Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,720,364 G→T 8.2% intergenic (+21/+26) slyB → / ← slyA outer membrane lipoprotein/global transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,720,3640GT 8.2% 1341.1 / 31.7 414intergenic (+21/+26)slyB/slyAouter membrane lipoprotein/global transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base G (254/109);  new base T (2/49);  total (256/158)
Fisher's exact test for biased strand distribution p-value = 9.22e-21
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 441 total aligned reads displayed.

CTGGAAATTCGTAAAGACGATGGTAATACCATCATGGTGGTACAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAGTTGCTCCAGTTCCTCTGCGGAGATGCCATGTAATATTTCCGCGCGGGTTTTGTTAATAACAGCT  >  NC_000913/1720215‑1720513
                                                                                                                                                       |                                                                                                                                                     
cTGGAAATTCGTAAAGACGATGGTAATACCATCATGGTGGTACAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAACGTGTCGTACTGTCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCTTGTGGTCAgg                                                                                                                                                       >  1:4512/1‑150 (MQ=255)
 tGGAAATTCGTAAAGACGATGGTAATACCATCATGGTGGTACAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGt                                                                                                                                                      >  2:5510001/1‑150 (MQ=255)
  ggAAATTCGTAAAGACGATGGTAATACCATCATGGTGGTACAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGtt                                                                                                                                                     >  1:3877507/1‑150 (MQ=255)
     aaTTCGTAAAGACGATGGTAATACCATCATGGTGGTACAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACt                                                                                                                                                  <  2:7815014/150‑1 (MQ=255)
       ttCGTAAAGACGATGGTAATACCATCATGGTGGTACAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGa                                                                                                                                                >  2:3639597/1‑150 (MQ=255)
       ttCGTAAAGACGATGGTAATACCATCATGGTGGTACAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGa                                                                                                                                                <  1:10815467/150‑1 (MQ=255)
        tCGTCAAGACGATGGTAATACCATCATGGTGGTACAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGAc                                                                                                                                               <  1:11345664/150‑1 (MQ=255)
        tCGTAAAGACGATGGTAATACCATCATGGTGGTACAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGAc                                                                                                                                               <  1:3592383/150‑1 (MQ=255)
        tCGTAAAGACGATGGTAATACCATCATGGTGGTACAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGAc                                                                                                                                               <  1:6706741/150‑1 (MQ=255)
         cGTAAAGACGATGGTAATACCATCATGGTGGTACAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGAcc                                                                                                                                              <  1:2217201/150‑1 (MQ=255)
           tAAAGACGATGGTAATACCATCATGGTGGTACAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACcac                                                                                                                                            >  1:9945944/1‑150 (MQ=255)
           tAAAGACGATGGTAATACCATCATGGTGGTACAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACcac                                                                                                                                            >  1:3314800/1‑150 (MQ=255)
           gaaaGACGATGGTACGACCATCATGGTGGTACAGAAACAAGGCAACACTCGGTTCTCTCCGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTGGCGTGTGGTCAGGTTACTGACcac                                                                                                                                            <  2:4542824/149‑1 (MQ=255)
            aaaGACGATGGTAATACCATCATGGTGGTACAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGTAACCTGACcaca                                                                                                                                           <  2:6869146/150‑1 (MQ=255)
            aaaGACGATGGTAATACAATCATGGTGGTACAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGTAACCTGACcaca                                                                                                                                           <  1:7013907/150‑1 (MQ=255)
                   aTGGTAATACCATCATGGTGGTACAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACACGccccc                                                                                                                                    >  1:8301157/1‑150 (MQ=255)
                     ggTAATACCATCATGGTGGTACAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCtt                                                                                                                                  >  2:3434969/1‑150 (MQ=255)
                           aCCATCATGGTGGTACAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTc                                                                                                                            >  2:6515897/1‑150 (MQ=255)
                             catcatGGTGGTACAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCAc                                                                                                                          >  1:8257571/1‑150 (MQ=255)
                             catcatGGTGGTACAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCAc                                                                                                                          >  2:816109/1‑150 (MQ=255)
                             catcatGGTGGTACAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCAc                                                                                                                          >  2:712110/1‑150 (MQ=255)
                                  tggtggTACTGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCttt                                                                                                                     >  1:6866188/1‑150 (MQ=255)
                                    gtggtACAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTgg                                                                                                                   >  1:957219/1‑150 (MQ=255)
                                     tggtACTGAAACATGGAAACACTCGTTTCTCTCGGGGCAAAGGTGTCGTACTGGCCTGCAATGGCTGTCTGGTGACGGTTTCTCCGCGATAATTATGTTTGCGTGTGGTCTGGTTCCGGACCACACGCCCCCTTCATTTCACCCTTTGGc                                                                                                                  <  2:4142001/150‑1 (MQ=255)
                                     tggtACATAACCACGGCACCACTGGTTTGTCTCGGGGCCAAGGGGACGTCGTGGCCAGCACTGGCAGTAGGGTGACCGTTTCTCCGCGCTAATTATTTTTGCGTGTGGTCTGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGc                                                                                                                  <  2:4983434/150‑1 (MQ=255)
                                     tggtACAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGc                                                                                                                  >  2:8489654/1‑150 (MQ=255)
                                     tggtACAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGGTACTGACCACACGCCCCCTTCATTTCACCCTTTGGc                                                                                                                  >  1:7467954/1‑150 (MQ=255)
                                        tACAGAAACATGGCAACACTGGTTTCTCTCCGGGCCACCTTGTCGTCCTGGCCAGCATGGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATACGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACACTTTGGCCTg                                                                                                               <  2:12181191/150‑1 (MQ=255)
                                        tACAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTg                                                                                                               >  1:7145003/1‑150 (MQ=255)
                                        tACAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTCGGCCTg                                                                                                               >  2:480433/1‑150 (MQ=255)
                                         aCAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGt                                                                                                              >  1:5041098/1‑150 (MQ=255)
                                         aCAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGt                                                                                                              >  2:2109333/1‑150 (MQ=255)
                                           aGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTaa                                                                                                            >  1:2743486/1‑150 (MQ=255)
                                           aGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTaa                                                                                                            >  1:2538938/1‑150 (MQ=255)
                                                  aGGCAACACTCGTTTCTCTCCGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCTTTTCTCCGCTCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATg                                                                                                     <  2:10131665/150‑1 (MQ=255)
                                                    gCAACACTCGTTTCTCTCCGGGCCAACGTGTCGTACGGGCCAGCAGTGGCAGTCAGGTGAGCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCCGGTTACTGAGGTCACGCCCGCGGCGTGTCACCCTTTGGCCTGTTACTCAATggt                                                                                                   >  2:3217832/1‑148 (MQ=255)
                                                     cAACACTCGTTTCTCTCCGGGCCAACGTGTCTTACGGGACAGCACTGGCTGTCTGTTGACGGTTTCTCCGCGATAAATAAGTTTGCGTGTGGTCTGTTTATTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGata                                                                                                  <  2:2846902/150‑1 (MQ=255)
                                                           tCGTTTCTCTCCGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTACACCCTTTGGCCTGTAACTCAATGATATTATGc                                                                                            >  1:1649738/1‑150 (MQ=255)
                                                           tCGTTTCTCTCCGGGACAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTATGGCCTGTAACTCAATGATATTATGc                                                                                            >  2:12274439/1‑150 (MQ=255)
                                                             gTTTCTCTCCGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTc                                                                                          >  1:10315680/1‑150 (MQ=255)
                                                             gTTTCTCTCCGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTc                                                                                          >  1:3105891/1‑150 (MQ=255)
                                                                  tctcCGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGtt                                                                                     >  2:394666/1‑150 (MQ=255)
                                                                  tctcCGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGtt                                                                                     >  1:1546579/1‑150 (MQ=255)
                                                                    tcCGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGtttt                                                                                   >  1:4555746/1‑150 (MQ=255)
                                                                    tcCGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGtttt                                                                                   >  1:10019508/1‑150 (MQ=255)
                                                                    tcCGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGtttt                                                                                   >  1:8194004/1‑150 (MQ=255)
                                                                     ccGGGCAACCGTGTCGTACTGGCCAGCATTGGCAGTCTGGTGACCGTTTCTCCGCGCTAAATATGTTTGCGTGTGGTCTGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTg                                                                                  <  2:6198665/150‑1 (MQ=255)
                                                                        ggCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGa                                                                               >  2:922890/1‑150 (MQ=255)
                                                                         gCCAACGTGTCGTACTGGCCAGAATGGGACTTCCGGTGACCGTTTCTCCGCGCTAAATATTTTTGCGTGTGGTCTGTTTACTCACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGAt                                                                              <  2:6729796/150‑1 (MQ=255)
                                                                             aCGTGTCGTACGGGCCTGAACTGGCTGTCAGGTGACCTTTGCACCGCGCTAAATATTTTTGGTTTGGTTCCGGTTCGGGACCCCACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGc                                                                          <  2:2669952/150‑1 (MQ=255)
                                                                               gtgtCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGt                                                                        >  2:1557699/1‑150 (MQ=255)
                                                                                  tCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAAt                                                                     >  2:5818611/1‑150 (MQ=255)
                                                                                  tCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATGTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAAt                                                                     >  2:172039/1‑150 (MQ=255)
                                                                                     tACTTGCCCGCAATGGAAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTATGCGTGAGGTCAGGTAACTGACCACACGCCCCCTTCATTTCAAACTTTGGCCTGTAACTCAATGATATAATGCTCAAGGTTTGCGATGATCGTAATCAg                                                                  >  2:10443325/1‑150 (MQ=255)
                                                                                      cgtGGCCAGCTTGGGCTGTCTGGTGACCGTTACTCCGCGATAATTTTGTTTGGTTGTGGTCTGTTTACTCACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAGt                                                                 <  1:11156528/148‑1 (MQ=255)
                                                                                      aCTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAGt                                                                 >  1:9477581/1‑150 (MQ=255)
                                                                                      aCTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAGt                                                                 >  2:1834047/1‑150 (MQ=255)
                                                                                        tGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATACGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTGCATTTCACCCTTTGGCCTGTCACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAGTTg                                                               >  2:4315425/1‑150 (MQ=255)
                                                                                        tGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAGTTg                                                               >  1:6264845/1‑150 (MQ=255)
                                                                                        tGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAGTTg                                                               >  2:2237423/1‑150 (MQ=255)
                                                                                           ccAGCATTGGCTGTCTGGTGACCGTTTCTCCGCTATAATTAAGTTTGGTTTTGGTCTGTTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAGTTGCTc                                                            <  2:7461946/150‑1 (MQ=255)
                                                                                            cAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAGTTGCTcc                                                           >  2:11815890/1‑150 (MQ=255)
                                                                                             aGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAGTTGCTCCa                                                          >  2:7972272/1‑150 (MQ=255)
                                                                                                aagggCTGTCTGGTGCCCGTTTCTCCGCGCAAAATACTTTTGCGTGTGTTCTGTTTACTCACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAGTTGCTCCAGtt                                                       <  1:10973345/147‑1 (MQ=255)
                                                                                                 ttGGCTGTCTGGTGACCGTTTCTCCGCTCTAAATATTTTTGGTTGTGTTATGTTTACGGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAGTTGCTCCAGTTc                                                      <  2:10735676/149‑1 (MQ=255)
                                                                                                    gCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAGTTGCTCCAGTTCctc                                                   >  2:5190297/1‑150 (MQ=255)
                                                                                                      aGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAGTTGCTCCAGTTCCTCTg                                                 >  1:8648855/1‑150 (MQ=255)
                                                                                                           tggTGTCCTTTTATCGGCTTTAAATTAGTTTGCTTGTGGTCAGTTTACTTACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAGTTGCTCCAGTTCCTCTGCGga                                             <  1:9205006/149‑1 (MQ=255)
                                                                                                            tttGAGCGTTTGTCCGCGATAAATATGTTGGGGTGTGTTCTGGTTACTGACCCCACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAGTTGCTCCAGTTCCTCTGCGgag                                            <  2:7243834/148‑1 (MQ=255)
                                                                                                            ggTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAGTTGCTCCAGTTCCTCTGCGgag                                            >  2:11295673/1‑150 (MQ=255)
                                                                                                             gTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAGTTGCTCCAGTTCCTCTGCGgaga                                           >  1:1918692/1‑150 (MQ=255)
                                                                                                              tgccgttttCTCCGCGATAAATATGTTTGGGTGTGGTCTGTTTACTCACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAGTTGCTCCAGTTCCTCTGCGGAGAt                                          <  1:12263773/144‑1 (MQ=255)
                                                                                                              tGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAGTTGCTCCAGTTCCTCTGCGGAGAt                                          <  2:10908452/150‑1 (MQ=255)
                                                                                                              tGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAGTTGCTCCAGTTCCTCTGCGGAGAt                                          >  2:1434242/1‑150 (MQ=255)
                                                                                                                aCCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCGTTGGCCTGTAACTAAATTATATTATTCTGAATTTTTGAGATTAGCGTAATAAGTTGCTCCAGTTCCTCTGAGGAGAttc                                        >  1:5401854/1‑148 (MQ=255)
                                                                                                                aCCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCGTCCTTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAGTTGCTCCAGTTCCTCTGCGGAGATGc                                        >  1:9901108/1‑150 (MQ=255)
                                                                                                                 ccGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAGTTGCTCCAGTTCCTCTGCGGAGATGcc                                       >  2:11110364/1‑150 (MQ=255)
                                                                                                                        tcCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAGTTGCTCCAGTTCCTCTGCGGAGATGCCATGTAat                                >  2:8614658/1‑150 (MQ=255)
                                                                                                                          cgcgCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAGTTGCTCCAGTTCCTCTGCGGAGCTGCCATGTAatat                              >  1:7687706/1‑150 (MQ=255)
                                                                                                                            cgcTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCGGTAACACAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAGTTGCTCCAGGTCCTCTGCGGAGATGCCATGGAATAttt                            >  1:884418/1‑150 (MQ=255)
                                                                                                                            cgcTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCATCATTTCACCCTTTGGCCTGTAACACAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAGTTGCTCCAGTTCCTCTGCGGAGATGCCCGGTAATAttt                            >  1:1374593/1‑150 (MQ=255)
                                                                                                                             gcTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACACCCCCCCTTCATTTCACCCTTTGGCCAGTAACACAATGATATTATGCTCAAGTTTTGCGAAGAGCCTAATCAGTTGCTCCAGTTGCGATGCGGAGATGCCATGTAATATTTc                           >  1:11829819/1‑150 (MQ=255)
                                                                                                                               tAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACTGGCCCCCTTGATTTCACCCTTTGGGGTGTTACTCAATGATTTTATGGTGTTGTTTTGGGATGTGGGTTTTGTGTTGGTGCAGTTGGTGTGCGGTGTTGGGATGTATTAtttggg                         >  2:8273098/1‑147 (MQ=255)
                                                                                                                                         tGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAGTTGCTCCAGTTCCTCTGCGGAGATGCCATGTAATATTTCCGCGCGGGtttt               <  2:9016141/150‑1 (MQ=255)
                                                                                                                                           cGTGTGGTCTGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAGTTGCTCCAGTTCCTCTGCGGAGATGCCATGTAATATTTCCGCGCGGGTTTTGt             <  1:6096318/150‑1 (MQ=255)
                                                                                                                                            gtgtGGTCTGTTTCCTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAGTTGCTCCAGTTCCTCTGCGGAGATGCCATGTAATATTTCCGCGCGGGTTTTGtt            <  1:1154164/150‑1 (MQ=255)
                                                                                                                                            gtgtGGTCAGTAACCTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTGTGCGATGAGCGTAATCAGTTGCTCCAGTTCCTCTGCGGAGATGCCAGGTACTAGTTCCGCGCGGGTTTTGtc            >  2:2958279/1‑149 (MQ=255)
                                                                                                                                            gtgtGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCACTGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAGTTGCTCCAGGTCCTCTGCGGAGATGCCATGTAATCTGTCCGCGCGGGTTTTGtt            >  2:9825538/1‑150 (MQ=255)
                                                                                                                                            gtgtGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAGTTGCTCCAGTTCCTCTGCGGAGATGCCATGTCATATTTCCGCGCGGGTTTTGtt            >  2:5631902/1‑150 (MQ=255)
                                                                                                                                              gtgGTCAGTTTACTGCCCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAGTTGCTCCAGTTCCTCTGCGGAGATGCCATGTAATATTTCCGCGCGGGTTTTGTtaa          <  2:1683487/150‑1 (MQ=255)
                                                                                                                                               tgGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAGTTGCTCCAGTTCCTCTGCGGAGATGCCATGTAATATTTCCGCGCGGGTTTTGTtaat         >  1:5708617/1‑150 (MQ=255)
                                                                                                                                                 gTCTGGTTACTTACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAGTTGCTCCAGTTCCTCTGCGGAGATGCCATGTAATATTTCCGCGCGGGTTTTGTtaataa       <  2:2346657/150‑1 (MQ=255)
                                                                                                                                                 gTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAGTTGCTCCAGTTCCTCTGCGGAGATGCCATGTAATATTTCCGCGCGGGTTTTGTtaataa       >  2:9643432/1‑150 (MQ=255)
                                                                                                                                                   tctggTTCCTGCCCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAGTTGCTCCAGTTCCTCTGCGGAGATGCCATGTAATATTTCCGCGCGGGTTTTGTTAATAAc      <  1:11679049/147‑1 (MQ=255)
                                                                                                                                                   tCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAGTTGCTCCAGTTCCTCTGCGGAGATGCCATGTAATATTTCCGCGCGGGTTTTGTTAATAAc      >  1:5914984/1‑150 (MQ=255)
                                                                                                                                                   tCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAGTTGCTCCAGTTCCTCTGCGGAGATGCCATGTAATATTTCCGCGCGGGTTTTGTTAATAAc      >  2:11487789/1‑150 (MQ=255)
                                                                                                                                                     cggTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAGTTGCTCCAGTTCCTCTGCGGAGATGCCATGTAATATTTCCGCGCGGGTTTTGTTAATAACAg    <  1:8044551/149‑1 (MQ=255)
                                                                                                                                                       gTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAGTTGCTCCAGTTCCTCTGCGGAGATGCCATGTAATATTTCCGCGCGGGTTTTGTTAATAACAGCt  >  1:119454/1‑150 (MQ=255)
                                                                                                                                                       gTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAGTTGCTCCAGTTCCTCTGCGGAGATGCCATGTAATATTTCCGCGCGGGTTTTGTTAATAACAGCt  >  2:8837017/1‑150 (MQ=255)
                                                                                                                                                       gTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAGTTGCTCCAGTTCCTCTGCGGAGATGCCATGTAATATTTCCGCGCGGGTTTTGTTAATAACAGCt  >  2:1990674/1‑150 (MQ=255)
                                                                                                                                                       |                                                                                                                                                     
CTGGAAATTCGTAAAGACGATGGTAATACCATCATGGTGGTACAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAGTTGCTCCAGTTCCTCTGCGGAGATGCCATGTAATATTTCCGCGCGGGTTTTGTTAATAACAGCT  >  NC_000913/1720215‑1720513

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: