Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,466,630 G→C 6.4% pseudogene (1239/2513 nt) ydbA → pseudogene, autotransporter homolog; interrupted by IS2 and IS30

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,466,6300GC 6.4% 1294.8 / 56.0 395pseudogene (1239/2513 nt)ydbApseudogene, autotransporter homolog; interrupted by IS2 and IS30
Reads supporting (aligned to +/- strand):  ref base G (214/148);  new base C (13/12);  total (234/161)
Fisher's exact test for biased strand distribution p-value = 5.32e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 402 total aligned reads displayed.

ATTGATATCACCGGCGACAGCGCAACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATCGGTATCCAGGTTGACGGCGACCAGGCGGTTGTTAACAACGAAGGCGAGAGCGCCATCACCAACGGTGGCACCGGCACGCAGATTAACGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATTGCTGGCAATAACGGGAAGGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGA  >  NC_000913/1466481‑1466779
                                                                                                                                                     |                                                                                                                                                     
aTTGATATCACCGGCGACAGCGCAACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATCGGTATCCAGGTTGACGGCGACCAGGCGGTTGTTAACAACGAAGGCGAGAGCGCCATCACCAACGGTGGCACCGGCACg                                                                                                                                                       <  2:7504336/150‑1 (MQ=255)
          ccGGCGACAGCGCAACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATCGGTATCCAGGTTGACGGCGACCAGGCGGTTGTTAACAACGAAGGCGAGAGCGCCATCACCAACGGTGGCACCGGCACGCAGATTAACg                                                                                                                                             >  1:10806494/1‑150 (MQ=255)
          ccGGCGACAGCGCAACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATCGGTATCCAGGTTGACGGCGACCAGGCGGTTGTTAACAACGAAGGCGAGAGCGCCATCACCAACGGTGGCACCGGCACGCAGATTAACg                                                                                                                                             >  2:6697742/1‑150 (MQ=255)
            ggCGACAGCGCAACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATCGGTATCCAGGTTGACGGCGACCAGGCGGTTGTTAACAACGAAGGCGAGAGCGCCATCACCAACGGTGGCACCGGCACGCAGATTAACGGt                                                                                                                                           <  2:10864100/150‑1 (MQ=255)
             gCGACAGCGCAACGGTGGATAACAAGGGCACCCTGACCGTCACCGATCCGGAGTCCATCGGGATCCAGGTGGACGGCGACCAGGCGGGTGTTAACAACGAAGGCGAGAGCGCCCTCCCCAACGGTGGCCCCGGCACGCAGAGTAACGGtg                                                                                                                                          >  2:8169105/1‑150 (MQ=18)
                    cgcTACGTTGGATTTCATGGGCTCCATTACCGTCTCCGATCCGGATTCCATCGTTATCCTGGTTGTCGGCGACCAGGCGGTTGTTAACAACGAAGGCGAGAGCGCCATCACCAACGGTGGCACCGGCACGCAGATTAACGGTGATGACGc                                                                                                                                   <  1:8858963/150‑1 (MQ=18)
                       aaCGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATCGGTATCCAGGTTGACGGCGACCAGGCGGTTGTTAACAACGAAGGCGAGAGCGCCATCACCAACGGTGGCACCGGCACGCAGATTAACGGTGATGACGCCAc                                                                                                                                <  2:3196901/150‑1 (MQ=255)
                             ggATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATCGGTATCCAGGTTGACGGCGACCAGGCGGTTGTTAACAACGAAGGCGAGAGCGCCATCACCAACGGTGGCACCGGCACTCAGa                                                                                                                                                   >  1:11650923/1‑125 (MQ=255)
                              gATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATCGGTATCCAGGTTGACGGCGACCAGGCGGTTGTTAATAACGAAGGCGAGAGCGCGATCACCAACGGGGGCACCGGCACGCAGATGAACGGTGAGGACGCCACCGCtaaa                                                                                                                         >  1:5116637/1‑149 (MQ=14)
                              gATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATCGGTATCCAGGTTGACGGCGACCAGGCGGTTGTTAACAACGAAGGCGAGAGCGCCATCACCAACGGTGGCACCGGCACGCAGATTAACGGTGATGACGCCACGGCAaac                                                                                                                         >  1:8272636/1‑150 (MQ=255)
                                   cAAGGGCACCATGACCGTCACCGATCCGGAGTCCATCGGTATCCAGGTTGACGGCGACCAGGCGGTTGTTAACAACGAAGGCGAGAGCGCCATCACCAACGGTGGCACCGGCACGCAGATTAACGGTGATGACGCCACGGCAaacaacaa                                                                                                                    >  2:12155296/1‑150 (MQ=255)
                                   cAAGGGCACCATGACCGTCACCGATCCGGAGTCCATCGGTATCCAGGTTGACGGCGACCAGGCGGTTGTTAACAACGAAGGCGAGAGCGCCATCACCAACGGTGGCACCGGCACGCAGATAAACGGTGATGACGCCACGGCAaaaaacaa                                                                                                                    >  1:2327149/1‑150 (MQ=255)
                                    aaGGGCACCATGACCGTCACCGATCCGGAGTCCATCGGTATCCAGGTTGACGGCGACCAGGCGGTTGTTAACAACGAAGGCGAGAGCGCCATCACCAACGGTGGCACCGGCACGCAGATAAACGGTGATGACGCCACCGCAaaaaacaaa                                                                                                                   >  1:10979980/1‑149 (MQ=255)
                                       ggCACCATGACCGTCACCGATCCGGAGTCCATCGGTATCCAGGTTGACGGCGACCAGGCGGTTGTTAACAACGAAGGCGAGAGCGCCATCACCAACGGTGGCACCGGCACGCGGATTAACGGTGATGACGCCACGGCAAACAACAACGGc                                                                                                                >  2:5076171/1‑150 (MQ=255)
                                           ccATGACCGTCACCGATCCGGAGTCCATCGGTATCCAGGTTGACGGCGACCAGGCGGTTGTTAACAACGAAGGCGAGAGCGCCATCACCAACGGTGGCACCGGCACGCAGATTAACGGTGATGACGCCACGGCAAACAACAACGGCaaaa                                                                                                            <  2:9497936/150‑1 (MQ=255)
                                              tGACCGTCACCGATCCGGAGTCCATCGGTATCCAGGTTGACGGCGACCAGGCGGTTGTTAACAACGAAGGCGAGAGCGCCATCACCAACGGTGGCACCGGCACGCAGATTAACGGTGATGACGCCACGGCAAACAACAACGGCAAAacca                                                                                                         >  1:1701884/1‑150 (MQ=255)
                                                aCCGTCACCGATCCGGAGTCCATCGGTATCCAGGTTGACGGCGACCAGGCGGTTGTTAACAACGAAGGCGAGAGCGCCATCACCAACGGTGGCACCGGCACGCAGATTAACGGTGATGACGCCACGGCAAACAACTACGGCAAAaccacc                                                                                                       <  2:11199137/150‑1 (MQ=255)
                                                     cACCGATCCGGAGTCCATCGGTATCCAGTTTGACGGCGACAAGGCGTTTGTTAAGAACGAAGGCGAGAGCGCCATCACCAACGGTGGCACCGGCACGCAGATTAACGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGa                                                                                                  <  2:2348139/150‑1 (MQ=255)
                                                      aCCGATCCGGAGTCCATCGGTATCCAGGTTGACGGCGACCAGGCGGTTGTTAACAACGAAGGCGAGAGCGCCATCACCAACGGTGGCACCGGCACGCAGATTAACGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGAc                                                                                                 >  2:8332161/1‑150 (MQ=255)
                                                      aCCGATCCGGAGTCCATCGGTATCCAGGTTGACGGCGACCAGGCGGTTGTTAACAACGAAGGCGAGAGCGCCATCACCAACGGTGGCACCGGCACGCAGATTAACGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGAc                                                                                                 >  2:3762628/1‑150 (MQ=255)
                                                           tCCGGAGTCCATCGGTATCCAGGTTGACGGCGACCAGGCGGTTGTTAACAACGAAGGCGAGAGCGCCATCACCAACGGTGGCACCGGCACGCAGATTAACGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCaa                                                                                            >  1:7760972/1‑150 (MQ=255)
                                                             cGGAGTCCATCGGTATCCAGGTTGACGGCGACCAGGCGGTTGTTAACAACGAAGGCGAGAGCGCCATCACCAACGGTGGCACCGGCACGCAGATTAACGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAgg                                                                                          >  1:11833759/1‑150 (MQ=255)
                                                                     aTCGGTATCCAGGTTGACGGCGACCAGGCGGTTGTTAACAACGAAGGCGAGAGCGCCATCACCAACGGTGGCACCGGCACGCAGATTAACGGTGATGACGCCACGGCAAACAACAACGGCAAAaccac                                                                                                        <  2:926923/128‑1 (MQ=255)
                                                                     aTCGGTATCCAGGTTGACGGCGACCAGGCGGTTGTTAACAACGAAGGCGAGAGCGCCATCACCAACGGTGGCACCGGCACGCAGATTAACGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCAcc                                                                                  >  1:8798184/1‑150 (MQ=255)
                                                                     aTCGGTATCCAGGTTGACGGCGACCAGGCGGTTGTTAACAACGAAGGCGAGAGCGCCATCACCAACGGTGGCACCGGCACGCAGATTAACGGTGATGACGCCACGGCAAAAAACAACGGCAAAACCACAGTTGAAGGAAATGATTCCAcg                                                                                  >  1:1107087/1‑149 (MQ=18)
                                                                      tCGGTATCCAGGTTGACGGCGACCAGGCGGTTGTTAACAACGAAGGCGAGAGCGCCATCACCAACGGTGGCACCGGCACGCAGATTAACGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCg                                                                                 >  1:11350788/1‑150 (MQ=255)
                                                                       cGGTATCCAGGTTGACGGCGACCAGGCGGTTGGTAACAACGAAGGCGAGAGCGCCATCACCAACGGTGGCACCGGCACGCAGATTAACGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCgg                                                                                >  2:10033230/1‑150 (MQ=255)
                                                                        ggTATCCAGGTTGACGGCGACCAGGCGGTTGTTAACAACGAAGGCGAGAGCGCCATCACCAACGGTGGCACCGGCACGCAGATTAACGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGt                                                                               <  1:1351424/150‑1 (MQ=255)
                                                                         gTATCCAGGTTGACGGCGACCAGGCGGTTGTTAAAAACGAAGGCGAGAGCGCCATCACCAACGGTGGCACCGGCACGCAGATTAACGGTGAAGACGCCAAGGCAAACAACAACGGCCAAACCACCGTTGACGGCAAGGATTCCACCGGTa                                                                              >  1:11440746/1‑150 (MQ=255)
                                                                           aTCCAGGTTGACGGCGACCAGGCGGTTGTTAACAACGAAGGCGAGAGCGCCATCACCAACGGTGGCACCGGCACGCAGATTAACGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACg                                                                            <  2:4395046/150‑1 (MQ=255)
                                                                           aTCCAGGTTGACGGCGACCAGGCGGTTGTTAACAACGAAGGCGAGAGCGCCATCACCAACGGTGGCACCGGCACGCAGATTAACGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACg                                                                            <  2:11282957/150‑1 (MQ=255)
                                                                              cAGGTTGACGGCGACCAGGCGGTTGTTAACAACGAAGGCGAGAGCGCCATCACCAACGGTGGCACCGGCACGCAGATTAACGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGaa                                                                         >  1:6138041/1‑150 (MQ=255)
                                                                                  ttGACGGCGACCAGGCGGTTGTTAACAACGAAGGCGAGAGCGCCATCACCAACGGTGGCACCGGCACGCAGATTAACGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATTg                                                                     <  1:7114314/150‑1 (MQ=255)
                                                                                   tGACGGCGACCAGGCCGTTGTTAACAACGAAGGCGAGAGCGCAATCACCAAAGGAGGCACCGGCACGCAGATAAACGGTCAGGACGCCACCGCAAACAACACCGGCAAAACCACAGTTGACGGCAAGGAGTACACAGGAACGTACAttac                                                                    >  2:7216488/1‑148 (MQ=255)
                                                                                    gACGGCGACCAGGCGGTTGTTAACAACGAAGGCGAGAGCGCCATCACCAACGGTGGCACCGGCACGCAGATTAACGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATTGCt                                                                   <  2:497699/150‑1 (MQ=255)
                                                                                     aCGGCGACCAGGCGGTTGTTAACAACGAAGGCGAGAGCGCCATCACCAACGGTGGCACCGGCACGCAGATTAACGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATTGCTg                                                                  <  1:6956674/150‑1 (MQ=255)
                                                                                     aCGGCGACCAGGCGGTTGTTAACAACGAAGGCGAGAGCGCCATCACCAACGGTGGCACCGGCACGCAGATTAACGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATACCACCGGTACGGACAATGCTg                                                                  >  1:6473252/1‑150 (MQ=255)
                                                                                     aCGGCGACCAGGCGGTTGTTAACAAAGAAGGCGAGAGCGCCATCACCAAAGGTGGCACCGGCACGAAGATAAAAGGTGAAGACGCCACGGCCAATAACAATGGAAAAACCACAGTTGATGGAAAAGATTCCACCGGTACCAAAATAGcgg                                                                  >  1:4577277/1‑148 (MQ=255)
                                                                                      cGGCGACCAGGCGGTTGTTAACAACGAAGGCGAGAGCGCCATCACCAACGGTGGCACCGGCACGCAGATTAACGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCGAGGATTCCACCGGTACGGAAATTGCTgg                                                                 <  2:2031673/150‑1 (MQ=255)
                                                                                      cGGCGACCAGGCGGTTGTTAACAACGAAGGCGAGAGCGCCATCACCAACGGTGGCACCGGCACGCAGATTAACGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATTGCTgg                                                                 <  2:1757151/150‑1 (MQ=255)
                                                                                       ggCGACCAGGCGGTTGTTAACAACGAAGGCGAGAGCGCCATCACCAACGGTGGCACCGGCACGCAGATTAACGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATTGCTGGc                                                                >  2:9902817/1‑150 (MQ=255)
                                                                                       ggCGACCAGGCGGTTGTTAACAACGAAGGCGAGAGCGCCATCACCAACGGTGGCACCGGCACGCAGATTAACGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGGACGGAAATTGCTGGc                                                                >  1:7922471/1‑150 (MQ=255)
                                                                                        gCGACCAGGCGGTTGTTAACAACGAAGGCGAGAGCGCCATCACCAACGGTGGCACCGGCACGCAGATTAACGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATTGCTGGCa                                                               >  1:9568343/1‑150 (MQ=255)
                                                                                          gACCAGGCGGTTGTTAACAACGAAGGCGAGAGCGCCATCACCAACGGTGGCACCGGCACGCAGATTAACGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATTGCTGGCAAt                                                             >  2:9007711/1‑150 (MQ=255)
                                                                                           aCCAGGCGGTTGTTAACAACGAAGGCGAGAGCGCCATCACCAACGGTGGCACCGGCACTCAGATTAACGGTGAAGACGCCACCGCAAACAACAACGGAAAAAACACAGTTGACGGCAAGGATTCCACCGGTACGGAAAATGGTTTCAATa                                                            >  2:3671828/1‑150 (MQ=17)
                                                                                             cAGGCGGTTGTTAACAACGAAGGCGAGAGCGCCATCACCAACGGTGGCACCGGCACGCAGATTAACGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATTGCTGGCAATAAc                                                          <  1:7321807/150‑1 (MQ=255)
                                                                                             cAGGCGGTTGTTAACAACGAAGGCGAGAGCGCCATCACCAACGGTGGCACCGGCACGCAGATTAACGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATTGCTGGCAATAAc                                                          >  2:11702891/1‑150 (MQ=255)
                                                                                             cAGGCGGTTGTTAACAACGAAGGCGAGAGCGCCATCACCAACGGTGGCACCGGCACGCAGATTAACGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATTGCTGGCAATAAc                                                          >  2:12054777/1‑150 (MQ=255)
                                                                                             cAGGCGGTTGTTAACAACGAAGGCGAGAGCGCCATCACCAACGGTGGCACCGGCACGCAGATTAACGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATTGCTGGCAATAAc                                                          <  1:1128627/150‑1 (MQ=255)
                                                                                              aGGCGGTTGTTAACAACGAAGGCGAGAGCGCCATCACCAACGGTGGCACCGGCACGCAGATTAACGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATTGCTGGCAATAACg                                                         >  2:7620731/1‑150 (MQ=255)
                                                                                                 cGGTTGTTAACAACGAAGGCGAGAGCGCCATCACCAACGGTGGCACCGGCACGCAGATTAACGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATTGCTGGCAATAACGGGa                                                      >  1:5236061/1‑150 (MQ=255)
                                                                                                  ggTTGTTAACAACGAAGGCGAGAGCGCCATCACCAACGGTGGCACCGGCACGCAGATTAACGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATTGCTGGCAATAACGGGaa                                                     <  2:5820347/150‑1 (MQ=255)
                                                                                                  catcgttAACAACGAAGGCGAGAGCGCCATCACCAACGGTGGCACCGGCACGCAGATTAACGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATTGCt                                                                   <  2:6474974/132‑1 (MQ=255)
                                                                                                   gttgttAACAACGAAGGCGAGAGCGCCATCACCAACGGTGGCACCGGCACGCAGATTAACGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATTGCTGGCAATAACGGGAAg                                                    <  1:6737415/150‑1 (MQ=255)
                                                                                                   gttgttAACAACGAAGGCGAGAGCGCCATCACCAACGGTGGCACCGGCACGCAGATTAACGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATTGCTGGCAATAACGGGAAg                                                    >  1:3286675/1‑150 (MQ=255)
                                                                                                     tgttAACAACGAAGGCGAGAGCGCCATCACCAACGGTGGCACCGGCACGCAGATTAACGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATTGCTGGCAATAACGGGAAGGt                                                  <  1:2598790/150‑1 (MQ=255)
                                                                                                      gttAACAACGAAGGCGAGAGCGCCATCACCAACGGTGGCACCGGCACGCAGATTAACGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATTGCTGGCACTAACGGGAAGGTg                                                 >  2:2553705/1‑150 (MQ=255)
                                                                                                      gttAACAACGAAGGCGAGAGCGCCATCACCAACGGTGGCACCGGCACGCAGATTAACGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATTGCTGGCAATAACGGGAAGGTg                                                 >  1:2057861/1‑150 (MQ=255)
                                                                                                       ttAACAACGAAGGCGAGAGCGCCATCACCAACGGTGGCACCGGCACGCAGATTAACGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATTGCTGGCAATAACGGGAAGGTGa                                                >  2:10576602/1‑150 (MQ=255)
                                                                                                       ttAACAACGAAGGCGAGAGCGCCATCACCAACGGTGGCACCGGCACGCAGATTAACGGTGATGACGCCACCGCGAAAAACAAAGGCAAAACCACAGTTGAAGGAAAAGATTCCACCGGTACCAAAATAGCTGGCAATAACGGCAAGGtca                                                >  2:11517131/1‑148 (MQ=17)
                                                                                                         aacaacGAAGGCGAGAGCGCCATCACCAACGGTGGCACCGGCACGCAGATTAACGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATTGCTGGCAATAACGGGAAGGTGAtt                                              >  1:8499894/1‑150 (MQ=255)
                                                                                                         aacaacGAAGGCGAGAGCCCCATCACCAACGGCGGCACCGGCACGCAGATAAACGGTCACGACGCCACCGCCAACAACACCGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACCCAAATCGCGGGCAATAGCGGCCAGGTGAtt                                              >  2:3625644/1‑150 (MQ=14)
                                                                                                          acaacGAAGGCGAGAGCGCCATCACCAACGGTGGCACCGGCACGCAGATTAACGGTGATGACGCCACGGCAAAAAACAAAGGAAAAACCACCGTTGACGGCAAGGAATACACCGGTACGGAAATAGCTGGCAATAACGGGACGGTGATTc                                             >  2:10417239/1‑150 (MQ=17)
                                                                                                          acaacGAAGGCGAGAGCGCCATCACCAACGGTGGCACCGGCACGCAGATTAACGGTGAGGACGCCACGGCAAACAACATCGGCAAAACCACCGTTGACGGCAAGGATTACACCGCTACGGAAATGGCTGGCAATAACGGGAAGGTGATTc                                             >  1:5123397/1‑150 (MQ=17)
                                                                                                            aacGAAGGCGAGAGCGCCATCACCAACGGTGGCACCGGCACGCAGATTAACGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATTGCTGGCAATAACGGGAAGGTGATTCAg                                           >  2:7936389/1‑150 (MQ=255)
                                                                                                            aacGAAGGCGAGAGCGCCATCACCAACGGTGGCACCGGCACGCAGATTAACGGTGATGACGCCACGGCAAACAACAACGACAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATTGCTGGCAATAACGGGAAGGTGATTCAg                                           >  1:11170197/1‑150 (MQ=255)
                                                                                                             acGAAGGCGAGAGCGCCATCACCAACGGTGGCACCGGCACGCAGATTAACGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACACGGCCGGAAATAGCTGGAAATAACGGGAAGGTGATTCAgg                                          >  1:3695543/1‑150 (MQ=17)
                                                                                                              cGAAGGCGAGAGCGCCATCACCAACGGTGGCACCGGCACGCAGATTAACGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATTGCTGGCAATAACGGGAAGGTGATTCAGGa                                         >  1:6559367/1‑150 (MQ=255)
                                                                                                              cGAAGGCGAGAGCGCCATCACCAACGGTGGCACCGGCACGCAGATTAACGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATTGCTGGCAATAACGGGAAGGTGATTCAGGa                                         <  2:2635558/150‑1 (MQ=255)
                                                                                                                 aGGCGAGAGCGCGATCACCATCGGTGGCACCGGCTCGCAGATTAACGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATTGCTGGCAATAACGGGAAGGTGATTCAGGACgg                                      <  1:5746111/150‑1 (MQ=255)
                                                                                                                   gCGAGAGCGCCATCACCAACGGTGGCACCGGCACGCAGATTAACGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATTGCTGGCAATAACGGGAAGGTGATTCAGGACGGCg                                    >  1:4237385/1‑150 (MQ=255)
                                                                                                                    cGAGAGCGCCATCACCAACGGTGGCACCGGCACGCAGATTAACGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATTGCTGGCAATAACGGGAAGGTGATTCAGGACGGCGa                                   >  2:692132/1‑150 (MQ=255)
                                                                                                                    cGAGAGCGCCATCACCAACGGTGGCACCGGCACGCAGATTAACGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATTGCTGGCAATAACGGGAAGGTGATTCAGGACGGCGa                                   >  1:7572330/1‑150 (MQ=255)
                                                                                                                    cGAGAGCGCCATCACCAACGGTGGCACCGGCACGCAGATTAACGGTGAGGACGCCACGGCGAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGGACGGAAATGGCGGGCAATACCGGCAAGGTGATTCAGGACGGCGa                                   >  2:4680034/1‑150 (MQ=17)
                                                                                                                       gagCACCATCACCAACGGTGGCACCGGCACCCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATTGCTGGCAATAACGGGAAGGTGATTCAGGACGGCGATCt                                <  2:3282064/150‑1 (MQ=255)
                                                                                                                        agCGCCATCACCAACGGTGGCACCGGCACGCAGATTAACGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATTGCTGGCAATAACGGGAAGGTGATTCAGGACGGCGATCTg                               <  1:10593683/150‑1 (MQ=255)
                                                                                                                        agCGCCATCACCAACGGTGGCACCGGCACGCAGATTAACGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATTGCTGGCAATAACGGGAAGGTGATTCAGGACGGCGATCTg                               >  1:6770828/1‑150 (MQ=255)
                                                                                                                         gcgcCATCACCAACGGTGGCACCGGCACGCAGATTAACGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATTGCTGGCAATAACGGGAAGGTGATTCAGGACGGCGATCTgg                              <  1:9044126/150‑1 (MQ=255)
                                                                                                                          cgcCATCACCACCGGTGGCACCGGCACGCAGATTAACGGTGATGACGCCACGGCAAACCACAACGGCAACACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATTGCTGGCAATAACGGGAAGGTGATTCAGGACGGCGATCTGGa                             <  2:2889477/150‑1 (MQ=255)
                                                                                                                          caccATCACCAACGGTGGCACCGGCACCCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATTGCTGGCAATAACGGGAAGGTGATTCAGGACGGCGATCTGGa                             >  2:3911729/3‑150 (MQ=255)
                                                                                                                           gcCATCACCAACGGTGGCACCGGCACGCAGATTAACGGTGATGACGCCACGGCAAAAAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATTGCTGGCAATAACGGGAAGGTGATTCAGGACGGCGATCTGGAt                            >  1:5400135/1‑150 (MQ=255)
                                                                                                                            ccATCACCAACGGTGGCACCGGCACGCAGATTAACGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATTGCTGGCAATAACGGGAAGGTGATTCAGGACGGCGATCTGGATg                           >  1:10363308/1‑150 (MQ=255)
                                                                                                                             cATCACCAACGGTGGCACCGGCACGCAGATTAACGGTGATGACGCCACGGCAAACAACACCGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATTGCTGGCAATAACGGGAAGGTGATTCAGGACGGCGATCTGGATGt                          >  2:5197487/1‑150 (MQ=255)
                                                                                                                             cATCACCAACGGTGGCACCGGCACGCAGATTAACGGTGATGACGCCACGGCAAACAACAACg                                                                                                                  <  1:10076754/62‑1 (MQ=255)
                                                                                                                             cATCACCAACGGTGGCACCGGCACGCAGATTAACGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATTGCTGGCAATAACGGGAAGGTGATTCAGGACGGCGATCTGGATGt                          <  1:12155310/150‑1 (MQ=255)
                                                                                                                               tCACCAACGGTGGCACCGGCACGCAGATTAACGGTGATGACGCCACGGCAAACAACAACGGCAAAaccacc                                                                                                       <  2:6314302/71‑1 (MQ=39)
                                                                                                                               tCACCAACGGTGGCACCGGCACCCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATTGCTGGCAATAACGGGAAGGTGATTCAGGACGGCGATCTGGATGTCa                        <  1:3748755/150‑1 (MQ=255)
                                                                                                                                  ccAACGGTGGCACCGGCACCCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATTGCTGGCAATAACGGGAAGGTGa                                                >  1:4075590/1‑123 (MQ=255)
                                                                                                                                  ccAACGGTGGCACCGGCACCCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATTGCTGGCAATAACGGGAAGGTGa                                                <  2:4075590/123‑1 (MQ=255)
                                                                                                                                  ccAACGGTGGCACCGGCACCCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATTGCTGGCAATAACGGGAAGGTGATTCAGGACGGCGATCTGGa                             <  2:5624149/142‑1 (MQ=255)
                                                                                                                                   cAACGGTGGCACCGGCACGCAGATTAGCGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATTGCTGGCAATAACGGGAAGGTGATTCAGGACGGCGATCTGGATGTCAgcgg                    >  1:3504522/1‑150 (MQ=255)
                                                                                                                                   cAACGGTGGCACCGGCACGCAGATTAACGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATTGCTGGCAATAACGGGAAGGTGATTCAGGACGGCGATCTGGATGTCAgcgg                    >  1:9806128/1‑150 (MQ=255)
                                                                                                                                        gTGGCACCGGCACGCAGATTAACGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATTGCTGGCAATAACGGGAAGGTGATTCAGGACGGCGATCTGGATGTCAgcggcggcg               <  2:5268085/150‑1 (MQ=255)
                                                                                                                                        gTGGCACCGGCACCCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATTGCTGGCAATAACGGGAAGGTGATTCAGGACGGCGATCTGGATGTCAgcggcggcg               <  2:6158511/150‑1 (MQ=255)
                                                                                                                                        gTGGCACCGGCACCCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATTGCTGGCAATAACGGGAAGGTGATTCAGGACGGCGATCTGGATGTCAgcggcggcg               <  1:3911729/150‑1 (MQ=255)
                                                                                                                                               cGGCACCCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATTGCTGGCAATAACGGGAAGGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCAc          >  1:302474/1‑148 (MQ=255)
                                                                                                                                                 gCACGCAGATTAACGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATTGCTGGCAATAACGGGAAGGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTa      >  1:4959343/1‑150 (MQ=255)
                                                                                                                                                   aCGCAGATTAACGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATTGCTGGCAATAACGGGAAGGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTAtt    >  2:10154392/1‑150 (MQ=255)
                                                                                                                                                    cGCAGATTAACGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATTGCTGGCAATAACGGGAAGGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTg   >  1:3604871/1‑150 (MQ=255)
                                                                                                                                                     gCAGATTAACGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATTGCTGGCAATAACGGGAAGGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGa  >  2:1666471/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
ATTGATATCACCGGCGACAGCGCAACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATCGGTATCCAGGTTGACGGCGACCAGGCGGTTGTTAACAACGAAGGCGAGAGCGCCATCACCAACGGTGGCACCGGCACGCAGATTAACGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATTGCTGGCAATAACGGGAAGGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGA  >  NC_000913/1466481‑1466779

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: