Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 915,215 T→G 10.0% intergenic (‑358/+137) ybjE ← / ← aqpZ putative transporter/aquaporin Z

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913915,2150TG10.0% 1098.7 / 26.6 431intergenic (‑358/+137)ybjE/aqpZputative transporter/aquaporin Z
Reads supporting (aligned to +/- strand):  ref base T (298/78);  new base G (5/50);  total (303/128)
Fisher's exact test for biased strand distribution p-value = 1.40e-24
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 437 total aligned reads displayed.

GGGGCAGCCCACATGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTAAAAAAAGGCCTGACATTACGCCAGGCCTTCTGCGTTAATTAATCACGCTTT  >  NC_000913/915066‑915364
                                                                                                                                                       |                                                                                                                                                     
 gggCAGCCCACCTGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTg                                                                                                                                                      <  1:10852953/150‑1 (MQ=17)
 gggCAGCCCACATGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTg                                                                                                                                                      <  2:7343535/150‑1 (MQ=32)
  ggCAGCCCACATGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTgg                                                                                                                                                     <  2:7783503/150‑1 (MQ=32)
  ctcAGCCCACATGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCAGACACGGTAGCCGTACGGGATAAGTCGAGGGCACCGCGCAGCGGCGATGTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGAGTgg                                                                                                                                                     >  2:7992634/3‑150 (MQ=11)
  ctcAGCCCACATCCATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTgg                                                                                                                                                     >  2:4133691/3‑150 (MQ=12)
    cAGCCCACATGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGgcc                                                                                                                                                   <  2:6422956/150‑1 (MQ=32)
    cAGCCCACATGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCAAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGgcc                                                                                                                                                   <  2:2385876/150‑1 (MQ=17)
      gCCCACATGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGgccgc                                                                                                                                                 <  2:8682499/150‑1 (MQ=32)
       cccACATGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGgccgcc                                                                                                                                                >  2:8565019/1‑150 (MQ=32)
             tGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCTgc                                                                                                                                          >  1:4970338/1‑150 (MQ=32)
              ccaTGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCTgcg                                                                                                                                         >  2:2197188/3‑150 (MQ=17)
                    gggCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCTGCGCGCTcc                                                                                                                                   >  2:3176095/1‑150 (MQ=32)
                     ggCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCTGCGCGCTCCt                                                                                                                                  >  2:1035562/1‑150 (MQ=32)
                        tGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCTGCGCGCTCCTTgc                                                                                                                               >  1:2543668/1‑150 (MQ=32)
                        tGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCTGCGCGCTCCTTgc                                                                                                                               >  2:3572680/1‑150 (MQ=32)
                          aGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCTGCGCGCTCCTTgcgc                                                                                                                             >  1:9378737/1‑150 (MQ=32)
                             gcgcGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCTGCGCGCTCCTTGCGCCAg                                                                                                                          >  2:840169/1‑150 (MQ=32)
                             gcgcGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCAGCGCGCTCCTTGCGCCAg                                                                                                                          >  1:9751261/1‑150 (MQ=17)
                                 gTTTTACTGGGATGTTACCCCGCGCCCGACCGGGTTGCCTTACGGGATAAGTCGCGGGCACCGCGCCGCGGCGTTTTTGTTCGCCAGAGCCGGGGGGGGCAGGGGGCGGCGGCTATTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGc                                                                                                                      <  2:9036374/150‑1 (MQ=11)
                                 gTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGGTTTGCCCCCCCCGGCGCGCACCATGCGCCCGTGGc                                                                                                                      >  1:3086227/1‑150 (MQ=11)
                                 gTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGc                                                                                                                      >  2:10535832/1‑150 (MQ=33)
                                 gTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATGGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGc                                                                                                                      >  1:10624936/1‑150 (MQ=18)
                                  ttttACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCa                                                                                                                     >  2:3013087/1‑150 (MQ=34)
                                      aCAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAatat                                                                                                                 >  2:8212480/1‑150 (MQ=38)
                                        aGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGt                                                                                                               >  1:2796393/1‑150 (MQ=39)
                                        aGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGt                                                                                                               >  2:10805126/1‑150 (MQ=39)
                                        aGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGt                                                                                                               >  1:1268171/1‑150 (MQ=39)
                                        aGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGt                                                                                                               >  2:11787845/1‑150 (MQ=39)
                                        aGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCAGCGCGCTCCTTGCGCCAGTGGCAATATGt                                                                                                               >  1:7556159/1‑150 (MQ=255)
                                        aGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGAGTGGCCGCCCCCGGCGCGCTCCATGCGCCAGTGGCCATATGt                                                                                                               >  2:9270628/1‑150 (MQ=255)
                                          ggATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTg                                                                                                             >  1:6818761/1‑150 (MQ=40)
                                          ggATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTg                                                                                                             >  1:7842376/1‑150 (MQ=40)
                                          ggATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCAGCGCGCTCCTTGCGCCAGTGGCAATATGTTg                                                                                                             >  1:358070/1‑150 (MQ=255)
                                           gATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGc                                                                                                            >  2:10259160/1‑150 (MQ=255)
                                                tACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCTGCGCGCTCCTTGCGCCCGTGGCAATATGTTGCTTAGc                                                                                                       >  2:1222005/1‑150 (MQ=255)
                                                tACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCGGCGCGCGCCTTGCGCCAGTGGCAATATGTTGCTTAGc                                                                                                       >  1:7371748/1‑150 (MQ=255)
                                                 aCCTCGCGCCCGACCGGGTAGCCGTATGGTATAAGTGGAGGGCACCGCGCAGGGGGGTTTTTGTTCGCCTGCGCCGGGGGGTGCTGGGGGCGGCGGCGATTGGCCGCCCCCGGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCt                                                                                                      <  2:6845788/150‑1 (MQ=14)
                                                   cTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCa                                                                                                    >  2:472679/1‑150 (MQ=255)
                                                    tCGCGCCCGACCCGGTAGCGGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGTGGCGGCGGCGATTGGACGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGATCAt                                                                                                   >  2:1933761/1‑150 (MQ=255)
                                                    tCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCAGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCaa                                                                                                   >  1:9048358/1‑149 (MQ=255)
                                                    tCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGAGTGGCCGCCCCCGGCGCGCTCCTTGCGCCAGTGGCAAAATGTTGCTTAGCTCAt                                                                                                   >  1:8302040/1‑150 (MQ=255)
                                                     cgcgCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCAGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATg                                                                                                  >  2:4306104/1‑150 (MQ=255)
                                                      gcgcCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGa                                                                                                 >  2:888155/1‑150 (MQ=255)
                                                         cccGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAg                                                                                              >  2:1551714/1‑150 (MQ=255)
                                                          ccGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAgg                                                                                             >  2:11461234/1‑150 (MQ=255)
                                                          ccGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCAGCGCGCTCCATGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAgg                                                                                             >  1:3021847/1‑150 (MQ=255)
                                                            gACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAg                                                                                           >  1:9849273/1‑150 (MQ=255)
                                                             aCCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCAGCGCGCGCCGTGCGCCAGTGGCAATATGTTGCTTAGCTCATGTAAGGAgc                                                                                          >  1:4749773/1‑150 (MQ=255)
                                                               ccGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCTGCGCGCGCCGTGCGCCCGGGGCAAAATGTTGCTTAGCTCCTGAAAAGAgcgc                                                                                        >  1:4621582/1‑150 (MQ=17)
                                                               ccGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCAGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCACATGAAAGGAgcgc                                                                                        >  1:1885266/1‑150 (MQ=255)
                                                               ccGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCAGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCGTAGCTCATTAAAGGAgcgc                                                                                        >  1:10050833/1‑150 (MQ=255)
                                                               ccGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCAGCGCGCGCCGTGCGCCAGTGGCAATATGTTGCTTAGCTCATGGAAGGAgcgc                                                                                        >  1:10356055/1‑150 (MQ=255)
                                                                      ccttacgggATACGTCGCGGCCCCCGCCCCGCGCCTTTTTTTTTCGCCAGCGCGGGGGGGTGCTGGGGGGGGCGGCTTTGGGCCCCCCCCCGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAga                                                                                 <  2:7689191/144‑1 (MQ=16)
                                                                       agTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCAGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCAGGAAAGGAGCGCAACAAgat                                                                                >  2:881754/2‑150 (MQ=255)
                                                                        gTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGCTTGGCCGCCCCCAGCGCGCGCCATTCGCCCCTGGCAAAAAGTTTCTTAGCTGAAGAAAAGAGCGcaacaacaag                                                                               >  1:3212227/1‑146 (MQ=14)
                                                                         tAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAgatga                                                                              >  2:11037014/1‑150 (MQ=255)
                                                                           aGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGCTTGGCCCCCCCCCCCGCGCGCCGTGCGCCCCAGGCAAAAATTTTCTTATCAAAAGAAAGGAGCGcaacaaaaataat                                                                            >  1:4432890/1‑143 (MQ=2)
                                                                             ggATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCCGGGGGCGGCCGCGGTTGGCCGCCCCCCGCGCGCGCCATGCGCCCCTGGCAAAATATTTCATATCTAATGTAAGGAGCCCAAAAAAATGATGaa                                                                          >  1:11508513/1‑150 (MQ=14)
                                                                              gatccgTCGAGGGCACCCCCGCGCGGCTATTTTGTTCGCATTAGCCGGGGGGGGCTGGGGGGGGCGGGCTTGGGCCGCCCCGCGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGAtgatgact                                                                         <  2:569408/145‑3 (MQ=16)
                                                                              gATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAAt                                                                         >  2:11105239/1‑150 (MQ=255)
                                                                                  cgTCGCGGGCACCGCGCCGCGGCGATTTTGTTCGCCAGAGCCGGGGGGTGCTGGGGGGGGGGGCGATTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAAc                                                                     <  2:6186670/149‑1 (MQ=18)
                                                                                    tCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACat                                                                   >  2:1705232/1‑150 (MQ=255)
                                                                                      gAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACatat                                                                 >  2:600689/1‑150 (MQ=255)
                                                                                                cgcAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCt                                                       >  2:10970142/1‑150 (MQ=255)
                                                                                                     gggcttttttGTTCTCCAGTGCCGGGGGGTGCCGGGGGGGGGGGCTTTGGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTaaaa                                                  <  2:5134214/144‑1 (MQ=25)
                                                                                                      ggcgATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTaaaaa                                                 >  2:4602143/1‑150 (MQ=255)
                                                                                                      ggcgATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCCCCCCCAGCGCGCGCCGTGCGCCCCGGGCCCAAAGTTGCTTAGCGCAGGCAAGGCGCGCAACAAGATGATGAAACAACAAAAAACAACAACAGaaaaa                                                 >  1:6511571/1‑150 (MQ=12)
                                                                                                       gggtttttGTTTCGCCCGCGCCCGGGGGTGCTGGGGGCGGCGGCGATGGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTaaaaa                                                 <  2:8125176/146‑1 (MQ=25)
                                                                                                       gcgATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTaaaaaa                                                >  2:8449976/1‑150 (MQ=255)
                                                                                                        cgATTTTGTTCGCCAGAGCCCGGGGGAGCAGGGGGCCGCGGCGATTGGCCGCCCCCTGCGCGCAACTTGCGCCAGTGGCAAAATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACAAAAAACAACAACATaaaaaaa                                               >  2:2611410/1‑150 (MQ=21)
                                                                                                         gtttttGGTCGCCAGCGCCGGGGGGGGCGGGGGGGGGGGGGGTGTGGCCCCCCCCGGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTAAAAAAAg                                              <  2:12001080/148‑1 (MQ=255)
                                                                                                          tttttGTGCGCCAGCGCCGGGGGGTCCGGGGGGGGGGGGCGATGGGCCCCCCCGTCCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTAAAAAAAgg                                             <  2:4946520/149‑1 (MQ=255)
                                                                                                           ttttGTTCGGCCAGCGGTTGGTGGTTCTGGGGGGGTGGGCGCTGGGCGGCCCCGTGCGAGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTAAAAAAAgg                                             <  2:6621331/150‑1 (MQ=255)
                                                                                                           ttttGTTCGCCAGAGCCCGGGGGTGCTGGGGGCGGCGGCGATTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTAAAAAAAGGc                                            <  2:7127899/150‑1 (MQ=255)
                                                                                                            tttGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTAAAAAAAGGcc                                           >  2:2816315/1‑150 (MQ=255)
                                                                                                               gTTCGCCTGCGCGGGGGGGTGGTGGGGGGGGGGGGTTTTGGCCGCCCCGTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTAAAAAAAGGCCTGa                                        <  2:7600273/150‑1 (MQ=37)
                                                                                                               gTTCGCCTGCGCGGGGGGGTGGTGGGGGGGGGGGCTTTTGGCCGCCCCCCGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTAAAAAAAGGCCTGa                                        <  2:10652857/150‑1 (MQ=37)
                                                                                                                 ttCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTAAAAAAAGGCCTGAc                                       >  2:1475398/1‑150 (MQ=255)
                                                                                                                  ttagCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTAATGAAAGGAGAGCAACAAGATGATGAATAAACAAATAACAACATCTTAAAAAAAGGCCTGaa                                       >  2:3810961/4‑149 (MQ=25)
                                                                                                                        agagCCCGGGGGTGCATGGGGCGGCGGCGATTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTAAAAAAAGGCCTGACATTACg                                 >  1:2265132/1‑150 (MQ=255)
                                                                                                                        agagCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAAACAACATATAACAACATCTTAAAAAAAGGCCAGACATTACg                                 >  2:9589610/1‑150 (MQ=255)
                                                                                                                            cggggggggGCGGGGGGGGGGGGCTCTGGGCCGCCCCCGGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTAAAAAAAGGCCTGACATTACGCCAg                             <  1:4043881/147‑1 (MQ=38)
                                                                                                                               gggggTGCAGGGGGCGGCGGCGATTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTAAAAAAAGGCCTGACATTACGCCAGGcc                          >  2:693120/1‑150 (MQ=255)
                                                                                                                                   gcgctggggggggCGGGGTTGGCCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTAAAAAAAGGCCTGACATTACGCCAGGCCTTCt                      <  1:850658/145‑1 (MQ=38)
                                                                                                                                     gctgggggCGGCGGCTTTGGGCCGCCCCCGGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTAAAAAAAGGCCTGGCGTAATGTCAGGCCtttttt                    <  1:2024341/147‑5 (MQ=37)
                                                                                                                                     gCAGGGGGCGGCGGCGATTGGCCGCCCCCAGCGCGCTCCTTTCGCCAGTGGCAATATGTTTATTAGCTCATGAAAGGAGCGCAACAAAATGATGAATCAACAAATAAAAACAACTTAAAAAAAGGCCTGACATAACGCCAGGCCttaatc                    >  1:10774424/1‑146 (MQ=37)
                                                                                                                                       aGGGGGCGGCGGCGATTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTAAAAAAAGGCCTGACATTACGCCCGGCCTTCTGCGt                  >  2:3981147/1‑150 (MQ=255)
                                                                                                                                          agggggcggccAATCGCCGCCGCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTAAAAAAAGGCCTGACATTACGCCAGGCCTTCTGCGttaa               >  1:11027609/5‑150 (MQ=39)
                                                                                                                                           gggggcggcgTTTGGCCGCCCCCGGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTAAAAAAAGGCCTGACATTACGCCAGGCCTTCTGCGttaat              <  2:1535286/147‑1 (MQ=255)
                                                                                                                                               gcggggTTTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTAAAAAAAGGCCTGACATTACGCCAGGCCTTCTGCGTtaattaat          <  2:4066381/150‑1 (MQ=255)
                                                                                                                                               gcggcgATTGGCCCCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTAAAAAAAGGCCTGACATTACGCCAGGCCTTCTGCGTtaattaat          <  1:7883365/150‑1 (MQ=255)
                                                                                                                                                cggcgATTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTAAAAAAAGGCCTGACATTACGCCAGGCCTTCTGCGTTAATTAATc         >  1:8796362/1‑150 (MQ=255)
                                                                                                                                                 ggcgATTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTAAAAAAATGCCTGACATTACGCCAGGCCTTCTGCGTTAATTAATCa        >  2:9714403/1‑150 (MQ=255)
                                                                                                                                                    gATTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTAAAAAAAGGCCTGACATTACGCCAGGCCTTCTGCGTTAATTAATCACGc     >  1:5523305/1‑150 (MQ=255)
                                                                                                                                                    gATTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTAAAAAAAGGCCTGACATTACGCCAGGCCTTCTGCGTTAATTAATCACGc     >  1:621460/1‑150 (MQ=255)
                                                                                                                                                    gATTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTAAAAAAAGGCCTGACATTACGCCAGGCCTTCTGCGTTAATTAATCACGc     >  1:8462923/1‑150 (MQ=255)
                                                                                                                                                    gATTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTAAAAAAAGGCCTGACATTACGCCAGGCCTTCTGCGTTAATTAATCACGc     >  2:2925540/1‑150 (MQ=255)
                                                                                                                                                    gATTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTAAAAAAAGGCCTGACATTACGCCAGGCCTTCTGCGTTAATTAATCACGc     >  1:10526798/1‑150 (MQ=255)
                                                                                                                                                    gATTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGATGCTAAGCTCATGTAAGGAGCGCAACAAGATGATGAATCAACATAGAACAACATCTTAAAAAAAGGCCTGACATTACTCCAGGCCTACTGCGTTAATGAAACACGc     >  2:5281743/1‑150 (MQ=37)
                                                                                                                                                      ttGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTAAAAAAAGGCCTGACATTACGCCAGGCCTTCTGCGTTAATTAATCACGCtt   >  1:6302782/1‑150 (MQ=255)
                                                                                                                                                       |                                                                                                                                                     
GGGGCAGCCCACATGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTAAAAAAAGGCCTGACATTACGCCAGGCCTTCTGCGTTAATTAATCACGCTTT  >  NC_000913/915066‑915364

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: