Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 915,227 G→C 5.2% intergenic (‑370/+125) ybjE ← / ← aqpZ putative transporter/aquaporin Z

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913915,2270GC 5.2% 1283.3 / 9.9 418intergenic (‑370/+125)ybjE/aqpZputative transporter/aquaporin Z
Reads supporting (aligned to +/- strand):  ref base G (285/106);  new base C (3/22);  total (290/128)
Fisher's exact test for biased strand distribution p-value = 1.34e-09
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 439 total aligned reads displayed.

ATGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTAAAAAAAGGCCTGACATTACGCCAGGCCTTCTGCGTTAATTAATCACGCTTTTCCAGCAGGGTC  >  NC_000913/915078‑915376
                                                                                                                                                      |                                                                                                                                                     
aTGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCTg                                                                                                                                                       >  1:7625840/1‑150 (MQ=32)
aTGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCTg                                                                                                                                                       >  1:8419562/1‑150 (MQ=32)
aTGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCTg                                                                                                                                                       >  1:4773266/1‑150 (MQ=32)
  ccaTGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCTgcg                                                                                                                                                     >  2:2197188/3‑150 (MQ=17)
     tgtgGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCAgcgcgc                                                                                                                                                  >  1:4712184/1‑150 (MQ=17)
         ggCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCTGCGCGCTCCt                                                                                                                                              >  2:6230225/1‑150 (MQ=32)
         ggCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCTGCGCGCTCCt                                                                                                                                              >  2:1035562/1‑150 (MQ=32)
           cTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCCGAGCCCGGGGGTGCAGGGGGCGGCGGCGAGTGGCCGCCCCCTGCGCGCTCCTTg                                                                                                                                            >  1:4300874/1‑150 (MQ=17)
           cTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCTGCGCGCTCCtag                                                                                                                                            >  2:518256/1‑148 (MQ=17)
            tGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCTGCGCGCTCCTTgc                                                                                                                                           >  2:3572680/1‑150 (MQ=32)
              aGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCTGCGCGCTCCTTgcgc                                                                                                                                         >  1:3037565/1‑150 (MQ=32)
                 gcgcGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCTGCGCGCTCCTTGCGCCAg                                                                                                                                      >  2:840196/1‑150 (MQ=32)
                 gcgcGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCTGCGCGCTCCTAGCGCCAg                                                                                                                                      >  2:7742239/1‑150 (MQ=17)
                 gcgcGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCAGCGCGCTCCTTGCGcccg                                                                                                                                      >  2:1544792/1‑148 (MQ=17)
                  ggctttttACTGGTATGTTCCGTGGCGCCCCCCCGGGTCGCTGTATGGTATACGTCGCGGGCACCGCGAGGCGGATTTTTTGTTCGCCAGCGCGGGGGGGTGCTGGGGGCGGGGGGGATGGGCCGCCCCCGGCGCGCTCCTTGCGCCAGt                                                                                                                                     <  2:5021952/146‑1 (MQ=2)
                  ggcgTTTTCCTGGGATGTTACCACGCGCCCGACCGGGTCGCGTTATGGTATATGTCGTGGCCACCGCGCAGCGGCTTTTTTGTTCGCCCGAGCCGGGGGGTGCCGGGGGCGGGGGCTTTGGGCCGCCCCCTGCGCGCTCCTTGCGCCAGt                                                                                                                                     <  2:4089396/149‑1 (MQ=2)
                  cgcgTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGt                                                                                                                                     >  1:10608084/1‑150 (MQ=32)
                     gTTTTACTGGGATGTTACCCCGCGCCCGACCGGGTTGCCTTACGGGATAAGTCGCGGGCACCGCGCCGCGGCGTTTTTGTTCGCCAGAGCCGGGGGGGGCAGGGGGCGGCGGCTATTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGc                                                                                                                                  <  2:9036374/150‑1 (MQ=11)
                     gTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGc                                                                                                                                  >  2:10535832/1‑150 (MQ=33)
                      ttttACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCa                                                                                                                                 >  2:3013087/1‑150 (MQ=34)
                      ttttACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCa                                                                                                                                 >  1:3363707/1‑150 (MQ=34)
                        ttACGGGGTTGTGCCCGCCCGCCCGACCGGGTCGCCGTCCGGCTTACGGCGGGGGCACCGGGCTGCGGCTTTTTGTTGCGCACGGGCCGGGGGGGGCGGGGGGGGGCGGGTTGTGCCCCCCCCCGGCGCGCTCCTTGCGCCAGTGGCAat                                                                                                                               <  2:9620215/150‑1 (MQ=255)
                         tACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGATGCAGGGGGCGGCGGCGATTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAata                                                                                                                              >  1:2116243/1‑150 (MQ=37)
                            ngggATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCGGCGCGCTCCGGGCGCCAGGGGCCATATgg                                                                                                                           >  1:6299372/2‑149 (MQ=11)
                            aGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGt                                                                                                                           >  1:7457723/1‑150 (MQ=39)
                            aGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGt                                                                                                                           >  2:10805126/1‑150 (MQ=39)
                            aGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCGGCGCGCTCCTTGCGCCACTGGCAATATGt                                                                                                                           >  1:5309993/1‑150 (MQ=255)
                            aGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGAGTGGCCGCCCCCGGCGCGCTCCATGCGCCAGTGGCCATATGt                                                                                                                           >  2:9270628/1‑150 (MQ=255)
                             gggTTGTCACCGCGCCCCCGCCCGGGTCCCCTTATGGTATAGGTGGGGGCCCCCGCGCCGGGGCTTTTTTGTGCCCCCGCGCGGGGGGGGGGGGGGGGGGGGGGGGTTGGGCCCCCCCCGCCGCGCTCCTTGCGCCAGTGGCAATATGtt                                                                                                                          <  2:453960/150‑1 (MQ=255)
                               tttGTTACCGCGCGCCCGACCCGGTCGCGTTATGGTATATGTCGGGGGCCCCGCGCGGGGGGTTTTTGGTTCGCCTGCGCCGGGGGGGGGGGGGGGCGGGGGGGTTTGGCCGCCCCCGGCGCGCTCCTTGCGCCAGTGGCAATATGTTGc                                                                                                                        <  2:6108900/148‑1 (MQ=9)
                                    tACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGc                                                                                                                   >  2:4930703/1‑150 (MQ=255)
                                     aCCTCGCGCCCGACCGGGTAGCCGTATGGTATAAGTGGAGGGCACCGCGCAGGGGGGTTTTTGTTCGCCTGCGCCGGGGGGTGCTGGGGGCGGCGGCGATTGGCCGCCCCCGGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCt                                                                                                                  <  2:6845788/150‑1 (MQ=14)
                                        tCGCGCCCGACCCGGTAGCGGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGTGGCGGCGGCGATTGGACGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGATCAt                                                                                                               >  2:1933761/1‑150 (MQ=255)
                                        tCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCAGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCaa                                                                                                               >  1:9048358/1‑149 (MQ=255)
                                         cgcgCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATg                                                                                                              >  2:1493903/1‑150 (MQ=255)
                                          gcgcCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGa                                                                                                             >  2:888155/1‑150 (MQ=255)
                                              ccGACCGGGTCGCCGTATGGTTTAAGTCGTGGCCACCGCGCCGGGGCTTTTTTGTCCGCCAGAGCCGGGGGGTGCTGGGGGCGGCGGCGTTGGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAgg                                                                                                         <  2:2933110/150‑1 (MQ=17)
                                              ccGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCTGCGCGCTCCGTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAgg                                                                                                         >  1:2582553/1‑150 (MQ=255)
                                                  cccGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCAGCGCGCACCATGCGCCAGTGGCAATATGTTTCTTATCTCAAGAAAGGAgcg                                                                                                     >  1:9596489/1‑150 (MQ=17)
                                                  cccGGAAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCACGGGGCGGCGGCGAGTGTCCCCCCCCAGCCCGCACCAAGCGCCCCGGGCAAAATGTTGCTTATCTAAATAAAGGAgag                                                                                                     >  1:1091614/1‑148 (MQ=14)
                                                   ccGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCAGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCACATGAAAGGAgcgc                                                                                                    >  1:1885266/1‑150 (MQ=255)
                                                   ccGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCAGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCGTAGCTCATTAAAGGAgcgc                                                                                                    >  1:10050833/1‑150 (MQ=255)
                                                   ccGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCAGCGCGCGCCGTGCGCCAGTGGCAATATGTTGCTTAGCTCATGGAAGGAgcgc                                                                                                    >  1:10356055/1‑150 (MQ=255)
                                                           agTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCAGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCAGGAAAGGAGCGCAACAAgat                                                                                            >  2:881754/2‑150 (MQ=255)
                                                             tAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCAGCGCGCTCCTAGCGCCACTGGCAATATGTTTCATAGCTCATGAAAGGAGCCCAACAAcatta                                                                                          >  1:7205225/1‑148 (MQ=17)
                                                             tAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCAGCGCGCGCCATGCGCCACTGGCAAAAATTTTCTTAGCTCATGAAAGGAGCGCAACAAcatga                                                                                          >  1:1303657/1‑150 (MQ=17)
                                                             tAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCCGCGCTTGGCCGCCCCCAGCGCCCGCCGTGCGCCACTGGCAAAATGTTGCTTAGCTCAAGAAAGGAGCGCAACAAgatgt                                                                                          >  1:9773053/1‑149 (MQ=18)
                                                                  gATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAAt                                                                                     >  2:11105239/1‑150 (MQ=255)
                                                                  gATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAAt                                                                                     >  2:1381586/1‑150 (MQ=255)
                                                                  gATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCTGCGCGCTCCTTGCGCCACTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAAt                                                                                     >  2:5800926/1‑150 (MQ=255)
                                                                  gATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCCCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAACTCATGAAAGGAGCGCAACAAGATGATGAAt                                                                                     >  2:2657094/1‑150 (MQ=255)
                                                                   aTAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATc                                                                                    >  2:1594116/1‑150 (MQ=255)
                                                                        tCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGGCGGGGGGGGCGGGGCTGGGGCGCCCCCCGCGCGCGCCATGCGCCCGAGGCAAAAAGTTGCTTAGGGCCGGTCAGGGGCGCAACACAATGTTGAATCAAcca                                                                               >  1:2405961/1‑148 (MQ=9)
                                                                        tCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTTCTTATCTCATGAAAGGAGCGCAACAAGATGATTAATAAACat                                                                               >  2:739/1‑150 (MQ=255)
                                                                        tCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAACGAGCGCAACAAGATGATGAATCAACat                                                                               >  2:8453831/1‑150 (MQ=255)
                                                                          gAGGGCACCGCGCAGCGGCGATTTTGTTAGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCTGCGCGCACCTTGCGCCAGTGGCAATATGTTGAATAGCTCATGAAAGGAGCGCAACAAGATGATGAATaaaaaaaa                                                                             >  2:3157985/1‑145 (MQ=17)
                                                                                    cgcAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCt                                                                   >  2:10970142/1‑150 (MQ=255)
                                                                                      cAGCGGCGATTTTGTTCGCCAGAGCCGGGGGGTGCTGGGGGCGGCGGCGATTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTa                                                                 <  2:7042291/150‑1 (MQ=255)
                                                                                          ggcgATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTaaaaa                                                             >  2:4633533/1‑150 (MQ=255)
                                                                                          ggcgATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCCCCCCCAGCGCGCGCCGTGCGCCCCGGGCCCAAAGTTGCTTAGCGCAGGCAAGGCGCGCAACAAGATGATGAAACAACAAAAAACAACAACAGaaaaa                                                             >  1:6511571/1‑150 (MQ=12)
                                                                                             gtttttGGTCGCCAGCGCCGGGGGGGGCGGGGGGGGGGGGGGTGTGGCCCCCCCCGGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTAAAAAAAg                                                          <  2:12001080/148‑1 (MQ=255)
                                                                                               ttttttTCGCCTGCGCGGGGGGGGGGTGGGGGGGGGGGGTTTGGGCCCCCCCGGGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTAAAAAAAGGc                                                        <  2:2548261/150‑1 (MQ=255)
                                                                                               ttttGTGCCCCCGCGCCCGGGGGTGCGGGGGGCGGCGGCGATGGCCCCCCCCCCGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTAAAAAAAGGc                                                        <  2:6525083/150‑1 (MQ=25)
                                                                                                tttGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTAAAAAAAGGcc                                                       >  2:2816315/1‑150 (MQ=255)
                                                                                                   gTTCGCCTGCGCGGGGGGGTGGTGGGGGGGGGGGCTTTTGGCCGCCCCCCGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTAAAAAAAGGCCTGa                                                    <  2:10652857/150‑1 (MQ=37)
                                                                                                    ttCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTAAAAAAAGGCCTGAc                                                   >  2:8629249/1‑150 (MQ=255)
                                                                                                    gtccGCCCGCGCCGGGGGGTGGGGGGGGGGGGGGGGTTGGGCCGCCCGCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTAAAAAAAGGCCTGa                                                    <  2:7660748/147‑1 (MQ=37)
                                                                                                              gcgggggggTGGGGGGGGGGGGGGGGTTGGGCCGCCCCCGGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTAAAAAAAGGCCTGACATTACGCCa                                          <  2:71579/146‑1 (MQ=255)
                                                                                                              gCCGGGGGGTGCTGGGGGCGGGGGCTTTTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTAAAAAAAGGCCTGACATTACGCCa                                          <  2:3982463/150‑1 (MQ=38)
                                                                                                              gCCGGGGGGTGCTGGGGGCGGCGGCGTTTGGCCGCCCCCGGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTAAAAAAAGGCCTGACATTACGCCa                                          <  2:10025249/150‑1 (MQ=255)
                                                                                                              gCCGGGGGGTGCTGGGGGCGGCGGCGATTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTAAAAAAAGGCCTGACATTACGCCa                                          <  2:8121185/150‑1 (MQ=255)
                                                                                                               cggggggggGCGGGGGGGGGGGGCTCTGGGCCGCCCCCGGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTAAAAAAAGGCCTGACATTACGCCAg                                         <  1:4043881/147‑1 (MQ=38)
                                                                                                                 ggggggTGGGGGGGGGGGGGGGGGTGGCCCGCCCCCGGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTAAAAAAAGGCCTGACATTACGCCAGGc                                       <  2:10284273/149‑1 (MQ=255)
                                                                                                                  gggggTGCAGGGGGCGGCGGCGATTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTAAAAAAAGGCCTGACATTACGCCAGGcc                                      >  2:11035465/1‑150 (MQ=255)
                                                                                                                      gcgctggggggggCGGGGTTGGCCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTAAAAAAAGGCCTGACATTACGCCAGGCCTTCt                                  <  1:850658/145‑1 (MQ=38)
                                                                                                                      gTGCAGGGGGCGGCGGCGATTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATTATGAATCAACATATAACAACATCTTAAAAAAAGGCCTGACATTACGCCAGGCCTTCt                                  <  2:8232183/150‑1 (MQ=255)
                                                                                                                         cggggggCGGCGGCCTTGCCCCCCCCCCCCCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTAAAAAAAGGCCTGACATTACGCCAGGCCTTCTGCg                               <  1:5489997/148‑1 (MQ=255)
                                                                                                                         cAGGGGGCGGCGGCGATTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTAAAAAAAGGCCTGACATTACGCCAGGCCTTCTGCg                               >  1:10775040/1‑150 (MQ=255)
                                                                                                                            ggggCGGCGGCTCTTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTAAAAAAAGGCCTGACATTACGCCAGGCCTTCTGCGtta                            <  2:3802610/150‑1 (MQ=255)
                                                                                                                            ggggCGGCGGCGATTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATGTAACAACATCTTAAAAAAAGGCCTGACATTACGCCAGGCCTTCTGCGtta                            >  1:7864871/1‑150 (MQ=255)
                                                                                                                                   cggcgATTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTAAAAAAAGGCCTGACATTACGCCAGGCCTTCTGCGTTAATTAATc                     >  1:8796362/1‑150 (MQ=255)
                                                                                                                                    ggcgATTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTAAAAAAATGCCTGACATTACGCCAGGCCTTCTGCGTTAATTAATCa                    >  2:9714403/1‑150 (MQ=255)
                                                                                                                                      gtttgggCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTAAAAAAAGGCCTGACATTACGCCAGGCCTTCTGCGTTAATTAATCACg                  <  1:8075512/145‑1 (MQ=255)
                                                                                                                                      gtttgggCCGCCCCCCGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTAAAAAAAGGCCTGACATTACGCCAGGCCTTCTGCGTTAATTAATCACg                  <  1:6710174/145‑1 (MQ=255)
                                                                                                                                       gATTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACGACATCTTAAAAAAAGGCCTGACATTACGCCAGGCCTTCTGCGTTAATTAATCACGc                 >  1:11168144/1‑150 (MQ=255)
                                                                                                                                          tGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTAAAAAAAGGCCTGACATTACGCCAGGCCTTCTGCGTTAATTAATCACGCttt              >  2:11682539/1‑150 (MQ=255)
                                                                                                                                          gggCCGCCCCGGGCGCGCGCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTAAAAAAAGGCCTGACATTACGCCAGGCCTTCTGCGTTAATTAATCACGCttt              <  1:6120005/149‑1 (MQ=255)
                                                                                                                                            gccgccCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTAAAAAAAGGCCTGACATTACGCCAGGCCTTCTGCGTTAATTAATCACGCTTTTc            >  2:6699144/1‑150 (MQ=255)
                                                                                                                                            gccgccCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACAGCTTAAAAAAAGGCCTGACATTACGCCAGGCCTTCTGCGTTAATTAATCACGCTTTTc            >  2:11838644/1‑150 (MQ=255)
                                                                                                                                            gccgccCCCGGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTAAAAAAAGGCCTGACATTACGCCAGGCCTTCTGCGTTAATTAATCACGCTTTTc            <  2:11264795/150‑1 (MQ=255)
                                                                                                                                             ccgccCCCACCGCGCTCCATGCGCCAGTGGCAATATGTTGCTTAGCTCAGGAAAGGAGCGCAACAAGATGATGAATCAACAAATAACAACATCTTAAAAAAAGGCCTGACATTACGCCAGGCCTTCAGCGTTCATTAATCACGCTTTTcc           >  2:5465227/1‑150 (MQ=255)
                                                                                                                                               gccCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTAAAAAAAGGCCTGACATTACGCCAGGCCTTCTGCGTTAATTAATCACGCTTTTCcag         >  2:12050720/1‑150 (MQ=255)
                                                                                                                                               ccccccGCCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTAAAAAAAGGCCTGACATTACGCCAGGCCTTCTGCGTTAATTAATCACGCTTTTCcag         <  1:10494419/149‑1 (MQ=255)
                                                                                                                                                ccccccccGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTAAAAAAAGGCCTGACATTACGCCAGGCCTTCTGCGTTAATTAATCACGCTTTTCcagc        <  1:3124727/150‑1 (MQ=255)
                                                                                                                                                ccccccGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTAAAAAAAGGCCTGACATTACGCCAGGCCTTCTGCGTTAATTAATCACGCTTTTCcagc        <  1:3617492/150‑1 (MQ=255)
                                                                                                                                                 ccccTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTAAAAAAAGGCCTGACATTACGCCAGGCCTTCTGCGTTAATTAATCACGCTTTTCcagca       <  2:7908345/150‑1 (MQ=255)
                                                                                                                                                 ccccCGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTAAAAAAAGGCCTGACATTACGCCAGGCCTTCTGCGTTAATTAATCACGCTTTTCcagca       <  1:11667721/150‑1 (MQ=255)
                                                                                                                                                   ccTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTAAAAAAAGGCCTGACATTACGCCAGGCCTTCTGCGTTAATTAATCACGCTTTTCCAGCAgg     >  2:4137741/1‑150 (MQ=255)
                                                                                                                                                    cTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGTTGATGAATCAACATATAACAACATCTTAAAAAAAGGCCTGACATTACGCCAGGCCTTCTGCGTTAATTAATCACGCTTTTCCAGCAggg    <  1:2937387/150‑1 (MQ=255)
                                                                                                                                                      gcgcgcTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGCTGAATCAACATATAACAACATCTTAAAAAAAGGCCTGACATTACGCCAGGCCTTCTGCGTTAATTAATCACGCTTTTCCAGCAGGGTc  <  2:6417139/150‑1 (MQ=255)
                                                                                                                                                      |                                                                                                                                                     
ATGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTAAAAAAAGGCCTGACATTACGCCAGGCCTTCTGCGTTAATTAATCACGCTTTTCCAGCAGGGTC  >  NC_000913/915078‑915376

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: