BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	1,876,923	C→T	5.2%	C5C (TGC→TGT)	yeaO →	DUF488 family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,876,923	0	C	T	5.2%	1982.1 / 59.9	575	C5C (TGC→TGT)	yeaO	DUF488 family protein
Reads supporting (aligned to +/- strand): ref base C (276/266); new base T (9/21); total (288/287)
Fisher's exact test for biased strand distribution p-value = 3.75e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.67e-01

Only 100 of 587 total aligned reads displayed.

CCACTTATGGGTGTTGCCATACTTTCACTACTGATGGCGATTTTCGGACTTTGCGCCGGGAGAGACAAAGAAATTCGCTAATATCCGGTGCTATAGTGACGTAACAAATCATGCGTGAAAGGGAGAACAAACACGATGAATATTCAGTGCAAACGCGTTTATGATCCGGCTGAACAGAGCGATGGTTATCGCATACTGGTCGACCGCCTCTGGCCGCGCGGTATCAAAAAAACCGATTTAGCCCTTGATGAGTGGGATAAAGAAATCACGCCGTCAACGGAACTGCGCAAAGCCTTTCA  >  NC_000913/1876774‑1877072

ccACTTATGGGTGTTGCCATACTTTCACTACTGATGGCGATTTTCGGACTTTGCGCCGGGAGAGACAAAGAAATTCGCTAATATCCGGTGCTATAGTGACGTAACAAATCATGCGTGAAAGGGAGAACAAACACGATGAATATTCAGTGc                                                                                                                                                       >  2:10831733/1‑150 (MQ=255)

ccACTTATGGGTGTTGCCATACTTTCACTACTGATGGCGATTTTCGGACTTTGCGCCGGGAGAGACAAAGAAATTCGCTAATATCCGGTGCTATAGTGACGTAACAAATCATGCGTGAAAGGGAGAACAAACACGATGAATATTCAGTGc                                                                                                                                                       >  2:12148735/1‑150 (MQ=255)

 cACTTATGGGTGTTGCCATACTTTCACTACTGATGGCGATTTTCGGACTTTGCGCCGGGAGAGACAAAGAAATTCGCTAATATCCGGTGCTATAGTGACGTAACAAATCATGCGTGAAAGGGAGAACAAACACGATGAATATTCAGTGCa                                                                                                                                                      <  1:9591392/150‑1 (MQ=255)

    ttATGGGTGTTGCCATACTTTCACTACTGATGGCGATTTTCGGACTTTGCGCCGGGAGAGACAAAGAAATTCGCTAATATCCGGTGCTATAGTGACGTAACAAATCATGCGTGAAAGGGAGAACAAACACGATGAATATTCAGTGCAAAc                                                                                                                                                   >  2:11731467/1‑150 (MQ=255)

    ttATGGGTGTTGCCATACTTTCACTACTGATGGCGATTTTCGGACTTTGCGCCGGGAGAGACAAAGAAATTCGCTAATATCCGGTGCTATAGTGACGTAACAAATCATGCGTGAAAGGGAGAACAAACACGATGAATATTCAGTGCAAAc                                                                                                                                                   <  1:3371805/150‑1 (MQ=255)

    ttATGGGTGTTGCCATACTTTCACTACTGATGGCGATTTTCGGACTTTGCGCCGGGAGAGACAAAGAAATTCGCTAATATCCGGTGCTATAGTGACGTAACAAATCATGCGTGAAAGGGAGAACAAACACGATGAATATTCAGTGCAAAc                                                                                                                                                   >  2:10403952/1‑150 (MQ=255)

      aTGGGTGTTGCCATACTTTCACTACTGATGGCGATTTTCGGACTTTGCGCCGGGAGAGACAAAGAAATTCGCTAATATCCGGTGCTATAGTGACGTAACAAATCATGCGTGAAAGGGAGAACAAACACGATGAATATTCAGTGCAAAcgc                                                                                                                                                 <  2:3641823/150‑1 (MQ=255)

      aTGGGTGTTGCCAGACTTTCACTACTGATGGCGATTTTCGGACTTTGCGCCGGGAGAGACAAGGAACTTCGCTAATATCCGGTGCTATAGTGACGTAACAAATCATGCGTGCCAGGGAGAACAAACACGAGGAATATTCAGTGCAACcgc                                                                                                                                                 >  1:5762413/1‑150 (MQ=255)

         ggTGTTGCCATACTTTCACTACTGATGGCGATTTTCGGACTTTGCGCCGGGAGAGACAAAGAAATTCGCTAATATCCGGTGCTATAGTGACGTAACAAATCATGCGTGAAAGGGAGAACAAACACGATGAATATTCAGTGCAAACGCGtt                                                                                                                                              <  1:2497495/150‑1 (MQ=255)

          gtgtTGCCATACTTTCACTACTGATGGCGATTTTCGGACTTTGCGCCGGGAGAGACAAAGAAATTCGCTAATATCCGGTGCTATAGTGACGTAACAAATCATGCGTGAAAGGGAGAACAAACACGATGAATATTCAGTGCAAACGCGttt                                                                                                                                             >  2:4807202/1‑150 (MQ=255)

           agtTGCCATACTTTCAAAACAGATGTCGATTTTAGGACTTTGAGCCGGGAGATACAAAGAAAATCGCTAATATCCGGTGCTATAGTGACGTAACAAATCATTCGTGAAAGGGAGAACAAACACGATGAATATTCAGTGAAAACGCGTTTa                                                                                                                                            >  2:11993277/2‑150 (MQ=255)

             ttGCCATACTTTCACTACTGATGGCGATTTTCGGACTTTGCGCCGGGAGAGACAAAGAAATTCGCTAATATCCGGTGCTATAGTGACGTAACAAATCATGCGTGAAAGGGAGAACAAACACGATGAATATTCAGTGCAAACGCGTTTATg                                                                                                                                          <  1:11568299/150‑1 (MQ=255)

                 cATACTTTCACTACTGATGGCGATTTTCGGACTTTGCGCCGGGAGAGACAAAGAAATTCGCTAATATCCGGTGCTATAGTGACGTAACAAATCATGCGTGAAAGGGAGAACAAACACGATGAATATTCAGTGCAAACGCGTTTATGATcc                                                                                                                                      >  1:3096245/1‑150 (MQ=255)

                 cATACTTTCACTACTGATGGCGATTTTCGGACTTTGCGCCGGGAGAGACAAAGAAATTCGCTAATATCCGGTGCTATAGTGACGTAACAAATCATGCGTGAAAGGGAGAACAAACACGATGAATATTCAGTGCAAACGCGTTTATGATcc                                                                                                                                      >  1:8701015/1‑150 (MQ=255)

                  aTACTTTCACTACTGATGGCGATTTTCGGACTTTGCGCCGGGAGAGACAAAGAAATTCGCTAATATCCGGTGCTATAGTGACGTAACAAATCATGCGTGAAAGGGAGAACAAACACGATGAATATTCAGTGCAAACGCGTTTATGATCCg                                                                                                                                     <  1:6541906/150‑1 (MQ=255)

                   tACTTTCACTACTGATGGCGATTTTCGGACTTTGCGCCGGGAGAGACAAAGAAATTCGCTAATATCCGGTGCTATAGTGACGTAACAAATCATGCGTGAAAGGGAGAACAAACACGATGAATATTCAGTGCAAACGCGTTTATGATCCgg                                                                                                                                    >  1:9013207/1‑150 (MQ=255)

                        tCACTACTGATGGCGATTTTCGGACTTTGCGCCGGGAGAGACAAAGAAATTCGCTAATATCCGGTGCTATAGTGACGTAACAAATCATGCGTGAAAGGGAGAACAAACACGATGAATATTCAGTGCAAACGCGTTTATGATCCGGCTGaa                                                                                                                               <  2:595900/150‑1 (MQ=255)

                        tCACTACTGATGGCGATTTTCGGACTTTGCGCCGGGAGAGACAAAGAAATTCGCTAATATCCGGTGCTATAGTGACGTAACAAATCATGCGTGAAAGGGAGAACAAACACGATGAATATTCAGTGCAAACGCGTTTATGATCCGGCTGaa                                                                                                                               >  2:8875325/1‑150 (MQ=255)

                          cctactGATGGCGATTTTCGGACTTTGCGCCGGGAGAGACAAAGAAATTCGCTAATATCCGGTGCTATAGTGACGTAACAAATCATGCGTGAAAGGGAGAACAAACACGATGAATATTCAGTGCAAACGCGTTTATGATCCGGCTGAACa                                                                                                                             >  2:10978527/2‑150 (MQ=255)

                            tactGATGGCGATTTTCGGACTTTGCGCCGGGAGAGACAAAGAAATTCGCTAATATCCGGTGCTATAGTGACGTAACAAATCATGCGTGAAAGGGAGAACAAACACGATGAATATTCAGTGCAAACGCGTTTATGATCCGGCTGAACaga                                                                                                                           <  2:9026151/150‑1 (MQ=255)

                               tGATGGCGATTTTCGGACTTTGCGCCGGGAGAGACAAAGAAATTCGCTAATATCCGGTGCTATAGTGACGTAACAAATCATGCGTGAAAGGGAGAACAAACACGATGAATATTCAGTGCAAACGCGTTTATGATCCGGCTGAACAGAGCg                                                                                                                        >  1:2013260/1‑150 (MQ=255)

                                 aTGGCGATTTTCGGACTTTGCGCCGGGAGAGACAAAGAAATTCGCTAATATCCGGTGCTATAGTGACGTAACAAATCATGCGTGAAAGGGAGAACAAACACGATGAATATTCAGTGCAAACGCGTTTATGATCCGGCTGAACAGAGCGAt                                                                                                                      <  1:1931259/150‑1 (MQ=255)

                                 aTGGCGATTTTCGGACTTTGCGCCGGGAGAGACAAAGAAATTCGCTAATATCCGGTGCTATAGTGACGTAACAAATCATGCGTGAAAGGGAGAACAAACACGATGAATATTCAGTGCAAACGCGTTTATGATCCGGCTGAACAGAGCGAt                                                                                                                      >  2:4099477/1‑150 (MQ=255)

                                  tGGCGATTTTCGGACTTTGCGCCGGGAGAGACAAAGAAATTCGCTAATATCCGGTGCTATAGTGACGTAACAAATCATGCGTGAAAGGGAGAACAAACACGATGAATATTCAGTGCACACGCGTTTATGATCCGGCTGAACAGAGCGATg                                                                                                                     >  1:9967420/1‑150 (MQ=255)

                                  tGGCGATTTTCGGACTTTGCGCCGGGAGAGACAAAGAAATTCGCTAATATCCGGTGCTATAGTGACGTAACAAATCATGCGTGAAAGGGAGAACAAACACGATGAATATTCAGTGCAAACGCGTTTATGATCCGGCTGAACAGAGCGATg                                                                                                                     <  1:10353581/150‑1 (MQ=255)

                                      gATTTTCGGACTTTGCGCCGGGAGAGACAAAGAAATTCGCTAATATCCGGTGCTATAGTGACGTAACAAATCATGCGTGAAAGGGAGAACAAACACGATGAATATTCAGTGCAAACGCGTTTATGATCCGGCTGAACAGAGCGATGGTTa                                                                                                                 >  2:10232533/1‑150 (MQ=255)

                                      gATTTTCGGACTTTGCGCCGGGAGAGACAAAGAAATTCGCTAATATCCGGTGCTATAGTGACGTAACAAATCATGCGTGAAAGGGAGAACAAACACGATGAATATTCAGTGCAAACGCGTTTATGATCCGGCTGAACAGAGCGATGGTTa                                                                                                                 >  1:11192894/1‑150 (MQ=255)

                                         tttCGGACTTTGCGCCGGGAGAGACAAAGAAATTCGCTAATATCCGGTGCTATAGTGACGTAACAAATCATGCGTGAAAGGGAGAACAAACACGATGAATATTCAGTGCAAACGCGTTTATGATCCGGCTGAACAGAGCGATGGTTATCg                                                                                                              <  1:9242232/150‑1 (MQ=255)

                                          ttCGGACTTTGCGCCGGGAGAGACAAAGAAATTCGCTAATATCCGGTGCTATAGTGACGTAACAAATCATGCGTGAAAGGGAGAACAAACACGATGAATATTCAGTGCAAACGCGTTTATGATCCGGCTGAACAGAGCGATGGTTATCGc                                                                                                             <  2:10567327/150‑1 (MQ=255)

                                          ttCGGACTTTGCGCCGGGAGAGACAAAGAAATTCGCTAATATCCGGTGCTATAGTGACGTAACAAATCATGCGTGAAAGGGAGAACAAACACGATGAATATTCAGTGCAAACGCGTTTATGATCCGGCTGAACAGAGCGATGGTTATCGc                                                                                                             >  1:2670688/1‑150 (MQ=255)

                                          ttCGGACTTTGCGCCGGGAGAGACAAAGAAATTCGCTAATATCCGGTGCTATAGTGACGTAACAAATCATGCGTGAAAGGGAGAACAAACACGATGAATATTCAGTGCAAACGCGTTTATGATCCGGCTGAACAGAGCGATGGTTATCGc                                                                                                             <  1:10102461/150‑1 (MQ=255)

                                             ggACTTTGCGCCGGGAGAGACAAAGAAATTCGCTAATATCCGGTGCTATAGTGACGTAACAAATCATGCGTGAAAGGGAGAACAAACACGATGAATATTCAGTGCAAACGCGTTTATGATCCGGCTGAACAGAGCGATGGTTATCGCATa                                                                                                          >  1:5517136/1‑150 (MQ=255)

                                              gACTTTGCGCCGGGAGAGACAAAGAAATTCGCTAATATCCGGTGCTATAGTGACGTAACAAATCATGCGTGAAAGGGAGAACAAACACGATGAATATTCAGTGCAAACGCGTTTATGATCCGGCTGAACAGAGCGATGGTTATCGCATAc                                                                                                         <  1:1640096/150‑1 (MQ=255)

                                              gACTTTGCGCCGGGAGAGACAAAGAAATTCGCTAATATCCGGTGCTATAGTGACGTAACAAATCATGCGTGAAAGGGAGAACAAACACGATGAATATTCAGTGCAAACGCGTTTATGATCCGGCTGAACAGAGCGATGGTTATCGCATAc                                                                                                         >  2:2767237/1‑150 (MQ=255)

                                                cTTTGCGCCGGGAGAGACAAAGAAATTCGCTAATATCCGGTGCTATAGTGACGTAACAAATCATGCGTGAAAGGGAGAACAAACACGATGAATATTCAGTGCAAACGCGTTTATGATCCGGCTGAACAGAGCGATGGTTATCGCATACTg                                                                                                       <  1:7961590/150‑1 (MQ=255)

                                                   tGCGCCGGGAGAGACAAAGAAATTCGCTAATATCCGGTGCTATAGTGACGTAACAAATCATGCGTGAAAGGGAGAACAAACACGATGAATATTCAGTGTAAACGCGTTTATGATCCGGCTGAACAGAGCGATGGTTATCGCATACTGGTc                                                                                                    <  1:8326242/150‑1 (MQ=255)

                                                   tGCGCCGGGAGAGACAAAGAAATTCGCTAATATCCGGTGCTATAGTGACGTAACAAATCATGCGTGAAAGGGAGAACAAACACGATGAATATTCAGTGCAAACGCGTTTATGATCCGGCTGAACAGAGCGATGGTTATCGCATACTGGTc                                                                                                    <  2:861731/150‑1 (MQ=255)

                                                      gcCGGGAGAGACAAAGAAATACGCTAAAATCCGGTGCTATAGTGACGTAACAAATCATGCGTGAAAGGGAGAACAAACACGATGAATATTCAGTGCAAACGCGTTTATGATCCGGCTGAACAGAGCGATGGTTATCGCATACTGGTCGAc                                                                                                 >  1:9357162/1‑150 (MQ=255)

                                                         gggAGAGACAAAGAAATTCGCTAATATCCGGTGCTATAGTGACGTAACAAATCATGCGTGAAAGGGAGAACAAACACGATGAATATTCAGTGCAAACGCGTTTATGATCCGGCTGAACAGAGCGATGGTTATCGCATACTGGTCGACCGc                                                                                              <  2:2087789/150‑1 (MQ=255)

                                                           gagagaCAAAGAAATTCGCTAATATCCGGTGCTATAGTGACGTAACAAATCATGCGTGAAAGGGAGAACAAACACGATGAATATTCAGTGCAAACGCGTTTATGATCCGGCTGAACAGAGCGATGGTTATCGCATACTGGTCGACCGCct                                                                                            <  2:5585573/150‑1 (MQ=255)

                                                           gagagaCAAAGAAATTCGCTAATATCCGGTGCTATAGTGACGTAACAAATCATGCGTGAAAGGGAGAACAAACACGATGAATATTCAGTGCAAACGCGTATATGATCCGGCTGAACAGAGCGATGGTTATCGCATACTGGTCGACCGCct                                                                                            >  1:11356589/1‑150 (MQ=255)

                                                             gagaCAAAGAAATTCGCTAATATCCGGTGCTATAGTGACGTAACAAATCATGCGTGAAAGGGAGAACAAACACGATGAATATTCAGTGCAAACGCGTTTATGATCCGGCTGAACAGAGCGATGGTTATCGCATACTGGTCGACCGCctct                                                                                          >  2:9386930/1‑150 (MQ=255)

                                                               gaCAAAGAACTTCGCTAATATCCGGTGCTATAGTGACGTAACAAATCATGCGTGAAAGGGAGAACAAACACGATGAATATTCAGTGCCAACGCGTTTATGATCCGGCTGAACAGAGCGATGGTTATCGCATACTGGTCGACCGCCTCTgg                                                                                        <  2:7151613/150‑1 (MQ=255)

                                                               gaCAAAGAAATTCGCTAATATCCGGTGCTATAGTGACGTAACAAATCATGCGTGAAAGGGAGAACAAACACGATGAATATTCAGTGCAAACGCGTTTATGATCCGGCTGAACAGAGCGATGGTTATCGCATACTGGTCGACCGCCTCTgg                                                                                        >  1:6421007/1‑150 (MQ=255)

                                                               gaCAAAGAAATTCGCTAATATCCGGTGCTATAGTGACGTAACAAATCATGCGTGAAAGGGAGAACAAACACGATGAATATTCAGTGCAAACGCGTTTATGATCCGGCTGAACAGAGCGATGGTTATCGCATACTGGTCGACCGCCTCTgg                                                                                        <  2:4917111/150‑1 (MQ=255)

                                                                  aaaGAAATTCGCTAATATCCGGTGCTATAGTGACGTAACAAATCATGCGTGAAAGGGAGAACAAACACGATGAATATTCAGTGTAAACGCGTTTATGATCCGGCTGAACAGAGCGATGGTTATCGCATACTGGTCGACCGCCTCTGGCcg                                                                                     >  1:2433968/1‑150 (MQ=255)

                                                                  aaaGAAATTCGCTAATATCCGGTGCTATAGTGACGTAACAAATCATGCGTGAAAGGGAGAACAAACACGATGAATATTCAGTGCAAACGCGTTTATGATCCGGCTGAACAGAGCGATGGTTATCGCATACTGGTCGACCGCCTCTGGCcg                                                                                     >  2:8720998/1‑150 (MQ=255)

                                                                     gAAATTCGCTAATATCCGGTGCTATAGTGACGTAACAAATCATGCGTGAAAGGGAGAACAAACACGATGAATATTCAGTGCAAACGCGTTTATGATCCGGCTGAACAGAGCGATGGTTATCGCATACTGGTCGACCGCCTCTGGCcgcgc                                                                                  <  1:6002502/150‑1 (MQ=255)

                                                                     gAAATTCGCTAATATCCGGTGCTATAGTGACGTAACAAATCATGCGTGAAAGGGAGAACAAACACGATGAATATTCAGTGCAAACGCGTTTATGATCCGGCTGAACAGAGCGATGGTTATCGCATACTGGTCGACCGCCTCTGGCcgcgc                                                                                  <  2:3300238/150‑1 (MQ=255)

                                                                          tCGCTAATATCCGGTGCTATAGTGACGTAACAAATCATGCGTGAAAGGGAGAACAAACACGATGAATATTCAGTGCAAACGCGTTTATGATCCGGCTGAACAGAGCGATGGTTATCGCATACTGGTCGACCGCCTCTGGCCGCGCGGTAt                                                                             <  2:9296843/150‑1 (MQ=255)

                                                                           cGCTAATATCCGGTGCTATAGTGACGTAACAAATCATGCGTGAAAGGGAGAACAAACACGATGAATATTCAGTGCAAACGCGTTGATGATCCGGCTGAACAGAGCGATGGTTATCGCATACTGGTCGACCGCCTCTGGCCGCGCGGTATc                                                                            <  2:2430495/150‑1 (MQ=255)

                                                                            gCTAATATCCGGTGCTATAGTGACGTAACAAATCATGCGTGAAAGGGAGAACAAACACGATGAATATTCAGTGCAAACGCGTTTATGATCCGGCTGAACAGAGCGATGGTTATCGCATACTGGTCGACCGCCTCTGGCCGCGCGGTATCa                                                                           <  1:12194836/150‑1 (MQ=255)

                                                                             cTAATATCCGGTGCTATAGTGACGTAACAAATCATGCGTGAAAGGGAGAACAAACACGATGAATATTCAGTGCAAACGCGTTTATGATCCGGCTGAACAGAGCGATGGTTATCGCATACTGGTCGACCGCCTCTGGCCGCGCGGTATCaa                                                                          <  1:5674501/150‑1 (MQ=255)

                                                                              tAATATCCGGTGCTATAGTGACGTAACAAATCATGCGTGAAAGGGAGAACAAACACGATGAATATTCAGTGTAAACGCGTTTATGATCCGGCTGAACAGAGCGATGGTTATCGCATACTGGTCGACCGCCTCTGGCCGCGCGGTATCaaa                                                                         <  1:4253395/150‑1 (MQ=255)

                                                                               aaTATCCGGTGCTATAGTGACGTAACAAATCATGCGTGAAAGGGAGAACAAACACGATGAATATTCAGTGCAAACGCGTTTATGATCCGGCTGAACAGAGCGATGGTTATCGCATACTGGTCGACCGCCTCTGGCCGCGCGGTATCaaaa                                                                        >  2:772013/1‑150 (MQ=255)

                                                                                atatCCGGTGCTATAGTGACGTAACAAATCATGCGTGAAAGGGAGAACAAACACGATGAATATTCAGTGCAAACGCGTTTATGATCCGGCTGAACAGAGCGATGGTTATCGCATACTGGTCGACCGCCTCTGGCCGCGCGGTATCaaaaa                                                                       <  2:4449566/150‑1 (MQ=255)

                                                                                 tatCCGGTGCTATAGTGACGTAACAAATCATGCGTGAAAGGGAGAACAAACACGATGAATATTCAGTGCAAACGCGTTTATGATCCGGCTGAACAGAGCGATGGTTATCGCATACTGGTCGACCGCCTCTGGCCGCGCGGTATCaaaaaa                                                                      <  1:1802316/150‑1 (MQ=255)

                                                                                        tGCTATAGTGACGTAACAAATCATGCGTGAAAGGGAGAACAAACACGATGAATATTCAGTGCAAACGCGTTTATGATCCGGCTGAACAGAGCGATGGTTATCGCATACTGGTCGACCGCCTCTGGCCGCGCGGTATCAAAAAAACCGAtt                                                               <  1:472646/150‑1 (MQ=255)

                                                                                          cTATAGTGACGTAACAAATCATGCGTGAAAGGGAGAACAAACACGATGAATATTCAGTGCAAACGCGTTTATGATCCGGCTGAACAGAGCGATGGTTATCGCATACTGGTCGACCGCCTCTGGCCGCGCGGTATCAAAAAAACCGATTTa                                                             <  2:8225257/150‑1 (MQ=255)

                                                                                           tataGTGACGTAACAAATCATGCGTGAAAGGGAGAACAAACACGATGAATATTCAGTGCAAACGCGTTTATGATCCGGCTGAACAGAGCGATGGTTATCGCATACTGGTCGACCGCCTCTGGCCGCGCGGTATCAAAAAAACCGATTTAg                                                            >  1:5178824/1‑150 (MQ=255)

                                                                                               gTGACGTAACAAATCATGCGTGAAAGGGAGAACAAACACGATGAATATTCAGTGCAAACGCGTTTATGATCCGGCTGAACAGAGCGATGGTTATCGCATACTGGTCGACCGCCTCTGGCCGCGCGGTATCAAAAAAACCGATTTAGCCCt                                                        <  1:8623607/150‑1 (MQ=255)

                                                                                               gTGACGTAACAAATCATGCGTGAAAGGGAGAACAAACACGATGAATATTCAGTGCAAACGCGTTTATGATCCGGCTGAACAGAGCGATGGTTATCGCATACTGGTCGACCGCCTCTGGCCGCGCGGTATCAAAAAAACCGATTTAGCCCt                                                        <  2:7459660/150‑1 (MQ=255)

                                                                                                tGACGTAACAAATCATGCGTGAAAGGGAGACCAAACACGATGAATATTCAGTGCAAACGCGTTTATGATCCGGCTGAACAGAGCGATGGTTATCGCATACTGGTCGACCGCCTCTGGCCGCGCGGTATCAAAAAAACCGATTTAGCCCtt                                                       >  2:2544877/1‑150 (MQ=255)

                                                                                                tGACGTAACAAATCATGCGTGAAAGGGAGAACAAACACGATGAATATTCAGTGCAAACGCGTTTATGATCCGGCTGAACAGAGCGATGGTTATCGCATACTGGTCGACCGCCTCTGGCCGCGCGGTATCAAAAAAACCGATTTAGCCCtt                                                       >  2:9830069/1‑150 (MQ=255)

                                                                                                  aCGTAACAAATCATGCGTGAAAGGGAGAACAAACACGATGAATATTCAGTGCAAACGCGTTTATGATCCGGCTGAACAGAGCGATGGTTATCGCATACTGGTCGACCGCCTCTGGCCGCGCGGTATCAAAAAAACCGATTTAGCCCTtga                                                     <  2:2150852/150‑1 (MQ=255)

                                                                                                   cGTAACAAATCATGCGTGAAAGGGAGAACAAACACGATGAATATTCAGTGCAAACGCGTTTATGATCCGGCTGAACAGAGCGATGGTTATCGCATACTGGTCGACCGCCTCTGGCCGCGCGGTATCAAAAAAACCGATTTAGCCCTtgat                                                    <  2:9701076/150‑1 (MQ=255)

                                                                                                     tAACAAATCATGCGTGAAAGGGAGAACAAACACGATGAATATTCAGTGCAAACGCGTTTATGATCCGGCTGAACAGAGCGATGGTTATCGCATACTGGTCGACCGCCTCTGGCCGCGCGGTATCAAAAAAACCGATTTAGCCCTtgatga                                                  >  2:5406077/1‑150 (MQ=255)

                                                                                                      aaCAAATCATGCGTGAAAGGGAGAACAAACACGATGAATATTCAGTGCAAACGCGTTTATGATCCGGCTGAACAGAGCGATGGTTATCGCATACTGGTCGACCGCCTCTGGCCGCGCGGTATCAAAAAAACCGATTTAGCCCTTGATGAg                                                 <  2:50510/150‑1 (MQ=255)

                                                                                                       aCAAATCATGCGTGAAAGGGAGAACAAACACGATGAATATTCAGTGCAAACGCGTTTATGATCCGGCTGAACAGAGCGATGGTTATCGCATACTGGTCGACCGCCTCTGGCCGCGCGGTATCAAAAAAACCGATTTAGCCCTTGATGAGt                                                >  2:1721618/1‑150 (MQ=255)

                                                                                                       aCAAATCATGCGTGAAAGGGAGAACAAACACGATGAATATTCAGTGCAAACGCGTTTATGATCCGGCTGAACAGAGCGATGGTTATCGCATACTGGTCGACCGCCTCTGGCCGCGCGGTATCAAAAAAACCGATTTAGCCCTTGATGAGt                                                <  1:1320379/150‑1 (MQ=255)

                                                                                                       aCAAATCATGCGTGAAAGGGAGAACAAACACGATGAATATTCAGTGCAAACGCGTTTATGATCCGGCTGAACAGAGCGATGGTTATCGCATACTGGTCGACCGCCTCTGGCCGCGCGGTATCAAAAAAACCGATTTAGCCCTTGATGAGt                                                <  2:1382958/150‑1 (MQ=255)

                                                                                                         aaaTCATGCGTGAAAGGGAGAACAAACACGATGAATATTCAGTGTAAACGCGTTTATGATCCGGCTGAACAGAGCGATGGTTATCGCATACTGGTCGACCGCCTCTGGCCGCGCGGTATCAAAAAAACCGATTTAGCCCTTGATGAGTgg                                              >  1:11692144/1‑150 (MQ=255)

                                                                                                         aaaTCATGCGTGAAAGGGAGAACAAACACGATGAATATTCAGTGCAAACGCGTTTATGATCCGGCTGAACAGAGCGATGGTTATCGCATACTGGTCGACCGCCTCTGGCCGCGCGGTATCAAAAAAACCGATTTAGCCCTTGATGAGTgg                                              >  2:12267376/1‑150 (MQ=255)

                                                                                                         aaaTCATGCGTGAAAGGGAGAACAAACACGATGAATATTCAGTGCAAACGCGTTTATGATCCGGCTGAACAGAGCGATGGTTATCGCATACTGGTCGACCGCCTCTGGCCGCGCGGTATCAAAAAAACCGATTTAGCCCTTGATGAGTgg                                              >  1:7568267/1‑150 (MQ=255)

                                                                                                          aaTCATGCGTGAAAGGGAGAACAAACACGATGAATATTCAGTGCAAACGCGTTTATGATCCGGCTGAACAGAGCGATGGTTATCGCATACTGGTCGACCGCCTCTGGCCGCGCGGTATCAAAAAAACCGATTTAGCCCTTGATGAGTggg                                             <  1:3718664/150‑1 (MQ=255)

                                                                                                            tCATGCGTGAAAGGGAGAACAAACACGATGAATATTCAGTGTAAACGCGTTTATGATCCGGCTGAACAGAGCGATGGTTATCGCATACTGGTCGACCGCCTCTGGCCGCGCGGTATCAAAAAAACCGATTTAGCCCTTGATGAGTGGGAt                                           <  2:11332095/150‑1 (MQ=255)

                                                                                                              aTGCGTGAAAGGGAGAACAAACACGATGAATATTCAGTGCAAACGCGTTTATGATCCGGCTGAACAGAGCGATGGTTATCGCATACTGGTCGACCGCCTCTGGCCGCGCGGTATCAAAAAAACCGATTTAGCCCTTGATGAGTGGGATaa                                         >  2:2934132/1‑150 (MQ=255)

                                                                                                              aTGCGTGAAAGGGAGAACAAACACGATGAATATTCAGTGCAAACGCGTTTATGATCCGGCTGAACAGAGCGATGGTTATCGCATACTGGTCGACCGCCTCTGGCCGCGCGGTATCAAAAAAACCGATTTAGCCCTTGATGAGTGGGATaa                                         >  1:2242304/1‑150 (MQ=255)

                                                                                                               tGCGTGAAAGGGAGAACAAACACGATGAATATTCAGTGCAAACGCGTTTATGATCCGGCTGAACAGAGCGATGGTTATCGCATACTGGTCGACCGCCTCTGGCCGCGCGGTATCAAAAAAACCGATTTAGCCCTTGATGAGTGGGATaaa                                        <  1:4849342/150‑1 (MQ=255)

                                                                                                                      aaGGGAGAACAAACACGATGAATATTCAGTGCAAACGCGTTTATGATCCGGCTGAACAGAGCGATGGTTATCGCATACTGGTCGACCGCCTCTGGCCGCGCGGTATCAAAAAAACCGATTTAGCCCTTGATGAGTGGGATAAAGAAATCa                                 <  1:6920332/150‑1 (MQ=255)

                                                                                                                       aGGGAGAACAAACACGATGAATATTCAGTGCAAACGCGTTTATGATCCGGCTGAACAGAGCGATGGTTATCGCATACTGGTCGACCGCCTCTGGCCGCGCGGTATCAAAAAAACCGATTTAGCCCTTGATGAGTGGGATAAAGAAATCAc                                <  1:10507572/150‑1 (MQ=255)

                                                                                                                         ggAGAACAAACACGATGAATATTCAGTGCAAACGCGTTTATGATCCGGCTGAACAGAGCGATGGTTATCGCATACTGGTCGACCGCCTCTGGCCGCGCGGTATCAAAAAAACCGATTTAGCCCTTGATGAGTGGGATAAAGAAATCACGc                              <  1:10927927/150‑1 (MQ=255)

                                                                                                                          gagaACAAACACGATGAATATTCAGTGCAAACGCGTTTATGATCCGGCTGAACAGAGCGATGGTTATCGCATACTGGTCGACCGCCTCTGGCCGCGCGGTATCAAAAAAACCGATTTAGCCCTTGATGAGTGGGATAAAGAAATCACGcc                             >  1:11832901/1‑150 (MQ=255)

                                                                                                                              acaaacaCGATGAATATTCAGTGCAAACGCGTTTATGATCCGGCTGAACAGAGCGATGGTTATCGCATACTGGTCGACCGCCTCTGGCCGCGCGGTATCAAAAAAACCGATTTAGCCCTTGATGAGTGGGATAAAGAAATCACGCCGTCa                         >  1:1205394/1‑150 (MQ=255)

                                                                                                                              acaaacaCGATGAATATTCAGTGCAAACGCGTTTATGATCCGGCTGAACAGAGCGATGGTTATCGCATACTGGTCGACCGCCTCTGGCCGCGCGGTATCAAAAAAACCGATTTAGCCCTTGATGAGTGGGATAAAGAAATCACGCCGTCa                         <  2:802308/150‑1 (MQ=255)

                                                                                                                              acaaacaCGATGAATATTCAGTGCAAACGCGTTTATGATCCGGCTGAACAGAGCGATGGTTATCGCATACAGGTCGACCGCCTCTGGCCGCGCGGTATCAAAAAAACCGATTTAGCCCTTGATGAGTGGGATAAAGAAATCACGCCGTCa                         >  2:6771770/1‑150 (MQ=255)

                                                                                                                                aaacaCGATGAATATTCAGTGCAAACGCGTTTATGATCCGGCTGAACAGAGCGATGGTTATCGCATACTGGTCGACCGCCTCTGGCCGCGCGGTATCAAAAAAACCGATTTAGCCCTTGATGAGTGGGATAAAGAAATCACGCCGTCAAc                       <  2:6136356/150‑1 (MQ=255)

                                                                                                                                aaacaCGATGAATATTCAGTGCAAACGCGTTTATGATCCGGCTGAACAGAGCGATGGTTATCGCATACTGGTCGACCGCCTCTGGCCGCGCGGTATCAAAAAAACCGATTTAGCCCTTGATGAGTGGGATAAAGAAATCACGCCGTCAAc                       >  1:8185383/1‑150 (MQ=255)

                                                                                                                                  acacGATGAATATTCAGTGCAAACGCGTTTATGATCCGGCTGAACAGAGCGATGGTTATCGCATACTGGTCGACCGCCTCTGGCCGCGCGGTATCAAAAAAACCGATTTAGCCCTTGATGAGTGGGATAAAGAAATCACGCCGTCAACgg                     >  2:12115919/1‑150 (MQ=255)

                                                                                                                                      gATGAATATTCAGTGCAAACGCGTTTATGATCCGGCTGAACAGAGCGATGGTTATCGCATACTGGTCGACCGCCTCTGGCCGCGCGGTATCAAAAAAACCGATTTAGCCCTTGATGAGTGGGATAAAGAAATCACGCCGTCAACGGAACt                 <  1:2815941/150‑1 (MQ=255)

                                                                                                                                        tGAATATTCAGTGCAAACGCGTTTATGATCCGGCTGAACAGAGCGATGGTTATCGCATACTGGTCGACCGCCTCTGGCCGCGCGGTATCAAAAAAACCGATTTAGCCCTTGATGAGTGGGATAAAGAAATCACGCCGTCAACGGAACTgc               <  1:2963066/150‑1 (MQ=255)

                                                                                                                                        tGAATATTCAGTGCAAACGCGTTTATGATCCGGCTGAACAGAGCGATGGTTATCGCATACTGGTCGACCGCCTCTGGCCGCGCGGTATCAAAAAAACCGATTTAGCCCTTGATGAGTGGGATAAAGAAATCACGCCGTCAACGGAACTgc               >  1:7189809/1‑150 (MQ=255)

                                                                                                                                               tCAGTGCAAACGCGTTTATGATCCGGCTGAACAGAGCGATGGTTATCGCATACTGGTCGACCGCCTCTGGCCGCGCGGTATCAAAAAAACCGATTTAGCCCTTGATGAGTGGGATAAAGAAATCACGCCGTCAACGGAACTGCGCAAAGc        >  1:2123710/1‑150 (MQ=255)

                                                                                                                                               tCAGTGCAAACGCGTTTATGATCCGGCTGAACAGAGCGATGGTTATCGCATACTGGTCGACCGCCTCTGGCCGCGCGGTATCAAAAAAACCGATTTAGCCCTTGATGAGTGGGATAAAGAAATCACGCCGTCAACGGAACTGCGCAAAGc        <  1:1003910/150‑1 (MQ=255)

                                                                                                                                                cAGTGCAAACGCGTTTATGATCCGGCTGAACAGAGCGATGGTTATCGCATACTGGTCGACCGCCTCTGGCCGCGCGGTATCAAAAAAACCGATTTAGCCCTTGATGAGTGGGATAAAGAAATCACGCCGTCAACGGAACTGCGCAAAGcc       >  2:6389231/1‑150 (MQ=255)

                                                                                                                                                 aGTGCAAACGCGTTTATGATCCGGCTGAACAGAGCGATGGTTATCGCATACTGGTCGACCGCCTCTGGCCGCGCGGTATCAAAAAAACCGATTTAGCCCTTGATGAGTGGGATAAAGAAATCACGCCGTCAACGGAACTGCGCAAAGCCt      >  1:4377025/1‑150 (MQ=255)

                                                                                                                                                  gTGCAAACGCGTTTATGATCCGGCTGAACAGAGCGATGGTTATCGCATACTGGTCGACCGCCTCTGGCCGCGCGGTATCAAAAAAACCGATTTAGCCCTTGATGAGTGGGATAAAGAAATCACGCCGTCAACGGAACTGCGCAAAGCCtt     >  1:7390160/1‑150 (MQ=255)

                                                                                                                                                   tGCAAACGCGTTTATGATCCGGCTGAACAGAGCGATGGTTATCGCATACTGGTCGACCGCCTCTGGCCGCGCGGTATCAAAAAAACCGATTTAGCCCTTGATGAGTGGGATAAAGAAATCACGCCGTCAACGGAACTGCGCAAAGCCttt    <  1:5321863/150‑1 (MQ=255)

                                                                                                                                                     cAAACGCGTTTATGATCCGGCTGAACAGAGCGATGGTTATCGCATACTGGTCGACCGCCTCTGGCCGCGCGGTATCAAAAAAACCGATTTAGCCCTTGATGAGTGGGATAAAGAAATCACGCCGTCAACGGAACTGCGCAAAGCCTTTCa  <  2:1955008/150‑1 (MQ=255)

                                                                                                                                                     cAAACGCGTTTATGATCCGGCTGAACAGAGCGATGGTTATCGCATACTGGTCGACCGCCTCTGGCCGCGCGGTATCAAAAAAACCGATTTAGCCCTTGATGAGTGGGATAAAGAAATCACGCCGTCAACGGAACTGCGCAAAGCCTTTCa  >  2:4366451/1‑150 (MQ=255)

CCACTTATGGGTGTTGCCATACTTTCACTACTGATGGCGATTTTCGGACTTTGCGCCGGGAGAGACAAAGAAATTCGCTAATATCCGGTGCTATAGTGACGTAACAAATCATGCGTGAAAGGGAGAACAAACACGATGAATATTCAGTGCAAACGCGTTTATGATCCGGCTGAACAGAGCGATGGTTATCGCATACTGGTCGACCGCCTCTGGCCGCGCGGTATCAAAAAAACCGATTTAGCCCTTGATGAGTGGGATAAAGAAATCACGCCGTCAACGGAACTGCGCAAAGCCTTTCA  >  NC_000913/1876774‑1877072

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`