Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,460,074 T→A 8.6% G86G (GGT→GGA)  gspF → general secretory pathway component, cryptic

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,460,0740TA 8.6% 562.4 / 3.3 177G86G (GGT→GGA) gspFgeneral secretory pathway component, cryptic
Reads supporting (aligned to +/- strand):  ref base T (107/52);  new base A (2/15);  total (109/68)
Fisher's exact test for biased strand distribution p-value = 1.14e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 183 total aligned reads displayed.

TTCCTGCTGGATATTCGCCCCCAAAAAAGTTCGGGAGTAAAAACACGTCGCCCGAGGATCAGCCATAGTGAACTGACGCTTTTCACCCGGCAGTTGGCAACCTTAAGCGCAGCGGCATTACCCCTGGAAGAGAGCCTTGCCGTAATCGGTCAACAAAGCAGTAATAAACGACTGGGTGACGTGTTAAATCAGGTACGCAGCGCCATCCTTGAAGGGCATCCCCTTTCCGATGCATTACAGCATTTTCCCACGCTTTTCGATTCGCTCTATCGTACCCTGGTAAAAGCGGGCGAAAAGAG  >  NC_000913/3459925‑3460223
                                                                                                                                                     |                                                                                                                                                     
ttCCTGCTGGATATTCGCCCCCAAAAAAGTTCGGGAGTAAAAACACGTCGCCCGAGGATCAGCCATAGTGAACTGACGCTTTTCACCCGGCAGTTGGCAACCTTAAGCGCAGCGGCATTACCCCTGGAAGAGAGCCTTGCCGTAATCGGt                                                                                                                                                       <  1:2151821/150‑1 (MQ=255)
ttCCTGCTGGATATTCGCCCCCAAAAAAGTTCGGGAGTAAAAACACGTCGCCCGAGGATCAGCCATAGTGAACTGACGCTTTTCACCCGGCAGTTGGCAACCTTAAGCGCAGCGGCATTACCCCTGGAAGAGAGCCTTGCCGTAATCGGt                                                                                                                                                       <  2:3002158/150‑1 (MQ=255)
ttCCTGCTGGATATTCGCCCCCAAAAAAGTTCGGGAGTAAAAACACGTCGCCCGAGGATCAGCCATAGTGAACTGACGCTTTTCACCCGGCAGTTGGCAACCTTAAGCGCAGCGGCATTACCCCTGGAAGAGAGCCTTGCCGTAATCGGt                                                                                                                                                       <  1:35761/150‑1 (MQ=255)
   ctgctgGATATTCGCCCCCAAAAAAGTTCGGGAGTAAAAACACGTCGCCCGAGGATCAGCCATAGTGAACTGACGCTTTTCACCCGGCAGTTGGCAACCTTAAGCGCAGCGGCATTACCCCTGGAAGAGAGCCTTGCCGTAATCGGTcaa                                                                                                                                                    <  2:91627/150‑1 (MQ=255)
    tgctgGATATTCGCCCCCAAAAAAGTTCGGGAGTAAAAACACGTCGCCCGAGGATCAGCCATAGTGAACTGACGCTTTTCACCCGGCAGTTGGCAACCTTAAGCGCAGCGGCATTACCCCTGGAAGAGAGCCTTGCCGTAATCGGTcaac                                                                                                                                                   <  2:145187/150‑1 (MQ=255)
    tgctgGATATTCGCCCCCAAAAAAGTTCGGGAGTAAAAACACGTCGCCCGAGGATCAGCCATAGTGAACTGACGCTTTTCACCCGGCAGTTGGCAACCTTAAGCGCAGCGGCATTACCCCTGGAAGAGAGCCTTGCCGTAATCGGTcaac                                                                                                                                                   >  1:1076423/1‑150 (MQ=255)
    tgctgGATATTCGCCCCCAAAAAAGTTCGGGAGTAAAAACACGTCGCCCGAGGATCAGCCATAGTGAACTGACGCTTTTCACCCGGCAGTTGGCAACCTTAAGCGCAGCGGCATTACCCCTGGAAGAGAGCCTTGCCGTAATCGGTcaac                                                                                                                                                   >  1:2261703/1‑150 (MQ=255)
      ctgGATATTCGCCCCCAAAAAAGTTCGGGAGTAAAAACACGTCGCCCGAGGATCAGCCATAGTGAACTGACGCTTTTCACCCGGCAGTTGGCAACCTTAAGCGCAGCGGCATTACCCCTGGAAGAGAGCCTTGCCGTAATCGGTcaacaa                                                                                                                                                 >  2:7256/1‑150 (MQ=255)
      ctgGATATTCGCCCCCAAAAAAGTTCGGGAGTAAAAACACGTCGCCCGAGGATCAGCCATAGTGAACTGACGCTTTTCACCCGGCAGTTGGCAACCTTAAGCGCAGCGGCATTACCCCTGGAAGAGAGCCTTGCCGTAATCGGTcaacaa                                                                                                                                                 >  2:2651504/1‑150 (MQ=255)
        ggATATTCGCCCCCAAAAAAGTTCGGGAGTAAAAACCCGTCGCCCGAGGATCAGCCATAGTGAACTGACGCTTTTCACCCGGCAGTTGGCAACCTTAAGCGCAGCGGCATTACCCCTGGAAGAGAGCCTTGCCGTAATCGGTCAACAAAg                                                                                                                                               <  2:1070114/150‑1 (MQ=255)
        ggATATTCGCCCCCAAAAAAGTTCGGGAGTAAAAACACGTCGCCCGAGGATCAGCCATAGTGAACTGACGCTTTTCACCCGGCAGTTGGCAACCTTAAGCGCAGCGGCATTACCCCTGGAAGAGAGCCTTGCCGTAATCGGTCAACAAAg                                                                                                                                               <  1:1270964/150‑1 (MQ=255)
        ggATATTCGCCCCCAAAAAAGTTCGGGAGTAAAAACACGTCGCCCGAGGATCAGCCATAGTGAACTGACGCTTTTCACCCGGCAGTTGGCAACCTTAAGCGCAGCGGCATTACCCCTGGAAGAGAGCCTTGCCGTAATCGGTCAACAAAg                                                                                                                                               >  1:498850/1‑150 (MQ=255)
         gATATTCGCCCCCAAAAAAGTTCGGGAGTAAAAACACGTCGCCCGAGGATCAGCCATAGTGAACTGACGCTTTTCACCCGGCAGTTGGCAACCTTAAGCGCAGCGGCATTACCCCTGGAAGAGAGCCTTGCCGTAATCGGTCAACAAAGc                                                                                                                                              >  2:2924076/1‑150 (MQ=255)
         gATATTCGCCCCCAAAAAAGTTCGGGAGTAAAAACACGTCGCCCGAGGATCAGCCATAGTGAACTGACGCTTTTCACCCGGCAGTTGGCAACCTTAAGCGCAGCGGCATTACCCCTGGAAGAGAGCCTTGCCGTAATCGGTCAACAAAGc                                                                                                                                              >  1:690778/1‑150 (MQ=255)
         gATATTCGCCCCCAAAAAAGTTCGGGAGTAAAAACACGTCGCCCGAGGATCAGCCATAGTGAACTGACGCTTTTCACCCGGCAGTTGGCAACCTTAAGCGCAGCGGCATTACCCCTGGAAGAGAGCCTTGCCGTAATCGGTCAACAAAGc                                                                                                                                              >  1:3053951/1‑150 (MQ=255)
         gATATTCGCCCCCAAAAAAGTTCGGGAGTAAAAACAAGTCGCCCGAGGATCAGAAATAGTGAAATGACGCTTTTCACACGGCAGTTGGCAACCTTAAGCGCAGAGGCATAACCCCTGTAAGAGAGCCTTGACTTAATCGGACAACAAAGc                                                                                                                                              >  1:2078938/1‑150 (MQ=255)
          atatTCGCCCCCAAAAAAGTTCGGGAGTAAAAACACGTCGCCCGAGGATCAGCCATAGTGAACTGACGCTTTTCACCCGGCAGTTGGCAACCTTAAGCGCAGCGGCATTACCCCTGGAAGAGAGCCTTGCCGTAATCGGTCAACAAAGCa                                                                                                                                             <  1:2442918/150‑1 (MQ=255)
             ttCGCCCCCAAAAAAGTTCGGGAGTAAAAACACGTCGCCCGAGGATCAGCCATAGTGAACTGACGCTTTTCACCCGGCAGTTGGCAACCTTAAGCGCAGCGGCATTACCCCTGGAAGAGAGCCTTGCCGTAATCGGTCAACAAAGCAGta                                                                                                                                          <  1:1639167/150‑1 (MQ=255)
             ttCGCCCCCAAAAAAGTTCGGGAGTAAAAACACGTCGCCCGAGGATCAGCCATAGTGAACTGACGCTTTTCACCCGGCAGTTGGCAACCTTAAGCGCAGCGGCATTACCCCTGGAAGAGAGCCTTGCCGTAATCGGTCAACAAAGCAGta                                                                                                                                          <  2:889033/150‑1 (MQ=255)
              tCGCCCCCAAAAAAGATCGGGAGTAAAAACACGTCGCCCGAGGATCAGCCATAGTGAACTGACGCTTTTCACCCGGCAGTTGGCAACCTTAAGCGCAGCGGCATTACCCCTGGAAGAGAGCCTTGCCGTAATCGGTCAACAAAGCAGtaa                                                                                                                                         >  1:2878733/1‑150 (MQ=255)
                gCCCCCAAAAAAGTTCGGGAGTAAAAACACGTCGCCCGAGGATCAGCCATAGTGAACTGACGCTTTTCACCCGGCAGTTGGCAACCTTAAGCGCAGCGGCATTACCCCTGGAAGAGAGCCTTGCCGTAATCGGTCAACAAAGCAGtaata                                                                                                                                       <  2:826636/150‑1 (MQ=255)
                 cccccAAAAAAGTTCGGGAGTAAAAACACGTCGCCCGAGGATCAGCCATAGTGAACTGACGCTTTTCACCCGGCAGTTGGCAACCTTAAGCGCAGCGGCATTACCCCTGGAAGAGAGCCTTGCCGTAATCGGTCAACAAAGCAGtaataa                                                                                                                                      <  2:2388252/150‑1 (MQ=255)
                  ccccAAAAAAGTTCGGGAGTAAAAACACGTCGCCCGAGGATCAGCCATAGTGAACTGACGCTTTTCACCCGGCAGTTGGCAACCTTAAGCGCAGCGGCATTACCCCTGGAAGAGAGCCTTGCCGTAATCGGTCAACAAAGCAGTAATaaa                                                                                                                                     >  2:183812/1‑150 (MQ=255)
                   cccAAAAAAGTTCGGGAGTAAAAACACGTCGCCCGAGGATCAGCCATAGTGAACTGACGCTTTTCACCCGGCAGTTGGCAACCTTAAGCGCAGCGGCATTACCCCTGGAAGAGAGCCTTGCCGTAATCGGTCAACAAAGCAGTAATAAAc                                                                                                                                    >  1:1042642/1‑150 (MQ=255)
                   cccAAAAAAGTTCGGGAGTAAAAACACGTCGCCCGAGGATCAGCCATAGTGAACTGACGCTTTTCACCCGGCAGTTGGCAACCTTAAGCGCAGCGGCATTACCCCTGGAAGAGAGCCTTGCCGTAATCGGTCAACAAAGCAGTAATAAAc                                                                                                                                    >  1:320509/1‑150 (MQ=255)
                    ccAAAAAAGTTCGGGAGTAAAAACACGTCGCCCGAGGATCAGCCATAGTGAACTGACGCTTTTCACCCGGCAGTTGGCAACCTTAAGCGCAGCGGCATTACCCCTGGAAGAGAGCCTTGCCGTAATCGGTCAACAAAGCAGTAATAAACg                                                                                                                                   >  2:2125174/1‑150 (MQ=255)
                      aaaaaaGTTCGGGAGTAAAAACACGTCGCCCGAGGATCAGCCATAGTGAACTGACGCTTTTCACCCGGCAGTTGGCAACCTTAAGCGCAGCGGCATTACCCCTGGAAGAGAGCCTTGCCGTAATCGGTCAACAAAGCAGTAATAAACGAc                                                                                                                                 >  2:1387508/1‑150 (MQ=255)
                      aaaaaaGTTCGGGAGTAAAAACACGTCGCCCGAGGATCAGCCATAGTGAACTGACGCTTTTCACCCGGCAGTTGGCAACCTTAAGCGCAGCGGCATTACCCCTGGAAGAGAGCCTTGCCGTAATCGGTCAACAAAGCAGTAATAAACGAc                                                                                                                                 >  2:2447612/1‑150 (MQ=255)
                      aaaaaaGTTCGGGAGTAAAAACACGTCGCCCGAGGATCAGCCATAGTGAACTGACGCTTTTCACCCGGCAGTTGGCAACCTTAAGCGCAGCGGCATTACCCCTGGAAGAGAGCCTTGCCGTAATCGGTCAACAAAGCAGTAATAAACGAc                                                                                                                                 >  2:427447/1‑150 (MQ=255)
                            gTTCGGGAGTAAAAACACGTCGCCCGAGGATCAGCCATAGTGAACTGACGCTTTTCACCCGGCAGTTGGCAACCTTAAGCGCAGCGGCATTACCCCTGGAAGAGAGCCTTGCCGTAATCGGTCAACAAAGCAGTAATAAACGACTGGGTg                                                                                                                           >  2:3015337/1‑150 (MQ=255)
                             ttCGGGAGTAAAAACACGTCGCCCGAGGATCAGCCATAGTGAACTGACGCTTTTCACCCGGCAGTTGGCAACCTTAAGCGCAGCGGCATTACCCCTGGAAGAGAGCCTTGCCGTAATCGGTCAACAAAGCAGTAATAAACGACTGGGTGa                                                                                                                          >  1:1839156/1‑150 (MQ=255)
                                  gAGTAAAAACACGTCGCCCGAGGATCAGCCATAGTGAACTGACGCTTTTCACCCGGCAGTTGGCAACCTTAAGCGCAGCGGCATTACCCCTGGAAGAGAGCCTTGCCGTAATCGGTCAACAAAGCAGTAATAAACGACTGGGTGACgtgt                                                                                                                     >  2:1925340/1‑150 (MQ=255)
                                     tAAAAACACGTCGCCCGAGGATCAGCAATAGTGAACTGACGCTTTTCACCCGGCAGTTGGCAACCTTAAGCGCAGCGGCATTACCCCTGGAAGAGAGCCTTGCCGTAATCGGTCAACAAAGCAGTAATAAACGACTGGGTGACGTGTTaa                                                                                                                  <  2:1837766/150‑1 (MQ=255)
                                       aaaaCCCGTCGCCCGTGTATCTGCCATTGTGACCGGCCGCTTTTCACCCGGCTGTGGGCAACCTTACGCGCCGCGGCATTACCCCGGGACGAGCGCCTTGCCGTACTCGTTAAAAAACGCATTAATACACGACTGGGTGACGTGTTAAAt                                                                                                                <  2:1419566/150‑1 (MQ=255)
                                       aaaaCACGTCGCCCGAGTATCAGCAATAGTGAACTGACGCTTTTCACCCGGCTGTTGGCAACCTTATGCGCAGCGGCATTACCCCTGGAAGAGAGCCTTGCCGTAATCGGTCAACAAAGCAGTAATAAACGACTGGGTGACGTGTTAAAt                                                                                                                <  2:2525554/150‑1 (MQ=255)
                                       aaaaCACGTCGCCCGAGGATCAGCCATTGTGAACTGACGCTTTTCACCCGGCAGTTGGCAACCTTAAGCGCAGCGGCATTACCCCTGGAAGAGAGCCTTGCCGTAATCGGTCAACAAAGCAGTAATAAACGACTGGGTGACGTGTTAAAt                                                                                                                <  2:220936/150‑1 (MQ=255)
                                       aaaaCACGTCGCCCGAGGATCAGCCATAGTGAACTGACGCTTTTCACCCGGCAGTTGGCAACCTTAAGCGCAGCGGCATTACCCCTGGAAGAGAGCCTTGCCGTAATCGGTCAACAAAGCAGTAATAAACGACTGGGTGACGTGTTAAAt                                                                                                                >  2:2219669/1‑150 (MQ=255)
                                          acacGTCGCCCGAGGATCAGCCATAGTGAACTGACGCTTTACACCCGGAAGTTGGCACCCTTCAGCGCCGCGGCTTTACCCCTGTAAGAGAGCCTGGCCGTAATGGGTAAACAAAGCAGTAATAAACGACTGGGTGACGTGTTAAATCAg                                                                                                             <  2:1659865/150‑1 (MQ=255)
                                                        gATCAGCCATAGTGAACTGACGCTTTTCACCCGGCATATGGCAACCTTAAGCGCAGCGGCAATACCCCTGGAAGAGAGCAATGCCGTAATCGGTCAAAAAAGCAGTAATAAACGACTGGGTGACGTGTTAAATCAGGTACGCAGCGCCAt                                                                                               >  1:2549244/1‑150 (MQ=255)
                                                              ccATAGTGAACTGACGCTTTTCACCCGGCAGTTGGCAACCTTAAGCGCAGCGGCATTACCCCTGGAAGAGAGCCTTGCCGTAATCGGTCAACAAAGCAGTAATAAACGACTGGGTGACGTGTTAAATCAGGTACGCAGCGCCATCCTTGa                                                                                         >  2:1545600/1‑150 (MQ=255)
                                                                aTAGTGAACTGCCGCTTTTCACCGGGCTGTTGGAAACCTTACGCGCAGCGGCATTACCCCTGGACGAGAGCCTTGCCGTAATGGGTCAACAAAGCTGTAATAAACGACTGGGTGACGTGTTAAATCAGGTACGCAGCGCCATCCTTGAAg                                                                                       <  2:690778/150‑1 (MQ=255)
                                                                   gTGAACTGACGCTTTTCACCCGGCAGTTGGCAACCTTAAGCGCAGCGGCATTACCCCTGGAAGAGAGCCTTGCCGTAATCGGTCAACAAAGCAGTAATAAACGACTGGGTGACGTGTTAAATCAGGTACGCAGCGCCATCCTTGAAGGGc                                                                                    >  2:1233031/1‑150 (MQ=255)
                                                                   gTGAACTGACGCTTTTCACCCGGCAGTTGGCAACCTTAAGCGCAGCGGCATTACCCCTGGAAGAGAGCCTTGCCGTAATCGGTCAACAAAGCAGTAATAAACGACTGGGTGACGTGTTAAATCAGGTACGCAGCGCCATCCTTGAAGGGc                                                                                    >  1:579260/1‑150 (MQ=255)
                                                                          gACGCTTTTCACCCGGCAGTTGGCAACCTTAAGCGCAGCGGCATTACCCCTGGAAGAGAGCCTTGCCGTAATCGGTCAACAAAGCAGTAATAAACGACTGGGTGACGTGTTAAATCAGGTACGCAGCGCCATCCTTGAAGGGCATCCCCt                                                                             >  2:823672/1‑150 (MQ=255)
                                                                            cGCTTTTCACCCGGCAGTTGGCAACCTTAAGCGCAGCGGCATTCCCCCTGGAAGAGAGCCTTGCCTTAATCGGTCAACAAAGCAGTAATAAACGACTGGGTGACGTGTTAAATCAGGTACGCAGCGCCATCCTTGAAGGGCATCCCCttt                                                                           <  2:999062/150‑1 (MQ=255)
                                                                              cTTTCCCCCGGGCTGTTGCAAACGTTACGCGCCGCGCCATTCCCCCTGTAAGAGAGCCTTGCCTTAAGCGTACAACACCGAATTAATACACGACTGGGTGACGTGTTAAATCAGGTACGCAGCGCCATCCTTGAAGGGCATCCCCTTTcc                                                                         <  1:1255044/150‑1 (MQ=255)
                                                                                tttCACCCGGCAGTTGGCAACCTTAAGCGCAGCGGCATTACCCCTGGAAGAGAGCCTTGCCGTAATCGGTCAACAAAGCAGTAATAAACGACTGGGTGACGTGTTAAATCAGGTACGCAGCGCCATCCTTGAAGGGCATCCCCTTTCCGa                                                                       >  1:188961/1‑150 (MQ=255)
                                                                                ttacACCCGGCTGTTGGCACCCTTACGCGCCGCGGCATCCCCCCTGGAAGAGAGCCTTGCCGAAATCGTTCAAAAAAGAAGTAAAAAACGACTGGGTGACGTGTTAAATCAGGTACGTAGCGCCATCCTTGAAGGGCATCCCCTTTCCGa                                                                       <  1:2429111/147‑1 (MQ=255)
                                                                                 ttCCCCGGGCTGTTGGCAACCTTATGCGCTGGGGCTTTCCCCCTGGATGAGAGCGTGGCGGTATTGGGTAAACAACGCTTTAATACACGACTGGGTGACGTGTTAAATCAGGTACGCAGCGCCATCCTTGAAGGGCATCCCCTTTCCGAt                                                                      <  1:1737922/150‑1 (MQ=255)
                                                                                   cACCCGGCTGTTGGAAACCTTACGCGCAGCGGAATCACCCCTGTAAGAGAGCTTTGCCTTATTCGTACAACAACGCAGTAATACACGACTGGGTGACGTGTTAAATCAGGTACGCAGCGCCATCCTTGAAGGGCATCCCCTTTCCGATGc                                                                    <  1:8267/150‑1 (MQ=255)
                                                                                    aCCCGGCAGTTGGCAACCTTAAGCGCAGCGGCATTACCCCTGGAAGAGAGCCTTGCCGTAATCGGTCAACAAAGCAGTAATAAACGACTGGGTGACGTGTTAAATCAGGTACGCAGCGCCATCCTTGAAGGGCATCCCCTTTCCGATGCa                                                                   >  1:2772029/1‑150 (MQ=255)
                                                                                    aCCCGGCAGTTGGCAACCTTAAGCGCAGCGGCATTACCCCTGGAAGAGAGCCTTGCCGTAATCGGTCAACAAAGCAGTAATAAACGACTGGGTGACGTGTTAAATCAGGTACGCAGCGCCATCCTTGAAGGGCATCCCCTTTCCGATGCa                                                                   >  1:1780025/1‑150 (MQ=255)
                                                                                    aCCCGGCAGTTGGCAACCTTAAGCGCAGCGGCATTACCCCTGGAAGAGAGCCTTGCCGTAATCGGTCAACAAAGCAGTAATAAACGACTGGGTGACGTGTTAAATCAGGTACGCAGCGCCATCCTTGAAGGGCATCCCCTTTCCGATGCa                                                                   >  1:943387/1‑150 (MQ=255)
                                                                                    aCCCGGCAGTTGGCAACCTTAAGCGCAGCGGCATTACCCCTGGAAGAGAGCCTTGCCGTAATCGGTCAACAAAGCAGTAATAAACGACTGGGTGACGTGTTAAATCAGGTACGCAGCGCCATCCTTGAAGGGCATCCCCTTTCCGATGCa                                                                   >  1:852638/1‑150 (MQ=255)
                                                                                    aCCCGGAAGTTGGCAACCTTAAGCGCAGCGGCATTACCCCTGGAAGAGAGCCTTGCCGTAATCGGTCAACAAAGCAGTAATAAACGACTGGGTGACGTGTTAAATCAGGTACGCAGCGCCATCCTTGAAGGGCATCCCCTTTCCGATGCa                                                                   >  1:1780061/1‑150 (MQ=255)
                                                                                     cccGGCTGTTGGCAACATTAAGCGCCGCGTCATTACCCCTGGAATAGAGCCTTGCCGTAATCGGTCAACAAAGCAGTAATAAACGACTGGGTGACGTGTTAAATCAGGTACGCAGCGCCATCCTTGAAGGGCATCCCCTTTCCGATGCAt                                                                  <  1:2834333/150‑1 (MQ=255)
                                                                                      ccGGCAGTTGGCAACCTTAAGCGCAGCGGCATTACCCCTGGAAGAGAGCCTTGCCGTAATCGGTCAACAAAGCAGTAATAAACGACTGGGTGACGTGTTAAATCAGGTACGCAGCGCCATCCTTGAAGGGCATCCCCTTTCCGATGCAtt                                                                 >  1:191321/1‑150 (MQ=255)
                                                                                       cGGCAGTTGGCAACCTTAAGCGCAGCGGCATTACCCCTGGAAGAGAGCCTTGCCGTAATCGGTCACCAAAGCAGTAATAAACGACTCGGTGACGTGTTAAATCAGGAACGCAGCGCCATCCTTGAAGGGCATCCCCTTTCCGATGCATTa                                                                >  2:1767997/1‑150 (MQ=255)
                                                                                       cGGCAGTTGGCAACCTTAAGCGCAGCGGCATTACCCCTGGAAGAGAGCCTTGCCGTAATCGGTCAACAAAGCAGTAATAAACGACTGGGTGACGTGTTAAATCAGGTACGCAGCGCCATCCTTGAAGGGCATCCCCTTTCCGATGCATTa                                                                >  2:2897975/1‑150 (MQ=255)
                                                                                       cGGCAGTTGGCAACCTTAAGCGCAGCGGCATTACCCCTGGAAGAGAGCCTTGCCGTAATCGGTCAACAAAGCAGTAATAAACGACTGGGTGACGTGTTAAATCAGGTACGCAGCGCCATCCTTGAAGGGCATCCCCTTTCCGATGCATTa                                                                >  2:157362/1‑150 (MQ=255)
                                                                                           tgTTGGAAACCTTAAGCGCAGCGGCATTACCCCTGTAAGTGAGCCTTGCCTTAATCGTTAAACAAAGCATTAATAAACGACTGGGTGACGTGTTAAATCAGGTACGCAGCGCCATCCTTGAAGGGCATCCCCTTTCCGATGCATTACAGc                                                            <  2:365662/149‑1 (MQ=255)
                                                                                            gTTGGAAACCTTAAGGGCCGGGTAATTACCCCTGTATGAGCGCGTTGCCGTAATCGGACAACAAAGAATTAATAAACGACTGGGTGACGTGTTAAATCAGGTACGCAGCGCCATCCTTGAAGGGCATCCCCTTTCCGATGCATTACAGCa                                                           <  1:3082511/150‑1 (MQ=255)
                                                                                                gCAACCTTAAGCGCAGCGGCATTACCCCTGGAAGGGAGCCTTGCCGTAATCGGTCAACAAAGCAGTAATAAACGACTGGGTGACGTGTTAAATCAGGTACGCAGCGCCATCCTTGAAGGGCATCCCCTTTCCGATGCATTACAGCAtttt                                                       >  2:3029805/1‑150 (MQ=255)
                                                                                                gCAACCTTAAGCGCAGCGGCATTACCCCTGGAAGAGAGCCTTGCCGTAATCGGTCAACAAAGCAGTAATAAACGACTGGGTGACGTGTTAAATCAGGTACGCAGCGCCATCCTTGAAGGGCATCCCCTTTCCGATGCATTACAGCAtttt                                                       >  2:2169219/1‑150 (MQ=255)
                                                                                                  aaCCTTAAGCGCAGCGGCATTACCCCTGGAAGAGAGCCTTGCCGTAATCGGTCAACAAAGCAGTAATAAACGACTGGGTGACGTGTTAAATCAGGTACGCAGCGCCATCCTTGAAGGGCATCCCCTTTCCGATGCATTACAGCATTTTcc                                                     >  2:2356157/1‑150 (MQ=255)
                                                                                                  aaCCTTAAGCGCAGCGGCATTACCCCTGGAAGAGAGCCTTGCCGTAATCGGTCAACAAAGCAGTAATAAACGACTGGGTGACGTGTTAAATCAGGTACGCAGCGCCATCCTTGAAGGGCATCCCCTTTCCGATGCATTACAGCATTTTcc                                                     >  2:2356299/1‑150 (MQ=255)
                                                                                                    ccTTACGCGCCGCGGCATTCCCCCGGGACGAGAGCCTGGCCGTAATGGGTCAACAAAGCAGTAATACACGACTGGGTGACGTGTTAAATCAGGTACGCAGCGCCATCCTTGAAGGGCATCCCCTTTCCGATGCATTACAGCATTTTCCCa                                                   <  2:2713630/150‑1 (MQ=255)
                                                                                                    ccTTAAGCGCAGCGGCATTACCCCTGGAAGAGAGCCTTGCCGTAATCGGTCAACAAAGCAGTAATAAACGACTGGGTGACGTGTTAAATCAGGTACGCAGCGCCATCCTTGAAGGGCATCCCCTTTCCGATGCATTACAGCATTTTCCCa                                                   <  2:3053981/150‑1 (MQ=255)
                                                                                                    cattAAGCGCAGCGGAATAACCCCTGTAAGAGAGCCTTGCCGTAATCGGAAAAAAAAGCATTAATAAACGACTGGGTGACGTGTTAAATCAGGTACGCAGCGCCATCCTTGAAGGGCATCCCCTTTCCGATGCATTACAGCATTTTCCCa                                                   <  2:3053951/148‑1 (MQ=255)
                                                                                                      ttAAGCGCAGCGGCATTACCCCTGGAAGAGAGCCTTGCCGTAATCGGTCAACAAAGCAGTAATAAACGACTGGGTGACGTGTTAAATCAGGTACGCAGCGCCATCCTTGAAGGGCATCCCCTTTCCGATGCATTACAGCATTTTCCCACg                                                 >  1:378921/1‑150 (MQ=255)
                                                                                                      ttAAGCGCAGCGGCATTACCCCTGGAAGAGAGCCTTGCCGTAATCGGTCAACAAAGCAGTAATAAACGACTGGGTGACGTGTTAAATCAGGTACGCAGCGCCATCCTTGAAGGGCATCCCCTTTCCGATGCATTACAGCATTTTCCCACg                                                 >  1:985430/1‑150 (MQ=255)
                                                                                                       tacgcgcAGCGGCATTACCCCTGTAAGAGAGCCTTGCCTTAATCGGTCAACAAAGCATTAATAAACGACTGGGTGACGTGTTAAATCAGGTACGCAGCGCCATCCTTGAAGGGCATCCCCTTTCCGATGCATTACAGCATTTTCCCACGc                                                <  2:714375/147‑1 (MQ=255)
                                                                                                           cgcAGCGGCATTACCCCTGGAAGAGAGCCTTGCCGTAATCGGTCAACAAAGCAGTAATAAACGACTGGGTGACGTGTTAAATCAGGTACGCAGCGCCATCCTTGAAGGGCATCCCCTTTCCGATGCATTACAGCATTTTCCCACGCtttt                                            >  1:2593547/1‑150 (MQ=255)
                                                                                                            gcAGCGGCATTACCCCTGGAAGAGAGCCTTGCCGTAATCGGTCAACAAAGCAGTAATAAACGACTGGGTGACGTGTTAAATCAGGTACGCAGCGCCATCCTTGAAGGGCATCCCCTTTCCGATGCATTACAGCATTTTCCCACGCTTTTc                                           >  2:866583/1‑150 (MQ=255)
                                                                                                            gcAGCGGCATTACCCCTGGAAGAGAGCCTTGCCGTAATCGGTCAACAAAGCAGTAATAAACGACTGGGTGACGTGTTAAATCAGGTACGCAGCGCCATCCTTGAAGGGCATCCCCTTTCCGATGCATTACAGCATTTTCCCACGCTTTTc                                           >  2:866535/1‑150 (MQ=255)
                                                                                                            gcAGCGGCATTACCCCTGGAAGAGAGCCTTGCCGTAATCGGTCAACAAAGCAGTAATAAACGACTGGGTGACGTGTTAAATCAGGTACGCAGCGCCATCCTTGAAGGGCATCCCCTTTCCGATGCATTACAGCATTTTCCCACGCTTTTc                                           <  1:1528328/150‑1 (MQ=255)
                                                                                                               gCGGCTTTCCCCCTTTAAGAGAGCCTTGCCTTAATCTGTCAACAAAGCCTTAATAAACGACTGGGTGACGTGTTAAATCAGGTACGCAGCGCCATCCTTGAAGGGCATCCCCTTTCCGATGCATTACAGCATTTTCCCACGCTTTTCGAt                                        <  1:744467/150‑1 (MQ=255)
                                                                                                                cGGAATTCCCCCTGGAAGAGAGCCTGGCCGAACTCGTTAAACAACGCCTTAAAACACGACTGGGTGACGTGTTAAATCAGGTACGCAGCGCCATCCTTGAAGGGCATCCCCTTTCCGATGCATTACAGCATTTTCCCACGCTTTTCGAtt                                       <  2:533650/150‑1 (MQ=255)
                                                                                                                   cATTCCCCCTGGAAGAGTGCCTTCCCTTATTCGGACAACAAAGCATGAATAAACGACTGGGTGACGTGTTAAATCAGGTACGCAGCGCCATCCTTGAAGGTCATCCCCTTTCCGAGGCATTACAGCATGTTCCCACGCTTTTGGATTCGc                                    <  1:2863409/150‑1 (MQ=255)
                                                                                                                     ttACCCCTGGAAGAGAGCCTTGCCGTAATCGGTCAACAAAGCAGTAATAAACGACTGGGTGACGTGTTAAATCAGGTACGCAGCGCCATCCTTGAAGGGCATCCCCTTTCCGATGCATTACAGCATTTTCCCACGCTGTTCGATTCGctc                                  >  2:2896922/1‑150 (MQ=255)
                                                                                                                        ccccTGGAAGAGAGCCTTGCCGTAATCGGTCAACAAAGCAGTAATAAACGACTGGGTGACGTGTTAAATCAGGTACGCAGCGCCATCCTTGAAGGGCATCCCCTTTCCGATGCATTACAGCATTTTCCCACGCTTTTCGATTCGCTCTAt                               >  1:2611285/1‑150 (MQ=255)
                                                                                                                         cccTGGAAGAGAGCCTTGCCGTAATCGGTCAACAAAGCAGTAATAAACGACTGGGTGACGTGTTAAATCAGGTACGCAGCGCCATCCTTGAAGGGCATCCCCTTTCCGATGCATTACAGCATTTTCCCACGCTTTTCGATTCGCTCTATc                              >  2:944026/1‑150 (MQ=255)
                                                                                                                           cTGGAAGAGAGCCTTGCCGTAATCGGTCAACAAAGCAGTAATAAACGACTGGGTGACGTGTTAAATCAGGTACGCAGCGCCATCCTTGAAGGGCATCCCCTTTCCGATGCATTACAGCATTTTCCCACGCTTTTCGATTCGCTCTATCGt                            >  1:2831583/1‑150 (MQ=255)
                                                                                                                           cTGGAAGAGAGCCTTGCCGTAATCGGTCAACAAAGCAGTAATAAACGACTGGGTGACGTGTTAAATCAGGTACGCAGCGCCATCCTTGAAGGGCATCCCCTTTCCGATGCATTACAGCATTTTCCCACGCTTTTCGATTCGCTCTATCGt                            >  2:576272/1‑150 (MQ=255)
                                                                                                                              gAAGAGAGCCTTGCCGTAATCGGTCAACAAAGCAGTAATAAACGACTGGGTGACGTGTTAAATCAGGTACGCAGCGCCATCCTTGAAGGGCATCCCCTTTCCGATGCATTACAGCATTTTCCCACGCTTTTCGATTCGCTCTATCGTAcc                         >  1:2402457/1‑150 (MQ=255)
                                                                                                                                 gagagCCTTGCCGTAATCGGTCAACAAAGAAGTAATAAACGACTGGGTGACGTGTTAAATCAGGTACGCAGCGCCATCCTTGAAGGGCATCCCCTTTCCGATGCATTACAGCATTTTCCCACGCTTTTCGATTCGCTCTATCGTACCCTg                      <  2:258702/150‑1 (MQ=255)
                                                                                                                                 gagagCCTGGCCGTAATCGGTCAACAAAGCAGTAATAAACGACTGGGTGACGTGTTAAATCAGGTACGCAGCGCCATCCTTGAAGGGCATCCCCTTTCCGATGCATTACAGCATTTTCCCACGCTTTTCGATTCGCTCTATCGTACCCTg                      <  2:2792265/150‑1 (MQ=255)
                                                                                                                                  agagCCTTGCCGTAATCGGTCAACAAAGCTGTAATAAACGACTGGGTGACGTGTTAAATCAGGTACGCAGCGCCATCCTTGAAGGGCATCCCCTTTCCGATGCATTACAGCATTTTCCCACGCTTTTCGATTCGCTCTATCGTACCCTgg                     <  2:1536747/150‑1 (MQ=255)
                                                                                                                                    agCCTTGCCGTAATCGGTCAACAAAGCAGTAATAAACGACTGGGTGACGTGTTAAATCAGGTACGCAGCGCCATCCTTGAAGGGCATCCCCTTTCCGATGCATTACAGCATTTTCCCACGCTTTTCGATTCGCTCTATCGTACCCTGGTa                   >  1:3055419/1‑150 (MQ=255)
                                                                                                                                     gCCTTGCCGTAATCGGTCAACAAAGCAGTAATAAACGACTGGGTGACGTGTTAAATCAGGTACGCAGCGCCATCCTTGAAGGGCATCCCCTTTCCGATGCATTACAGCATTTTCCCACGCTTTTCGATTCGCTCTATCGTACCCTGGTaa                  >  1:1998492/1‑150 (MQ=255)
                                                                                                                                      ccTTGCCGTAATCGGTCAAAAAAGCAGTAATAAACGACTGGGTGACGTGATAAATCAGGTACGCAGCGCCATACTAGAAGGGCATCCACTTTCAGAAGAATAACAGCATAATACCACGCTTTTAGAATAGATCTATCGAAACATCGTaaa                 >  2:814250/1‑150 (MQ=255)
                                                                                                                                          gCCGTAATCGGTCAACAAAGCAGTAATAAACGACTGGGTGACGTGTTAAATCAGGTACGCAGCGCCATCCTTGAAGGGCATCCCCTTTCCGATGCATTACAGCATTTTCCCACGCTTTTCGATTCGCTCTATCGTACCCTGGTAAAAGCg             <  2:2601031/150‑1 (MQ=255)
                                                                                                                                           ccGTAATCGGTCAACAAAGCAGTAATAAACGACTGGGTGACGTGTTAAATCAGGTACGCAGCGCCATCCTTGAAGGGCATCCCCTTTCCGATGCATTACAGCATTTTCCCACGCTTTTCGATTCGCTCTATCGTACCCTGGTAAAAGCgg            >  1:1041008/1‑150 (MQ=255)
                                                                                                                                           ccGTAATCGGTCAACAAAGCAGTAATAAACGACTGGGTGACGTGTTAAATCAGGTACGCAGCGCCATCCTTGAAGGGCATCCCCTTTCCGATGCATTACAGCATTTTCCCACGCTTTTCGATTCGCTCTATCGTACCCTGGTAAAAGCgg            <  2:2830574/150‑1 (MQ=255)
                                                                                                                                           ccGTAATCGGAAAAAAAAGCATTAATAAACGACTGGGTGACGTGTTAAATCAGGTACGCAGCGCCATCCTTGAAGGGCATCCCCTTTCCGATGCATTACAGCATTTTCCCACGCTTTTCGATTCGCTCTATCGTACCCTGGTAAAAGCgg            <  1:1361694/150‑1 (MQ=255)
                                                                                                                                            cttAATCGGAAAACAAAGAATTAATACACGACTGGGTGACGTGTTAAATCAGGTACGCAGCGCCATCCTTGAAGGGCATCCCCTTTCCGATGCATTACAGCATTTTCCCACGCTTTTCGATTCGCTCTATCGTACCCTGGTAAAAGCggg           <  1:2917340/148‑1 (MQ=255)
                                                                                                                                            cGTAATCGGTCAACAAAGCAGTAATAAACGACTGGGTGACGTGTTAAATCAGGTACGCAGCGCCATCCTTGAAGGGCATCCCCTTTCCGATGCATTACAGCATTTTCCCACGCTTTTCGATTCGCTCTATCGTACCCTGGTAAAAGCggg           >  1:152668/1‑150 (MQ=255)
                                                                                                                                               aaTCGGTCAACAAAGCAGTAATAAACGACTGGGTGACGTGTTAAATCAGGTACGCAGCGCCATCCTTGAAGGGCATCCCCTTTCCGATGCATTACAGCATTTTCCCACGCTTTTCGATTCGCTCTATCGTACCCTGGTAAAAGCGGGCGa        <  1:1925340/150‑1 (MQ=255)
                                                                                                                                                    gTCAACAAAGCAGTAATAAACGACTGGGTGACGTGTTAAATCAGGTACGCAGCGCCATCCTTGAAGGGCATCCCCTTTCCGATGCATTACAGCATTTTCCCACGCTTTTCGATTCGCTCTATCGTACCCTGGTAAAAGCGGGCGAAAaga   >  1:15377/1‑150 (MQ=255)
                                                                                                                                                     tCAACAAAGCATTAATAAACGACTGGGTGACGTGTTAAATCAGGTACGCAGCGCCATCCTTGAAGGGCATCCCCTTTCCGATGCATTACAGCATTTTCCCACGCTTTTCGATTCGCTCTATCGTACCCTGGTAAAAGCGGGCGAAAagag  <  2:1713948/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
TTCCTGCTGGATATTCGCCCCCAAAAAAGTTCGGGAGTAAAAACACGTCGCCCGAGGATCAGCCATAGTGAACTGACGCTTTTCACCCGGCAGTTGGCAACCTTAAGCGCAGCGGCATTACCCCTGGAAGAGAGCCTTGCCGTAATCGGTCAACAAAGCAGTAATAAACGACTGGGTGACGTGTTAAATCAGGTACGCAGCGCCATCCTTGAAGGGCATCCCCTTTCCGATGCATTACAGCATTTTCCCACGCTTTTCGATTCGCTCTATCGTACCCTGGTAAAAGCGGGCGAAAAGAG  >  NC_000913/3459925‑3460223

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑