Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 843,196 T→G 13.8% F289V (TTT→GTT)  rlmF → 23S rRNA m(6)A1618 methyltransferase, SAM‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913843,1960TG13.8% 1407.3 / 72.9 545F289V (TTT→GTT) rlmF23S rRNA m(6)A1618 methyltransferase, SAM‑dependent
Reads supporting (aligned to +/- strand):  ref base T (139/319);  new base G (85/2);  total (224/321)
Fisher's exact test for biased strand distribution p-value = 2.76e-35
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 557 total aligned reads displayed.

GAGCAAAGGCTTCGCGAAGCAGGTGATGTGGTTTACATCACTGGTATCTCGTGGTGAAAACTTACCGCCGTTGTATCGTGCCCTGACGGACGTGGGCGCGGTGAAGGTGGTTAAAAAAGAGATGGCCCAGGGGCAAAAGCAGAGTCGCTTTATTGCCTGGACCTTTATGAACGACGAGCAGCGCCGCCGTTTTGTCAATCGCCAGCGTTAAAGCGTTGGTTCCCCCGGCGGTAACGGTTCAGCCGGGGTCGCGCCCGGAGCAGGCAGCACCTGATAAGTCTGCACTGGCGCGCGAACG  >  NC_000913/843048‑843345
                                                                                                                                                    |                                                                                                                                                     
 agCAACGGCTTCGCGAAGCAGGTGATGTGGTTTACATCACTGGTATCTCGTGGTGAAAACTTACCGCCGTTGTATCGTGCCCTGACGGACGTGGGCGCGGTGAAGGTGGTTAAAAAAGAGATGGCCCAGGGGCAAAAGCAGAGTCGCttt                                                                                                                                                     <  2:11603698/150‑1 (MQ=255)
 agCAAAGGCTTCGCGAAGCAGGTGATGTGGTTTACATCACTGGTATCTCGTGGTGAAAACTTACCGCCGTTGTATCGTGCCCTGACGGACGTGGGCGCGGTGAAGGTGGTTAAAAAAGAGATGGCCCAGGGGCAAAAGCAGAGTCGCttt                                                                                                                                                     >  1:334407/1‑150 (MQ=255)
   cAAAGGCTTCGCGAAGCAGGTGATGTGGTTTACATCACTGGTATCTCGTGGTGAAAACTTACCGCCGTTGTATCGTGCCCTGACGGACGTGGGCGCGGTGAAGGTGGTTAAAAAAGAGATGGCCCAGGGGCAAAAGCAGAGTCGCTTTAt                                                                                                                                                   >  2:7439021/1‑150 (MQ=255)
    acaGGCTTCGCGAAGCAGGGGATGTGGTTTACATCACTGGTATCTCGTGGTGAAAACTTACCGCCGTTGTATCGTGCCCTGACGGACGTGGGCGCGGTGAAGGTGGTTAAAAAAGAGATGGCCCAGGGGCAAAAGCAGAGTCGCTTTAtt                                                                                                                                                  <  1:4433245/148‑1 (MQ=255)
    aaaGGCTTCGCGAAGCAGGTGATGTGGTTTACATCACTGGTATCTCGTGGTGAAAACTTACCGCCGTTGTATCGTGCCCTGACGGACGTGGGCGCGGTGAAGGTGGTTAAAAAAGAGATGGCCCAGGGGCAAAAGCAGAGTCGCTTTAtt                                                                                                                                                  >  2:8966074/1‑150 (MQ=255)
     aaGGCTTCGCGAAGCAGGTGATGTGGTTTACATCACTGGTATCTCGTGGTGAAAACTTACCGCCGTTGTATCGTGCCCTGACGGACGTGGGCGCGGTGAAGGTGGTTAAAAAAGAGATGGCCCAGGGGCAAAGGCAGAGTCGCGTTATTg                                                                                                                                                 >  1:6937148/1‑150 (MQ=255)
     aaGGCTTCGCGAAGCAGGTGATGTGGTTTACATCACTGGTATCTCGTGGTGAAAACTTACCGCCGTTGTATCGTGCCCTGACGGACGTGGGCGCGGTGAAGGTGGTTAAAAAAGAGATGGCCCAGGGGCAAAAGCAGAGTCGCTTTATTg                                                                                                                                                 >  2:3004179/1‑150 (MQ=255)
     aaGGCTTCGCGAAGCAGGTGATGTGGTTTACATCACTGGGATCTCGTGGTGAAAACTTACCGCCGTTGTATCGTGCCCTGACGGACGTGGGCGCGGTGAAGGTGGTTAAAAAAGAGATGGCCCAGGGGCAAAAGCAGAGTCGCTTTATTg                                                                                                                                                 <  1:10763920/150‑1 (MQ=255)
      cggCTGCGCGCAGCAGGTGATGTGGTTTACATCACTGGGATCTCGTGGTGAAAACGTACCGCCGTTGTATCGTGCCCTGACGGACGTGGGCGCGGTGAAGGTGGTTAAAAAAGAGATGGCCCAGGGGCAAAAGCAGAGTCTCTTTATTGc                                                                                                                                                <  2:9423436/149‑1 (MQ=255)
       ggCTTCGCGAAGCAGGTGATGTGGTTTACATCACTGGTATCTCGTGGTGAAAACTTACCGCCGTTGTATCGTGCCCTGACGGACGTGGGCGCGGTGAAGGTGGTTAAAAAAGAGATGGCCCAGGGGCAAAAGCAGAGTCGCTTTATTGcc                                                                                                                                               <  2:11458479/150‑1 (MQ=255)
        gCTTCGCGAAGCAGGTGATGTGGTTTACATCACTGGTATCTCGTGGTGAAAACTTACCGCCGTTGTATCGTGCCCTGACGGACGTGGGCGCGGTGAAGGTGGTTAAAAAAGAGATGGCCCAGGGGCAAAAGCAGAGTCGCTTTATTGCCt                                                                                                                                              <  1:1544419/150‑1 (MQ=255)
        gCTTCGCGAAGCAGGTGATGTGGTTTACATCACTGGTATCTCGTGGTGAAAACTTACCGCCGTTGTATCGTGCCCTGACGGACGTGGGCGCGGTGAAGGTGGTTAAAAAAGAGATGGCCCAGGGGCAAAAGCAGAGTCGCTTTATTGCCt                                                                                                                                              <  1:8033952/150‑1 (MQ=255)
          ttCGCGAAGCAGGTGATGTGGTTTACATCACTGGTATCTCGTGGTGAAAACTTACCGCCGTTGTATCGTGCCCTGACGGACGTGGGCGCGGTGAAGGTGGTTAAAAAAGAGATGGCCCAGGGGCAAAAGCAGAGTCGCTTTATTTCCTgg                                                                                                                                            >  1:9682575/1‑150 (MQ=255)
              cgAAGCAGGTGATGTGGTTTACATCACTGGTATCTCGTGGTGAAAACTTACCGCCGTTGTATCGTGCCCTGACGGACGTGGGCGCGGTGAAGGTGGTTAAAAAAGAGATGGCCCAGGGGCAAAAGCAGAGTCGCTTTATTGCCTGGACCt                                                                                                                                        <  2:11925719/150‑1 (MQ=255)
                aaGCAGGTGATGTGGTTTACATCACTGGTATCTCGTGGTGAAAACTTACCGCCGTTGTATCGTGCCCTGACGGACGTGGGCGCGGTGAAGGTGGTTAAAAAAGAGATGGCCCAGGGGCAAAAGCAGAGTCGCTTTATTGCCTGGACCttt                                                                                                                                      <  1:9295769/150‑1 (MQ=255)
                  gCAGGTGATGTGGTTTACATCACTGGTATCTCGTGGTGAAAACTTACCGCCGTTGTATCGTGCCCTGACGGACGTGGGCGCGGTGAAGGTGGTTAAAAAAGAGATGGCCCAGGGGCAAAAGCAGAGTCGCTTTATTGCCTGGACCTTTAt                                                                                                                                    <  2:1915763/150‑1 (MQ=255)
                    aGGTGATGTGGTTTACATCACTGGTATCTCGTGGTGAAAACTTACCGCCGTTGTATCGTGCCCTGACGGACGTGGGCGCGGTGAAGGTGGTTAAAAAAGAGATGGCCCAGGGGCAAAAGCAGAGTCGCTTTATTGCCTGGACCTTTATGa                                                                                                                                  <  1:12197085/150‑1 (MQ=255)
                       tGATGTGGTTTACATCACTGGTATCTCGTGGTGAAAACTTACCGCCGTTGTATCGTGCCCTGACGGACGTGGGCGCGGTGAAGGTGGTTAAAAAAGAGATGGCCCAGGGGCAAAAGCAGAGTCGCTTTATTTCCAGGACCGTTATTAacg                                                                                                                               >  1:2679891/1‑150 (MQ=255)
                        gATGTGGTTTACATCACTGGTATCTCGTGGTGAAAACTTACCGCCGTTGTATCGTGCCCTGACGGACGTGGGCGCGGTGAAGGTGGTTAAAAAAGAGATGGCCCAGGGGCAAAAGCAGAGTCGCTTTATTGCCTGGACCTTTATGAacga                                                                                                                              <  1:1874807/150‑1 (MQ=255)
                         aTGTGGTTTACATCACTGGTATCTCGTGGTGAAAACTTACCGCCGTTGTATCGTGCCCTGACGGACGTGGGCGCGGTGAAGGTGGTTAAAAAAGAGATGGCCCAGGGGCAAAAGCAGAGTCGCTTTATTGCCTGGACCTTTATGAacgac                                                                                                                             <  2:209853/150‑1 (MQ=255)
                         aTGTGGTTTACATCACTGGTATCTCGTGGTGAAAACTTACCGCCGTTGTATCGTGCCCTGACGGACGTGGGCGCGGTGAAGGTGGTTAAAAAAGAGATGGCCCAGGGGCAAAAGCAGAGTCGCTTTATTGCCGGGACCATTAATAacgac                                                                                                                             >  2:4901657/1‑150 (MQ=255)
                         aTGTGGTTTACATCACTGGTATCTCGTGGTGAAAACTTACCGCCGTTGTATCGTGCCCTGACGGACGTGGGCGCGGTGAAGGTGGTTAAAAAAGAGATGGCCCAGGGGCAAAAGCAGAGTCGCGTTATTTCCAGGACCAAGATGAacgac                                                                                                                             >  2:3313443/1‑150 (MQ=255)
                           gtgGTTTACATCACTGGTATCTCGTGGTGAAAACTTACCGCCGTTGTATCGTGCCCTGACGGACGTGGGCGCGGTGAAGGTGGTTAAAAAAGAGATGGCCCAGGGGCAAAAGCAGAGTCGCTTTATTGCCTGGACCTTTATGAAcgacga                                                                                                                           <  2:3853307/150‑1 (MQ=255)
                               tttACATCACTGGTATCTCGTGGTGAAAACTTACCGCCGTTGTATCGTGCCCTGACGGACGTGGGCGCGGTGAAGGTGGTTAAAAAAGAGATGGCCCAGGGGCAAAAGCAGAGTCGCTTTATTGCCTGGACCTTTATGAACGACGagcag                                                                                                                       <  1:8736620/150‑1 (MQ=255)
                                      cACTGGTATCTCGTGGTGAAAACTTACCGCCGTTGTATGGTGCCCTGACGGACGTGGGCGCGGTGAAGGTGGTTAAAAAAGAGATGGCCCAGGGGCAAAAGCAGAGTCGCTTTATTGCCTGGACCTTTATGAACGACGAGCAGCgccgcc                                                                                                                <  1:11916733/150‑1 (MQ=255)
                                      cACTGGTATCTCGTGGTGAAAACTTACCGCCGTTGTATCGTGCCCTGACGGACGTGGGCGCGGTGAAGGTGGTTAAAAAAGAGATGGCCCAGGGGCAAAAGCAGAGTCGCTTTATTTCCTGGACCATTATTAAACACGAGCAGCgccccc                                                                                                                >  2:6346203/1‑150 (MQ=255)
                                       aCTGGTATCTCGTGGTGAAAACTTACCGCCGTTGTATCGTGCCCTGACGGACGTGGGCGCGGTGAAGGTGGTTAAAAAAGAGATGGCCCAGGGGCAAAAGCAGAGTCGCTTTATTGCCTGGACCGTTATGAACGACGGGCAGCGccgccg                                                                                                               >  1:988627/1‑150 (MQ=255)
                                         tGGTATCTCGTGGTGAAAACTTACCGCCGTTGTATCGTGCCCTGACGGACGTGGGCGCGGTGAAGGTGGTTAAAAAAGAGATGGCCCAGGGGCAAAAGCAGAGTCGCTTTATTGCCGGGACCTTTATGAAAGACGAGCAGCGCCGCCGtt                                                                                                             >  2:3072155/1‑150 (MQ=255)
                                           gTATCTCGTGGTGAAAACTTACCGCCGTTGTATCGTGCCCTGACGGACGTGGGCGCGGTGAAGGTGGTTAAAAAAGAGATGGCCCAGGGGCAAAAGCAGAGTCGCTTTATTGCCTGGACCTTTATGAACGACGAGCAGCGCCGCCGtttt                                                                                                           <  2:8077549/150‑1 (MQ=255)
                                             aTCTCGTGGTGAAAACTTACCGCCGTTGTATCGTGCCCTGACGGACGTGGGCGCGGTGAAGGTGGTTAAAAAAGAGATGGCCCAGGGGCAAAAGCAGAGTCGCTTTATTGCCTGGACCTTTATGAACGACGAGCAGCGCCGCCGTTTTGt                                                                                                         <  1:2317321/150‑1 (MQ=255)
                                             aTCTCGTGGTGAAAACTTACCGCCGTTGTATCGTGCCCTGACGGACGTGGGCGCGGTGAAGGTGGTTAAAAAAGAGATGGCCCAGGGGCAAAAGCAGAGTCGCTTTATTGCCTGGACCTTTATGAACGACGAGCAGCGCCGCCGTTTTGt                                                                                                         <  1:8967982/150‑1 (MQ=255)
                                                  gtggtgAAAACTTACCGCCGTTGTATCGTGCCCTGACGGACGTGGGCGCGGTGAAGGTGGTTAAAAAAGAGATGGCCCAGGGGCAAAAGCAGAGTCGCTTTATTGCCTGGACCTTTATGAACGACGAGCAGCGCCGCCGTTTTGTCAATc                                                                                                    <  1:4046348/150‑1 (MQ=255)
                                                  gtggtgAAAACTTACCGCCGTTGTATCGTGCCCTGACGGACGTGGGCGCGGTGAAGGTGGTTAAAAAAGAGATGGCCCAGGGGCAAAAGCAGAGTCGCGTTATTGCCTGGACCTTTATGAACGACGAGCAGCGCCGCCGTTTTGTCAATc                                                                                                    <  2:4305215/150‑1 (MQ=255)
                                                      tgAAAACTTACCGCCGTTGTATCGTGCCCTGACGGACGTGGGCGCGGTGAAGGTGGTTAAAAAAGAGATGGCCCAGGGGCAAAAGCAGAGTCGCTTTATTGCCTGGACCTTTATGAACGACGAGCAGCGCCGCCGTTTTGTCAATCGCCa                                                                                                <  1:5640862/150‑1 (MQ=255)
                                                        aaaaCTTACCGCCGTTGTATCGTGCCCTGACGGACGTGGGCGCGGTGAAGGTGGTTAAAAAAGAGATGGCCCAGGGGCAAAAGCAGAGTCGCTTTATTGCCTGGACCTTTATGAACGACGAGCAGCGCCGCCGTTTTGTCAATCGCCAGc                                                                                              <  1:3746951/150‑1 (MQ=255)
                                                        aaaaCTTACCGCCGTTGTATCGTGCCCTGACGGACGTGGGCGCGGTGAAGGTGGTTAAAAAAGAGATGGCCCAGGGGCAAAAGCAGAGTCGCGTTATTGCCTGGACCATTATTAAACACGAGCAGCGCCCCCCGTTTTTCAAAAGCCacc                                                                                              >  2:2477081/1‑148 (MQ=255)
                                                            cTTACCGCCGTTGTATCGTGCCCTGACGGACGTGGGCGCGGTGAAGGTGGTTAAAAAAGAGATGGCCCAGGGGCAAAAGCAGAGTCGCGTTATTGCCAGGGCCCTTAATAAAAAAGAGCAGCGCCCCCCGTTTGTAAATAGCCACCGTTa                                                                                          >  1:7660516/1‑150 (MQ=255)
                                                             ttACCGCCGTTGTATCGTGCCCTGACGGACGTGGGCGCGGTGAAGGTGGTTAAAAAAGAGATGGCCCAGGGGCAAAAGCAGAGTCGCTTTTTTTGCTGGACCTTTTTTAAAGAAGAGCAGCGCCGCCGTTTTTTCAATAGCCAGCGTTaa                                                                                         >  2:1731462/1‑150 (MQ=255)
                                                             ttACCGCCGTTGTATCGTGCCCTGACGGACGTGGGCGCGGTGAAGGTGGTTAAAAAAGAGATGGCCCAGGGGCAAAAGCAGAGTCGCTTTATTGCCTGGACCATTATGAACGACGGGCAGCGCCGCCGTTTTGTCAATAGCCAGCGTTaa                                                                                         >  2:4140615/1‑150 (MQ=255)
                                                                  gccgTTGTATCGTGCCCTGACGGACGTGGGCGCGGTGAAGGTGGTTAAAAAAGAGATGGCCCAGGGGCAAAAGCAGAGTCGCTTTATTGCCTGGACCTTTATGAACGACGAGCAGCGCCGCCGTTTTGTCAATCGCCAGCGTTAAAGCGt                                                                                    <  2:3195640/150‑1 (MQ=255)
                                                                   ccgTTGTATCGTGCCCTGACGGACGTGGGCGCGGTGAAGGTGGTTAAAAAAGAGATGGCCCAGGGGCAAAAGCAGAGTCGCTTTATTGCCTGGACCTTTATGAACGACGAGCAGCGCCGCCGTTTTGTCAATCGCCAGCGTTAAAGCGtt                                                                                   <  2:7835800/150‑1 (MQ=255)
                                                                   ccgTTGTATCGTGCCCTGACGGACGTGGGCGCGGTGAAGGTGGTTAAAAAAGAGATGGCCCAGGGGCAAAAGCAGAGTCGCTTTATTGCCTGGACCTTTATGAACGACGAGCAGCGCCGCCGTTTTGTCAATCGCCAGCGTTAAAGCGtt                                                                                   <  1:10217341/150‑1 (MQ=255)
                                                                   ccgTTGTATCGTGCCCTGACGGACGTGGGCGCGGTGAAGGTGGTTAAAAAAGAGATGGCCCAGGGGCAAAAGCAGAGTCGCTTTATTGCCTGGACCTTTATGAACGACGAGCAGCGCCGCCGTTTTGTCAATCGCCAGCGTTAAAGCGtt                                                                                   <  1:8389581/150‑1 (MQ=255)
                                                                   ccgTTGTATCGTGCCCTGACGGACGTGGGCGCGGTGAAGGTGGTTAAAAAAGAGATGGCCCAGGGGCAAAAGCAGAGTCGCTTTATTGCCTGGACCTTTATGAACGACGAGCAGCGCCGCCGTTTTGTCAATCGCCAGCGTTAAAGCGtt                                                                                   <  1:8502119/150‑1 (MQ=255)
                                                                   ccgTTGTATCGTGCCCTGACGGACGTGGGCGCGGTGAAGGTGGTTAAAAAAGAGATGGCCCAGGGGCAAAAGCAGAGTCGCTTTATTGCCTGGACCTTTATGAACGACGAGCAGCGCCGCCGTTTTGTCAATCGCCAGCGTTAAAGCGtt                                                                                   <  1:243392/150‑1 (MQ=255)
                                                                    cgTTGTATCGTGCCCTGACGGACGTGGGCGCGGTGAAGGTGGTTAAAAAAGAGATGGCCCAGGGGCAAAAGCAGAGTCGCTTTATTGCCTGGACCTTTATGAACGACGAGCAGCGCCGCCGTTTTGTCAATCGCCAGCGTTAAAGCGTTg                                                                                  <  2:12057004/150‑1 (MQ=255)
                                                                        gTATCGTGCCCTGACGGACGTGGGCGCGGTGAAGGTGGTTAAAAAAGAGATGGCCCAGGGGCAAAAGCAGAGTCGCTTTATTGCCTGGACCTTTATGAACGACGAGCAGCGCCGCCGTTTTGTCAATCGCCAGCGTTAAAGCGTTGGTTc                                                                              <  1:1456143/150‑1 (MQ=255)
                                                                             gTGCCCTGACGGACGTGGGCGCGGTGAAGGTGGTTAAAAAAGAGATGGCCCAGGGGCAAAAGCAGAGTCGCTTTATTGCCAGGCCCATTATGAACCACGAGCAGCGCCCCCGGTTTGTCAATAGCCACCGTTAAAGCCTTGGTTcccccc                                                                         >  2:4660673/1‑149 (MQ=255)
                                                                             gTGCCCTGACGGACGTGGGCGCGGTGAAGGTGGTTAAAAAAGAGATGGCCCAGGGGCAAAAGCAGAGTCGCGTTATTTGCTGGACCGTTATTAACGACGAGCAGCCCCCCCCTTTTGTCAAACGCCCGCCGTGAATCGTTGGTTaccccc                                                                         >  1:5431106/1‑149 (MQ=255)
                                                                               gCCCTGACGGACGTGGGCGCGGTGAAGGTGGTTAAAAAAGAGATGGCCCAGGGGCAAAAGCAGAGTCGCTTTATTGCCTGGACCTTTATGAACGACGAGCAGCGCCGCCGTTTTGTCAATCGCCAGCGTTAAAGCGTTGGTTCCCCcggc                                                                       <  1:5267711/150‑1 (MQ=255)
                                                                                cccTGACGGACGTGGGCGCGGTGAAGGTGGTTAAAAAAGAGATGGCCCAGGGGCAAAAGCAGAGTCGCTTTATTGCCGGGACCTTTATGAACGACGAGCAGCGCCGCCGGTTTGTCAATCGCCAGCGTTAAAGCGTTGGTTCCCCcggcg                                                                      >  2:6981642/1‑150 (MQ=255)
                                                                                cccTGACGGACGTGGGCGCGGTGAAGGTGGTTAAAAAGAGATGGCCCAGGGGCAAAAGCAGAGTCGCTTTTTTTCCTGGACCTTTTTTTAAGACCAGCAGCCCCCCCGGTTTTTCAATAGCCACCCGTAAATCGTTGGTTACCCccgccg                                                                     >  1:10706499/1‑148 (MQ=255)
                                                                                  cTGACGGACGTGGGCGCGGTGAAGGTGGTTAAAAAAGAGATGGCCCAGGGGCAAAAGCAGAGTCGCGTTATTTGCTGGACCGTTATTAAAGACGAGCAGCGCCGCCGTTTTTTAAATAGCCAGCGGTAAAGCGGTTGTTGCCCccgcggg                                                                    >  1:6298024/1‑149 (MQ=255)
                                                                                    gacggacgTGGGCGCGGTGAAGGTGGTTAAAAAAGAGTTGGCCCAGGGGCAAAAGCAGAGTCGCTTTATTGCCTGGACCTTTATGAACGACGAGCAGCGCCGCCGTTTTGTCAATCGCCAGCGTTAAAGCGTTGGTTCCCCCGGCGGTaa                                                                  <  2:3610988/150‑1 (MQ=255)
                                                                                      cggacgTGGGCGCGGTGAAGGTGGTTAAAAAAGAGATGGCCCAGGGGCAAAAGCAGAGTCGCTTTATTGCCTGGACCTTTATGAACGACGAGCAGCGCCGCCGTTTTGTCAATCGCCAGCGTTAAAGCGTTGGTTCCCCCGGCGGTAACg                                                                <  1:817978/150‑1 (MQ=255)
                                                                                      cggacgTGGGCGCGGTGAAGGTGGTTAAAAAAGAGATGGCCCAGGGGCAAAAGCAGAGTCGCTTTATTGCCTGGACCTTTATGAACGACGAGCAGCGCCGCCGTTTTGTCAATCGCCAGCGTTAAAGCGTTGGTTCCCCCGGCGGTAACg                                                                <  2:2210662/150‑1 (MQ=255)
                                                                                       ggacgTGGGCGCGGTGAAGGTGGTTAAAAAAGAGATGGCCCAGGGGCAAAAGCAGAGTCGCTTTATTGCCTGGACCTTTATGCACGACGAGCAGCGCCGCCGTTTTGTCAATCGCCAGCGTTAAAGCGTTGGTTCCCCCGGCGGTAACgg                                                               <  1:4799858/150‑1 (MQ=255)
                                                                                        gacgTGGGCGCGGTGAAGGTGGTTAAAAAAGAGATGGCCCAGGGGCAAAAGCAGAGTCGCTTTATTGCCTGGACCTTTATGAACGACGAGCAGCGCCGCCGTTTTGTCAATCGCCAGCGTTAAAGCGTTGGTTCCCCCGGCGGTAACGGt                                                              <  1:6828663/150‑1 (MQ=255)
                                                                                           gTGGGCGCGGTGAAGGTGGTTAAAAAAGAGATGGCCCAGGGGCAAAAGCAGAGTCGCGTTATTGCCTGGACCGTTAATAACGACGAGCAGCGCCCCCGGTTTGTCAATCGCCCGCCGTAAAACGTTTGTTCCCCCCGCGGTAACCGGTCa                                                           >  2:6516157/1‑150 (MQ=255)
                                                                                               gcgcGGTGAAGGTGGTTAAAAAAGAGATGGCCCAGGGGCAAAAGCAGAGTCGCGTTATTTCCGGGGCCATTATTAAAGACGAGCAGCCCCCCCCTTTTGTCAAACGCCACCCTTAAAACGGTGGTTCCCCCCGCCGTAACCGTTCAGccc                                                       >  1:5069163/1‑149 (MQ=255)
                                                                                                 gcgATGAAGGTGGTTAAAAAAGAGATGGCCCAGGGGCAAAAGCAGAGTCGCTTTATTGCCTGGACCTTTATGAACGACGAGCAGCGCCGCCGTTTTGTCAATCGCCAGCGTTAAAGCGTTGGTTCCCCCGGCGGTAACGGTTCAGCCggg                                                     <  2:8404891/150‑1 (MQ=255)
                                                                                                   ggTGAAGGTGGTTAAAAAAGAGATGGCCCAGGGGCAAAAGCAGAGTCGCTGTATTGCCTGGACCAATATTAACGACGAGCAGACCCCCCCTTTTTTCAAACGCCCGCGGTAAAACGTTTGTTCCCCCCGCGGTTACGGTTCAGCCCGGGt                                                   >  2:10480662/1‑150 (MQ=255)
                                                                                                     tGAAGGTGGTTAAAAAAGAGATGGCCCAGGGGCAAAAGCAGAGTCGCTTTAATGCCAGGACCATTATTAAAGACGAGCAGCCCCGCCCGTTTGTCAATCGCCCGCGTTAAAGCCTTTGGTACCCCCGCGGTAACCGGTCAGCCCGGGTcg                                                 >  2:10952237/1‑150 (MQ=255)
                                                                                                       aaGGTGGTTAAAAAAGAGATGGCCCAGGGGCAAAAGCAGAGTCGCTTTATTGCCGGGACCATTATTAACCACGCGCAGCGCCGCCGTTTTGTAAAACGCCACCGTTAAATCGGTGGTTCCCCCCGCGGTAAAGGTTCAGCCcggggagcg                                               >  2:2583347/1‑145 (MQ=255)
                                                                                                          gtggtTAAAAAAGAGTTGGCCCAGGGGCAAAAGCAGAGTCGCTTTATTGCCTGGACCTTTATGAACGACGAGCAGCGCCGCCGTTTTGTCAATCGCCAGCGTTAAAGCGTTGGTTCCCCCGGCGGTAACGGTTCAGCCGGGGTCGCGccc                                            <  1:7893667/150‑1 (MQ=255)
                                                                                                          gtggtTAAAAAAGAGATGGCCCAGGGGCAAAAGCAGAGTCGCTTTATTGCCTGGACCTTTATGAACGACGAGCAGCGCCGCCGTTTTGTCAATCGCCAGCGTTAAAGCGTTGGTTCCCCCGGCGGTAACGGTTCAGCCGGGGTCGCGccc                                            <  2:9869993/150‑1 (MQ=255)
                                                                                                          gtggtTAAAAAAGAGACGGCCCAGGGGCAAAAGCAGAGTCGCTTTATTGCCTGGACCTTTATGAACGACGAGCAGCGCCGCCGTTTTGTCAATCGCCAGCGTTAAAGCGTTGGTTCCCCCGGCGGTAACGGTTCAGCCGGGGTCGCGccc                                            <  1:11514870/150‑1 (MQ=255)
                                                                                                           tggtTAAAAAAGAGATGGCCCAGGGGCAAAAGCAGAGTCGCTTTATTGCCTGGACCTTTATGAACGACGAGCAGCGCCGCCTTTTTGTCAATCGCCAGCGTTAAAGCGTTGGTTCCCCCGGCGGTAACGGTTCAGCCGGGGTCGCGCCCg                                           <  1:3177081/150‑1 (MQ=255)
                                                                                                           tggtTAAAAAAGAGATGGCCCAGGGGCAAAAGCAGAGTCGCTTTATTGCCTGGACCTTTATGAACGACGAGCAGCGCCGCCGTTTTGTCAATCGCCAGCGTTAAAGCGTTGGTTCCCCCGGCGGTAACGGTTCAGCCGGGGTCGCGCCCg                                           <  2:9585995/150‑1 (MQ=255)
                                                                                                           tggtTAAAAAAGAGATGGCCCAGGGGCAAAAGCAGAGTCGCTTTATTGCCTGGACCTTTATGAACGACGAGCAGCGCCGCCGTTTTGTCAATCGCCAGCGTTAAAGCGTTGGTTCCCCCGGCGGTAACGGTTCAGCCGGGGTCGCGCCCg                                           <  2:7208634/150‑1 (MQ=255)
                                                                                                           tggtTAAAAAAGAGATGGCCCAGGGGCAAAAGCAGAGTCGCTTTATTGCCTGGACCTTTATGAACGACGAGCAGCGCCGCCGTTTTGTCAATCGCCAGCGTTAAAGCGTTGGTTCCCCCGGCGGTAACGGTTCAGCCGGGGTCGCGCCCg                                           <  2:10790857/150‑1 (MQ=255)
                                                                                                              ttAAAAAAGAGATGGCCCAGGGGCAAAAGCAGAGTCGCGTTATTTGCTGGACCATTATTAAAGACGAGCAGCGCCGCCCGTTTTTAAAAAGCCACCGTTAAATCGTTGGTTCCCCCCGCCGGAAAGGTTCACCCGGGGTCGCGcccccag                                        >  2:1117964/1‑146 (MQ=255)
                                                                                                               tAAAAAAGAGATGGCCCAGGGGCAAAAGCAGAGTCGCTTTATTGCCTGGACCTTTATGAACGACGAGCAGCGCCGCCGTTTTGTCAATCGCCAGCGTTAAAGCGTTGGTTCCCCCGGCGGTAACGGTTCAGCCGGGGTCGCGCCCGGAgc                                       <  1:6010455/150‑1 (MQ=255)
                                                                                                                aaaaaaGAGATGGCCCAGGGGCAAAAGCAGAGTCGCTTTATTGCCTGGACCTTTATGAACGACGAGCAGCGCCGCCGTTTTGTCAATCGCCAGCGTTAAAGCGTTGGTTCCCCCGGCGGTAACGGTTCAGCCGGGGTCGCGCCCGGAgca                                      <  2:6170116/150‑1 (MQ=255)
                                                                                                                 aaaaaGAGATGGCCCAGGGGCAAAAGCAGAGTCGCGTTATTGCCGGGCCCGTTATGAACCCCGAGCCGCGCCCCCCGGTTGTCAAACGCCCGCGGGTAAGCCGTGGGTCCCCCCGCCGGGACGGGTCAGCCCGGGGCGCGccccgcgccg                                     >  2:4997501/1‑143 (MQ=255)
                                                                                                                  aacagagATGGCCCCGGGGCAAACGCAGAGTCGCTTTAGTGCCTGGACCTTTATGACCGACGAGCAGCGCCGCCGTTTTGTCAATCGCCAGCGTTAAAGCGTTGGTTCCCCCGGCGGTTTCGGTTCAGCCGGGGTCGCGCCCGGAgcagg                                    <  2:8744908/147‑1 (MQ=255)
                                                                                                                    aaGAGATGGCCCAGGGGCAAAAGCAGAGTCGCTTTATTGCCTGGACCTTTATGAACGACGAGCAGCGCCGCCGTTTTGTCAATCGCCAGCGTTAAAGCGTTGGTTCCCCCGGCGGTAACGGTTCAGCCGGGGTCGCGCCCGGAgcaggca                                  <  2:1387497/150‑1 (MQ=255)
                                                                                                                    aaGAGATGGCCCAGGGGCAAAAGCAGAGTCGCGTTATTGCCTGGACCTTTATGAACGACGAGCAGCGCCGCCGTTTTGTCAATCGCCAGCGTTAAAGCGTTGGTTCCCCCGGCGGTAACGGTTCAGCCGGGGTCGCGCCCGGAgcaggca                                  <  2:5569946/150‑1 (MQ=255)
                                                                                                                       agaTGGCCCAGGGGCAAAAGCAGAGTCGCTTTATTGCCTGGACCTTTATGAACGACGAGCAGCGCCGCCGTTTTGTCAATCGCCAGCGTTAAAGCGTTGGTTCCCCCGGCGGTAACGGTTCAGCCGGGGTCGCGCCCGGAGCAGgcagca                               <  1:11726314/150‑1 (MQ=255)
                                                                                                                           ggCCCAGGGGCAAAAGCAGAGTCGCTTTATTGCCTGGACCTTTATGAACGACGAGCAGCGCCGCCGTTTTGTCAAACGCCAGCGTTAAAGCGTTGGTTCCCCCGGCGGTAACCGTTCAGCCGGGGTCGCGCCCGGAGCAGGCAGCACCTg                           >  2:9062699/1‑150 (MQ=255)
                                                                                                                            gCCCAGGGGCAAAAGCAGAGTCGCTTTATTGCCTGGACCTTTATGAACGACGAGCAGCGCCGCCGTTTTGTCAATCGCCAGCGTTAAAGCGTTGGTTCCCCCGGCGGTAACGGTTCAGCCGGGGTCGCGCCCGGAGCAGGCAGCACCTGa                          <  1:5794310/150‑1 (MQ=255)
                                                                                                                            gCCCAGGGGCAAAAGCAGAGTCGCTTTATTGCCTGGACCTTTATGAACGACGAGCAGCGCCGCCGTTTTGTCAATCGCCAGCGTTAAAGCGTTGGTTCCCCCGGCGGTAACGGTTCAGCCGGGGTCGCGCCCGGAGCAGGCAGCACCTGa                          <  1:6088540/150‑1 (MQ=255)
                                                                                                                            gCCCAGGGGCAAAAGCAGAGTCGCTTTATTGCCTGGACCTTTATGAACGACGAGCAGCGCCGCCGTTTTGTCAATCGCCAGCGTTAAAGCGTTGGTTCCCCCGGCGGTAACGGTTCAGCCGGGGTCGCGCCCGGAGCAGGCAGCACCTGa                          <  2:608142/150‑1 (MQ=255)
                                                                                                                              ccAGGGGCAAAAGCAGAGTCGCTTTATTGCCTGGACCATTATGAACGACGAGCCGCGCCGCCCTTTTGTCAATAGCCAGCGGTAAAGCGTTGGTTCCCCCGGCGGTAACGGTTCAGCCCGGGTCGCGCCCGGAGCAGGCAGCACCTGATa                        >  2:5176794/1‑150 (MQ=255)
                                                                                                                               ccggggCAAAAGCAGAGTCGCTTTATTGCCTGGACCTTTATGAACGACGAGCAGCGCCGCCGTTTTGCCAATCGCCAGCGTTAAAGCGTTGGTTCCCCCGGCGGTAACGGTTCAGCCGGGGTCGCGCCCGGAGCAGGCAGCACCTGATaa                       <  2:9159704/148‑1 (MQ=255)
                                                                                                                               cAGGGGCAAAAGCAGAGTCGCTTTATTGCCTGGACCTTTATGAACGACGAGCAGCGCCGCCGTTTTGTCAATCGCCAGCGTTAAAGCGTTGGTTCCCCCGGCGGTAACGGTTCAGCCGGGGTCGCGCCCGGAGCAGGCAGCACCTGATaa                       <  1:6550006/150‑1 (MQ=255)
                                                                                                                               cAGGGGCAAAAGCAGAGTCGCGTTATTGCCTGGACCGTTATGAACGGCGAGCCGCGCCGCCGGTTTGTCCATCGCCAGCCGTTAAGCGGTGGTTCCCCCGGCGGGAACCGGTAAGCCCGGGTCGCGCCCCGCGCAGGCAGCACCGGATaa                       >  2:5698457/1‑150 (MQ=255)
                                                                                                                                aGGGGCAAAAGCAGAGTCGCGTTTTTGCCGGGGCCCTTATGAACGACGCGCAGCGCCCCCCGTTTGTCAAACCCCCCCCGTAAAGCCGTGGGTCCCCCCGCGGGAACCGGTCACCCCGGGGCGCGCCCCGCGCACGCCCCCCCAGATAAg                      >  2:3546339/1‑150 (MQ=255)
                                                                                                                                  gggCAAAAGCAGAGTCGCTTTATTTCCGGGACCGTTATGAACGACGAGCAGCGCCCCCCTTTTGTCAATAGCCAGCGTTAAAGCGTTGGTTACCCCCGCGGTAACGGTTCAGCCCGGGTCGCGCCCGGAGCAGGCAGCACCAGATAAGTc                    >  2:1015062/1‑150 (MQ=255)
                                                                                                                                   ggCAAAAGCAGAGTCGCTTTATTGCCTGGACCTTTATGAACGACGAGCAGCGCCGCCGTTTTGTCAATCGCCAGCGTTAAAGCGTTGGTTCCCCCGGCGGTAACGGTTCAGTCGGGGTCGCGCCCGGAGCAGGCAGCACCTGATAAGTCt                   <  2:4761597/150‑1 (MQ=255)
                                                                                                                                         aGCAGAGTCGCTTTATTGCCTGGACCTTTATGAACGACGAGCAGCGCCGCCGTTTTGTCAATCGCCAGCGTTAAAGCGTTGGTTCCCCCGGCGGTAACGGTTCAGCCGGGGTCGCGCCCGGAGCAGGCAGCACCTGATAAGTCTGCACTg             <  1:10069903/150‑1 (MQ=255)
                                                                                                                                          gCAGAGTCGCTTTATTGCCAGGACCATTATTAACGAAGAGCAGCGCCGCCGTTTTTTCAATAGCCAGCGTTAAAGCGTTGGTTCCCCCGGCGGTAACGGTTCAGCCGGGGTCGCGCCCGGAGCAGGCAGCACCAGATAAGTCTGCACAgg            >  2:11250188/1‑150 (MQ=255)
                                                                                                                                           cAGAGTCGCTTTATTGCCTGGACCTTTATGAACGACGAGCAGCGCCGCCGTTTTGTCAATCGCCAGCGTTAAAGCGTTGGTTCCCCCGGCGGTAACGGTTCAGCCGGGGTCGCGCCCGGAGCAGGCAGCACCTGATAAGTCTGCACTGgc           <  1:111945/150‑1 (MQ=255)
                                                                                                                                           cAGAGTCGCTTTATTGCCTGGACCTTTATGAACGACGAGCAGCGCCGCCGTTTTGTCAATCGCCAGCGTTAAAGCGTTGGTTCCCCCGGCGGTAACGGTTCAGCCGGGGTCGCGCCCGGAGCAGGCAGCACCTGATAAGTCTGCACTGgc           <  1:1971422/150‑1 (MQ=255)
                                                                                                                                                tCGCTTTATTGCCTGGACCTTTATGAACGACGAGCAGCGCCGCCGTTTTGTCAATCGCCAGCGTTAAAGCGTTGGTTCCCCCGGCGGTAACGGTTCAGCCGGGGTCGCGCCCGGAGCAGGCAGCACCTGAGACGTCTGCACTGGcgcgcg      >  2:6402970/1‑150 (MQ=255)
                                                                                                                                                   cTTTATTGCCTGGACCTTTATGAACGACGAGCAGCGCCGCCGTTTTGTCAATCGCCAGCGTTAAAGCGTTGGTTCCCCCGGCGGTAACGGTTCAGCCGGGGTCGCGCCCGGAGCAGGCAGCACCTGATAAGTCTGCACTGGCGCGCGAAc   <  1:7403774/150‑1 (MQ=255)
                                                                                                                                                   cTTTATTGCCTGGACCTTTATGAACGACGAGCAGCGCCGCCGTTTTGTCAATCGCCAGCGTTAAAGCGTTGGTTCCCCCGGCGGTAACGGTTCAGCCGGGGTCGCGCCCGGAGCAGGCAGCACCTGATAAGTCTGCACTGGCGCGCGAAc   >  2:276260/1‑150 (MQ=255)
                                                                                                                                                   cTTTATTGCCTGGACCTTTATGAACGACGAGCAGCGCCGCCGTTTTGTCAATCGCCAGCGTTAAAGCGTTGGTTCCCCCGGCGGTAACGGTCCAGCCGGGGTCGCGCCCGGAGCAGGCAGCACCTGATAAGTCTGCACTGGCGCGCGAAc   <  1:9547977/150‑1 (MQ=255)
                                                                                                                                                   cTTTAGTGCCTGGACCTTTATGACCGACGAGCAGCGCCGCCGTTTTGTCAATCGCCAGCGTTAAAGCGTTGGTTCCCCCGGCGGTAACGGTTCAGCCGGGGTCGCGCCCGGAGCAGGCAGCACCTGATAAGTCTGCACTGGCGCGCGAAc   <  2:7265062/150‑1 (MQ=255)
                                                                                                                                                    tttATTGCCTGGACCTTTATGAACGACGAGCAGCGCCGCCGTTTTGTCAATCGCCAGCGTTAAAGCGTTGGTTCCCCCGGCGGTAACGGTTCAGCCGGGGTCGCGCCCGGAGCAGGCAGCACCTGATAAGTCTGCACTGGCGCGCGAAcg  >  1:6718276/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
GAGCAAAGGCTTCGCGAAGCAGGTGATGTGGTTTACATCACTGGTATCTCGTGGTGAAAACTTACCGCCGTTGTATCGTGCCCTGACGGACGTGGGCGCGGTGAAGGTGGTTAAAAAAGAGATGGCCCAGGGGCAAAAGCAGAGTCGCTTTATTGCCTGGACCTTTATGAACGACGAGCAGCGCCGCCGTTTTGTCAATCGCCAGCGTTAAAGCGTTGGTTCCCCCGGCGGTAACGGTTCAGCCGGGGTCGCGCCCGGAGCAGGCAGCACCTGATAAGTCTGCACTGGCGCGCGAACG  >  NC_000913/843048‑843345

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: