Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,454,266 C→A 5.1% pseudogene (736/2646 nt) yfcU ← pseudogene, FimD fimbrial export usher family;putative membrane; Not classified; putative outer membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,454,2660CA 5.1% 3312.0 / 16.9 1028pseudogene (736/2646 nt)yfcUpseudogene, FimD fimbrial export usher family;putative membrane; Not classified; putative outer membrane protein
Reads supporting (aligned to +/- strand):  ref base C (506/429);  new base A (3/90);  total (509/519)
Fisher's exact test for biased strand distribution p-value = 7.19e-25
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 1051 total aligned reads displayed.

TGGCGCGTAGCCGCGCAGATTGGGAGGCAACATTTGATCGTCAGTGCTGACACTGCCACCAACATAATTAAAACCGTCGAAAATATCGGAATTGAGGTAATCTTCGCCCAGCGCCAGTTTGGCTTTTAATGACGGTAACGCCCGCCAGGCATAATAGCGACTCCACTCCCATTTTTTTTGTGTGTCATCGCCGCCAAATTCATCGTCATCATCATTACTGCGCGTATGTTGATAGTTGGTCTGCCAGTCAGCGCGCATACGCCATGGCCCCAGGTTAACCCCGACCGTCCCGTTGCCGC  >  NC_000913/2454117‑2454415
                                                                                                                                                     |                                                                                                                                                      
tGGCGCGTAGCCGCGCAGATTGGGAGGCAACATTTGATCGTCAGTGCTGACACTGCCACCAACATAATTAAAACCGTCGAAAATATCGGAATTGAGGTAATCTTCGCCCAGCGCCAGTTTGGCTTTTAATGACGGTAACGCCCGCCAGGc                                                                                                                                                        >  2:4007672/1‑150 (MQ=255)
  gcgcGTCGCCGCGCAGATTGGGAGGCAACATTTGATCGTCAGTGCTGACACTGCCACCAACATAATTAAAACCGTCGAAAATATCGGAATTGAGGTAATCTTCGCCCAGCGCCAGTTTGGCTTTTAATGACGGTAACGCCCGCCAGGCat                                                                                                                                                      <  1:9811436/150‑1 (MQ=255)
  gcgcGTAGCCGCGCAGATTGGGAGGCAACATTTGATCGTCAGTGCTGACACTGCCACCAACATAATTAACACCGTCGAAAATATCGGAATTGAGGTAATCTTCGCCCAGCGCCAGTTTGGCTTTTAATGACGGTAACGCCCGCCAGGCat                                                                                                                                                      <  1:7132396/150‑1 (MQ=255)
  gcgcGTAGCCGCGCAGATTGGGAGGCAACATTTGATCGTCAGTGCTGACACTGCCACCAACATAATTAAAACTGTCGAAAATATCGGAATTGAGGTAATCTTCGCCCAGCGCCAGTTTGGCTTTTAATGACGGTAACGCCCGCCAGGCat                                                                                                                                                      <  1:11247758/150‑1 (MQ=255)
  gcgcGTAGCCGCGCAGATTGGGAGGCAACATTTGATCGTCAGTGCTGACACTGCCACCAACATAATTAAAACCGTCGAAAATATCGGAATTGAGGTAATCTTCGCCCAGCGCCAGTTTGGCTTTTAATGACGGTAACGCCCGCCAGGCat                                                                                                                                                      >  1:7603992/1‑150 (MQ=255)
    gcgTAGCCGCGCAGATTGGGAGGCAACATTTGATCGTCAGTGCTGACACTGCCACCAACATAATTACAACCGTCGAAAATATCGGAATTGAGGTAATCTTCGCCCAGCGCCAGTTTGGCTTTTAATGACGGTAACGCCCGCCAGGCataa                                                                                                                                                    <  2:3350665/150‑1 (MQ=255)
    gcgTAGCCGCGCAGATTGGGAGGCAACATTTGATCGTCAGTGCTGACACTGCCACCAACATAATTAAAACCGTCGAAAATATCGGAATTGAGGTAATCTTCGCCCAGCGCCAGTTTGGCTTTTAATGACGGTAACGCCCGCCAGGCataa                                                                                                                                                    >  2:5168601/1‑150 (MQ=255)
     cgTAGCCGCGCAGATTGGGAGGCAACATTTGATCGTCAGTGCTGACACTGCCACCAACATAATTAAAACCGTCGAAAATATCGGAATTGAGGTAATCTTCGCCCAGCGCCAGTTTGGCTTTTAATGACGGTAACGCCCGCCAGGCataat                                                                                                                                                   <  2:2832536/150‑1 (MQ=255)
        aGCCGCGCAGATTGGGAGGCAACATTTGATCGTCAGTGCTGACACTGCCACCAACATAATTAAAACCGTCGAAAATATCGGAATTGAGGTAATCTTCGCCCAGCGCCAGTTTGGCTTTTAATGACGGTAACGCCCGCCAGGCATAATAGc                                                                                                                                                <  2:2458246/150‑1 (MQ=255)
          ccGCGCAGATTGGGAGGCAACATTTGATCGTCAGTGCTGACACTGCCACCAACATAATTAAAACCGTCGAAAATATCGGAATTGAGGTAATCTTCGCCCAGCGCCAGTTTGGCTTTTAATGACGGTAACGCCCGCCAGGCATAATAGCGa                                                                                                                                              <  2:4322836/150‑1 (MQ=255)
          ccGCGCAGATTGGGAGGCAACATTTGATCGTCAGTGCTGACACTGCCACCAACATAATTAAAACCGTCGAAAATATCGGAATTGAGGTAATCTTCGCCCAGCGCCAGTTTGGCTTTTAATGACGGTAACGCCCGCCAGGCATAATAGCGa                                                                                                                                              >  2:1508773/1‑150 (MQ=255)
                   ttGGGAGGCAACATTTGATCGTCAGTGCTGACACTGCCACCAACATAATTAAAACCGTCGAAAATATCGGAATTGAGGTAATCTTCGCCCAGCGCCAGTTTGGCTTTTAATGACGGTAACGCCCGCCAGGCATAATAGCGactccactcc                                                                                                                                     >  1:18886/1‑150 (MQ=255)
                      ggAGGCAACATTTGATCGTCAGTGCTGACACTGCCACCAACATAATTAAAACGGTCGAAAATATCGGAATTGTGGTAATCTTCGCCCAGCGCCAGTTTGGCTTTTATTGACGGTAACGCCCGCCAGGCATAATAGCGACTCCACTCCCAt                                                                                                                                 <  2:6950675/150‑1 (MQ=255)
                          gCAACATTTGATCTTCAGTGCTGACACTGCCACCAACATAATTAAAACCGTCGAAAATATCGGATTTGAGGTAATCTTCGCCCAGCGCCTGTTTGGCTTTTAATGACGGTAACGCCCGCCTGGAATAATAGCGACTCCACTCCCAttttt                                                                                                                             <  2:4443383/150‑1 (MQ=255)
                          gCAACATTTGATCGTCAGTGCTGACACTGCCACCAACATAATTAAAACCGTCGAAAATATCGGAATTGAGGTAATCTTCGCCCAGCGCCAGTTTGGCTTTTAATGACGGTAACGCCCGCCAGGCATAATAGCGACTCCACTCCCAttttt                                                                                                                             >  2:462633/1‑150 (MQ=255)
                            aaCATTTGATCGTCAGTGCTGACACTGCCACCAACATAATTAAAACCGTCTAAAATATCGGAATTGAGGTAATCTTCGCCCAGCGCCAGTTTGGCTTTTAATGACGGTAACGCCCGCCAGGCATAATAGCGACTCCACTCCCAttttttt                                                                                                                           <  2:1349435/150‑1 (MQ=255)
                            aaCATTTGATCGTCAGTGCTGACACTGCCACCAACATAATTAAAACCGTCGAAAATATCGGAATTGAGGTAATCTTCGCCCAGCGCCAGTTTGGCTTTTAATGACGGTAACGCCCGCCAGGCATAATAGCGACTCCACTCCCAttttttt                                                                                                                           >  2:6070621/1‑150 (MQ=255)
                            aaCATTGGATCGTCAGTGCTGACACTGCCACCAACATAATTACACCCGTCGAAAATATCGGATTTGAGGTAATCTTCGCCCCGCGCCATTTTGGCTTTTAATGACGGTAACGCCCGCCAGGCATATTAGCGACTCCACTCCCAttttttt                                                                                                                           <  2:9711063/150‑1 (MQ=255)
                               aTTTGATCGTCAGTGCTGACACTGCCACCAACATAATTAAAACCGTCGAAAATATCGGAATTGAGGTAATCTTCGCCCAGCGCCAGTTTGGCTTTTAATGACGGTAACGCCCGCCAGGCATAATAGCGACTCCACTCCCATTTTTTTtgt                                                                                                                        >  2:10523851/1‑150 (MQ=255)
                                 tttaTCGTCTGTGCTGACACTGCCACCAAAATAATTAAAACCGTCGAAAATATCGGATTTGAGTTAATCTTCGCCCAGCGCCTGTTTGGCTTTTAATGAGGTTAACGCCCGCCAGGAATAATAGCGACTCCACTCCCATTTTTTTtgtgt                                                                                                                      <  2:1110964/147‑1 (MQ=255)
                                  tGATCGTCAGTGCTGACACTGCCACCAACATAATTAAAACCGTCGAAAATATCGGAATTGAGGTAATCTTCGCCCAGCGCCAGTTTGGCTTTTAATGACGGTAACGCCCGCCAGGCATAATAGCGACTCCACTCCCATTTTTTTtgtgtg                                                                                                                     >  1:10032798/1‑150 (MQ=255)
                                       gTCAGTGCTGACACTGCCACCAACATAATTAAGACCGTCGAAAATATCGGATTTGAGGTAATCTTCGCCCAGCGCCTGTTTGGCTTTTAATGACGGTAACGCCCGCCAGGCATAATAGCGACTCCACTCCCATTTTTTTTGTGTGTCATc                                                                                                                <  2:578762/150‑1 (MQ=255)
                                                gACACTGCCACCAACAGAATTAAAACCGTCGAAAATATCGGACTTGAGGTAATCTTCGCCCAGCGCCAGTTTGGCTTTTAATGACGGTAACGCCCGCCAGGCATAATAGCGACTCCACTCCCATTTTTTTTGGGTGTCATCGCCGCCaaa                                                                                                       >  2:3179275/1‑150 (MQ=255)
                                                    ccgCCACCAAAATAATTAAACCCGTCTAAAATATCGTATTTGCGGTAATCTGCGCCCAGCGCCTTTTTGGCTTTTAATGACGTTACCGCCCGCCAGGAATAACAGCGACTCCACTCCCATTTTTTTTGTGTGTCATCGCCGCCAAATTCa                                                                                                   <  1:2931129/148‑1 (MQ=255)
                                                     tGCCACCAACATAATTAAAACCGTCGAAAATATCGGAATTGAGGTAATCTTCGCCCAGCGCCAGTTTGGCTTTTAATGACGGTAACGCCCGCCAGGCATAATAGCGACTCCACTCCCATTTTTTTTGTGTGTCATCGCCGCCAAATTCAt                                                                                                  >  1:6259633/1‑150 (MQ=255)
                                                     tGCCACCAACATAATTAAAACCGTCGAAAATATCGGAATTGAGGTAATCTTCGCCCAGCGCCAGTTTGGCTTTTAATGACGGTAACGCCCGCCAGGCATAATAGCGACTCCACTCCCATTTTTTTTGTGTGTCATCGCCGCCAAATTCAt                                                                                                  >  2:1968598/1‑150 (MQ=255)
                                                     tGCCACCAACATAATTAAAACCGTCGAAAATATCGGAATTGAGGTAATCTTCGCCCAGCGCCAGTTTGGCTTTTAATGACGGTAACGCCCGCCAGGCATAATAGCGACTCCACTCCCATTTTTTTTGTGTGTCATCGCCGCCAAATTCAt                                                                                                  >  2:2256221/1‑150 (MQ=255)
                                                     tGCCACCAACATAATTAAAACCGTCGAAAATATCGGAATTGAGGTAATCTTCGCCCAGCGACAGTTAGGCTTTTAATGACGTTAACGCCCGCCAGGCATAATAGCGACTCCACTCCCATTTTTTTTGTGTGTCATCGCCGCCAAATACAt                                                                                                  >  2:7778181/1‑150 (MQ=255)
                                                     ggCCACAAAATTAATAAAAACGGGCTAAAATTTGGGATTTGTGGTTTTCTGCGCCCGGGGCCTGTTGGGTTTTTATTGAGGTTAACGCCGGCCTGGCATAATCGCGACTCCACTCCCATTTTTTTTGTGTGTCATCGCCGCCAAATTCAt                                                                                                  <  1:2603746/149‑1 (MQ=255)
                                                      cccaccaAAATAATTAAACCCGTCTAAAATATCGGATTTGTGTTAATGTCCGCCCAGGGCCTTTTTGGCTTTTAATGACGGAAACGCCCGCCAGCAATAATCGCGACTCCACTCCCATTTTTTTTGTGTGTCATCGCCGCCAAATTCATc                                                                                                 <  1:11404944/149‑1 (MQ=255)
                                                         accaACATAATTAAAACCGTCGAAAATATCGGAATTGAGGTAATCTTCGCCCAGCGCCAGTTTGGCTTTTAATGACGGTAACGCCCGCCAGGCATAATAGCGACTCCACTCCCATTTTTTTTGTGTGTCATCGCCGCCAAATTCATCGtc                                                                                              >  1:5129023/1‑150 (MQ=255)
                                                         accaACATAATTAAAACCGTCGAAAATATCGGAATTGAGGTAATCTTCGCCCAGCGCCAGTTAGGCTTTTAATGACGGTAACGCCCGCCAGGCATAATAGCGACTCCACTCCCATTTTTTTTGTGTGTCATCGCCGCCAAATTCATCGtc                                                                                              >  1:3848688/1‑150 (MQ=255)
                                                            aaCATAATTAAAACCTTCTAAAATATCGCAATTGTGTTAATCTCCGCCCAGCGCCAGTTTGGATTTTAAGCACGTTACCGCCCGCCAGGCATAATCGCGACTCCACTCCCATTTTTTTTGTGTGTCATCGCCGCCAAATTCATCGtcatc                                                                                           <  1:5747913/150‑1 (MQ=255)
                                                            aaCATAATTAAAACCGTCGAAAATATCGGAATTGAGGTAATCTTCGCCCAGCGCCAGTTTGGCTTTTAATGACGGTAACGCCCGCCAGGCATAATAGCGACTCCACTCCCATTTTTTTTGTGTGTCATCGCCGCCAAATTCATCGtcatc                                                                                           >  2:4766214/1‑150 (MQ=255)
                                                            aaCATAATTAAAACCGTCGAAAATATCGGAATTGAGGTAATCTTCGCCCAGCGCCAGTTTGGCTTTTAATGACGGTAACGCCCGCCAGGCATAATAGCGACTCCACTCCCATTTTTTTTGTGTGTCATCGCCGCCAAATTCATCGtcatc                                                                                           >  2:656173/1‑150 (MQ=255)
                                                             aCATAATTAAAACCGTCGAAAATATCGGAATTGAGGTAATCTTCGCCCAGCGCCAGTTTGGCTTTTAATGACGGTAACGCCCGCCAGGCATAATAGCGACTCCACTCCCATTTTTTTTTGTGTGTCATCGCCGCCAAATTCATCGtcatc                                                                                           >  1:6907800/1‑150 (MQ=255)
                                                              cATAATTAAAACCGTCGAAAATATCGGAATTGAGGTAATCTTCGCCCAGCGCCAGTTTGGCTTTTAATGACGGTAACGCCCGCCAGGCATAATAGCGACTCCACTCCCATTTTTTTTGTGTGTCATCGCCGCCAAATTCATCGtcatcat                                                                                         >  1:3071450/1‑150 (MQ=255)
                                                               ctAATTAAACCGGTCGAAAATCTCGGAATTGTGGTAATCTTCGCCCAGCGCCAGTTTGGCTTTTATGGAGGGTAACGCCCGCCTGGCATAATAGCGACTCCACTCCCATTTTTTTTGTGTGTCATCGCCGCCAAATTCATCGtcatcatc                                                                                        <  2:9533086/149‑1 (MQ=255)
                                                               aTAATTAAAACCGTCGAAAATATCGGATTTGAGGTAATCTTCGCCCAGCGCCAGTTTGGCTTTTAATGACGGTAACGCCCGCCAGGCATAATAGCGACTCCACTCCCATTTTTTTTGTGTGTCATCGCCGCCAAATTCATCGtcatcatc                                                                                        <  2:3729716/150‑1 (MQ=255)
                                                               aTAATTAAAACCGTCGAAAATAACGGAATTGAGGTAATCTTCGCCCAGCGCCATTTTGGCTTTTAATGACGGTAACGCCCGCCAGGCATAATAGCGACTCCACTCCCATTTTTTTTGTGTGTCATCGCCGCCAAATTCATCGtcatcatc                                                                                        <  2:1706912/150‑1 (MQ=255)
                                                                tAATTAAAACCGTCTAAATTTTGTTTTTTGTGGTAATCTTCGCCCAGCGCCTTTTTGGCTTTTAATGAGGTTAACGCCCGCCAGGAATAATAGCGACTCCACTCCCATTTTTTTTGTGTGTCATCGCCGCCAAATTCATCGtcatcatca                                                                                       <  1:331486/150‑1 (MQ=255)
                                                                tAATTAAAACCGTCGAAAATATCGGAATTGAGGTAATCTTCGCCCAGCGCCAGTTTGGCTTTTAATGACGGTAACGCCCGCCAGGCATAATAGCGACTCCACTCCCATTTTTTTTGTGTGTCATCGCCGCCAAATTCATCGtcatcatca                                                                                       >  1:120062/1‑150 (MQ=255)
                                                                        cccGTCTAAATTATCGTATTTGAGTTAATCTTCGCCCAGCGCCTGTTTGGCTTTTATTGACGTTAACGCCCGCCAGGAATAATCGCGACTCCACTCCCATTTTTTTTGTGTGTCATCGCCGCCAAATTCATCGTCATCATCATTACTgcg                                                                               <  1:3709209/149‑1 (MQ=255)
                                                                          cGTCGAAAATATCGGAATTGAGGTAATCTTCGCCCAGCGCCAGTTTGGCTTTTAATGACGGTAACGCCCGCCAGGCATAATAGCGACTCCACTCCCATTTTTTTTGTGTGTCATCGCCGCCAAATTCATCGTCATCATCATTACTGcgcg                                                                             <  2:8139648/150‑1 (MQ=255)
                                                                            tCGAAAATATCGGAATTGAGGTAATCTTCGCCCAGCGCCAGTTTGGCTTTTAATGACGGTAACGCCCGCCAGGCATAATAGCGACTCCACTCCCATTTTTTTTGTGTGTCATCGCCGCCAAATTCATCGTCATCATCATTACTGCGCGTa                                                                           >  1:7889530/1‑150 (MQ=255)
                                                                             cGAAAATATCGGAATGGTGGTAATCTGCGCCCTGCGCCAGTTGGGCTTTTACTGACGGTAACGCCCGCCTGGCATAATAGCGACTCCACTCCCATTTTTTTTGTGTGTCATCGCCGCCAAATTCATCGTCATCATCATTACTGCGCGTAt                                                                          <  1:8114766/150‑1 (MQ=255)
                                                                              gAAAATATCGGAATTGAGGTAATCTTCGCCCAGCGCCAGTTTGGCTTTTAATGACGGTAACGCCCGCCAGGCATAATAGCGACTCCACTCCCATTTTTTTTGTGTGTCATCGCCGCCAAATTCATCGTCATCATCATTACTGCGCGTATg                                                                         >  1:9927814/1‑150 (MQ=255)
                                                                                 actatCGGAATTGTGGTAATCTTCGCCCAGCGCCAGTTTGGCTTTTAATGACGGTAACGCCCGCCAGGCATAATAGCGACTCCACTCCCATTTTTTTTGTGTGTCATCGCCGCCAAATTCATCGTCATCATCATTACTGCGCGTATGTTg                                                                      <  2:10155363/148‑1 (MQ=255)
                                                                                   tatCGGAATTGAGGTAATCTTCGCCCAGCGCCAGTTGGGCTTTTAATGACGGTAACGCCCGCCAGGCATAATAGCGACTCCACTCCCATTTTTTTTGTGTGTCATCGCCGCCAAATTCATCGTCATCATCATTACTGCGCGTATGTTGAt                                                                    <  1:9595462/150‑1 (MQ=255)
                                                                                    atCGGAATTGAGGTAATCTTCGCCCAGCGCCAGTTTGGCTTTTAATGACGGTAACGCCCGCCAGGCATAATAGCGACTCCACTCCCATTTTTTTTGTGTGTCATCGCCGCCAAATTCATCGTCATCATCATTACTGCGCGTATGTTGATa                                                                   >  2:7279751/1‑150 (MQ=255)
                                                                                    atCGGAATGGAGGTAATCTTCGCCCAGCGCCAGTTTGGCTTTTAATGACGGTAACGCCCGCCAGGCATAATAGCGACTCCACTCCCATTTTTTTTGTGTGTCATCGCCGCCAAATTCATCGTCATCATCATTACTGCGCGTATGTTGATa                                                                   <  2:8721084/150‑1 (MQ=255)
                                                                                     tCGGAATTGAGGTAATCTTCGCCCAGCGCCAGTTTGGCTTTTAATGACGGTAACGCCCGCCAGGCATAATAGCGACTCCACTCCCATTTTTTTTGTGTGTCATCGCCGCCAAATTCATCGTCATCATCATTACTGCGCGTATGTTGATAg                                                                  >  2:12178782/1‑150 (MQ=255)
                                                                                       ggAATTGAGGTAATCTTCGCCCAGCGCCAGTTTGGCTTTTAATGACGGTAACGCCCGCCAGGCATAATAGCGACTCCACTCCCATTTTTTTTGTGTGTCATCGCCGCCAAATTCATCGTCATCATCATTACTGCGCGTATGTTGATAGtt                                                                >  1:10283531/1‑150 (MQ=255)
                                                                                         aaTTGAGGTAATCTTCGCCCAGCGCCAGTTTGGCTTTTAATGACGGTAACGCCCGCCAGGCATAATAGCGACTCCACTCCCATTTTTTTTGTGTGTCATCGCCGCCAAATTCATCGTCATCATCATTACTGCGCGTATGTTGATAGTTgg                                                              >  1:4144330/1‑150 (MQ=255)
                                                                                            tGAGGTAATCTTCGCCCAGCGCCAGTTTGGCTTTTAATGACGGTAACGCCCGCCAGGCATAATAGCGACTCCACTCCCATTTTTTTTGTGTGTCATCGCCGCCAAATTCATCGTCATCATCATTACTGCGCGTATGTTGATAGTTGGTCt                                                           <  2:6396048/150‑1 (MQ=255)
                                                                                             gAGGTAATCTTCGCCCAGCGCCAGTTTGGCTTTTAATGACGGTAACGCCCGCCAGGCATAATAGCGACTCCACTCCCATTTTTTTTGTGTGTCATCGCCGCCAAATTCATCGTCATCATCATTACTGCGCGTATGTTGATAGTTGGTCTg                                                          <  2:3903175/150‑1 (MQ=255)
                                                                                             gAGGTAATCTCCGCCCAGCGCCAGTTTGGCTTTTAATGACGGTAACGCCCGCCAGGAATAATCGCGACTCCACTCCCATTTTTTTTGTGTGTCATCGCCGCCAAATTCATCGTCATCATCATTACTGCGCGTATGTTGATAGTTGGTCTg                                                          <  1:8293363/150‑1 (MQ=255)
                                                                                              aGGTAATCTTCGCCCAGCGCCAGTTTGGCTTTTAATGACGGTAACGCCCGCCAGGCATAATAGCGACTCCACTCCCATTTTTTTTGTGTGTCATCGCCGCCAAATTCATCGTCATCATCATTACTGCGCGTATGTTGATAGTTGGTCTGc                                                         >  1:7367068/1‑150 (MQ=255)
                                                                                                gTAATCTTCGCCCAGCGCCAGTTTGGCTTTTAATGAGGGTACCGCCCGCCAGGAATAATAGCGACTCCACTCCCATTTTTTTTGTGTGTCATCGCCGCCAAATTCATCGTCATCATCATTACTGCGCGTATGTTGATAGTTGGTCTGCCa                                                       <  1:10444103/150‑1 (MQ=255)
                                                                                                gTAATCTTCGCCCAGCGCCAGTTTGGCTTTTAATGACGGTAACGCCCGCCAGGCATAATAGCGACTCCACTCCCATTTTTTTTGTGTGTCATCGCCGCCAAATTCATCGTCATCATCATTACTGCGCGTATGTTGATAGTTGGTCTGCCa                                                       >  1:853422/1‑150 (MQ=255)
                                                                                                gTAATCTTCGCCCAGCGCCAGTTTGGCTTTTAATGACGGTAACGCCCGCCAGGCATAATAGCGACTCCACTCCCATTTTTTTTGTGTGTCATCGCCGCCAAATTCATCGTCATCATCATTACTGCGCGTATGTTGATAGTTGGTCTGCCa                                                       >  1:4625929/1‑150 (MQ=255)
                                                                                                    tCTTCGCCCAGCGCCAGTTTGGCTTTTAATGACGGTAACGCCCGCCAGGCATAATAGCGACTCCACTCCCATTTTTTTTGTGTGTCATCGCCGCCAAATTCATAGTCATCATCATTACTGCGCGTATGTTGATAGTTGGTCTGCCAGTCa                                                   >  1:484371/1‑150 (MQ=255)
                                                                                                     cTTCGCCCAGCGCCAGTTTGGCTTTTAATGACGGTAACGCCCGCCAGGCATAATAGCGACTCCACTCCCATTTTTTTTGTGTGTCATCGCCGCCAAATTCATCGTCATCATCATTACTGCGCGTATGTTGATAGTTGGTCTGCCAGTCAg                                                  >  1:10669294/1‑150 (MQ=255)
                                                                                                      ttCGCCCAGCGCCAGTTTGGCTTTTAATGACGGTAACGCCCGCCAGGCATAATAGCGACTCCACTCCCATTTTTTTTGTGTGTCATCGCCGCCAAATTCATCGTCATCATCATTACTGCGCGTATGTTGATAGTTGGTCTGCCAGTCAgc                                                 >  1:4409315/1‑150 (MQ=255)
                                                                                                      ttCGCCCAGCGCCAGTTTGGCTTTTAATGACGGTAACGCCCGCCAGGCATAATAGCGACTCCACTCCCATTTTTTTTGTGTGTCATCGCCGCCAAATTCATCGTCATCATCATTACTGCGCGTATGTTGATAGTTGGTCTGCCAGTCAgc                                                 >  2:6299491/1‑150 (MQ=255)
                                                                                                       tCGCCCAGCGCCAGTTTGGCTTTTAATGACGGTAACGCCCGCCAGGCATAATAGCGACTCCACTCCCATTTTTTTTGTGTGTCATCGCCGCCAAATTCATCGTCATCATCATTACTGCGCGTATGTCGATAGTTGGTCTGCCAGTCAgcg                                                >  1:6026974/1‑150 (MQ=255)
                                                                                                          cccAGCGCCAGTTTGGCTTTTAATGACGGTAACGCCCGCCAGGCATAATAGCGACTCCACTCCCATTTTTTTTGTGTGTCATCGCCGCCAAATTCATCGTCATCATCATTACTGCGCGTATGTTGATAGTTGGTCTGCCAGTCAgcgcgc                                             <  2:826334/150‑1 (MQ=255)
                                                                                                           ccAGCGCCAGTTTGGCTTTTAATGACGGTAACGCCCGCCAGGCATAATAGCGACTCCACTCCCATTTTTTTTGTGTGTCATCGCCGCCAAATTCATCGTCATCATCATTACTGCGCGTATGTTGGTAGTTGGTCTGCCAGTCAGCGCGCa                                            >  1:6404302/1‑150 (MQ=255)
                                                                                                           ccAGCGCCAGTTTGGCTTTTAATGACGGTAACGCCCGCCAGGCATAATAGCGACACCACTCCCATTTTTTTTGTGTGTCATCGCCGCCCAATTCATCGTCATCATCATTACTGCGCGTCTGTTGATAGTTGGTCTGCCAGTCAgcgcgcc                                            >  1:5227433/1‑149 (MQ=255)
                                                                                                                 cctttttGGATTTTAATGACGTTAACGCCCGCCAGGAATAATAGCGACTCCACTCCCATTTTTTTTGTGTGTCATCGCCGCCAAATTCATCGTCATCATCATTACTGCGCGTATGTTGATAGTTGGTCTGCCAGTCAGCGCGCATACGcc                                      <  2:1071845/146‑1 (MQ=255)
                                                                                                                  ctgTTTGGATTTTAATGACGGTAACGCCCGCCAGGAATAATAGCGACTCCACTCCCATTTTTTTTGTGTGTCATCGCCGCCAAATTCATCGTCATCATCATTACTGCGCGTATGTTGATAGTTGGTCTGCCAGTCAGCGCGCATACGCCa                                     <  1:11827042/148‑1 (MQ=255)
                                                                                                                     tttGGCTTTTAATGACGGTAACGCCCGCCAGGCATAATAGCGACTCCACTCCCATTTTTTTTGTGTGTCATCGCCGCCAAATTCATCGTCATCATCATTACTGCGCGTATGTTGATAGTTGGTCTGCCAGTCAGCGCGCATACGCCATgg                                  >  1:11643470/1‑150 (MQ=255)
                                                                                                                     tttGGCTTTTAATGACGGTAACGCCCGCCAGGCATAATAGCGACTCCACTCCCATTTTTTTTGTGTGTCATCGCCGCCAAATTCATCGTCATCATCATTACTGCGCGTATGTTGATAGTTGGTCTGACAGTCAGCGCGCATACGCCATgg                                  <  2:10988747/150‑1 (MQ=255)
                                                                                                                     tttGGCTTTTAATGACGGTAACGCCCGCCAGGCATAATAGCGACTCCACTCCCATCTTTTTTGTGTGTCATCGCCGCCAAATTCATCGTCATCATCATTACTGCGCGTATGTTGATAGTTGGTCTGCCAGTCAGCGCGCATACGCCATgg                                  >  2:8480789/1‑150 (MQ=255)
                                                                                                                           ttttAATGACGGTAACGCCCGCCAGGCATAATAGCGACTCCACTCCCATTTTTTTTGTGTGTCATCGCCGCCAAATTCATCGTCATCATCATTACTGCGCGTATGTTGATAGTTGGTCTGCCAGTCAGCGCGCATACGCCATGGCCCCAg                            <  1:9177220/150‑1 (MQ=255)
                                                                                                                           ttttAATGACGGTAACGCCCGCCAGGCATAATAGCGACTCCACTCCCATTTTTTTTGTGTGTCATCGCCGCCAAATTCATCGTCATCATCATTACTGCGCGTATGTTGATAGTTGGTCTGCCAGTCAGCGCGCATACGCCATGGCCCCAg                            >  1:11010428/1‑150 (MQ=255)
                                                                                                                                aTGACGGTAACGCCCGCCAGGCATAATAGCGACTCCACTCCCATTTTTTTTGTGTGTCATCGCCGCCAAATTCATCGTCATCATCATTACTGCGCGTATGTTGGTAGTTGGTCTg                                                          >  1:10210146/1‑115 (MQ=255)
                                                                                                                                aTGACGGTAACGCCCGCCAGGCATAATAGCGACTCCACTCCCATTTTTTTTGTGTGTCATCGCCGCCAAATTCATCGTCATCATCATTACTGCGCGTATGTTGATAGTTGGTCTGCCAGTCAGCGCGCATACGCCATGGCCCCAGGTTaa                       <  1:5427794/150‑1 (MQ=255)
                                                                                                                                aTGACGGTAACGCCCGCCAGGCATAATAGCGACTCCACTCCCATTTTTTTTGTGTGTCATCGCCGCCAAATTCATCGTCATCATCATTACTGCGCGTATGTTGATAGTTGGTCTGCCAGTCAGCGCGCATACGCCATGGCCCCAGGTTaa                       <  1:2284650/150‑1 (MQ=255)
                                                                                                                                 tGACGGTAACGCCCGCCAGGCATAATAGCGACTCCACTCCCATTTTTTTTGTGTGTCATCGCCGCCAAATTCATCGTCATCATCATTACTGCGCGTATGTTGATAGTTGGTCTGCCAGTCAGCGCGCATACGCCATGGCCCCAGGTTAAc                      >  2:4997856/1‑150 (MQ=255)
                                                                                                                                 tGACGGTAACGCCCGCCAGGCATAATAGCGACTCCACTCCCATTTTTTTTGTGTGTCATCGCCGCCAAATTCATCGTCATCATCATTACTGCGCGTATGTTGATAGTTGGTCTGCCAGTCAGCGCGCATACGCCATGGCCCCAGGTTAAc                      >  1:87584/1‑150 (MQ=255)
                                                                                                                                    cGGTAACGCCCGCCAGGCATAATAGCGACTCCACTCCCATTTTTTTTGTGTGTCATCGCCGCCAAATTCATCGTCATCATCATTACTGCGCGTATGTTGATAGTTGGTCTGCCAGTCAGCGCGCATACGCCATGGCCCCAGGTTAAcccc                   >  2:10277313/1‑150 (MQ=255)
                                                                                                                                     ggTAACGCCCGCCAGGCATAATAGCGACTCCACTCCCATTTTTTTTGTGTGTCATCGCCGCCAAATTCATCGTCATCATCATTACTGCGCGTATGTTGATAGTTGGTCTGCCAGTCAGCGCGCATACGCCATGGCCCCAGGTTAACCCCg                  >  2:8997609/1‑150 (MQ=255)
                                                                                                                                      gTAACGCCCGCCAGGCATAATAGCGACTCCACTCCCATTTTTTTTGTGTGTCATCGCCGCCAAATTCATCGTCATCATCATTACTGCGCGTATGTTGATAGTTGGTCTGCCAGTCAGCGCGCATACGCCATGGCCCCAGGTTAACCCCGa                 <  1:6575185/150‑1 (MQ=255)
                                                                                                                                      gTAACGCCCGCCAGGCATAATAGCGACTCCACTCCCATTTTTTTTGTGTGTCATCGCCGCCAAATTCATCGTCATCATCATTACTGCGCGTATGTTGATAGTTGGTCTGCCAGTCAGCGCGCATACGCCATGGCCCCAGGTTAACCCCGa                 <  2:7913632/150‑1 (MQ=255)
                                                                                                                                          cgcccgccAGGCATAATAGCGACTCCACTCCCATTTTTTTTGTGTGTCATCGCCGCCAAATTCATCGTCATCATCATTACTGCGCGTATGTTGATAGTTGGTCTGCCAGTCAGCGCGCATACGCCATGGCCCCAGGTTAACCCCGACCGt             >  2:11555258/1‑150 (MQ=255)
                                                                                                                                            cccgccAGGCATAATATCGACTCCACTCCCATTTTTTTTTGTGTGTCATCGCCGCCAAATTCATCGTCATCATCATTACTGCGCGTATGTTGATAGTTGGTCTGCCAGTCAGCGCGCATACGCCATGGCCCCAGGTTAACCCCGACCGTc            <  2:7077239/150‑1 (MQ=255)
                                                                                                                                             ccgccAGGCATAATCGCGACTCCACTCCCATTTTTTTTGTGTGTCATCGCCGCCAAATTCATCGTCATCATCATTACTGCGCGTATGTTGATAGTTGGTCTGCCAGTCAGCGCGCATACGCCATGGCCCCAGGTTAACCCCGACCGTccc          <  2:5434552/150‑1 (MQ=255)
                                                                                                                                             ccgccAGGCATAATAGCGACTCCACTCCCATTTTTTTTGTGTGTCATCGCCGCCAAATTCATCGTCATCATCATTACTGCGCGTATGTTGATAGTTGGTCTGCCAGTCAGCGCGCATACGCCATGGCCCCAGGTTAACCCCGACCGTccc          <  1:12236013/150‑1 (MQ=255)
                                                                                                                                              cgccAGGCATAATAGCGACTCCACTCCCATTTTTTTTGTGTGTCATCGCCGCCAAATTCATCGTCATCATCATTACTGCGCGTATGTTGATAGTTGGTCTGCCAGTCAGCGCGCATACGCCATGGCCCCAGGTTAACCCCGACCGTCCCg         >  2:3020801/1‑150 (MQ=255)
                                                                                                                                              cgccAGGCATAATAGCGACTCCACTCCCATTTGTTTTGGGTGTCATCGCCGCCAAATTCATCGTCATCATCATTACTGCGCGTATGTTGATAGTTGGTCTGCCAGTCAGCGCGCATACGCCATGGCCCCAGGTTAACCCCGACCGTCCCg         >  2:11336786/1‑150 (MQ=255)
                                                                                                                                               gccCGGCTTAATCGCGACTCCACTCACATTTTTTTTGTGTGTCATCGCCGCCAAATTCATCGTCATCATCATTACCGCGCGTATGTTGATAGTTGGTCTGCCAGTCAGCGCGCATACGCCATGGCCCCAGGTTAACCCCGACCGTCCCGt        <  2:9290814/150‑1 (MQ=255)
                                                                                                                                               gccAGGCATAATAGCGACTCCACTCCCATTTTTTTTGTGTGTCATCGCCGCCAAATTCATCGTCATCATCATTACTGCGCGTATGTTGATAGTTGGTCTGCCAGTCAGCGCGCATACGCCATGGCCCCAGGTTAACCCCGACCGTCCCGt        <  1:3486638/150‑1 (MQ=255)
                                                                                                                                                ccAGGCATAATAGCGACTCCACTCCCATTTTTTTTGTGTGTCATCGCCGCCAAATTCATCGTCATCATCATTACTGCGCGTATGTTGATAGTTGGTCTGCCAGTCAGCGCGCATACGCCATGGCCCCAGGTTAACCCCGACCGTCCCGtt       <  2:11967437/150‑1 (MQ=255)
                                                                                                                                                ccAGGCAAAATAGCGACTCCACTCCCATTTTTTTTGTGTGTCATCGCCGCCAAATTCATCGTCATCATCATTACTGCGCGTATGTTGATAGTTGGTCTGCCAGTCAGCGCGCATACGCCATGGCCCCAGGTTAACCCCGACCGTCCCGtt       <  2:7439841/150‑1 (MQ=255)
                                                                                                                                                 cAGGCATAATAGCGACTCCACTCCCATTTTTTTTGTGTGTCATCGCCGCCAAATTCATCGTCATCATCATTACTGCGCGTATGTTGATAGTTGGTCTGCCAGTCAGCGCGCATACGCCATGGCCCCAGGTTAACCCCGACCGTCCCGTTg      <  1:1561257/150‑1 (MQ=255)
                                                                                                                                                  aGGCATAATAGCGACTCCACTCCCATTTTTTTTGTGTGTCATCGCCGCCAAATTCATCGTCATCATCATTACTGCGCGTATGTTGATAGTTGGTCTGCCAGTCAGCGCGCATACGCCATGGCCCCAGGTTAACCCCGACCGTCCCGTTGc     >  2:1164951/1‑150 (MQ=255)
                                                                                                                                                   ggCATAATAGCGACTCCACTCCCATTTTTTTTGTGTGTCATCGCCGCCAAATTCATCGTCATCATCATTACTGCGCGTATGTTGATAGTTGGTCTGCCAGTCAGCGCGCATACGCCATGGCCCCAGGTTAACCCCGACCGTCCCGTTGcc    <  1:953242/150‑1 (MQ=255)
                                                                                                                                                    gCATAATAGCGACTCCACTCCCATTTTTTTTGTGTGTCATCGCCGCCAAATTCATCGTCATCATCATTACTGCGCGTATGTTGATAGTTGGTCTGCCAGTCAGCGCGCATACGCCATGGCCCCAGGTTAACCCCGACCGTCCCGTTGCCg   >  2:4165194/1‑150 (MQ=255)
                                                                                                                                                     cATAATAGCGACTCCACTCCCATTTTTTTTGTGTGTCATCGCCGCCAAATTCATCGTCATCATCATTAGTGCGCGTATGTTGATAGTTGGTGTGCCAGTCAGCGCGCATACGCCATGGCCCCAGGTTAACCCCGACCGTCGCGTTGCCGc  >  2:8598587/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                      
TGGCGCGTAGCCGCGCAGATTGGGAGGCAACATTTGATCGTCAGTGCTGACACTGCCACCAACATAATTAAAACCGTCGAAAATATCGGAATTGAGGTAATCTTCGCCCAGCGCCAGTTTGGCTTTTAATGACGGTAACGCCCGCCAGGCATAATAGCGACTCCACTCCCATTTTTTTTGTGTGTCATCGCCGCCAAATTCATCGTCATCATCATTACTGCGCGTATGTTGATAGTTGGTCTGCCAGTCAGCGCGCATACGCCATGGCCCCAGGTTAACCCCGACCGTCCCGTTGCCGC  >  NC_000913/2454117‑2454415

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: