Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,368,189 A→G 5.3% A121A (GCA→GCG yhcG → DUF1016 family protein in the PD‑(D/E)XK nuclease superfamily

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,368,1890AG 5.3% 426.5 / 9.4 131A121A (GCA→GCGyhcGDUF1016 family protein in the PD‑(D/E)XK nuclease superfamily
Reads supporting (aligned to +/- strand):  ref base A (75/49);  new base G (4/3);  total (79/52)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.18e-01

Only 100 of 137 total aligned reads displayed.

AAGGATTTATGTCTAAGGTATAAGCGTGGGTTCTCTGCAAAAAACTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGAAAAAGAGACGCTCCGCTGTGGCTGGTCTGTTCG  >  NC_000913/3368040‑3368338
                                                                                                                                                     |                                                                                                                                                     
aaGGATTTATGTCTAAGGTATAAGCGTGGGTTCTCTGCAAAAAACTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCa                                                                                                                                                       >  1:308683/1‑150 (MQ=255)
aaGGATTTATGTCTAAGGTATAAGCGTGGGTTCTCTGCAAAAAACTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCa                                                                                                                                                       <  2:1582622/150‑1 (MQ=255)
aaGGATTTATGTCTAAGGTATAAGCGTGGGTTCTCTGCAAAAAACTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCa                                                                                                                                                       <  1:2467389/150‑1 (MQ=255)
 aGGATTTATGTCTAAGGTATAAGCGTGGGTTCTCTGCAAAAAACTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCCGACACCGTCTGCAg                                                                                                                                                      >  1:39916/1‑150 (MQ=255)
 aGGATTTATGTCTAAGGAATAAGCGTGGGTTCTCTGCAAAAAACTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAg                                                                                                                                                      >  1:1102724/1‑150 (MQ=255)
  ggATTTATGTCTAAGGTATAAGCGTGGGTTCTCTGCAAAAAACTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCTGCAGACGGTGTCTGCGGa                                                                                                                                                     <  1:1356899/150‑1 (MQ=255)
   gATTTATGTCTAAGGTATAAGCGTGGGTTCTCTGCAAAAAACTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGaa                                                                                                                                                    <  2:946222/150‑1 (MQ=255)
     tttATGTCTAAGGTATAAGCGTGGGTTCTCTGCAAAAAACTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAAtt                                                                                                                                                  <  2:1637351/150‑1 (MQ=255)
     tttATGTCTAAGGTATAAGCGTGGGTTCTCTGCAAAAAACTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAAtt                                                                                                                                                  <  1:770383/150‑1 (MQ=255)
       tATGTCTAAGGTATAAGCGTGGGTTCTCTGCAAAAAACTTACGCCAAACGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCTGCAGACGGTGTCTGCGGAATTcc                                                                                                                                                <  2:337829/150‑1 (MQ=255)
        aTGTCTAAGGTATAAGCGTGGGTTCTCTGCAAAAAACTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTccc                                                                                                                                               >  1:518623/1‑150 (MQ=255)
             tAAGGTATAAGCGTGGGTTCTCTGCAAAAAACTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCgg                                                                                                                                          <  2:1262094/150‑1 (MQ=255)
               aGGTATAAGCGTGGGTTCTCTGCAAAAAACTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGACTTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCa                                                                                                                                        >  1:1322246/1‑150 (MQ=255)
               aGGTATAAGCGTGGGTTCTCTGCAAAAAACTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCa                                                                                                                                        >  1:1824670/1‑150 (MQ=255)
                   ataAGCGTGGGTTCTCTGCAAAAAACTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGa                                                                                                                                    >  2:3052217/1‑150 (MQ=255)
                      aGCGTGGTTTCTCTGCAAAAAACTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCTTCTGCAGATTTCCCATCGGCAAAGAttt                                                                                                                                 <  1:565991/150‑1 (MQ=255)
                       gCGTGGGTTCTCTGCAAAAAACTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATACACCAGACATTGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTg                                                                                                                                <  1:2387732/150‑1 (MQ=255)
                            ggTTCTCTGCAAAAAACTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGa                                                                                                                           <  1:2272800/150‑1 (MQ=255)
                                    gCAAAAAACTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACCCCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTcg                                                                                                                   >  1:2602420/1‑149 (MQ=255)
                                    gCAAAAAACTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCt                                                                                                                   >  1:2602728/1‑150 (MQ=255)
                                     cAAAAAAATAACTCCAAATGAGGCTTTTTTACCTCTTTTGTCAACATGTTTAAAATCACCAGACAATGTCTGGCGAATTAACACCAATTGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAAAATTTGCAGACGCGATCTg                                                                                                                  >  2:1406367/1‑150 (MQ=255)
                                      aaaaaaCTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGc                                                                                                                 >  2:1348128/1‑150 (MQ=255)
                                      aaaaaaCTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGc                                                                                                                 >  2:1578856/1‑150 (MQ=255)
                                          aaCTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCaaa                                                                                                             <  2:3066071/150‑1 (MQ=255)
                                          aaCTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCaaa                                                                                                             <  1:2608988/150‑1 (MQ=255)
                                          aaCTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACAGCGTCTGCCaaa                                                                                                             <  2:1938895/150‑1 (MQ=255)
                                               aCGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCttt                                                                                                        >  2:2261528/1‑150 (MQ=255)
                                               aCGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCGGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCttt                                                                                                        <  2:467057/150‑1 (MQ=255)
                                                 gCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTcc                                                                                                      >  2:1640608/1‑150 (MQ=255)
                                                 gCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTcc                                                                                                      >  2:928156/1‑150 (MQ=255)
                                                   cAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTcccc                                                                                                    >  2:1165231/1‑150 (MQ=255)
                                                    aaaTGAGGCTTTTTTACCTCTTTTTTCAACATGTTTAAATTCACCAGACACTGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCTGACGCTGTCTGCCAAATCTTTCCCCt                                                                                                   <  1:2089910/150‑1 (MQ=255)
                                                           gCTTTTTTACCTCTTTTTTAAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGAATAATACCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAg                                                                                            <  1:2596957/150‑1 (MQ=255)
                                                             ttttttACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGt                                                                                          >  1:272774/1‑150 (MQ=255)
                                                              tttttACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCCGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTc                                                                                         >  2:1940135/1‑150 (MQ=255)
                                                              tttttACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTc                                                                                         >  2:1913643/1‑150 (MQ=255)
                                                              tttttACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTc                                                                                         >  2:179848/1‑150 (MQ=255)
                                                                     ctctTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTAc                                                                                  >  1:2195898/1‑150 (MQ=255)
                                                                     ctctTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTAc                                                                                  >  1:397878/1‑150 (MQ=255)
                                                                     ctctTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTGTCCCCTTGCCCAGGTCCACCTAc                                                                                  >  1:1247399/1‑150 (MQ=255)
                                                                     ctctTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATAGGCAAAGAATTGGGAGACGCTGTCTGCCAAATCGTTCCCCTTGCCCAGGTCCACCTAc                                                                                  >  1:3004755/1‑150 (MQ=255)
                                                                             tCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGttt                                                                          >  1:2737150/1‑150 (MQ=255)
                                                                               aaCATGTTGAAATTCACCAGACAATGTCGGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTAc                                                                        <  1:1142011/150‑1 (MQ=255)
                                                                                  aTGTTGAAATTCACCAGACAATGTCTGGCGAATTAAAACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTACCCCTTGCCCAGGTCCACCTACCTCCGTTTACttt                                                                     >  1:2902583/1‑150 (MQ=255)
                                                                                   tGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTc                                                                    <  1:2562095/150‑1 (MQ=255)
                                                                                   tGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTc                                                                    <  2:2638709/150‑1 (MQ=255)
                                                                                           ttCGCCAGACATTGTCTGGTGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTaaaa                                                            >  2:221823/1‑150 (MQ=255)
                                                                                            tCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTaaaaa                                                           <  1:710078/150‑1 (MQ=255)
                                                                                            tCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGGTTACTTTCCGGTaaaaa                                                           >  1:1389077/1‑150 (MQ=255)
                                                                                              aCCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGTCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACg                                                         >  2:1306722/1‑150 (MQ=255)
                                                                                              aCCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACg                                                         >  1:339996/1‑150 (MQ=255)
                                                                                              aCCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACg                                                         <  2:1291240/150‑1 (MQ=255)
                                                                                              aCCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCACACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCGACGTCCGTTTACTTTACGTTAAAAACg                                                         >  1:1882129/1‑150 (MQ=255)
                                                                                                cAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCt                                                       >  2:1510573/1‑150 (MQ=255)
                                                                                                 aGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAAGGCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGGCCGTTTACTTTCCGTTAAAAACGCTg                                                      >  2:1561852/1‑150 (MQ=255)
                                                                                                  gACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGa                                                     >  2:1133994/1‑150 (MQ=255)
                                                                                                  gACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGAAGCTGTCTGCCAAATCTTTCCCCGTGCCCAGGTCCACCAACGTCCGTTTACTTTCCGTTAAAAACGCTGa                                                     >  1:76030/1‑150 (MQ=255)
                                                                                                   aCAATGTCTGGCGAATTAACTCCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGAGTTGGCAGACGCGGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTTCGTCCGTTTGCTTTCCGTTTAAAACGCTGAc                                                    >  2:2943070/1‑150 (MQ=255)
                                                                                                   aCAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGAc                                                    >  2:2942956/1‑150 (MQ=255)
                                                                                                   aCAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGAc                                                    <  2:1871071/150‑1 (MQ=255)
                                                                                                   aCAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGAc                                                    >  1:2985067/1‑150 (MQ=255)
                                                                                                         tCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCGGTCTGCCAAATCTTTCCCCGTGCCCCGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGc                                              >  1:434773/1‑150 (MQ=255)
                                                                                                           tGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAg                                            >  1:2015903/1‑150 (MQ=255)
                                                                                                             gCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCGTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAAAGCTGACGCCCGCCGCt                                          >  1:3068897/1‑150 (MQ=255)
                                                                                                             gCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCGGCCAAATCTTTCCCCGTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCt                                          >  2:1372013/1‑150 (MQ=255)
                                                                                                                    aaCACCATTTGGAAGACCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTACGTTAAAAACGCTGACGCCCGCAGCTTTTATGa                                   <  1:2584676/150‑1 (MQ=255)
                                                                                                                    aaCACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGa                                   <  2:660893/150‑1 (MQ=255)
                                                                                                                    aaCACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGa                                   >  2:504545/1‑150 (MQ=255)
                                                                                                                    aaCACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGa                                   <  2:1300034/150‑1 (MQ=255)
                                                                                                                    aaCACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACCCTGTATGCCAAATCTTTCCCCATGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAAAGCTGACGCCCGCAGCTTTTATGa                                   >  1:1779670/1‑150 (MQ=255)
                                                                                                                     acacCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGaa                                  >  2:1805224/1‑150 (MQ=255)
                                                                                                                     acacCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCATGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACCCCCGCAGCTTTTATGaa                                  >  2:2707917/1‑150 (MQ=255)
                                                                                                                       acCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAATGAATTTGCAGTCGCGGTCTGCCAAATCTTTACCCGTGCCCAGGTCCACCTACCTCCGTTTTCTTTGCGTTTAAATCGCTGACGCCCGCAGCTGTTATGaaaa                                >  1:2145574/1‑150 (MQ=255)
                                                                                                                        ccATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAACTCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGaaaaa                               <  2:2348085/150‑1 (MQ=255)
                                                                                                                        ccATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGaaaaa                               <  2:2018605/150‑1 (MQ=255)
                                                                                                                        ccATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGaaaaa                               <  2:272774/150‑1 (MQ=255)
                                                                                                                           ttGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAATTTTTTGCAGACGCTGGCAGCCAAATCATTCCCCTTGCCCCGGTCCACCAACCTACGTTTACATTCCGTTAAAAACCCTGACGCCCCCAGCGGTTATGTAAAtgag                            >  1:603095/1‑150 (MQ=255)
                                                                                                                               gAATTCTGCAGACGGTGTCTGCGGAATTCCCATCGGCAAAGATTTGGCAGACGGGGTCTGCCAAAACTTTACCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAAAGCTGACGCCCGCAGCTTTTATGTAAAATAGACGc                        >  1:2093486/1‑150 (MQ=255)
                                                                                                                               gAATTCTGCAGACGGTGTCTGCGGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGGTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTGTTATGAAAAAGTGACGc                        >  1:2093542/1‑150 (MQ=255)
                                                                                                                                  ttCTGCAGACGGTGTCTGCGGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGAAAAAGAGACGCTcc                     >  2:1597023/1‑150 (MQ=255)
                                                                                                                                  ttCTGCAGACGGTGTCTGCGGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGAAAAAGAGACGCTcc                     >  2:1954574/1‑150 (MQ=255)
                                                                                                                                   tCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAATATTTGTCAGACGCTGTATGCCAAATCTTTACCCTTGCCCAGGACCACCTACGTCCGTTTACTTTAAGTTAAAAAACCTGACGCCCGCAGCATTTATGAAAAAGAGACGCTccc                    >  2:1341197/1‑149 (MQ=255)
                                                                                                                                    ccGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGAAAAAGGGACGCTCCGc                   >  2:2458247/1‑150 (MQ=255)
                                                                                                                                     cGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGAAAAAGAGACGCTCCGCt                  >  2:1951838/1‑150 (MQ=255)
                                                                                                                                     cGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTAATTTCCGTTAAAAACGCTGACGCCCGCAGCTGTTATGAAAAAGAGACGCTCCGCt                  >  2:377581/1‑150 (MQ=255)
                                                                                                                                      gCAGACACCGTCTGCAGAATTCCCATCGGCAATTATTTTGCAGACGCTGTCTGCCAAATCTTTACCCATTCCCAGGTCCACCTAAGTCCGTTTAATTTCAGGTAAAAAAGCAGAAGCCCGCAGCTTTTATGTAAAAGAGACGCGCCGCtg                 >  1:647726/1‑150 (MQ=255)
                                                                                                                                      gCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTACCCTTGCCCAGGTCCACCAACGTCCGTTTACATTACGTTAAAAAAGCTTACGCCCGCAGCTTTTATGAAAAAGAGACGCTCCGCtg                 >  1:2964783/1‑150 (MQ=255)
                                                                                                                                      gCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCGGTCTGCCAAATCTTTCCCCGTGCCCAGGTCCCCCAACGGCCGGTTACGTTCCGGTAAAACGCTGACGCCCCCAGCGGTTATGTAAAAGAGACGcgccgcggg                >  1:947676/1‑147 (MQ=255)
                                                                                                                                       cAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTGGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGAAAAAGAGACGCTCCGCtgt                <  2:2334380/150‑1 (MQ=255)
                                                                                                                                        cgACACCGTCGGCAGAAGTCCCATCGGCAAAGATGTGGCAGACGCTGTCTGCCAAATCTTTCCCCTGGCCCAGGTCCACCTACGTCCGTTTCCTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGAAAAAGAGACGCTCCGCtgtg               <  1:176824/149‑1 (MQ=255)
                                                                                                                                        aGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGAAAAAGAGACGCTCCGCtgtg               <  1:2025034/150‑1 (MQ=255)
                                                                                                                                        aGACACCGTCTGAAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTGGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGAAAAAGAGACGCTCCGCtgtg               <  1:176753/150‑1 (MQ=255)
                                                                                                                                           cacCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGAAAAAGAGACGCTCCGCTGTGGCt            <  1:2783975/150‑1 (MQ=255)
                                                                                                                                            acCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGAAAAAGAGACGCTCCGCTGTGGCTg           <  1:352736/150‑1 (MQ=255)
                                                                                                                                            acCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGAAAAAGAGACGCTCCGCTGTGGCTg           <  1:352840/150‑1 (MQ=255)
                                                                                                                                            acCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCGGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGAAAAAGAGACGCTCCGCTGTGGCTg           <  1:422240/150‑1 (MQ=255)
                                                                                                                                             ccGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGAAAAAGAGACGCTCCGCTGTGGCTgg          >  2:1542580/1‑150 (MQ=255)
                                                                                                                                              cGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGAAAAAGAGACGCTCCGCTGTGGCTGGt         <  1:2824912/150‑1 (MQ=255)
                                                                                                                                                  tGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGAAAAAGAGACGCTCCGCTGTGGCTGGTCTGt     >  2:3111559/1‑150 (MQ=255)
                                                                                                                                                   gCAGAATTCCCATCGGCCAAGATGTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGAAAAAGAGACGCTCCGCTGTGGCTGGTCTGtt    <  1:1940135/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
AAGGATTTATGTCTAAGGTATAAGCGTGGGTTCTCTGCAAAAAACTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGAAAAAGAGACGCTCCGCTGTGGCTGGTCTGTTCG  >  NC_000913/3368040‑3368338

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: