Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,177,234 C→A 14.4% intergenic (‑30/+278) gatY ← / ← fbaB D‑tagatose 1,6‑bisphosphate aldolase 2, catalytic subunit/fructose‑bisphosphate aldolase class I

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,177,2340CA14.4% 1322.9 / 47.1 558intergenic (‑30/+278)gatY/fbaBD‑tagatose 1,6‑bisphosphate aldolase 2, catalytic subunit/fructose‑bisphosphate aldolase class I
Reads supporting (aligned to +/- strand):  ref base C (196/253);  new base A (63/42);  total (263/295)
Fisher's exact test for biased strand distribution p-value = 3.24e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 573 total aligned reads displayed.

GTTGGCAGCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGG  >  NC_000913/2177085‑2177383
                                                                                                                                                     |                                                                                                                                                     
gTTGGCAGCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTc                                                                                                                                                       >  2:2044402/1‑150 (MQ=255)
  tGGCAGCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCaa                                                                                                                                                     <  2:4375652/150‑1 (MQ=255)
  tGGCAGCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCaa                                                                                                                                                     <  1:4386954/150‑1 (MQ=255)
    gCAGCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGATTTCaaaa                                                                                                                                                   >  1:10463436/1‑150 (MQ=255)
     cAGCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTTTTTTAGATTTAAAAat                                                                                                                                                  >  1:873368/1‑150 (MQ=255)
     cAGCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAat                                                                                                                                                  <  1:6098264/150‑1 (MQ=255)
      aGCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGGTTTCGGTTTCAAAata                                                                                                                                                 >  1:101316/1‑150 (MQ=255)
           tttCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATg                                                                                                                                            >  2:7702790/1‑150 (MQ=255)
            ttCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTCGATTTCAAAATATAATGa                                                                                                                                           >  2:5674983/1‑150 (MQ=255)
            ttCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTCGATTTCAAAATATAATGa                                                                                                                                           >  1:2585918/1‑150 (MQ=255)
                accaccACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAatt                                                                                                                                       <  1:9408677/150‑1 (MQ=255)
                 ccaccaCTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAatta                                                                                                                                      >  2:5125631/1‑150 (MQ=255)
                  caccacTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAattat                                                                                                                                     <  2:9296435/150‑1 (MQ=255)
                  caccacTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGATTTCAAAATATAATGAAaatat                                                                                                                                     >  1:9577271/1‑150 (MQ=255)
                    ccacTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTTTTTTAGATTTAAAAATATAATGAAAATAttt                                                                                                                                   >  2:5381067/1‑150 (MQ=255)
                    ccacTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTAttt                                                                                                                                   <  2:3108618/150‑1 (MQ=255)
                        gtGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGttt                                                                                                                               <  1:2226980/149‑1 (MQ=255)
                         tGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGtttt                                                                                                                              <  2:2211685/150‑1 (MQ=255)
                           cATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTa                                                                                                                            <  1:4862014/150‑1 (MQ=255)
                           cATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTACTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTa                                                                                                                            <  2:4144827/150‑1 (MQ=255)
                                tttCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTAAAAATATAATTAAAATATTTTTTTTTAAatat                                                                                                                       >  1:12147594/1‑150 (MQ=255)
                                    gagaTTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTTTTTTAGATTTCAAAATATAATTAAATTAATTTTTTTTAAATATAgat                                                                                                                   >  2:90832/1‑149 (MQ=255)
                                       aTTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTTTTTTAGATTTAAAAAAATAAATAAAATATTTGTTTTTAAATATAGAGATa                                                                                                                >  2:5283920/1‑150 (MQ=255)
                                           tgAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGa                                                                                                            <  1:6962386/150‑1 (MQ=255)
                                           tgAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTTTTTTAGAATTAAAAATATAATTAAATTATTTTTTTTTAAATATAGAGTTAAAGa                                                                                                            >  1:11534000/1‑150 (MQ=255)
                                            gAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTTTTTTAGATTTAAAAATATAATGAAAATATTTGTTTTTAAATATAGAGATaaacat                                                                                                           >  2:3251764/1‑146 (MQ=255)
                                            gAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTTTTTTTAAATATAGTGATAACGAt                                                                                                           >  2:4877490/1‑150 (MQ=255)
                                            gAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGAt                                                                                                           <  1:4710715/150‑1 (MQ=255)
                                            gAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGAt                                                                                                           <  1:8544712/150‑1 (MQ=255)
                                            gAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGAt                                                                                                           <  1:5663618/150‑1 (MQ=255)
                                             aaTATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGAATTCAAAATATAATTAAATTATTTTTTTTTAAATATCGAGATAACGATc                                                                                                          >  1:6502558/1‑150 (MQ=255)
                                               tatTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTCGATTTCAAAATATAATGAAAATATTTTTTTTTAAATATAGAGATAAAGATgac                                                                                                        >  1:763568/1‑150 (MQ=255)
                                                 ttGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACaa                                                                                                      <  1:6414185/150‑1 (MQ=255)
                                                    aaTGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCTTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACaaaaa                                                                                                   <  2:9545451/150‑1 (MQ=255)
                                                    aaTGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACaaaaa                                                                                                   <  1:304145/150‑1 (MQ=255)
                                                    aaTGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACaaaaa                                                                                                   <  1:2477875/150‑1 (MQ=255)
                                                    aaTGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATAGAGATAACGATAACaaaaa                                                                                                   >  2:10432230/1‑150 (MQ=255)
                                                    aaTGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTAGATTTGAAAATATAATGAAATTATTTTTTTTTAAATATAGAGATAACGATCACaaaaa                                                                                                   >  1:3879257/1‑150 (MQ=255)
                                                     aTGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAAc                                                                                                  <  1:3474158/150‑1 (MQ=255)
                                                       gCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGa                                                                                                <  2:2481212/150‑1 (MQ=255)
                                                       gCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGa                                                                                                <  2:3884750/150‑1 (MQ=255)
                                                       gCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGa                                                                                                <  2:4548039/150‑1 (MQ=255)
                                                       gCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTAGATTTAAAAATATAAATAAATTATTTTTTTTTAAAAATAGAGATAAAGATCAAAAAAACGa                                                                                                >  1:13687/1‑150 (MQ=255)
                                                        ccGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTTTTTTCGATTTCAAAATATAATTAAATTATTTGTTTTTAAATAAAGAGATAACGAACAAAAAAAAGAc                                                                                               >  2:468603/1‑150 (MQ=255)
                                                        ccGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTTTTTTCGATTTCAAAATATAATGAAATTATTTTTTTTTAAATATCGAGTTAACGATCAAAAAAACGAc                                                                                               >  1:6621730/1‑150 (MQ=255)
                                                         cGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTTTTTTCGATTTAAAAAAATAATTTAATTATTTTTTTTTAAATATCGAGATAACGATAACAAAAAAGACa                                                                                              >  1:9238664/1‑150 (MQ=255)
                                                         cGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTTTTTTTAAATATGTAGATGACGATCACAAAAAAGaaa                                                                                              >  1:7519845/1‑148 (MQ=255)
                                                          ggAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATTAAATTATTTGTTTTTAAATTTAGAGCTAACGATCACAAAAAAGACaa                                                                                             >  1:2843057/1‑150 (MQ=255)
                                                                 cATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCGTTGTCGATACCACGTACAGTTTCATATCCTGTCGTGTGTTTTCGATTTCAAAATAAAAAGAAAATATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACCAAATgcca                                                                                      >  2:3483514/1‑147 (MQ=255)
                                                                  aTAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGATTTCAAAATATAATGAAATTATTTTTTTTTAAATATAGAGATAAAGATAACAAAAAAGACAAAATGaaaa                                                                                     >  1:1954284/1‑150 (MQ=255)
                                                                       cgccgcGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAattatt                                                                                <  2:6175217/150‑1 (MQ=255)
                                                                        gccgcGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGTAAATATTTGTTTTTAAATATAGAGATAAAGATCACAAAAACGACAAGATGGAAATTATTc                                                                               >  1:816797/1‑150 (MQ=255)
                                                                          cgcgcgGGGCGTTGTTCAGCATCTGCTTCGTCGATACCACGAACATTTACATATCCGGTCGTTTGTTTTCGATTACAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCga                                                                             <  2:7385737/150‑1 (MQ=255)
                                                                          cgcgCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCga                                                                             <  2:6828266/150‑1 (MQ=255)
                                                                           gcgcTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCgag                                                                            <  2:8164746/150‑1 (MQ=255)
                                                                            cgcTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATAGAGATAAAGCTCACAAAAACGACAATATGAAAattattagtgg                                                                           >  2:11782800/1‑145 (MQ=255)
                                                                             gcTGTGCGTTGTTCAGCATCTGATTTGTCTATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATATTGAAATTATTTGTTTTTAAATATCGATATACCTATCACAAAAACGACAATATGAAAATTATTCgagga                                                                          <  2:3418609/150‑1 (MQ=255)
                                                                              cTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGATTTAAAAAAAAAAATAAAATATTTTTTTTTAAATATAGAGTTAACGATAAAAAAAAAGACAAGATGAAAAATATTAtaggag                                                                         >  1:549851/1‑150 (MQ=255)
                                                                                   cGTTGTTCAGAATCTGCTTTTTCTATACCACGTACATTTTCTTATCCTGTCGTTTGTTTTCGATTTCAAATTATAATGAAATTATTTGTTTTTATATATCGAGATAACGTTCACAAAAACGACAATATGAAAATTATTCGAGGAGTGaaa                                                                    <  2:4993342/150‑1 (MQ=255)
                                                                                         taagaaTCTGTTTTGTCGATACCAATTAAATTTTCATATCGTGTGGTTTGTTTTCTATTTCAAAATTTAATTAATTTTTTTTTTTTTAAATATCTAGATACCGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCaaa                                                              <  1:5409429/145‑1 (MQ=255)
                                                                                             cATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTTTTTTGGAATTAAAAAAAAAAATAAAAAAATTTTTTTTTAATAAAGCGATAACCAAACCAAAAAAAACACAAATAAAAATAATCGAGGAGTGGTAGGCaaaaaaa                                                          >  1:10284668/1‑150 (MQ=255)
                                                                                              aTCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAAc                                                         <  2:5823085/150‑1 (MQ=255)
                                                                                              aTCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAAc                                                         <  2:7547650/150‑1 (MQ=255)
                                                                                                 tGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGATATAACGATAACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGc                                                      <  2:1920651/150‑1 (MQ=255)
                                                                                                 tGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGc                                                      >  2:10140452/1‑150 (MQ=255)
                                                                                                 tGCTTTGTCGATACAACTTAATTTTTCTTATCCTTTCTTTTGTTTTCGTTTTAAAAATATATTTAATTTATTTTTTTTTAAATATCTATATAACTTTCAAAAAAACTACAATATGAAATTTATTCGAGGAGTGAAAGGCAAAAAAACGGc                                                      <  2:10513220/150‑1 (MQ=255)
                                                                                                    tttGTATATACCACGTAAATTTTAATATCCTGTCGTTTTTTTTCTATTTAAAAATATAATGAAATTATTTTTTTTTAAATATCGATATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTc                                                   <  2:8041473/150‑1 (MQ=255)
                                                                                                        tCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGa                                               <  2:604753/150‑1 (MQ=255)
                                                                                                          gATACAACTTAATTTTTCATTTCGTTTGTTTTTTTTTCTTTTTAAAAATATATTTAATTTTTTTTTTTTTAATTATCTTTATACCGAACAAAAAAACGACAATATTAAAATTTTTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATa                                             <  1:1174448/150‑1 (MQ=255)
                                                                                                           aTACCCCTTACATTTTCATATCCTGTTTTTTTTTTTCTTTTTAAAAATATAATTAATTTTTTTTTTTTTAATTATCTTTATAACGAACAAAAAAACGACAATTTGAAAATTATTCGAGGTGTGAAAGGCAAAAAAACGGCCTCCCGATAg                                            <  1:5166784/150‑1 (MQ=255)
                                                                                                           aTACCACTTAATTTTTCATATCCTTTGTTTTTTTTTGTTTTTAAAAATTTAATTAATTTTTTTTTTTTTAAATATCTATATACCGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGAAAAAAAACGGCCTCCCGATAg                                            <  1:419109/150‑1 (MQ=255)
                                                                                                             aCCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAggg                                          <  2:2971172/150‑1 (MQ=255)
                                                                                                               cACGTAATTTTTCATATCGGGTGGTTTGTTTTCTATTTCAAAATAAAATAAATTTTTTTTTTTTAAAATATCTACATAACGATCACAAAAACGACAATATTAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGaa                                        <  1:1661414/150‑1 (MQ=255)
                                                                                                                  gTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGcc                                     <  2:8665109/150‑1 (MQ=255)
                                                                                                                    aCATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTTTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAAAGGCCTCCCGATAGGGAAGCCGt                                   >  2:5972522/1‑150 (MQ=255)
                                                                                                                     atttttCATACCCTGTGTTTTTTTTTCTATTTCAAAATATATTTAAATTTTTTTTTTTTAAATATCGCTAAACCTATCAAAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTa                                  <  1:6195984/148‑1 (MQ=255)
                                                                                                                      aTTTTCATATCCTGTCTTTTTTTTTCTATTTAAAAATATAATTAAATTATTTTTTTTTAAATATCGAGATAACTATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAg                                 <  1:9896282/150‑1 (MQ=255)
                                                                                                                               tCCTGTCGTTTTTTTTCTATTTCAAAATATAATGAAATTTTTTTTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTgcg                        <  1:3691932/150‑1 (MQ=255)
                                                                                                                                ccTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTgcgc                       <  2:12263785/150‑1 (MQ=255)
                                                                                                                                 cTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCCAGATAACGATCACAAAAACCACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCCAAAGGGAAGCCCTAGCAAAGGGcgcg                      >  2:6568591/1‑150 (MQ=255)
                                                                                                                                  tGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCgt                     <  2:8433422/150‑1 (MQ=255)
                                                                                                                                  tGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCgt                     <  2:11244110/150‑1 (MQ=255)
                                                                                                                                     cGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGATATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGtt                  <  2:11620716/150‑1 (MQ=255)
                                                                                                                                      gtttgtttTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGttt                 >  2:10937012/1‑150 (MQ=255)
                                                                                                                                      gtttgtttTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGttt                 >  1:10918322/1‑150 (MQ=255)
                                                                                                                                        ttgtttTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATCGGGACGCCGTAGCAAAGTGCGCGTGttttt               >  2:8348222/1‑150 (MQ=255)
                                                                                                                                        ttgtttTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGttttt               >  1:10696369/1‑150 (MQ=255)
                                                                                                                                           ttttCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATg            >  1:7803786/1‑150 (MQ=255)
                                                                                                                                           ttttCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATg            >  2:7326196/1‑150 (MQ=255)
                                                                                                                                            tttCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATgt           >  2:159378/1‑149 (MQ=255)
                                                                                                                                            tttCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGc           >  2:9464807/1‑150 (MQ=255)
                                                                                                                                             ttCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGcc          >  2:10758180/1‑150 (MQ=255)
                                                                                                                                             ttCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGcc          >  2:8517010/1‑150 (MQ=255)
                                                                                                                                             ttCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGcc          >  2:276223/1‑150 (MQ=255)
                                                                                                                                              tctaTTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAACACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCg         <  2:9803552/147‑1 (MQ=255)
                                                                                                                                                   ttCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGc    <  1:10033200/150‑1 (MQ=255)
                                                                                                                                                     cAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGTGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCgg  <  2:10678403/150‑1 (MQ=255)
                                                                                                                                                     cAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCgg  >  1:8847768/1‑150 (MQ=255)
                                                                                                                                                     cAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCgg  >  1:725814/1‑150 (MQ=255)
                                                                                                                                                     cAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAAACGCCACCCCTTAAGTAAGCCGTATGACAGTTCGCCGGGTTTTAATCCGGATgacg  >  1:11094619/1‑147 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
GTTGGCAGCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGG  >  NC_000913/2177085‑2177383

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: