Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 915,226 T→G 12.9% intergenic (‑369/+126) ybjE ← / ← aqpZ putative transporter/aquaporin Z

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913915,2260TG12.9% 797.8 / 16.1 425intergenic (‑369/+126)ybjE/aqpZputative transporter/aquaporin Z
Reads supporting (aligned to +/- strand):  ref base T (237/63);  new base G (13/47);  total (301/124)
Fisher's exact test for biased strand distribution p-value = 3.71e-17
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 434 total aligned reads displayed.

CATGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTAAAAAAAGGCCTGACATTACGCCAGGCCTTCTGCGTTAATTAATCACGCTTTTCCAGCAGGGT  >  NC_000913/915077‑915375
                                                                                                                                                      |                                                                                                                                                     
 aTGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCTg                                                                                                                                                      >  1:4773266/1‑150 (MQ=32)
   ccaTGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCTgcg                                                                                                                                                    >  1:3282417/3‑150 (MQ=17)
      tgtgGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCAgcgcgc                                                                                                                                                 >  1:4712184/1‑150 (MQ=17)
         gggCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCTGCGAGCGcc                                                                                                                                              >  2:8478359/1‑150 (MQ=17)
          ggCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCTGCGCGCTCCt                                                                                                                                             >  2:1035562/1‑150 (MQ=32)
           gCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCTGCGCGCACCtt                                                                                                                                            >  1:7697576/1‑150 (MQ=17)
            cTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCCGAGCCCGGGGGTGCAGGGGGCGGCGGCGAGTGGCCGCCCCCTGCGCGCTCCTTg                                                                                                                                           >  1:4300874/1‑150 (MQ=17)
            cTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCTGCGCGCTCCtag                                                                                                                                           >  2:518256/1‑148 (MQ=17)
            cTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCAGCGCGCTCCTTg                                                                                                                                           >  2:9647116/1‑150 (MQ=17)
             tGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCTGCGCGCTCCTTgc                                                                                                                                          >  2:3572680/1‑150 (MQ=32)
                  gcgcGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCTGCGCGCTCCTTGCGCCAg                                                                                                                                     >  2:4881937/1‑150 (MQ=32)
                  gcgcGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCTGCGCGCTCCTTGCGCCAg                                                                                                                                     >  2:840196/1‑150 (MQ=32)
                   ggcgTTTTCCTGGGATGTTACCACGCGCCCGACCGGGTCGCGTTATGGTATATGTCGTGGCCACCGCGCAGCGGCTTTTTTGTTCGCCCGAGCCGGGGGGTGCCGGGGGCGGGGGCTTTGGGCCGCCCCCTGCGCGCTCCTTGCGCCAGt                                                                                                                                    <  2:4089396/149‑1 (MQ=2)
                   cgcgTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGt                                                                                                                                    >  1:10608084/1‑150 (MQ=32)
                      gTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGc                                                                                                                                 >  2:10535832/1‑150 (MQ=33)
                       ttttACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGcc                                                                                                                                >  1:3829416/1‑149 (MQ=18)
                       ttttACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCa                                                                                                                                >  2:3013087/1‑150 (MQ=34)
                          tACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGATGCAGGGGGCGGCGGCGATTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAata                                                                                                                             >  1:2116243/1‑150 (MQ=37)
                             aGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGt                                                                                                                          >  2:10805068/1‑150 (MQ=39)
                             aGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGt                                                                                                                          >  1:7457723/1‑150 (MQ=39)
                             aGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGt                                                                                                                          >  1:1268171/1‑150 (MQ=39)
                             aGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCGGCGCGCTCCTTGCGCCACTGGCAATATGt                                                                                                                          >  1:5309993/1‑150 (MQ=255)
                             aGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGAGTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGt                                                                                                                          >  1:3028730/1‑150 (MQ=255)
                              gggATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGtt                                                                                                                         >  2:725202/1‑150 (MQ=40)
                               gtttGTAACCTCGCGCCCGACCGGGTAGCCGTACGGTATAAGCGGAGGGCCCCGGGCCGCGGCTATTTTGTCCGCCAGAGCCGGGGGGTGCTGGGGGCGGCGGCTTTGGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTg                                                                                                                        <  2:1864152/147‑1 (MQ=11)
                               ggATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTg                                                                                                                        >  2:6747107/1‑150 (MQ=40)
                               ggATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTg                                                                                                                        >  1:6818761/1‑150 (MQ=40)
                               ggATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCAGCGCGCTCCTTGCGCCAGTGGCAATATGTTg                                                                                                                        >  1:358070/1‑150 (MQ=255)
                                     tACCCCGCCCCCGACCGGGTCGCTGTATGGTATCTGGCGGGGCCCCCGCGCCGGGGCTTTTTTGTCCCCCCGCGCGGGGGGGGGCGGGGGGGGGGGGCTTTGGGCCCCCCCCGCCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGc                                                                                                                  <  2:6556566/150‑1 (MQ=255)
                                      aCCTCGCGCCCGACCGGGTCGCCGTACGGGTTAAGGGGTGGGCACCGCGCCGGGGCGTTTTTGTTCGCCCGCGCCGGGGGGTGCTGGGGGCGGCGGCGTTTGGCCGCCCCGGGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCt                                                                                                                 <  2:6845842/150‑1 (MQ=255)
                                         tCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCAGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCaa                                                                                                              >  1:9048358/1‑149 (MQ=255)
                                          cgcgCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATg                                                                                                             >  2:1539778/1‑150 (MQ=255)
                                          cgcgCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCAGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATg                                                                                                             >  2:4306104/1‑150 (MQ=255)
                                          cgcgCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGAGTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATg                                                                                                             >  2:1493860/1‑150 (MQ=255)
                                               ccGACCGGGTCGCCGTATGGTTTAAGTCGTGGCCACCGCGCCGGGGCTTTTTTGTCCGCCAGAGCCGGGGGGTGCTGGGGGCGGCGGCGTTGGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAgg                                                                                                        <  2:2933110/150‑1 (MQ=17)
                                               ccGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCTGCGCGCTCCGTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAgg                                                                                                        >  1:2582553/1‑150 (MQ=255)
                                                  aCCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAgc                                                                                                     >  2:8894485/1‑150 (MQ=255)
                                                  aCCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCAGCGCGCTCCGTGCGCCAGTGGCCATATGTTGCTTAGGTCATGAAAGGAgc                                                                                                     >  1:2852693/1‑150 (MQ=255)
                                                   cccGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCAGCGCGCACCATGCGCCAGTGGCAATATGTTTCTTATCTCAAGAAAGGAgcg                                                                                                    >  1:9596489/1‑150 (MQ=17)
                                                   cccGGAAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCACGGGGCGGCGGCGAGTGTCCCCCCCCAGCCCGCACCAAGCGCCCCGGGCAAAATGTTGCTTATCTAAATAAAGGAgag                                                                                                    >  1:1091614/1‑148 (MQ=14)
                                                    ccGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCAGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCACATGAAAGGAgcgc                                                                                                   >  1:1885266/1‑150 (MQ=255)
                                                    ccGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCAGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCGTAGCTCATTAAAGGAgcgc                                                                                                   >  1:10050833/1‑150 (MQ=255)
                                                    ccGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCAGCGCGCGCCGTGCGCCAGTGGCAATATGTTGCTTAGCTCATGGAAGGAgcgc                                                                                                   >  1:10356055/1‑150 (MQ=255)
                                                    ccGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGAATGGCCGCCCCCAGCGCGCTCCTTGCGCCAGTGGCAAAATGTTTCTTAGCTCATGAAAGGAgcgc                                                                                                   >  2:9338291/1‑150 (MQ=255)
                                                             gTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGCCCGCGATTGGCCGCCCCCAGCCCCCACCATGCGCCCCTGGCAAAAAGTTTCTTAGCGCAAGAAAGGCGCCcaacaaaaag                                                                                          >  1:7272980/1‑146 (MQ=14)
                                                              tAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCCGCGCTTGGCCGCCCCCAGCGCCCGCCGTGCGCCACTGGCAAAATGTTGCTTAGCTCAAGAAAGGAGCGCAACAAgatgt                                                                                         >  1:9773053/1‑149 (MQ=18)
                                                                   gATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAAt                                                                                    >  2:1381586/1‑150 (MQ=255)
                                                                   gATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAAt                                                                                    >  2:11675579/1‑150 (MQ=255)
                                                                   gATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAACTCATGACAGGAGCGCAACAAGATGATGAAt                                                                                    >  2:5460278/1‑150 (MQ=255)
                                                                   gATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCCCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAACTCATGAAAGGAGCGCAACAAGATGATGAAt                                                                                    >  2:2657094/1‑150 (MQ=255)
                                                                    aTAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATc                                                                                   >  2:1594116/1‑150 (MQ=255)
                                                                       cgTCGCGGGCACCGCGCCGCGGCGATTTTGTTCGCCAGAGCCGGGGGGTGCTGGGGGGGGGGGCGATTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAAc                                                                                <  2:6186670/149‑1 (MQ=18)
                                                                        gTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCAGCGCCCTCCGTGCCCCAGTGGCCAAAAGTTGCCTAGCTCAGGAAAGGAGCCCAACAAGAAGATGTAacaaca                                                                               >  2:11090068/1‑150 (MQ=17)
                                                                         tCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTTCTTATCTCATGAAAGGAGCGCAACAAGATGATTAATAAACat                                                                              >  2:739/1‑150 (MQ=255)
                                                                           gAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACatat                                                                            >  2:600689/1‑150 (MQ=255)
                                                                           gAGGGCACCGCGCAGCGGCGATTTTGTTAGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCTGCGCGCACCTTGCGCCAGTGGCAATATGTTGAATAGCTCATGAAAGGAGCGCAACAAGATGATGAATaaaaaaaa                                                                            >  2:3157985/1‑145 (MQ=17)
                                                                                   cgcgCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACAt                                                                    >  2:5750677/1‑150 (MQ=255)
                                                                                     cgcAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCt                                                                  >  2:10970142/1‑150 (MQ=255)
                                                                                           ggcgATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTaaaaa                                                            >  2:4633533/1‑150 (MQ=255)
                                                                                           ggcgATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCCCCCCCAGCGCGCGCCGTGCGCCCCGGGCCCAAAGTTGCTTAGCGCAGGCAAGGCGCGCAACAAGATGATGAAACAACAAAAAACAACAACAGaaaaa                                                            >  1:6511571/1‑150 (MQ=12)
                                                                                            gggtttttGTTTCGCCCGCGCCCGGGGGTGCTGGGGGCGGCGGCGATGGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTaaaaa                                                            <  2:8125176/146‑1 (MQ=25)
                                                                                              gtttttGGTCGCCAGCGCCGGGGGGGGCGGGGGGGGGGGGGGTGTGGCCCCCCCCGGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTAAAAAAAg                                                         <  2:12001080/148‑1 (MQ=255)
                                                                                               aTTTTGTTCGCCAGAGCCGGGGGGTGCTGGGGGGGGCGGCGATTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTAAAAAAAgg                                                        <  2:977988/150‑1 (MQ=255)
                                                                                                ttttttTCGCCTGCGCGGGGGGGGGGTGGGGGGGGGGGGTTTGGGCCCCCCCGGGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTAAAAAAAGGc                                                       <  2:2548261/150‑1 (MQ=255)
                                                                                                 tttGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTAAAAAAAGGcc                                                      >  2:2816315/1‑150 (MQ=255)
                                                                                                    gTTCGCCTGCGCGGGGGGGTGGTGGGGGGGGGGGCTTTTGGCCGCCCCCCGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTAAAAAAAGGCCTGa                                                   <  2:10652857/150‑1 (MQ=37)
                                                                                                      ttCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTAAAAAAAGGCCTGAc                                                  >  2:641919/1‑150 (MQ=255)
                                                                                                            agagCCCGGGGGTGCATGGGGCGGCGGCGATTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTAAAAAAAGGCCTGACATTACg                                            >  1:2265132/1‑150 (MQ=255)
                                                                                                               gggggggggTGGGGGGGGGGGGGGCTTTGGCCCGCCCCCGGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTAAAAAAAGGCCTGACATTACGCCa                                         <  2:2123936/146‑1 (MQ=255)
                                                                                                               gcgggggggTGCTGGGGGGGGGGGCTTTGGGCCGCCCCCGCCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTAAAAAAAGGCCTGACATTACGCCa                                         <  2:7781456/146‑1 (MQ=255)
                                                                                                               gCCGGGGGGTGCTGGGGGCGGCGGCGTTTGGCCGCCCCCGGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTAAAAAAAGGCCTGACATTACGCCa                                         <  2:10025249/150‑1 (MQ=255)
                                                                                                                ccgggggggGCCGGGGGCGGGGGCTTTGGCCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTAAAAAAAGGCCGGACATTACGCCAg                                        <  2:8836502/147‑1 (MQ=37)
                                                                                                                  ggggggTGGGGGGGGGGGGGGGGGTGGCCCGCCCCCGGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTAAAAAAAGGCCTGACATTACGCCAGGc                                      <  2:10284273/149‑1 (MQ=255)
                                                                                                                  ggggggTGCTGGGGGGGGGGGTTTTTGGCCGCCCCGTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTAAAAAAAGGCCTGACATTACGCCAGGc                                      <  2:8703016/149‑1 (MQ=255)
                                                                                                                   gggggTGCAGGGGGCGGCGGCGATTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTAAAAAAAGGCCTGACATTACGCCAGGcc                                     >  2:11035465/1‑150 (MQ=255)
                                                                                                                       gcgctggggggggCGGGGTTGGCCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTAAAAAAAGGCCTGACATTACGCCAGGCCTTCt                                 <  1:850658/145‑1 (MQ=38)
                                                                                                                       gTGCAGGGGGCGGCGGCGATTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATTATGAATCAACATATAACAACATCTTAAAAAAAGGCCTGACATTACGCCAGGCCTTCt                                 <  2:8232183/150‑1 (MQ=255)
                                                                                                                          cAGGGGGCGGCGGCGATTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTAAAAAAAGGCCTGACATTACGCCAGGCCTTCTGCg                              >  1:10775040/1‑150 (MQ=255)
                                                                                                                           aGGGGGCGGCGGCGATTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTAAAAAAAGGCCTGACATTACGCCCGGCCTTCTGCGt                             >  2:3981147/1‑150 (MQ=255)
                                                                                                                             ggggCGGCGGCTCTTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTAAAAAAAGGCCTGACATTACGCCAGGCCTTCTGCGtta                           <  2:3802610/150‑1 (MQ=255)
                                                                                                                             ggggCGGCGGCGATTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATGTAACAACATCTTAAAAAAAGGCCTGACATTACGCCAGGCCTTCTGCGtta                           >  1:7864871/1‑150 (MQ=255)
                                                                                                                                gcggcggcgATTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTAAAAAAAGGCCTGACATTACGCCAGGCCTTCTGCGtgaatt                        >  2:7055324/1‑150 (MQ=255)
                                                                                                                                    cggcgATTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTAAAAAAAGGCCTGACATTACGCCAGGCCTTCTGCGTTAATTAATc                    >  1:8796362/1‑150 (MQ=255)
                                                                                                                                       gtttgggCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTAAAAAAAGGCCTGACATTACGCCAGGCCTTCTGCGTTAATTAATCACg                 <  1:8075512/145‑1 (MQ=255)
                                                                                                                                        gATTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACGACATCTTAAAAAAAGGCCTGACATTACGCCAGGCCTTCTGCGTTAATTAATCACGc                >  1:11168144/1‑150 (MQ=255)
                                                                                                                                        gATTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTAAAAAAAGGCCTGACATTACGCCAGGCCTTCTGCGTTAATTAATCACGc                >  1:621460/1‑150 (MQ=255)
                                                                                                                                        gATTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTAAAAAAAGGCCTGACATTACGCCAGGCCTTCTGCGTTAATTAATCACGc                >  1:8462923/1‑150 (MQ=255)
                                                                                                                                        gATTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTAAAAAAAGGCCTGACATTACGCCAGGCCTTCTGCGTTAATTAATCACGc                >  1:5523305/1‑150 (MQ=255)
                                                                                                                                          ttGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTAAAAAAAGGCCTGACATTACGCCAGGCCTTCTGCGTTAATTAATCACGCtt              >  1:6302782/1‑150 (MQ=255)
                                                                                                                                             gccgccCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTAAAAAAAGGCCTGACATTACGCCAGGCCTTCTGCGTTAATTAATCACGCTTTTc           <  2:4970338/150‑1 (MQ=255)
                                                                                                                                             gccgccCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACAGCTTAAAAAAAGGCCTGACATTACGCCAGGCCTTCTGCGTTAATTAATCACGCTTTTc           >  2:11838644/1‑150 (MQ=255)
                                                                                                                                             gccgccCCCGGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTAAAAAAAGGCCTGACATTACGCCAGGCCTTCTGCGTTAATTAATCACGCTTTTc           <  2:11264795/150‑1 (MQ=255)
                                                                                                                                               cgccCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTAAAAAAAGGCCTGACATTACGCCAGGCCTTCTGCGTTAATTAATCACGCTTTTCca         <  2:7681826/150‑1 (MQ=255)
                                                                                                                                                ccccccGCCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTAAAAAAAGGCCTGACATTACGCCAGGCCTTCTGCGTTAATTAATCACGCTTTTCcag        <  1:10494419/149‑1 (MQ=255)
                                                                                                                                                 ccccccccGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTAAAAAAAGGCCTGACATTACGCCAGGCCTTCTGCGTTAATTAATCACGCTTTTCcagc       <  1:3124727/150‑1 (MQ=255)
                                                                                                                                                  ccccTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTAAAAAAAGGCCTGACATTACGCCAGGCCTTCTGCGTTAATTAATCACGCTTTTCcagca      <  2:7908345/150‑1 (MQ=255)
                                                                                                                                                  ccccTGCGCGCTCCGTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTAAAAAAAGGCCTGACATTACGCCAGGCCTTCTGCGTTAATTAATCACGCTTTTCcagca      <  2:9043664/150‑1 (MQ=255)
                                                                                                                                                  ccccCGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTAAAAAAAGGCCTGACATTACGCCAGGCCTTCTGCGTTAATTAATCACGCTTTTCcagca      <  1:11667721/150‑1 (MQ=255)
                                                                                                                                                    ccTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTAAAAAAAGGCCTGACATTACGCCAGGCCTTCTGCGTTAATTAATCACGCTTTTCCAGCAgg    >  2:4137741/1‑150 (MQ=255)
                                                                                                                                                      tGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTAAAAAAAGGCCTGACATTACGCCAGGCCTTCTGCGTTAATTAATCACGCTTTTCCAGCAGGGt  >  2:12046841/1‑150 (MQ=255)
                                                                                                                                                      |                                                                                                                                                     
CATGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTAAAAAAAGGCCTGACATTACGCCAGGCCTTCTGCGTTAATTAATCACGCTTTTCCAGCAGGGT  >  NC_000913/915077‑915375

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: