Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,368,180 A→G 5.3% T118T (ACA→ACG yhcG → DUF1016 family protein in the PD‑(D/E)XK nuclease superfamily

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,368,1800AG 5.3% 423.0 / 10.2 132T118T (ACA→ACGyhcGDUF1016 family protein in the PD‑(D/E)XK nuclease superfamily
Reads supporting (aligned to +/- strand):  ref base A (78/45);  new base G (4/3);  total (84/48)
Fisher's exact test for biased strand distribution p-value = 7.09e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.29e-01

Only 100 of 137 total aligned reads displayed.

CGACTATCAAAGGATTTATGTCTAAGGTATAAGCGTGGGTTCTCTGCAAAAAACTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGAAAAAGAGACGCTCCGCTGTGGCTG  >  NC_000913/3368031‑3368329
                                                                                                                                                     |                                                                                                                                                     
cGACTATCAAAGGATTTATGTCTAAGGTATAAGCGTGGGTTCTCTGCAAAAAACTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGaca                                                                                                                                                       >  1:2979429/1‑150 (MQ=255)
  gcTATCAAAGGATTTATGTCTAAGGTATAAGCGTGGGTTCTCTGCAAAAAACTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGTCAcc                                                                                                                                                     >  1:936565/2‑150 (MQ=255)
        aaaGGATTTATGTCTAAGGTATAAGCGTGGGTTCTCTGCAAAAAACTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGc                                                                                                                                               >  1:2864840/1‑150 (MQ=255)
        aaaGGATTTATGTCTAAGGTATAAGCGTGGGTTCTCTGCAAAAAACTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGc                                                                                                                                               <  1:1878565/150‑1 (MQ=255)
         aaGGATTTATGTCTAAGGTATAAGCGTGGGTTCTCTGCAAAAAACTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCa                                                                                                                                              <  1:2467389/150‑1 (MQ=255)
         aaGGATTTATGTCTAAGGTATAAGCGTGGGTTCTCTGCAAAAAACTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCa                                                                                                                                              >  1:2638709/1‑150 (MQ=255)
          aGGATTTATGTCTAAGGTATAAGCGTGGGTTCTCTGCAAAAAACTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCCGACACCGTCTGCAg                                                                                                                                             >  1:39916/1‑150 (MQ=255)
          aGGATTTATGTCTAAGGAATAAGCGTGGGTTCTCTGCAAAAAACTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAg                                                                                                                                             >  1:1102724/1‑150 (MQ=255)
           ggATTTATGTCTAAGGTATAAGCGTGGGTTCTCTGCAAAAAACTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCTGCAGACGGTGTCTGCGGa                                                                                                                                            <  1:1356899/150‑1 (MQ=255)
            gATTTATGTCTAAGGTATAAGCGTGGGTTCTCTGCAAAAAACTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGaa                                                                                                                                           <  2:946222/150‑1 (MQ=255)
              tttATGTCTAAGGTATAAGCGTGGGTTCTCTGCAAAAAACTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCTGCAGACGGTGTCTGCGGAAtt                                                                                                                                         <  2:2722838/150‑1 (MQ=255)
              tttATGTCTAAGGTATAAGCGTGGGTTCTCTGCAAAAAACTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAAtt                                                                                                                                         <  2:1637351/150‑1 (MQ=255)
                tATGTCTAAGGTATAAGCGTGGGTTCTCTGCAAAAAACTTACGCCAAACGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCTGCAGACGGTGTCTGCGGAATTcc                                                                                                                                       <  2:337829/150‑1 (MQ=255)
                        aGGTATAAGCGTGGGTTCTCTGCAAAAAACTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGACTTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCa                                                                                                                               >  1:1322246/1‑150 (MQ=255)
                            ataAGCGTGGGTTCTCTGCAAAAAACTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGa                                                                                                                           >  2:1555171/1‑150 (MQ=255)
                            ataAGCGTGGGTTCTCTGCAAAAAACTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGa                                                                                                                           >  1:3061933/1‑150 (MQ=255)
                            ataAGCGTGGGTTCTCTGCAAAAAACTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGa                                                                                                                           >  2:3052217/1‑150 (MQ=255)
                               aGCTTGGGTTCTCTGCAAAAAACTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGAttt                                                                                                                        <  1:2957211/150‑1 (MQ=255)
                               aGCGTGGTTTCTCTGCAAAAAACTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCTTCTGCAGATTTCCCATCGGCAAAGAttt                                                                                                                        <  1:565991/150‑1 (MQ=255)
                                gCGTGGGTTCTCTGCAAAAAACTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATACACCAGACATTGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTg                                                                                                                       <  1:2387732/150‑1 (MQ=255)
                                             gCAAAAAACTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTcg                                                                                                          >  1:2602414/1‑149 (MQ=255)
                                             gCAAAAAACTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCt                                                                                                          >  1:2602728/1‑150 (MQ=255)
                                              cAAAAAAATAACTCCAAATGAGGCTTTTTTACCTCTTTTGTCAACATGTTTAAAATCACCAGACAATGTCTGGCGAATTAACACCAATTGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAAAATTTGCAGACGCGATCTg                                                                                                         >  2:1406367/1‑150 (MQ=255)
                                               aaaaaaCTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGc                                                                                                        >  2:1348128/1‑150 (MQ=255)
                                               aaaaaaCTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGc                                                                                                        >  2:1578856/1‑150 (MQ=255)
                                                   aaCTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCaaa                                                                                                    <  1:2608988/150‑1 (MQ=255)
                                                   aaCTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACAGCGTCTGCCaaa                                                                                                    <  2:1938895/150‑1 (MQ=255)
                                                        aCGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCttt                                                                                               >  2:2261528/1‑150 (MQ=255)
                                                        aCGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCGGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCttt                                                                                               <  2:467057/150‑1 (MQ=255)
                                                          gCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTcc                                                                                             >  2:928156/1‑150 (MQ=255)
                                                          gCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTcc                                                                                             >  2:1640608/1‑150 (MQ=255)
                                                            cAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTcccc                                                                                           <  2:3018939/150‑1 (MQ=255)
                                                            cAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTcccc                                                                                           >  2:1165231/1‑150 (MQ=255)
                                                                    gCTTTTTTACCTCTTTTTTAAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGAATAATACCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAg                                                                                   <  1:2596957/150‑1 (MQ=255)
                                                                     cTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAgg                                                                                  <  2:307181/150‑1 (MQ=255)
                                                                       tttttACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCCGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTc                                                                                >  2:1940135/1‑150 (MQ=255)
                                                                       tttttACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTc                                                                                >  2:179848/1‑150 (MQ=255)
                                                                       tttttACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTc                                                                                >  2:1913643/1‑150 (MQ=255)
                                                                         tttACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCa                                                                              >  2:1446421/1‑150 (MQ=255)
                                                                            aCCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCt                                                                           >  1:855168/1‑150 (MQ=255)
                                                                              ctctTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTAc                                                                         >  1:2195898/1‑150 (MQ=255)
                                                                              ctctTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTAc                                                                         >  1:397878/1‑150 (MQ=255)
                                                                              ctctTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTGTCCCCTTGCCCAGGTCCACCTAc                                                                         >  1:1247399/1‑150 (MQ=255)
                                                                              ctctTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATAGGCAAAGAATTGGGAGACGCTGTCTGCCAAATCGTTCCCCTTGCCCAGGTCCACCTAc                                                                         >  1:3004755/1‑150 (MQ=255)
                                                                               tctTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACg                                                                        >  1:2128348/1‑150 (MQ=255)
                                                                                      tCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGttt                                                                 >  1:2737150/1‑150 (MQ=255)
                                                                                        aaCATGTTGAAATTCACCAGACAATGTCGGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTAc                                                               <  1:1142011/150‑1 (MQ=255)
                                                                                           aTGTTGAAATTCACCAGACAATGTCTGGCGAATTAAAACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTACCCCTTGCCCAGGTCCACCTACCTCCGTTTACttt                                                            >  1:2902583/1‑150 (MQ=255)
                                                                                            tGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTc                                                           <  2:2638709/150‑1 (MQ=255)
                                                                                              ttGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCg                                                         >  1:2756037/1‑150 (MQ=255)
                                                                                                    ttCGCCAGACATTGTCTGGTGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTaaaa                                                   >  2:221823/1‑150 (MQ=255)
                                                                                                     tCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTaaaaa                                                  <  1:710078/150‑1 (MQ=255)
                                                                                                     tCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGGTTACTTTCCGGTaaaaa                                                  >  1:1389077/1‑150 (MQ=255)
                                                                                                       aCCAGACACTGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACg                                                <  1:3110846/150‑1 (MQ=255)
                                                                                                       aCCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACg                                                >  1:339996/1‑150 (MQ=255)
                                                                                                       aCCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACg                                                >  1:2075392/1‑150 (MQ=255)
                                                                                                       aCCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACg                                                <  2:1291240/150‑1 (MQ=255)
                                                                                                       aCCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACg                                                <  2:1291655/150‑1 (MQ=255)
                                                                                                       aCCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACg                                                >  1:519405/1‑150 (MQ=255)
                                                                                                       aCCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCACACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCGACGTCCGTTTACTTTACGTTAAAAACg                                                >  1:1882129/1‑150 (MQ=255)
                                                                                                         cAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAATGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCt                                              >  2:1171108/1‑150 (MQ=255)
                                                                                                          aGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAAGGCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGGCCGTTTACTTTCCGTTAAAAACGCTg                                             >  2:1561852/1‑150 (MQ=255)
                                                                                                           gACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGAAGCTGTCTGCCAAATCTTTCCCCGTGCCCAGGTCCACCAACGTCCGTTTACTTTCCGTTAAAAACGCTGa                                            >  1:76030/1‑150 (MQ=255)
                                                                                                            aCAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGAc                                           <  2:1871071/150‑1 (MQ=255)
                                                                                                            aCAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGAc                                           >  1:2985067/1‑150 (MQ=255)
                                                                                                            aCAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGAc                                           >  2:2942956/1‑150 (MQ=255)
                                                                                                                  tCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGc                                     >  1:434709/1‑150 (MQ=255)
                                                                                                                  tCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCGGTCTGCCAAATCTTTCCCCGTGCCCCGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGc                                     >  1:434773/1‑150 (MQ=255)
                                                                                                                    tGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAg                                   >  1:2015903/1‑150 (MQ=255)
                                                                                                                      gCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCt                                 >  1:2261630/1‑150 (MQ=255)
                                                                                                                      gCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCGTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAAAGCTGACGCCCGCCGCt                                 >  1:3068897/1‑150 (MQ=255)
                                                                                                                             aaCACCATTTGGAAGACCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTACGTTAAAAACGCTGACGCCCGCAGCTTTTATGa                          <  1:2584676/150‑1 (MQ=255)
                                                                                                                             aaCACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGa                          <  2:660893/150‑1 (MQ=255)
                                                                                                                             aaCACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGa                          >  2:504545/1‑150 (MQ=255)
                                                                                                                             aaCACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGa                          <  2:1300034/150‑1 (MQ=255)
                                                                                                                             aaCACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACCCTGTATGCCAAATCTTTCCCCATGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAAAGCTGACGCCCGCAGCTTTTATGa                          >  1:1779670/1‑150 (MQ=255)
                                                                                                                              acacCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGaa                         >  2:1805224/1‑150 (MQ=255)
                                                                                                                                 ccATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGCTTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGaaaaa                      <  2:2403014/150‑1 (MQ=255)
                                                                                                                                 ccATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAACTCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGaaaaa                      <  2:2348085/150‑1 (MQ=255)
                                                                                                                                 ccATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGaaaaa                      <  2:272774/150‑1 (MQ=255)
                                                                                                                                 ccATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGaaaaa                      <  2:2018605/150‑1 (MQ=255)
                                                                                                                                    ttGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAATTTTTTGCAGACGCTGGCAGCCAAATCATTCCCCTTGCCCCGGTCCACCAACCTACGTTTACATTCCGTTAAAAACCCTGACGCCCCCAGCGGTTATGTAAAtgag                   >  1:603095/1‑150 (MQ=255)
                                                                                                                                    ttGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGTTTTTGCAGACGCTGTCTGCCAAATATTTACCCGTGCCCAGGTCCACCAACGTCCGTTTACGTTCCGTTAAAAAAGCTGACGCCCGCAGCTTTTATGAAAAagag                   >  1:366353/1‑150 (MQ=255)
                                                                                                                                        gAATTCTGCAGACGGTGTCTGCGGAATTCCCATCGGCAAAGATTTGGCAGACGGGGTCTGCCAAAACTTTACCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAAAGCTGACGCCCGCAGCTTTTATGTAAAATAGACGc               >  1:2093486/1‑150 (MQ=255)
                                                                                                                                        gAATTCTGCAGACGGTGTCTGCGGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGGTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTGTTATGAAAAAGTGACGc               >  1:2093542/1‑150 (MQ=255)
                                                                                                                                           ttCTGCAGACGGTGTCTGCGGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGAAAAAGAGACGCTcc            >  2:1597023/1‑150 (MQ=255)
                                                                                                                                           ttCTGCAGACGGTGTCTGCGGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGAAAAAGAGACGCTcc            >  2:1954574/1‑150 (MQ=255)
                                                                                                                                            tcagCAGACACCGTCAGCAGAATTCCCATCGGCAAAGATTTGTCAGACGCTGTCTGCCAAATCGTTCCCCTTGCCCAGGTCCACCTACGTCCGATTAAATTACGTTAAAAACCCTGACCCCCCCAGCTTTTAATAAAAAGAGACGCTcag           >  2:1341199/4‑148 (MQ=255)
                                                                                                                                            tCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAATATTTGTCAGACGCTGTATGCCAAATCTTTACCCTTGCCCAGGACCACCTACGTCCGTTTACTTTAAGTTAAAAAACCTGACGCCCGCAGCATTTATGAAAAAGAGACGCTccc           >  2:1341197/1‑149 (MQ=255)
                                                                                                                                             ccGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTTGCAGACGCTGTCTGCCAAATCTTTCCCCTTTCCCAGGTCCACCTACGTCCGTTTACTTTACGTTAAAAAAGCTGACCCCCGCAGCTTTTATGAAAAAGAGACGCTCCGc          >  1:2516191/1‑150 (MQ=255)
                                                                                                                                             ccGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGAAAAAGGGACGCTCCGc          >  2:2458247/1‑150 (MQ=255)
                                                                                                                                              cGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGAAAAAGAGACGCTCCGCt         >  2:1951838/1‑150 (MQ=255)
                                                                                                                                              cGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTAATTTCCGTTAAAAACGCTGACGCCCGCAGCTGTTATGAAAAAGAGACGCTCCGCt         >  2:377581/1‑150 (MQ=255)
                                                                                                                                               gCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCGGTCTGCCAAATCTTTCCCCGTGCCCAGGTCCCCCAACGGCCGGTTACGTTCCGGTAAAACGCTGACGCCCCCAGCGGTTATGTAAAAGAGACGcgccgcggg       >  1:947676/1‑147 (MQ=255)
                                                                                                                                                cAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTGGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGAAAAAGAGACGCTCCGCtgt       <  2:2334380/150‑1 (MQ=255)
                                                                                                                                                 cgACACCGTCGGCAGAAGTCCCATCGGCAAAGATGTGGCAGACGCTGTCTGCCAAATCTTTCCCCTGGCCCAGGTCCACCTACGTCCGTTTCCTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGAAAAAGAGACGCTCCGCtgtg      <  1:176824/149‑1 (MQ=255)
                                                                                                                                                 aGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCTTTAAAAACGCTGACGCCCGCAGCTTTTATGAAAAAGAGACGCTCCGCtgtg      <  1:674337/150‑1 (MQ=255)
                                                                                                                                                 aGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGAAAAAGAGACGCTCCGCtgtg      <  1:2025034/150‑1 (MQ=255)
                                                                                                                                                 aGACACCGTCTGAAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTGGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGAAAAAGAGACGCTCCGCtgtg      <  1:176753/150‑1 (MQ=255)
                                                                                                                                                     acCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGAAAAAGAGACGCTCCGCTGTGGCTg  <  1:352736/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
CGACTATCAAAGGATTTATGTCTAAGGTATAAGCGTGGGTTCTCTGCAAAAAACTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGAAAAAGAGACGCTCCGCTGTGGCTG  >  NC_000913/3368031‑3368329

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: