Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,781,022 T→G 16.9% intergenic (+5/‑193) lldD → / → trmL L‑lactate dehydrogenase, FMN‑linked/tRNA Leu mC34,mU34 2'‑O‑methyltransferase, SAM‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,781,0220TG16.9% 477.0 / 42.4 214intergenic (+5/‑193)lldD/trmLL‑lactate dehydrogenase, FMN‑linked/tRNA Leu mC34,mU34 2'‑O‑methyltransferase, SAM‑dependent
Reads supporting (aligned to +/- strand):  ref base T (53/122);  new base G (37/2);  total (90/124)
Fisher's exact test for biased strand distribution p-value = 2.09e-14
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 219 total aligned reads displayed.

TCTGATCGAAAAAGAGATGAAAGTGGCGATGACGCTGACTGGCGCGAAATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCA  >  NC_000913/3780873‑3781167
                                                                                                                                                     |                                                                                                                                                  
  tGATCGAAAAAGAGATGAAAGTGGCGATGACGCTGACTGGCGCGAAATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTg                                                                                                                                                  <  1:1652169/150‑1 (MQ=255)
    aTCGAAAAAGAGATGAAAGTGGCGATGACGCTGACTGGCGCGAAATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTgcg                                                                                                                                                <  2:247030/150‑1 (MQ=255)
    aTCGAAAAAGAGATGAAAGTGGCGATGACGCTGACTGGCGCGAAATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTgcg                                                                                                                                                >  1:1995648/1‑150 (MQ=255)
    aTCGAAAAAGAGATGAAAGTGGCGATGACGCTGACTGGCGCGAAATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTgcg                                                                                                                                                >  2:2793365/1‑150 (MQ=255)
    aTCGAAAAAGAGATGAAAGTGGCGATGACGCTGACTGGCGCGAAATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTgcg                                                                                                                                                >  1:1212491/1‑150 (MQ=255)
       gAAAAAGAGATGAAAGTGGCGATGACGCTGACTGGCGCGAAATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGccc                                                                                                                                             >  1:1325339/1‑150 (MQ=255)
       gAAAAAGAGAAGAAAGTGGCGATTACGCTGACTGGCGCGAAATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTGTGAGccc                                                                                                                                             >  1:2370048/1‑150 (MQ=255)
        aaaaaGAGATGAAAGTGGCGATGACGCTGACTGGCGCGAAATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGcccc                                                                                                                                            <  1:1680671/150‑1 (MQ=255)
        aaaaaGAGATGAAAGTGGCGATGACGCTGACTGGCGCGAAATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGcccc                                                                                                                                            <  1:1158442/150‑1 (MQ=255)
        aaaaaGAGATGAAAGTGGCGATGACGCTGACTGGCGCGAAATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGcccc                                                                                                                                            >  1:578296/1‑150 (MQ=255)
         aaaaGAGATGAAAGTGGCGATGACGCTGACTGGCGCGAAATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTGTGCGccccc                                                                                                                                           >  2:366843/1‑150 (MQ=255)
          aaaGAGATGAAAGTGGCGATGACGCTGACTGGCGCGAAATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGcccccg                                                                                                                                          >  1:1743997/1‑149 (MQ=255)
          aaaGAGATGAAAGTGGCGATGACGCTGACTGGCGCGAAATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTGTGCGccccca                                                                                                                                          >  1:1953667/1‑149 (MQ=255)
              agaTGAAAGTGGCGATGACGCTGACTGGCGCGAAATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCAcc                                                                                                                                      <  2:1219542/150‑1 (MQ=255)
                aTGAAAGTGGCGATGACGCTGTCTGGCGCGAAATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTGTGCGCCCCCCCAccca                                                                                                                                    >  1:1982814/1‑149 (MQ=255)
                aTGAAAGTGGCGATGACGCTGACTGGCGCGAAATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTGTGCgcccccgccccca                                                                                                                                    >  1:1413475/1‑149 (MQ=255)
                aTGAAAGTGGCGATGACGCTGACTGGCGCGAAATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTGTGCGCCCCCACAcccg                                                                                                                                    >  2:2017500/1‑149 (MQ=255)
                aTGAAAGTGGCGATGACGCTGACTGGCGCGAAATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCAGGGCGAAGGGGAATGCGGCATGGTCGGGTGCGgccccccgcgcg                                                                                                                                    >  1:2675326/1‑143 (MQ=255)
                  gAAAGTGGCGATGACGCTGACTGGCGCGAAATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCACACCCCaa                                                                                                                                  >  2:124719/1‑150 (MQ=255)
                  gAAAGTGGCGATGACGCTGACTGGCGCGAAATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTGTGCGCCCCCCCACCCaaa                                                                                                                                  >  1:2944873/1‑150 (MQ=255)
                  gAAAGTGGCGATGACGCTGACTGGCGCGAAATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTGTGCGCCCCCACCCCCCaa                                                                                                                                  >  1:2122172/1‑150 (MQ=255)
                  gAAAGTGGCGATGACGCTGACTGGCGCGAAATCGATCAGCGAAATTACGCAAGAGTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTaa                                                                                                                                  <  2:2243287/150‑1 (MQ=255)
                    aaGTGGCGATGACGCTGACTGGCGCGAAATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCGATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAAcc                                                                                                                                <  1:1946417/150‑1 (MQ=255)
                    aaGTGGCGATGACGCTGACTGGCGCGAAATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTGTTCGCCCCCCCACCCaaaac                                                                                                                                >  1:1427848/1‑148 (MQ=255)
                       tGGCGATGACGCTGACTGGCGCGAAATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTGTGCGCCCCCACACCCCaaaacac                                                                                                                             >  1:1059546/1‑145 (MQ=255)
                        ggCGATGACGCTGACTGGCGCGAAATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCctct                                                                                                                            <  2:2942136/150‑1 (MQ=255)
                        ggCGATGACGCTGACTGGCGCGAAATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCctct                                                                                                                            <  1:2475637/150‑1 (MQ=255)
                        ggCGATGACGCTGACTGGCGCGAAATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTGTGCGCCCCCACAACCAAAaccaca                                                                                                                            >  1:3105933/1‑147 (MQ=255)
                         gCGATGACGCTGACTGGCGCGAAATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCtctc                                                                                                                           <  2:1567864/150‑1 (MQ=255)
                           gATGACGCTGACTGGCGCGAAATCGATCAGCGAAATTACGCAAGAGTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTGGGCGCCCCCCCCCCCGAACCCACcccc                                                                                                                         >  2:2260968/1‑150 (MQ=255)
                              gACGCTGACTGGCGCGAAATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCa                                                                                                                      <  1:2559846/150‑1 (MQ=255)
                              gACGCTGACTGGCGCGAAATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTGTGCGCCCCCACACCCAAACCCACTACcaca                                                                                                                      >  2:2040165/1‑150 (MQ=255)
                                cGCTGACTGGCGCGAAATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAgg                                                                                                                    <  2:368957/150‑1 (MQ=255)
                                  cTGACTGGCGCGAAATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCAAACCCAATCCCACAGGga                                                                                                                  >  1:2049403/1‑150 (MQ=255)
                                   tGACTGGCGCGAAATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAAcc                                                                                                                                >  1:1233414/1‑135 (MQ=255)
                                     aCTGGCGCGAAATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGagag                                                                                                               <  1:2582039/150‑1 (MQ=255)
                                      cTGGCGCGAAATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAgg                                                                                                              <  2:2099344/150‑1 (MQ=255)
                                         gcgcGAAATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTGTGCGCCCCCCCACCCAAAACCACACCCCCACGggggggggc                                                                                                           >  2:273908/1‑149 (MQ=255)
                                                ctCGATCAGCGAAATTACGCAAGATTCGCTGGTGCTGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAATGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCg                                                                                                    <  1:1143543/149‑1 (MQ=255)
                                                   gATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCACACCCCAACCCCCACCCACAGGGCGAGGGGCCCCGTCGgcg                                                                                                 >  1:2211288/1‑150 (MQ=255)
                                                   gATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCACACCCAAACCCACAACCCCCGGGAGAGGGGGCCCGTCgggg                                                                                                 >  1:2639284/1‑148 (MQ=255)
                                                    aTCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGgcgc                                                                                                <  2:177938/150‑1 (MQ=255)
                                                    aTCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCACACCCCAAACCACAACCACACGGGGAGGGGGCCCGTAGgcgc                                                                                                >  1:557336/1‑150 (MQ=255)
                                                    aTCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTGTGCGCCCCCCCACCCCAACCCACTCCCGCAGGGAGGGGGGCCCCGTCGgcgc                                                                                                >  1:88207/1‑150 (MQ=255)
                                                     tCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTTCGCCCCCCCCCCCAAAACCCCAACCACACGGGGACGGGCCCCGTCGgcgcc                                                                                               >  2:1884792/1‑149 (MQ=255)
                                                      cAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCtg                                                                                              <  1:2141910/150‑1 (MQ=255)
                                                      cAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCtg                                                                                              <  1:1785838/150‑1 (MQ=255)
                                                       aGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCGCACCCAAACCCACTCCCACAGGGAGAGGGGACCGTTCGGCGCtgt                                                                                             >  1:2083015/1‑150 (MQ=255)
                                                       aGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTGTGCGCCCCCCCCCCCCAAACCACACCCACACGGCGAGGGGGCCCGTCGGCGCggt                                                                                             >  2:1951965/1‑150 (MQ=255)
                                                          gAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCtgtatg                                                                                          <  2:1969857/150‑1 (MQ=255)
                                                          gAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCtgtatg                                                                                          <  2:762599/150‑1 (MQ=255)
                                                          gAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCtgtatg                                                                                          <  1:2675409/150‑1 (MQ=255)
                                                           aaaTTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTTCGCCCCCGCACCCGAACCCTCTCCCCCAGGGGGGGGGGGCCGGTAGGCGCgggatgt                                                                                         >  2:1727200/1‑150 (MQ=255)
                                                                aCGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTcc                                                                                    <  2:69830/150‑1 (MQ=255)
                                                                 cGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCACACCCAAACCCAATCCCACAGGGGGAGGGGACCCTTAGGCGCGGTTTGTAATccc                                                                                   >  1:2331705/1‑150 (MQ=255)
                                                                  gCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTGTGCGCCCCCCCCCCCCAAACCACACCCCCAGGGGGGGGGGGCCCGTGGGCGCGGTATTTAcggccc                                                                                  >  2:2876894/1‑145 (MQ=255)
                                                                    aaGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCa                                                                                <  1:1033098/150‑1 (MQ=255)
                                                                    aaGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCa                                                                                <  1:2649675/150‑1 (MQ=255)
                                                                    aaGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCACACCCAAAACCAAACCCACAGGGCGAGGGGACCCGTCGGCGCTTGATGTACACCCTCa                                                                                >  1:463676/1‑150 (MQ=255)
                                                                     aGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCAAACACTCTCCCACAGGGCGAGGGGACCCATAGGCGCTGTTTGTAATCCCacac                                                                               >  2:456766/1‑150 (MQ=255)
                                                                      gATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCAct                                                                             <  2:290577/150‑1 (MQ=255)
                                                                       aTTCGCTGGGGCAGGGGCGGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCActc                                                                            <  1:540481/150‑1 (MQ=255)
                                                                        ttCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCActct                                                                           <  1:2606771/150‑1 (MQ=255)
                                                                         tCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTg                                                                          <  2:2795970/150‑1 (MQ=255)
                                                                         tCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTg                                                                          <  2:2634553/150‑1 (MQ=255)
                                                                           gCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGaa                                                                        <  2:1352213/150‑1 (MQ=255)
                                                                            cTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGaaa                                                                       <  1:2490853/150‑1 (MQ=255)
                                                                             tGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCGCACCCAAACCCACACCCACAGGGGGAGGGGCCCGGTCGGCGCTGTATGTAATGCCGCACGCAGAAAc                                                                      >  1:3013215/1‑150 (MQ=255)
                                                                                               aaGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCtttttt                                                    <  2:1649567/150‑1 (MQ=255)
                                                                                                 gagTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTTCGCCCCCGCACCCAAACCCACTCCCGCAGGGCGAGGGGCCCCTGCGGCGGCGGGGGTACTCCCTCACCCGGCCACGGCCCCGCACGCatgttttg                                                  >  2:2174687/1‑149 (MQ=255)
                                                                                                 gagTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTtc                                                  <  1:404701/150‑1 (MQ=255)
                                                                                                 gagTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTtc                                                  <  1:3042592/150‑1 (MQ=255)
                                                                                                  agTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACTGCACTCTTTTTTtct                                                 <  2:1441301/150‑1 (MQ=255)
                                                                                                  agTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCATAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTtct                                                 <  1:1792765/150‑1 (MQ=255)
                                                                                                    ttGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTGTGCGCCCCCGCCCCCTAAACCACTCCCGCAGGGGGAGGGGGCCGTTAGGCGCGGTTTTTACTCCCGCACACTGGAACGGCACCGCCCAGtttttttttgc                                               >  1:2043521/1‑146 (MQ=255)
                                                                                                         tGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGc                                          <  1:994577/150‑1 (MQ=255)
                                                                                                          gCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCGCACCCGAACCCACTCCCTCACGGCGTGGGGGCCGGTCGGCGCGGGATGTAATCCCGCACTCTGTAACCACACCGCCCGCTTTTTTCAACCTCGccc                                        >  2:1559811/1‑150 (MQ=255)
                                                                                                          gCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTGTTGGCCCCCAAACCCGAACACAAAACCAGCGGGGGGTGGGGCCGTTGGGCGCTGGATTTTAACCCTAACGGGTGAAACACAACGCCCTCTTTTTTTCTCCCACGcc                                         >  1:1478676/1‑150 (MQ=255)
                                                                                                                 tGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCgg                                  <  1:1891246/150‑1 (MQ=255)
                                                                                                                 tGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCgg                                  <  1:1891318/150‑1 (MQ=255)
                                                                                                                  ggCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCCGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCggg                                 <  2:1325339/150‑1 (MQ=255)
                                                                                                                  ggCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTGGCGCCCCCTCACCCTAACCCTCTCCCTCCGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCggg                                 <  1:2410655/150‑1 (MQ=255)
                                                                                                                     tCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGgag                              <  2:2957337/150‑1 (MQ=255)
                                                                                                                      cccATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGgaga                             <  2:3105933/150‑1 (MQ=255)
                                                                                                                          tGGCGAAAGGGAATGCGGCATAGTCGTGTGCGCCCCCTCACCCTACCCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGc                         <  2:2359024/150‑1 (MQ=255)
                                                                                                                           ggCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCGCACCAAAACCCACTACCACAGGGGGAGGGGGCCGTTCGGCGCGGTTATTACTCCCGCACTCTGAAACGAAACCCCACACATTTTTTATACCGCGCCCCACCCGGGCGGGGGcc                        >  1:2524298/1‑150 (MQ=255)
                                                                                                                                  gggAATGCGGCATAGTCGTTTGCGCCCCCTCCCCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGa                 <  2:840403/150‑1 (MQ=255)
                                                                                                                                   ggATTGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGTGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGTGGGCCGGGGTGAg                <  1:2793365/150‑1 (MQ=255)
                                                                                                                                   ggAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCTGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTATTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAg                <  1:757738/150‑1 (MQ=255)
                                                                                                                                   ggAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCCGGGTGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAg                <  1:1316815/150‑1 (MQ=255)
                                                                                                                                   ggAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAg                <  2:1797978/150‑1 (MQ=255)
                                                                                                                                   ggAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAg                <  1:742619/150‑1 (MQ=255)
                                                                                                                                    gactGCGGCATCGTCGTTGGCGCCCCCTCCCCCTACCCCTCTCCCGCAGGGAGAGGGGACCGGTGGGCGCTGATTGGCCTCCCCCCCTCTGTCCCGACACCGCACTCTTTTTGCCTCGCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAgg               <  1:2361290/147‑1 (MQ=255)
                                                                                                                                    gAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTACCCCTCTCCCTCAGGGAGAGGGGACCGGGCGGCGCTGGATGGACTCCCTCACTCTGACACGACACCGCACTCTTTTGTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAgg               <  2:753183/150‑1 (MQ=255)
                                                                                                                                     aaTGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCGTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAggg              <  2:1354785/150‑1 (MQ=255)
                                                                                                                                       tGCGGCATAGTCGTTTGCGCCCCCTCACCCTACCCCTCGCCCTCCGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGa            <  2:944540/150‑1 (MQ=255)
                                                                                                                                         cGGCATAGTCGTGTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGaaa          <  2:265857/150‑1 (MQ=255)
                                                                                                                                          ggCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCTGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGaaaa         <  1:2535323/150‑1 (MQ=255)
                                                                                                                                            cATAGTCGTTTTCGCCCCCACACCCAAAACCAAAACCACAGGGGGAGGGGCCCCGTAGGCGCAGTATTTAAACCCGCACACATAAACGACACCCCACACATTTTTTCTCCCACCCCCCAACCGGGAGAGGGCCGGGGTGAGGGGGAAAgg       >  2:507779/1‑150 (MQ=255)
                                                                                                                                                 tCGTTTGCGCCCCCACACCCAAAACCACTCCCTCAGGGAGAGGGGACCCTTCGGCGCTGTATGTACGCCCTCACTCAGAAACTACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGGGAGGGGAAAAGGCCGCa  >  2:259507/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                  
TCTGATCGAAAAAGAGATGAAAGTGGCGATGACGCTGACTGGCGCGAAATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCA  >  NC_000913/3780873‑3781167

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: