Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 71,999 T→G 11.7% L217V (TTG→GTG)  yabI → DedA family inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_00091371,9990TG11.7% 2030.8 / 73.1 773L217V (TTG→GTG) yabIDedA family inner membrane protein
Reads supporting (aligned to +/- strand):  ref base T (249/414);  new base G (107/3);  total (356/417)
Fisher's exact test for biased strand distribution p-value = 2.04e-36
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 809 total aligned reads displayed.

ATATTCCTGCCGGAATGCAGAGCGGTGAGTTTAAATGGTTGCTGCTGGCAACAGCGGTGTTTTTGTGGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCGACTGACCGTTTGAGTCATTATTTGTCCCGCGGTCGTTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCACCCGTTGATGCCGGTGTATATCGATATTTTGCGTAAAGTGGTTGGGGTTTAGGAGATAGTCTTGTGCGGGTTGCCTGATGCGACG  >  NC_000913/71850‑72148
                                                                                                                                                     |                                                                                                                                                     
atatTCCTGCCGGAATGCAGAGCGGTGAGTTTAAATGGTTGCTGCTGGCAACAGCGGTGTTTTTGTGGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCGACTGACCGTTTGAGTCATTATTTGTCCCGCGGTCGtt                                                                                                                                                       >  2:3541891/1‑150 (MQ=255)
 tatTCCTGCCGGAATGCAGAGCGGTGAGTTTAAATGGTTGCTGCTGGCAACAGCGGTGTTTTTGTGGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCGACTGACCGTTTGAGTCATTATTTGTCCCGCGGTCGttt                                                                                                                                                      <  1:5168171/150‑1 (MQ=255)
 tatTCCTGCCGGAATGCAGAGCGGTGAGTTTAAATGGTTGCTGCTGGCAACAGCGGTGTTTTTGTGGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCGACTGACCGTTTGAGTCATTATTTGTCCCGCGGTCGttt                                                                                                                                                      <  1:6850223/150‑1 (MQ=255)
  atTCCTGCCGGAATGCAGAGCGGTGAGGTTAAATGGTTGCTGCTGGCAACAGCGGTGTTTGTGTGGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCGACTGACCGTTTGAGTCATTATTTGTCCCGCGGTCGtgtg                                                                                                                                                     >  1:2139824/1‑150 (MQ=255)
       tGCCGGAATGCAGAGCGGTGAGTTTAAATGGTTGCTGCTGGCAACAGCGGTGTTTTTGTGGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCGACTGACCGTTTGAGTCATTATTTGTCCCGCGGTCGTTTGTtgtg                                                                                                                                                <  2:7137322/150‑1 (MQ=255)
       tGCCGGAATGCAGAGCGGTGAGTTTAAATGGTTGCTGCTGGCAACAGCGGTGTTTTTGTGGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCGACTGACCGTTTGAGTCATTATTTGTCCCGCGGTCGTTTGTtgtg                                                                                                                                                <  1:6126239/150‑1 (MQ=255)
        gCCGGAATGCAGAGCGGTGAGTTTAAATGGTTGCTGCTGGCAACAGCGGTGTTTTTGTGGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCGACTGACCGTTTGAGTCATTATTTGTCCCGCGGTCGTTTGTTGTgg                                                                                                                                               <  2:11278978/150‑1 (MQ=255)
          cGGAATGCAGAGCGGTGAGTTTAAATGGTTGCTGCTGGCAACAGCGGTGTTTTTGTGGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCGACTGACCGTTTGAGTCATTATTTGTCCCGCGGTCGTTTGTTGTGGCt                                                                                                                                             >  1:4807267/1‑150 (MQ=255)
           ggAATGCAGAGCGGTGAGTTTAAATGGTTGCTGCTGGCAACAGCGGTGTTTTTGTGGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCGACTGACCGTTTGAGTCATTATTTGTCCCGCGGTCGTTTGTTGTGGCTg                                                                                                                                            >  2:1026882/1‑150 (MQ=255)
            gAATGCAGAGCGGTGAGTTTAAATGGTTGCTGCTGGCAACAGCGGTGTTTTTGTGGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCGACTGACCGTTTGAGTCATTATTTGTCCCGCGGTCGTTTGTTGTGGCTGa                                                                                                                                           <  1:10816614/150‑1 (MQ=255)
               tGCAGAGCGGTGAGTTTAAATGGTTGCTGCTGGCAACAGCGGTGTTTTTGTGGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCGACTGACCGTTTGAGTCATTATTTGTCCCGCGGTCGTTTGTTGTGGCTGACGc                                                                                                                                        >  2:2472270/1‑150 (MQ=255)
               tGCAGAGCGGTGAGTTTAAATGGTTGCTGCTGGCAACAGCGGTGTTTTTGTGGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCGACTGACCGTTTGAGTCATTATTTGTCCCGCGGTCGTTTGTTGTGGCTGACGc                                                                                                                                        <  1:7003810/150‑1 (MQ=255)
               tGCAGAGCGGTGAGTTTAAATGGTTGCTGCTGGCAACAGCGGTGTTTTTGTGGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCGACTGACCGTTTGAGTCATTATTTGTCCCGCGGTCGTGTGTTGTGGCTGACGc                                                                                                                                        >  2:3273788/1‑150 (MQ=255)
                 cAGAGCGGTGAGTTTAAATGGTTGCTGCTGGCAACAGCGGTGTTTTTGTGGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCGACTGACCGTTTGAGTCATTATTTGTCCCGCGGTCGTTTGTTGTGGCTGACGCCg                                                                                                                                      >  2:4741820/1‑150 (MQ=255)
                   gagCGGTGAGTTTAAATGGTTGCTGCTGGCAACAGCGGTGTTTTTGTGGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCGACTGACCGTTTGAGTCATTATTTGTCCCGCGGTCGTTTGTTGTGGCTGACGCCGtt                                                                                                                                    >  2:9136670/1‑150 (MQ=255)
                    agCGGTGAGTTTAAATGGTTGCTGCTGGCAACAGCGGTGTTTTTGTGGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCGACTGACCGTTTGAGTCATTATTTGTCCCGCGGTCGTGTGTTGTGGCTGACCCCGTTg                                                                                                                                   >  1:4381825/1‑150 (MQ=255)
                      cGGTGAGTTTAAATGGTTGCTGCTGGCAACAGCGGTGTTTTTGTGGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCGACTGACCGTTTGAGTCATTATTTGTCCCGCGGTCGTTTGTTGTGGCTGACGCCGTTGAt                                                                                                                                 <  1:8429662/150‑1 (MQ=255)
                           aGTTTAAATGGTTGCTGCTGGCAACAGCGGTGTTTTTGTGGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCGACTGACCGTTTGAGTCATTATTTGTCCCGCGGTCGTTTGTTGTGGCTGACGCCGTTGATTTCTg                                                                                                                            <  2:4551425/150‑1 (MQ=255)
                             tttAAATGGTTGCTGCTGGCAACAGCGGTGTTTTTGTGGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCGACTGACCGTTTGAGTCATTATTTGTCCCGCGGTCGTTTGTTGTGGCTGACGCCGTTGATTTCTGcc                                                                                                                          >  2:8835538/1‑150 (MQ=255)
                             tttAAATGGTTGCTGCTGGCAACAGCGGTGTTTTTGTGGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCGACTGACCGTTTGAGTCATTATTTGTCCCGCGGTCGTTTGTTGTGGCTGACGCCGTTGATTTCTGcc                                                                                                                          >  2:9825709/1‑150 (MQ=255)
                             tttAAATGGTTGCTGCTGGCAACAGCGGTGTTTTTGTGGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCGACTGACCGTTTGAGTCATTATTTGTCCCGCGGCCGTTTGTTGTGGCTGACGCCGTTGATTTCTGcc                                                                                                                          >  2:1885201/1‑150 (MQ=255)
                             tttAAATGGTTGCTGCTGGCAACAGCGGTGTTTTTGTGGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCGACTGACCGTTTGAGTCAGTATTTGTCCCGCGGTCGTGTGTTGTGGCTGACGCCGTTGATTTCTGcc                                                                                                                          >  2:10662622/1‑150 (MQ=255)
                             tttAAATGGTTGCTGCTGGCAACAGCGGTGTTTTTGTGGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCGACTGACCGTGTGAGTCATTATTTGTCCCGCGGTCGTGTGTTGTGGCTGACGCCGTTGATTTATGcc                                                                                                                          >  2:8558895/1‑150 (MQ=255)
                               tAAATGGTTGCTGCTGGCAACAGCGGTGTTTTTGTGGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCGACTGACCGTTTGAGTCAGTATTTGTCCCGCGGTCGTGTGTTGTGGCTGACGCCGGTGATTTCTGccct                                                                                                                        >  1:7989103/1‑148 (MQ=255)
                                aaaTGGTTGCTGCTGGCAACAGCGGTGTTTTTGTGGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCGACTGACCGTTTGAGTCATTATTTGTCCCGCGGTCGTTTGTTGTGGCTGACGCCGTTGATTTCTGCCATc                                                                                                                       <  1:10119318/150‑1 (MQ=255)
                                aaaTGGTTGCTGCTGGCAACAGCGGTGTTTTTGTGGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCGACTGACCGTTTGAGTCATTATTTGTCCCGCGGTCGTTTGTTGTGGCTGACGCCGTTGATTTCTGCCATc                                                                                                                       >  1:6364106/1‑150 (MQ=255)
                                aaaTGGTTGCTGCTGGCAACAGCGGTGTTTTTGTGGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCGACTGACCGTTTGAGTCATTATTTGTCCCGCGGTCGTGTGTTGTGGCTGTCGCCGTTGATTTCTTCCATc                                                                                                                       >  1:2964203/1‑150 (MQ=255)
                                      ttGCTGCTGGCAACAGCGGTGTTTTTGTGGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCGACTGACCGTTTGAGTCATTATTTGTCCCGCGGTCGTTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCgtg                                                                                                                 <  1:3671145/150‑1 (MQ=255)
                                      ttGCTGCTGGCAACAGCGGTGTTTTTGTGGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCGACTGACCGTTTGAGTCATTATGTGTCCCGCGGTCGTGTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCgtg                                                                                                                 >  2:8347353/1‑150 (MQ=255)
                                         ctgctgGCAACAGCGGTGTTTTTGTGGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCGACTGACCGTTTGAGTCATTATTTGTCCCGCGGTCGTTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCgtggtg                                                                                                              <  1:6563855/150‑1 (MQ=255)
                                         ctgctgGCAACAGCGGTGTTTGTGGGGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCGACTGACCGTTTGAGTCATTATTTGTCCCGCGGTCGTTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCgtggtg                                                                                                              <  2:3669723/150‑1 (MQ=255)
                                          tgctgGCAACAGCGGTGTTTTTGTGGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCGACTGACCGTTTGAGTCATTATTTGTCCCGCGGTCGTTTGTTGTGGCTGACGCCGGTGATTTATGCCATCGGCGtggtgg                                                                                                             >  1:7698240/1‑150 (MQ=255)
                                          tgctgGCAACAGCGGTGTTTTTGTGGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCGACTGACCGTTTGAGTCAGTATTTGTCCCGCGGTCGTGTGTTGTGGCTGACGCCGTTGGTTTATGCCATCGGCGtggtgg                                                                                                             >  1:9832034/1‑150 (MQ=255)
                                            ctgGCAACAGCGGTGTTTTTGTGGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCGACTGACCGTTTGAGTCATTATTTGTCCCGCGGTCGTTTGTTGTGGCTGTCGCCGGTGATTTATGCCATCGGCGTGGTGgcg                                                                                                           >  1:9521192/1‑150 (MQ=255)
                                            ctgGCAACAGCGGTGTTTTTGTGGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCGACTGACCGTTTGAGTCATTATTTGTCCCGCGGTCGTGTGTTGTTGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGgcg                                                                                                           >  1:11782360/1‑150 (MQ=255)
                                            ctgGCAACAGCGGTGTTTTTGTGGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCGACTGACCGTTTGAGTCATTATTTGTCCCGCGGTCGGTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGgcg                                                                                                           >  1:7148977/1‑150 (MQ=255)
                                               gCAACAGCGGTGTTTTTGTGGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCGACTGACCGTTTGAGTCATTATTTGTCCCGCGGTCGTTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCtg                                                                                                        >  2:1708160/1‑150 (MQ=255)
                                                cAACAGCGGTGTTTTTGTGGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCGACTGACCGTTTGAGTCATTATTTGTCCCGCGGTCGTTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCtgg                                                                                                       <  1:11961427/150‑1 (MQ=255)
                                                  aCAGCGGTGTTTTTGTGGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCGACTGACCGTTTGAGTCATTATTTGTCCCGCGGTCGTTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCtggtg                                                                                                     <  1:381009/150‑1 (MQ=255)
                                                   cAGCGGTGTTTTTGTGGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCGACTGACCGTTTGAGTCATTATTTGTCCCGCGGTCGTTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCtggtgg                                                                                                    <  1:5952494/150‑1 (MQ=255)
                                                    aGCGGTGTTTTTGTGGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCGACTGACCGTTTGAGTCATTATTTGTCCCGCGGTCGTTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCTggtggt                                                                                                   <  2:2960204/150‑1 (MQ=255)
                                                    aGCGGTGTTTTTGTGGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCGACTGACCGTTTGAGTCATTATTTGTCCCGCGGTCGTTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCTggtggt                                                                                                   <  1:1914517/150‑1 (MQ=255)
                                                    aGCGGTGTTTTGGTGGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCGACTGACCGTTTGAGTCATTATTTGTCCCGCGGTCGTTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCTggtggt                                                                                                   <  2:11511806/150‑1 (MQ=255)
                                                     gCGGTGTTTTTGTGGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCGACTGACCGTTTGAGTCATTATTTGTCCCGCGGTCGTTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCTGgtggtg                                                                                                  <  1:2241118/150‑1 (MQ=255)
                                                      cGGTGTTTTTGTGGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCGACTGACCGTTTGAGTCATTATTTGTCCCGCGGTCGTTTGTTGTGGCTGACGCCGATGATTTCTGCCAACGGCGTGGTGGCGCTGGTGgtgt                                                                                                 >  2:9643151/1‑150 (MQ=255)
                                                       ggTGTTTTTGTGGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCGACTGACCGTTTGAGTCATTATTTGTCCCGCGGTCGTTTGTTGTGGCTGTAGCCGTTGATTTCTGCCCTCCGCGTGGTGGCGCTGGTGGTGtt                                                                                                >  1:9271755/1‑150 (MQ=255)
                                                       ggTGTTTTTGTGGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCGACTGACCGTTTGAGTCATTATTTGTCCCGCGGTCGTGTGTTGTGGCGGTCGCCGTTGATTTCTGCCAACGGCGTGGTGGCGCTGGTGGTGtt                                                                                                >  1:2684030/1‑150 (MQ=255)
                                                            ttttGTGGGTGGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGGAAAGCGACTGACCGTTTGAGTCATTATTTGTCCCGCGGTCGTTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTc                                                                                           <  1:113689/150‑1 (MQ=255)
                                                             tttGTGGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCGACTGACCGTTTGAGTCATTATTTGTCCCGCGGTCGTTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTCg                                                                                          <  2:6447898/150‑1 (MQ=255)
                                                               tgtgGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCGACTGACCGTGTGAGTCATTATTTGTCCCGCGGTCGTTTGTTGTGGCTGACGCCGTTGATTTATGCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTCGcc                                                                                        >  2:1543106/1‑150 (MQ=255)
                                                                 tgGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCGACTGACCGTTTGAGTCATTATTTGTCCCGCGGTCGTTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCAc                                                                                      <  2:6964379/150‑1 (MQ=255)
                                                                 tgGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCGACTGACCGTTTGAGTCATTATTTGTCCCGCGGTCGTTTGTTGTGGCTGACGCCGGTGATTTCTGCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTAGCCAc                                                                                      >  1:2553908/1‑150 (MQ=255)
                                                                 tgGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCGACTGACCGTTTGAGTCATTATTTGTCCCGCGGTCGTGTGTTGTGGCTGACGCCGGTGATTTCTGCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCAc                                                                                      >  1:12156312/1‑150 (MQ=255)
                                                                   ggTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCGACTGACCGTTTGAGTCATTATTTGTCCCGCGGTCGTTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCAccc                                                                                    <  2:2156203/150‑1 (MQ=255)
                                                                     ttGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCGACTGACCGTTTGAGTCATTATTTGTCCCGCGGTCGTTTGTTGTGGCTGACGCCGTTGATTTCTGCCATAGGCGTGGTGGCGCTGGTGGGGTTAATTAGCCACCCGt                                                                                  >  1:7815506/1‑150 (MQ=255)
                                                                     ttGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCAACTGACCGTTTGAGTCATTATTTGTCCCGCGGTCGGTTGTTGTGGCTGACCCCCGTGAATTCTGCCATCGGCCTGGTGGCGCAGGGGGTGTTAATACGCCAcccct                                                                                  >  2:6232443/1‑148 (MQ=255)
                                                                      tggtggCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCGACTGACCGTTTGAGTCATTATTTGTCCCGCGGTCGTTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCACCCGtt                                                                                 <  2:7651437/150‑1 (MQ=255)
                                                                      tggtggCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCGACTGACCGTTTGAGTCATTATTTGTCCCGCGGTCGTTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCACCCGtt                                                                                 <  2:5236520/150‑1 (MQ=255)
                                                                       ggtggCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCGACTGACCGTTTGAGTCATTATTTGTCCCGCGGTCGTTTGTTGTGGCTGACGCCGTTGATTTCTGCCAACGGCGTGGTGGCGCAGGTGGTGTTTATTCGCCAccccatg                                                                                >  1:11592095/1‑146 (MQ=255)
                                                                        gtggCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCGACTGACCGTTTGAGTCATTATTTGTCCCGCGGTCGTGTGTTGTGGCTGACGCCGGTTTTTTATGCCAACGGCGTGGGGGCGCGGGTGGTGGTAATTAGCCACCCGGTGa                                                                               >  1:5732998/1‑150 (MQ=255)
                                                                         tggctggcTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCGACTGACCGTTTGAGTCATTATTTGTCCCGCGGTCGTTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCACCCGTTGAt                                                                              <  2:8653977/150‑1 (MQ=255)
                                                                         tggctggcTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCGACTGACCGTTTGAGTCATTATTTGTCCCGCGGTCGTTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCACCCGTTGAt                                                                              <  1:7412727/150‑1 (MQ=255)
                                                                           gctggctgTGCTGGCGGTTATGGCGCAGCGGTAAAGCGACTGACCGTTTGAGTCATTATTTGTCCCGCGGTCGTTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCACCCGTTGATGc                                                                            >  2:11727672/1‑150 (MQ=255)
                                                                               gctgTGCTGGCGGTTATGGCGCAGCGGTAAAGCGACTGACCGTTTGAGTCATTATTTGTCCCGCGGTCGTGTGTTGTGGCTGAAGCCGGTGGTTTATGCCAACCGCGTGGTGGCGCTGGTGGGGTTTATTCTCCCCCCCGTGATTCCggg                                                                        >  1:10401126/1‑149 (MQ=255)
                                                                                ctgTGCTGGCGGTTATGGCGCAGCGGTAAAGCGACTGACCGTTTGAGTCATTATTTGTCCCGCGGTCGTTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCACCCGTTGATGCCGgtg                                                                       <  2:5517729/150‑1 (MQ=255)
                                                                                ctgTGCTGGCGGTTATGGCGCAGCGGTAAAGCGACTGACCGTTTGAGTCATTATTTGTCCCGCGGTCGTTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCACCCGTTGATGCCGgtg                                                                       <  1:4249727/150‑1 (MQ=255)
                                                                                ctgTGCTGGCGGTTATGGCGCAGCGGTAAAGCGACTGACCGTTTGAGTCATTATTTGTCCCGCGGTCGTTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCACCCCGTGATGCCGgtg                                                                       >  2:1191350/1‑150 (MQ=255)
                                                                                 tgtgCTGGCGGTTATGGCGCAGCGGTAAAGCGACTGACCGTTTGAGTCATTATTTGTCCCGCGGTCGTTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCACCCGTTGATGCCGgtgt                                                                      <  1:8835175/150‑1 (MQ=255)
                                                                                 tgtgCTGGCGGTTATGGCGCAGCGGTAAAGCGACTGACCGTTTGAGTCATTATTTGTCCCGCGGTCGTTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCACCCGTTGATGCCGgtgt                                                                      <  1:4584125/150‑1 (MQ=255)
                                                                                    gCTGGCGGTTATGGCGCAGCGGTAAAGCGACTGACCGTTTGAGTCATTATTTGTCCCGCGGTCGTTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCACCCGTTGATGCCGGTGtata                                                                   <  2:2336984/150‑1 (MQ=255)
                                                                                     cTGGCGGTTATGGCGCAGCGGTAAAGCGACTGACCGTTTGAGTCATTATTTGTCCCGCGGTCGTTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCACCCGTTGATGCCGGTGTatat                                                                  <  2:4150125/150‑1 (MQ=255)
                                                                                        gCGGTTATGGCGCAGCGGTAAAGCGACTGACCGTTTGAGTCATTATTTGTCCCGCGGTCGTTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCACCCGTTGATGCCGGTGTATATCGa                                                               <  2:575749/150‑1 (MQ=255)
                                                                                        gCGGTTATGGCGCAGCGGTAAAGCGACTGACCGTTTGAGTCATTATTTGTCCCGCGGTCGTTTGTTGTGGCTGACGCCGTTGATTTATTCCAACGGCGTGGTGGCGCTGGTGGGGTTAATTAGCCCCCCCGTGATGCCGGTGTATATAGa                                                               >  1:11120017/1‑150 (MQ=255)
                                                                                              aTGGCGCAGCGGTAAAGCGACTGACCGTTTGAGTCATTATTTGTCCCGCGGTCGTTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCACCCGTTGATGCCGGTGTATATCGATAtttt                                                         <  2:10969639/150‑1 (MQ=255)
                                                                                                          tAAAGCGACTGACCGTTTGAGTCATTATTTGTCCCGCGGTCGTTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCACCCGTTGATGCCGGTGTATATCGATATTTTGCGTAAAGTGGt                                             <  1:9085869/150‑1 (MQ=255)
                                                                                                           aaaGCGACTGACCGTTTGAGTCATTATTTGTCCCGCGGTCGTTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCACCCGTTGATGCCGGTGTATATCGATATTTTGCGTAAAGTGGtt                                            <  1:10923343/150‑1 (MQ=255)
                                                                                                           aaaGCGACTGACCGTTTGAGTCATTATTTGTCCCGCGGTCGTTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCACCCGTTGATGCCGGTGTATATCGATATTTTGCGTAAAGTGGtt                                            <  1:8255586/150‑1 (MQ=255)
                                                                                                           aaaGCGACTGACCGTTTGAGTCATTATTTGTCCCGCGGTCGTTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCACCCGTTGATGCCGGTGTATATCGATATTTTGCGTAAAGTGGtt                                            <  2:314034/150‑1 (MQ=255)
                                                                                                              gCGACTGACCGTTTGAGTCATTATTTGTCCCGCGGTCGTTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCACCCGTTGATGCCGGTGTATATCGATATTTTGCGTAAAGTGGTTggg                                         <  2:101569/150‑1 (MQ=255)
                                                                                                                gACTGACCGTTTGAGTCATTATTTGTCCCGCGGTCGTTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCACCCGTTGATGCCGGTGTATATCGATATTTTGCGTAAAGTGGTTGGGGt                                       <  2:6791619/150‑1 (MQ=255)
                                                                                                                     aCCGTTTGAGTCATTATTTGTCCCGCGGTCGTTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCACCCGTTGATGCCGGTGTATATCGATATTTTGCGTAAAGTGGTTGGGGTTTAgg                                  <  1:10685246/150‑1 (MQ=255)
                                                                                                                     aCCGTTTGAGTCATTATTTGTCCCGCGGTCGTTTGTTGTGGCTGACGCCGTTGATTTCTGCCAAAGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCACCCCGTGATGCCGGTGTATATAGATATTTTGCGTAAAGTGGGTGGGGTTTatg                                  >  2:9395786/1‑148 (MQ=255)
                                                                                                                      ccGTTTGAGTCATTATTTGTCCCGCGGTCGTTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCACCCGTTGATGCCGGTGTATATCGATATTTTGCGTAAAGTGGTTGGGGTTTAGga                                 <  2:5055761/150‑1 (MQ=255)
                                                                                                                        gTTTGAGTCATTATTTGTCCCGCGGTCGTTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCACCCGTTGATGCCGGTGTATATCGATATTTTGCGTAAAGTGGTTGGGGTTTAGgaga                               <  1:5514387/150‑1 (MQ=255)
                                                                                                                         tttGAGTCATTATTTGTCCCGCGGTCGTTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCACCCGTTGATGCCGGTGTATATCGATATTTTGCGTAAAGTGGTTGGGGTTTAGGAGAt                              <  2:6602113/150‑1 (MQ=255)
                                                                                                                               tCATTATTTGTCCCGCGGTCGTTTGTTGTGGCTGAAGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCACCCCTTGATGCCGGTGTATATCGATATTTTTCGTAAATTGGTTTGGGTTTAGGAGATAGTAtt                        >  2:10523519/1‑150 (MQ=255)
                                                                                                                                    attTGTCCCGCGGTCGTGTGTTGTGGCTGACGCCGTTTAATTCTGCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTAGCCACCCCTTTATGCCGGTGTATATCGATATTTTGCGTAAAGTGGTTTGGGTTTAGGATATAGTCTTGTGCg                   >  2:1305938/1‑150 (MQ=255)
                                                                                                                                     tttGTCCCGCGGTCGTTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCACCCGTTGATGCCGGTGTATATCGATATTTTGCGTAAAGTGGTTGGGGTTTAGGAGATAGTCTTGTGCgg                  <  1:12240337/150‑1 (MQ=255)
                                                                                                                                     tttGTCCCGCGGTCGTTTGTTGTGGCTGACGCCGTTGATTTCTGCCAACGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCACCCCTTGATGCCGGTGTATATCGATAATTTGCGTAAAGTGGTTGGGGTTTAGGAGATAGTATTGTGCgg                  >  2:790830/1‑150 (MQ=255)
                                                                                                                                        gTCCCGCGGTCGTTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCACCCGTTGATGCCGGTGTATATCGATATTTTGCGTAAAGTGGTTGGGGTTTAGGAGATAGTCTTGTGCGGGtt               >  2:1001066/1‑150 (MQ=255)
                                                                                                                                        gTCCCGCGGTCGTTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCACCCGTTGATGCCGGTGTATATCGATATTTTGCGTAAAGTGGTTGGGGTTTAGGAGATAGTCTTGTGCGGGtt               <  2:2715293/150‑1 (MQ=255)
                                                                                                                                          cccGCGGTCGTGTGTGGGGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCACCCGTTGATGCCGGTGTAGATCGATATTTTGCGTAAAGTGGTTGGGGTTTAGGAGATAGTCTTGTGCGGGTTGc             <  1:3386929/150‑1 (MQ=255)
                                                                                                                                           ccGCGGTCGTTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTAGCCACCCGTTGATTCCGGTGTATATAGATATTTTGCGTAAAGTGGTTGGGGTTTAGGAGATAGTCTTGTGCGGGTTGcc            >  2:3924820/1‑150 (MQ=255)
                                                                                                                                               ggTCGTTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCACCCGTTGATGCCGGTGTATATCGATATTTTGCGTAAAGTGGTTGGGGTTTAGGAGATAGTCTTGTGCGGGTTGCCTGAt        <  2:12035042/150‑1 (MQ=255)
                                                                                                                                                gTCGTTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCACCCGTTGATGCCGGTGTATATCGATATTTTGCGTAAAGTGGTTGGGGTTTAGGAGATAGTCTTGTGCGGGTTGCCTGATg       <  2:7382490/150‑1 (MQ=255)
                                                                                                                                                gTCGTTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCACCCGTTGATGCCGGTGTATATCGATATTTTGCGTAAAGTGGTTGGGGTTTAGGAGATAGTCTTGTGCGGGTTGCCTGATg       <  1:1678715/150‑1 (MQ=255)
                                                                                                                                                gTCGTTTGGTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCACCCGTTGATGCCGGTGTATATCGATATTTTGCGTAAAGTGGTTGGGGTTTAGGAGATAGTCTTGTGCGGGTTGCCTGATg       <  1:858467/150‑1 (MQ=255)
                                                                                                                                                   gTTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCACCCGTTGATGCCGGTGTATATCGATATTTTGCGTAAAGTGGTTGGGGTTTAGGAGATAGTCTTGTGCGGGTTGCCTGATGCGa    >  1:4090439/1‑150 (MQ=255)
                                                                                                                                                    tttGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCACCCGTTGATTCCGGTGTATATCGATATTTTGCGTAAAGTGGTTGGGGTTTAGGAGATAGTCTTTTGCGGGTTGCCTGATGCGAc   >  1:5373704/1‑150 (MQ=255)
                                                                                                                                                    tttGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCACCCGTTGATGCCGGTGTATATCGATATTTTGCGTAAAGTGGTTGGGGTTTAGGAGATAGTCTTGTGCGGGTTGCCTGATGCGAc   >  2:4412087/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
ATATTCCTGCCGGAATGCAGAGCGGTGAGTTTAAATGGTTGCTGCTGGCAACAGCGGTGTTTTTGTGGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCGACTGACCGTTTGAGTCATTATTTGTCCCGCGGTCGTTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCACCCGTTGATGCCGGTGTATATCGATATTTTGCGTAAAGTGGTTGGGGTTTAGGAGATAGTCTTGTGCGGGTTGCCTGATGCGACG  >  NC_000913/71850‑72148

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: