                                                                                        gAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCa                                              <  1:2895801/150‑1 (MQ=255)
                                                                                                         caggaggaCTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGGTGCCTGCGGCAACGCTCTCGCGCTGCCGCTCGAGCCGAACCTTAGTCGAAGCTTCTCCTCCTTCCCCGCATGGGCAGAATATTGGATTGCGGATTCACTTGAGAGTTCAg                                             <  2:1804679/143‑1 (MQ=14)
                                                                                                         aaGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAg                                             <  2:1408480/150‑1 (MQ=255)
                                                                                                         aaGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCACTTGAGAGTTCAg                                             <  2:2445377/150‑1 (MQ=255)
                                                                                                          aGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAgg                                            <  1:921930/150‑1 (MQ=255)
                                                                                                          aGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAgg                                            <  1:2934863/150‑1 (MQ=255)
                                                                                                             aTTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGCCGAACCGTAGTCGAAGCTTCTCAACCGTCCCCGCAGGGGCAGAATATTTGATTGCGGATTCGGTGGGGAGTTCAGGGAc                                         >  2:2430836/1‑150 (MQ=11)
                                                                                                                 cTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACtttt                                     <  1:204855/150‑1 (MQ=255)
                                                                                                                     gcgcTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGTCGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGaaa                                 <  1:1755570/150‑1 (MQ=255)
                                                                                                                      cgcTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAg                                <  1:381777/150‑1 (MQ=255)
                                                                                                                      cgcTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAg                                <  1:2447624/150‑1 (MQ=255)
                                                                                                                       gcTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGCCGAACCTTCGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGt                               <  2:1093348/150‑1 (MQ=255)
                                                                                                                       gcTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGCCGCTCGAGCCGAACCTTTGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGTATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGt                               <  2:2804471/150‑1 (MQ=255)
                                                                                                                       gcTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGt                               <  1:1844997/150‑1 (MQ=255)
                                                                                                                       gcTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGt                               <  2:2683693/150‑1 (MQ=255)
                                                                                                                       gcTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGt                               <  1:2428161/150‑1 (MQ=255)
                                                                                                                       gcTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGt                               <  1:1310088/150‑1 (MQ=255)
                                                                                                                         tGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCCAGGCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGA                             <  2:434012‑M1/150‑1 (MQ=255)
                                                                                                                         tGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGA                             <  1:473984‑M1/150‑1 (MQ=255)
                                                                                                                           cgcgcTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGGTTGGGAGTTCAGGGACGTTTGAAAGTGATG                           >  2:954068‑M1/1‑150 (MQ=255)
                                                                                                                           cgcgcTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATG                           <  2:924569‑M1/150‑1 (MQ=255)
                                                                                                                                   gCCCGTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGG                   <  2:2882484‑M1/150‑1 (MQ=255)
                                                                                                                                    cccTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCGTAGTCGAAGCTTCTCATCCTTCCCCGCAGGGGCAGAATAGTTGATTGCGGATTCGCTTGTGCGGTCAGGGACGTTTGAAAGTGAGGGGGGGGGGG                  >  2:786186‑M1/1‑150 (MQ=255)
                                                                                                                                    cccTTCGGGTCGTTGCCTGCGGCAACGCTCTCACCCTGACGCTCGAGCCCAACCTTAGTCGAAGCTTCTCATCCATCCCCGCATGGGCAGCAAATTTTATTTCGGATTCGCGTGGGAGTTCAGGGACTGTTGAAAGTGAGGGTGGTGGGG                  >  2:1177202‑M1/1‑150 (MQ=255)
                                                                                                                                    cccTTCGGGCCGTTGCCCGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTCGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGG                  <  1:3059601‑M1/150‑1 (MQ=255)
                                                                                                                                      cTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGA                <  1:1695082‑M1/150‑1 (MQ=255)
                                                                                                                                      cTTCGGGTCGTTGCCGGCGGCAACGCTCTCTCGCTGACGCACGAGCCGAACCGTAGTCGAAGCGTCTCATCCGTACCCGCAAGGGCCGAATATTTCATTGCGGATTCGGTTGGGAGTTCAGGGACTTTTGAAAGTGATGGGGGGGGGGGa                >  2:2935941/1‑150 (MQ=14)
                                                                                                                                        tCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAG              <  1:1408839‑M1/150‑1 (MQ=255)
                                                                                                                                        tCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAG              <  1:353352‑M1/150‑1 (MQ=255)
                                                                                                                                             tCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTa         <  2:1946636/150‑1 (MQ=255)
                                                                                                                                               gTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACt       <  1:2738383/150‑1 (MQ=255)
                                                                                                                                                ttGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGATTGTACAGGGACGGTGGAGAGTGGTGGTGGTGGGGGAAGGATTACTc      >  2:366704/1‑150 (MQ=17)
                                                                                                                                                ttGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTc      >  2:3099799/1‑150 (MQ=255)
                                                                                                                                                ttGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTc      >  2:1335094/1‑150 (MQ=255)
                                                                                                                                                ttGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTACTGGGAGTGTAGAGAGTGATCGTGGTGGGGGAAGGATTACTc      >  2:3115643/1‑150 (MQ=17)
                                                                                                                                                 tGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGACGAACCTTAGTCGAAGCTTCTCATCCGTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCa     <  1:1602239/150‑1 (MQ=255)
                                                                                                                                                    cTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCATAGTCGAAGCTTCTCAACCATCCCCCCATGGGCCGAAAATTTGATTTCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTaaacacag  >  1:2262688/1‑143 (MQ=17)
                                                                                                                                                     |                                                                                                                                                    
GTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCG  >  NC_000913/1286795‑1287092

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: