Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 784,979 G→T 7.9% I113I (ATC→ATA ybgS ← putative periplasmic protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913784,9790GT 7.9% 2043.9 / 23.3 638I113I (ATC→ATAybgSputative periplasmic protein
Reads supporting (aligned to +/- strand):  ref base G (244/320);  new base T (72/2);  total (316/322)
Fisher's exact test for biased strand distribution p-value = 1.04e-21
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 649 total aligned reads displayed.

TCATCCCTTAATTATTCTCACTTAACTTTATGACATCATACTGCTTTTAGAAGTGAAAAATTAAAAGGGAGAGACTCCGCTCTCCCATTATTGGCTATTTTGCAGGGTTACTGCGTGGTACCGTCGGTTTTGGTATCGACATCATTATTGATGCCATCACCGGTTTGTACCTTTTTATTGATATCCGGACAGCGACCATCTTTGCACATGGTGTTCTTGTGCTCTTCATCTTTGGTCATTCCGTCATTGTTCATTGAAGAACCATCCGAATGCAGCATTGTGCCGCCAGAACCGGTATT  >  NC_000913/784830‑785128
                                                                                                                                                     |                                                                                                                                                     
 cATCCCTTAATTATTCTCACTTAACTTTATGACATCATACTGCTTTTAGAAGTGAAAAATTAAAAGGGAGAGACTCCGCTCTCCCATTATTGGCTATTTTGCAGGGTTACTGCGTGGTACCGTCGGTTTTGGTATCGACATCATTATTGa                                                                                                                                                      <  1:2560897/150‑1 (MQ=255)
  aTCCCTTAATTATTCTCACTTAACTTTATGACATCATACTGCTTTTAGAAGTGAAAAATTAAAAGGGAGAGACTCCGCTCTCCCATTATTGGCTATTTTGCAGGGTTACTGCGTGGTACCGTCGGTTTTGGTATCGACATCATTATTGAt                                                                                                                                                     <  1:10317950/150‑1 (MQ=255)
  aTCCCTTAATTATTCTCACTTAACTTTATGACATCATACTGCTTTTAGAAGTGAAAAATTAAAAGGGAGAGACTCCGCTCTCCCATTATTGGCTATTTTGCAGGGTTACTGCGTGGTACCGTCGGTTTTGGTATCGACATCATTATTGAt                                                                                                                                                     <  2:285337/150‑1 (MQ=255)
   tCCCTTAATTATTCTCACTTAACTTTATGACATCATACTGCTTTTAGAAGTGAAAAATTAAAAGGGAGAGACTCCGCTCTCCCATTATTGGCTATTTTGCAGGGTTACTGCGTGGTACCGTCGGGTTTGGTATCGACCTCATTATTGATg                                                                                                                                                    >  1:5403070/1‑150 (MQ=255)
    cccTTAATTATTCTCACTTAACTTTATGACATCATACTGCTTTTAGAAGTGAAAAATTAAAAGGGAGAGACTCCGCTCTCCCATTATTGGCTATTTTGCAGGGTTACTGCGTGGTACCGTCGGTTTTGGTATCGACATCATTATTGATGc                                                                                                                                                   <  1:9504985/150‑1 (MQ=255)
      cTTAATTATTCTCACTTAACTTTATGACATCATACTGCTTTTAGAAGTGAAAAATTAAAAGGGAGAGACTCCGCTCTCCCATTATTGGCTATTTTGCAGGGTTACTGCGTGGTACCGTCGGTTTTGGTATCGACATCATTATTGATGCCa                                                                                                                                                 >  1:1868530/1‑150 (MQ=255)
       ttAATTATTCTCACTTAACTTTATGACATCATACTGCTTTTAGAAGTGAAAAATTAAAAGGGAGAGACTCCGCTCTCCCATTATTGGCTATTTTGCAGGGTTACTGCGTGGTACCGTCGGTTTTGGTATCGACATCATTATTGATGCCAt                                                                                                                                                >  1:3874291/1‑150 (MQ=255)
          attattCTCACTTAACTTTATGACATCATACTGCTTTTAGAAGTGAAAAATTAAAAGGGAGAGACTCCGCTCTCCCATTATTGGCTATTTTGCAGGGTTACTGCGTGGTACCGTCGGTTTTGGTATCGACATCATTATTGATGCCATCAc                                                                                                                                             <  1:9319033/150‑1 (MQ=255)
              ttCTCACTTAACTTTATGACATCATACTGCTTTTAGAAGTGAAAAATTAAAAGGGAGAGACTCCGCTCTCCCATTATTGGCTATTTTGCAGGGTTACTGCGTGGTACCGTCGGTTTTGGTATCGACATCATTATTGATTCCATCACCGGt                                                                                                                                         >  1:9139570/1‑150 (MQ=255)
               tctcACTTAACTTTATGACATCATACTGCTTTTAGAAGTGAAAAATTAAAAGGGAGAGACTCCGCTCTCCCATTATTGGCTATTTTGCAGGGTTACTGCGTGGTACCGTCGGTTTTGGTATCGACATCATTATTGATGCCATCACCGGtt                                                                                                                                        >  1:7828215/1‑150 (MQ=255)
                ctcACTTAACTTTATGACATCATACTGCTTTTAGAAGTGAAAAATTAAAAGGGAGAGACTCCGCTCTCCCATTATTGGCTATTTTGCAGGGTTACTGCGTGGTACCGTCGGGTTTGGTATCGACATCAATATTGATGCCATcccccggtt                                                                                                                                       >  2:10395244/1‑145 (MQ=255)
                 tcACTTAACTTTATGACATCATACTGCTTTTAGAAGTGAAAAATTAAAAGGGAGAGACTCCGCTCTCCCATTATTGGCTATTTTGCAGGGTTACTGCGTGGTACCGTCGGTTTTTGTATCGACATCAATATTGATTCCATCACCGGTTTg                                                                                                                                      >  1:1747024/1‑150 (MQ=255)
                 tcACTTAACTTTATGACATCATACTGCTTTTAGAAGTGAAAAATTAAAAGGGAGAGACTCCGCTCTCCCATTATTGGCTATTTTGCAGGGTTACTGCGTGGTACCGTCGGGTTTGGTATCGACATCATTATTGTTTCCCTCACCGGtttt                                                                                                                                      >  2:11756919/1‑149 (MQ=255)
                   aCTTAACTCTATGACATCATACTGCTTTTAGAAGTGAAAAATTAAAAGGGAGAGACTCCGCTCTCCCATTATTGGCTATTTTGCAGGGTTACTGCGTGGTACCGTCGGTTTTGGTATCGACATCATTATTGATGCCATCACCGGTTTGTa                                                                                                                                    <  2:10089523/150‑1 (MQ=255)
                     ttAACTTTATGACATCATACTGCTTTTAGAAGTGAAAAATTAAAAGGGAGAGACTCCGCTCTCCCATTATTGGCTATTTTGCAGGGTTACTGCGTGGTACCGTCGGTTTTGGTATCCACATCATTATTGATGCCATCACCGGTTTGTAcc                                                                                                                                  >  2:3546894/1‑150 (MQ=255)
                      tAACTTTATGACATCATACTGCTTTTAGAAGTGAAAAATTAAAAGGGAGAGACTCCGCTCTCCCATTATTGGCTATTTTGCAGGGTTACTGCGTGGTACCGTCGGTTTTGGTATCGACATCATTATTGATGCCATCACCGGTTTGTACCt                                                                                                                                 <  1:5789860/150‑1 (MQ=255)
                      tAACTTTATGACATCATACTGCTTTTAGAAGTGAAAAATTAAAAGGGAGAGACTCCGCTCTCCCATTATTGGCTATTTTGCAGGGTTACTGCGTGGTACCGTCGGTTTTGGTATCGACATCATTATTGATGCCATCACCGGTTTGTACCt                                                                                                                                 <  1:154779/150‑1 (MQ=255)
                      tAACTTTATGACATCATACTGCTTTTAGAAGTGAAAAATTAAAAGGGAGAGACTCCGCTCTCCCATTATTGGCTATTTTGCAGGGATACAGCGTGGTACCGTCGGTTTTTGTATCGACATCAATATTTATGACATCACCCGTTTGTaaca                                                                                                                                 >  2:7411728/1‑147 (MQ=255)
                         cTTTATGACATCATACTGCTTTTAGAAGTGAAAAATTAAAAGGGAGAGACTCCGCTCTCCCATTATTGGCTATTTTGCAGGGTTACTGCGTGGTACCGTCGGTTTTGGTATCGACATCATTATTGATGCCATCACCGGTTTGTACCtttt                                                                                                                              <  1:1112409/150‑1 (MQ=255)
                            tATGACATCATACTGCTTTTAGAAGTGAAAAATTAAAAGGGAGAGACTCCGCTCTCCCATTATTGGCTATTTTGCAGGGTTACTGCGTGGTACCGTCGGTTTTGGTATCGACATCATTATTGATGCCATCACCGGTTTGTACCTTTTTAt                                                                                                                           <  2:5033419/150‑1 (MQ=255)
                                       aCTGCTTTTAGAAGTGAAAAATTAAAAGGGAGAGACTCCGCTCTCCCATTATTGGCTATTTTGCAGGGTTACTGCGTGGTACCGTCGGTTTTGGTATCGACATCATTATTGATGCCATCACCGGTTTGTACCTTTTTATTGATATCCGGa                                                                                                                <  1:2436727/150‑1 (MQ=255)
                                        cTGCTTTTAGAAGTGAAAAATTAAAAGGGAGAGACTCCGCTCTCCCATTATTGGCTATTTTGCAGGGTTACTGCGTGGTACCGTCGGTTTTGGTATCGACCTCCTTATTGATGCCATCACCGGGTTGGTCCTTTTTATTGGTATCCGGAc                                                                                                               >  2:6269403/1‑150 (MQ=255)
                                        cTGCTTTTAGAAGTGAAAAATTAAAAGGGAGAGACTCCGCTCTCCCATTATTGGCTATTTTGCAGGGTTACTGCGTGGTACCGTCGGTTTTGGTATCGACATCATTATTGATGCCATCACCGGTTTGTACCTTTTTATTGATATCCGGAc                                                                                                               <  1:3683572/150‑1 (MQ=255)
                                            ttttAGAAGTGAAAAATTAAAAGGGAGAGACTCCGCTCTCCCATTATTGGCTATTTTGCAGGGTTACTGCGTGGTACCGTCGGGGTTTGTGTCGCCATCATTTTTGATGCCAGAACCGGGTTGGACCTTTTTATTGATGTCCGGACcgcg                                                                                                           >  2:9469882/1‑150 (MQ=255)
                                             tttAGAAGTGAAAAATTAAAAGGGAGAGACTCCGCTCTCCCATTATTGGCTATTTTGCAGGGTTACTGCGTGGTACCGTCGGTTTTGGTATCGACATCATTATTGATGCCAACACCGGTTTGTACCTTTTTATTGATATCCGGACAGCGa                                                                                                          >  1:6969561/1‑150 (MQ=255)
                                                  aaGTGAAAAATTAAAAGGGAGAGACTCCGCGCTCCCATTATTGGCTATTTTGCAGGGTTACTGCGTGGTCCCGTCGGGTTTGGTATCGACAACATTCTTGTTGCCCTCACCGGGGGGTTACTTTTTATTGTTATCCGGCCCGCGGCCagc                                                                                                     >  2:10001506/1‑148 (MQ=255)
                                                     tGAAAAATTAAAAGGGAGAGACTCCGCTCTCCCATTATTGGCTATTTTGCAGGGTTACTGCGTGGTACCGTCGGTTTTGGTATCGACATCATTATTTATGCCATCACCGGTGTGTACCATTTTATTGATATACGGACAGCGACCATCttt                                                                                                  >  1:6693277/1‑150 (MQ=255)
                                                     tGAAAAATTAAAAGGGAGAGACTCCGCTCTCCCATTATTGGCTATTTTGCAGGGTTACTGCGTGGTACCGTCGGTTTTGGTATCGACATCATTATTGATGCCATCACCGGTTTGTACCTTTTTATTGATATCCGGACAGCGACCATCttt                                                                                                  <  2:5329483/150‑1 (MQ=255)
                                                      gAAAAATTAAAAGGGAGAGACTCCGCTCTCCCATTATTGGCTATTTTGCAGGGTTACTGCGTGGTACCGTCGGTTTTGGTATCGACATCATTATTGATGCCATCACCGGTTTGTACCTTTTTATTGATATCCGGACAGCGACCATCTTTg                                                                                                 <  2:3812272/150‑1 (MQ=255)
                                                      gAAAAATTAAAAGGGAGAGACTCCGCTCTCCCATTATTGGCTATTTTGCAGGGTTACTGCGTGGTACCGTCGGTTTTGGTATCGACATCATTATTGATGCCATCACCGGTTTGTACCTTTTTATTGATATCCGGACAGCGACCATCTTTg                                                                                                 <  2:8218895/150‑1 (MQ=255)
                                                      gAAAAATTAAAAGGGAGAGACTCCGCTCTCCCATTATTGGCTATTTTGCAGGGTTACTGCGTGGTACCGTCGGTTTTGGTATCGACATCATTATTGATGCCATCACCGGTTTGTACCTTTTTATTGATATACGGACAGCGACCATCTTTg                                                                                                 >  1:11519331/1‑150 (MQ=255)
                                                      gAAAAATTAAAAGGGAGAGACTCCGCTCTCCCATTATTGGCTATTTTGCAGGGTTACTGCGTGGTACCGTCGGGTTTTGTATCGACATCATTATTGTTGCCATCACCGGTTTGTACCTTTTTATTTATATACGGACAGCGACCATCtttt                                                                                                 >  1:10600289/1‑149 (MQ=255)
                                                       aaaaaTTAAAAGGGAGAGACTCCGCTCTCCCATTATTGGCTATTTTGCAGGGTTACTGCGTGGTACCGTCGGGTTTGGTATCGACATCATTATTGATGCCATCACCGGTGTGTACCTTTTTATTGGTATCCGGACAGCGACCATCTTTGc                                                                                                >  1:3541344/1‑150 (MQ=255)
                                                        aaaaTTAAAAGGGAGAGACTCCGCTCTCCCATTATTGGCTATTTTGCAGGGTTACTGCGTGGTACCGTCGGTTTTGGTATCGACATCATTATTGATGCCATCACCGGTTTGTACCTTTTTATTGTTATCCGGACAGCGACCATCTTTGca                                                                                               >  1:8065966/1‑150 (MQ=255)
                                                            ttAAAAGGGAGAGACTCCGCTCTCCCATTATTGGCTATTTTGCAGGGTTACTGCGTGGTACCGTCGGTTTTGGTATCGACATCAAAATTGATGCCATCACCGGTTTGTACCTTTTTATTGATATCCGGACAGCGACCAACTTTGCACATg                                                                                           >  2:3954778/1‑150 (MQ=255)
                                                               aaaGGGAGAGACTCCGCTCTCCCATTATTGGCTATGTTGCTGGGTTACTGCGTGGTACCGTCGGTTTTGGTATCGACATCATTATTGATTCCATCACCGGTTTGTACCTTTTTATTGATATCCGGACAGCGACCATCTTTGCACATGgtg                                                                                        <  1:5959741/150‑1 (MQ=255)
                                                                 aGGGAGAGACTCCGCTCTCCCATTATTGGCTATTTTGCAGGGTTACTGCGTGGTACCGTCGGTTTTGGTATCGACATCATTATTGATGCCATCACCGGTTTGTACCTTTTTATTGATATCCGGACAGCGACCATCTTTGCACATGGTGtt                                                                                      <  1:8735826/150‑1 (MQ=255)
                                                                 aGGGAGAGACTCCGCTCTCCCAGTATTGGCTCTTTTGCAGGGTTACTGCGTGGTACCGTCGGGTTTGGTATCCACAACCGTAATGATGCCAGCACCGCGTTGTACCCTTTTCTTGTTATCCGGGCAGCGCCCCGCCTTGCACCGGGTGtt                                                                                      >  2:2214183/1‑150 (MQ=255)
                                                                  gggAGAGACTCCGCTCTCCCATTATTGGCTATTTTGCAGGGTTACTGCGTGGTACCGTCGGTTTTGGTGTCGACATAATTATTGTTGCCATCACCGGTTTTTACCTTTTTATTTATATCCGGACAGCGACCATCTTTGGACAAGGTGttg                                                                                     >  2:11511644/1‑149 (MQ=255)
                                                                  gggAGAGACTCCGCTCTCCCATTATTGGCTATTTTGCAGGGTTACTGCGTGGTACCGTCGGTTTTGGTATCGACATCATTATTGATGCCATCACCGGTTTGTACCTTTTTATTGATATCCGGACAGCGACCATCTTTGCACATGGTGTTc                                                                                     <  1:7138414/150‑1 (MQ=255)
                                                                  gggAGAGACTCCGCTCTCCCATTATTGGCTATTTTGCAGGGTTACTGCGTGGTACCGTCGGTTTTGGTATCGACATCATTATTGATGCCATCACCGGTTTGTACCTTTTTATTGATATCCGGACAGCGACCATCTTTGCACATGGTGTTc                                                                                     <  1:3266700/150‑1 (MQ=255)
                                                                   ggAGAGACTCCGCTCTCCCATTATTGGCTATTTTGCAGGGTTACTGCGTGGTACCGTCGGTTTTGGTATCGACATCATTATTTTTGCCATCAACGGTTTGTACCTTTTTATTTATATCCGGACAGCGACCATATTTGCACCTGGTGTTCt                                                                                    >  2:7757321/1‑150 (MQ=255)
                                                                   ggAGAGACTCCGCTCTCCCATTATTGGCTATTTTGCAGGGTTACTGCGTGGTACCGTCGGTTTTGGTATAGACAACAATATTTATGCCATCACCGGGTTGTAACATTTTATTGATATCCGGACAGCGACCATCGTTGCACATGGTGTTCt                                                                                    >  2:439309/1‑150 (MQ=255)
                                                                     agagaCTCCGCTCTCCCATTATTGGCTATTTTGCAGGGTTACTGCGTGGTACCGTCGGTTTTGGTATCGACATCATTATTGATGCCATCACCGGTTTGTACCTTTTTATTGATATCCGGACAGCGACCATCTTTGCACATGGTGTTCTtg                                                                                  <  2:7128573/150‑1 (MQ=255)
                                                                       agaCTCCGCTCTCCCATTATTGGCTATTTTGCAGGGTTACTGCGTGGTACCGTCGGTTTTTGTATCGACATCATTATTTATGCCATCACCCGTTTTTAACTTTTTATTTATATAAGGACAGCGACCAAATTTTCACATGGTGTTATtgtg                                                                                >  1:4200031/1‑150 (MQ=255)
                                                                       agaCTCCGCTCTCCCATTATTGGCTATTTTGCAGGGTTACTGCGTGGTACCGTCGGTTTTGGTATCGACATCATTATTGATGCCATCACCGGTTTGTACCTTTTTATTGATATCCGGACAGCGACCATCTTTGCACATGGTGTTCTtgtg                                                                                <  1:8979799/150‑1 (MQ=255)
                                                                       agaCTCCGCTCTCCCATTATTGGCTATTTTGCAGGGTTACTGCGTGGTACCGTCGGTTTTGGTATCGACATCATTATTGATGCCATCACCGGTTTGTACCTTTTTATTGATATCCGGACAGCGACCATCTTTGCACATGGTGTTCTtgtg                                                                                <  2:8543888/150‑1 (MQ=255)
                                                                           tCCGCTCTCCCATTATTGGCTATTTTGCAGGGTTACTGCGTGGTACCGTCGGTTTTTGTATAGACATCAATATTGATGCCATCACCGGTTTGTACAATTTTATTTATATCCGGACAGCGACCATCTTTTCACATGGTTTTCTTGTGctat                                                                            >  2:11367226/1‑148 (MQ=255)
                                                                            ccGCTCTCCCATTATTGGCTATTTTGCAGGGTTACTGCGTGGTACCGTCGGTTTTGGTATCGACATCATTATTGATGCCATCACCGGTTTGTACCTTTTTATTGATATCCGGACAGCGACCATCTTTGCACATGGTGTTCTTGTGCTCtt                                                                           <  1:6527737/150‑1 (MQ=255)
                                                                                tctcCCATTATTGGCTATTTTGCAGGGTTACTGCGTGGTACCGTCGGTTTTTGTATAGACAACAATATTTATGCCATCACCGGTTTTTACCTTTTTATTTATATCCGGACAGCCACCAACTTTGCACATGGTGTTCTTTTGCTCTTCATc                                                                       >  2:3398504/1‑150 (MQ=255)
                                                                                 ctcCCATTATTGGCTATTTTGCAGGGTTACTGCGTGGTACCGTCGGTTTTGGTATCGACATCATTATTGATGCCATCACCGGTTTGTACCTTTTTATTGATATCCGGACAGCGACCATCTTTGCACATGGTGTTCTTGTGCTCTTCATCt                                                                      <  1:2878890/150‑1 (MQ=255)
                                                                                  tcCCATTATTGGCTATTTTGCAGGGTTACTGCGTGGTACCGTCGGTTTTGGTAACGACATAAATATTGATGCAATCACCCGTTTGTACCATTTTATTTATATCAGTAACGCGCCCATGTTTTCACATTGTGTTATTGTGCTCTTAATCtt                                                                     >  2:4779778/1‑150 (MQ=255)
                                                                                   cccATTATTGGCTATTTTGCAGGGTTACTGCGTGGTACCGTCGGTTTTTGTATCGACATCAATATTTATGCCATCACCCGTTTTTACCTTTTTATTGAAATACGGACAGCGACCAACATTTAACATGGTGTTCTTGTGCTCTTCATCatt                                                                    >  2:11994976/1‑150 (MQ=255)
                                                                                    ccATTATTGGCTATTTTGCAGGGTTACTGCGTGGTACCGTCGGTTTTGGTATCGACATCATTATTGATGCCATCACCGGTTTGTACCTTTTTATTGATATCCGGACAGCGACCATCTTTGCACATGGTGTTCTTGTGCTCTTCATCTTTg                                                                   <  2:4960239/150‑1 (MQ=255)
                                                                                     cATTATTGGCTATTTTGCAGGGTTACTGCGTGGTACCGTCGGTTTTGGTATCGACATCAATATTTATGCCAACACCCGTTTGTACCCTTTTATTTATATACCGACACCGACCATCATTTCACATGGTGTTCTTGTGCTCTTCATAttttg                                                                  >  1:4033273/1‑148 (MQ=255)
                                                                                     cATTATTGGCTATTTTGAAGGGTTACTGCGTGGTACCGTCGGTTTTGGTATCGACATCATTATTGATTCCATCACCGGTTTGTACCTTTTTATTGATATCCGGACAGCGAACAACATTGCACATGGTGTTCTTGTTCTATTCATCttttg                                                                  >  2:11190425/1‑148 (MQ=255)
                                                                                      attattGGCTATTTTGCAGGGTTACTGCGTGGTACCGTCGGTTTTGGTATCGACATCAATATTGATTCCATAACCGGTTTTTACCTTTTTATTTATATCCGGACAGCGACCAAATTTTCACAAGGTGTTCTTGTGCTCTTAATATTTTGt                                                                 >  2:5810070/1‑150 (MQ=255)
                                                                                      attattGGCTATTTTGCAGGGTTACTGCGTGGTACCGTCGGTTTTGGTATAGACAACAATATTGATGCCATCAACGGTTTGTACCTTTTTATTTTTATACGGACAGCGACCATCTTTGCACATGGTGTTCTTTTGCTCTTCATCTTTGGt                                                                 >  1:8547144/1‑150 (MQ=255)
                                                                                          ttGGCTATTTTGCAGGGTTACTGCGTGGTACCGTCGGTTTTGGTATCGACATCATTATTGATGCCATCACCGGTTTGTACCTTTTTATTGATATCCGGACAGCGACCATCTTTGCACATGGTGTTCTTGTGCTCTTCATCTTTGGTCAtt                                                             <  2:8062753/150‑1 (MQ=255)
                                                                                          ttGGCTATTTTGCAGGGTTACTGCGTGGTACCGTCGGTTTTGGTATCGACATCATTATTGATGCCATCACCGGTTTGTACCTTTTTATTGATATCCGGACAGCGACCATCTTTGCACATGGTGTTCTTGTGCTCTTCATCTTTGGTCAtt                                                             <  2:3177932/150‑1 (MQ=255)
                                                                                           tGGCTATTTTGCAGGGTTACTGCGTGGTACCGTCGGTTTTGGTATCGACATCATTATTGATGCCATCACCGGTTTGTACCTTTTTATTGATATCCGGACAGCGACCATCTTTGCACATGGTGTTCTTGTGCTCTTCATCTTTGGTCATTc                                                            <  2:1868530/150‑1 (MQ=255)
                                                                                             gCTATTTTGCAGGGTTACTGCGTGGTACCGTCGGTTTTGGTATAGACATCATTAATTATTCCATCACCCGTTTGTACCTATTTATTGATATACGGACAGCGACCATCTTTGCACATCGTGATCTTGTGCTCTTCATCTTTGGTCATTCCg                                                          >  2:6955889/1‑150 (MQ=255)
                                                                                              cTATTTTGCAGGGTTACTGCGTGGTACCGTCGGTTTTGGTATCGACATCATTATTGATGCCATCACCCGTTTGTACCATTTTATTTATATCCGGACAGCGACCAACTTTGCACATGGTGTTCTTTTGCTCTTCATCTTTGGTCATTACGt                                                         >  2:134574/1‑150 (MQ=255)
                                                                                                aTTTTGCAGGGTTACTGCGTGGTACCGTCGGTTTTGGTATCGACATCATTATTGATGCCATCACCGGTTTGTACCTTTTTATTGATATCCGGACAGCGACCATCTTTGCACATGGTGTTCTTGTGCTCTTCATCTTTGGTCATTCCGtta                                                       <  1:5024090/150‑3 (MQ=255)
                                                                                                  tttGCAGGGTTACTGCGTGGTACCGTCGGTTTTGGTATCGACATCATTATTGATGCCATCACCGGTTTGTACCTTTTTATTGATATCCGGACAGCGACCATCTTTGCACATGGTGTTCTTGTGCTCTTCATCTTTGGTCATTCCGTCAtt                                                     <  2:3654136/150‑1 (MQ=255)
                                                                                                        gggTTACTGCGTGGTACCGTCGGTTTTGGTATCGACATCATTATTGATGCCATCACCGGTTTGTACCTTTTTATTGATATCCGGACAGCGACCATCTTTGCACATGGTGTTCTTGTGCTCTTCATCTTTGGTCATTCCGTCATTGTTCAt                                               <  2:6449816/150‑1 (MQ=255)
                                                                                                         ggTTACTGCGTGGTACCGTCGGTTTTTGTATCGACATCATTATTTATGCCATCACCCGGTTGTAACTTTTTATTGATATCCGGACAGCGACCATCTTTGCACATGGGGTTCTTGTGCTCTTCATCTTTGGTCAATCCGTCATTGTTCAtt                                              >  2:2511405/1‑150 (MQ=255)
                                                                                                         ggTTACTGCGTGGTACCGTCGGTTTTGGTATCGACATCATTATTTATGCCATAACCGGTTTGTACCATTTTATTGATATCCGGACAGCGACCATAATTGCACATTGTGTTCTTGTGCTCTTCATATTTGGTCATTACCTCATTGTTAAtt                                              >  2:9158342/1‑150 (MQ=255)
                                                                                                         ggTTACTGCGTGGTACCGTCGGTTTTGGTATCGACATCATTATTGATGCCATCACCGGTTTGTACCTTTTTATTGATATCCGGACAGCGACCATCTTTGCACATGGTGTTCTTGTGCTCTTCATCTTTGGTCATTCCGTCATTGTTCAtt                                              <  1:11767368/150‑1 (MQ=255)
                                                                                                               tGCGTGGTACCGTCGGTTTTGGTATCGACATCATTATTGATGCCATCACCGGTTTGTACCTTTTTATTGATATCCGGACAGCGACCATCTTTGCACATGGTGTTCTTGTGCTCTTCATCTTTGGTCATTCCGTCATTGTTCATTgaagaa                                        <  2:8761290/150‑1 (MQ=255)
                                                                                                                gCGTGGTACCGTCGGTTTTGGTATCGACATCATTATTGATGCCATCACCGGTTTGTACCTTTTTATTGATATCCGGACAGCGACCATCTTTGCACATGGTGTTCTTGTGCTCTTCATCTTTGGTCATTCCGTCATTGTTCATTGAAGAAc                                       <  1:4615062/150‑1 (MQ=255)
                                                                                                                  gTGGTACCGTCGGTTTTGGTATCGACATCATTATTGATTCCATCACCGGTTTGTACCTTTTTATTGATATCCGGACAGCGACCAACTTTGCACATGGTTTTCTTGTGCTCTTCATCGTTGGTCATTACGTCATTGTTAATTGAATAACCa                                     >  2:1619625/1‑150 (MQ=255)
                                                                                                                    ggTACCGTCGGTTTTGGTATTGACATCATTATTGATGCCATCACCGGTTTGTACCTTTTTATTGATATCCGGACAGCGACCATCTTTGCACATGGTGTTCTTGTGCTCTTCATCTTTGGTCATTCCGTCATTGTTCATTGAAGAACCATc                                   <  2:7893209/150‑1 (MQ=255)
                                                                                                                    ggTACCGTCGGTTTTGGTATCGACATCATTATTGATGCCATCACCGGTTTGTACCTTTTTATTGATATCCGGACAGCGACCATCTTTGCACATGGTGTTCTTGTGCTCTTCATCTTTGGTCATTCCGTCATTGTTCATTGAAGAACCATc                                   <  2:4126177/150‑1 (MQ=255)
                                                                                                                     gTACCGTCGGTTTTGGTATCGACATCATTATTGATGCCATCACCGGTTTGTACCTTTTTATTGATATCCGGACAGCGACCATCTTTGCACATGGTGTTCTTGTGCTCTTCATCTTTGGTCATTCCGTCATTGTTCATTGAAGAACCATcc                                  <  1:8314195/150‑1 (MQ=255)
                                                                                                                          gTCGGTTTTGGTATCGACATCATTATTGATGCCATCACCGGTTTGTACCTTTTTATTGATATCCGGACAGCGACCATCTTTGCACATGGTGTTCTTGTGCTCTTCATCTTTGGTCATTCCGTCATTGTTCATTGAAGAACCATCCGAATg                             >  2:5697010/1‑150 (MQ=255)
                                                                                                                          gTCGGTTTTGGTATCGACATCATTATTGATGCCATCACCGGTTTGTACCTTTTTATTGATATCCGGACAGCGACCATCTTTGCACATGGTGTTCTTGTGCTCTTCATCTTTGGTCATTCCGTCATTGTTCATTGAAGAACCATCCGAATg                             >  2:1227272/1‑150 (MQ=255)
                                                                                                                          gTCGGTTTTGGTATCGACATCATTATTGATGCCATCACCGGTTTGTACCTTTTTATTGATATCCGGACAGCGACCATCTTTGCACATGGTGTTCTTGTGCTCTTCATCTTTGGTCATTCCGTCATTGTTCATTGAAGAAACATCCGAATg                             >  2:90418/1‑150 (MQ=255)
                                                                                                                            cGGTTTTGGTATCGACATCATTATTGATGCCATCACCGGTTTGTACCTTTTTATTGATATCCGGACAGCGACCATCTTTGCACATGGTGTTCTTGTGCTCTTCATCTTTGGTCATTCCGTCATTGTTCATTGAAGAACCATCCGAATgca                           <  2:1555271/150‑1 (MQ=255)
                                                                                                                               ttttGGTATCGACATCATTATTGATGCCATCACCGGTTTGTACCTTTTTATTGATATCCGGACAGCGACCATCTTTGCACATGGTGTTCTTGTGCTCTTCATCTTTGGTCATTCCGTCATTGTTCATTGAAGAACCATCCGAATgcagca                        >  1:9686640/1‑150 (MQ=255)
                                                                                                                                    gTATCGACATCATTATTGATGCCATCACCGGTTTGTACCTTTTTATTGATATCCGGACAGCGACCATCTTTGCACATGGTGTTCTTGTGCTCTTCATCTTTGGTCATTCCGTCATTGTTCATTGAAGAACCATCCGAATGCAGCATtgtg                   >  1:6125901/1‑150 (MQ=255)
                                                                                                                                     tATCGACATCATTATTGATGCCATCACCGGTTTGTACCTTTTTATTGATATCCGGACAGCGACCATCTTTGCACATGGTGTTCTTGTGCTCTTCATCTTTGGTCATTCCGTCATTGTTCATTGAAGAACCATCCGAATGCAGCATTGTgc                  >  1:10040993/1‑150 (MQ=255)
                                                                                                                                      aTCGACATCTTTATTGATGCCATCACCGGTTTGTACCTTTTTATTGATATCCGGACAGCGACCATCTTTGCACATGGTGTTCTTGTGCTCTTCATCTTTGGTCATTCCGTCATTGTTCATTGAAGAACCATCCGAATGCAGCATTGTgcc                 <  1:11412332/150‑1 (MQ=255)
                                                                                                                                      aTCGACATCATTATTGATGCCATCACCGGTTTGTACCTTTTTATTGATATCCGGACAGCGACCATCTTTGCACATGGTGTTCTTGTGCTCTTCATCTTTGGTCATTCCGTCATTGTTCATTGAAGAACCATCCGAATGCAGCATTGTgcc                 >  2:5953567/1‑150 (MQ=255)
                                                                                                                                      aTCGACATCATTATTGATGCCATCACCGGTTTGTACCTTTTTATTGATATCCGGACAGCGACCATCTTTGCACATGGTGTTCTTGTGCTCTTCATCTTTGGTCATTCCGTCATTGTTCATTGAAGAACCATCCGAATGCAGCATTGTgcc                 <  1:7947908/150‑1 (MQ=255)
                                                                                                                                       tCGACATCATTATTGATGCCATCACCGGTTTGTACCTTTTTATTGATATCCGGACAGCGACCATCTTTGCACATGGTGTTCTTGTGCTCTTCATCTTTGGTCATTCCGTCATTGTTCATTGAAGAACCATCCGAATGCAGCATTGTgccg                >  1:5556612/1‑150 (MQ=255)
                                                                                                                                        cGACATCATTATTGATGCCATCACCGGTTTGTACCTTTTTATTGATATCCGGACAGCGACCATCTTTGCACATGGTGTTCTTGTGCTCTTCATCTTTGGTCATTCCGTCATTGTTCATTGAAGAACCATCCGAATGCAGCATTGTgccgc               >  1:9768385/1‑150 (MQ=255)
                                                                                                                                         gACATCATTATTGATGCCTTCAGCGGTTTGTACCTTTTTATTGATATCCGGACAGCGACCATCTTTGCACATGGTGTTCTTGTGCTCTTCATCTTTGGTCATTCCGTCATTGTTCATTGAAGAACCATCCGAATGCAGCATTGTgccgcc              <  2:6759386/150‑1 (MQ=255)
                                                                                                                                         gACATCATTATTGATGCCATCACCGGTTTGTACCTTTTTATTGATATCCGGACAGCGACCATCTTTGCACGTGGTGTTCTTGTGCTCTTCATCTTTGGTCATTCCGTCATTGTTCATTGAAGAACCATCCGAATGCAGCATTGTgccgcc              >  2:6640231/1‑150 (MQ=255)
                                                                                                                                         gACATCATTATTGATGCCATCACCGGTTTGTACCTTTTTATTGATATCCGGACAGCGACCATCTTTGCACATGGTGTTCTTGTGCTCTTCATCTTTGGTCATTCCGTCATTGTTCATTGAAGAACCATCCGAATGCAGCATTGTgccgcc              >  1:2106956/1‑150 (MQ=255)
                                                                                                                                          aCATCATTATTGATGCCATCACCGGTTTGTACCTTTTTATTGATATCCGGACAGCGACCATCTTTGCACATGGTGTTCTTGTGCTCTTCATCTTTGGTCATTCCGTCATTGTTCATTGAAGAACCATCCGAATGCAGCATTGTGCCGCCa             <  2:9884238/150‑1 (MQ=255)
                                                                                                                                           catcatTATTGATGCCATCACCGGTTTGTACCTTTTTATTGATATCCGGACAGCGACCATCTTTGCACATGGTGTTCTTGTGCTCTTCATCTTTGGTCATTCCGTCATTGTTCATTGAAGAACCATCCGAATGCAGCATTGTGCCGCCAg            <  1:6429090/150‑1 (MQ=255)
                                                                                                                                             tcatTATTGATGCCATCACCGGTTTGTACCTTTTTATTGATATCCGGACAGCGACCATCTTTGCACAGGGTGTTCTTGTGCTCTTCATCTTTGGTCATTCCGTCATTGTTCATTGAAGAACCATCCGAATGCAGCATTGTGCCGCCAGaa          >  2:10688438/1‑150 (MQ=255)
                                                                                                                                                ttattGATGCCATCACCGGTTTGTACCTTTTTATTGATATCCGGACAGCGACCATCTTTGCACATGGTGTTCTTGTGCTCTTCATCTTTGGTCATTCCGTCATTGTTCATTGAAGAACCATCCGAATGCAGCATTGTGCCGCCAGAACCg       <  2:547823/150‑1 (MQ=255)
                                                                                                                                                ttattGATGCCATCACCGGTTTGTACCTTTTTATTGATATCCGGACAGCGACCATCTTTGCACATGGTGTTCTTGTGCTCTTCATCTTTGGTCATTCCGTCATTGTTCATTGAAGAACCATCCGAATGCAGCATTGTGCCGCCAGAACCg       >  1:7356636/1‑150 (MQ=255)
                                                                                                                                                ttattGATGCCATCACCGGTTTGTACCTTTTTATTGATATCCGGACAGCGACCATCTTTGCACATGGTGTTCTTGTGCTCTTCATCTTTGGTCATTCCGTCATTGTTCATTGAAGAACCATCCGAATGCAGCATTGTGCCGCCAGAACCg       <  1:4391595/150‑1 (MQ=255)
                                                                                                                                                  attGATGCCATCACCGGTTTGTACCTTTTTATTGATATCCGGACAGCGACCATCTTTGCACATGGTGTTCTTGTGCTCTTCATCTTTGGTCATTCCGTCATTGTTCATTGAAGAACCATCCGAATGCAGCATTGTGCCGCCAGAACCGGt     <  1:12029793/150‑1 (MQ=255)
                                                                                                                                                   ttGATGCCATCACCGGTTTGTACCTTTTTATTGATATCCGGACAGCGACCATCTTTGCACATGGTGTTCTTGTGCTCTTCATCTTTGGTCATTCCGTCATTGTTCATTGAAGAACCATCCGAATGCAGCATTGTGCCGCCAGAACCGGTa    <  1:10880363/150‑1 (MQ=255)
                                                                                                                                                   ttGATGCCATCACCGGTTTGTACCTTTTTATTGATATCCGGACAGCGACCATCTTTGCACATGGAGTTCTTGTGCTCTTCATCTTTGGTCATTCCGTCATTGTTCATTGAAGAACCATCCGAATGCAGCATTGTGCCGCCAGAACCGGTa    >  2:10221307/1‑150 (MQ=255)
                                                                                                                                                     gATGCCATCACCGGTTTGTACCTTTTTATTGATATCCGGACAGCGACCATCTTTGCACATGGTGTTCTTGTGCTCTTCATCTTTGGTCATTCCGTCATTGTTCATTGAAGAACCATCCGAATGCAGCATTGTGCCGCCAGAACCGGTAtt  >  1:4797520/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
TCATCCCTTAATTATTCTCACTTAACTTTATGACATCATACTGCTTTTAGAAGTGAAAAATTAAAAGGGAGAGACTCCGCTCTCCCATTATTGGCTATTTTGCAGGGTTACTGCGTGGTACCGTCGGTTTTGGTATCGACATCATTATTGATGCCATCACCGGTTTGTACCTTTTTATTGATATCCGGACAGCGACCATCTTTGCACATGGTGTTCTTGTGCTCTTCATCTTTGGTCATTCCGTCATTGTTCATTGAAGAACCATCCGAATGCAGCATTGTGCCGCCAGAACCGGTATT  >  NC_000913/784830‑785128

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: